Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0007277

Physical Location & Seq

Genomic Coordinates Forward (+)

3388221 .. 3394048

Gene Structure 5828 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1040200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1713 bp

ATGAAATTCCTCGTACTCGTCTCTGCTTCCGTCGCTCTCGTCGTCGCCGTTCTCGTTCTCAATCCCGGCCACCAGTGGCGGTGGAGTTTGTGGAAGTCTCCGGTGGCGGACATGGTGGTGAGAAATGCCAAGATATACACGAGCGACGAGTCTCTCCCATTCGCCGATTCCATGGCTGTTCGCAACGGAAGGGTTCTTCGTGTTGGCAACTACTCCTCCGTCCAGGATATGGTTGGATATCGGACTAAGGAGCTCGATCTTATGGGAAAGATTGTCGTCCCTGGATTTATTGATTCTCACGTCCATCTCATTTTTGGTGGACTCCAGATGGGGCGTGTGGAACTACGAGGCATAAGTAAAAAAGAGGAATTTCTGAGGCGGGTCAAAGAAGCAGTGAGAAACGCAAAACAAGGTTCTTGGATTTTGGGTGGTGGATGGAACAATGATTTTTGGGGAGGGGAATTGCCAGCGGCTTCTTGGATTGACGATATTACACCTTACAATCCTGTTTGGCTATCAAGGATGGATGGCCATATGGGCTTGGCTAATTCAGTGGCACTTAAGTTGGCTGGCATTACAAATTCATCAGAAGCTCCAATTGGTGGAACTATTATGAAAACAACCAGCGGAGAACCTACTGGACTGCTGATTGATTCAGCAATGAAGCTTCTCCTTCCATCCATTCCAGAGGTATCAGTAGAGGAGCGGAGGGAAGCTATGCTTAGAGCCAGCAATCATGCCTTGATGAGGGGTGTAACAACAGTTGTTGATTTTGGTAGATATTTTCCTGGGGCATCAGCAAAGCAATCCTGGGAAGATTTTTCAGATGTTTATCAATGGGCTGATTGTTCAGGGAAGATGGTGATCAGGGTCTGCTTATTTTTTCCCTTGGAGACTTGGTCACGGTTACATCACCTTGTTAACAAAGTTGGACGTACTCTGAGCCAATGGATTTACTTGGGCGGTGTTAAAGCTTTTGCTGATGGCTCACTGGGTTCAAATAGTGCACTGTTTTATGAGCCATATGCTGATGAGCCTCATAATTATGGCTTGCAAGTGACGGATAATGAAAGTCTCTTCAACCTGACCTTGACTTCGGATAAAGTTGGGCTTCAGGTTGCCATTCATGCCATAGGTGATAAAGCAAATGATTTGATCCTTAACATGTACGAGTCAGTGTTTTCAACAAATGGAGTAAGGGATCGAAGATTCAGGATTGAGCATGCCCAACATCTAGCACCTGGGACACCAGCCCGATTTGGTGAACTCGGGATTGTTGCTTCAGTACAGCCAGAGCACCTATTAGATGACGCTGAATCTGCGACAAAAAAACTTGGGATAGACAGGGCTCACAAGGGATCCTACTTATTCAAGTCACTCTTGGCTGGCAATGGACAGTTGGCACTTGGTTCTGATTTTCCGGTCTCGGATATTAATCCTTTAGGTGGTATTAAGACAGCAATGAAAAGGATTCCCCCTGGTTGGGATACTGCATGGATTCCCACTGAAAGACTTTCTCTGAATGAGGCATTGAAGGGGTACACTCTTTCAGCAGCTCGCGCATGCTTTCTTGACAGTGATTTGGGATCTCTATCGCTCGGAAAACTGGCAGACTTTGTCATACTGTCCACGGATTCGTGGGATGATGCCGTAGCTGAAGAATCTGCATCAGTTGAAGCAACATATGTTGGTGGCGTACAGGCCTATCCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Amidohydro_3

Protein Analysis

571

Amino Acids

62.34

Weight (kDa)

6.04

Isoelectric Point (pI)

