Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0010216

Physical Location & Seq

Genomic Coordinates Reverse (-)

1856762 .. 1860954

Gene Structure 4193 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1022000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1059 bp

ATGGCGACAAGCACCACGCCTTCGTCCCTCATGCTAACGTACGCCTCCTCTTACGCGCACCCACATGACCTCACTCCCTCTCTCGTCTCCTTCCAGCCCAAGCCCCTCTTCAAAACCTTCTGCCTCTGCCCGCTGGCTCTAAGGCCTCGAGAGAGAGAACCAAGCAAATGGTCCAATTCCGAGCCTCACCGAGTGGCCGCGGCGGCTCTGACGGCGGAGGCAGAGGTGGAGGAGGATGTGGACGAGAAGGAAGGAGTGAAAGGAGGTGATGGGTCTGTTGCTGTTGCTGTTTCTGTTCCTTCAAAGCCCAAGAAAGGGAAGGCTGCATTGCTTTTGAAGAGAGACAGAACAAGGTCCAAAAGATTCTTGGAAATTCAGAAACTGAGGGAAACCAAAAAAGAGTATGAGCTGCAGCCAGCACTCGCTTTACTGAAAGAAATGACGAGTACGAAATTTGTAGAAACTGCTGAAGCCCATTTCCGCCTCAACATTGATCCAAAGTATAATGACCAACAGCTAAGGGCAACTGTAAACCTGCCCAAGGGAACTGGACAGACTGTTAAAGTGGCTGTTCTTACTCAAGGTGAAAGGTTTGACGAAGCAAAAAATGCTGGAGCAGATTTGGTTGGTGGAGATGATTTGATACAACAGATAAAAGAAGGATTCATGGAATTTGACAAGCTAATTGCTTCGCCGGATATGATGCCTAAGGTTGCTAGCCTAGGAAAGATTCTAGGACCACGAGGGCTCATGCCAAATCCGAAAGCTGGTACTGTCACGCCTAATATACCACAGGCCATAGCAGAATTCAAGCAGGGGAAAGTTGAATATAGAGCAGATAAAACTGGTATAGTTCATTTGCGGTTTGGAAAAGCCGACTTTCCTGAAGAAGACCTTCTCGTGAACTTGCTAGCAGCAGTTAAATCGGTAGAAGCAAATAAACCACCAGGTGCAAAAGGTGTTTACTGGAAGAGTGCACATATATGCTCGTCAATGGGGCCTTCCATCCGGTTAAATATAAGGGCGATGCTCGATTTCAAGCTTCCAACAAATGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000470 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005840 GO:0006139 GO:0006364 GO:0006396 GO:0006412 GO:0006518 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009526 GO:0009532 GO:0009534 GO:0009535 GO:0009536 GO:0009570 GO:0009579 GO:0009941 GO:0009987 GO:0010467 GO:0015934 GO:0016020 GO:0016070 GO:0016072 GO:0019538 GO:0022613 GO:0022625 GO:0022626 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0032991 GO:0034357 GO:0034470 GO:0034641 GO:0034645 GO:0034660 GO:0042254 GO:0042273 GO:0042651 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043603 GO:0043604 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046483 GO:0055035 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03010 map03010

Pfam Domains

Protein Families

Ribosomal_L1

Protein Analysis

353

Amino Acids

38.52

Weight (kDa)

9.25

Isoelectric Point (pI)

