Rh4AG012000

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0007277
Physical Location & Seq
Forward (+)
1989174 .. 1991450
2277 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG012000.1

Sequence Viewer

Length: 639 bp
ATGAAGAGCTGCAATGTGTTTTCCAAAAGTTTTATGGAATTTGTCCAGCTTGAATTTGGCGAAGAGATTTCACCAGCCCTTGGAGATCTGGGAAACTTGGAATCCATGAATTTGAAGAACAATCAGTTGACTGGTCCTATCGCTACAACTCTTACCCAAATTCCAAACCTTAGAACTCTGTTTACAGCTAAGCTTCATTTCAGCGTTGCTAGTATCTGCCAATCAAAGGATATGGTAGGGTACGGGACTAAAGAATTAGATCTTAAGGGAAAAATTGTAGTCCCTGGATTTATTGATTCCCATGTGCATCTGATCTTTGGTGGACTGCAGATGATGCGTGTAGAACTACATGGCATAAACTCAAAAGATGAATTTGTGAAGAGGATCAAAGAAGCTGTGAGAAGTCAGTTCCCTGCCTTTTTATTTTTCTCGCAATTTTCTCAAACCTATGCAAACACAGTGCATGTGAATGTAGAATGTGTAAATTTTTCTTTTTATTTTTTTATTTATTTTTTACACTCTTTGTTTATGTATTTATTCTTTTGGGATAAGAAACATTCTTTTATCAAAAGATCAAGCAAAGTATATCACAGGGACAAGATGTTTGTGTTATATGGTCTGGTCTACATCCCAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidohydro_3

Protein Analysis

212

Amino Acids

24.54

Weight (kDa)

9.17

Isoelectric Point (pI)

