MD07G1017100.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0008987
Physical Location & Seq
Forward (+)
1488314 .. 1490923
2610 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1017100.v1.1.491

Sequence Viewer

Length: 1092 bp
ATGCAGAACACCGCCGATGAGACCCTGATCCGAAGTTTTCAGCTAGCCTTTTCCCTAAGGAGCATTTCTCTTGGAGGAGGATTGCAGCCATCAAGACGCAGATCCCTCTTTACCTTGGCAACATCGATGATCATCTTCTCAGCTAAAGCCTACAACGTGGTTGCTCTTGCACCTTGTGCTAAAGCAGCACTTACAAATGAAACAGTTGATCCATTTCTACGGTTGGTTGATGATCGCAAATTGCAAGCTGTCAATTCTGGAGCTGACCAAGTAAGGAAAGTTTATGGATCAAAAGAAGATGATGAGGATGCTTTAAGGTCACTTTCTGCTATAGAAAAATCTGAGAGCCAATCTAAGGAATCGTTTACTACCATGATAGTGCAGACGCTTAGAAATTCACCAGATGTAAACTTCTTATCACATTGTGATTATCGGAACCATGATTCATCAATTATAAAACAGCAGTTGCTTAATGATTTTCTGCCTGATGATGCATGTCCATTGGGAGCTCCGTTGAACATGGAAACACCCAGAGAAATAGACCAAGTTGGCGTACAAGATGATGGAGAACCTGATAAGGCGGAGCCCCCATTATTCACAATTGGGGATAATGCCTTACCCAATGCATCTGAAAATCAAACCGATCCTGGCACAAAAGTTGCAATGGAAAGTTTAAGCCTAATAAGCGTTGATCAGCTTTTAGATTCGGTTTTGGAGACGACACATCAAGTTGGAAGATTATCAGTCTCAGCTGCTACAAACATGCCTTACATGGAAATAGCTGGCCAGTGTGAGGCCATTCAAATGGGGAAGCAGCAGAAGTTGTCGACGTTTATAGTTTCCCAACAGAGACAAGAAAGTTTGATTAGATTTTCTAATGACTGTAGCCAGGAAAAGGAGACTCCATCTGTTGTTCCGCTGGGCGCTCCTACGAGTGGAAATCCATTCCTTGAATCGGGTGCTATTTCACTCAACCAATCTGTTGGCAATGGCTCTTTGATGCTTTGCGCAACTGAGTATCAGCCCTATCCGCACTTCCAGCTACCGGCATCAAGCCCCTACGATAACTTTTTAAAGGCTGCAGGTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

364

Amino Acids

39.49

Weight (kDa)

4.69

Isoelectric Point (pI)

