pycom14g20290

Protein SHOOT GRAVITROPISM 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0006002
Physical Location & Seq
Reverse (-)
21354767 .. 21356824
2058 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g20290.1

Sequence Viewer

Length: 783 bp
ATGTTACCCAATAACTCTTCTTCCTTGGTCCCTTCTTCTGATCCTTTTTCTTGCTTAGAAAATGGCAACACCAATAAAAGAAAGAGAAGACCTGCAGGCACTCCAGATCCAGATGCGGAGGTGGTGTCACTTTCACCGAAAACCTTATTGGAATCGGATCGTTACATTTGCGAGATCTGCAACCAAGGGTTTCAGAGAGACCAGAACCTACAGATGCACCGGCGGCGGCACAAGGTGCCATGGAAGCTGCTGAAGCGAGAGACTCCGGTCGTGAAGAAGCGGGTGTTCGTGTGCCCAGAACCAAGCTGCCTGCACCATGATCCCTGCCACGCCCTCGGCGATCTCGTCGGTATCAAAAAGCATTTCAGGAGAAAGCACAGCAACCACAAACAGTGGGTTTGTGATAAGTGTTCAAAAGGGTATGCAGTTCAATCAGATTACAAGGCACATCTCAAAACCTGCGGCACCAGAGGACATTCTTGCGACTGCGGCCGTGTTTTCTCTAGGGTTGAGAGTTTCATAGAGCACCAAGACGCTTGCAACATGGATCGTGTGCGGCCGGAATCACAAACGCTGCAACCGGCAGCATGCCTGTCACGAACGGCTTCAAGTCCTAGTCCATCCTGCAGTGACAACAATTTCAGCAGGGCTCCATCCACTTGGCCTCCATCTAAGAATTTGGCACTACCACATCCAAGTCCAACTGAGTACACCAAATTAATCTTAAGAAACCCTCCTGACCACGCTGATCGATCATCAAGCTACACTGTCCAAGAAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C2H2

Protein Analysis

261

Amino Acids

29.4

Weight (kDa)

9.15

Isoelectric Point (pI)