31.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_3 PF07969 85 - 134 3.4e-10 Amidohydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 80
AccI GTMKAC 1 cut(s) 624
AclWI GGATC 1 cut(s) 392
AcsI RAATTY 6 cut(s) 38, 53, 109, 159, 371, 484
AfaI GTAC 1 cut(s) 242
AfiI CCNNNNNNNGG 2 cut(s) 80, 226
AflII CTTAAG 1 cut(s) 263
AgsI TTSAA 2 cut(s) 53, 115
AjnI CCWGG 1 cut(s) 283
AluBI AGCT 5 cut(s) 9, 49, 188, 193, 395
AluI AGCT 5 cut(s) 9, 49, 188, 193, 395
AlwI GGATC 1 cut(s) 392
ApeKI GCWGC 1 cut(s) 9
ApoI RAATTY 6 cut(s) 38, 53, 109, 159, 371, 484
ArsI GACNNNNNNTTYG 2 cut(s) 587, 619
AspS9I GGNCC 1 cut(s) 134
AsuHPI GGTGA 1 cut(s) 63
AvaII GGWCC 1 cut(s) 134
BciT130I CCWGG 1 cut(s) 285
BfaI CTAG 1 cut(s) 210
BfmI CTRYAG 1 cut(s) 326
BfrI CTTAAG 1 cut(s) 263
BglII AGATCT 2 cut(s) 85, 259
BisI GCNGC 1 cut(s) 10
BlpI GCTNAGC 1 cut(s) 189
BlsI GCNGC 1 cut(s) 11
Bme1390I CCNGG 1 cut(s) 285
Bme18I GGWCC 1 cut(s) 134
BmgT120I GGNCC 1 cut(s) 134
BmrFI CCNGG 1 cut(s) 285
BmsI GCATC 2 cut(s) 316, 324
Bpu1102I GCTNAGC 1 cut(s) 189
BsaJI CCNNGG 2 cut(s) 79, 283
Bsc4I CCNNNNNNNGG 2 cut(s) 80, 226
Bse1I ACTGG 1 cut(s) 136
Bse3DI GCAATG 1 cut(s) 19
BseBI CCWGG 1 cut(s) 285
BseDI CCNNGG 2 cut(s) 79, 283
BseGI GGATG 1 cut(s) 627
BseLI CCNNNNNNNGG 2 cut(s) 80, 226
BseMI GCAATG 1 cut(s) 19
BseNI ACTGG 1 cut(s) 136
BslFI GGGAC 3 cut(s) 259, 266, 608
BslI CCNNNNNNNGG 2 cut(s) 80, 226
BsmFI GGGAC 3 cut(s) 259, 266, 608
Bsp143I GATC 5 cut(s) 85, 259, 312, 384, 572
Bsp1720I GCTNAGC 1 cut(s) 189
BspMAI CTGCAG 1 cut(s) 330
BspPI GGATC 1 cut(s) 392
BspTI CTTAAG 1 cut(s) 263
BsrDI GCAATG 1 cut(s) 19
BsrI ACTGG 1 cut(s) 136
BssECI CCNNGG 2 cut(s) 79, 283
BssMI GATC 5 cut(s) 85, 259, 312, 384, 572
BssT1I CCWWGG 1 cut(s) 79
Bst2UI CCWGG 1 cut(s) 285
Bst4CI ACNGT 1 cut(s) 460
Bst6I CTCTTC 2 cut(s) 57, 374
BstAFI CTTAAG 1 cut(s) 263
BstAPI GCANNNNNTGC 1 cut(s) 334
BstDEI CTNAG 2 cut(s) 170, 189
BstF5I GGATG 1 cut(s) 627
BstKTI GATC 5 cut(s) 88, 262, 315, 387, 575
BstMBI GATC 5 cut(s) 85, 259, 312, 384, 572
BstMWI GCNNNNNNNGC 1 cut(s) 334
BstNI CCWGG 1 cut(s) 285
BstNSI RCATGY 1 cut(s) 467
BstSCI CCNGG 1 cut(s) 283
BstSFI CTRYAG 1 cut(s) 326
BstX2I RGATCY 2 cut(s) 85, 259
BstYI RGATCY 2 cut(s) 85, 259
BtsCI GGATG 1 cut(s) 627
BtsIMutI CAGTG 1 cut(s) 465
Cfr13I GGNCC 1 cut(s) 134
Csp6I GTAC 1 cut(s) 241
CviAII CATG 4 cut(s) 106, 302, 350, 464
CviJI RGCY 6 cut(s) 9, 49, 77, 188, 193, 395
CviKI_1 RGCY 6 cut(s) 9, 49, 77, 188, 193, 395
CviQI GTAC 1 cut(s) 241
DdeI CTNAG 2 cut(s) 170, 189
DpnI GATC 5 cut(s) 87, 261, 314, 386, 574
DpnII GATC 5 cut(s) 85, 259, 312, 384, 572
Eam1104I CTCTTC 2 cut(s) 57, 374
EarI CTCTTC 2 cut(s) 57, 374
Eco130I CCWWGG 1 cut(s) 79
Eco47I GGWCC 1 cut(s) 134
EcoRII CCWGG 1 cut(s) 283
EcoT14I CCWWGG 1 cut(s) 79
ErhI CCWWGG 1 cut(s) 79
