Prupe.2G016800_v2.0.a1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0008987
Physical Location & Seq
Forward (+)
1581613 .. 1593341
11729 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G016800.2

Sequence Viewer

Length: 3162 bp
ATGGGTGTGATTTCAAGGCAAGTGTTCCCTGTTTGTGAAAGTCTCTGTTTCTTTTGCCCTGCATTGCGTGCGAGATCACGGCATCCCGTCAAACGATACAAGAAGCTGCTAGCCGAGATATTTCCTCGGTCTCCGGATGAAGAACCAAATGATCGTAAGATCAGTAAACTATGTGAATATGCCTCAAAAACTCCTCTTCGTATCCCAAAGATCACAACTACTCTGGAGCAAAGGTGTTACAAGGATTTGAGAACTGAGAACTTTCACTCTGTGAAAGTTGTTATGTGTATCTACCGGAAGTTGTTGGTCTCTTGTAAGGAGCAAATGCCATTGTTTGCAAGTAGCGTGCTTAGCATTGTACAAATTCTACTTGATCAATCACGGCATGATGACATACGTATTTTAGGATGCCAAACTCTTTTCGAGTTTGTTAATAATCAGAAGGATGGTACATACATGTTTAACTTAGATGGCATGATTCCAAAACTTTGTCTCTTAGCTCAAGAAATGAGGGAGGATGGGTCGGAAAAGCATTTGCGTTCTGCTGGGCTTAAAACTCTTTCATCAATGGTCTGGTTCATGGGTGAATTCTCTCACATTTCATCAGATTTTGATAATGTTGTTTCAGTTGTCTTGGAAAATTATGGAGGTCCAAAGAATAAGTCAGATGCTAGTATACATGATAAGCAAGACACCCAGAATGGGAGTTCAGAAGAAGCTTCTTCTTCAGGAGAACCCATGACAAGTATTTTGTCTTGGAGGCTGCTAGTGAGTGAAAAAGGCGAAGTCAATGTCTCAGGGGAGGACATGAATAACCCCAGATTTTGGTCAAGGGTTTGCATGCATAACATTGCCAAGTTAGCTAAGGAAGCTACAACCGTACGGCGTGTATTAGAATCTTTGTTCCGCTATTTTGATAATGGCAATCTCTGGTCTCCTAAACATGGGCTCGCATTGTGTGTCTTGATGGATATGCAGTTAATAATTGAAAATTGTGGGCAGAACCGGCACTTTATATTGTCCATCTTAATTAAGCATCTTGATCACAAGAATGTTCTTAAAAACCCAAATATGCAGCTGGACATTGTTGATGTTGCCACCTCTCTTACTCGAGAAGCAAAGGTTCAATCATCAGTTGCTATAATTGGTGCATTGAGTGATATGATGAGACACCTGCGCAAAAGTATACATTGCTCACTTGATGATTCAAATTTGGGGACCGAAGTCATTGAATGGAACCGAAATTTTCGAGCCGTAGTGGATGAATGCCTTGTGCAGTTAACACATAAGGTAGGAGATGCAGGTCCTGTTCTTGACATGATGGCTGTGATGTTAGAAAATATGTCTAACATTACTGTTATGTCTCGAACCTTGATTTCTGCTGTTTACCGTACTGGTCAAATTGTGGCAACAATACCTAATTTGACATATCAAAACAAGACCTTTCCTGAGGCGTTATTTCATCAGTTACTCGTAGCTATGGTCTGTGCGGACCATGAAACCAGGGTGGGTGCACACCGTATATTTTCTGTCGTTCTTGTTCCATCATCTGTTTGCCCTCGTCCTTGTGCTGCTACTCCCCACACTGCAAAAAGAAATGATATAGGAAGGACACTCTCGAGAACTGTGTCTGTATTTTCTTCATCAGCTGCACTCTTTGAAAAATTAAACGAGCGATCCGCCTCACAAGAAAACATTTGTGAAGAGATGAAGGACAAAGCTGTAATTGAAGAAGAAGCAAAGGTTACTAATGAATCGATGTTGAACAGATTGAAGTCCAAATTTAGCTCAAGAAGGCATCAATCAGCTACTAGTGACTCTGTAGGTAATGAGGATTCTGCAATCAATTCTCATTCTGTGATGAACCGGTTGAAGTCAACTTACAGCCGAGCCTACAGTATGAAAAGGAATCCATCAAATACGACTGTTGATGAAAAACCTAGGAGTATTTCACATAAAGAATCGACAATGTCACTTAGGTTAAGCAGTCGCCAGATTACCCTTCTGCTTTCATCAATCTGGGTGCAATCCATCTCTCCTTTAAATACGCCTGATAATTATGAAGCAATTGCTCATACCTACAGCCTTGTGTTGCTATATGCCCGAACTAAGAACACCTCTGACGAGACTCTAATCCGAAGTTTTCAGCTAGCCTTTTCCTTAAGGAGCATATCTCTTGGAGGAGGATTGCAGCCATCAAGGCGCAGATCTCTCTTTACCTTGGCAACATCAATGATCATCTTCTCAGCTAAAGCCTACAACATTGTTGCTCTTGCTCCTTGTGCTAAAGTAGCACTTACAAATGAAACGATTGATCCATTTCTACGGTTGGTTGACGATAGCAAGTTGCAAGCTGTCAATTCTGGACCTGACCAAGTAAGGGAAGTTTATGGATCAAAGGAAGATAACGAGGATGCTTTAAGGTCACTTTCTGCCATAGAAATATCTGAGAGCCAATCTAAGGAATCGTTTGCTACTATGATAGTGCAGACACTGGGAAATTCACCAAATGAGTCATCAACTATAAGACAGCAGTTGCTTAATGATTTTCTGCCTGATGATGCATGTCCGTTGGGAACTCAGTTGTGCATGGAAACACCCATACAAATTTACCAATTTGGCATAGAAGATAATGGAACTCGTGATATGGTTGAGCCCCCATTATTCACAATTGAGGATGATGTCTTACCCAATGCAACTGAAAGTCAAACAGATCCTGACAAAAAAGTTTCCATGGAAAGTCTGAGCCTCATAAGTGTTGATGAGCTTTTAGATTCGGTTTTGGAGACAACACATCAAGTTGGAAGATTATCAGTCTCAACTGCTACAGATATGCCTTATTTGGAAATGGCTGGCCTTTGTGAGGCCCTTCAAATGGGGAAGCAGCAGAGACTGTCCACTTTCGTGGCTGCCCAACTGAGACAAGAAAGTTTGATGAGATTTTCTCCTCATGATTGCAACCAGCAAAAGGAAACTCCCTCTGTTGTTCTGCTGGGTGCTCCGACGAGTGGAAATCCATTCCTTGATTCAAATGCTATTTCACTCAACCAATCTGTTGGCAACGGGCAAACGCTTTGGGCAACTGAATACCAGCATTATCCCCGATTTCAGCTACCTGCATCAAGCCCGTATGATAACTTTTTAAAGGCTGCTGGTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1054

Amino Acids

117.65

Weight (kDa)

6.37

Isoelectric Point (pI)

50.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1182
Acc16I TGCGCA 1 cut(s) 1178
Acc36I ACCTGC 3 cut(s) 1182, 1292, 3124
AccB7I CCANNNNNTGG 1 cut(s) 825
AccI GTMKAC 2 cut(s) 676, 1186
AccIII TCCGGA 1 cut(s) 133
AciI CCGC 3 cut(s) 907, 1490, 1680
AclWI GGATC 4 cut(s) 1671, 2309, 2401, 2708
AcsI RAATTY 7 cut(s) 363, 587, 1210, 1243, 1781, 2500, 2607
AcuI CTGAAG 1 cut(s) 711
AdeI CACNNNGTG 1 cut(s) 271
AfaI GTAC 4 cut(s) 360, 451, 882, 1393
AfiI CCNNNNNNNGG 9 cut(s) 702, 825, 944, 2380, 2461, 2863, 2875, 2968, 3008
AflII CTTAAG 1 cut(s) 2161
AflIII ACRYGT 1 cut(s) 456
AgeI ACCGGT 1 cut(s) 1866
AhlI ACTAGT 1 cut(s) 1811
AjnI CCWGG 1 cut(s) 1502
AleI CACNNNNGTG 1 cut(s) 2903