32.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ribosomal_L1	PF00687	144 - 334	7e-62	Ribosomal protein L1p/L10e family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010216)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G63490 AT3G63490
fragaria_vesca	FvH4_7g01650 FvH4_7g01650
malus_domestica	MD02G1302000.v1.1 MD02G1302200.v1.1 MD07G1022000.v1.1
prunus_persica	Prupe.2G022100_v2.0.a1
pyrus_communis	pycom02g25420 pycom07g01670
rosa_chinensis	RchiOBHm_Chr1g0317921
rosa_laevigata	RLG00000030576
rosa_multiflora	Rmu_co8493655.1_g000001 Rmu_ssc0000008.1_g000047
rosa_roxburghii	Rroxscaffold_4G00330120
rosa_rugosa	Rorug01G0018600
rosa_samantha	Rh1AG030200 Rh1BG026300 Rh1CG028800 Rh1DG041300
rosa_wichuraiana	Rw1G002280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	543
AccII	CGCG	2 cut(s)	56, 200
AciI	CCGC	7 cut(s)	131, 198, 200, 203, 215, 481, 862
AclWI	GGATC	1 cut(s)	488
AcoI	YGGCCR	1 cut(s)	195
AcsI	RAATTY	4 cut(s)	372, 452, 671, 806
AcuI	CTGAAG	2 cut(s)	489, 906
AdeI	CACNNNGTG	2 cut(s)	193, 950
AfaI	GTAC	3 cut(s)	41, 448, 772
AfiI	CCNNNNNNNGG	4 cut(s)	314, 315, 541, 767
AgsI	TTSAA	6 cut(s)	112, 303, 337, 811, 827, 1039
AjnI	CCWGG	1 cut(s)	946
AjuI	GAANNNNNNNTTGG	2 cut(s)	92, 124
AluBI	AGCT	5 cut(s)	409, 517, 682, 767, 1042
AluI	AGCT	5 cut(s)	409, 517, 682, 767, 1042
Alw21I	GWGCWC	1 cut(s)	979
Alw26I	GTCTC	2 cut(s)	91, 336
Alw44I	GTGCAC	1 cut(s)	975
AlwI	GGATC	1 cut(s)	488
AlwNI	CAGNNNCTG	1 cut(s)	382
Ama87I	CYCGRG	1 cut(s)	147
AoxI	GGCC	4 cut(s)	143, 195, 795, 998
ApaLI	GTGCAC	1 cut(s)	975
ApeKI	GCWGC	4 cut(s)	323, 409, 412, 914
ApoI	RAATTY	4 cut(s)	372, 452, 671, 806
Asp700I	GAANNNNTTC	1 cut(s)	894
AspA2I	CCTAGG	1 cut(s)	721
AspLEI	GCGC	1 cut(s)	58
AspS9I	GGNCC	4 cut(s)	171, 354, 737, 998
AsuHPI	GGTGA	3 cut(s)	179, 278, 596
AsuNHI	GCTAGC	2 cut(s)	716, 910
AvaI	CYCGRG	1 cut(s)	147
AvaII	GGWCC	3 cut(s)	171, 354, 737
AvrII	CCTAGG	1 cut(s)	721
AxyI	CCTNAGG	1 cut(s)	708
BaeGI	GKGCMC	1 cut(s)	979
BanII	GRGCYC	1 cut(s)	750
BauI	CACGAG	2 cut(s)	741, 899
BbsI	GAAGAC	1 cut(s)	897
Bbv12I	GWGCWC	1 cut(s)	979