32.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Amidohydro_3	PF07969	84 - 569	2e-83	Amidohydrolase family
Amidohydro_1	PF01979	367 - 565	1.4e-08	Amidohydrolase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007277)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55850 AT3G55850 AT3G55850 AT3G55850 AT3G55850 AT3G55850
fragaria_vesca	FvH4_7g03070
malus_domestica	MD07G1040200.v1.1
prunus_persica	Prupe.2G036100_v2.0.a1
pyrus_communis	pycom07g02780
rosa_chinensis	RchiOBHm_Chr1g0324291
rosa_laevigata	RLG00000030207
rosa_multiflora	Rmu_sc0001850.1_g000050 Rmu_sc0031226.1_g000001
rosa_roxburghii	Rroxscaffold_159G00432670 Rroxscaffold_4G00325720
rosa_rugosa	Rorug01G0044600 Rorug01G0044700 Rorug01G0044800
rosa_samantha	Rh1AG060700 Rh1BG051900 Rh1CG063000 Rh1DG067400 Rh4AG012000
rosa_wichuraiana	Rw1G005170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	229, 602
AccBSI	CCGCTC	1 cut(s)	706
AccII	CGCG	1 cut(s)	1560
AciI	CCGC	7 cut(s)	79, 107, 379, 470, 627, 706, 963
AclWI	GGATC	5 cut(s)	1150, 1209, 1353, 1366, 1594
AcoI	YGGCCR	2 cut(s)	67, 529
AcsI	RAATTY	3 cut(s)	5, 368, 580
AcuI	CTGAAG	3 cut(s)	1097, 1266, 1677
AfaI	GTAC	6 cut(s)	15, 937, 1169, 1287, 1541, 1698
AfiI	CCNNNNNNNGG	6 cut(s)	229, 602, 747, 1445, 1482, 1483
AflII	CTTAAG	1 cut(s)	560
AflIII	ACRYGT	1 cut(s)	1164
AgsI	TTSAA	6 cut(s)	999, 1081, 1185, 1372, 1534, 1676
AjiI	CACGTC	1 cut(s)	301
AjnI	CCWGG	6 cut(s)	222, 280, 787, 809, 1240, 1477
AjuI	GAANNNNNNNTTGG	2 cut(s)	582, 614
AluBI	AGCT	7 cut(s)	253, 593, 667, 716, 974, 1556, 1655
AluI	AGCT	7 cut(s)	253, 593, 667, 716, 974, 1556, 1655
Alw21I	GWGCWC	3 cut(s)	255, 1009, 1299
Alw26I	GTCTC	6 cut(s)	25, 102, 156, 887, 1079, 1429
Alw44I	GTGCAC	1 cut(s)	1005
AlwI	GGATC	5 cut(s)	1150, 1209, 1353, 1366, 1594
AlwNI	CAGNNNCTG	1 cut(s)	873
Ama87I	CYCGRG	1 cut(s)	1268
AoxI	GGCC	3 cut(s)	67, 529, 1701
ApaLI	GTGCAC	1 cut(s)	1005
ApeKI	GCWGC	1 cut(s)	1553
ApoI	RAATTY	3 cut(s)	5, 368, 580
ArsI	GACNNNNNNTTYG	2 cut(s)	1078, 1110
AseI	ATTAAT	1 cut(s)	1434
AspLEI	GCGC	1 cut(s)	1562
AsuC2I	CCSGG	1 cut(s)	66
AsuHPI	GGTGA	5 cut(s)	130, 874, 905, 1148, 1274
AvaI	CYCGRG	1 cut(s)	1268
BaeGI	GKGCMC	1 cut(s)	1009
BalI	TGGCCA	1 cut(s)	531
BamHI	GGATCC	1 cut(s)	1358
