Rh1CG063000

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0007277
Physical Location & Seq
Forward (+)
12474123 .. 12484003
9881 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG063000.1

Sequence Viewer

Length: 1338 bp
ATGAAGTCTGCTCTCCTACTCCTCTCAGCTTTTGCTGCTCTCCTCCTCGCCATTTTCGCTTCTCTCAATCCCAACCGCCAGTGGTTCAGTTGGAGGTCACCGGCGGCGGACTTGGTGGTGAAGAATGCCAAGATATACACCGTCGACGAGTCTCTCCCCTTCGCCGATTCCATGGCCGTTCGCAACGGAAGGATTCTCCGGATTGGAAGCTACTCCTCAGTTCAGGATATGGTAGGGTATGGGACTAAAGAGTTAGATCTTAAGGGAAAAATTGTAGTCCCTGGATTTATTGATTCCCATGTGCATCTGATCTTTGGTGGACTGCAGATGATGCGTGTAGAACTACATGGCATAAACTCAAAAGATGAATTTGTGAAGAGGATCAAAGAAGCTGTGAGAAATGCAAAACAAGGTTCCTGGATTTTGGGCGGTGGATGGAACAATGATCTATGGGGAGGTGAGTTGCCATTGGCTTCTTGGATTGACGATATTACACCTTATAATCCTGTCTTTCTATCAAGAATGGATGGGCATATGAGCTTGGCTAATTCAGTGGCACTCAAGTTGGGTGGGATTACTAAATCATCAGTGGATCCAAATGGTGGAACCATCATGAAAACCACCAGTGGAGAACCTACTGGACTGTTGATTGATTCTGCAATGGAACTTATCCTTCCACTCATTCCAGAGGTCTCAGTAGAGGAGCGGAGGGAAGCTTTGCGTAAAGCCAGCAATCATGCCTTGATGAGGGGTGTGACGACAGTTGTTGATGTTGGTAGATACTTTCCTGGGACTTCTGTAAAAGATTCCTGGGAAGATTTTTCAGATGTGTATCAATGGGCGGATTTGTCAGGGAAGATGACGATCAGGGTCTGCTTATTTTTCCCACTGGAGACTTGGTCACGTTTACGTGATCTTATTAACAATGTTGGCCAATCTATGAGCCAGTGGATTTATTTGGGTGGGGTTAAAGCATTTGCTGATGGGTCATTGGGTTCTCGTAGTGCACTTTTCTATGAGCCGTATGTTGATGAGCCTCATAATTATGGCTTACAAGTAGGAGATTATGAAAGTCTCCACAACCTGACCTTAGCATCAGATAGAGTTGGGCTTCAGGTTGCTATTCATGCTATAGGCGATCGAGCAAATGACTTGATCCTCGACATGTATGAGTCAGTCATTTCAAAAAACGGAGTGAGGGATCGAAGATTCAGGATTGAGCATGCGCAACATCTGGCACCTGGGACACCAGCCAGATTCGGTAAACTAGGGATTGTTGCTTCAGTACAGGTTGATCACACTACTCCTTGGTATCTTATCATTTTTGTAATTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidohydro_3

Protein Analysis

445

Amino Acids

49.27

Weight (kDa)

6.43

Isoelectric Point (pI)