Alw21I GWGCWC 2 cut(s) 1516, 3001
Alw44I GTGCAC 1 cut(s) 1512
AlwI GGATC 4 cut(s) 1671, 2309, 2401, 2708
AlwNI CAGNNNCTG 4 cut(s) 1307, 2494, 2717, 2893
Ama87I CYCGRG 2 cut(s) 1110, 1618
Aor13HI TCCGGA 1 cut(s) 133
AoxI GGCC 2 cut(s) 2854, 2865
ApaLI GTGCAC 1 cut(s) 1512
ApeKI GCWGC 9 cut(s) 106, 763, 1075, 1571, 1649, 2191, 2884, 2909, 3149
ApoI RAATTY 7 cut(s) 363, 587, 1210, 1243, 1781, 2500, 2607
ArsI GACNNNNNNTTYG 6 cut(s) 647, 679, 733, 765, 777, 809
AsiGI ACCGGT 1 cut(s) 1866
AspA2I CCTAGG 1 cut(s) 1940
AspLEI GCGC 2 cut(s) 1179, 2205
AspS9I GGNCC 6 cut(s) 650, 1218, 1304, 1492, 2366, 2866
AsuHPI GGTGA 2 cut(s) 596, 2496
AsuNHI GCTAGC 2 cut(s) 109, 2149
AvaI CYCGRG 2 cut(s) 1110, 1618
AvaII GGWCC 5 cut(s) 650, 1218, 1304, 1492, 2366
AvrII CCTAGG 1 cut(s) 1940
AxyI CCTNAGG 1 cut(s) 1449
BaeGI GKGCMC 1 cut(s) 1516
BanII GRGCYC 2 cut(s) 951, 2658
BarI GAAGNNNNNNTAC 2 cut(s) 1865, 1897
BauI CACGAG 1 cut(s) 2640
Bbv12I GWGCWC 2 cut(s) 1516, 3001
BbvI GCAGC 9 cut(s) 93, 750, 1087, 1558, 1636, 2203, 2896, 2896, 3136
BceAI ACGGC 4 cut(s) 95, 398, 899, 1238
BciT130I CCWGG 1 cut(s) 1504
BciVI GTATCC 1 cut(s) 212
BclI TGATCA 3 cut(s) 373, 1042, 2235
BcuI ACTAGT 1 cut(s) 1811
BfaI CTAG 6 cut(s) 110, 672, 767, 1812, 1941, 2150
BfmI CTRYAG 4 cut(s) 1821, 1894, 2080, 2826
BfrI CTTAAG 1 cut(s) 2161
BfuAI ACCTGC 3 cut(s) 1182, 1292, 3124
BfuI GTATCC 1 cut(s) 212
BglI GCCNNNNNGGC 1 cut(s) 2200
BglII AGATCT 1 cut(s) 2207
BisI GCNGC 9 cut(s) 107, 764, 1076, 1572, 1650, 2192, 2885, 2910, 3150
BlnI CCTAGG 1 cut(s) 1940
BlpI GCTNAGC 1 cut(s) 350
BlsI GCNGC 9 cut(s) 108, 765, 1077, 1573, 1651, 2193, 2886, 2911, 3151
Bme1390I CCNGG 1 cut(s) 1504
Bme18I GGWCC 5 cut(s) 650, 1218, 1304, 1492, 2366
BmeT110I CYCGRG 2 cut(s) 1110, 1618
BmgT120I GGNCC 6 cut(s) 650, 1218, 1304, 1492, 2366, 2866
BmiI GGNNCC 2 cut(s) 1219, 1238
BmrFI CCNGG 1 cut(s) 1504
BmrI ACTGGG 1 cut(s) 2504
BmsI GCATC 9 cut(s) 91, 398, 658, 1045, 1288, 1807, 2404, 2551, 3128
BmtI GCTAGC 2 cut(s) 113, 2153
BmuI ACTGGG 1 cut(s) 2504
BoxI GACNNNNGTC 1 cut(s) 1223
BplI GAGNNNNNCTC 4 cut(s) 2158, 2190, 2929, 2961
BpmI CTGGAG 1 cut(s) 245
