Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0006721

Physical Location & Seq

Genomic Coordinates Reverse (-)

3637099 .. 3639341

Gene Structure 2243 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1043300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1179 bp

ATGGAGAAGGCTGGCTTCGGCAGCTTCCTTGAGAGCTCGAAACCCTACTTTGCAATGATCTCTCTACAATTCGGCTACGCCGGAATGAACATCATCACCAAAGTTTCTCTCAACAGAGGAATGAGTCACTATGTGCTTGTGGTTTATCGCCATGCCATTGCTACTGCTATTATAGCCCCATTTGCCTTCTTTTTTGAGAGGAAGAAGCAACCAAAAATGACATTCTCAATGTTCATGCAAATATTTATCCTCGGCCTTCTTGGTCCGGTGATTGACCAGAACTTCTACTATGCCGGGTTGAAGTTTACGTCTCCAACGTTCTCTTGTGCCATGAGCAACATGCTTCCGGCTATGACTTTTGTCCTGGCCGTCATATGCCGGATGGAAGTGCTAGACATCAAGAAAGTAAGGTGCCAAGCAAAGGTAATCGGAACAGTATTGACAGTGGCGGGGGCCATGCTGATGACCTTGTACAGGGGGCCAATTGTAGAGATGTTGTGGTCTAAGTATATTCACCCACGAAAATCATATGTCACAGATACCACTGGGACTGGTGACAAACACTGGTTTTTGGGATCCATTATGCTAACCATTGCTACTCTTGCCTGGGCTTCTTTATTTGTGTTACAAAACAACGCGTTGAAGACCTACAAGAACCATCAGCTGTCTCTCACATCAATGATATGCGGCATAGGAACTCTGCAGGCTATTGCTGTTACGTTTGTGATGGAACGCAAACCCTCCGTCTGGAGAATAGGTTTTGACATGAACCTCTTAGCTGCTGCCTATGCTGGAATTGTCACATCAAGCATTTCGTACTATGTGCAAGGGCTAGTGATGAAGATGAGAGGTCCCGTATTTGCAACTGCTTTTAGCCCTTTGATGATGATCATTGTGGCAATCATGGGATCCTTCATCCTTGCAGAAAAAATATTTCTTGGAAGCGTACTCGGAGCTGTATTGATCGTGTTCGGGCTTTATGCAGTCCTGTGGGGAAAACACAAAGAAGTATTGGAGAAGGAGGAGATCCCGGAAGTAATAAAGGGTTCTCAAGTAAACGGTGTGTCCATGTTCACCGTTAACGGAGATGTTGGAGCTAATACCAAGCTCTCCTCGGTTGCTATTTCAATGCCCATCACAGAACCCCCAATGAAAACCAACCCAATGCCAAATGCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005887 GO:0005938 GO:0006810 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009987 GO:0010033 GO:0016020 GO:0016021 GO:0022857 GO:0023052 GO:0031224 GO:0031226 GO:0032870 GO:0042221 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0065007 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071944 GO:0099568

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EamA

Protein Analysis

393

Amino Acids

43.22

Weight (kDa)

9.6

Isoelectric Point (pI)