33.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_3 PF07969 84 - 435 8.6e-62 Amidohydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 501
Acc16I TGCGCA 1 cut(s) 1227
AccB1I GGYRCC 1 cut(s) 1237
AccB7I CCANNNNNTGG 1 cut(s) 602
AccBSI CCGCTC 1 cut(s) 706
AccI GTMKAC 1 cut(s) 144
AccIII TCCGGA 1 cut(s) 198
AciI CCGC 6 cut(s) 76, 104, 107, 429, 706, 842
AclWI GGATC 5 cut(s) 389, 587, 600, 1150, 1209
AcoI YGGCCR 2 cut(s) 174, 931
AcsI RAATTY 1 cut(s) 368
AcuI CTGAAG 2 cut(s) 1097, 1266
AfaI GTAC 1 cut(s) 1287
AfiI CCNNNNNNNGG 2 cut(s) 602, 747
AflII CTTAAG 1 cut(s) 260
AflIII ACRYGT 1 cut(s) 1164
AgsI TTSAA 1 cut(s) 1185
AjnI CCWGG 5 cut(s) 280, 416, 787, 809, 1240
AluBI AGCT 5 cut(s) 29, 210, 392, 540, 716
AluI AGCT 5 cut(s) 29, 210, 392, 540, 716
Alw21I GWGCWC 1 cut(s) 1009
Alw26I GTCTC 4 cut(s) 156, 697, 887, 1079
Alw44I GTGCAC 1 cut(s) 1005
AlwI GGATC 5 cut(s) 389, 587, 600, 1150, 1209
AlwNI CAGNNNCTG 1 cut(s) 873
Aor13HI TCCGGA 1 cut(s) 198
AoxI GGCC 2 cut(s) 174, 931
ApaLI GTGCAC 1 cut(s) 1005
ApeKI GCWGC 1 cut(s) 35
ApoI RAATTY 1 cut(s) 368
AseI ATTAAT 1 cut(s) 1332
AspLEI GCGC 1 cut(s) 1228
AsuHPI GGTGA 3 cut(s) 90, 130, 470
BaeGI GKGCMC 1 cut(s) 1009
BalI TGGCCA 1 cut(s) 933
BamHI GGATCC 1 cut(s) 592
BanI GGYRCC 1 cut(s) 1237
Bbv12I GWGCWC 1 cut(s) 1009
BbvI GCAGC 1 cut(s) 22
BccI CCATC 4 cut(s) 429, 521, 617, 977
BceAI ACGGC 2 cut(s) 161, 1006
BciT130I CCWGG 5 cut(s) 282, 418, 789, 811, 1242
BclI TGATCA 1 cut(s) 1294
BcoDI GTCTC 4 cut(s) 156, 697, 887, 1079
BfaI CTAG 1 cut(s) 1268
BfmI CTRYAG 2 cut(s) 323, 1131
BfrI CTTAAG 1 cut(s) 260
BglII AGATCT 1 cut(s) 256
BisI GCNGC 2 cut(s) 36, 105
BlsI GCNGC 2 cut(s) 37, 106
Bme1390I CCNGG 5 cut(s) 282, 418, 789, 811, 1242
BmiI GGNNCC 4 cut(s) 415, 594, 607, 1239
BmrFI CCNGG 5 cut(s) 282, 418, 789, 811, 1242
BmsI GCATC 3 cut(s) 313, 321, 1103
BpmI CTGGAG 1 cut(s) 911
Bpu10I CCTNAGC 1 cut(s) 1090
BpuEI CTTGAG 1 cut(s) 545
BsaAI YACGTR 1 cut(s) 911
BsaBI GATNNNNATC 2 cut(s) 831, 863
BsaI GGTCTC 1 cut(s) 697
BsaJI CCNNGG 6 cut(s) 171, 280, 788, 810, 1241, 1307
BsaWI WCCGGW 1 cut(s) 198
BsaXI ACNNNNNCTCC 2 cut(s) 884, 914
