Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0007988

Physical Location & Seq

Genomic Coordinates Reverse (-)

1118548 .. 1124831

Gene Structure 6284 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1012400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1086 bp

ATGGAGGCTGTTGTGGTCGATGCCGGCTCCAAGCTCCTGAAGGCAGGACCCGCCATTCCCGATCAAGCTCCTTCCATGATAATTCCTAACCAAATGAAACGCGTGCCTGATGATGGGTCAATGAATGATAATTCATCACTTGAAGATATCTTTGTTGATCCAGTTGTGCGAGGTCTTATTACTGACTGGGATGCCATGGAAGACCTGTTGCATCATGTTCTATATACTGGCCTTGGATGGGAAATGGGAAATGAAGGGCAGATACTATTTACTGATCCACTTTGTACTCCCAAGGCTGTCAGAGAGCAGTTGGTGCAACTGATGTTTGAAACATTTAACATTTCGGGCTTCTATGCATCAGAGCAAGCAGTATTGTCACTTTATGCTGTAGGACGCATCTCGGGATGCACTGTTGATATTGGCCATGGAAAAATAGATATTGCACCAGTCATTGAAGGTGCTGTTCAGCACATTGCCTCAAGGAGGTTTGAAATTGGCGGTACTGATTTGACGAAATTACTTGCTGAAGAGCTTGGGAAATCCAATCCTCTAGTGAATATCAAGATGTCTGATATTGAGAAAATAAAAGAGCAATATTCGTGTTGTGCTGAAGATGAACTTGCTTATGAAAAGACCAAAAATTCATGCCAGATAGAGCAGCATACCCTTCCTGATGGACAGGTTATTACGATTGGAAGAGAAAGATATACTGTTGGTGAGGCTCTATTCCAACCATCCATATTGGGTTTAGAGGCTCATGGAATTGTCGAGCAGCTTGTTCGTTGTATTTCAACAGTATCGTCTGATAATCACCGGCAGTTGCTAGAAAATACTGTACTGTGTGGTGGCACTGCTTCTATGACTGGTTTTGAAGAAAGGTTTCAGAAAGAAGCTGGTCTATGCTCATCGGCTGTTCGTCCTGCTTTAATTAAGCCTCCAGAGTATATGCCAGAGAATTTATCAATGTATTCAGCGTGGGTGGGCGGTGCCATACTTGCCAAAGTTGTGTTCCCACAAAATCAACATGTAACAAAAGCAGACTACGATGAAACTGGACCATCTGTTGTTCATCGGAAATGCTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0005198 GO:0005200 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006996 GO:0007010 GO:0007275 GO:0008150 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009838 GO:0009908 GO:0009987 GO:0010154 GO:0010227 GO:0016043 GO:0022414 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048316 GO:0048367 GO:0048437 GO:0048608 GO:0048731 GO:0048856 GO:0051276 GO:0061458 GO:0071840 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Actin

Protein Analysis

362

Amino Acids

39.56

Weight (kDa)

4.87

Isoelectric Point (pI)

41.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Actin	PF00022	2 - 361	1.1e-68	Actin
FtsA	PF14450	136 - 307	1.7e-07	Cell division protein FtsA