80.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2H2-2nd_BIRD-IDD PF22996 92 - 126 2e-18 BIRD-IDD transcription factor, second C2H2 zinc finger
C2CH-3rd_BIRD-IDD PF22995 131 - 155 1.9e-14 BIRD-IDD transcription factor, third C2HC zinc finger
C2CH-4th_BIRD-IDD PF22992 159 - 181 1e-06 BIRD-IDD transcription factor, fourth C2HC zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 100, 467
AccB1I GGYRCC 2 cut(s) 235, 464
AciI CCGC 7 cut(s) 116, 223, 226, 280, 462, 489, 556
AclWI GGATC 5 cut(s) 35, 101, 165, 314, 555
AcoI YGGCCR 2 cut(s) 490, 557
AcsI RAATTY 1 cut(s) 676
AcuI CTGAAG 1 cut(s) 272
AdeI CACNNNGTG 1 cut(s) 235
AfaI GTAC 1 cut(s) 710
AflII CTTAAG 1 cut(s) 724
AgsI TTSAA 3 cut(s) 414, 431, 609
AjuI GAANNNNNNNTTGG 2 cut(s) 131, 163
AluBI AGCT 3 cut(s) 247, 306, 762
AluI AGCT 3 cut(s) 247, 306, 762
Alw21I GWGCWC 1 cut(s) 528
Alw26I GTCTC 2 cut(s) 192, 254
AlwI GGATC 5 cut(s) 35, 101, 165, 314, 555
AoxI GGCC 3 cut(s) 490, 557, 662
ApeKI GCWGC 4 cut(s) 247, 306, 574, 584
ApoI RAATTY 1 cut(s) 676
AseI ATTAAT 1 cut(s) 719
Asp700I GAANNNNTTC 1 cut(s) 604
AspS9I GGNCC 1 cut(s) 28
AsuHPI GGTGA 1 cut(s) 126
AvaII GGWCC 1 cut(s) 28
BaeGI GKGCMC 1 cut(s) 296
BanI GGYRCC 2 cut(s) 235, 464
BanII GRGCYC 1 cut(s) 652
BbsI GAAGAC 1 cut(s) 94
Bbv12I GWGCWC 1 cut(s) 528
BbvI GCAGC 4 cut(s) 234, 293, 561, 596
BccI CCATC 3 cut(s) 628, 661, 676
BceAI ACGGC 2 cut(s) 477, 618
BcoDI GTCTC 2 cut(s) 192, 254
BfaI CTAG 2 cut(s) 504, 615
BfmI CTRYAG 3 cut(s) 93, 209, 625
BfrI CTTAAG 1 cut(s) 724
BfuAI ACCTGC 2 cut(s) 100, 467
BglII AGATCT 1 cut(s) 174
BisI GCNGC 9 cut(s) 224, 227, 248, 307, 463, 490, 557, 575, 585
BlsI GCNGC 9 cut(s) 225, 228, 249, 308, 464, 491, 558, 576, 586
Bme18I GGWCC 1 cut(s) 28
BmgT120I GGNCC 1 cut(s) 28
BmiI GGNNCC 4 cut(s) 30, 237, 466, 651
BmsI GCATC 2 cut(s) 103, 204
BoxI GACNNNNGTC 1 cut(s) 266
BpiI GAAGAC 1 cut(s) 94
BpmI CTGGAG 1 cut(s) 87
Bsa29I ATCGAT 1 cut(s) 751
BsaI GGTCTC 1 cut(s) 192
BsaJI CCNNGG 4 cut(s) 24, 184, 239, 334
BsaWI WCCGGW 1 cut(s) 265
BsaXI ACNNNNNCTCC 4 cut(s) 110, 140, 649, 679
Bse118I RCCGGY 2 cut(s) 219, 580
BseCI ATCGAT 1 cut(s) 751
BseDI CCNNGG 4 cut(s) 24, 184, 239, 334
BseGI GGATG 3 cut(s) 620, 653, 691
BseMII CTCAG 1 cut(s) 696
BseSI GKGCMC 1 cut(s) 296
BseX3I CGGCCG 2 cut(s) 490, 557
BseXI GCAGC 4 cut(s) 234, 293, 561, 596
BsgI GTGCAG 1 cut(s) 296
Bsh1285I CGRYCG 3 cut(s) 270, 493, 560