Bsc4I CCNNNNNNNGG 2 cut(s) 602, 747
Bse118I RCCGGY 1 cut(s) 100
Bse1I ACTGG 5 cut(s) 79, 624, 643, 894, 946
Bse3DI GCAATG 1 cut(s) 666
Bse8I GATNNNNATC 2 cut(s) 831, 863
BseAI TCCGGA 1 cut(s) 198
BseBI CCWGG 5 cut(s) 282, 418, 789, 811, 1242
BseDI CCNNGG 6 cut(s) 171, 280, 788, 810, 1241, 1307
BseGI GGATG 2 cut(s) 440, 532
BseJI GATNNNNATC 2 cut(s) 831, 863
BseLI CCNNNNNNNGG 2 cut(s) 602, 747
BseMI GCAATG 1 cut(s) 666
BseMII CTCAG 3 cut(s) 39, 231, 708
BseNI ACTGG 5 cut(s) 79, 624, 643, 894, 946
BseRI GAGGAG 5 cut(s) 11, 32, 35, 205, 716
BseSI GKGCMC 1 cut(s) 1009
BseXI GCAGC 1 cut(s) 22
Bsh1285I CGRYCG 1 cut(s) 1141
BshFI GGCC 2 cut(s) 176, 933
BshNI GGYRCC 1 cut(s) 1237
BsiEI CGRYCG 1 cut(s) 1141
BsiHKAI GWGCWC 1 cut(s) 1009
BsiSI CCGG 2 cut(s) 101, 199
BslFI GGGAC 4 cut(s) 256, 263, 805, 1258
BslI CCNNNNNNNGG 2 cut(s) 602, 747
BsmAI GTCTC 4 cut(s) 156, 697, 887, 1079
BsmFI GGGAC 4 cut(s) 256, 263, 805, 1258
BsmI GAATGC 1 cut(s) 130
BsnI GGCC 2 cut(s) 176, 933
Bso31I GGTCTC 1 cut(s) 697
Bsp1286I GDGCHC 1 cut(s) 1009
Bsp13I TCCGGA 1 cut(s) 198
Bsp19I CCATGG 1 cut(s) 171
BspACI CCGC 6 cut(s) 76, 104, 107, 429, 706, 842
BspANI GGCC 2 cut(s) 176, 933
BspCNI CTCAG 3 cut(s) 38, 230, 707
BspEI TCCGGA 1 cut(s) 198
BspHI TCATGA 1 cut(s) 612
BspLI GGNNCC 4 cut(s) 415, 594, 607, 1239
BspMAI CTGCAG 1 cut(s) 327
BspPI GGATC 5 cut(s) 389, 587, 600, 1150, 1209
BspT107I GGYRCC 1 cut(s) 1237
BspTI CTTAAG 1 cut(s) 260
BspTNI GGTCTC 1 cut(s) 697
BsrBI CCGCTC 1 cut(s) 706
BsrDI GCAATG 1 cut(s) 666
BsrFI RCCGGY 1 cut(s) 100
BsrI ACTGG 5 cut(s) 79, 624, 643, 894, 946
BssAI RCCGGY 1 cut(s) 100
BssECI CCNNGG 6 cut(s) 171, 280, 788, 810, 1241, 1307
BssT1I CCWWGG 2 cut(s) 171, 1307
Bst2UI CCWGG 5 cut(s) 282, 418, 789, 811, 1242
Bst4CI ACNGT 3 cut(s) 142, 645, 763
Bst6I CTCTTC 1 cut(s) 371
BstAFI CTTAAG 1 cut(s) 260
BstAPI GCANNNNNTGC 1 cut(s) 331
BstBAI YACGTR 1 cut(s) 911
BstC8I GCNNGC 2 cut(s) 730, 1224
BstDEI CTNAG 4 cut(s) 25, 217, 694, 1090
BstDSI CCRYGG 1 cut(s) 171
BstEII GGTNACC 1 cut(s) 96
BstENI CCTNNNNNAGG 1 cut(s) 745
BstF5I GGATG 2 cut(s) 440, 532
BstHHI GCGC 1 cut(s) 1228