BanII	GRGCYC	2 cut(s)	255, 1351
BauI	CACGAG	1 cut(s)	139
Bbv12I	GWGCWC	3 cut(s)	255, 1009, 1299
BbvI	GCAGC	1 cut(s)	1565
BccI	CCATC	8 cut(s)	312, 322, 429, 517, 521, 685, 853, 977
BceAI	ACGGC	2 cut(s)	32, 1634
BciT130I	CCWGG	6 cut(s)	224, 282, 789, 811, 1242, 1479
BciVI	GTATCC	1 cut(s)	1480
BclI	TGATCA	1 cut(s)	864
BcnI	CCSGG	1 cut(s)	66
BcoDI	GTCTC	6 cut(s)	25, 102, 156, 887, 1079, 1429
BfaI	CTAG	1 cut(s)	1235
BfrI	CTTAAG	1 cut(s)	560
BfuI	GTATCC	1 cut(s)	1480
BglI	GCCNNNNNGGC	1 cut(s)	537
BisI	GCNGC	2 cut(s)	471, 1554
BlsI	GCNGC	2 cut(s)	472, 1555
Bme1390I	CCNGG	7 cut(s)	66, 224, 282, 789, 811, 1242, 1479
BmeT110I	CYCGRG	1 cut(s)	1268
BmgBI	CACGTC	1 cut(s)	301
BmiI	GGNNCC	1 cut(s)	1360
BmrFI	CCNGG	7 cut(s)	66, 224, 282, 789, 811, 1242, 1479
BmrI	ACTGGG	1 cut(s)	1001
BmsI	GCATC	3 cut(s)	803, 1636, 1676
BmuI	ACTGGG	1 cut(s)	1001
BpmI	CTGGAG	1 cut(s)	308
BpuMI	CCSGG	1 cut(s)	66
BsaBI	GATNNNNATC	4 cut(s)	831, 863, 1434, 1591
BsaI	GGTCTC	1 cut(s)	1429
BsaJI	CCNNGG	8 cut(s)	171, 280, 788, 810, 888, 1241, 1477, 1629
BsaWI	WCCGGW	2 cut(s)	100, 1420
BsaXI	ACNNNNNCTCC	4 cut(s)	200, 230, 884, 914
Bsc4I	CCNNNNNNNGG	6 cut(s)	229, 602, 747, 1445, 1482, 1483
Bse1I	ACTGG	4 cut(s)	73, 643, 996, 1611
Bse3DI	GCAATG	3 cut(s)	666, 1396, 1467
Bse8I	GATNNNNATC	4 cut(s)	831, 863, 1434, 1591
BseBI	CCWGG	6 cut(s)	224, 282, 789, 811, 1242, 1479
BseDI	CCNNGG	8 cut(s)	171, 280, 788, 810, 888, 1241, 1477, 1629
BseGI	GGATG	5 cut(s)	440, 528, 532, 677, 1648
BseJI	GATNNNNATC	4 cut(s)	831, 863, 1434, 1591
BseLI	CCNNNNNNNGG	6 cut(s)	229, 602, 747, 1445, 1482, 1483
BseMI	GCAATG	3 cut(s)	666, 1396, 1467
BseMII	CTCAG	2 cut(s)	365, 932
BseNI	ACTGG	4 cut(s)	73, 643, 996, 1611
BseRI	GAGGAG	2 cut(s)	205, 716
BseSI	GKGCMC	1 cut(s)	1009
BseXI	GCAGC	1 cut(s)	1565
Bsh1236I	CGCG	1 cut(s)	1560
BshFI	GGCC	3 cut(s)	69, 531, 1703
BsiHKAI	GWGCWC	3 cut(s)	255, 1009, 1299
BsiHKCI	CYCGRG	1 cut(s)	1268
BsiSI	CCGG	3 cut(s)	66, 101, 1421
BslFI	GGGAC	2 cut(s)	263, 1258
BslI	CCNNNNNNNGG	6 cut(s)	229, 602, 747, 1445, 1482, 1483
BsmAI	GTCTC	6 cut(s)	25, 102, 156, 887, 1079, 1429
BsmBI	CGTCTC	1 cut(s)	25
BsmFI	GGGAC	2 cut(s)	263, 1258
BsnI	GGCC	3 cut(s)	69, 531, 1703
Bso31I	GGTCTC	1 cut(s)	1429
BsoBI	CYCGRG	1 cut(s)	1268
