Rorug01G0044700

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0007277
Physical Location & Seq
Reverse (-)
7607651 .. 7608313
663 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0044700.1

Sequence Viewer

Length: 663 bp
ATGATGTCTTCGCGATCGCGTGAAAGAACAGTTCACCCGGAGTGCAAGAATTCTTCCAATCTCTACCATGAATGCCGTGAATATTGCTTCAAAGCTATTTTAGAAGCCAGAGATGGGATGGATCTAAATCAACTACTTGAATCTGTACAAGATGTTAGTGGCAACTCAAACTTAGAGGCTGCTTCTCAGCGAGAGAAAGAAGATGCTGTTAGTGGTGTTGACCACACAAAGTTGACAGGGAGGAAGAAAAAATTGAAGGAATTGCAGAGGAAGGTGACAGAGGCTATAGATAAGAATCAAACACAGGTCAAGGCCGAACAGAAAAGAATGGAGCCTCCAGAAGAATCGTCGAGGGGGATTTCGAAACAAAAATGGCTTGAAGAGAGGGAGAGAAAGGTGGGGAAACTCTTGGATGCAAATGGTTTGGATATGAAAAGAGCATACATGCTAGATACTCAAGAGATGGCCAAGGAAAAATACAAGAGATGGAAAAGGAGACCTGCACCATGTGGCTGGGATGTTTTTAATTCGAAGACGTTGTACAATGCACACAAGAAACGAACAAATAAAATCGAAGTTGACTTGGAAGAATACAATAAGATGAAACAGGAAGAAGCTTTCAGCTGCCTCGAATATGGAAAGAATCCGAAGGTGTCGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidohydro_3

Protein Analysis

220

Amino Acids

25.74

Weight (kDa)

9.12

Isoelectric Point (pI)