Bpu10I CCTNAGC 1 cut(s) 864
Bpu1102I GCTNAGC 1 cut(s) 350
BpuEI CTTGAG 2 cut(s) 486, 1774
Bsa29I ATCGAT 1 cut(s) 1757
BsaAI YACGTR 1 cut(s) 398
BsaI GGTCTC 3 cut(s) 135, 313, 939
BsaJI CCNNGG 5 cut(s) 125, 1503, 1940, 2220, 2733
BsaWI WCCGGW 3 cut(s) 133, 294, 1866
BsaXI ACNNNNNCTCC 4 cut(s) 218, 248, 506, 536
Bsc4I CCNNNNNNNGG 9 cut(s) 702, 825, 944, 2380, 2461, 2863, 2875, 2968, 3008
Bse118I RCCGGY 2 cut(s) 1005, 1866
Bse1I ACTGG 2 cut(s) 1399, 2499
Bse21I CCTNAGG 1 cut(s) 1449
Bse3DI GCAATG 3 cut(s) 62, 849, 1189
BseAI TCCGGA 1 cut(s) 133
BseBI CCWGG 1 cut(s) 1504
BseCI ATCGAT 1 cut(s) 1757
BseDI CCNNGG 5 cut(s) 125, 1503, 1940, 2220, 2733
BseGI GGATG 8 cut(s) 82, 142, 413, 451, 523, 1267, 2419, 2683
BseLI CCNNNNNNNGG 9 cut(s) 702, 825, 944, 2380, 2461, 2863, 2875, 2968, 3008
BseMI GCAATG 3 cut(s) 62, 849, 1189
BseMII CTCAG 8 cut(s) 246, 810, 1440, 2259, 2439, 2594, 2735, 2909
BseNI ACTGG 2 cut(s) 1399, 2499
BseRI GAGGAG 3 cut(s) 183, 2196, 2937
BseSI GKGCMC 1 cut(s) 1516
BseXI GCAGC 9 cut(s) 93, 750, 1087, 1558, 1636, 2203, 2896, 2896, 3136
BseYI CCCAGC 2 cut(s) 545, 2992
BsgI GTGCAG 3 cut(s) 1295, 1635, 2507
BshFI GGCC 2 cut(s) 2856, 2867
BshTI ACCGGT 1 cut(s) 1866
BshVI ATCGAT 1 cut(s) 1757
BsiHKAI GWGCWC 2 cut(s) 1516, 3001
BsiHKCI CYCGRG 2 cut(s) 1110, 1618
BsiSI CCGG 4 cut(s) 134, 295, 1006, 1867
BsiWI CGTACG 1 cut(s) 880
BslFI GGGAC 1 cut(s) 1231
BslI CCNNNNNNNGG 9 cut(s) 702, 825, 944, 2380, 2461, 2863, 2875, 2968, 3008
BsmFI GGGAC 1 cut(s) 1231
BsmI GAATGC 1 cut(s) 1271
BsnI GGCC 2 cut(s) 2856, 2867
Bso31I GGTCTC 3 cut(s) 135, 313, 939
BsoBI CYCGRG 2 cut(s) 1110, 1618
Bsp1286I GDGCHC 4 cut(s) 951, 1516, 2658, 3001
Bsp13I TCCGGA 1 cut(s) 133
Bsp1407I TGTACA 1 cut(s) 358
Bsp1720I GCTNAGC 1 cut(s) 350
Bsp19I CCATGG 1 cut(s) 2733
BspACI CCGC 3 cut(s) 907, 1490, 1680
BspANI GGCC 2 cut(s) 2856, 2867
BspCNI CTCAG 8 cut(s) 247, 809, 1441, 2258, 2440, 2593, 2736, 2910
BspDI ATCGAT 1 cut(s) 1757
BspEI TCCGGA 1 cut(s) 133
BspHI TCATGA 1 cut(s) 2950
BspLI GGNNCC 2 cut(s) 1219, 1238
BspMI ACCTGC 3 cut(s) 1182, 1292, 3124
BspOI GCTAGC 2 cut(s) 113, 2153
