Rh1AG060700

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0007277
Physical Location & Seq
Forward (+)
9993989 .. 10003136
9148 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG060700.1

Sequence Viewer

Length: 1233 bp
ATGAAGTCTGCTCTCCTACTCCTCTCAGCTTTTGCTGCTCTCCTCCTCGCCATTTTCGCTTCTCTCAATCCCAACCGCCAGTGGTTCAGTTGGAGGTCACCGGCGGCGGACTTGGTGGTGAAGAATGCCAAGATATACACCGTCGACGAGTCTCTCCCCTTCGCCGATTCCATGGCCGTTCGCAACGGAAGGATTCTCCGGATTGGAAGCTACTCCTCAGTTCAGGATATGGTAGGGTATGGGACTAAAGTGTTAGATCTTAAGGGAAAAATTGTAGTCCCTGGATTTATTGATTCCCATGTGCATCTGATCCTTAGTGGACTGCAGATGATGCGTGTAGAACTACATGGCATAAACTCAAAAGATGAATTTGTGAAGAGGATCAAAGAAGCTGTGAGAAATGCAAAACAAGGTTCCTGGATTTTGGGCGGTGGATGGAACAATGATCTATGGGGAGGTGAGTTGCCATTGGCTTCTTGGATTGACGATATTACACCTTATAATCCTGTCTTTCTATCAAGAATGGATGGGCATATGAGCTTGGCTAATTCAGTGGCACTCAAGTTGGGTGGGATTACTAAATCATCAGTGGATCCAAATGGTGGAACCATCATGAAAACCACCAGTGGAGAACCTACTGGACTGTTGATTGATTCTGCAATGGAACATATCCTTCCACTCATTCCAGAGGTCTCAGTAGAGGAGCGGAGGGAAGCTTTGCGTAAAGCCAGCAATCATGCCTTGATGAGGGGTGTGACGACAGTTGTTGACGTTGGTAGATACTTTCCTGGGACTTCTGTAAAAGATTCCTGGGAAGATTTTTCAGATGTGTATCAATGGGCTGATTTGTCAGGGAAGATGACGATCAGGGTCTGCTTATTTTTCCCACTGGCGACTTGGTCACGTTTACGTGATCTTATTAACAATGTTGGCCAATCTATGAGCCAGTGGATTTATTTGGGTGGGGTTAAAGCATTTGCTGATGGGTCATTGGGTTCTCGTAGCGCACTTTTCTATGAGCCATATGTTGATGAGCCTCATAATTATGGCTTACAAGTAGGAGATTATGAAAGTCTCCACAACCTGACCTTAGCATCAGATAGAGTTGGGCTTCAGGTTGCTATTCATGCTATAGGCGATCGAGCAAATGACTTGATCCTCGACATGTATGAGTCAGTCATTTCAAAAAACGGAGTGAGGGATCGAAGATTCAGGGTAAAAAAATTTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidohydro_3

Protein Analysis

410

Amino Acids

45.41

Weight (kDa)

7.82

Isoelectric Point (pI)

