MD07G1011300.v1.1

Nuclear transcription factor Y subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007525
Physical Location & Seq
Forward (+)
1023634 .. 1029101
5468 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1011300.v1.1.491

Sequence Viewer

Length: 840 bp
ATGGTCATGAATGGAGCAATGCAGGCACAACCTAATGCTAGGCTGGACGGTGGTGTGGCCAACTTCCACAAAGAATTGCGGACTACAGTAGGATCACAGTCTGACGGGAATAATGGAAGTGAAAACGAACATATCAAAAGCGTTTCGTCGTCAGTGGTTCCTGCATTGGGTGAACACGTGGATCCAAATTCACAAATGGAACTTGTTGGCCACTCAATTGTTATGTCATCATATCCGTATTCAGATCCACAATATGGTGGGATGTTGACTCCTTATGGGCCACAAGCTATGTTGCCTCCCCACTTCTATGGTATGCATCCTGGTAGAATGCCTTTGCCCCTTGATATGGAAGAGGAGCCTGTGTATGTAAATGCGAAGCAGTACCATGGTATTTTGAGACGAAGACAGTCACGTGCTAAAGCTGAGCTTGAAAAGAAACTTATAAAAGCTCGAAAGCCTTATCTTCACGAGTCTCGACACCTACATGCATTAAGAAGGGCAAGGGGCTGTGGAGGCCGCTTCCTTAATACAAAGAAACAAGATGCCAATGACGAGAATTCCTCGTTAGAAAAAGGCATGAATTTGGATGGAAACCATTCAGCCCAATCCGCCAAATCTGGTTTTGAGTGCTGTCCAAATACCAGCAATGGGAATGTTGATTCCTCAAATGTCCAGCAAGACGGATCAGGTCCATGGTTCAGGACACACAACCACCACACACACTCTAATGGTAATGGCAACGATCATGGCCCATCATCAGCATACCCTTCCACGTTCAGTGACAGCAGGGAAAACATGCAGTTGAAAGGGTTGTCTCGTGGGGCCATTCCCAGTAAATGA

Protein Analysis

280

Amino Acids

30.58

Weight (kDa)

8.69

Isoelectric Point (pI)