BspPI GGATC 4 cut(s) 1671, 2309, 2401, 2708
BspTI CTTAAG 1 cut(s) 2161
BspTNI GGTCTC 3 cut(s) 135, 313, 939
BsrDI GCAATG 3 cut(s) 62, 849, 1189
BsrFI RCCGGY 2 cut(s) 1005, 1866
BsrGI TGTACA 1 cut(s) 358
BsrI ACTGG 2 cut(s) 1399, 2499
BssAI RCCGGY 2 cut(s) 1005, 1866
BssECI CCNNGG 5 cut(s) 125, 1503, 1940, 2220, 2733
BssNAI GTATAC 2 cut(s) 677, 1187
BssSI CACGAG 1 cut(s) 2640
BssT1I CCWWGG 3 cut(s) 1940, 2220, 2733
Bst1107I GTATAC 2 cut(s) 677, 1187
Bst2BI CACGAG 1 cut(s) 2640
Bst2UI CCWGG 1 cut(s) 1504
Bst4CI ACNGT 9 cut(s) 880, 1357, 1391, 1520, 1627, 1898, 1927, 2328, 2895
Bst6I CTCTTC 2 cut(s) 201, 1698
BstAFI CTTAAG 1 cut(s) 2161
BstAPI GCANNNNNTGC 1 cut(s) 68
BstAUI TGTACA 1 cut(s) 358
BstBAI YACGTR 1 cut(s) 398
BstC8I GCNNGC 8 cut(s) 69, 111, 347, 842, 951, 2151, 2352, 2854
BstDSI CCRYGG 1 cut(s) 2733
BstENI CCTNNNNNAGG 1 cut(s) 2861
BstF5I GGATG 8 cut(s) 82, 142, 413, 451, 523, 1267, 2419, 2683
BstHHI GCGC 2 cut(s) 1179, 2205
BstMWI GCNNNNNNNGC 8 cut(s) 68, 351, 860, 869, 1006, 2200, 2282, 2291
BstNI CCWGG 1 cut(s) 1504
BstNSI RCATGY 3 cut(s) 460, 844, 2568
BstPAI GACNNNNGTC 1 cut(s) 1223
BstSCI CCNGG 1 cut(s) 1502
BstSFI CTRYAG 4 cut(s) 1821, 1894, 2080, 2826
BstSLI GKGCMC 1 cut(s) 1516
BstSNI TACGTA 1 cut(s) 398
BstV1I GCAGC 9 cut(s) 93, 750, 1087, 1558, 1636, 2203, 2896, 2896, 3136
BstX2I RGATCY 2 cut(s) 2207, 2713
BstXI CCANNNNNNTGG 2 cut(s) 2905, 3056
BstYI RGATCY 2 cut(s) 2207, 2713
BstZ17I GTATAC 2 cut(s) 677, 1187
Bsu15I ATCGAT 1 cut(s) 1757
Bsu36I CCTNAGG 1 cut(s) 1449
BsuI GTATCC 1 cut(s) 212
BsuRI GGCC 2 cut(s) 2856, 2867
BsuTUI ATCGAT 1 cut(s) 1757
BtgI CCRYGG 1 cut(s) 2733
BtsCI GGATG 8 cut(s) 82, 142, 413, 451, 523, 1267, 2419, 2683
BtsI GCAGTG 1 cut(s) 1584
BtsIMutI CAGTG 2 cut(s) 1584, 2492
BveI ACCTGC 3 cut(s) 1182, 1292, 3124
Cac8I GCNNGC 8 cut(s) 69, 111, 347, 842, 951, 2151, 2352, 2854
CaiI CAGNNNCTG 4 cut(s) 1307, 2494, 2717, 2893
CciI TCATGA 1 cut(s) 2950
CfoI GCGC 2 cut(s) 1179, 2205
Cfr10I RCCGGY 2 cut(s) 1005, 1866
Cfr13I GGNCC 6 cut(s) 650, 1218, 1304, 1492, 2366, 2866
ClaI ATCGAT 1 cut(s) 1757
Csp6I GTAC 4 cut(s) 359, 450, 881, 1392
CspAI ACCGGT 1 cut(s) 1866
