pycom07g01100

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0008987
Physical Location & Seq
Forward (+)
900396 .. 904204
3809 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g01100.4

Sequence Viewer

Length: 1881 bp
ATGAAATTACAAGACTATCCTCCGCAGTTGTATTTAGGTCATGCATATTTGTGCCGAAAATATTGGTTTTCATGTACAAGTTGTGATCTGTTTATATATGGTAGGCCATTATTTGCAAGCAGCGTGCTTGGCATTGTACAGATTCTACTTGATCAAACACGACACGATGAAATACGTCTTTTAGGATGCCAAACTCTAATTGAGTTTATCAATAATCAGAAGGATGGTACATACATGTTTAATTTAGATGGCATGATTCCAAAACTTTGTCTTTTAGCTCAAGAAACGAGGGAGGATGGGACGGATAAGTGTTTGCGTTCTGCTGGGCTTCAAGCTCCATCATCAATGGTTTGGTTCATGGGTGAATTCTCTCATATTTCATCAGAATTTGATAGCGTTGTTTCAGTTGTCTTGGAAAATTATGGAGGTCCAAAGAATAAGTCAGAAAAACAAGACACCCAGATTGGGAGTTCAGAAGAAGTTTCACCTTCACTAGAACCCATGACAAGGATTTCATCCTTGAGGCTGCTAGGGAGTGAAATAGGCGAAGTCCATGTATCAGGGGACGACATGAATAACCCCAGATTCTGGTCAAAGGTTTGCCTGCACAAGATTGCTAAGTTAGCCAAGGAAGCTACAACCGTACGGCGTGTATTAGAATCTTTGTTCCGCTATTTTGATAATGGAGATCTCTGGTCTCCTAAACATGGACTCGCGCTGTCTGTCTTGATGGATATGCAGTTAATAATTGAAAATTGTGGGCAGAACAGGCACTTCATGTTGTCCATCTTAATTAAGCATCTTGATCACAAGAATGTTCTTAAGAACCCAAATAAATATGCAGTCCAATCATCAGTTGCGATAATTGGTGCTCTGAGTGATATGATGAGACACCTGCGGAAAAGTATACATTGCTCACTTGATGATTCAAATTTGGGGGCCGAAGTTGGAGATGCAGGTCCTGTTCTTGACATGATGTCCGTGATGTTAGAAAACATGTCTAACATTACCGTTATGGCTAGAACCTTGATTTCTACTGTTTACCGTACTGGTCAAGTTGTGGCGACAATACCTAATTTGACATATCAAAACAAGACATTTCCTGAGGCATTATTTCATCAGTTACTCGTAGCTATGGTCTGCGTAGACCATGAAAGCAGGGTGGGCGCACACCGTATTTTTTCTGTCGTGCTTGTTCCCTCATCTGTTTGCCCTCGTCCTTGTGCTGCTGCGCCCATTACTACAAAGAAAAATAATATTGGAAGGACCCTCTCTAGAACTGTATCAGTATTTTCTTCATCAGCTGCGCTCTTTGAAAAATTAAACCATCAATCTCACTCACAGGAAAACATTGCTCAAGAGATGAAGGACAAAGTTGTAATTGTGGAAGAAGCAAAGGTTCCTAACGAATCCATGTTGAACAGATTGAAGTCCAAATTTAGTTCAAGAAGGCATCCATCAACTACAAGTGAGTCTATCGGTAATGAGGATGGTTCAGTCAGCAATCACTCCGTGATGAACCGGTTGAAGTCAACTTATAGTCGAGCCTACAGTATTAGAAGGAACCCATCAGATACACCTTCTGCTGATGAAAAAACTACTAGTATTTCAGATAAAGATTCGACAATGTCCCTTAGGTTAAGCAGTCGTCAGATTACCCTTCTGCTTTCATCAATCTGGGCACAATCCATCTCTCCTTCAAATACACCTGAGAACTATGAAGCAATTGCTAATACCTACAGTCTTGTGCTACTTTATCCTCGAACTAAGAACACCTCCGATGAGACCCTGATCCGAAGTTTTCAGCTAGCCTTTTCCCTAAGGAGCATTTCTCTTGGAGGAGGTAAGCCCTCGAGTCTACAGACGGTTTCTTTTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