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007988)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G60830
fragaria_vesca	FvH4_7g01150 FvH4_7g01150 FvH4_7g01150 FvH4_7g01150
malus_domestica	MD02G1309300.v1.1 MD07G1012400.v1.1
prunus_persica	Prupe.2G014900_v2.0.a1 Prupe.2G014900_v2.0.a1 Prupe.2G014900_v2.0.a1 Prupe.2G014900_v2.0.a1 Prupe.2G014900_v2.0.a1
pyrus_communis	pycom07g01000
rosa_chinensis	RchiOBHm_Chr1g0316421
rosa_laevigata	RLG00000030675
rosa_multiflora	Rmu_sc0007485.1_g000001 Rmu_sc0008729.1_g000001
rosa_roxburghii	Rroxscaffold_4G00331030
rosa_rugosa	Rorug01G0010300 Rorug01G0010400
rosa_samantha	Rh1AG020200 Rh1BG015400 Rh1DG015400
rosa_wichuraiana	Rw0G021590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	986
AccII	CGCG	1 cut(s)	102
AciI	CCGC	3 cut(s)	51, 498, 984
AclWI	GGATC	2 cut(s)	152, 269
AcoI	YGGCCR	1 cut(s)	421
AcsI	RAATTY	2 cut(s)	640, 955
AcuI	CTGAAG	3 cut(s)	59, 546, 630
AfaI	GTAC	3 cut(s)	286, 502, 837
AfiI	CCNNNNNNNGG	3 cut(s)	113, 238, 483
AflIII	ACRYGT	2 cut(s)	100, 1024
AgsI	TTSAA	6 cut(s)	143, 329, 455, 491, 792, 872
AjuI	GAANNNNNNNTTGG	2 cut(s)	992, 1024
AluBI	AGCT	5 cut(s)	34, 68, 532, 775, 893
AluI	AGCT	5 cut(s)	34, 68, 532, 775, 893
AlwI	GGATC	2 cut(s)	152, 269
Ama87I	CYCGRG	1 cut(s)	400
AoxI	GGCC	2 cut(s)	229, 421
ApeKI	GCWGC	2 cut(s)	658, 772
ApoI	RAATTY	2 cut(s)	640, 955
Asp700I	GAANNNNTTC	2 cut(s)	879, 1079
AspS9I	GGNCC	2 cut(s)	47, 1055
AsuHPI	GGTGA	2 cut(s)	728, 803
AvaI	CYCGRG	1 cut(s)	400
AvaII	GGWCC	2 cut(s)	47, 1055
BalI	TGGCCA	1 cut(s)	423
BanI	GGYRCC	1 cut(s)	986
BarI	GAAGNNNNNNTAC	2 cut(s)	246, 278
BbsI	GAAGAC	1 cut(s)	207
BbvI	GCAGC	2 cut(s)	670, 784
BccI	CCATC	5 cut(s)	107, 231, 668, 742, 1066
BcgI	CGANNNNNNTGC	2 cut(s)	761, 795
BfaI	CTAG	2 cut(s)	551, 824
BfmI	CTRYAG	1 cut(s)	387
BisI	GCNGC	2 cut(s)	659, 773
BlsI	GCNGC	2 cut(s)	660, 774
Bme18I	GGWCC	2 cut(s)	47, 1055
BmeT110I	CYCGRG	1 cut(s)	400
BmgT120I	GGNCC	2 cut(s)	47, 1055
BmiI	GGNNCC	3 cut(s)	28, 49, 988
BmrI	ACTGGG	1 cut(s)	196
BmsI	GCATC	6 cut(s)	10, 181, 220, 365, 395, 405
BmuI	ACTGGG	1 cut(s)	196
BpiI	GAAGAC	1 cut(s)	207
BpmI	CTGGAG	1 cut(s)	921