Bsp1286I	GDGCHC	4 cut(s)	255, 1009, 1299, 1351
Bsp143I	GATC	6 cut(s)	256, 864, 1155, 1201, 1358, 1586
Bsp19I	CCATGG	1 cut(s)	171
BspACI	CCGC	7 cut(s)	79, 107, 379, 470, 627, 706, 963
BspANI	GGCC	3 cut(s)	69, 531, 1703
BspCNI	CTCAG	2 cut(s)	366, 933
BspFNI	CGCG	1 cut(s)	1560
BspLI	GGNNCC	1 cut(s)	1360
BspPI	GGATC	5 cut(s)	1150, 1209, 1353, 1366, 1594
BspTI	CTTAAG	1 cut(s)	560
BspTNI	GGTCTC	1 cut(s)	1429
BsrBI	CCGCTC	1 cut(s)	706
BsrDI	GCAATG	3 cut(s)	666, 1396, 1467
BsrI	ACTGG	4 cut(s)	73, 643, 996, 1611
BssECI	CCNNGG	8 cut(s)	171, 280, 788, 810, 888, 1241, 1477, 1629
BssMI	GATC	6 cut(s)	256, 864, 1155, 1201, 1358, 1586
BssSI	CACGAG	1 cut(s)	139
BssT1I	CCWWGG	2 cut(s)	171, 888
Bst2BI	CACGAG	1 cut(s)	139
Bst2UI	CCWGG	6 cut(s)	224, 282, 789, 811, 1242, 1479
Bst4CI	ACNGT	6 cut(s)	763, 906, 1011, 1398, 1577, 1626
Bst6I	CTCTTC	1 cut(s)	1082
BstAFI	CTTAAG	1 cut(s)	560
BstC8I	GCNNGC	8 cut(s)	468, 571, 730, 1052, 1224, 1387, 1558, 1564
BstDEI	CTNAG	4 cut(s)	246, 374, 722, 941
BstDSI	CCRYGG	2 cut(s)	171, 1629
BstENI	CCTNNNNNAGG	1 cut(s)	745
BstF5I	GGATG	5 cut(s)	440, 528, 532, 677, 1648
BstFNI	CGCG	1 cut(s)	1560
BstHHI	GCGC	1 cut(s)	1562
BstKTI	GATC	6 cut(s)	259, 867, 1158, 1204, 1361, 1589
BstMAI	GTCTC	6 cut(s)	25, 102, 156, 887, 1079, 1429
BstMBI	GATC	6 cut(s)	256, 864, 1155, 1201, 1358, 1586
BstMWI	GCNNNNNNNGC	5 cut(s)	32, 537, 1127, 1319, 1559
BstNI	CCWGG	6 cut(s)	224, 282, 789, 811, 1242, 1479
BstNSI	RCATGY	3 cut(s)	1168, 1226, 1566
BstSCI	CCNGG	7 cut(s)	64, 222, 280, 787, 809, 1240, 1477
BstSLI	GKGCMC	1 cut(s)	1009
BstUI	CGCG	1 cut(s)	1560
BstV1I	GCAGC	1 cut(s)	1565
BstX2I	RGATCY	2 cut(s)	1358, 1586
BstYI	RGATCY	2 cut(s)	1358, 1586
BsuI	GTATCC	1 cut(s)	1480
BsuRI	GGCC	3 cut(s)	69, 531, 1703
BtgI	CCRYGG	2 cut(s)	171, 1629
BtrI	CACGTC	1 cut(s)	301
BtsCI	GGATG	5 cut(s)	440, 528, 532, 677, 1648
BtsI	GCAGTG	1 cut(s)	399
BtsIMutI	CAGTG	8 cut(s)	80, 399, 558, 989, 1007, 1182, 1503, 1582
Cac8I	GCNNGC	8 cut(s)	468, 571, 730, 1052, 1224, 1387, 1558, 1564
CaiI	CAGNNNCTG	1 cut(s)	873
CfoI	GCGC	1 cut(s)	1562
CseI	GACGC	1 cut(s)	1319
Csp6I	GTAC	6 cut(s)	14, 936, 1168, 1286, 1540, 1697
CviAII	CATG	8 cut(s)	112, 172, 737, 1127, 1165, 1223, 1494, 1563
CviQI	GTAC	6 cut(s)	14, 936, 1168, 1286, 1540, 1697