627

Amino Acids

69.74

Weight (kDa)

8.47

Isoelectric Point (pI)

50.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 903
Acc36I ACCTGC 2 cut(s) 903, 947
AccB7I CCANNNNNTGG 1 cut(s) 588
AccI GTMKAC 3 cut(s) 907, 1146, 1858
AccII CGCG 1 cut(s) 716
AciI CCGC 3 cut(s) 23, 670, 898
AclWI GGATC 1 cut(s) 1786
AcsI RAATTY 4 cut(s) 365, 386, 931, 1436
AdeI CACNNNGTG 1 cut(s) 1513
AfaI GTAC 5 cut(s) 76, 138, 229, 645, 1048
AfiI CCNNNNNNNGG 4 cut(s) 465, 507, 588, 707
AflII CTTAAG 1 cut(s) 821
AflIII ACRYGT 2 cut(s) 234, 996
AgeI ACCGGT 1 cut(s) 1521
AgsI TTSAA 9 cut(s) 332, 752, 930, 1316, 1420, 1429, 1446, 1528, 1701
AhdI GACNNNNNGTC 1 cut(s) 976
AhlI ACTAGT 1 cut(s) 1601
AluBI AGCT 6 cut(s) 278, 335, 635, 1133, 1304, 1807
AluI AGCT 6 cut(s) 278, 335, 635, 1133, 1304, 1807
Alw21I GWGCWC 1 cut(s) 874
Alw26I GTCTC 3 cut(s) 702, 883, 1778
AlwI GGATC 1 cut(s) 1786
AlwNI CAGNNNCTG 2 cut(s) 588, 962
Ama87I CYCGRG 1 cut(s) 1852
AoxI GGCC 2 cut(s) 104, 939
ApeKI GCWGC 5 cut(s) 120, 526, 1226, 1229, 1304
ApoI RAATTY 4 cut(s) 365, 386, 931, 1436
ArsI GACNNNNNNTTYG 2 cut(s) 425, 457
AsiGI ACCGGT 1 cut(s) 1521
AspLEI GCGC 4 cut(s) 718, 1169, 1234, 1309
AspS9I GGNCC 4 cut(s) 428, 939, 959, 1266
AsuHPI GGTGA 2 cut(s) 374, 477
AsuNHI GCTAGC 1 cut(s) 1807
AvaI CYCGRG 1 cut(s) 1852
AvaII GGWCC 3 cut(s) 428, 959, 1266
AxyI CCTNAGG 3 cut(s) 1104, 1634, 1820
BaeGI GKGCMC 1 cut(s) 1684
Bbv12I GWGCWC 1 cut(s) 874
BbvI GCAGC 5 cut(s) 132, 513, 1213, 1216, 1291
BceAI ACGGC 1 cut(s) 662
BclI TGATCA 2 cut(s) 151, 805
BcoDI GTCTC 3 cut(s) 702, 883, 1778
BcuI ACTAGT 1 cut(s) 1601
BfaI CTAG 6 cut(s) 494, 530, 1020, 1275, 1602, 1808
BfmI CTRYAG 3 cut(s) 1549, 1738, 1859
BfrI CTTAAG 1 cut(s) 821
BfuAI ACCTGC 2 cut(s) 903, 947
BglII AGATCT 1 cut(s) 688
BisI GCNGC 5 cut(s) 121, 527, 1227, 1230, 1305
BlsI GCNGC 5 cut(s) 122, 528, 1228, 1231, 1306
Bme18I GGWCC 3 cut(s) 428, 959, 1266
BmeRI GACNNNNNGTC 1 cut(s) 976
BmeT110I CYCGRG 1 cut(s) 1852
BmgT120I GGNCC 4 cut(s) 428, 939, 959, 1266
BmiI GGNNCC 4 cut(s) 940, 1268, 1401, 1565
BmsI GCATC 4 cut(s) 176, 808, 943, 1462
BmtI GCTAGC 1 cut(s) 1811