36.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_3 PF07969 83 - 405 5.2e-54 Amidohydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 501
AccB7I CCANNNNNTGG 1 cut(s) 602
AccBSI CCGCTC 1 cut(s) 706
AccI GTMKAC 1 cut(s) 144
AccIII TCCGGA 1 cut(s) 198
AciI CCGC 5 cut(s) 76, 104, 107, 429, 706
AclWI GGATC 6 cut(s) 304, 389, 587, 600, 1150, 1209
AcoI YGGCCR 2 cut(s) 174, 931
AcsI RAATTY 2 cut(s) 368, 1223
AcuI CTGAAG 1 cut(s) 1097
AfiI CCNNNNNNNGG 2 cut(s) 602, 747
AflII CTTAAG 1 cut(s) 260
AflIII ACRYGT 1 cut(s) 1164
AgsI TTSAA 1 cut(s) 1185
AjnI CCWGG 4 cut(s) 280, 416, 787, 809
AluBI AGCT 5 cut(s) 29, 210, 392, 540, 716
AluI AGCT 5 cut(s) 29, 210, 392, 540, 716
Alw26I GTCTC 3 cut(s) 156, 697, 1079
AlwI GGATC 6 cut(s) 304, 389, 587, 600, 1150, 1209
AlwNI CAGNNNCTG 1 cut(s) 873
Aor13HI TCCGGA 1 cut(s) 198
AoxI GGCC 2 cut(s) 174, 931
ApeKI GCWGC 1 cut(s) 35
ApoI RAATTY 2 cut(s) 368, 1223
AspLEI GCGC 1 cut(s) 1007
AsuHPI GGTGA 3 cut(s) 90, 130, 470
BalI TGGCCA 1 cut(s) 933
BamHI GGATCC 1 cut(s) 592
BbvI GCAGC 1 cut(s) 22
BccI CCATC 4 cut(s) 429, 521, 617, 977
BceAI ACGGC 1 cut(s) 161
BciT130I CCWGG 4 cut(s) 282, 418, 789, 811
BcoDI GTCTC 3 cut(s) 156, 697, 1079
BfmI CTRYAG 2 cut(s) 323, 1131
BfrI CTTAAG 1 cut(s) 260
BglII AGATCT 1 cut(s) 256
BisI GCNGC 2 cut(s) 36, 105
BlsI GCNGC 2 cut(s) 37, 106
Bme1390I CCNGG 4 cut(s) 282, 418, 789, 811
BmiI GGNNCC 3 cut(s) 415, 594, 607
BmrFI CCNGG 4 cut(s) 282, 418, 789, 811
BmsI GCATC 3 cut(s) 313, 321, 1103
Bpu10I CCTNAGC 1 cut(s) 1090
BpuEI CTTGAG 1 cut(s) 545
BsaAI YACGTR 1 cut(s) 911
BsaBI GATNNNNATC 2 cut(s) 831, 863
BsaI GGTCTC 1 cut(s) 697
BsaJI CCNNGG 4 cut(s) 171, 280, 788, 810
BsaWI WCCGGW 1 cut(s) 198
Bsc4I CCNNNNNNNGG 2 cut(s) 602, 747
Bse118I RCCGGY 1 cut(s) 100
Bse1I ACTGG 5 cut(s) 79, 624, 643, 894, 946
Bse3DI GCAATG 1 cut(s) 666
Bse8I GATNNNNATC 2 cut(s) 831, 863
BseAI TCCGGA 1 cut(s) 198
BseBI CCWGG 4 cut(s) 282, 418, 789, 811
BseDI CCNNGG 4 cut(s) 171, 280, 788, 810
BseGI GGATG 2 cut(s) 440, 532
BseJI GATNNNNATC 2 cut(s) 831, 863
BseLI CCNNNNNNNGG 2 cut(s) 602, 747
BseMI GCAATG 1 cut(s) 666
BseMII CTCAG 3 cut(s) 39, 231, 708
BseNI ACTGG 5 cut(s) 79, 624, 643, 894, 946
BseRI GAGGAG 5 cut(s) 11, 32, 35, 205, 716
BseXI GCAGC 1 cut(s) 22
Bsh1285I CGRYCG 1 cut(s) 1141
BshFI GGCC 2 cut(s) 176, 933