46.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 455
Acc16I TGCGCA 1 cut(s) 1009
Acc36I ACCTGC 1 cut(s) 1073
AccI GTMKAC 1 cut(s) 827
AciI CCGC 4 cut(s) 12, 581, 917, 1031
AclWI GGATC 5 cut(s) 22, 96, 203, 295, 638
AcoI YGGCCR 1 cut(s) 784
AcsI RAATTY 1 cut(s) 394
AdeI CACNNNGTG 2 cut(s) 176, 425
AfaI GTAC 1 cut(s) 555
AfiI CCNNNNNNNGG 6 cut(s) 355, 793, 895, 935, 955, 1045
AgsI TTSAA 3 cut(s) 517, 803, 953
AjnI CCWGG 2 cut(s) 646, 888
AluBI AGCT 9 cut(s) 43, 143, 248, 263, 509, 697, 752, 782, 1042
AluI AGCT 9 cut(s) 43, 143, 248, 263, 509, 697, 752, 782, 1042
Alw21I GWGCWC 1 cut(s) 511
Alw26I GTCTC 5 cut(s) 14, 710, 751, 844, 893
AlwI GGATC 5 cut(s) 22, 96, 203, 295, 638
AoxI GGCC 2 cut(s) 784, 795
ApeKI GCWGC 5 cut(s) 85, 185, 752, 814, 1079
ApoI RAATTY 1 cut(s) 394
AspLEI GCGC 2 cut(s) 926, 1010
AsuHPI GGTGA 1 cut(s) 390
AsuNHI GCTAGC 1 cut(s) 43
AxyI CCTNAGG 1 cut(s) 56
BalI TGGCCA 1 cut(s) 786
BanII GRGCYC 2 cut(s) 511, 588
Bbv12I GWGCWC 1 cut(s) 511
BbvI GCAGC 5 cut(s) 97, 197, 739, 826, 1066
BccI CCATC 3 cut(s) 97, 557, 913
BciT130I CCWGG 2 cut(s) 648, 890
BclI TGATCA 2 cut(s) 129, 691
BcoDI GTCTC 5 cut(s) 14, 710, 751, 844, 893
BfaI CTAG 1 cut(s) 44
BfmI CTRYAG 3 cut(s) 330, 883, 1080
BfoI RGCGCY 1 cut(s) 927
BfuAI ACCTGC 1 cut(s) 1073
BisI GCNGC 5 cut(s) 86, 186, 753, 815, 1080
BlsI GCNGC 5 cut(s) 87, 187, 754, 816, 1081
Bme1390I CCNGG 2 cut(s) 648, 890
BmiI GGNNCC 2 cut(s) 437, 585
BmrFI CCNGG 2 cut(s) 648, 890
BmsI GCATC 5 cut(s) 298, 481, 635, 990, 1058
BmtI GCTAGC 1 cut(s) 47
BplI GAGNNNNNCTC 2 cut(s) 52, 84
BpmI CTGGAG 1 cut(s) 279
Bsa29I ATCGAT 1 cut(s) 125
BsaBI GATNNNNATC 1 cut(s) 131
BsaI GGTCTC 1 cut(s) 14
BsaJI CCNNGG 1 cut(s) 114
Bsc4I CCNNNNNNNGG 6 cut(s) 355, 793, 895, 935, 955, 1045
Bse118I RCCGGY 1 cut(s) 1045
Bse1I ACTGG 1 cut(s) 787
Bse21I CCTNAGG 1 cut(s) 56
Bse3DI GCAATG 2 cut(s) 669, 994
Bse8I GATNNNNATC 1 cut(s) 131
BseBI CCWGG 2 cut(s) 648, 890
BseCI ATCGAT 1 cut(s) 125
BseDI CCNNGG 1 cut(s) 114
BseGI GGATG 1 cut(s) 313
BseJI GATNNNNATC 1 cut(s) 131
BseLI CCNNNNNNNGG 6 cut(s) 355, 793, 895, 935, 955, 1045
BseMI GCAATG 2 cut(s) 669, 994
BseMII CTCAG 4 cut(s) 153, 333, 762, 1005
BseNI ACTGG 1 cut(s) 787
BseRI GAGGAG 1 cut(s) 90
BseXI GCAGC 5 cut(s) 97, 197, 739, 826, 1066
BseYI CCCAGC 1 cut(s) 919
BsgI GTGCAG 1 cut(s) 401
BshFI GGCC 2 cut(s) 786, 797
BshVI ATCGAT 1 cut(s) 125
BsiHKAI GWGCWC 1 cut(s) 511