BplI GAGNNNNNCTC 2 cut(s) 1816, 1848
BpuEI CTTGAG 3 cut(s) 264, 541, 1341
BsaI GGTCTC 2 cut(s) 702, 1778
BsaJI CCNNGG 1 cut(s) 627
BsaWI WCCGGW 1 cut(s) 1521
Bsc4I CCNNNNNNNGG 4 cut(s) 465, 507, 588, 707
Bse118I RCCGGY 1 cut(s) 1521
Bse1I ACTGG 1 cut(s) 1054
Bse21I CCTNAGG 3 cut(s) 1104, 1634, 1820
Bse3DI GCAATG 2 cut(s) 910, 1350
BseDI CCNNGG 1 cut(s) 627
BseGI GGATG 6 cut(s) 191, 229, 301, 515, 1453, 1495
BseLI CCNNNNNNNGG 4 cut(s) 465, 507, 588, 707
BseMI GCAATG 2 cut(s) 910, 1350
BseMII CTCAG 3 cut(s) 866, 1095, 1701
BseNI ACTGG 1 cut(s) 1054
BseRI GAGGAG 1 cut(s) 1854
BseSI GKGCMC 1 cut(s) 1684
BseXI GCAGC 5 cut(s) 132, 513, 1213, 1216, 1291
BseYI CCCAGC 1 cut(s) 323
BsgI GTGCAG 1 cut(s) 590
Bsh1236I CGCG 1 cut(s) 716
BshFI GGCC 2 cut(s) 106, 941
BshTI ACCGGT 1 cut(s) 1521
BsiHKAI GWGCWC 1 cut(s) 874
BsiHKCI CYCGRG 1 cut(s) 1852
BsiSI CCGG 1 cut(s) 1522
BsiWI CGTACG 1 cut(s) 643
BslFI GGGAC 3 cut(s) 313, 578, 1615
BslI CCNNNNNNNGG 4 cut(s) 465, 507, 588, 707
BsmAI GTCTC 3 cut(s) 702, 883, 1778
BsmFI GGGAC 3 cut(s) 313, 578, 1615
BsnI GGCC 2 cut(s) 106, 941
Bso31I GGTCTC 2 cut(s) 702, 1778
BsoBI CYCGRG 1 cut(s) 1852
Bsp1286I GDGCHC 2 cut(s) 874, 1684
Bsp1407I TGTACA 2 cut(s) 74, 136
Bsp143I GATC 5 cut(s) 85, 151, 688, 805, 1791
BspACI CCGC 3 cut(s) 23, 670, 898
BspANI GGCC 2 cut(s) 106, 941
BspCNI CTCAG 3 cut(s) 867, 1096, 1702
BspFNI CGCG 1 cut(s) 716
BspLI GGNNCC 4 cut(s) 940, 1268, 1401, 1565
BspMI ACCTGC 2 cut(s) 903, 947
BspOI GCTAGC 1 cut(s) 1811
BspPI GGATC 1 cut(s) 1786
BspTI CTTAAG 1 cut(s) 821
BspTNI GGTCTC 2 cut(s) 702, 1778
BsrDI GCAATG 2 cut(s) 910, 1350
BsrFI RCCGGY 1 cut(s) 1521
BsrGI TGTACA 2 cut(s) 74, 136
BsrI ACTGG 1 cut(s) 1054
BssAI RCCGGY 1 cut(s) 1521
BssECI CCNNGG 1 cut(s) 627
BssMI GATC 5 cut(s) 85, 151, 688, 805, 1791
BssNAI GTATAC 1 cut(s) 908
BssT1I CCWWGG 1 cut(s) 627
Bst1107I GTATAC 1 cut(s) 908
Bst4CI ACNGT 9 cut(s) 643, 1012, 1039, 1046, 1175, 1282, 1553, 1742, 1867
BstAFI CTTAAG 1 cut(s) 821
BstAUI TGTACA 2 cut(s) 74, 136
BstC8I GCNNGC 4 cut(s) 118, 125, 605, 1809
BstDEI CTNAG 7 cut(s) 618, 875, 1104, 1634, 1710, 1767, 1820
