Rorug01G0044600

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0007277
Physical Location & Seq
Reverse (-)
7601119 .. 7601982
864 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0044600.1

Sequence Viewer

Length: 864 bp
ATGTCTTCTAGTGAAAGAGCAGTGCACTCGGAGTGCAAGAATTCTTCCAATCTCTACCATGAATGCCGTGAATATTGCTTCAAAGCTATTTTAGAAGCCAGAGTTGGGATGGATCTAAATCAACTACGTGAATCTGTACAAGATGTTAGTGGCAACTCAAACTTAGAGGCTTCTTCTCAGCGAGAGAAAGAAGATGCTGTTAATGGTGTTGACCACACAAAGTTGACAGGGAGGAAGAAAAAATTGAAGGAATTGCAGAGGAAGGTGACAGAGGCTATAGATAAGAATCAAACACAGGTTAAAGCCGAACAGAAAAGAATGGAGCCTCCAGAAGAATCGTTGAGGGGGATTTCGAAACAAAAATGGCTTGAAGAGAGGGAGAGAAAGGTGGGGAAACTCTTGGATGCAAATGGTTTGGATTTGAAAAGAGCATACATGCTAGATACACAAGAGATGGCCGAGGCAAAATACAAGAGATGGAAAAAGAAACCTGCACCATGTGGCTGGGATGTTTTTAATTCGAAAACATTGTACAATGCACACAAAAAACGAACAAACAAGATCGAAGTTGACATGGAAGAATACAATAAGATGAAACAGGAAGGAGCTTTTAGCTGCCTCGAAAATGGAAAGACTCCGAAGGTCTCGGAGGAGAAAGTTGACAAGATGTTGCAGGAGCTCAAGGAACTTGAAGAGAAGAAAAAGAAATTTAGCAGGAGGAGGAGGTTCCATGAGGACAAGGATATTGACTCGATCAATGACCGCAATGAGCATTTCAACAAGAAGATTGAGAGAGCCTTTGGTAAGCACACATTGGAAATCAAAAACAATCTCCAGCGAGGTAAGGTGCATCTGAGTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidohydro_3

Protein Analysis

287

Amino Acids

33.69

Weight (kDa)

9.33

Isoelectric Point (pI)