29.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EamA	PF00892	16 - 156	2.6e-13	EamA-like transporter family
EamA	PF00892	191 - 330	1.5e-10	EamA-like transporter family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006721)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07050
fragaria_vesca	FvH4_1g29310 FvH4_1g29310 FvH4_7g04090 FvH4_7g04090
malus_domestica	MD02G1275400.v1.1 MD07G1043300.v1.1
prunus_persica	Prupe.2G045500_v2.0.a1 Prupe.2G045500_v2.0.a1
pyrus_communis	pycom02g23550 pycom07g03070
rosa_chinensis	RchiOBHm_Chr1g0327951 RchiOBHm_Chr3g0494821
rosa_laevigata	RLG00000022660 RLG00000029957
rosa_multiflora	Rmu_sc0000198.1_g000011 Rmu_ssc0000467.1_g000050
rosa_roxburghii	Rroxscaffold_4G00322120 Rroxscaffold_6G00390890
rosa_rugosa	Rorug01G0072500 Rorug03G0268300
rosa_samantha	Rh1BG071100 Rh1CG086600 Rh3DG351800
rosa_wichuraiana	Rw1G006880 Rw3G027680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	411
AccII	CGCG	1 cut(s)	638
AciI	CCGC	2 cut(s)	449, 687
AclI	AACGTT	1 cut(s)	317
AclWI	GGATC	5 cut(s)	570, 583, 903, 916, 1021
AcoI	YGGCCR	1 cut(s)	366
AdeI	CACNNNGTG	1 cut(s)	133
AfaI	GTAC	3 cut(s)	473, 818, 948
AfiI	CCNNNNNNNGG	3 cut(s)	421, 474, 747
AflIII	ACRYGT	1 cut(s)	636
AgsI	TTSAA	3 cut(s)	301, 643, 1128
AjnI	CCWGG	2 cut(s)	363, 605
AluBI	AGCT	7 cut(s)	24, 36, 664, 779, 956, 1097, 1108
AluI	AGCT	7 cut(s)	24, 36, 664, 779, 956, 1097, 1108
Alw21I	GWGCWC	1 cut(s)	38
Alw26I	GTCTC	2 cut(s)	315, 672
AlwI	GGATC	5 cut(s)	570, 583, 903, 916, 1021
AoxI	GGCC	4 cut(s)	253, 366, 453, 479
ApeKI	GCWGC	3 cut(s)	21, 779, 782
ArsI	GACNNNNNNTTYG	2 cut(s)	729, 761
AspS9I	GGNCC	4 cut(s)	263, 453, 479, 851
AsuC2I	CCSGG	2 cut(s)	295, 1031
AsuHPI	GGTGA	5 cut(s)	88, 280, 506, 566, 1066
AvaII	GGWCC	2 cut(s)	263, 851
BamHI	GGATCC	2 cut(s)	575, 908
BanI	GGYRCC	1 cut(s)	411
BanII	GRGCYC	1 cut(s)	38
BbsI	GAAGAC	1 cut(s)	650
Bbv12I	GWGCWC	1 cut(s)	38
BbvI	GCAGC	3 cut(s)	33, 766, 769
BccI	CCATC	4 cut(s)	376, 666, 721, 1142
BceAI	ACGGC	1 cut(s)	353
BciT130I	CCWGG	2 cut(s)	365, 607
BclI	TGATCA	1 cut(s)	888
BcnI	CCSGG	2 cut(s)	295, 1031
BcoDI	GTCTC	2 cut(s)	315, 672
BfaI	CTAG	3 cut(s)	392, 833, 1177
BfmI	CTRYAG	1 cut(s)	701
BisI	GCNGC	4 cut(s)	22, 688, 780, 783
BlsI	GCNGC	4 cut(s)	23, 689, 781, 784
Bme1390I	CCNGG	4 cut(s)	295, 365, 607, 1031
Bme18I	GGWCC	2 cut(s)	263, 851
BmgT120I	GGNCC	4 cut(s)	263, 453, 479, 851
BmiI	GGNNCC	6 cut(s)	413, 454, 480, 577, 853, 910
BmrFI	CCNGG	4 cut(s)	295, 365, 607, 1031
BmrI	ACTGGG	1 cut(s)	555
BmuI	ACTGGG	1 cut(s)	555
BoxI	GACNNNNGTC	1 cut(s)	359
BpiI	GAAGAC	1 cut(s)	650