BpuEI	CTTGAG	1 cut(s)	463
BsaJI	CCNNGG	4 cut(s)	195, 232, 291, 424
Bsc4I	CCNNNNNNNGG	3 cut(s)	113, 238, 483
Bse118I	RCCGGY	2 cut(s)	23, 813
Bse1I	ACTGG	6 cut(s)	161, 191, 232, 446, 868, 1057
Bse3DI	GCAATG	1 cut(s)	471
BseDI	CCNNGG	4 cut(s)	195, 232, 291, 424
BseGI	GGATG	4 cut(s)	196, 242, 410, 734
BseLI	CCNNNNNNNGG	3 cut(s)	113, 238, 483
BseMI	GCAATG	1 cut(s)	471
BseNI	ACTGG	6 cut(s)	161, 191, 232, 446, 868, 1057
BseXI	GCAGC	2 cut(s)	670, 784
Bsh1236I	CGCG	1 cut(s)	102
BshFI	GGCC	2 cut(s)	231, 423
BshNI	GGYRCC	1 cut(s)	986
BsiHKCI	CYCGRG	1 cut(s)	400
BsiSI	CCGG	2 cut(s)	24, 814
BslI	CCNNNNNNNGG	3 cut(s)	113, 238, 483
BsnI	GGCC	2 cut(s)	231, 423
BsoBI	CYCGRG	1 cut(s)	400
Bsp143I	GATC	3 cut(s)	61, 157, 274
Bsp19I	CCATGG	2 cut(s)	195, 424
BspACI	CCGC	3 cut(s)	51, 498, 984
BspANI	GGCC	2 cut(s)	231, 423
BspFNI	CGCG	1 cut(s)	102
BspLI	GGNNCC	3 cut(s)	28, 49, 988
BspPI	GGATC	2 cut(s)	152, 269
BspQI	GCTCTTC	1 cut(s)	522
BspT107I	GGYRCC	1 cut(s)	986
BsrDI	GCAATG	1 cut(s)	471
BsrFI	RCCGGY	2 cut(s)	23, 813
BsrI	ACTGG	6 cut(s)	161, 191, 232, 446, 868, 1057
BssAI	RCCGGY	2 cut(s)	23, 813
BssECI	CCNNGG	4 cut(s)	195, 232, 291, 424
BssMI	GATC	3 cut(s)	61, 157, 274
BssT1I	CCWWGG	4 cut(s)	195, 232, 291, 424
Bst4CI	ACNGT	5 cut(s)	412, 712, 796, 835, 840
Bst6I	CTCTTC	2 cut(s)	522, 691
BstAPI	GCANNNNNTGC	1 cut(s)	313
BstC8I	GCNNGC	3 cut(s)	25, 104, 366
BstDSI	CCRYGG	2 cut(s)	195, 424
BstENI	CCTNNNNNAGG	1 cut(s)	481
BstF5I	GGATG	4 cut(s)	196, 242, 410, 734
BstFNI	CGCG	1 cut(s)	102
BstKTI	GATC	3 cut(s)	64, 160, 277
BstMBI	GATC	3 cut(s)	61, 157, 274
BstMWI	GCNNNNNNNGC	3 cut(s)	50, 313, 995
BstNSI	RCATGY	1 cut(s)	1028
BstSFI	CTRYAG	1 cut(s)	387
BstUI	CGCG	1 cut(s)	102
BstV1I	GCAGC	2 cut(s)	670, 784
BstV2I	GAAGAC	1 cut(s)	207
BsuRI	GGCC	2 cut(s)	231, 423
BtgI	CCRYGG	2 cut(s)	195, 424
BtsCI	GGATG	4 cut(s)	196, 242, 410, 734
BtsI	GCAGTG	1 cut(s)	849
BtsIMutI	CAGTG	2 cut(s)	408, 849
Cac8I	GCNNGC	3 cut(s)	25, 104, 366
Cfr10I	RCCGGY	2 cut(s)	23, 813
Cfr13I	GGNCC	2 cut(s)	47, 1055
CseI	GACGC	1 cut(s)	402
Csp6I	GTAC	3 cut(s)	285, 501, 836
CviAII	CATG	7 cut(s)	76, 196, 215, 425, 645, 758, 1025