BsiEI CGRYCG 1 cut(s) 1141
BsiSI CCGG 2 cut(s) 101, 199
BslFI GGGAC 3 cut(s) 256, 263, 805
BslI CCNNNNNNNGG 2 cut(s) 602, 747
BsmAI GTCTC 3 cut(s) 156, 697, 1079
BsmFI GGGAC 3 cut(s) 256, 263, 805
BsmI GAATGC 1 cut(s) 130
BsnI GGCC 2 cut(s) 176, 933
Bso31I GGTCTC 1 cut(s) 697
Bsp13I TCCGGA 1 cut(s) 198
Bsp19I CCATGG 1 cut(s) 171
BspACI CCGC 5 cut(s) 76, 104, 107, 429, 706
BspANI GGCC 2 cut(s) 176, 933
BspCNI CTCAG 3 cut(s) 38, 230, 707
BspEI TCCGGA 1 cut(s) 198
BspHI TCATGA 1 cut(s) 612
BspLI GGNNCC 3 cut(s) 415, 594, 607
BspMAI CTGCAG 1 cut(s) 327
BspPI GGATC 6 cut(s) 304, 389, 587, 600, 1150, 1209
BspTI CTTAAG 1 cut(s) 260
BspTNI GGTCTC 1 cut(s) 697
BsrBI CCGCTC 1 cut(s) 706
BsrDI GCAATG 1 cut(s) 666
BsrFI RCCGGY 1 cut(s) 100
BsrI ACTGG 5 cut(s) 79, 624, 643, 894, 946
BssAI RCCGGY 1 cut(s) 100
BssECI CCNNGG 4 cut(s) 171, 280, 788, 810
BssT1I CCWWGG 1 cut(s) 171
Bst2UI CCWGG 4 cut(s) 282, 418, 789, 811
Bst4CI ACNGT 3 cut(s) 142, 645, 763
Bst6I CTCTTC 1 cut(s) 371
BstAFI CTTAAG 1 cut(s) 260
BstAPI GCANNNNNTGC 1 cut(s) 331
BstBAI YACGTR 1 cut(s) 911
BstC8I GCNNGC 1 cut(s) 730
BstDEI CTNAG 5 cut(s) 25, 217, 314, 694, 1090
BstDSI CCRYGG 1 cut(s) 171
BstEII GGTNACC 1 cut(s) 96
BstENI CCTNNNNNAGG 1 cut(s) 745
BstF5I GGATG 2 cut(s) 440, 532
BstHHI GCGC 1 cut(s) 1007
BstMAI GTCTC 3 cut(s) 156, 697, 1079
BstMCI CGRYCG 1 cut(s) 1141
BstMWI GCNNNNNNNGC 4 cut(s) 35, 56, 331, 1127
BstNI CCWGG 4 cut(s) 282, 418, 789, 811
BstNSI RCATGY 1 cut(s) 1168
BstPI GGTNACC 1 cut(s) 96
BstSCI CCNGG 4 cut(s) 280, 416, 787, 809
BstSFI CTRYAG 2 cut(s) 323, 1131
BstV1I GCAGC 1 cut(s) 22
BstX2I RGATCY 2 cut(s) 256, 592
BstYI RGATCY 2 cut(s) 256, 592
BsuRI GGCC 2 cut(s) 176, 933
BtgI CCRYGG 1 cut(s) 171
BtsCI GGATG 2 cut(s) 440, 532
BtsIMutI CAGTG 6 cut(s) 86, 558, 594, 631, 887, 953
Cac8I GCNNGC 1 cut(s) 730
CaiI CAGNNNCTG 1 cut(s) 873
CciI TCATGA 1 cut(s) 612
CfoI GCGC 1 cut(s) 1007
Cfr10I RCCGGY 1 cut(s) 100
CspCI CAANNNNNGTGG 2 cut(s) 550, 585
CviAII CATG 7 cut(s) 172, 299, 347, 613, 737, 1127, 1165
DdeI CTNAG 5 cut(s) 25, 217, 314, 694, 1090
EaeI YGGCCR 2 cut(s) 174, 931
Eam1104I CTCTTC 1 cut(s) 371
EarI CTCTTC 1 cut(s) 371
EciI GGCGGA 1 cut(s) 122
Eco130I CCWWGG 1 cut(s) 171
Eco31I GGTCTC 1 cut(s) 697
Eco57I CTGAAG 1 cut(s) 1097
Eco91I GGTNACC 1 cut(s) 96
EcoNI CCTNNNNNAGG 1 cut(s) 745