59.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SYF2 PF08231 140 - 205 3.6e-07 SYF2 splicing factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 508
AccII CGCG 2 cut(s) 13, 19
AclWI GGATC 1 cut(s) 129
AcoI YGGCCR 1 cut(s) 465
AcsI RAATTY 1 cut(s) 49
AdeI CACNNNGTG 1 cut(s) 509
AfaI GTAC 2 cut(s) 147, 542
AfiI CCNNNNNNNGG 1 cut(s) 114
AgsI TTSAA 4 cut(s) 91, 140, 256, 380
AluBI AGCT 3 cut(s) 95, 617, 624
AluI AGCT 3 cut(s) 95, 617, 624
Alw26I GTCTC 1 cut(s) 490
AlwI GGATC 1 cut(s) 129
AoxI GGCC 2 cut(s) 312, 465
ApeKI GCWGC 2 cut(s) 179, 624
ApoI RAATTY 1 cut(s) 49
AsiSI GCGATCGC 1 cut(s) 17
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 2 cut(s) 26, 286
AsuII TTCGAA 2 cut(s) 362, 530
BalI TGGCCA 1 cut(s) 467
BarI GAAGNNNNNNTAC 2 cut(s) 524, 556
BbsI GAAGAC 1 cut(s) 539
BbvI GCAGC 2 cut(s) 166, 611
BccI CCATC 4 cut(s) 107, 112, 457, 480
BceAI ACGGC 1 cut(s) 60
BcnI CCSGG 1 cut(s) 38
BcoDI GTCTC 1 cut(s) 490
BfaI CTAG 1 cut(s) 449
BfmI CTRYAG 1 cut(s) 285
BfuAI ACCTGC 1 cut(s) 508
BisI GCNGC 2 cut(s) 180, 625
BlsI GCNGC 2 cut(s) 181, 626
Bme1390I CCNGG 1 cut(s) 38
BmiI GGNNCC 1 cut(s) 333
BmrFI CCNGG 1 cut(s) 38
BmsI GCATC 2 cut(s) 193, 403
BpiI GAAGAC 1 cut(s) 539
BpmI CTGGAG 1 cut(s) 321
Bpu14I TTCGAA 2 cut(s) 362, 530
BpuEI CTTGAG 1 cut(s) 441
BpuMI CCSGG 1 cut(s) 38
BsaBI GATNNNNATC 2 cut(s) 126, 294
BsaI GGTCTC 1 cut(s) 490
BsaJI CCNNGG 1 cut(s) 468
Bsc4I CCNNNNNNNGG 1 cut(s) 114
Bse8I GATNNNNATC 2 cut(s) 126, 294
BseDI CCNNGG 1 cut(s) 468
BseGI GGATG 3 cut(s) 123, 418, 523
BseJI GATNNNNATC 2 cut(s) 126, 294
BseLI CCNNNNNNNGG 1 cut(s) 114
BseMII CTCAG 1 cut(s) 200
BseXI GCAGC 2 cut(s) 166, 611
BseYI CCCAGC 1 cut(s) 513
BsgI GTGCAG 1 cut(s) 486
Bsh1236I CGCG 2 cut(s) 13, 19
Bsh1285I CGRYCG 1 cut(s) 17
BshFI GGCC 2 cut(s) 314, 467
BsiEI CGRYCG 1 cut(s) 17
BsiSI CCGG 1 cut(s) 38
BslI CCNNNNNNNGG 1 cut(s) 114
BsmAI GTCTC 1 cut(s) 490
BsmI GAATGC 1 cut(s) 77
BsnI GGCC 2 cut(s) 314, 467
Bso31I GGTCTC 1 cut(s) 490
Bsp119I TTCGAA 2 cut(s) 362, 530
Bsp1407I TGTACA 2 cut(s) 145, 540
Bsp143I GATC 2 cut(s) 14, 121
Bsp68I TCGCGA 1 cut(s) 13
BspANI GGCC 2 cut(s) 314, 467
BspCNI CTCAG 1 cut(s) 199
BspFNI CGCG 2 cut(s) 13, 19
BspLI GGNNCC 1 cut(s) 333
BspMI ACCTGC 1 cut(s) 508
BspPI GGATC 1 cut(s) 129
BspT104I TTCGAA 2 cut(s) 362, 530
BspTNI GGTCTC 1 cut(s) 490
BsrGI TGTACA 2 cut(s) 145, 540
BssECI CCNNGG 1 cut(s) 468
BssMI GATC 2 cut(s) 14, 121
BssT1I CCWWGG 1 cut(s) 468
Bst4CI ACNGT 1 cut(s) 31
Bst6I CTCTTC 1 cut(s) 375
BstAUI TGTACA 2 cut(s) 145, 540
BstBI TTCGAA 2 cut(s) 362, 530
BstDEI CTNAG 2 cut(s) 172, 186
BstF5I GGATG 3 cut(s) 123, 418, 523
BstFNI CGCG 2 cut(s) 13, 19
BstKTI GATC 2 cut(s) 17, 124
BstMAI GTCTC 1 cut(s) 490
BstMBI GATC 2 cut(s) 14, 121
BstMCI CGRYCG 1 cut(s) 17
BstNSI RCATGY 1 cut(s) 448
BstSCI CCNGG 1 cut(s) 36
BstSFI CTRYAG 1 cut(s) 285
BstUI CGCG 2 cut(s) 13, 19
BstV1I GCAGC 2 cut(s) 166, 611
BstV2I GAAGAC 1 cut(s) 539
BstX2I RGATCY 1 cut(s) 121
BstXI CCANNNNNNTGG 1 cut(s) 513
BstYI RGATCY 1 cut(s) 121
BsuRI GGCC 2 cut(s) 314, 467
BtsCI GGATG 3 cut(s) 123, 418, 523
BtuMI TCGCGA 1 cut(s) 13
BveI ACCTGC 1 cut(s) 508
Csp6I GTAC 2 cut(s) 146, 541
CviAII CATG 3 cut(s) 68, 445, 507
CviQI GTAC 2 cut(s) 146, 541
DdeI CTNAG 2 cut(s) 172, 186
DpnI GATC 2 cut(s) 16, 123
DpnII GATC 2 cut(s) 14, 121
DraIII CACNNNGTG 1 cut(s) 509
EaeI YGGCCR 1 cut(s) 465
Eam1104I CTCTTC 1 cut(s) 375
EarI CTCTTC 1 cut(s) 375
Eco130I CCWWGG 1 cut(s) 468
Eco31I GGTCTC 1 cut(s) 490
