MD07G1067500.v1.1

Transcriptional activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0009830
Physical Location & Seq
Forward (+)
6341109 .. 6342330
1222 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1067500.v1.1.491

Sequence Viewer

Length: 489 bp
ATGCAGAAAATGAAGAGCCTTGGGAGTAATTCCTTTGCCGGTAGTAGTGGGAGGCTGTCGACGGAGGAGAACGACGATGAGGAGATTTCGAGGCTGGCAATATCGGCGTACCAAGCTAGAGAGGAAGAGATTGAGAGGAAGAAGATGGAGGTTAAGGAGAAAGTTGAAGCTCAGTTGGGTCGCGCTGAGGAAGAAACTAGGCGTCTGGCGCAGATTTGGGAAGAGCTGGAAGTGCTGGGAGATCCCATGAGGAAGGAACTTGCAAATGTAAGGAAGAAAATCGACATGGCTAATCGAGAGCTGAAGCCGCTGGGATTGAGCTGCCAGAAGAAGGAAAAAGAATACAAAGAAGCCCTGGAAAGCTTCAATGAAAAGAACAAAGAAAAGGCTCAACTTGTAAGCACACTTATGGAGCTGTTGACCGAGAGTGAGAAGCTGAGGATGCAGAAGCTAGAGGAGCTGAGCAAGAACATAGAACCAACACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04115 map04115
Pfam Domains
Protein Families
Transcrip_act

Protein Analysis

163

Amino Acids

18.78

Weight (kDa)

5.31

Isoelectric Point (pI)