BbvI	GCAGC	4 cut(s)	310, 396, 424, 926
BccI	CCATC	2 cut(s)	263, 1013
BceAI	ACGGC	1 cut(s)	228
BcgI	CGANNNNNNTGC	2 cut(s)	906, 940
BciT130I	CCWGG	1 cut(s)	948
BcoDI	GTCTC	2 cut(s)	91, 336
BfaI	CTAG	4 cut(s)	717, 722, 734, 911
BfmI	CTRYAG	1 cut(s)	410
BfuAI	ACCTGC	1 cut(s)	543
BglI	GCCNNNNNGGC	1 cut(s)	203
BisI	GCNGC	7 cut(s)	198, 201, 204, 324, 410, 413, 915
BlnI	CCTAGG	1 cut(s)	721
BlsI	GCNGC	7 cut(s)	199, 202, 205, 325, 411, 414, 916
Bme1390I	CCNGG	1 cut(s)	948
Bme18I	GGWCC	3 cut(s)	171, 354, 737
BmeT110I	CYCGRG	1 cut(s)	147
BmgT120I	GGNCC	4 cut(s)	171, 354, 737, 998
BmiI	GGNNCC	1 cut(s)	999
BmrFI	CCNGG	1 cut(s)	948
BmsI	GCATC	2 cut(s)	693, 1017
BmtI	GCTAGC	2 cut(s)	720, 914
BpiI	GAAGAC	1 cut(s)	897
BpmI	CTGGAG	1 cut(s)	633
Bpu10I	CCTNAGC	1 cut(s)	518
BpuEI	CTTGAG	1 cut(s)	564
BsaJI	CCNNGG	3 cut(s)	198, 540, 721
BsaWI	WCCGGW	1 cut(s)	1008
Bsc4I	CCNNNNNNNGG	4 cut(s)	314, 315, 541, 767
Bse1I	ACTGG	3 cut(s)	553, 850, 971
Bse21I	CCTNAGG	1 cut(s)	708
Bse3DI	GCAATG	1 cut(s)	326
BseBI	CCWGG	1 cut(s)	948
BseDI	CCNNGG	3 cut(s)	198, 540, 721
BseGI	GGATG	2 cut(s)	241, 1005
BseLI	CCNNNNNNNGG	4 cut(s)	314, 315, 541, 767
BseMI	GCAATG	1 cut(s)	326
BseMII	CTCAG	1 cut(s)	374
BseNI	ACTGG	3 cut(s)	553, 850, 971
BseRI	GAGGAG	2 cut(s)	37, 245
BseSI	GKGCMC	1 cut(s)	979
BseXI	GCAGC	4 cut(s)	310, 396, 424, 926
Bsh1236I	CGCG	2 cut(s)	56, 200
BshFI	GGCC	4 cut(s)	145, 197, 797, 1000
BsiHKAI	GWGCWC	1 cut(s)	979
BsiHKCI	CYCGRG	1 cut(s)	147
BsiSI	CCGG	2 cut(s)	695, 1009
BsiWI	CGTACG	1 cut(s)	39
BslFI	GGGAC	1 cut(s)	10
BslI	CCNNNNNNNGG	4 cut(s)	314, 315, 541, 767
BsmAI	GTCTC	2 cut(s)	91, 336
BsmBI	CGTCTC	1 cut(s)	91
BsmFI	GGGAC	1 cut(s)	10
BsnI	GGCC	4 cut(s)	145, 197, 797, 1000
BsoBI	CYCGRG	1 cut(s)	147
Bsp1286I	GDGCHC	2 cut(s)	750, 979
Bsp143I	GATC	1 cut(s)	493
BspACI	CCGC	7 cut(s)	131, 198, 200, 203, 215, 481, 862
BspANI	GGCC	4 cut(s)	145, 197, 797, 1000
BspCNI	CTCAG	1 cut(s)	375
BspFNI	CGCG	2 cut(s)	56, 200
BspLI	GGNNCC	1 cut(s)	999
BspMAI	CTGCAG	1 cut(s)	414
BspMI	ACCTGC	1 cut(s)	543
BspOI	GCTAGC	2 cut(s)	720, 914
BspPI	GGATC	1 cut(s)	488
BsrDI	GCAATG	1 cut(s)	326