CviQI GTAC 4 cut(s) 359, 450, 881, 1392
DraI TTTAAA 2 cut(s) 2043, 3144
DraIII CACNNNGTG 1 cut(s) 271
Eam1104I CTCTTC 2 cut(s) 201, 1698
EarI CTCTTC 2 cut(s) 201, 1698
EciI GGCGGA 1 cut(s) 1669
Eco105I TACGTA 1 cut(s) 398
Eco130I CCWWGG 3 cut(s) 1940, 2220, 2733
Eco24I GRGCYC 2 cut(s) 951, 2658
Eco31I GGTCTC 3 cut(s) 135, 313, 939
Eco47I GGWCC 5 cut(s) 650, 1218, 1304, 1492, 2366
Eco57I CTGAAG 1 cut(s) 711
Eco81I CCTNAGG 1 cut(s) 1449
Eco88I CYCGRG 2 cut(s) 1110, 1618
EcoNI CCTNNNNNAGG 1 cut(s) 2861
EcoO109I RGGNCCY 2 cut(s) 1304, 2866
EcoRI GAATTC 1 cut(s) 587
EcoRII CCWGG 1 cut(s) 1502
EcoT14I CCWWGG 3 cut(s) 1940, 2220, 2733
EcoT22I ATGCAT 2 cut(s) 846, 2566
EcoT38I GRGCYC 2 cut(s) 951, 2658
ErhI CCWWGG 3 cut(s) 1940, 2220, 2733
FalI AAGNNNNNCTT 2 cut(s) 2280, 2312
FaqI GGGAC 1 cut(s) 1231
FbaI TGATCA 3 cut(s) 373, 1042, 2235
FblI GTMKAC 2 cut(s) 676, 1186
Fnu4HI GCNGC 9 cut(s) 107, 764, 1076, 1572, 1650, 2192, 2885, 2910, 3150
FokI GGATG 8 cut(s) 69, 149, 420, 458, 530, 1274, 2426, 2690
FriOI GRGCYC 2 cut(s) 951, 2658
Fsp4HI GCNGC 9 cut(s) 107, 764, 1076, 1572, 1650, 2192, 2885, 2910, 3150
FspBI CTAG 6 cut(s) 110, 672, 767, 1812, 1941, 2150
FspI TGCGCA 1 cut(s) 1178
GlaI GCGC 2 cut(s) 1178, 2204
GluI GCNGC 9 cut(s) 107, 764, 1076, 1572, 1650, 2192, 2885, 2910, 3150
GsaI CCCAGC 2 cut(s) 549, 2996
GsuI CTGGAG 1 cut(s) 245
HaeIII GGCC 2 cut(s) 2856, 2867
HapII CCGG 4 cut(s) 134, 295, 1006, 1867
HhaI GCGC 2 cut(s) 1179, 2205
Hin6I GCGC 2 cut(s) 1177, 2203
HinP1I GCGC 2 cut(s) 1177, 2203
HincII GTYRAC 3 cut(s) 1281, 1878, 2335
HindII GTYRAC 3 cut(s) 1281, 1878, 2335
HindIII AAGCTT 1 cut(s) 717
HpaI GTTAAC 1 cut(s) 1281
HpaII CCGG 4 cut(s) 134, 295, 1006, 1867
HphI GGTGA 2 cut(s) 596, 2496
Hpy166II GTNNAC 9 cut(s) 167, 677, 1187, 1281, 1387, 1514, 1878, 2335, 2898
Hpy8I GTNNAC 9 cut(s) 167, 677, 1187, 1281, 1387, 1514, 1878, 2335, 2898
Hpy99I CGWCG 1 cut(s) 3007
HpyAV CCTTC 6 cut(s) 436, 1602, 1705, 1788, 2012, 2879
HpyCH4III ACNGT 9 cut(s) 880, 1357, 1391, 1520, 1627, 1898, 1927, 2328, 2895
HpyCH4IV ACGT 1 cut(s) 397
HpyF10VI GCNNNNNNNGC 8 cut(s) 68, 351, 860, 869, 1006, 2200, 2282, 2291