53.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transcrip_act PF04949 13 - 159 7e-74 Transcriptional activator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 59
AccII CGCG 1 cut(s) 183
AciI CCGC 1 cut(s) 308
AclWI GGATC 1 cut(s) 236
AcuI CTGAAG 1 cut(s) 323
AcyI GRCGYC 1 cut(s) 202
AfaI GTAC 1 cut(s) 110
AfiI CCNNNNNNNGG 1 cut(s) 331
AgsI TTSAA 2 cut(s) 167, 367
AjnI CCWGG 1 cut(s) 354
AlwI GGATC 1 cut(s) 236
ApeKI GCWGC 1 cut(s) 321
AspLEI GCGC 2 cut(s) 185, 211
BbvCI CCTCAGC 2 cut(s) 186, 437
BbvI GCAGC 1 cut(s) 308
BccI CCATC 1 cut(s) 139
BciT130I CCWGG 1 cut(s) 356
BfaI CTAG 3 cut(s) 117, 198, 452
BisI GCNGC 2 cut(s) 308, 322
BlpI GCTNAGC 1 cut(s) 461
BlsI GCNGC 2 cut(s) 309, 323
Bme1390I CCNGG 1 cut(s) 356
BmrFI CCNGG 1 cut(s) 356
BmsI GCATC 1 cut(s) 432
Bpu10I CCTNAGC 2 cut(s) 186, 437
Bpu1102I GCTNAGC 1 cut(s) 461
BsaHI GRCGYC 1 cut(s) 202
BsaJI CCNNGG 2 cut(s) 19, 354
Bsc4I CCNNNNNNNGG 1 cut(s) 331
Bse118I RCCGGY 1 cut(s) 38
BseBI CCWGG 1 cut(s) 356
BseDI CCNNGG 2 cut(s) 19, 354
BseGI GGATG 1 cut(s) 447
BseLI CCNNNNNNNGG 1 cut(s) 331
BseMII CTCAG 4 cut(s) 177, 185, 428, 452
BseRI GAGGAG 3 cut(s) 80, 95, 470
BseXI GCAGC 1 cut(s) 308
BseYI CCCAGC 2 cut(s) 235, 310
Bsh1236I CGCG 1 cut(s) 183
BsiSI CCGG 1 cut(s) 39
BslI CCNNNNNNNGG 1 cut(s) 331
Bsp143I GATC 1 cut(s) 241
Bsp1720I GCTNAGC 1 cut(s) 461
BspACI CCGC 1 cut(s) 308
BspCNI CTCAG 4 cut(s) 178, 184, 429, 453
BspFNI CGCG 1 cut(s) 183
BspPI GGATC 1 cut(s) 236
BspQI GCTCTTC 2 cut(s) 8, 216
BsrFI RCCGGY 1 cut(s) 38
BssAI RCCGGY 1 cut(s) 38
BssECI CCNNGG 2 cut(s) 19, 354
BssMI GATC 1 cut(s) 241
BssNI GRCGYC 1 cut(s) 202
BssT1I CCWWGG 1 cut(s) 19
Bst2UI CCWGG 1 cut(s) 356
Bst6I CTCTTC 3 cut(s) 8, 120, 216
BstACI GRCGYC 1 cut(s) 202
BstC8I GCNNGC 1 cut(s) 96
BstDEI CTNAG 4 cut(s) 171, 186, 437, 461
BstF5I GGATG 1 cut(s) 447
BstFNI CGCG 1 cut(s) 183
BstHHI GCGC 2 cut(s) 185, 211
BstKTI GATC 1 cut(s) 244
BstMBI GATC 1 cut(s) 241
BstMWI GCNNNNNNNGC 7 cut(s) 104, 113, 208, 232, 307, 442, 457
BstNI CCWGG 1 cut(s) 356
BstSCI CCNGG 1 cut(s) 354
BstUI CGCG 1 cut(s) 183
BstV1I GCAGC 1 cut(s) 308
BstX2I RGATCY 1 cut(s) 241
BstYI RGATCY 1 cut(s) 241
BtsCI GGATG 1 cut(s) 447
Cac8I GCNNGC 1 cut(s) 96
CfoI GCGC 2 cut(s) 185, 211
Cfr10I RCCGGY 1 cut(s) 38
CseI GACGC 1 cut(s) 191
Csp6I GTAC 1 cut(s) 109
CviAII CATG 2 cut(s) 247, 286
CviQI GTAC 1 cut(s) 109
DdeI CTNAG 4 cut(s) 171, 186, 437, 461
DpnI GATC 1 cut(s) 243
DpnII GATC 1 cut(s) 241
Eam1104I CTCTTC 3 cut(s) 8, 120, 216
EarI CTCTTC 3 cut(s) 8, 120, 216
Eco130I CCWWGG 1 cut(s) 19
Eco57I CTGAAG 1 cut(s) 323
EcoRII CCWGG 1 cut(s) 354
EcoT14I CCWWGG 1 cut(s) 19