BsrI	ACTGG	3 cut(s)	553, 850, 971
BssECI	CCNNGG	3 cut(s)	198, 540, 721
BssMI	GATC	1 cut(s)	493
BssSI	CACGAG	2 cut(s)	741, 899
BssT1I	CCWWGG	2 cut(s)	540, 721
Bst2BI	CACGAG	2 cut(s)	741, 899
Bst2UI	CCWGG	1 cut(s)	948
Bst4CI	ACNGT	3 cut(s)	529, 559, 775
Bst6I	CTCTTC	3 cut(s)	113, 332, 965
BstAPI	GCANNNNNTGC	1 cut(s)	608
BstC8I	GCNNGC	5 cut(s)	131, 135, 417, 718, 912
BstDEI	CTNAG	4 cut(s)	140, 383, 518, 708
BstDSI	CCRYGG	1 cut(s)	198
BstENI	CCTNNNNNAGG	1 cut(s)	539
BstF5I	GGATG	2 cut(s)	241, 1005
BstFNI	CGCG	2 cut(s)	56, 200
BstHHI	GCGC	1 cut(s)	58
BstKTI	GATC	1 cut(s)	496
BstMAI	GTCTC	2 cut(s)	91, 336
BstMBI	GATC	1 cut(s)	493
BstMWI	GCNNNNNNNGC	3 cut(s)	203, 212, 608
BstNI	CCWGG	1 cut(s)	948
BstSCI	CCNGG	1 cut(s)	946
BstSFI	CTRYAG	1 cut(s)	410
BstSLI	GKGCMC	1 cut(s)	979
BstUI	CGCG	2 cut(s)	56, 200
BstV1I	GCAGC	4 cut(s)	310, 396, 424, 926
BstV2I	GAAGAC	1 cut(s)	897
BstXI	CCANNNNNNTGG	1 cut(s)	168
Bsu36I	CCTNAGG	1 cut(s)	708
BsuRI	GGCC	4 cut(s)	145, 197, 797, 1000
BtgI	CCRYGG	1 cut(s)	198
BtgZI	GCGATG	1 cut(s)	1040
BtsCI	GGATG	2 cut(s)	241, 1005
BveI	ACCTGC	1 cut(s)	543
Cac8I	GCNNGC	5 cut(s)	131, 135, 417, 718, 912
CaiI	CAGNNNCTG	1 cut(s)	382
CfoI	GCGC	1 cut(s)	58
Cfr13I	GGNCC	4 cut(s)	171, 354, 737, 998
Cfr42I	CCGCGG	1 cut(s)	201
CsiI	ACCWGGT	1 cut(s)	946
Csp6I	GTAC	3 cut(s)	40, 447, 771
CviAII	CATG	4 cut(s)	31, 65, 667, 751
CviQI	GTAC	3 cut(s)	40, 447, 771
DdeI	CTNAG	4 cut(s)	140, 383, 518, 708
DpnI	GATC	1 cut(s)	495
DpnII	GATC	1 cut(s)	493
DraIII	CACNNNGTG	2 cut(s)	193, 950
EaeI	YGGCCR	1 cut(s)	195
Eam1104I	CTCTTC	3 cut(s)	113, 332, 965
EarI	CTCTTC	3 cut(s)	113, 332, 965
EciI	GGCGGA	2 cut(s)	230, 470
Eco130I	CCWWGG	2 cut(s)	540, 721
Eco147I	AGGCCT	1 cut(s)	145
Eco24I	GRGCYC	1 cut(s)	750
Eco47I	GGWCC	3 cut(s)	171, 354, 737
Eco57I	CTGAAG	2 cut(s)	489, 906
Eco81I	CCTNAGG	1 cut(s)	708
Eco88I	CYCGRG	1 cut(s)	147
EcoNI	CCTNNNNNAGG	1 cut(s)	539
EcoO109I	RGGNCCY	1 cut(s)	998
EcoRI	GAATTC	1 cut(s)	806
EcoRII	CCWGG	1 cut(s)	946
EcoT14I	CCWWGG	2 cut(s)	540, 721
EcoT38I	GRGCYC	1 cut(s)	750
ErhI	CCWWGG	2 cut(s)	540, 721