EcoO65I GGTNACC 1 cut(s) 96
EcoRII CCWGG 4 cut(s) 280, 416, 787, 809
EcoT14I CCWWGG 1 cut(s) 171
ErhI CCWWGG 1 cut(s) 171
FaeI CATG 7 cut(s) 175, 302, 350, 616, 740, 1130, 1168
FaqI GGGAC 3 cut(s) 256, 263, 805
FatI CATG 7 cut(s) 171, 298, 346, 612, 736, 1126, 1164
FauNDI CATATG 2 cut(s) 534, 1024
FblI GTMKAC 1 cut(s) 144
Fnu4HI GCNGC 2 cut(s) 36, 105
FokI GGATG 2 cut(s) 447, 539
Fsp4HI GCNGC 2 cut(s) 36, 105
GlaI GCGC 1 cut(s) 1006
GluI GCNGC 2 cut(s) 36, 105
HaeIII GGCC 2 cut(s) 176, 933
HapII CCGG 2 cut(s) 101, 199
HhaI GCGC 1 cut(s) 1007
Hin1II CATG 7 cut(s) 175, 302, 350, 616, 740, 1130, 1168
Hin6I GCGC 1 cut(s) 1005
HinP1I GCGC 1 cut(s) 1005
HincII GTYRAC 2 cut(s) 145, 769
HindII GTYRAC 2 cut(s) 145, 769
HindIII AAGCTT 1 cut(s) 714
HinfI GANTC 8 cut(s) 149, 167, 193, 293, 653, 806, 1172, 1209
HpaII CCGG 2 cut(s) 101, 199
HphI GGTGA 3 cut(s) 90, 130, 470
Hpy166II GTNNAC 4 cut(s) 145, 320, 769, 908
Hpy188I TCNGA 3 cut(s) 309, 826, 1099
Hpy188III TCNNGA 5 cut(s) 199, 224, 519, 613, 686
Hpy8I GTNNAC 4 cut(s) 145, 320, 769, 908
Hpy99I CGWCG 2 cut(s) 146, 149
HpyAV CCTTC 3 cut(s) 169, 183, 683
HpyCH4III ACNGT 3 cut(s) 142, 645, 763
HpyCH4IV ACGT 3 cut(s) 771, 904, 910
HpyCH4V TGCA 4 cut(s) 304, 325, 404, 659
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 56, 331, 1127
HpyF3I CTNAG 5 cut(s) 25, 217, 314, 694, 1090
HpySE526I ACGT 3 cut(s) 771, 904, 910
Hsp92II CATG 7 cut(s) 175, 302, 350, 616, 740, 1130, 1168
HspAI GCGC 1 cut(s) 1005
Kpn2I TCCGGA 1 cut(s) 198
LmnI GCTCC 1 cut(s) 703
Lsp1109I GCAGC 1 cut(s) 22
LweI GCATC 3 cut(s) 313, 321, 1103
MaeII ACGT 3 cut(s) 771, 904, 910
MaeIII GTNAC 3 cut(s) 96, 754, 900
MbiI CCGCTC 1 cut(s) 706
MboII GAAGA 5 cut(s) 133, 388, 827, 868, 1218
MflI RGATCY 2 cut(s) 256, 592
MlsI TGGCCA 1 cut(s) 933
MluCI AATT 5 cut(s) 270, 368, 547, 1042, 1223
MluNI TGGCCA 1 cut(s) 933
MlyI GAGTC 2 cut(s) 158, 1181
MmeI TCCRAC 1 cut(s) 71
Mox20I TGGCCA 1 cut(s) 933
MroI TCCGGA 1 cut(s) 198
MscI TGGCCA 1 cut(s) 933
MseI TTAA 3 cut(s) 261, 921, 969
MslI CAYNNNNRTG 1 cut(s) 1044
Msp20I TGGCCA 1 cut(s) 933
MspCI CTTAAG 1 cut(s) 260
MspI CCGG 2 cut(s) 101, 199
MspR9I CCNGG 4 cut(s) 282, 418, 789, 811
Mva1269I GAATGC 1 cut(s) 130
MvaI CCWGG 4 cut(s) 282, 418, 789, 811
MwoI GCNNNNNNNGC 4 cut(s) 35, 56, 331, 1127