BpmI	CTGGAG	1 cut(s)	769
BpuEI	CTTGAG	2 cut(s)	50, 1035
BpuMI	CCSGG	2 cut(s)	295, 1031
BsaBI	GATNNNNATC	1 cut(s)	887
BsaJI	CCNNGG	3 cut(s)	250, 606, 1113
BsaWI	WCCGGW	1 cut(s)	265
BsaXI	ACNNNNNCTCC	2 cut(s)	742, 772
Bsc4I	CCNNNNNNNGG	3 cut(s)	421, 474, 747
Bse1I	ACTGG	3 cut(s)	550, 556, 569
Bse3DI	GCAATG	3 cut(s)	60, 156, 591
Bse8I	GATNNNNATC	1 cut(s)	887
BseBI	CCWGG	2 cut(s)	365, 607
BseDI	CCNNGG	3 cut(s)	250, 606, 1113
BseGI	GGATG	2 cut(s)	387, 915
BseJI	GATNNNNATC	1 cut(s)	887
BseLI	CCNNNNNNNGG	3 cut(s)	421, 474, 747
BseMI	GCAATG	3 cut(s)	60, 156, 591
BseNI	ACTGG	3 cut(s)	550, 556, 569
BseRI	GAGGAG	2 cut(s)	1037, 1102
BseXI	GCAGC	3 cut(s)	33, 766, 769
Bsh1236I	CGCG	1 cut(s)	638
BshFI	GGCC	4 cut(s)	255, 368, 455, 481
BshNI	GGYRCC	1 cut(s)	411
BsiHKAI	GWGCWC	1 cut(s)	38
BsiSI	CCGG	6 cut(s)	81, 266, 294, 347, 379, 1031
BslFI	GGGAC	2 cut(s)	562, 837
BslI	CCNNNNNNNGG	3 cut(s)	421, 474, 747
BsmAI	GTCTC	2 cut(s)	315, 672
BsmBI	CGTCTC	1 cut(s)	315
BsmFI	GGGAC	2 cut(s)	562, 837
BsnI	GGCC	4 cut(s)	255, 368, 455, 481
Bsp1286I	GDGCHC	1 cut(s)	38
Bsp1407I	TGTACA	1 cut(s)	471
Bsp143I	GATC	6 cut(s)	57, 575, 888, 908, 963, 1026
BspACI	CCGC	2 cut(s)	449, 687
BspANI	GGCC	4 cut(s)	255, 368, 455, 481
BspFNI	CGCG	1 cut(s)	638
BspLI	GGNNCC	6 cut(s)	413, 454, 480, 577, 853, 910
BspMAI	CTGCAG	1 cut(s)	705
BspPI	GGATC	5 cut(s)	570, 583, 903, 916, 1021
BspT107I	GGYRCC	1 cut(s)	411
BsrDI	GCAATG	3 cut(s)	60, 156, 591
BsrGI	TGTACA	1 cut(s)	471
BsrI	ACTGG	3 cut(s)	550, 556, 569
BssECI	CCNNGG	3 cut(s)	250, 606, 1113
BssMI	GATC	6 cut(s)	57, 575, 888, 908, 963, 1026
Bst2UI	CCWGG	2 cut(s)	365, 607
Bst4CI	ACNGT	4 cut(s)	436, 445, 1061, 1078
BstAUI	TGTACA	1 cut(s)	471
BstC8I	GCNNGC	2 cut(s)	13, 705
BstDEI	CTNAG	2 cut(s)	504, 775
BstENI	CCTNNNNNAGG	1 cut(s)	472
BstF5I	GGATG	2 cut(s)	387, 915
BstFNI	CGCG	1 cut(s)	638
BstKTI	GATC	6 cut(s)	60, 578, 891, 911, 966, 1029
BstMAI	GTCTC	2 cut(s)	315, 672
BstMBI	GATC	6 cut(s)	57, 575, 888, 908, 963, 1026
BstMWI	GCNNNNNNNGC	5 cut(s)	21, 173, 182, 602, 788
BstNI	CCWGG	2 cut(s)	365, 607
BstNSI	RCATGY	1 cut(s)	343
BstPAI	GACNNNNGTC	1 cut(s)	359
BstSCI	CCNGG	4 cut(s)	293, 363, 605, 1029
BstSFI	CTRYAG	1 cut(s)	701
BstUI	CGCG	1 cut(s)	638
BstV1I	GCAGC	3 cut(s)	33, 766, 769
BstV2I	GAAGAC	1 cut(s)	650
BstX2I	RGATCY	3 cut(s)	575, 908, 1026
BstYI	RGATCY	3 cut(s)	575, 908, 1026
BsuRI	GGCC	4 cut(s)	255, 368, 455, 481
BtsCI	GGATG	2 cut(s)	387, 915
BtsIMutI	CAGTG	3 cut(s)	450, 543, 562
Cac8I	GCNNGC	2 cut(s)	13, 705
Cfr13I	GGNCC	4 cut(s)	263, 453, 479, 851
Csp6I	GTAC	3 cut(s)	472, 817, 947
CviAII	CATG	8 cut(s)	152, 235, 331, 340, 457, 766, 904, 1069