DdeI	CTNAG	4 cut(s)	246, 374, 722, 941
DpnI	GATC	6 cut(s)	258, 866, 1157, 1203, 1360, 1588
DpnII	GATC	6 cut(s)	256, 864, 1155, 1201, 1358, 1586
EaeI	YGGCCR	2 cut(s)	67, 529
Eam1104I	CTCTTC	1 cut(s)	1082
EarI	CTCTTC	1 cut(s)	1082
EciI	GGCGGA	1 cut(s)	122
Ecl136II	GAGCTC	1 cut(s)	253
Eco130I	CCWWGG	2 cut(s)	171, 888
Eco147I	AGGCCT	1 cut(s)	1703
Eco24I	GRGCYC	2 cut(s)	255, 1351
Eco31I	GGTCTC	1 cut(s)	1429
Eco32I	GATATC	1 cut(s)	239
Eco53kI	GAGCTC	1 cut(s)	253
Eco57I	CTGAAG	3 cut(s)	1097, 1266, 1677
Eco88I	CYCGRG	1 cut(s)	1268
EcoICRI	GAGCTC	1 cut(s)	253
EcoNI	CCTNNNNNAGG	1 cut(s)	745
EcoRII	CCWGG	6 cut(s)	222, 280, 787, 809, 1240, 1477
EcoRV	GATATC	1 cut(s)	239
EcoT14I	CCWWGG	2 cut(s)	171, 888
EcoT38I	GRGCYC	2 cut(s)	255, 1351
ErhI	CCWWGG	2 cut(s)	171, 888
Esp3I	CGTCTC	1 cut(s)	25
FaeI	CATG	8 cut(s)	115, 175, 740, 1130, 1168, 1226, 1497, 1566
FalI	AAGNNNNNCTT	6 cut(s)	705, 737, 1095, 1127, 1364, 1396
FaqI	GGGAC	2 cut(s)	263, 1258
FatI	CATG	8 cut(s)	111, 171, 736, 1126, 1164, 1222, 1493, 1562
FauI	CCCGC	1 cut(s)	372
FauNDI	CATATG	3 cut(s)	534, 1024, 1684
FbaI	TGATCA	1 cut(s)	864
Fnu4HI	GCNGC	2 cut(s)	471, 1554
FokI	GGATG	5 cut(s)	447, 535, 539, 664, 1655
FriOI	GRGCYC	2 cut(s)	255, 1351
Fsp4HI	GCNGC	2 cut(s)	471, 1554
FspBI	CTAG	1 cut(s)	1235
GlaI	GCGC	1 cut(s)	1561
GluI	GCNGC	2 cut(s)	471, 1554
GsuI	CTGGAG	1 cut(s)	308
HaeIII	GGCC	3 cut(s)	69, 531, 1703
HapII	CCGG	3 cut(s)	66, 101, 1421
HgaI	GACGC	1 cut(s)	1319
HhaI	GCGC	1 cut(s)	1562
Hin1II	CATG	8 cut(s)	115, 175, 740, 1130, 1168, 1226, 1497, 1566
Hin6I	GCGC	1 cut(s)	1560
HinP1I	GCGC	1 cut(s)	1560
HincII	GTYRAC	1 cut(s)	922
HindII	GTYRAC	1 cut(s)	922
HindIII	AAGCTT	2 cut(s)	665, 972
HpaI	GTTAAC	1 cut(s)	922
HpaII	CCGG	3 cut(s)	66, 101, 1421
HphI	GGTGA	5 cut(s)	130, 874, 905, 1148, 1274
Hpy166II	GTNNAC	6 cut(s)	320, 922, 1007, 1265, 1542, 1629
Hpy188III	TCNNGA	5 cut(s)	325, 686, 1213, 1270, 1571
Hpy8I	GTNNAC	6 cut(s)	320, 922, 1007, 1265, 1542, 1629
Hpy99I	CGWCG	4 cut(s)	35, 44, 47, 149
HpyAV	CCTTC	3 cut(s)	183, 683, 1528
HpyCH4III	ACNGT	6 cut(s)	763, 906, 1011, 1398, 1577, 1626
HpyCH4IV	ACGT	2 cut(s)	300, 934
HpyCH4V	TGCA	4 cut(s)	1007, 1054, 1493, 1667
HpyF10VI	GCNNNNNNNGC	5 cut(s)	32, 537, 1127, 1319, 1559