CviQI	GTAC	3 cut(s)	285, 501, 836
DpnI	GATC	3 cut(s)	63, 159, 276
DpnII	GATC	3 cut(s)	61, 157, 274
EaeI	YGGCCR	1 cut(s)	421
Eam1104I	CTCTTC	2 cut(s)	522, 691
EarI	CTCTTC	2 cut(s)	522, 691
Eco130I	CCWWGG	4 cut(s)	195, 232, 291, 424
Eco32I	GATATC	1 cut(s)	148
Eco47I	GGWCC	2 cut(s)	47, 1055
Eco57I	CTGAAG	3 cut(s)	59, 546, 630
Eco88I	CYCGRG	1 cut(s)	400
EcoNI	CCTNNNNNAGG	1 cut(s)	481
EcoO109I	RGGNCCY	1 cut(s)	47
EcoRV	GATATC	1 cut(s)	148
EcoT14I	CCWWGG	4 cut(s)	195, 232, 291, 424
EcoT22I	ATGCAT	1 cut(s)	358
ErhI	CCWWGG	4 cut(s)	195, 232, 291, 424
FaeI	CATG	7 cut(s)	79, 199, 218, 428, 648, 761, 1028
FalI	AAGNNNNNCTT	2 cut(s)	603, 635
FatI	CATG	7 cut(s)	75, 195, 214, 424, 644, 757, 1024
FauI	CCCGC	1 cut(s)	58
Fnu4HI	GCNGC	2 cut(s)	659, 773
FokI	GGATG	4 cut(s)	203, 249, 417, 721
Fsp4HI	GCNGC	2 cut(s)	659, 773
FspBI	CTAG	2 cut(s)	551, 824
GluI	GCNGC	2 cut(s)	659, 773
GsuI	CTGGAG	1 cut(s)	921
HaeIII	GGCC	2 cut(s)	231, 423
HapII	CCGG	2 cut(s)	24, 814
HgaI	GACGC	1 cut(s)	402
Hin1II	CATG	7 cut(s)	79, 199, 218, 428, 648, 761, 1028
HpaII	CCGG	2 cut(s)	24, 814
HphI	GGTGA	2 cut(s)	728, 803
Hpy188I	TCNGA	7 cut(s)	302, 361, 571, 805, 885, 1074, 1085
Hpy188III	TCNNGA	6 cut(s)	37, 59, 402, 562, 671, 938
HpyAV	CCTTC	5 cut(s)	34, 81, 248, 449, 677
HpyCH4III	ACNGT	5 cut(s)	412, 712, 796, 835, 840
HpyCH4V	TGCA	5 cut(s)	211, 316, 356, 408, 443
HpyF10VI	GCNNNNNNNGC	3 cut(s)	50, 313, 995
Hsp92II	CATG	7 cut(s)	79, 199, 218, 428, 648, 761, 1028
KroI	GCCGGC	1 cut(s)	23
KroNI	GCCGGC	1 cut(s)	25
Kzo9I	GATC	3 cut(s)	61, 157, 274
LguI	GCTCTTC	1 cut(s)	522
LmnI	GCTCC	3 cut(s)	32, 39, 73
Lsp1109I	GCAGC	2 cut(s)	670, 784
LweI	GCATC	6 cut(s)	10, 181, 220, 365, 395, 405
MaeI	CTAG	2 cut(s)	551, 824
MaeIII	GTNAC	2 cut(s)	375, 1027
MalI	GATC	3 cut(s)	63, 159, 276
MboI	GATC	3 cut(s)	61, 157, 274
MboII	GAAGA	6 cut(s)	155, 212, 539, 623, 708, 884
MlsI	TGGCCA	1 cut(s)	423
MluCI	AATT	8 cut(s)	81, 130, 492, 515, 640, 762, 927, 955
MluI	ACGCGT	1 cut(s)	100
MluNI	TGGCCA	1 cut(s)	423
MmeI	TCCRAC	1 cut(s)	754
MnlI	CCTC	7 cut(s)	164, 477, 487, 558, 712, 745, 945
Mox20I	TGGCCA	1 cut(s)	423
Mph1103I	ATGCAT	1 cut(s)	358