BstF5I GGATG 6 cut(s) 191, 229, 301, 515, 1453, 1495
BstFNI CGCG 1 cut(s) 716
BstHHI GCGC 4 cut(s) 718, 1169, 1234, 1309
BstKTI GATC 5 cut(s) 88, 154, 691, 808, 1794
BstMAI GTCTC 3 cut(s) 702, 883, 1778
BstMBI GATC 5 cut(s) 85, 151, 688, 805, 1791
BstMWI GCNNNNNNNGC 5 cut(s) 129, 623, 632, 769, 1164
BstNSI RCATGY 2 cut(s) 238, 1000
BstSFI CTRYAG 3 cut(s) 1549, 1738, 1859
BstSLI GKGCMC 1 cut(s) 1684
BstUI CGCG 1 cut(s) 716
BstV1I GCAGC 5 cut(s) 132, 513, 1213, 1216, 1291
BstX2I RGATCY 1 cut(s) 688
BstYI RGATCY 1 cut(s) 688
BstZ17I GTATAC 1 cut(s) 908
Bsu36I CCTNAGG 3 cut(s) 1104, 1634, 1820
BsuRI GGCC 2 cut(s) 106, 941
BtsCI GGATG 6 cut(s) 191, 229, 301, 515, 1453, 1495
BveI ACCTGC 2 cut(s) 903, 947
Cac8I GCNNGC 4 cut(s) 118, 125, 605, 1809
CaiI CAGNNNCTG 2 cut(s) 588, 962
CfoI GCGC 4 cut(s) 718, 1169, 1234, 1309
Cfr10I RCCGGY 1 cut(s) 1521
Cfr13I GGNCC 4 cut(s) 428, 939, 959, 1266
Csp6I GTAC 5 cut(s) 75, 137, 228, 644, 1047
CspAI ACCGGT 1 cut(s) 1521
CviQI GTAC 5 cut(s) 75, 137, 228, 644, 1047
DdeI CTNAG 7 cut(s) 618, 875, 1104, 1634, 1710, 1767, 1820
DpnI GATC 5 cut(s) 87, 153, 690, 807, 1793
DpnII GATC 5 cut(s) 85, 151, 688, 805, 1791
DraIII CACNNNGTG 1 cut(s) 1513
DriI GACNNNNNGTC 1 cut(s) 976
Eam1105I GACNNNNNGTC 1 cut(s) 976
Eco130I CCWWGG 1 cut(s) 627
Eco31I GGTCTC 2 cut(s) 702, 1778
Eco47I GGWCC 3 cut(s) 428, 959, 1266
Eco81I CCTNAGG 3 cut(s) 1104, 1634, 1820
Eco88I CYCGRG 1 cut(s) 1852
EcoO109I RGGNCCY 2 cut(s) 959, 1266
EcoRI GAATTC 1 cut(s) 365
EcoT14I CCWWGG 1 cut(s) 627
EcoT22I ATGCAT 1 cut(s) 46
ErhI CCWWGG 1 cut(s) 627
FaqI GGGAC 3 cut(s) 313, 578, 1615
FbaI TGATCA 2 cut(s) 151, 805
FblI GTMKAC 3 cut(s) 907, 1146, 1858
Fnu4HI GCNGC 5 cut(s) 121, 527, 1227, 1230, 1305
FokI GGATG 6 cut(s) 198, 236, 308, 502, 1440, 1502
Fsp4HI GCNGC 5 cut(s) 121, 527, 1227, 1230, 1305
FspBI CTAG 6 cut(s) 494, 530, 1020, 1275, 1602, 1808
GlaI GCGC 4 cut(s) 717, 1168, 1233, 1308
GluI GCNGC 5 cut(s) 121, 527, 1227, 1230, 1305
GsaI CCCAGC 1 cut(s) 327
HaeIII GGCC 2 cut(s) 106, 941
HapII CCGG 1 cut(s) 1522
HhaI GCGC 4 cut(s) 718, 1169, 1234, 1309
Hin6I GCGC 4 cut(s) 716, 1167, 1232, 1307