BshFI GGCC 3 cut(s) 492, 559, 664
BshNI GGYRCC 2 cut(s) 235, 464
BshVI ATCGAT 1 cut(s) 751
BsiEI CGRYCG 3 cut(s) 270, 493, 560
BsiHKAI GWGCWC 1 cut(s) 528
BsiSI CCGG 4 cut(s) 220, 266, 560, 581
BslFI GGGAC 1 cut(s) 14
BsmAI GTCTC 2 cut(s) 192, 254
BsmFI GGGAC 1 cut(s) 14
BsnI GGCC 3 cut(s) 492, 559, 664
Bso31I GGTCTC 1 cut(s) 192
Bsp1286I GDGCHC 3 cut(s) 296, 528, 652
Bsp143I GATC 9 cut(s) 40, 106, 157, 174, 319, 340, 547, 748, 752
Bsp19I CCATGG 1 cut(s) 239
BspACI CCGC 7 cut(s) 116, 223, 226, 280, 462, 489, 556
BspANI GGCC 3 cut(s) 492, 559, 664
BspCNI CTCAG 1 cut(s) 697
BspDI ATCGAT 1 cut(s) 751
BspLI GGNNCC 4 cut(s) 30, 237, 466, 651
BspMAI CTGCAG 2 cut(s) 97, 629
BspMI ACCTGC 2 cut(s) 100, 467
BspPI GGATC 5 cut(s) 35, 101, 165, 314, 555
BspT107I GGYRCC 2 cut(s) 235, 464
BspTI CTTAAG 1 cut(s) 724
BspTNI GGTCTC 1 cut(s) 192
BsrFI RCCGGY 2 cut(s) 219, 580
BssAI RCCGGY 2 cut(s) 219, 580
BssECI CCNNGG 4 cut(s) 24, 184, 239, 334
BssMI GATC 9 cut(s) 40, 106, 157, 174, 319, 340, 547, 748, 752
BssT1I CCWWGG 3 cut(s) 24, 184, 239
Bst4CI ACNGT 2 cut(s) 393, 769
Bst6I CTCTTC 1 cut(s) 22
BstAFI CTTAAG 1 cut(s) 724
BstAPI GCANNNNNTGC 1 cut(s) 235
BstC8I GCNNGC 4 cut(s) 97, 311, 538, 589
BstDEI CTNAG 3 cut(s) 55, 672, 705
BstDSI CCRYGG 1 cut(s) 239
BstF5I GGATG 3 cut(s) 620, 653, 691
BstKTI GATC 9 cut(s) 43, 109, 160, 177, 322, 343, 550, 751, 755
BstMAI GTCTC 2 cut(s) 192, 254
BstMBI GATC 9 cut(s) 40, 106, 157, 174, 319, 340, 547, 748, 752
BstMCI CGRYCG 3 cut(s) 270, 493, 560
BstMWI GCNNNNNNNGC 6 cut(s) 177, 223, 235, 244, 253, 489
BstNSI RCATGY 1 cut(s) 591
BstPAI GACNNNNGTC 1 cut(s) 266
BstSFI CTRYAG 3 cut(s) 93, 209, 625
BstSLI GKGCMC 1 cut(s) 296
BstV1I GCAGC 4 cut(s) 234, 293, 561, 596
BstV2I GAAGAC 1 cut(s) 94
BstX2I RGATCY 2 cut(s) 106, 174
BstXI CCANNNNNNTGG 1 cut(s) 660
BstYI RGATCY 2 cut(s) 106, 174
BstZI CGGCCG 2 cut(s) 490, 557
Bsu15I ATCGAT 1 cut(s) 751
BsuRI GGCC 3 cut(s) 492, 559, 664
BsuTUI ATCGAT 1 cut(s) 751
BtgI CCRYGG 1 cut(s) 239
BtsCI GGATG 3 cut(s) 620, 653, 691
BtsI GCAGTG 1 cut(s) 634
BtsIMutI CAGTG 3 cut(s) 398, 634, 765
BveI ACCTGC 2 cut(s) 100, 467
Cac8I GCNNGC 4 cut(s) 97, 311, 538, 589
Cfr10I RCCGGY 2 cut(s) 219, 580
Cfr13I GGNCC 1 cut(s) 28
ClaI ATCGAT 1 cut(s) 751
CseI GACGC 1 cut(s) 542
Csp6I GTAC 1 cut(s) 709
CviAII CATG 4 cut(s) 240, 317, 544, 588
CviJI RGCY 8 cut(s) 247, 306, 492, 559, 605, 650, 664, 762