BsiSI CCGG 1 cut(s) 1046
BslI CCNNNNNNNGG 6 cut(s) 355, 793, 895, 935, 955, 1045
BsmAI GTCTC 5 cut(s) 14, 710, 751, 844, 893
BsmBI CGTCTC 1 cut(s) 710
BsnI GGCC 2 cut(s) 786, 797
Bso31I GGTCTC 1 cut(s) 14
Bsp1286I GDGCHC 2 cut(s) 511, 588
Bsp143I GATC 8 cut(s) 27, 101, 129, 208, 232, 287, 643, 691
BspACI CCGC 4 cut(s) 12, 581, 917, 1031
BspANI GGCC 2 cut(s) 786, 797
BspCNI CTCAG 4 cut(s) 152, 334, 761, 1006
BspDI ATCGAT 1 cut(s) 125
BspLI GGNNCC 2 cut(s) 437, 585
BspMAI CTGCAG 1 cut(s) 1084
BspMI ACCTGC 1 cut(s) 1073
BspOI GCTAGC 1 cut(s) 47
BspPI GGATC 5 cut(s) 22, 96, 203, 295, 638
BspTNI GGTCTC 1 cut(s) 14
BsrDI GCAATG 2 cut(s) 669, 994
BsrFI RCCGGY 1 cut(s) 1045
BsrI ACTGG 1 cut(s) 787
BssAI RCCGGY 1 cut(s) 1045
BssECI CCNNGG 1 cut(s) 114
BssMI GATC 8 cut(s) 27, 101, 129, 208, 232, 287, 643, 691
BssT1I CCWWGG 1 cut(s) 114
Bst2UI CCWGG 2 cut(s) 648, 890
Bst4CI ACNGT 3 cut(s) 205, 222, 884
BstAPI GCANNNNNTGC 1 cut(s) 176
BstC8I GCNNGC 3 cut(s) 45, 246, 784
BstDEI CTNAG 7 cut(s) 56, 139, 342, 354, 389, 748, 1014
BstF5I GGATG 1 cut(s) 313
BstH2I RGCGCY 1 cut(s) 927
BstHHI GCGC 2 cut(s) 926, 1010
BstKTI GATC 8 cut(s) 30, 104, 132, 211, 235, 290, 646, 694
BstMAI GTCTC 5 cut(s) 14, 710, 751, 844, 893
BstMBI GATC 8 cut(s) 27, 101, 129, 208, 232, 287, 643, 691
BstMWI GCNNNNNNNGC 5 cut(s) 176, 185, 684, 1030, 1039
BstNI CCWGG 2 cut(s) 648, 890
BstNSI RCATGY 2 cut(s) 498, 766
BstSCI CCNGG 2 cut(s) 646, 888
BstSFI CTRYAG 3 cut(s) 330, 883, 1080
BstV1I GCAGC 5 cut(s) 97, 197, 739, 826, 1066
BstX2I RGATCY 1 cut(s) 101
BstXI CCANNNNNNTGG 2 cut(s) 805, 983
BstYI RGATCY 1 cut(s) 101
Bsu15I ATCGAT 1 cut(s) 125
Bsu36I CCTNAGG 1 cut(s) 56
BsuRI GGCC 2 cut(s) 786, 797
BsuTUI ATCGAT 1 cut(s) 125
BtsCI GGATG 1 cut(s) 313
BtsIMutI CAGTG 1 cut(s) 794
BveI ACCTGC 1 cut(s) 1073
Cac8I GCNNGC 3 cut(s) 45, 246, 784
CfoI GCGC 2 cut(s) 926, 1010
Cfr10I RCCGGY 1 cut(s) 1045
ClaI ATCGAT 1 cut(s) 125
CseI GACGC 2 cut(s) 105, 394
Csp6I GTAC 1 cut(s) 554
CviAII CATG 6 cut(s) 373, 440, 495, 520, 763, 772
CviQI GTAC 1 cut(s) 554
DdeI CTNAG 7 cut(s) 56, 139, 342, 354, 389, 748, 1014
DpnI GATC 8 cut(s) 29, 103, 131, 210, 234, 289, 645, 693
DpnII GATC 8 cut(s) 27, 101, 129, 208, 232, 287, 643, 691
DraI TTTAAA 1 cut(s) 1074
DraIII CACNNNGTG 2 cut(s) 176, 425
EaeI YGGCCR 1 cut(s) 784
EciI GGCGGA 1 cut(s) 596
Ecl136II GAGCTC 1 cut(s) 509
Eco130I CCWWGG 1 cut(s) 114
Eco24I GRGCYC 2 cut(s) 511, 588