FaeI CATG 4 cut(s) 109, 305, 353, 467
FaqI GGGAC 3 cut(s) 259, 266, 608
FatI CATG 4 cut(s) 105, 301, 349, 463
FblI GTMKAC 1 cut(s) 624
Fnu4HI GCNGC 1 cut(s) 10
FokI GGATG 1 cut(s) 614
Fsp4HI GCNGC 1 cut(s) 10
FspBI CTAG 1 cut(s) 210
GluI GCNGC 1 cut(s) 10
Hin1II CATG 4 cut(s) 109, 305, 353, 467
HincII GTYRAC 1 cut(s) 129
HindII GTYRAC 1 cut(s) 129
HindIII AAGCTT 1 cut(s) 191
HinfI GANTC 2 cut(s) 101, 296
HphI GGTGA 1 cut(s) 63
Hpy166II GTNNAC 4 cut(s) 129, 183, 323, 625
Hpy188I TCNGA 1 cut(s) 312
Hpy8I GTNNAC 4 cut(s) 129, 183, 323, 625
HpyCH4III ACNGT 1 cut(s) 460
HpyCH4V TGCA 5 cut(s) 12, 307, 328, 452, 463
HpyF10VI GCNNNNNNNGC 1 cut(s) 334
HpyF3I CTNAG 2 cut(s) 170, 189
Hsp92II CATG 4 cut(s) 109, 305, 353, 467
Kzo9I GATC 5 cut(s) 85, 259, 312, 384, 572
LpnPI CCDG 9 cut(s) 59, 74, 87, 117, 270, 297, 426, 577, 605
LweI GCATC 2 cut(s) 316, 324
MaeI CTAG 1 cut(s) 210
MalI GATC 5 cut(s) 87, 261, 314, 386, 574
MboI GATC 5 cut(s) 85, 259, 312, 384, 572
MboII GAAGA 4 cut(s) 16, 74, 127, 391
MflI RGATCY 2 cut(s) 85, 259
MluCI AATT 9 cut(s) 38, 53, 109, 159, 254, 273, 371, 434, 484
MnlI CCTC 1 cut(s) 375
MseI TTAA 1 cut(s) 264
MslI CAYNNNNRTG 1 cut(s) 468
MspCI CTTAAG 1 cut(s) 263
MspR9I CCNGG 1 cut(s) 285
MvaI CCWGG 1 cut(s) 285
MwoI GCNNNNNNNGC 1 cut(s) 334
NdeII GATC 5 cut(s) 85, 259, 312, 384, 572
NlaIII CATG 4 cut(s) 109, 305, 353, 467
NspI RCATGY 1 cut(s) 467
PfeI GAWTC 2 cut(s) 101, 296
PflMI CCANNNNNTGG 1 cut(s) 80
PkrI GCNGC 1 cut(s) 11
Psp6I CCWGG 1 cut(s) 283
PspGI CCWGG 1 cut(s) 283
PspPI GGNCC 1 cut(s) 134
PsrI GAACNNNNNNTAC 2 cut(s) 166, 198
PstI CTGCAG 1 cut(s) 330
PsuI RGATCY 2 cut(s) 85, 259
RsaI GTAC 1 cut(s) 242
RsaNI GTAC 1 cut(s) 241
RseI CAYNNNNRTG 1 cut(s) 468
SaqAI TTAA 1 cut(s) 264
SatI GCNGC 1 cut(s) 10
Sau3AI GATC 5 cut(s) 85, 259, 312, 384, 572
Sau96I GGNCC 1 cut(s) 134
ScrFI CCNGG 1 cut(s) 285
SetI ASST 7 cut(s) 11, 51, 171, 190, 195, 397, 449
SfaNI GCATC 2 cut(s) 316, 324
SfcI CTRYAG 1 cut(s) 326
SinI GGWCC 1 cut(s) 134
SmiMI CAYNNNNRTG 1 cut(s) 468
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
Sse9I AATT 9 cut(s) 38, 53, 109, 159, 254, 273, 371, 434, 484
SspMI CTAG 1 cut(s) 210
StyD4I CCNGG 1 cut(s) 283
StyI CCWWGG 1 cut(s) 79
TaaI ACNGT 1 cut(s) 460
TasI AATT 9 cut(s) 38, 53, 109, 159, 254, 273, 371, 434, 484
TfiI GAWTC 2 cut(s) 101, 296
Tru1I TTAA 1 cut(s) 264
Tru9I TTAA 1 cut(s) 264
TscAI CASTG 1 cut(s) 465
TseI GCWGC 1 cut(s) 9
TspDTI ATGAA 4 cut(s) 17, 122, 185, 384
TspRI CASTG 1 cut(s) 465
Van91I CCANNNNNTGG 1 cut(s) 80
Vha464I CTTAAG 1 cut(s) 263
VpaK11BI GGWCC 1 cut(s) 134
XapI RAATTY 6 cut(s) 38, 53, 109, 159, 371, 484
XceI RCATGY 1 cut(s) 467
XcmI CCANNNNNNNNNTGG 2 cut(s) 31, 53
XmiI GTMKAC 1 cut(s) 624
XspI CTAG 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.