ErhI CCWWGG 1 cut(s) 19
FaeI CATG 2 cut(s) 250, 289
FaiI YATR 5 cut(s) 248, 287, 410, 473, 487
FatI CATG 2 cut(s) 246, 285
FblI GTMKAC 1 cut(s) 59
Fnu4HI GCNGC 2 cut(s) 308, 322
FokI GGATG 1 cut(s) 454
Fsp4HI GCNGC 2 cut(s) 308, 322
FspBI CTAG 3 cut(s) 117, 198, 452
GlaI GCGC 2 cut(s) 184, 210
GluI GCNGC 2 cut(s) 308, 322
GsaI CCCAGC 2 cut(s) 239, 314
HapII CCGG 1 cut(s) 39
HgaI GACGC 1 cut(s) 191
HhaI GCGC 2 cut(s) 185, 211
Hin1I GRCGYC 1 cut(s) 202
Hin1II CATG 2 cut(s) 250, 289
Hin6I GCGC 2 cut(s) 183, 209
HinP1I GCGC 2 cut(s) 183, 209
HincII GTYRAC 2 cut(s) 60, 420
HindII GTYRAC 2 cut(s) 60, 420
HindIII AAGCTT 1 cut(s) 361
HpaII CCGG 1 cut(s) 39
Hpy166II GTNNAC 2 cut(s) 60, 420
Hpy188III TCNNGA 1 cut(s) 296
Hpy8I GTNNAC 2 cut(s) 60, 420
Hpy99I CGWCG 2 cut(s) 64, 77
HpyAV CCTTC 2 cut(s) 247, 325
HpyCH4V TGCA 3 cut(s) 4, 263, 445
HpyF10VI GCNNNNNNNGC 7 cut(s) 104, 113, 208, 232, 307, 442, 457
HpyF3I CTNAG 4 cut(s) 171, 186, 437, 461
Hsp92I GRCGYC 1 cut(s) 202
Hsp92II CATG 2 cut(s) 250, 289
HspAI GCGC 2 cut(s) 183, 209
Kzo9I GATC 1 cut(s) 241
LguI GCTCTTC 2 cut(s) 8, 216
LmnI GCTCC 2 cut(s) 412, 457
LpnPI CCDG 9 cut(s) 52, 80, 191, 212, 221, 296, 338, 341, 368
Lsp1109I GCAGC 1 cut(s) 308
LweI GCATC 1 cut(s) 432
MaeI CTAG 3 cut(s) 117, 198, 452
MalI GATC 1 cut(s) 243
MboI GATC 1 cut(s) 241
MboII GAAGA 8 cut(s) 25, 137, 151, 154, 203, 233, 286, 340
MflI RGATCY 1 cut(s) 241
MluCI AATT 1 cut(s) 28
MseI TTAA 1 cut(s) 153
MslI CAYNNNNRTG 1 cut(s) 407
MspA1I CMGCKG 1 cut(s) 310
MspI CCGG 1 cut(s) 39
MspR9I CCNGG 1 cut(s) 356
MvaI CCWGG 1 cut(s) 356
MvnI CGCG 1 cut(s) 183
MwoI GCNNNNNNNGC 7 cut(s) 104, 113, 208, 232, 307, 442, 457
NdeII GATC 1 cut(s) 241
NlaIII CATG 2 cut(s) 250, 289
PciSI GCTCTTC 2 cut(s) 8, 216
PkrI GCNGC 2 cut(s) 309, 323
Psp6I CCWGG 1 cut(s) 354
PspFI CCCAGC 2 cut(s) 235, 310
PspGI CCWGG 1 cut(s) 354
PsuI RGATCY 1 cut(s) 241
RsaI GTAC 1 cut(s) 110
RsaNI GTAC 1 cut(s) 109
RseI CAYNNNNRTG 1 cut(s) 407
SalI GTCGAC 1 cut(s) 58
SapI GCTCTTC 2 cut(s) 8, 216
SaqAI TTAA 1 cut(s) 153
SatI GCNGC 2 cut(s) 308, 322
Sau3AI GATC 1 cut(s) 241
ScrFI CCNGG 1 cut(s) 356
SfaNI GCATC 1 cut(s) 432
SmiMI CAYNNNNRTG 1 cut(s) 407
Sse9I AATT 1 cut(s) 28
SsiI CCGC 1 cut(s) 308
SspMI CTAG 3 cut(s) 117, 198, 452
StyD4I CCNGG 1 cut(s) 354
StyI CCWWGG 1 cut(s) 19
TaqI TCGA 4 cut(s) 59, 89, 282, 295
TaqII GACCGA 1 cut(s) 437
TasI AATT 1 cut(s) 28
TauI GCSGC 1 cut(s) 310
Tru1I TTAA 1 cut(s) 153
Tru9I TTAA 1 cut(s) 153
TseI GCWGC 1 cut(s) 321
TspDTI ATGAA 2 cut(s) 26, 384
TspGWI ACGGA 1 cut(s) 77
XmiI GTMKAC 1 cut(s) 59
XspI CTAG 3 cut(s) 117, 198, 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.