50.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBFB_NFYA PF02045 119 - 174 5.3e-27 CCAAT-binding transcription factor (CBF-B/NF-YA) subunit B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 443
AccB7I CCANNNNNTGG 1 cut(s) 254
AciI CCGC 3 cut(s) 79, 517, 609
AclWI GGATC 5 cut(s) 100, 176, 189, 239, 691
AcoI YGGCCR 2 cut(s) 57, 208
AcsI RAATTY 3 cut(s) 187, 556, 580
AcvI CACGTG 2 cut(s) 178, 413
AfaI GTAC 1 cut(s) 383
AfiI CCNNNNNNNGG 4 cut(s) 167, 254, 346, 648
AflIII ACRYGT 1 cut(s) 175
AgsI TTSAA 2 cut(s) 431, 805
AjnI CCWGG 1 cut(s) 319
AluBI AGCT 4 cut(s) 287, 422, 427, 449
AluI AGCT 4 cut(s) 287, 422, 427, 449
Alw26I GTCTC 3 cut(s) 391, 477, 819
AlwI GGATC 5 cut(s) 100, 176, 189, 239, 691
AoxI GGCC 6 cut(s) 57, 208, 278, 514, 748, 822
ApoI RAATTY 3 cut(s) 187, 556, 580
Asp700I GAANNNNTTC 1 cut(s) 595
AspS9I GGNCC 4 cut(s) 278, 689, 749, 822
AsuHPI GGTGA 1 cut(s) 182
AvaII GGWCC 1 cut(s) 689
BalI TGGCCA 2 cut(s) 59, 210
BamHI GGATCC 1 cut(s) 181
BauI CACGAG 2 cut(s) 467, 816
BbrPI CACGTG 2 cut(s) 178, 413
BbsI GAAGAC 1 cut(s) 409
BccI CCATC 2 cut(s) 581, 760
BciT130I CCWGG 1 cut(s) 321
BcoDI GTCTC 3 cut(s) 391, 477, 819
BfaI CTAG 1 cut(s) 39
BfmI CTRYAG 1 cut(s) 84
BisI GCNGC 1 cut(s) 517
BlpI GCTNAGC 1 cut(s) 423
BlsI GCNGC 1 cut(s) 518
Bme1390I CCNGG 1 cut(s) 321
Bme18I GGWCC 1 cut(s) 689
BmgT120I GGNCC 4 cut(s) 278, 689, 749, 822
BmiI GGNNCC 4 cut(s) 159, 183, 357, 823
BmrFI CCNGG 1 cut(s) 321
BmrI ACTGGG 1 cut(s) 825
BmsI GCATC 2 cut(s) 325, 532
BmuI ACTGGG 1 cut(s) 825
BpiI GAAGAC 1 cut(s) 409
BplI GAGNNNNNCTC 2 cut(s) 545, 577
Bpu1102I GCTNAGC 1 cut(s) 423
BsaAI YACGTR 2 cut(s) 178, 413
BsaJI CCNNGG 2 cut(s) 385, 692
BsaXI ACNNNNNCTCC 2 cut(s) 347, 377
Bsc4I CCNNNNNNNGG 4 cut(s) 167, 254, 346, 648
Bse1I ACTGG 1 cut(s) 831
Bse3DI GCAATG 2 cut(s) 24, 652
BseBI CCWGG 1 cut(s) 321
BseDI CCNNGG 2 cut(s) 385, 692
BseGI GGATG 3 cut(s) 267, 316, 592
BseLI CCNNNNNNNGG 4 cut(s) 167, 254, 346, 648
BseMI GCAATG 2 cut(s) 24, 652
BseMII CTCAG 1 cut(s) 414
BseNI ACTGG 1 cut(s) 831
BseRI GAGGAG 1 cut(s) 368
BshFI GGCC 6 cut(s) 59, 210, 280, 516, 750, 824
BslI CCNNNNNNNGG 4 cut(s) 167, 254, 346, 648
BsmAI GTCTC 3 cut(s) 391, 477, 819
BsmBI CGTCTC 1 cut(s) 391
BsmI GAATGC 1 cut(s) 333
BsnI GGCC 6 cut(s) 59, 210, 280, 516, 750, 824
Bsp143I GATC 5 cut(s) 92, 181, 244, 683, 742
Bsp1720I GCTNAGC 1 cut(s) 423
Bsp19I CCATGG 2 cut(s) 385, 692
BspACI CCGC 3 cut(s) 79, 517, 609
BspANI GGCC 6 cut(s) 59, 210, 280, 516, 750, 824
BspCNI CTCAG 1 cut(s) 415
BspHI TCATGA 1 cut(s) 6
BspLI GGNNCC 4 cut(s) 159, 183, 357, 823
BspPI GGATC 5 cut(s) 100, 176, 189, 239, 691
BsrDI GCAATG 2 cut(s) 24, 652
BsrI ACTGG 1 cut(s) 831
BssECI CCNNGG 2 cut(s) 385, 692
BssMI GATC 5 cut(s) 92, 181, 244, 683, 742