Esp3I	CGTCTC	1 cut(s)	91
FaeI	CATG	4 cut(s)	34, 68, 670, 754
FalI	AAGNNNNNCTT	4 cut(s)	92, 124, 879, 911
FaqI	GGGAC	1 cut(s)	10
FatI	CATG	4 cut(s)	30, 64, 666, 750
FauI	CCCGC	1 cut(s)	138
Fnu4HI	GCNGC	7 cut(s)	198, 201, 204, 324, 410, 413, 915
FokI	GGATG	2 cut(s)	248, 992
FriOI	GRGCYC	1 cut(s)	750
Fsp4HI	GCNGC	7 cut(s)	198, 201, 204, 324, 410, 413, 915
FspBI	CTAG	4 cut(s)	717, 722, 734, 911
GlaI	GCGC	1 cut(s)	57
GluI	GCNGC	7 cut(s)	198, 201, 204, 324, 410, 413, 915
GsuI	CTGGAG	1 cut(s)	633
HaeIII	GGCC	4 cut(s)	145, 197, 797, 1000
HapII	CCGG	2 cut(s)	695, 1009
HhaI	GCGC	1 cut(s)	58
Hin1II	CATG	4 cut(s)	34, 68, 670, 754
Hin6I	GCGC	1 cut(s)	56
HinP1I	GCGC	1 cut(s)	56
HindIII	AAGCTT	1 cut(s)	1040
HinfI	GANTC	3 cut(s)	363, 663, 730
HpaII	CCGG	2 cut(s)	695, 1009
HphI	GGTGA	3 cut(s)	179, 278, 596
Hpy166II	GTNNAC	5 cut(s)	241, 532, 904, 964, 977
Hpy188I	TCNGA	4 cut(s)	181, 210, 378, 762
Hpy188III	TCNNGA	3 cut(s)	149, 884, 901
Hpy8I	GTNNAC	5 cut(s)	241, 532, 904, 964, 977
HpyCH4III	ACNGT	3 cut(s)	529, 559, 775
HpyCH4IV	ACGT	1 cut(s)	38
HpyCH4V	TGCA	4 cut(s)	326, 412, 953, 977
HpyF10VI	GCNNNNNNNGC	3 cut(s)	203, 212, 608
HpyF3I	CTNAG	4 cut(s)	140, 383, 518, 708
HpySE526I	ACGT	1 cut(s)	38
Hsp92II	CATG	4 cut(s)	34, 68, 670, 754
HspAI	GCGC	1 cut(s)	56
KspI	CCGCGG	1 cut(s)	201
Kzo9I	GATC	1 cut(s)	493
LmnI	GCTCC	1 cut(s)	614
Lsp1109I	GCAGC	4 cut(s)	310, 396, 424, 926
LweI	GCATC	2 cut(s)	693, 1017
MabI	ACCWGGT	1 cut(s)	946
MaeI	CTAG	4 cut(s)	717, 722, 734, 911
MaeII	ACGT	1 cut(s)	38
MaeIII	GTNAC	1 cut(s)	775
MalI	GATC	1 cut(s)	495
MboI	GATC	1 cut(s)	493
MboII	GAAGA	5 cut(s)	100, 349, 899, 902, 982
MhlI	GDGCHC	2 cut(s)	750, 979
MluCI	AATT	6 cut(s)	175, 372, 452, 671, 684, 806
MroXI	GAANNNNTTC	1 cut(s)	894
MseI	TTAA	3 cut(s)	561, 921, 1013
MslI	CAYNNNNRTG	2 cut(s)	63, 982
MspA1I	CMGCKG	2 cut(s)	133, 200
MspI	CCGG	2 cut(s)	695, 1009
MspR9I	CCNGG	1 cut(s)	948
MvaI	CCWGG	1 cut(s)	948
MvnI	CGCG	2 cut(s)	56, 200
MwoI	GCNNNNNNNGC	3 cut(s)	203, 212, 608
NdeII	GATC	1 cut(s)	493
NheI	GCTAGC	2 cut(s)	716, 910
NlaIII	CATG	4 cut(s)	34, 68, 670, 754
NlaIV	GGNNCC	1 cut(s)	999