HpySE526I ACGT 1 cut(s) 397
HspAI GCGC 2 cut(s) 1177, 2203
Kpn2I TCCGGA 1 cut(s) 133
Ksp22I TGATCA 3 cut(s) 373, 1042, 2235
KspAI GTTAAC 1 cut(s) 1281
LmnI GCTCC 5 cut(s) 226, 319, 2166, 2281, 3004
Lsp1109I GCAGC 9 cut(s) 93, 750, 1087, 1558, 1636, 2203, 2896, 2896, 3136
LweI GCATC 9 cut(s) 91, 398, 658, 1045, 1288, 1807, 2404, 2551, 3128
MaeI CTAG 6 cut(s) 110, 672, 767, 1812, 1941, 2150
MaeII ACGT 1 cut(s) 397
MaeIII GTNAC 6 cut(s) 236, 1467, 1744, 1814, 1971, 2424
MfeI CAATTG 2 cut(s) 2067, 2670
MflI RGATCY 2 cut(s) 2207, 2713
MhlI GDGCHC 4 cut(s) 951, 1516, 2658, 3001
MlyI GAGTC 3 cut(s) 1811, 2122, 2522
MmeI TCCRAC 3 cut(s) 504, 2782, 3026
Mph1103I ATGCAT 2 cut(s) 846, 2566
MroI TCCGGA 1 cut(s) 133
MslI CAYNNNNRTG 2 cut(s) 1050, 2903
MspA1I CMGCKG 2 cut(s) 1078, 1649
MspCI CTTAAG 1 cut(s) 2161
MspI CCGG 4 cut(s) 134, 295, 1006, 1867
MspR9I CCNGG 1 cut(s) 1504
MunI CAATTG 2 cut(s) 2067, 2670
Mva1269I GAATGC 1 cut(s) 1271
MvaI CCWGG 1 cut(s) 1504
MwoI GCNNNNNNNGC 8 cut(s) 68, 351, 860, 869, 1006, 2200, 2282, 2291
NcoI CCATGG 1 cut(s) 2733
NheI GCTAGC 2 cut(s) 109, 2149
NlaIV GGNNCC 2 cut(s) 1219, 1238
NmeAIII GCCGAG 2 cut(s) 139, 1913
NmuCI GTSAC 3 cut(s) 1814, 1971, 2424
NsbI TGCGCA 1 cut(s) 1178
NsiI ATGCAT 2 cut(s) 846, 2566
NspI RCATGY 3 cut(s) 460, 844, 2568
OliI CACNNNNGTG 1 cut(s) 2903
PacI TTAATTAA 1 cut(s) 1032
PaeI GCATGC 1 cut(s) 844
PaeR7I CTCGAG 2 cut(s) 1110, 1618
PagI TCATGA 1 cut(s) 2950
PaqCI CACCTGC 1 cut(s) 1182
PciI ACATGT 1 cut(s) 456
PctI GAATGC 1 cut(s) 1271
Pfl23II CGTACG 1 cut(s) 880
PflFI GACNNNGTC 1 cut(s) 1969
PflMI CCANNNNNTGG 1 cut(s) 825
PinAI ACCGGT 1 cut(s) 1866
PkrI GCNGC 9 cut(s) 108, 765, 1077, 1573, 1651, 2193, 2886, 2911, 3151
PleI GAGTC 3 cut(s) 1811, 2122, 2521
PpsI GAGTC 3 cut(s) 1811, 2122, 2521
Ppu21I YACGTR 1 cut(s) 398
PpuMI RGGWCCY 1 cut(s) 1304
PscI ACATGT 1 cut(s) 456
PshAI GACNNNNGTC 1 cut(s) 1223
Psp5II RGGWCCY 1 cut(s) 1304
Psp6I CCWGG 1 cut(s) 1502
PspFI CCCAGC 2 cut(s) 545, 2992
PspGI CCWGG 1 cut(s) 1502
PspLI CGTACG 1 cut(s) 880
PspN4I GGNNCC 2 cut(s) 1219, 1238
PspPI GGNCC 6 cut(s) 650, 1218, 1304, 1492, 2366, 2866