HpyF3I	CTNAG	4 cut(s)	246, 374, 722, 941
HpySE526I	ACGT	2 cut(s)	300, 934
Hsp92II	CATG	8 cut(s)	115, 175, 740, 1130, 1168, 1226, 1497, 1566
HspAI	GCGC	1 cut(s)	1560
Ksp22I	TGATCA	1 cut(s)	864
KspAI	GTTAAC	1 cut(s)	922
Kzo9I	GATC	6 cut(s)	256, 864, 1155, 1201, 1358, 1586
LmnI	GCTCC	3 cut(s)	250, 598, 703
Lsp1109I	GCAGC	1 cut(s)	1565
LweI	GCATC	3 cut(s)	803, 1636, 1676
MaeI	CTAG	1 cut(s)	1235
MaeII	ACGT	2 cut(s)	300, 934
MaeIII	GTNAC	5 cut(s)	754, 900, 906, 1057, 1374
MalI	GATC	6 cut(s)	258, 866, 1157, 1203, 1360, 1588
MbiI	CCGCTC	1 cut(s)	706
MboI	GATC	6 cut(s)	256, 864, 1155, 1201, 1358, 1586
MboII	GAAGA	6 cut(s)	188, 827, 868, 1069, 1218, 1670
MfeI	CAATTG	1 cut(s)	597
MflI	RGATCY	2 cut(s)	1358, 1586
MhlI	GDGCHC	4 cut(s)	255, 1009, 1299, 1351
MlsI	TGGCCA	1 cut(s)	531
MluCI	AATT	7 cut(s)	5, 368, 461, 547, 580, 597, 1042
MluNI	TGGCCA	1 cut(s)	531
MlyI	GAGTC	3 cut(s)	158, 315, 1181
MmeI	TCCRAC	2 cut(s)	214, 910
Mox20I	TGGCCA	1 cut(s)	531
MscI	TGGCCA	1 cut(s)	531
MseI	TTAA	7 cut(s)	561, 921, 969, 1161, 1434, 1452, 1711
MslI	CAYNNNNRTG	2 cut(s)	116, 1044
Msp20I	TGGCCA	1 cut(s)	531
MspA1I	CMGCKG	2 cut(s)	470, 627
MspCI	CTTAAG	1 cut(s)	560
MspI	CCGG	3 cut(s)	66, 101, 1421
MspR9I	CCNGG	7 cut(s)	66, 224, 282, 789, 811, 1242, 1479
MunI	CAATTG	1 cut(s)	597
MvaI	CCWGG	6 cut(s)	224, 282, 789, 811, 1242, 1479
MvnI	CGCG	1 cut(s)	1560
MwoI	GCNNNNNNNGC	5 cut(s)	32, 537, 1127, 1319, 1559
NciI	CCSGG	1 cut(s)	66
NcoI	CCATGG	1 cut(s)	171
NdeI	CATATG	3 cut(s)	534, 1024, 1684
NdeII	GATC	6 cut(s)	256, 864, 1155, 1201, 1358, 1586
NlaIII	CATG	8 cut(s)	115, 175, 740, 1130, 1168, 1226, 1497, 1566
NlaIV	GGNNCC	1 cut(s)	1360
NmuCI	GTSAC	3 cut(s)	900, 1057, 1374
NspI	RCATGY	3 cut(s)	1168, 1226, 1566
PaeI	GCATGC	2 cut(s)	1226, 1566
PceI	AGGCCT	1 cut(s)	1703
PciI	ACATGT	1 cut(s)	1164
PcsI	WCGNNNNNNNCGW	3 cut(s)	39, 45, 51
PfeI	GAWTC	9 cut(s)	167, 293, 653, 1209, 1316, 1471, 1498, 1634, 1661
PflFI	GACNNNGTC	2 cut(s)	898, 1616
PflMI	CCANNNNNTGG	2 cut(s)	229, 602
PfoI	TCCNGGA	1 cut(s)	222
PkrI	GCNGC	2 cut(s)	472, 1555
PleI	GAGTC	3 cut(s)	157, 315, 1180
PpsI	GAGTC	3 cut(s)	157, 315, 1180
PscI	ACATGT	1 cut(s)	1164
PshBI	ATTAAT	1 cut(s)	1434
Psp124BI	GAGCTC	1 cut(s)	255