NmuCI	GTSAC	1 cut(s)	775
PaeR7I	CTCGAG	1 cut(s)	147
PceI	AGGCCT	1 cut(s)	145
PdmI	GAANNNNTTC	1 cut(s)	894
PfeI	GAWTC	3 cut(s)	363, 663, 730
Pfl23II	CGTACG	1 cut(s)	39
PkrI	GCNGC	7 cut(s)	199, 202, 205, 325, 411, 414, 916
Psp6I	CCWGG	1 cut(s)	946
PspGI	CCWGG	1 cut(s)	946
PspLI	CGTACG	1 cut(s)	39
PspN4I	GGNNCC	1 cut(s)	999
PspPI	GGNCC	4 cut(s)	171, 354, 737, 998
PstI	CTGCAG	1 cut(s)	414
PstNI	CAGNNNCTG	1 cut(s)	382
RsaI	GTAC	3 cut(s)	41, 448, 772
RsaNI	GTAC	3 cut(s)	40, 447, 771
RseI	CAYNNNNRTG	2 cut(s)	63, 982
SacII	CCGCGG	1 cut(s)	201
SaqAI	TTAA	3 cut(s)	561, 921, 1013
SatI	GCNGC	7 cut(s)	198, 201, 204, 324, 410, 413, 915
Sau3AI	GATC	1 cut(s)	493
Sau96I	GGNCC	4 cut(s)	171, 354, 737, 998
ScrFI	CCNGG	1 cut(s)	948
SduI	GDGCHC	2 cut(s)	750, 979
SexAI	ACCWGGT	1 cut(s)	946
SfaNI	GCATC	2 cut(s)	693, 1017
SfcI	CTRYAG	1 cut(s)	410
Sfr274I	CTCGAG	1 cut(s)	147
Sfr303I	CCGCGG	1 cut(s)	201
SgrBI	CCGCGG	1 cut(s)	201
SinI	GGWCC	3 cut(s)	171, 354, 737
SlaI	CTCGAG	1 cut(s)	147
SmiMI	CAYNNNNRTG	2 cut(s)	63, 982
SmlI	CTYRAG	2 cut(s)	147, 579
SmoI	CTYRAG	2 cut(s)	147, 579
Sse9I	AATT	6 cut(s)	175, 372, 452, 671, 684, 806
SseBI	AGGCCT	1 cut(s)	145
SsiI	CCGC	7 cut(s)	131, 198, 200, 203, 215, 481, 862
SspMI	CTAG	4 cut(s)	717, 722, 734, 911
StuI	AGGCCT	1 cut(s)	145
StyD4I	CCNGG	1 cut(s)	946
StyI	CCWWGG	2 cut(s)	540, 721
TaaI	ACNGT	3 cut(s)	529, 559, 775
TaiI	ACGT	1 cut(s)	41
TaqI	TCGA	2 cut(s)	148, 1032
TasI	AATT	6 cut(s)	175, 372, 452, 671, 684, 806
TauI	GCSGC	3 cut(s)	200, 203, 206
TfiI	GAWTC	3 cut(s)	363, 663, 730
Tru1I	TTAA	3 cut(s)	561, 921, 1013
Tru9I	TTAA	3 cut(s)	561, 921, 1013
TseFI	GTSAC	1 cut(s)	775
TseI	GCWGC	4 cut(s)	323, 409, 412, 914
Tsp45I	GTSAC	1 cut(s)	775
TspDTI	ATGAA	2 cut(s)	655, 845
VneI	GTGCAC	1 cut(s)	975
VpaK11BI	GGWCC	3 cut(s)	171, 354, 737
XagI	CCTNNNNNAGG	1 cut(s)	539
XapI	RAATTY	4 cut(s)	372, 452, 671, 806
XcmI	CCANNNNNNNNNTGG	1 cut(s)	364
XhoI	CTCGAG	1 cut(s)	147
XmaJI	CCTAGG	1 cut(s)	721
XmnI	GAANNNNTTC	1 cut(s)	894
XspI	CTAG	4 cut(s)	717, 722, 734, 911

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.