PspPPI RGGWCCY 1 cut(s) 1304
PstNI CAGNNNCTG 4 cut(s) 1307, 2494, 2717, 2893
PsuI RGATCY 2 cut(s) 2207, 2713
PsyI GACNNNGTC 1 cut(s) 1969
PvuII CAGCTG 2 cut(s) 1078, 1649
RsaI GTAC 4 cut(s) 360, 451, 882, 1393
RsaNI GTAC 4 cut(s) 359, 450, 881, 1392
RseI CAYNNNNRTG 2 cut(s) 1050, 2903
SatI GCNGC 9 cut(s) 107, 764, 1076, 1572, 1650, 2192, 2885, 2910, 3150
Sau96I GGNCC 6 cut(s) 650, 1218, 1304, 1492, 2366, 2866
SchI GAGTC 3 cut(s) 1811, 2122, 2522
ScrFI CCNGG 1 cut(s) 1504
SduI GDGCHC 4 cut(s) 951, 1516, 2658, 3001
SfaNI GCATC 9 cut(s) 91, 398, 658, 1045, 1288, 1807, 2404, 2551, 3128
SfcI CTRYAG 4 cut(s) 1821, 1894, 2080, 2826
Sfr274I CTCGAG 2 cut(s) 1110, 1618
SinI GGWCC 5 cut(s) 650, 1218, 1304, 1492, 2366
SlaI CTCGAG 2 cut(s) 1110, 1618
SmiMI CAYNNNNRTG 2 cut(s) 1050, 2903
SmlI CTYRAG 5 cut(s) 501, 1110, 1618, 1789, 2161
SmoI CTYRAG 5 cut(s) 501, 1110, 1618, 1789, 2161
SnaBI TACGTA 1 cut(s) 398
SpeI ACTAGT 1 cut(s) 1811
SphI GCATGC 1 cut(s) 844
SsiI CCGC 3 cut(s) 907, 1490, 1680
SspMI CTAG 6 cut(s) 110, 672, 767, 1812, 1941, 2150
StyD4I CCNGG 1 cut(s) 1502
StyI CCWWGG 3 cut(s) 1940, 2220, 2733
TaaI ACNGT 9 cut(s) 880, 1357, 1391, 1520, 1627, 1898, 1927, 2328, 2895
TaiI ACGT 1 cut(s) 400
TaqI TCGA 7 cut(s) 423, 1111, 1249, 1366, 1619, 1757, 1964
TaqII GACCGA 2 cut(s) 117, 1235
TatI WGTACW 1 cut(s) 358
TscAI CASTG 2 cut(s) 1591, 2499
TseFI GTSAC 3 cut(s) 1814, 1971, 2424
TseI GCWGC 9 cut(s) 106, 763, 1075, 1571, 1649, 2191, 2884, 2909, 3149
Tsp45I GTSAC 3 cut(s) 1814, 1971, 2424
TspGWI ACGGA 1 cut(s) 2559
TspRI CASTG 2 cut(s) 1591, 2499
Tth111I GACNNNGTC 1 cut(s) 1969
Van91I CCANNNNNTGG 1 cut(s) 825
Vha464I CTTAAG 1 cut(s) 2161
VneI GTGCAC 1 cut(s) 1512
VpaK11BI GGWCC 5 cut(s) 650, 1218, 1304, 1492, 2366
XagI CCTNNNNNAGG 1 cut(s) 2861
XapI RAATTY 7 cut(s) 363, 587, 1210, 1243, 1781, 2500, 2607
XceI RCATGY 3 cut(s) 460, 844, 2568
XhoI CTCGAG 2 cut(s) 1110, 1618
XmaJI CCTAGG 1 cut(s) 1940
XmiI GTMKAC 2 cut(s) 676, 1186
XspI CTAG 6 cut(s) 110, 672, 767, 1812, 1941, 2150
Zsp2I ATGCAT 2 cut(s) 846, 2566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.