BssSI CACGAG 2 cut(s) 467, 816
BssT1I CCWWGG 2 cut(s) 385, 692
Bst2BI CACGAG 2 cut(s) 467, 816
Bst2UI CCWGG 1 cut(s) 321
Bst4CI ACNGT 4 cut(s) 50, 88, 99, 408
Bst6I CTCTTC 1 cut(s) 345
BstBAI YACGTR 2 cut(s) 178, 413
BstC8I GCNNGC 1 cut(s) 24
BstDEI CTNAG 1 cut(s) 423
BstDSI CCRYGG 2 cut(s) 385, 692
BstF5I GGATG 3 cut(s) 267, 316, 592
BstKTI GATC 5 cut(s) 95, 184, 247, 686, 745
BstMAI GTCTC 3 cut(s) 391, 477, 819
BstMBI GATC 5 cut(s) 92, 181, 244, 683, 742
BstMWI GCNNNNNNNGC 3 cut(s) 23, 513, 608
BstNI CCWGG 1 cut(s) 321
BstNSI RCATGY 2 cut(s) 488, 799
BstSCI CCNGG 1 cut(s) 319
BstSFI CTRYAG 1 cut(s) 84
BstV2I GAAGAC 1 cut(s) 409
BstX2I RGATCY 2 cut(s) 181, 244
BstXI CCANNNNNNTGG 1 cut(s) 308
BstYI RGATCY 2 cut(s) 181, 244
BsuRI GGCC 6 cut(s) 59, 210, 280, 516, 750, 824
BtgI CCRYGG 2 cut(s) 385, 692
BtsCI GGATG 3 cut(s) 267, 316, 592
BtsIMutI CAGTG 2 cut(s) 159, 784
Cac8I GCNNGC 1 cut(s) 24
CciI TCATGA 1 cut(s) 6
Cfr13I GGNCC 4 cut(s) 278, 689, 749, 822
Csp6I GTAC 1 cut(s) 382
CviAII CATG 7 cut(s) 7, 386, 485, 577, 693, 746, 796
CviQI GTAC 1 cut(s) 382
DdeI CTNAG 1 cut(s) 423
DpnI GATC 5 cut(s) 94, 183, 246, 685, 744
DpnII GATC 5 cut(s) 92, 181, 244, 683, 742
EaeI YGGCCR 2 cut(s) 57, 208
Eam1104I CTCTTC 1 cut(s) 345
EarI CTCTTC 1 cut(s) 345
EciI GGCGGA 1 cut(s) 598
Eco130I CCWWGG 2 cut(s) 385, 692
Eco47I GGWCC 1 cut(s) 689
Eco72I CACGTG 2 cut(s) 178, 413
EcoRI GAATTC 1 cut(s) 556
EcoRII CCWGG 1 cut(s) 319
EcoT14I CCWWGG 2 cut(s) 385, 692
EcoT22I ATGCAT 2 cut(s) 318, 490
ErhI CCWWGG 2 cut(s) 385, 692
Esp3I CGTCTC 1 cut(s) 391
FaeI CATG 7 cut(s) 10, 389, 488, 580, 696, 749, 799
FalI AAGNNNNNCTT 2 cut(s) 411, 443
FatI CATG 7 cut(s) 6, 385, 484, 576, 692, 745, 795
Fnu4HI GCNGC 1 cut(s) 517
FokI GGATG 3 cut(s) 274, 303, 599
Fsp4HI GCNGC 1 cut(s) 517
FspBI CTAG 1 cut(s) 39
GluI GCNGC 1 cut(s) 517
HaeIII GGCC 6 cut(s) 59, 210, 280, 516, 750, 824
Hin1II CATG 7 cut(s) 10, 389, 488, 580, 696, 749, 799
HincII GTYRAC 1 cut(s) 267
HindII GTYRAC 1 cut(s) 267
HinfI GANTC 3 cut(s) 268, 470, 659
HphI GGTGA 1 cut(s) 182
Hpy166II GTNNAC 2 cut(s) 173, 267
Hpy188I TCNGA 2 cut(s) 103, 244
Hpy188III TCNNGA 4 cut(s) 7, 467, 474, 700
Hpy8I GTNNAC 2 cut(s) 173, 267
Hpy99I CGWCG 1 cut(s) 151
HpyAV CCTTC 2 cut(s) 489, 777
HpyCH4III ACNGT 4 cut(s) 50, 88, 99, 408
HpyCH4IV ACGT 3 cut(s) 177, 412, 773
HpyCH4V TGCA 5 cut(s) 22, 164, 316, 488, 799
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 513, 608
HpyF3I CTNAG 1 cut(s) 423
HpySE526I ACGT 3 cut(s) 177, 412, 773
Hsp92II CATG 7 cut(s) 10, 389, 488, 580, 696, 749, 799
Kzo9I GATC 5 cut(s) 92, 181, 244, 683, 742
LmnI GCTCC 2 cut(s) 14, 355
LweI GCATC 2 cut(s) 325, 532
MaeI CTAG 1 cut(s) 39
MaeII ACGT 3 cut(s) 177, 412, 773
MaeIII GTNAC 2 cut(s) 408, 779