MroNI	GCCGGC	1 cut(s)	23
MroXI	GAANNNNTTC	2 cut(s)	879, 1079
MscI	TGGCCA	1 cut(s)	423
MseI	TTAA	3 cut(s)	336, 926, 930
Msp20I	TGGCCA	1 cut(s)	423
MspI	CCGG	2 cut(s)	24, 814
MvnI	CGCG	1 cut(s)	102
MwoI	GCNNNNNNNGC	3 cut(s)	50, 313, 995
NaeI	GCCGGC	1 cut(s)	25
NcoI	CCATGG	2 cut(s)	195, 424
NdeII	GATC	3 cut(s)	61, 157, 274
NgoMIV	GCCGGC	1 cut(s)	23
NlaIII	CATG	7 cut(s)	79, 199, 218, 428, 648, 761, 1028
NlaIV	GGNNCC	3 cut(s)	28, 49, 988
NmuCI	GTSAC	1 cut(s)	375
NsiI	ATGCAT	1 cut(s)	358
NspI	RCATGY	1 cut(s)	1028
PacI	TTAATTAA	1 cut(s)	930
PciI	ACATGT	1 cut(s)	1024
PciSI	GCTCTTC	1 cut(s)	522
PdiI	GCCGGC	1 cut(s)	25
PdmI	GAANNNNTTC	2 cut(s)	879, 1079
PkrI	GCNGC	2 cut(s)	660, 774
PpuMI	RGGWCCY	1 cut(s)	47
PscI	ACATGT	1 cut(s)	1024
Psp5II	RGGWCCY	1 cut(s)	47
PspN4I	GGNNCC	3 cut(s)	28, 49, 988
PspPI	GGNCC	2 cut(s)	47, 1055
PspPPI	RGGWCCY	1 cut(s)	47
RsaI	GTAC	3 cut(s)	286, 502, 837
RsaNI	GTAC	3 cut(s)	285, 501, 836
SapI	GCTCTTC	1 cut(s)	522
SaqAI	TTAA	3 cut(s)	336, 926, 930
SatI	GCNGC	2 cut(s)	659, 773
Sau3AI	GATC	3 cut(s)	61, 157, 274
Sau96I	GGNCC	2 cut(s)	47, 1055
SfaNI	GCATC	6 cut(s)	10, 181, 220, 365, 395, 405
SfcI	CTRYAG	1 cut(s)	387
SinI	GGWCC	2 cut(s)	47, 1055
SmlI	CTYRAG	1 cut(s)	478
SmoI	CTYRAG	1 cut(s)	478
Sse9I	AATT	8 cut(s)	81, 130, 492, 515, 640, 762, 927, 955
SsiI	CCGC	3 cut(s)	51, 498, 984
SspI	AATATT	1 cut(s)	596
SspMI	CTAG	2 cut(s)	551, 824
StyI	CCWWGG	4 cut(s)	195, 232, 291, 424
TaaI	ACNGT	5 cut(s)	412, 712, 796, 835, 840
TaqI	TCGA	2 cut(s)	18, 768
TasI	AATT	8 cut(s)	81, 130, 492, 515, 640, 762, 927, 955
TatI	WGTACW	2 cut(s)	284, 835
Tru1I	TTAA	3 cut(s)	336, 926, 930
Tru9I	TTAA	3 cut(s)	336, 926, 930
TscAI	CASTG	2 cut(s)	415, 856
TseFI	GTSAC	1 cut(s)	375
TseI	GCWGC	2 cut(s)	658, 772
Tsp45I	GTSAC	1 cut(s)	375
TspDTI	ATGAA	9 cut(s)	110, 123, 137, 267, 630, 633, 642, 1058, 1062
TspRI	CASTG	2 cut(s)	415, 856
VpaK11BI	GGWCC	2 cut(s)	47, 1055
XagI	CCTNNNNNAGG	1 cut(s)	481
XapI	RAATTY	2 cut(s)	640, 955
XceI	RCATGY	1 cut(s)	1028
XmnI	GAANNNNTTC	2 cut(s)	879, 1079
XspI	CTAG	2 cut(s)	551, 824
Zsp2I	ATGCAT	1 cut(s)	358

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.