CviKI_1 RGCY 8 cut(s) 247, 306, 492, 559, 605, 650, 664, 762
CviQI GTAC 1 cut(s) 709
DdeI CTNAG 3 cut(s) 55, 672, 705
DpnI GATC 9 cut(s) 42, 108, 159, 176, 321, 342, 549, 750, 754
DpnII GATC 9 cut(s) 40, 106, 157, 174, 319, 340, 547, 748, 752
DraIII CACNNNGTG 1 cut(s) 235
EaeI YGGCCR 2 cut(s) 490, 557
EagI CGGCCG 2 cut(s) 490, 557
Eam1104I CTCTTC 1 cut(s) 22
EarI CTCTTC 1 cut(s) 22
EclXI CGGCCG 2 cut(s) 490, 557
Eco130I CCWWGG 3 cut(s) 24, 184, 239
Eco24I GRGCYC 1 cut(s) 652
Eco31I GGTCTC 1 cut(s) 192
Eco47I GGWCC 1 cut(s) 28
Eco52I CGGCCG 2 cut(s) 490, 557
Eco57I CTGAAG 1 cut(s) 272
EcoT14I CCWWGG 3 cut(s) 24, 184, 239
EcoT38I GRGCYC 1 cut(s) 652
ErhI CCWWGG 3 cut(s) 24, 184, 239
FaeI CATG 4 cut(s) 243, 320, 547, 591
FaiI YATR 6 cut(s) 241, 318, 423, 521, 545, 589
FaqI GGGAC 1 cut(s) 14
FatI CATG 4 cut(s) 239, 316, 543, 587
FauI CCCGC 1 cut(s) 273
Fnu4HI GCNGC 9 cut(s) 224, 227, 248, 307, 463, 490, 557, 575, 585
FokI GGATG 3 cut(s) 607, 640, 678
FriOI GRGCYC 1 cut(s) 652
Fsp4HI GCNGC 9 cut(s) 224, 227, 248, 307, 463, 490, 557, 575, 585
FspBI CTAG 2 cut(s) 504, 615
GluI GCNGC 9 cut(s) 224, 227, 248, 307, 463, 490, 557, 575, 585
GsuI CTGGAG 1 cut(s) 87
HaeIII GGCC 3 cut(s) 492, 559, 664
HapII CCGG 4 cut(s) 220, 266, 560, 581
HgaI GACGC 1 cut(s) 542
Hin1II CATG 4 cut(s) 243, 320, 547, 591
HinfI GANTC 3 cut(s) 152, 262, 563
HpaII CCGG 4 cut(s) 220, 266, 560, 581
HphI GGTGA 1 cut(s) 126
Hpy166II GTNNAC 1 cut(s) 711
Hpy188I TCNGA 4 cut(s) 40, 157, 195, 436
Hpy188III TCNNGA 6 cut(s) 104, 110, 271, 367, 597, 737
Hpy8I GTNNAC 1 cut(s) 711
Hpy99I CGWCG 1 cut(s) 350
HpyAV CCTTC 1 cut(s) 42
HpyCH4III ACNGT 2 cut(s) 393, 769
HpyCH4V TGCA 8 cut(s) 95, 180, 217, 313, 425, 540, 577, 627
HpyF10VI GCNNNNNNNGC 6 cut(s) 177, 223, 235, 244, 253, 489
HpyF3I CTNAG 3 cut(s) 55, 672, 705
Hsp92II CATG 4 cut(s) 243, 320, 547, 591
Kzo9I GATC 9 cut(s) 40, 106, 157, 174, 319, 340, 547, 748, 752
LmnI GCTCC 1 cut(s) 655
Lsp1109I GCAGC 4 cut(s) 234, 293, 561, 596
LweI GCATC 2 cut(s) 103, 204
MaeI CTAG 2 cut(s) 504, 615
MaeIII GTNAC 5 cut(s) 3, 126, 161, 594, 629
MalI GATC 9 cut(s) 42, 108, 159, 176, 321, 342, 549, 750, 754
MboI GATC 9 cut(s) 40, 106, 157, 174, 319, 340, 547, 748, 752
MboII GAAGA 5 cut(s) 9, 12, 27, 99, 286
MflI RGATCY 2 cut(s) 106, 174
MhlI GDGCHC 3 cut(s) 296, 528, 652
MluCI AATT 3 cut(s) 637, 676, 716
MlyI GAGTC 1 cut(s) 256