BstMAI GTCTC 4 cut(s) 156, 697, 887, 1079
BstMCI CGRYCG 1 cut(s) 1141
BstMWI GCNNNNNNNGC 4 cut(s) 35, 56, 331, 1127
BstNI CCWGG 5 cut(s) 282, 418, 789, 811, 1242
BstNSI RCATGY 2 cut(s) 1168, 1226
BstPI GGTNACC 1 cut(s) 96
BstSCI CCNGG 5 cut(s) 280, 416, 787, 809, 1240
BstSFI CTRYAG 2 cut(s) 323, 1131
BstSLI GKGCMC 1 cut(s) 1009
BstV1I GCAGC 1 cut(s) 22
BstX2I RGATCY 2 cut(s) 256, 592
BstYI RGATCY 2 cut(s) 256, 592
BsuRI GGCC 2 cut(s) 176, 933
BtgI CCRYGG 1 cut(s) 171
BtsCI GGATG 2 cut(s) 440, 532
BtsIMutI CAGTG 6 cut(s) 86, 558, 594, 631, 887, 953
Cac8I GCNNGC 2 cut(s) 730, 1224
CaiI CAGNNNCTG 1 cut(s) 873
CciI TCATGA 1 cut(s) 612
CfoI GCGC 1 cut(s) 1228
Cfr10I RCCGGY 1 cut(s) 100
Csp6I GTAC 1 cut(s) 1286
CspCI CAANNNNNGTGG 2 cut(s) 550, 585
CviAII CATG 8 cut(s) 172, 299, 347, 613, 737, 1127, 1165, 1223
CviQI GTAC 1 cut(s) 1286
DdeI CTNAG 4 cut(s) 25, 217, 694, 1090
EaeI YGGCCR 2 cut(s) 174, 931
Eam1104I CTCTTC 1 cut(s) 371
EarI CTCTTC 1 cut(s) 371
EciI GGCGGA 2 cut(s) 122, 857
Eco130I CCWWGG 2 cut(s) 171, 1307
Eco31I GGTCTC 1 cut(s) 697
Eco57I CTGAAG 2 cut(s) 1097, 1266
Eco91I GGTNACC 1 cut(s) 96
EcoNI CCTNNNNNAGG 1 cut(s) 745
EcoO65I GGTNACC 1 cut(s) 96
EcoRII CCWGG 5 cut(s) 280, 416, 787, 809, 1240
EcoT14I CCWWGG 2 cut(s) 171, 1307
ErhI CCWWGG 2 cut(s) 171, 1307
FaeI CATG 8 cut(s) 175, 302, 350, 616, 740, 1130, 1168, 1226
FaqI GGGAC 4 cut(s) 256, 263, 805, 1258
FatI CATG 8 cut(s) 171, 298, 346, 612, 736, 1126, 1164, 1222
FauNDI CATATG 1 cut(s) 534
FbaI TGATCA 1 cut(s) 1294
FblI GTMKAC 1 cut(s) 144
Fnu4HI GCNGC 2 cut(s) 36, 105
FokI GGATG 2 cut(s) 447, 539
Fsp4HI GCNGC 2 cut(s) 36, 105
FspBI CTAG 1 cut(s) 1268
FspI TGCGCA 1 cut(s) 1227
GlaI GCGC 1 cut(s) 1227
GluI GCNGC 2 cut(s) 36, 105
GsuI CTGGAG 1 cut(s) 911
HaeIII GGCC 2 cut(s) 176, 933
HapII CCGG 2 cut(s) 101, 199
HhaI GCGC 1 cut(s) 1228
Hin1II CATG 8 cut(s) 175, 302, 350, 616, 740, 1130, 1168, 1226
Hin6I GCGC 1 cut(s) 1226
HinP1I GCGC 1 cut(s) 1226
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HindIII AAGCTT 1 cut(s) 714
HinfI GANTC 9 cut(s) 149, 167, 193, 293, 653, 806, 1172, 1209, 1257
HpaII CCGG 2 cut(s) 101, 199
HphI GGTGA 3 cut(s) 90, 130, 470