HinP1I GCGC 4 cut(s) 716, 1167, 1232, 1307
HincII GTYRAC 1 cut(s) 1533
HindII GTYRAC 1 cut(s) 1533
HpaII CCGG 1 cut(s) 1522
HphI GGTGA 2 cut(s) 374, 477
Hpy166II GTNNAC 5 cut(s) 908, 1042, 1147, 1533, 1859
Hpy188III TCNNGA 8 cut(s) 281, 727, 803, 968, 1103, 1275, 1358, 1446
Hpy8I GTNNAC 5 cut(s) 908, 1042, 1147, 1533, 1859
HpyAV CCTTC 9 cut(s) 214, 498, 1257, 1360, 1443, 1554, 1590, 1670, 1707
HpyCH4III ACNGT 9 cut(s) 643, 1012, 1039, 1046, 1175, 1282, 1553, 1742, 1867
HpyCH4IV ACGT 1 cut(s) 175
HpyCH4V TGCA 6 cut(s) 44, 116, 607, 739, 842, 956
HpyF10VI GCNNNNNNNGC 5 cut(s) 129, 623, 632, 769, 1164
HpyF3I CTNAG 7 cut(s) 618, 875, 1104, 1634, 1710, 1767, 1820
HpySE526I ACGT 1 cut(s) 175
HspAI GCGC 4 cut(s) 716, 1167, 1232, 1307
Ksp22I TGATCA 2 cut(s) 151, 805
Kzo9I GATC 5 cut(s) 85, 151, 688, 805, 1791
LmnI GCTCC 2 cut(s) 340, 1824
Lsp1109I GCAGC 5 cut(s) 132, 513, 1213, 1216, 1291
LweI GCATC 4 cut(s) 176, 808, 943, 1462
MaeI CTAG 6 cut(s) 494, 530, 1020, 1275, 1602, 1808
MaeII ACGT 1 cut(s) 175
MaeIII GTNAC 1 cut(s) 1122
MalI GATC 5 cut(s) 87, 153, 690, 807, 1793
MboI GATC 5 cut(s) 85, 151, 688, 805, 1791
MboII GAAGA 3 cut(s) 488, 1287, 1400
MfeI CAATTG 1 cut(s) 1725
MflI RGATCY 1 cut(s) 688
MhlI GDGCHC 2 cut(s) 874, 1684
MlyI GAGTC 3 cut(s) 705, 1481, 1864
MmeI TCCRAC 1 cut(s) 928
Mph1103I ATGCAT 1 cut(s) 46
MseI TTAA 7 cut(s) 240, 743, 791, 795, 822, 1322, 1640
MslI CAYNNNNRTG 2 cut(s) 49, 813
MspA1I CMGCKG 1 cut(s) 1304
MspCI CTTAAG 1 cut(s) 821
MspI CCGG 1 cut(s) 1522
MunI CAATTG 1 cut(s) 1725
MvnI CGCG 1 cut(s) 716
MwoI GCNNNNNNNGC 5 cut(s) 129, 623, 632, 769, 1164
NdeII GATC 5 cut(s) 85, 151, 688, 805, 1791
NheI GCTAGC 1 cut(s) 1807
NlaIV GGNNCC 4 cut(s) 940, 1268, 1401, 1565
NsiI ATGCAT 1 cut(s) 46
NspI RCATGY 2 cut(s) 238, 1000
PacI TTAATTAA 1 cut(s) 795
PaeR7I CTCGAG 1 cut(s) 1852
PaqCI CACCTGC 1 cut(s) 903
PciI ACATGT 2 cut(s) 234, 996
PfeI GAWTC 7 cut(s) 142, 256, 585, 659, 926, 1409, 1619
Pfl23II CGTACG 1 cut(s) 643
PflFI GACNNNGTC 1 cut(s) 1627
PflMI CCANNNNNTGG 1 cut(s) 588
PinAI ACCGGT 1 cut(s) 1521
PkrI GCNGC 5 cut(s) 122, 528, 1228, 1231, 1306
PleI GAGTC 3 cut(s) 705, 1480, 1863