54.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SYF2 PF08231 137 - 278 3.7e-33 SYF2 splicing factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 499
AciI CCGC 1 cut(s) 763
AclWI GGATC 1 cut(s) 120
AcoI YGGCCR 1 cut(s) 456
AcsI RAATTY 2 cut(s) 40, 707
AdeI CACNNNGTG 1 cut(s) 500
AfaI GTAC 2 cut(s) 138, 533
AfiI CCNNNNNNNGG 1 cut(s) 105
AgsI TTSAA 6 cut(s) 82, 247, 371, 424, 692, 778
AjuI GAANNNNNNNTTGG 2 cut(s) 87, 119
AluBI AGCT 4 cut(s) 86, 608, 615, 679
AluI AGCT 4 cut(s) 86, 608, 615, 679
Alw21I GWGCWC 2 cut(s) 27, 681
Alw26I GTCTC 1 cut(s) 649
Alw44I GTGCAC 1 cut(s) 23
AlwI GGATC 1 cut(s) 120
AoxI GGCC 1 cut(s) 456
ApaLI GTGCAC 1 cut(s) 23
ApeKI GCWGC 1 cut(s) 615
ApoI RAATTY 2 cut(s) 40, 707
AsuHPI GGTGA 1 cut(s) 277
AsuII TTCGAA 2 cut(s) 353, 521
BaeGI GKGCMC 1 cut(s) 27
BanII GRGCYC 1 cut(s) 681
Bbv12I GWGCWC 2 cut(s) 27, 681
BbvI GCAGC 1 cut(s) 602
BccI CCATC 3 cut(s) 103, 448, 471
BceAI ACGGC 1 cut(s) 51
BcgI CGANNNNNNTGC 2 cut(s) 9, 43
BcoDI GTCTC 1 cut(s) 649
BfaI CTAG 2 cut(s) 9, 440
BfmI CTRYAG 1 cut(s) 276
BfuAI ACCTGC 1 cut(s) 499
BisI GCNGC 1 cut(s) 616
BlsI GCNGC 1 cut(s) 617
BmiI GGNNCC 2 cut(s) 324, 728
BmsI GCATC 3 cut(s) 184, 394, 859
BpmI CTGGAG 2 cut(s) 312, 818
Bpu14I TTCGAA 2 cut(s) 353, 521
BpuEI CTTGAG 1 cut(s) 665
BsaAI YACGTR 1 cut(s) 128
BsaBI GATNNNNATC 2 cut(s) 117, 285
BsaI GGTCTC 1 cut(s) 649
BsaJI CCNNGG 1 cut(s) 459
Bsc4I CCNNNNNNNGG 1 cut(s) 105
Bse3DI GCAATG 1 cut(s) 772
Bse8I GATNNNNATC 2 cut(s) 117, 285
BseDI CCNNGG 1 cut(s) 459
BseGI GGATG 3 cut(s) 114, 409, 514
BseJI GATNNNNATC 2 cut(s) 117, 285
BseLI CCNNNNNNNGG 1 cut(s) 105
BseMI GCAATG 1 cut(s) 772
BseMII CTCAG 2 cut(s) 191, 845
BseRI GAGGAG 3 cut(s) 665, 733, 736
BseSI GKGCMC 1 cut(s) 27
BseXI GCAGC 1 cut(s) 602
BseYI CCCAGC 1 cut(s) 504
BsgI GTGCAG 1 cut(s) 477
BshFI GGCC 1 cut(s) 458
BsiHKAI GWGCWC 2 cut(s) 27, 681
BslI CCNNNNNNNGG 1 cut(s) 105
BsmAI GTCTC 1 cut(s) 649
BsmI GAATGC 1 cut(s) 68
BsnI GGCC 1 cut(s) 458
Bso31I GGTCTC 1 cut(s) 649
Bsp119I TTCGAA 2 cut(s) 353, 521
Bsp1286I GDGCHC 2 cut(s) 27, 681
Bsp1407I TGTACA 2 cut(s) 136, 531
Bsp143I GATC 3 cut(s) 112, 561, 753
BspACI CCGC 1 cut(s) 763
BspANI GGCC 1 cut(s) 458
BspCNI CTCAG 2 cut(s) 190, 846
BspLI GGNNCC 2 cut(s) 324, 728
BspMI ACCTGC 1 cut(s) 499
BspPI GGATC 1 cut(s) 120
BspT104I TTCGAA 2 cut(s) 353, 521
BspTNI GGTCTC 1 cut(s) 649
BsrDI GCAATG 1 cut(s) 772
BsrGI TGTACA 2 cut(s) 136, 531
BssECI CCNNGG 1 cut(s) 459
BssMI GATC 3 cut(s) 112, 561, 753
Bst6I CTCTTC 2 cut(s) 366, 687
BstAUI TGTACA 2 cut(s) 136, 531
BstBAI YACGTR 1 cut(s) 128
BstBI TTCGAA 2 cut(s) 353, 521
BstDEI CTNAG 3 cut(s) 163, 177, 854
BstF5I GGATG 3 cut(s) 114, 409, 514
BstKTI GATC 3 cut(s) 115, 564, 756
BstMAI GTCTC 1 cut(s) 649
BstMBI GATC 3 cut(s) 112, 561, 753
BstNSI RCATGY 1 cut(s) 439
BstSFI CTRYAG 1 cut(s) 276
BstSLI GKGCMC 1 cut(s) 27
BstV1I GCAGC 1 cut(s) 602
BstX2I RGATCY 1 cut(s) 112
BstXI CCANNNNNNTGG 1 cut(s) 504
BstYI RGATCY 1 cut(s) 112
BsuRI GGCC 1 cut(s) 458
BtsCI GGATG 3 cut(s) 114, 409, 514
BtsI GCAGTG 1 cut(s) 27
BtsIMutI CAGTG 1 cut(s) 27
BveI ACCTGC 1 cut(s) 499
Csp6I GTAC 2 cut(s) 137, 532
CviAII CATG 5 cut(s) 59, 436, 498, 574, 731
CviQI GTAC 2 cut(s) 137, 532
DdeI CTNAG 3 cut(s) 163, 177, 854
DpnI GATC 3 cut(s) 114, 563, 755
DpnII GATC 3 cut(s) 112, 561, 753
DraIII CACNNNGTG 1 cut(s) 500
EaeI YGGCCR 1 cut(s) 456
Eam1104I CTCTTC 2 cut(s) 366, 687