Hpy166II GTNNAC 5 cut(s) 145, 320, 908, 1007, 1265
Hpy188I TCNGA 3 cut(s) 309, 826, 1099
Hpy188III TCNNGA 6 cut(s) 199, 224, 519, 613, 686, 1213
Hpy8I GTNNAC 5 cut(s) 145, 320, 908, 1007, 1265
Hpy99I CGWCG 2 cut(s) 146, 149
HpyAV CCTTC 3 cut(s) 169, 183, 683
HpyCH4III ACNGT 3 cut(s) 142, 645, 763
HpyCH4IV ACGT 2 cut(s) 904, 910
HpyCH4V TGCA 5 cut(s) 304, 325, 404, 659, 1007
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 56, 331, 1127
HpyF3I CTNAG 4 cut(s) 25, 217, 694, 1090
HpySE526I ACGT 2 cut(s) 904, 910
Hsp92II CATG 8 cut(s) 175, 302, 350, 616, 740, 1130, 1168, 1226
HspAI GCGC 1 cut(s) 1226
Kpn2I TCCGGA 1 cut(s) 198
Ksp22I TGATCA 1 cut(s) 1294
LmnI GCTCC 1 cut(s) 703
Lsp1109I GCAGC 1 cut(s) 22
LweI GCATC 3 cut(s) 313, 321, 1103
MaeI CTAG 1 cut(s) 1268
MaeII ACGT 2 cut(s) 904, 910
MaeIII GTNAC 3 cut(s) 96, 754, 900
MbiI CCGCTC 1 cut(s) 706
MboII GAAGA 5 cut(s) 133, 388, 827, 868, 1218
MflI RGATCY 2 cut(s) 256, 592
MhlI GDGCHC 1 cut(s) 1009
MlsI TGGCCA 1 cut(s) 933
MluCI AATT 6 cut(s) 270, 368, 547, 1042, 1329, 1333
MluNI TGGCCA 1 cut(s) 933
MlyI GAGTC 2 cut(s) 158, 1181
MmeI TCCRAC 1 cut(s) 71
Mox20I TGGCCA 1 cut(s) 933
MroI TCCGGA 1 cut(s) 198
MscI TGGCCA 1 cut(s) 933
MseI TTAA 4 cut(s) 261, 921, 969, 1332
MslI CAYNNNNRTG 1 cut(s) 1044
Msp20I TGGCCA 1 cut(s) 933
MspCI CTTAAG 1 cut(s) 260
MspI CCGG 2 cut(s) 101, 199
MspR9I CCNGG 5 cut(s) 282, 418, 789, 811, 1242
Mva1269I GAATGC 1 cut(s) 130
MvaI CCWGG 5 cut(s) 282, 418, 789, 811, 1242
MwoI GCNNNNNNNGC 4 cut(s) 35, 56, 331, 1127
NcoI CCATGG 1 cut(s) 171
NdeI CATATG 1 cut(s) 534
NlaIII CATG 8 cut(s) 175, 302, 350, 616, 740, 1130, 1168, 1226
NlaIV GGNNCC 4 cut(s) 415, 594, 607, 1239
NmuCI GTSAC 3 cut(s) 96, 754, 900
NsbI TGCGCA 1 cut(s) 1227
NspI RCATGY 2 cut(s) 1168, 1226
PaeI GCATGC 1 cut(s) 1226
PagI TCATGA 1 cut(s) 612
PciI ACATGT 1 cut(s) 1164
PctI GAATGC 1 cut(s) 130
PfeI GAWTC 7 cut(s) 167, 193, 293, 653, 806, 1209, 1257
PflFI GACNNNGTC 1 cut(s) 898
PflMI CCANNNNNTGG 1 cut(s) 602
PfoI TCCNGGA 1 cut(s) 416
PkrI GCNGC 2 cut(s) 37, 106
Ple19I CGATCG 1 cut(s) 1141
PleI GAGTC 2 cut(s) 157, 1180