MalI GATC 5 cut(s) 94, 183, 246, 685, 744
MboI GATC 5 cut(s) 92, 181, 244, 683, 742
MboII GAAGA 3 cut(s) 362, 414, 455
MfeI CAATTG 1 cut(s) 216
MflI RGATCY 2 cut(s) 181, 244
MlsI TGGCCA 2 cut(s) 59, 210
MluCI AATT 5 cut(s) 74, 187, 216, 556, 580
MluNI TGGCCA 2 cut(s) 59, 210
MlyI GAGTC 2 cut(s) 262, 479
MnlI CCTC 5 cut(s) 306, 346, 506, 571, 673
Mox20I TGGCCA 2 cut(s) 59, 210
Mph1103I ATGCAT 2 cut(s) 318, 490
MroXI GAANNNNTTC 1 cut(s) 595
MscI TGGCCA 2 cut(s) 59, 210
MseI TTAA 2 cut(s) 491, 525
MslI CAYNNNNRTG 3 cut(s) 306, 483, 726
Msp20I TGGCCA 2 cut(s) 59, 210
MspR9I CCNGG 1 cut(s) 321
MunI CAATTG 1 cut(s) 216
Mva1269I GAATGC 1 cut(s) 333
MvaI CCWGG 1 cut(s) 321
MwoI GCNNNNNNNGC 3 cut(s) 23, 513, 608
NcoI CCATGG 2 cut(s) 385, 692
NdeII GATC 5 cut(s) 92, 181, 244, 683, 742
NlaIII CATG 7 cut(s) 10, 389, 488, 580, 696, 749, 799
NlaIV GGNNCC 4 cut(s) 159, 183, 357, 823
NmuCI GTSAC 2 cut(s) 408, 779
NsiI ATGCAT 2 cut(s) 318, 490
NspI RCATGY 2 cut(s) 488, 799
PagI TCATGA 1 cut(s) 6
PctI GAATGC 1 cut(s) 333
PdmI GAANNNNTTC 1 cut(s) 595
PfeI GAWTC 1 cut(s) 659
PflMI CCANNNNNTGG 1 cut(s) 254
PkrI GCNGC 1 cut(s) 518
PleI GAGTC 2 cut(s) 262, 478
PmaCI CACGTG 2 cut(s) 178, 413
PmlI CACGTG 2 cut(s) 178, 413
PpsI GAGTC 2 cut(s) 262, 478
Ppu21I YACGTR 2 cut(s) 178, 413
PsiI TTATAA 1 cut(s) 443
Psp6I CCWGG 1 cut(s) 319
PspCI CACGTG 2 cut(s) 178, 413
PspGI CCWGG 1 cut(s) 319
PspN4I GGNNCC 4 cut(s) 159, 183, 357, 823
PspPI GGNCC 4 cut(s) 278, 689, 749, 822
PsuI RGATCY 2 cut(s) 181, 244
RsaI GTAC 1 cut(s) 383
RsaNI GTAC 1 cut(s) 382
RseI CAYNNNNRTG 3 cut(s) 306, 483, 726
SaqAI TTAA 2 cut(s) 491, 525
SatI GCNGC 1 cut(s) 517
Sau3AI GATC 5 cut(s) 92, 181, 244, 683, 742
Sau96I GGNCC 4 cut(s) 278, 689, 749, 822
SchI GAGTC 2 cut(s) 262, 479
ScrFI CCNGG 1 cut(s) 321
SfaNI GCATC 2 cut(s) 325, 532
SfcI CTRYAG 1 cut(s) 84
SinI GGWCC 1 cut(s) 689
SmiMI CAYNNNNRTG 3 cut(s) 306, 483, 726
Sse9I AATT 5 cut(s) 74, 187, 216, 556, 580
SsiI CCGC 3 cut(s) 79, 517, 609
SspMI CTAG 1 cut(s) 39
StyD4I CCNGG 1 cut(s) 319
StyI CCWWGG 2 cut(s) 385, 692
TaaI ACNGT 4 cut(s) 50, 88, 99, 408
TaiI ACGT 3 cut(s) 180, 415, 776
TaqI TCGA 2 cut(s) 451, 475
TasI AATT 5 cut(s) 74, 187, 216, 556, 580
TauI GCSGC 1 cut(s) 519
TfiI GAWTC 1 cut(s) 659
Tru1I TTAA 2 cut(s) 491, 525
Tru9I TTAA 2 cut(s) 491, 525
TscAI CASTG 2 cut(s) 159, 784
TseFI GTSAC 2 cut(s) 408, 779
Tsp45I GTSAC 2 cut(s) 408, 779
TspDTI ATGAA 2 cut(s) 23, 593
TspGWI ACGGA 2 cut(s) 225, 696
TspRI CASTG 2 cut(s) 159, 784
Van91I CCANNNNNTGG 1 cut(s) 254
VpaK11BI GGWCC 1 cut(s) 689
XapI RAATTY 3 cut(s) 187, 556, 580
XceI RCATGY 2 cut(s) 488, 799
XmnI GAANNNNTTC 1 cut(s) 595
XspI CTAG 1 cut(s) 39
Zsp2I ATGCAT 2 cut(s) 318, 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.