MmeI TCCRAC 1 cut(s) 725
MnlI CCTC 5 cut(s) 112, 344, 464, 675, 744
MroXI GAANNNNTTC 1 cut(s) 604
MseI TTAA 2 cut(s) 719, 725
MspCI CTTAAG 1 cut(s) 724
MspI CCGG 4 cut(s) 220, 266, 560, 581
MwoI GCNNNNNNNGC 6 cut(s) 177, 223, 235, 244, 253, 489
NcoI CCATGG 1 cut(s) 239
NdeII GATC 9 cut(s) 40, 106, 157, 174, 319, 340, 547, 748, 752
NlaIII CATG 4 cut(s) 243, 320, 547, 591
NlaIV GGNNCC 4 cut(s) 30, 237, 466, 651
NmeAIII GCCGAG 1 cut(s) 315
NmuCI GTSAC 3 cut(s) 126, 594, 629
NspI RCATGY 1 cut(s) 591
PaeI GCATGC 1 cut(s) 591
PcsI WCGNNNNNNNCGW 2 cut(s) 336, 342
PdmI GAANNNNTTC 1 cut(s) 604
PfeI GAWTC 2 cut(s) 152, 563
PkrI GCNGC 9 cut(s) 225, 228, 249, 308, 464, 491, 558, 576, 586
PleI GAGTC 1 cut(s) 256
PpsI GAGTC 1 cut(s) 256
PshAI GACNNNNGTC 1 cut(s) 266
PshBI ATTAAT 1 cut(s) 719
PspN4I GGNNCC 4 cut(s) 30, 237, 466, 651
PspPI GGNCC 1 cut(s) 28
PstI CTGCAG 2 cut(s) 97, 629
PsuI RGATCY 2 cut(s) 106, 174
RsaI GTAC 1 cut(s) 710
RsaNI GTAC 1 cut(s) 709
SaqAI TTAA 2 cut(s) 719, 725
SatI GCNGC 9 cut(s) 224, 227, 248, 307, 463, 490, 557, 575, 585
Sau3AI GATC 9 cut(s) 40, 106, 157, 174, 319, 340, 547, 748, 752
Sau96I GGNCC 1 cut(s) 28
SbfI CCTGCAGG 1 cut(s) 97
SchI GAGTC 1 cut(s) 256
SdaI CCTGCAGG 1 cut(s) 97
SduI GDGCHC 3 cut(s) 296, 528, 652
SetI ASST 9 cut(s) 94, 123, 146, 210, 237, 249, 308, 461, 764
SfaNI GCATC 2 cut(s) 103, 204
SfcI CTRYAG 3 cut(s) 93, 209, 625
SgrAI CRCCGGYG 1 cut(s) 219
SinI GGWCC 1 cut(s) 28
SmlI CTYRAG 1 cut(s) 724
SmoI CTYRAG 1 cut(s) 724
SphI GCATGC 1 cut(s) 591
Sse8387I CCTGCAGG 1 cut(s) 97
Sse9I AATT 3 cut(s) 637, 676, 716
SsiI CCGC 7 cut(s) 116, 223, 226, 280, 462, 489, 556
SspMI CTAG 2 cut(s) 504, 615
StyI CCWWGG 3 cut(s) 24, 184, 239
TaaI ACNGT 2 cut(s) 393, 769
TaqI TCGA 1 cut(s) 751
TasI AATT 3 cut(s) 637, 676, 716
TatI WGTACW 1 cut(s) 708
TauI GCSGC 5 cut(s) 226, 229, 465, 492, 559
TfiI GAWTC 2 cut(s) 152, 563
Tru1I TTAA 2 cut(s) 719, 725
Tru9I TTAA 2 cut(s) 719, 725
TscAI CASTG 3 cut(s) 398, 634, 772
TseFI GTSAC 3 cut(s) 126, 594, 629
TseI GCWGC 4 cut(s) 247, 306, 574, 584
Tsp45I GTSAC 3 cut(s) 126, 594, 629
TspDTI ATGAA 1 cut(s) 508
TspRI CASTG 3 cut(s) 398, 634, 772
Vha464I CTTAAG 1 cut(s) 724
VpaK11BI GGWCC 1 cut(s) 28
VspI ATTAAT 1 cut(s) 719
XapI RAATTY 1 cut(s) 676
XceI RCATGY 1 cut(s) 591
XmnI GAANNNNTTC 1 cut(s) 604
XspI CTAG 2 cut(s) 504, 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.