CviQI	GTAC	3 cut(s)	472, 817, 947
DdeI	CTNAG	2 cut(s)	504, 775
DpnI	GATC	6 cut(s)	59, 577, 890, 910, 965, 1028
DpnII	GATC	6 cut(s)	57, 575, 888, 908, 963, 1026
DraIII	CACNNNGTG	1 cut(s)	133
EaeI	YGGCCR	1 cut(s)	366
Ecl136II	GAGCTC	1 cut(s)	36
Eco24I	GRGCYC	1 cut(s)	38
Eco47I	GGWCC	2 cut(s)	263, 851
Eco53kI	GAGCTC	1 cut(s)	36
EcoICRI	GAGCTC	1 cut(s)	36
EcoNI	CCTNNNNNAGG	1 cut(s)	472
EcoO109I	RGGNCCY	1 cut(s)	851
EcoRII	CCWGG	2 cut(s)	363, 605
EcoT38I	GRGCYC	1 cut(s)	38
Esp3I	CGTCTC	1 cut(s)	315
FaeI	CATG	8 cut(s)	155, 238, 334, 343, 460, 769, 907, 1072
FalI	AAGNNNNNCTT	1 cut(s)	31
FaqI	GGGAC	2 cut(s)	562, 837
FatI	CATG	8 cut(s)	151, 234, 330, 339, 456, 765, 903, 1068
FauI	CCCGC	1 cut(s)	442
FauNDI	CATATG	2 cut(s)	374, 529
FbaI	TGATCA	1 cut(s)	888
Fnu4HI	GCNGC	4 cut(s)	22, 688, 780, 783
FokI	GGATG	2 cut(s)	394, 902
FriOI	GRGCYC	1 cut(s)	38
Fsp4HI	GCNGC	4 cut(s)	22, 688, 780, 783
FspBI	CTAG	3 cut(s)	392, 833, 1177
GluI	GCNGC	4 cut(s)	22, 688, 780, 783
GsuI	CTGGAG	1 cut(s)	769
HaeIII	GGCC	4 cut(s)	255, 368, 455, 481
HapII	CCGG	6 cut(s)	81, 266, 294, 347, 379, 1031
Hin1II	CATG	8 cut(s)	155, 238, 334, 343, 460, 769, 907, 1072
HincII	GTYRAC	1 cut(s)	1081
HindII	GTYRAC	1 cut(s)	1081
HinfI	GANTC	1 cut(s)	124
HpaI	GTTAAC	1 cut(s)	1081
HpaII	CCGG	6 cut(s)	81, 266, 294, 347, 379, 1031
HphI	GGTGA	5 cut(s)	88, 280, 506, 566, 1066
Hpy166II	GTNNAC	4 cut(s)	306, 1057, 1074, 1081
Hpy188I	TCNGA	2 cut(s)	431, 953
Hpy188III	TCNNGA	2 cut(s)	400, 748
Hpy8I	GTNNAC	4 cut(s)	306, 1057, 1074, 1081
HpyAV	CCTTC	4 cut(s)	196, 266, 922, 1012
HpyCH4III	ACNGT	4 cut(s)	436, 445, 1061, 1078
HpyCH4IV	ACGT	3 cut(s)	308, 317, 719
HpyCH4V	TGCA	7 cut(s)	53, 238, 703, 826, 863, 923, 983
HpyF10VI	GCNNNNNNNGC	5 cut(s)	21, 173, 182, 602, 788
HpyF3I	CTNAG	2 cut(s)	504, 775
HpySE526I	ACGT	3 cut(s)	308, 317, 719
Hsp92II	CATG	8 cut(s)	155, 238, 334, 343, 460, 769, 907, 1072
Ksp22I	TGATCA	1 cut(s)	888
KspAI	GTTAAC	1 cut(s)	1081
Kzo9I	GATC	6 cut(s)	57, 575, 888, 908, 963, 1026
LmnI	GCTCC	2 cut(s)	953, 1094
Lsp1109I	GCAGC	3 cut(s)	33, 766, 769
MaeI	CTAG	3 cut(s)	392, 833, 1177
MaeII	ACGT	3 cut(s)	308, 317, 719
MaeIII	GTNAC	6 cut(s)	125, 532, 554, 624, 715, 799
MalI	GATC	6 cut(s)	59, 577, 890, 910, 965, 1028
MboI	GATC	6 cut(s)	57, 575, 888, 908, 963, 1026
MboII	GAAGA	3 cut(s)	214, 655, 853
MfeI	CAATTG	1 cut(s)	483
MflI	RGATCY	3 cut(s)	575, 908, 1026
MhlI	GDGCHC	1 cut(s)	38
MluCI	AATT	3 cut(s)	68, 483, 795
MluI	ACGCGT	1 cut(s)	636
MlyI	GAGTC	1 cut(s)	133
MmeI	TCCRAC	2 cut(s)	338, 1072
MnlI	CCTC	8 cut(s)	110, 192, 260, 751, 782, 842, 1015, 1123