EcoRI GAATTC 1 cut(s) 49
EcoT14I CCWWGG 1 cut(s) 468
ErhI CCWWGG 1 cut(s) 468
FaeI CATG 3 cut(s) 71, 448, 510
FaiI YATR 7 cut(s) 69, 287, 431, 442, 446, 508, 636
FatI CATG 3 cut(s) 67, 444, 506
Fnu4HI GCNGC 2 cut(s) 180, 625
FokI GGATG 3 cut(s) 130, 425, 530
Fsp4HI GCNGC 2 cut(s) 180, 625
FspBI CTAG 1 cut(s) 449
GluI GCNGC 2 cut(s) 180, 625
GsaI CCCAGC 1 cut(s) 517
GsuI CTGGAG 1 cut(s) 321
HaeIII GGCC 2 cut(s) 314, 467
HapII CCGG 1 cut(s) 38
Hin1II CATG 3 cut(s) 71, 448, 510
HincII GTYRAC 3 cut(s) 220, 234, 580
HindII GTYRAC 3 cut(s) 220, 234, 580
HindIII AAGCTT 1 cut(s) 615
HinfI GANTC 4 cut(s) 140, 295, 344, 643
HpaII CCGG 1 cut(s) 38
HphI GGTGA 2 cut(s) 26, 286
Hpy166II GTNNAC 4 cut(s) 34, 220, 234, 580
Hpy188I TCNGA 2 cut(s) 648, 658
Hpy188III TCNNGA 3 cut(s) 12, 338, 458
Hpy8I GTNNAC 4 cut(s) 34, 220, 234, 580
Hpy99I CGWCG 1 cut(s) 352
HpyAV CCTTC 3 cut(s) 250, 265, 643
HpyCH4III ACNGT 1 cut(s) 31
HpyCH4IV ACGT 1 cut(s) 536
HpyCH4V TGCA 5 cut(s) 45, 265, 416, 503, 548
HpyF3I CTNAG 2 cut(s) 172, 186
HpySE526I ACGT 1 cut(s) 536
Hsp92II CATG 3 cut(s) 71, 448, 510
Kzo9I GATC 2 cut(s) 14, 121
LmnI GCTCC 1 cut(s) 331
LpnPI CCDG 8 cut(s) 51, 121, 222, 290, 351, 499, 513, 593
Lsp1109I GCAGC 2 cut(s) 166, 611
LweI GCATC 2 cut(s) 193, 403
MaeI CTAG 1 cut(s) 449
MaeII ACGT 1 cut(s) 536
MaeIII GTNAC 1 cut(s) 274
MalI GATC 2 cut(s) 16, 123
MboI GATC 2 cut(s) 14, 121
MboII GAAGA 8 cut(s) 45, 212, 256, 353, 392, 544, 599, 623
MflI RGATCY 1 cut(s) 121
MlsI TGGCCA 1 cut(s) 467
MluCI AATT 4 cut(s) 49, 251, 260, 526
MluNI TGGCCA 1 cut(s) 467
MmeI TCCRAC 1 cut(s) 636
MnlI CCTC 8 cut(s) 169, 234, 261, 274, 345, 345, 378, 638
Mox20I TGGCCA 1 cut(s) 467
MscI TGGCCA 1 cut(s) 467
MseI TTAA 1 cut(s) 525
Msp20I TGGCCA 1 cut(s) 467
MspA1I CMGCKG 1 cut(s) 624
MspI CCGG 1 cut(s) 38
MspR9I CCNGG 1 cut(s) 38
Mva1269I GAATGC 1 cut(s) 77
MvnI CGCG 2 cut(s) 13, 19
NciI CCSGG 1 cut(s) 38
NdeII GATC 2 cut(s) 14, 121
NlaIII CATG 3 cut(s) 71, 448, 510
NlaIV GGNNCC 1 cut(s) 333
NmuCI GTSAC 1 cut(s) 274
NruI TCGCGA 1 cut(s) 13
NspI RCATGY 1 cut(s) 448
NspV TTCGAA 2 cut(s) 362, 530
PctI GAATGC 1 cut(s) 77
PfeI GAWTC 4 cut(s) 140, 295, 344, 643
PkrI GCNGC 2 cut(s) 181, 626
Ple19I CGATCG 1 cut(s) 17
PspFI CCCAGC 1 cut(s) 513
PspN4I GGNNCC 1 cut(s) 333
PsuI RGATCY 1 cut(s) 121
PvuI CGATCG 1 cut(s) 17
PvuII CAGCTG 1 cut(s) 624
RgaI GCGATCGC 1 cut(s) 17
RruI TCGCGA 1 cut(s) 13
RsaI GTAC 2 cut(s) 147, 542
RsaNI GTAC 2 cut(s) 146, 541
SaqAI TTAA 1 cut(s) 525
SatI GCNGC 2 cut(s) 180, 625
Sau3AI GATC 2 cut(s) 14, 121
ScrFI CCNGG 1 cut(s) 38
SetI ASST 9 cut(s) 97, 276, 309, 399, 502, 539, 619, 626, 654
SfaAI GCGATCGC 1 cut(s) 17
SfaNI GCATC 2 cut(s) 193, 403
SfcI CTRYAG 1 cut(s) 285
SfuI TTCGAA 2 cut(s) 362, 530
SgfI GCGATCGC 1 cut(s) 17
SmlI CTYRAG 1 cut(s) 456
SmoI CTYRAG 1 cut(s) 456
Sse9I AATT 4 cut(s) 49, 251, 260, 526
SspI AATATT 1 cut(s) 83
SspMI CTAG 1 cut(s) 449
StyD4I CCNGG 1 cut(s) 36
StyI CCWWGG 1 cut(s) 468
TaaI ACNGT 1 cut(s) 31
TaiI ACGT 1 cut(s) 539
TaqI TCGA 5 cut(s) 350, 362, 530, 573, 630
TasI AATT 4 cut(s) 49, 251, 260, 526
TatI WGTACW 2 cut(s) 145, 540
TfiI GAWTC 4 cut(s) 140, 295, 344, 643
Tru1I TTAA 1 cut(s) 525
Tru9I TTAA 1 cut(s) 525
TseFI GTSAC 1 cut(s) 274
TseI GCWGC 2 cut(s) 179, 624
Tsp45I GTSAC 1 cut(s) 274
TspDTI ATGAA 3 cut(s) 84, 446, 617
XapI RAATTY 1 cut(s) 49
XceI RCATGY 1 cut(s) 448
XcmI CCANNNNNNNNNTGG 1 cut(s) 115
XspI CTAG 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.