Psp6I	CCWGG	6 cut(s)	222, 280, 787, 809, 1240, 1477
PspGI	CCWGG	6 cut(s)	222, 280, 787, 809, 1240, 1477
PspN4I	GGNNCC	1 cut(s)	1360
PstNI	CAGNNNCTG	1 cut(s)	873
PsuI	RGATCY	2 cut(s)	1358, 1586
PsyI	GACNNNGTC	2 cut(s)	898, 1616
RsaI	GTAC	6 cut(s)	15, 937, 1169, 1287, 1541, 1698
RsaNI	GTAC	6 cut(s)	14, 936, 1168, 1286, 1540, 1697
RseI	CAYNNNNRTG	2 cut(s)	116, 1044
SacI	GAGCTC	1 cut(s)	255
SaqAI	TTAA	7 cut(s)	561, 921, 969, 1161, 1434, 1452, 1711
SatI	GCNGC	2 cut(s)	471, 1554
Sau3AI	GATC	6 cut(s)	256, 864, 1155, 1201, 1358, 1586
SchI	GAGTC	3 cut(s)	158, 315, 1181
ScrFI	CCNGG	7 cut(s)	66, 224, 282, 789, 811, 1242, 1479
SduI	GDGCHC	4 cut(s)	255, 1009, 1299, 1351
SfaNI	GCATC	3 cut(s)	803, 1636, 1676
SmiMI	CAYNNNNRTG	2 cut(s)	116, 1044
SmlI	CTYRAG	1 cut(s)	560
SmoI	CTYRAG	1 cut(s)	560
SphI	GCATGC	2 cut(s)	1226, 1566
Sse9I	AATT	7 cut(s)	5, 368, 461, 547, 580, 597, 1042
SseBI	AGGCCT	1 cut(s)	1703
SsiI	CCGC	7 cut(s)	79, 107, 379, 470, 627, 706, 963
SspMI	CTAG	1 cut(s)	1235
SstI	GAGCTC	1 cut(s)	255
StuI	AGGCCT	1 cut(s)	1703
StyD4I	CCNGG	7 cut(s)	64, 222, 280, 787, 809, 1240, 1477
StyI	CCWWGG	2 cut(s)	171, 888
TaaI	ACNGT	6 cut(s)	763, 906, 1011, 1398, 1577, 1626
TaiI	ACGT	2 cut(s)	303, 937
TaqI	TCGA	2 cut(s)	255, 1204
TasI	AATT	7 cut(s)	5, 368, 461, 547, 580, 597, 1042
TatI	WGTACW	1 cut(s)	1285
TauI	GCSGC	1 cut(s)	473
TfiI	GAWTC	9 cut(s)	167, 293, 653, 1209, 1316, 1471, 1498, 1634, 1661
Tru1I	TTAA	7 cut(s)	561, 921, 969, 1161, 1434, 1452, 1711
Tru9I	TTAA	7 cut(s)	561, 921, 969, 1161, 1434, 1452, 1711
TscAI	CASTG	8 cut(s)	80, 399, 558, 996, 1014, 1182, 1510, 1582
TseFI	GTSAC	3 cut(s)	900, 1057, 1374
TseI	GCWGC	1 cut(s)	1553
Tsp45I	GTSAC	3 cut(s)	900, 1057, 1374
TspDTI	ATGAA	7 cut(s)	17, 573, 629, 677, 1083, 1115, 1478
TspGWI	ACGGA	5 cut(s)	19, 201, 208, 1076, 1646
TspRI	CASTG	8 cut(s)	80, 399, 558, 996, 1014, 1182, 1510, 1582
Tth111I	GACNNNGTC	2 cut(s)	898, 1616
Van91I	CCANNNNNTGG	2 cut(s)	229, 602
Vha464I	CTTAAG	1 cut(s)	560
VneI	GTGCAC	1 cut(s)	1005
VspI	ATTAAT	1 cut(s)	1434
XagI	CCTNNNNNAGG	1 cut(s)	745
XapI	RAATTY	3 cut(s)	5, 368, 580
XceI	RCATGY	3 cut(s)	1168, 1226, 1566
XcmI	CCANNNNNNNNNTGG	3 cut(s)	230, 311, 474
XspI	CTAG	1 cut(s)	1235

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.