Eco31I GGTCTC 1 cut(s) 14
Eco53kI GAGCTC 1 cut(s) 509
Eco81I CCTNAGG 1 cut(s) 56
EcoICRI GAGCTC 1 cut(s) 509
EcoRII CCWGG 2 cut(s) 646, 888
EcoT14I CCWWGG 1 cut(s) 114
EcoT22I ATGCAT 2 cut(s) 496, 628
EcoT38I GRGCYC 2 cut(s) 511, 588
ErhI CCWWGG 1 cut(s) 114
Esp3I CGTCTC 1 cut(s) 710
FaeI CATG 6 cut(s) 376, 443, 498, 523, 766, 775
FalI AAGNNNNNCTT 2 cut(s) 174, 206
FatI CATG 6 cut(s) 372, 439, 494, 519, 762, 771
FbaI TGATCA 2 cut(s) 129, 691
FblI GTMKAC 1 cut(s) 827
Fnu4HI GCNGC 5 cut(s) 86, 186, 753, 815, 1080
FokI GGATG 1 cut(s) 320
FriOI GRGCYC 2 cut(s) 511, 588
Fsp4HI GCNGC 5 cut(s) 86, 186, 753, 815, 1080
FspBI CTAG 1 cut(s) 44
FspI TGCGCA 1 cut(s) 1009
GlaI GCGC 2 cut(s) 925, 1009
GluI GCNGC 5 cut(s) 86, 186, 753, 815, 1080
GsaI CCCAGC 1 cut(s) 923
GsuI CTGGAG 1 cut(s) 279
HaeII RGCGCY 1 cut(s) 927
HaeIII GGCC 2 cut(s) 786, 797
HapII CCGG 1 cut(s) 1046
HgaI GACGC 2 cut(s) 105, 394
HhaI GCGC 2 cut(s) 926, 1010
Hin1II CATG 6 cut(s) 376, 443, 498, 523, 766, 775
Hin6I GCGC 2 cut(s) 924, 1008
HinP1I GCGC 2 cut(s) 924, 1008
HincII GTYRAC 1 cut(s) 828
HindII GTYRAC 1 cut(s) 828
HinfI GANTC 5 cut(s) 359, 443, 704, 901, 953
HpaII CCGG 1 cut(s) 1046
HphI GGTGA 1 cut(s) 390
Hpy166II GTNNAC 3 cut(s) 366, 409, 828
Hpy188I TCNGA 4 cut(s) 32, 343, 435, 631
Hpy188III TCNNGA 2 cut(s) 93, 258
Hpy8I GTNNAC 3 cut(s) 366, 409, 828
Hpy99I CGWCG 1 cut(s) 832
HpyCH4III ACNGT 3 cut(s) 205, 222, 884
HpyCH4IV ACGT 2 cut(s) 156, 830
HpyCH4V TGCA 9 cut(s) 4, 85, 170, 244, 382, 494, 626, 662, 1082
HpyF10VI GCNNNNNNNGC 5 cut(s) 176, 185, 684, 1030, 1039
HpyF3I CTNAG 7 cut(s) 56, 139, 342, 354, 389, 748, 1014
HpySE526I ACGT 2 cut(s) 156, 830
Hsp92II CATG 6 cut(s) 376, 443, 498, 523, 766, 775
HspAI GCGC 2 cut(s) 924, 1008
Ksp22I TGATCA 2 cut(s) 129, 691
Kzo9I GATC 8 cut(s) 27, 101, 129, 208, 232, 287, 643, 691
LmnI GCTCC 6 cut(s) 60, 260, 506, 514, 583, 931
Lsp1109I GCAGC 5 cut(s) 97, 197, 739, 826, 1066
LweI GCATC 5 cut(s) 298, 481, 635, 990, 1058
MaeI CTAG 1 cut(s) 44
MaeII ACGT 2 cut(s) 156, 830
MaeIII GTNAC 1 cut(s) 318
MalI GATC 8 cut(s) 29, 103, 131, 210, 234, 289, 645, 693
MboI GATC 8 cut(s) 27, 101, 129, 208, 232, 287, 643, 691
MboII GAAGA 3 cut(s) 127, 308, 747
MfeI CAATTG 1 cut(s) 600
MflI RGATCY 1 cut(s) 101
MhlI GDGCHC 2 cut(s) 511, 588
MlsI TGGCCA 1 cut(s) 786
MluCI AATT 5 cut(s) 239, 253, 394, 450, 600
MluNI TGGCCA 1 cut(s) 786
MlyI GAGTC 1 cut(s) 895