MseI	TTAA	1 cut(s)	1080
MslI	CAYNNNNRTG	2 cut(s)	461, 677
MspA1I	CMGCKG	1 cut(s)	664
MspI	CCGG	6 cut(s)	81, 266, 294, 347, 379, 1031
MspR9I	CCNGG	4 cut(s)	295, 365, 607, 1031
MunI	CAATTG	1 cut(s)	483
MvaI	CCWGG	2 cut(s)	365, 607
MvnI	CGCG	1 cut(s)	638
MwoI	GCNNNNNNNGC	5 cut(s)	21, 173, 182, 602, 788
NciI	CCSGG	2 cut(s)	295, 1031
NdeI	CATATG	2 cut(s)	374, 529
NdeII	GATC	6 cut(s)	57, 575, 888, 908, 963, 1026
NlaIII	CATG	8 cut(s)	155, 238, 334, 343, 460, 769, 907, 1072
NlaIV	GGNNCC	6 cut(s)	413, 454, 480, 577, 853, 910
NmeAIII	GCCGAG	1 cut(s)	231
NmuCI	GTSAC	4 cut(s)	125, 532, 554, 799
NspI	RCATGY	1 cut(s)	343
PcsI	WCGNNNNNNNCGW	1 cut(s)	314
PfoI	TCCNGGA	1 cut(s)	1029
PkrI	GCNGC	4 cut(s)	23, 689, 781, 784
PleI	GAGTC	1 cut(s)	132
PpsI	GAGTC	1 cut(s)	132
PpuMI	RGGWCCY	1 cut(s)	851
PshAI	GACNNNNGTC	1 cut(s)	359
Psp124BI	GAGCTC	1 cut(s)	38
Psp1406I	AACGTT	1 cut(s)	317
Psp5II	RGGWCCY	1 cut(s)	851
Psp6I	CCWGG	2 cut(s)	363, 605
PspGI	CCWGG	2 cut(s)	363, 605
PspN4I	GGNNCC	6 cut(s)	413, 454, 480, 577, 853, 910
PspPI	GGNCC	4 cut(s)	263, 453, 479, 851
PspPPI	RGGWCCY	1 cut(s)	851
PstI	CTGCAG	1 cut(s)	705
PsuI	RGATCY	3 cut(s)	575, 908, 1026
PvuII	CAGCTG	1 cut(s)	664
RsaI	GTAC	3 cut(s)	473, 818, 948
RsaNI	GTAC	3 cut(s)	472, 817, 947
RseI	CAYNNNNRTG	2 cut(s)	461, 677
SacI	GAGCTC	1 cut(s)	38
SaqAI	TTAA	1 cut(s)	1080
SatI	GCNGC	4 cut(s)	22, 688, 780, 783
Sau3AI	GATC	6 cut(s)	57, 575, 888, 908, 963, 1026
Sau96I	GGNCC	4 cut(s)	263, 453, 479, 851
SchI	GAGTC	1 cut(s)	133
ScrFI	CCNGG	4 cut(s)	295, 365, 607, 1031
SduI	GDGCHC	1 cut(s)	38
SfcI	CTRYAG	1 cut(s)	701
SinI	GGWCC	2 cut(s)	263, 851
SmiMI	CAYNNNNRTG	2 cut(s)	461, 677
SmlI	CTYRAG	2 cut(s)	29, 1050
SmoI	CTYRAG	2 cut(s)	29, 1050
Sse9I	AATT	3 cut(s)	68, 483, 795
SsiI	CCGC	2 cut(s)	449, 687
SspI	AATATT	2 cut(s)	243, 933
SspMI	CTAG	3 cut(s)	392, 833, 1177
SstI	GAGCTC	1 cut(s)	38
StyD4I	CCNGG	4 cut(s)	293, 363, 605, 1029
TaaI	ACNGT	4 cut(s)	436, 445, 1061, 1078
TaiI	ACGT	3 cut(s)	311, 320, 722
TaqI	TCGA	1 cut(s)	38
TasI	AATT	3 cut(s)	68, 483, 795
TatI	WGTACW	1 cut(s)	471
TauI	GCSGC	1 cut(s)	690
Tru1I	TTAA	1 cut(s)	1080
Tru9I	TTAA	1 cut(s)	1080
TscAI	CASTG	3 cut(s)	450, 550, 569
TseFI	GTSAC	4 cut(s)	125, 532, 554, 799
TseI	GCWGC	3 cut(s)	21, 779, 782
Tsp45I	GTSAC	4 cut(s)	125, 532, 554, 799
TspDTI	ATGAA	6 cut(s)	101, 223, 782, 854, 904, 1166
TspGWI	ACGGA	2 cut(s)	733, 1098
TspRI	CASTG	3 cut(s)	450, 550, 569
VpaK11BI	GGWCC	2 cut(s)	263, 851
XagI	CCTNNNNNAGG	1 cut(s)	472
XceI	RCATGY	1 cut(s)	343
XspI	CTAG	3 cut(s)	392, 833, 1177

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.