NcoI CCATGG 1 cut(s) 171
NdeI CATATG 2 cut(s) 534, 1024
NlaIII CATG 7 cut(s) 175, 302, 350, 616, 740, 1130, 1168
NlaIV GGNNCC 3 cut(s) 415, 594, 607
NmuCI GTSAC 3 cut(s) 96, 754, 900
NspI RCATGY 1 cut(s) 1168
PagI TCATGA 1 cut(s) 612
PciI ACATGT 1 cut(s) 1164
PctI GAATGC 1 cut(s) 130
PfeI GAWTC 6 cut(s) 167, 193, 293, 653, 806, 1209
PflFI GACNNNGTC 1 cut(s) 898
PflMI CCANNNNNTGG 1 cut(s) 602
PfoI TCCNGGA 1 cut(s) 416
PkrI GCNGC 2 cut(s) 37, 106
Ple19I CGATCG 1 cut(s) 1141
PleI GAGTC 2 cut(s) 157, 1180
PpsI GAGTC 2 cut(s) 157, 1180
Ppu21I YACGTR 1 cut(s) 911
PscI ACATGT 1 cut(s) 1164
PsiI TTATAA 1 cut(s) 501
Psp6I CCWGG 4 cut(s) 280, 416, 787, 809
PspEI GGTNACC 1 cut(s) 96
PspGI CCWGG 4 cut(s) 280, 416, 787, 809
PspN4I GGNNCC 3 cut(s) 415, 594, 607
PstI CTGCAG 1 cut(s) 327
PstNI CAGNNNCTG 1 cut(s) 873
PsuI RGATCY 2 cut(s) 256, 592
PsyI GACNNNGTC 1 cut(s) 898
PvuI CGATCG 1 cut(s) 1141
RseI CAYNNNNRTG 1 cut(s) 1044
SalI GTCGAC 1 cut(s) 143
SaqAI TTAA 3 cut(s) 261, 921, 969
SatI GCNGC 2 cut(s) 36, 105
SchI GAGTC 2 cut(s) 158, 1181
ScrFI CCNGG 4 cut(s) 282, 418, 789, 811
SfaNI GCATC 3 cut(s) 313, 321, 1103
SfcI CTRYAG 2 cut(s) 323, 1131
SgrAI CRCCGGYG 1 cut(s) 100
SgrDI CGTCGACG 1 cut(s) 143
SmiMI CAYNNNNRTG 1 cut(s) 1044
SmlI CTYRAG 2 cut(s) 260, 560
SmoI CTYRAG 2 cut(s) 260, 560
Sse9I AATT 5 cut(s) 270, 368, 547, 1042, 1223
SsiI CCGC 5 cut(s) 76, 104, 107, 429, 706
StyD4I CCNGG 4 cut(s) 280, 416, 787, 809
StyI CCWWGG 1 cut(s) 171
TaaI ACNGT 3 cut(s) 142, 645, 763
TaiI ACGT 3 cut(s) 774, 907, 913
TaqI TCGA 4 cut(s) 144, 1141, 1161, 1204
TasI AATT 5 cut(s) 270, 368, 547, 1042, 1223
TauI GCSGC 1 cut(s) 107
TfiI GAWTC 6 cut(s) 167, 193, 293, 653, 806, 1209
Tru1I TTAA 3 cut(s) 261, 921, 969
Tru9I TTAA 3 cut(s) 261, 921, 969
TscAI CASTG 6 cut(s) 86, 558, 594, 631, 894, 953
TseFI GTSAC 3 cut(s) 96, 754, 900
TseI GCWGC 1 cut(s) 35
Tsp45I GTSAC 3 cut(s) 96, 754, 900
TspDTI ATGAA 5 cut(s) 17, 381, 629, 1083, 1115
TspGWI ACGGA 2 cut(s) 201, 1206
TspRI CASTG 6 cut(s) 86, 558, 594, 631, 894, 953
Tth111I GACNNNGTC 1 cut(s) 898
Van91I CCANNNNNTGG 1 cut(s) 602
Vha464I CTTAAG 1 cut(s) 260
XagI CCTNNNNNAGG 1 cut(s) 745
XapI RAATTY 2 cut(s) 368, 1223
XceI RCATGY 1 cut(s) 1168
XcmI CCANNNNNNNNNTGG 2 cut(s) 474, 894
XmiI GTMKAC 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.