PpsI GAGTC 2 cut(s) 157, 1180
Ppu21I YACGTR 1 cut(s) 911
PscI ACATGT 1 cut(s) 1164
PshBI ATTAAT 1 cut(s) 1332
PsiI TTATAA 1 cut(s) 501
Psp6I CCWGG 5 cut(s) 280, 416, 787, 809, 1240
PspEI GGTNACC 1 cut(s) 96
PspGI CCWGG 5 cut(s) 280, 416, 787, 809, 1240
PspN4I GGNNCC 4 cut(s) 415, 594, 607, 1239
PstI CTGCAG 1 cut(s) 327
PstNI CAGNNNCTG 1 cut(s) 873
PsuI RGATCY 2 cut(s) 256, 592
PsyI GACNNNGTC 1 cut(s) 898
PvuI CGATCG 1 cut(s) 1141
RsaI GTAC 1 cut(s) 1287
RsaNI GTAC 1 cut(s) 1286
RseI CAYNNNNRTG 1 cut(s) 1044
SalI GTCGAC 1 cut(s) 143
SaqAI TTAA 4 cut(s) 261, 921, 969, 1332
SatI GCNGC 2 cut(s) 36, 105
SchI GAGTC 2 cut(s) 158, 1181
ScrFI CCNGG 5 cut(s) 282, 418, 789, 811, 1242
SduI GDGCHC 1 cut(s) 1009
SfaNI GCATC 3 cut(s) 313, 321, 1103
SfcI CTRYAG 2 cut(s) 323, 1131
SgrAI CRCCGGYG 1 cut(s) 100
SgrDI CGTCGACG 1 cut(s) 143
SmiMI CAYNNNNRTG 1 cut(s) 1044
SmlI CTYRAG 2 cut(s) 260, 560
SmoI CTYRAG 2 cut(s) 260, 560
SphI GCATGC 1 cut(s) 1226
Sse9I AATT 6 cut(s) 270, 368, 547, 1042, 1329, 1333
SsiI CCGC 6 cut(s) 76, 104, 107, 429, 706, 842
SspMI CTAG 1 cut(s) 1268
StyD4I CCNGG 5 cut(s) 280, 416, 787, 809, 1240
StyI CCWWGG 2 cut(s) 171, 1307
TaaI ACNGT 3 cut(s) 142, 645, 763
TaiI ACGT 2 cut(s) 907, 913
TaqI TCGA 4 cut(s) 144, 1141, 1161, 1204
TasI AATT 6 cut(s) 270, 368, 547, 1042, 1329, 1333
TatI WGTACW 1 cut(s) 1285
TauI GCSGC 1 cut(s) 107
TfiI GAWTC 7 cut(s) 167, 193, 293, 653, 806, 1209, 1257
Tru1I TTAA 4 cut(s) 261, 921, 969, 1332
Tru9I TTAA 4 cut(s) 261, 921, 969, 1332
TscAI CASTG 6 cut(s) 86, 558, 594, 631, 894, 953
TseFI GTSAC 3 cut(s) 96, 754, 900
TseI GCWGC 1 cut(s) 35
Tsp45I GTSAC 3 cut(s) 96, 754, 900
TspDTI ATGAA 5 cut(s) 17, 381, 629, 1083, 1115
TspGWI ACGGA 2 cut(s) 201, 1206
TspRI CASTG 6 cut(s) 86, 558, 594, 631, 894, 953
Tth111I GACNNNGTC 1 cut(s) 898
Van91I CCANNNNNTGG 1 cut(s) 602
Vha464I CTTAAG 1 cut(s) 260
VneI GTGCAC 1 cut(s) 1005
VspI ATTAAT 1 cut(s) 1332
XagI CCTNNNNNAGG 1 cut(s) 745
XapI RAATTY 1 cut(s) 368
XceI RCATGY 2 cut(s) 1168, 1226
XcmI CCANNNNNNNNNTGG 2 cut(s) 474, 894
XmiI GTMKAC 1 cut(s) 144
XspI CTAG 1 cut(s) 1268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.