PpsI GAGTC 3 cut(s) 705, 1480, 1863
PpuMI RGGWCCY 2 cut(s) 959, 1266
PscI ACATGT 2 cut(s) 234, 996
Psp5II RGGWCCY 2 cut(s) 959, 1266
PspFI CCCAGC 1 cut(s) 323
PspLI CGTACG 1 cut(s) 643
PspN4I GGNNCC 4 cut(s) 940, 1268, 1401, 1565
PspPI GGNCC 4 cut(s) 428, 939, 959, 1266
PspPPI RGGWCCY 2 cut(s) 959, 1266
PspXI VCTCGAGB 1 cut(s) 1852
PstNI CAGNNNCTG 2 cut(s) 588, 962
PsuI RGATCY 1 cut(s) 688
PsyI GACNNNGTC 1 cut(s) 1627
PvuII CAGCTG 1 cut(s) 1304
RsaI GTAC 5 cut(s) 76, 138, 229, 645, 1048
RsaNI GTAC 5 cut(s) 75, 137, 228, 644, 1047
RseI CAYNNNNRTG 2 cut(s) 49, 813
SaqAI TTAA 7 cut(s) 240, 743, 791, 795, 822, 1322, 1640
SatI GCNGC 5 cut(s) 121, 527, 1227, 1230, 1305
Sau3AI GATC 5 cut(s) 85, 151, 688, 805, 1791
Sau96I GGNCC 4 cut(s) 428, 939, 959, 1266
SchI GAGTC 3 cut(s) 705, 1481, 1864
SduI GDGCHC 2 cut(s) 874, 1684
SfaNI GCATC 4 cut(s) 176, 808, 943, 1462
SfcI CTRYAG 3 cut(s) 1549, 1738, 1859
Sfr274I CTCGAG 1 cut(s) 1852
SinI GGWCC 3 cut(s) 428, 959, 1266
SlaI CTCGAG 1 cut(s) 1852
SmiMI CAYNNNNRTG 2 cut(s) 49, 813
SmlI CTYRAG 5 cut(s) 279, 520, 821, 1356, 1852
SmoI CTYRAG 5 cut(s) 279, 520, 821, 1356, 1852
SpeI ACTAGT 1 cut(s) 1601
SsiI CCGC 3 cut(s) 23, 670, 898
SspI AATATT 2 cut(s) 62, 1258
SspMI CTAG 6 cut(s) 494, 530, 1020, 1275, 1602, 1808
StyI CCWWGG 1 cut(s) 627
TaaI ACNGT 9 cut(s) 643, 1012, 1039, 1046, 1175, 1282, 1553, 1742, 1867
TaiI ACGT 1 cut(s) 178
TaqI TCGA 4 cut(s) 1543, 1622, 1762, 1853
TatI WGTACW 2 cut(s) 74, 136
TfiI GAWTC 7 cut(s) 142, 256, 585, 659, 926, 1409, 1619
Tru1I TTAA 7 cut(s) 240, 743, 791, 795, 822, 1322, 1640
Tru9I TTAA 7 cut(s) 240, 743, 791, 795, 822, 1322, 1640
TseI GCWGC 5 cut(s) 120, 526, 1226, 1229, 1304
TspGWI ACGGA 3 cut(s) 317, 970, 1501
Tth111I GACNNNGTC 1 cut(s) 1627
Van91I CCANNNNNTGG 1 cut(s) 588
Vha464I CTTAAG 1 cut(s) 821
VpaK11BI GGWCC 3 cut(s) 428, 959, 1266
XapI RAATTY 4 cut(s) 365, 386, 931, 1436
XbaI TCTAGA 1 cut(s) 1274
XceI RCATGY 2 cut(s) 238, 1000
XhoI CTCGAG 1 cut(s) 1852
XmiI GTMKAC 3 cut(s) 907, 1146, 1858
XspI CTAG 6 cut(s) 494, 530, 1020, 1275, 1602, 1808
Zsp2I ATGCAT 1 cut(s) 46
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.