MmeI TCCRAC 1 cut(s) 712
MnlI CCTC 5 cut(s) 68, 71, 116, 298, 787
Mox20I TGGCCA 1 cut(s) 786
Mph1103I ATGCAT 2 cut(s) 496, 628
MscI TGGCCA 1 cut(s) 786
MseI TTAA 4 cut(s) 314, 471, 674, 1073
MslI CAYNNNNRTG 2 cut(s) 377, 803
Msp20I TGGCCA 1 cut(s) 786
MspA1I CMGCKG 2 cut(s) 752, 919
MspI CCGG 1 cut(s) 1046
MspR9I CCNGG 2 cut(s) 648, 890
MunI CAATTG 1 cut(s) 600
MvaI CCWGG 2 cut(s) 648, 890
MwoI GCNNNNNNNGC 5 cut(s) 176, 185, 684, 1030, 1039
NdeII GATC 8 cut(s) 27, 101, 129, 208, 232, 287, 643, 691
NheI GCTAGC 1 cut(s) 43
NlaIII CATG 6 cut(s) 376, 443, 498, 523, 766, 775
NlaIV GGNNCC 2 cut(s) 437, 585
NmuCI GTSAC 1 cut(s) 318
NsbI TGCGCA 1 cut(s) 1009
NsiI ATGCAT 2 cut(s) 496, 628
NspI RCATGY 2 cut(s) 498, 766
PfeI GAWTC 4 cut(s) 359, 443, 704, 953
PkrI GCNGC 5 cut(s) 87, 187, 754, 816, 1081
PleI GAGTC 1 cut(s) 895
PpsI GAGTC 1 cut(s) 895
PsiI TTATAA 1 cut(s) 455
Psp124BI GAGCTC 1 cut(s) 511
Psp6I CCWGG 2 cut(s) 646, 888
PspFI CCCAGC 1 cut(s) 919
PspGI CCWGG 2 cut(s) 646, 888
PspN4I GGNNCC 2 cut(s) 437, 585
PstI CTGCAG 1 cut(s) 1084
PsuI RGATCY 1 cut(s) 101
PvuII CAGCTG 1 cut(s) 752
RsaI GTAC 1 cut(s) 555
RsaNI GTAC 1 cut(s) 554
RseI CAYNNNNRTG 2 cut(s) 377, 803
SacI GAGCTC 1 cut(s) 511
SalI GTCGAC 1 cut(s) 826
SaqAI TTAA 4 cut(s) 314, 471, 674, 1073
SatI GCNGC 5 cut(s) 86, 186, 753, 815, 1080
Sau3AI GATC 8 cut(s) 27, 101, 129, 208, 232, 287, 643, 691
SchI GAGTC 1 cut(s) 895
ScrFI CCNGG 2 cut(s) 648, 890
SduI GDGCHC 2 cut(s) 511, 588
SfaNI GCATC 5 cut(s) 298, 481, 635, 990, 1058
SfcI CTRYAG 3 cut(s) 330, 883, 1080
SmiMI CAYNNNNRTG 2 cut(s) 377, 803
Sse9I AATT 5 cut(s) 239, 253, 394, 450, 600
SsiI CCGC 4 cut(s) 12, 581, 917, 1031
SspMI CTAG 1 cut(s) 44
SstI GAGCTC 1 cut(s) 511
StyD4I CCNGG 2 cut(s) 646, 888
StyI CCWWGG 1 cut(s) 114
TaaI ACNGT 3 cut(s) 205, 222, 884
TaiI ACGT 2 cut(s) 159, 833
TaqI TCGA 2 cut(s) 125, 827
TasI AATT 5 cut(s) 239, 253, 394, 450, 600
TfiI GAWTC 4 cut(s) 359, 443, 704, 953
Tru1I TTAA 4 cut(s) 314, 471, 674, 1073
Tru9I TTAA 4 cut(s) 314, 471, 674, 1073
TscAI CASTG 1 cut(s) 794
TseFI GTSAC 1 cut(s) 318
TseI GCWGC 5 cut(s) 85, 185, 752, 814, 1079
Tsp45I GTSAC 1 cut(s) 318
TspDTI ATGAA 2 cut(s) 213, 435
TspGWI ACGGA 1 cut(s) 501
TspRI CASTG 1 cut(s) 794
XapI RAATTY 1 cut(s) 394
XceI RCATGY 2 cut(s) 498, 766
XmiI GTMKAC 1 cut(s) 827
XspI CTAG 1 cut(s) 44
Zsp2I ATGCAT 2 cut(s) 496, 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.