EarI CTCTTC 2 cut(s) 366, 687
Ecl136II GAGCTC 1 cut(s) 679
Eco24I GRGCYC 1 cut(s) 681
Eco31I GGTCTC 1 cut(s) 649
Eco53kI GAGCTC 1 cut(s) 679
EcoICRI GAGCTC 1 cut(s) 679
EcoRI GAATTC 1 cut(s) 40
EcoT38I GRGCYC 1 cut(s) 681
FaeI CATG 5 cut(s) 62, 439, 501, 577, 734
FaiI YATR 7 cut(s) 60, 278, 433, 437, 499, 575, 732
FatI CATG 5 cut(s) 58, 435, 497, 573, 730
Fnu4HI GCNGC 1 cut(s) 616
FokI GGATG 3 cut(s) 121, 416, 521
FriOI GRGCYC 1 cut(s) 681
Fsp4HI GCNGC 1 cut(s) 616
FspBI CTAG 2 cut(s) 9, 440
GluI GCNGC 1 cut(s) 616
GsaI CCCAGC 1 cut(s) 508
GsuI CTGGAG 2 cut(s) 312, 818
HaeIII GGCC 1 cut(s) 458
Hin1II CATG 5 cut(s) 62, 439, 501, 577, 734
HincII GTYRAC 4 cut(s) 211, 225, 571, 661
HindII GTYRAC 4 cut(s) 211, 225, 571, 661
HinfI GANTC 5 cut(s) 131, 286, 335, 634, 749
HphI GGTGA 1 cut(s) 277
Hpy166II GTNNAC 5 cut(s) 25, 211, 225, 571, 661
Hpy188I TCNGA 4 cut(s) 31, 639, 649, 855
Hpy188III TCNNGA 1 cut(s) 329
Hpy8I GTNNAC 5 cut(s) 25, 211, 225, 571, 661
HpyAV CCTTC 4 cut(s) 241, 256, 596, 634
HpyCH4IV ACGT 1 cut(s) 127
HpyCH4V TGCA 8 cut(s) 25, 36, 256, 407, 494, 539, 673, 850
HpyF3I CTNAG 3 cut(s) 163, 177, 854
HpySE526I ACGT 1 cut(s) 127
Hsp92II CATG 5 cut(s) 62, 439, 501, 577, 734
Kzo9I GATC 3 cut(s) 112, 561, 753
LmnI GCTCC 3 cut(s) 322, 605, 676
Lsp1109I GCAGC 1 cut(s) 602
LweI GCATC 3 cut(s) 184, 394, 859
MaeI CTAG 2 cut(s) 9, 440
MaeII ACGT 1 cut(s) 127
MaeIII GTNAC 2 cut(s) 265, 857
MalI GATC 3 cut(s) 114, 563, 755
MboI GATC 3 cut(s) 112, 561, 753
MflI RGATCY 1 cut(s) 112
MhlI GDGCHC 2 cut(s) 27, 681
MluCI AATT 5 cut(s) 40, 242, 251, 517, 707
MlyI GAGTC 2 cut(s) 628, 743
MseI TTAA 3 cut(s) 201, 300, 516
MslI CAYNNNNRTG 1 cut(s) 855
Mva1269I GAATGC 1 cut(s) 68
NdeII GATC 3 cut(s) 112, 561, 753
NlaIII CATG 5 cut(s) 62, 439, 501, 577, 734
NlaIV GGNNCC 2 cut(s) 324, 728
NmeAIII GCCGAG 1 cut(s) 484
NmuCI GTSAC 2 cut(s) 265, 857
NspI RCATGY 1 cut(s) 439
NspV TTCGAA 2 cut(s) 353, 521
PctI GAATGC 1 cut(s) 68
PfeI GAWTC 3 cut(s) 131, 286, 335
PkrI GCNGC 1 cut(s) 617
PleI GAGTC 2 cut(s) 628, 743
PpsI GAGTC 2 cut(s) 628, 743
Ppu21I YACGTR 1 cut(s) 128
Psp124BI GAGCTC 1 cut(s) 681
PspFI CCCAGC 1 cut(s) 504
PspN4I GGNNCC 2 cut(s) 324, 728
PsuI RGATCY 1 cut(s) 112
RsaI GTAC 2 cut(s) 138, 533
RsaNI GTAC 2 cut(s) 137, 532
RseI CAYNNNNRTG 1 cut(s) 855
SacI GAGCTC 1 cut(s) 681
SaqAI TTAA 3 cut(s) 201, 300, 516
SatI GCNGC 1 cut(s) 616
Sau3AI GATC 3 cut(s) 112, 561, 753
SchI GAGTC 2 cut(s) 628, 743
SduI GDGCHC 2 cut(s) 27, 681
SfaNI GCATC 3 cut(s) 184, 394, 859
SfcI CTRYAG 1 cut(s) 276
SfuI TTCGAA 2 cut(s) 353, 521
SmiMI CAYNNNNRTG 1 cut(s) 855
SmlI CTYRAG 1 cut(s) 680
SmoI CTYRAG 1 cut(s) 680
Sse9I AATT 5 cut(s) 40, 242, 251, 517, 707
SsiI CCGC 1 cut(s) 763
SspI AATATT 1 cut(s) 74
SspMI CTAG 2 cut(s) 9, 440
SstI GAGCTC 1 cut(s) 681
TaiI ACGT 1 cut(s) 130
TaqI TCGA 5 cut(s) 353, 521, 564, 621, 752
TasI AATT 5 cut(s) 40, 242, 251, 517, 707
TatI WGTACW 2 cut(s) 136, 531
TfiI GAWTC 3 cut(s) 131, 286, 335
Tru1I TTAA 3 cut(s) 201, 300, 516
Tru9I TTAA 3 cut(s) 201, 300, 516
TscAI CASTG 1 cut(s) 27
TseFI GTSAC 2 cut(s) 265, 857
TseI GCWGC 1 cut(s) 615
Tsp45I GTSAC 2 cut(s) 265, 857
TspDTI ATGAA 2 cut(s) 75, 608
TspRI CASTG 1 cut(s) 27
VneI GTGCAC 1 cut(s) 23
XapI RAATTY 2 cut(s) 40, 707
XceI RCATGY 1 cut(s) 439
XcmI CCANNNNNNNNNTGG 1 cut(s) 106
XspI CTAG 2 cut(s) 9, 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.