MD07G1068400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0008763
Physical Location & Seq
Forward (+)
6472215 .. 6472721
507 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1068400.v1.1.491

Sequence Viewer

Length: 507 bp
ATGGAGAGGCCGGAAGGATATCCACAAGTAGATGATATTGCTACATATACAGAGCCATCGAGCACGAGCACTTCATTTTCGGGCACCAAGAATGATATGTCACAGCTCGAAAGCGGCCTACTTCGAAGAAATGTTAGCGACTTAGGCGACAGTTACTACAGATTCCAATCCCAAGTTGGAAAGTCTGTTGATCCAAAGTTGTTGGCGTTGGTAGAGTTTTTCAGGGAACTTTATTTTCGAAGGCTGGAACTGTTCAAGAAAATATTTCCGGGGATTCAAGATAAGTTTCTTGAGGTGCCAAAGAAATTGCATGTGATATTAGCTCAAGTGAAACCGGATGAAACCAAACTAGCTCGAACTATGCAACGGAGTCTGAGCGTTGGTTCACCGCGGGATTTCAAAGGAGGGGAATCGAAACTTAGGCTCGAGCGATTCAAGGTGAGGACTATTGATACAGGCGATGGTGTTGAACAAGGTGGCCTGCAGGGTAGTAAACCGGCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

169

Amino Acids

19.01

Weight (kDa)

9.39

Isoelectric Point (pI)

36.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 83, 295
AccII CGCG 1 cut(s) 391
AciI CCGC 3 cut(s) 114, 389, 391
AclWI GGATC 1 cut(s) 185
AcoI YGGCCR 1 cut(s) 498
AgsI TTSAA 5 cut(s) 256, 278, 400, 436, 470
AluBI AGCT 3 cut(s) 106, 323, 353
AluI AGCT 3 cut(s) 106, 323, 353
Alw21I GWGCWC 2 cut(s) 65, 71
AlwI GGATC 1 cut(s) 185
Ama87I CYCGRG 1 cut(s) 425
AoxI GGCC 4 cut(s) 8, 115, 478, 498
AsuC2I CCSGG 1 cut(s) 270
AsuHPI GGTGA 2 cut(s) 378, 451
AsuII TTCGAA 2 cut(s) 124, 238
AvaI CYCGRG 1 cut(s) 425
BaeGI GKGCMC 1 cut(s) 86
BanI GGYRCC 2 cut(s) 83, 295
BauI CACGAG 1 cut(s) 64
Bbv12I GWGCWC 2 cut(s) 65, 71
BccI CCATC 2 cut(s) 64, 455
BcnI CCSGG 1 cut(s) 270
BfaI CTAG 1 cut(s) 350
BfmI CTRYAG 2 cut(s) 157, 482
BisI GCNGC 1 cut(s) 115
BlsI GCNGC 1 cut(s) 116
Bme1390I CCNGG 1 cut(s) 270
BmeT110I CYCGRG 1 cut(s) 425
BmiI GGNNCC 2 cut(s) 85, 297
BmrFI CCNGG 1 cut(s) 270
Bpu14I TTCGAA 2 cut(s) 124, 238
BpuEI CTTGAG 2 cut(s) 309, 311
BpuMI CCSGG 1 cut(s) 270
BsaBI GATNNNNATC 1 cut(s) 166
BsaJI CCNNGG 2 cut(s) 269, 389
BsaWI WCCGGW 1 cut(s) 334
Bse118I RCCGGY 1 cut(s) 496
Bse8I GATNNNNATC 1 cut(s) 166
BseDI CCNNGG 2 cut(s) 269, 389
BseGI GGATG 1 cut(s) 343
BseJI GATNNNNATC 1 cut(s) 166
BseMII CTCAG 1 cut(s) 365
BseSI GKGCMC 1 cut(s) 86
Bsh1236I CGCG 1 cut(s) 391
BshFI GGCC 4 cut(s) 10, 117, 480, 500
BshNI GGYRCC 2 cut(s) 83, 295
BsiHKAI GWGCWC 2 cut(s) 65, 71
BsiHKCI CYCGRG 1 cut(s) 425
BsiSI CCGG 4 cut(s) 11, 269, 335, 497
BsnI GGCC 4 cut(s) 10, 117, 480, 500
BsoBI CYCGRG 1 cut(s) 425
Bsp119I TTCGAA 2 cut(s) 124, 238
Bsp1286I GDGCHC 3 cut(s) 65, 71, 86
Bsp143I GATC 1 cut(s) 190
BspACI CCGC 3 cut(s) 114, 389, 391
BspANI GGCC 4 cut(s) 10, 117, 480, 500
BspCNI CTCAG 1 cut(s) 366
BspFNI CGCG 1 cut(s) 391
BspLI GGNNCC 2 cut(s) 85, 297
BspMAI CTGCAG 1 cut(s) 486
BspPI GGATC 1 cut(s) 185
BspT104I TTCGAA 2 cut(s) 124, 238
BspT107I GGYRCC 2 cut(s) 83, 295
BsrFI RCCGGY 1 cut(s) 496
BssAI RCCGGY 1 cut(s) 496
BssECI CCNNGG 2 cut(s) 269, 389
BssMI GATC 1 cut(s) 190
BssSI CACGAG 1 cut(s) 64
Bst2BI CACGAG 1 cut(s) 64
Bst4CI ACNGT 2 cut(s) 152, 252
BstBI TTCGAA 2 cut(s) 124, 238
BstC8I GCNNGC 1 cut(s) 482
BstDEI CTNAG 3 cut(s) 142, 374, 419
BstDSI CCRYGG 1 cut(s) 389
BstF5I GGATG 1 cut(s) 343
BstFNI CGCG 1 cut(s) 391
BstKTI GATC 1 cut(s) 193
BstMBI GATC 1 cut(s) 190
BstMWI GCNNNNNNNGC 1 cut(s) 144
BstNSI RCATGY 1 cut(s) 314
BstSCI CCNGG 1 cut(s) 268
BstSFI CTRYAG 2 cut(s) 157, 482
BstSLI GKGCMC 1 cut(s) 86
BstUI CGCG 1 cut(s) 391
BsuRI GGCC 4 cut(s) 10, 117, 480, 500
BtgI CCRYGG 1 cut(s) 389
BtgZI GCGATG 1 cut(s) 474
BtsCI GGATG 1 cut(s) 343
Cac8I GCNNGC 1 cut(s) 482
Cfr10I RCCGGY 1 cut(s) 496
Cfr42I CCGCGG 1 cut(s) 392
CviAII CATG 1 cut(s) 311
DdeI CTNAG 3 cut(s) 142, 374, 419
DpnI GATC 1 cut(s) 192
DpnII GATC 1 cut(s) 190
EaeI YGGCCR 1 cut(s) 498
Eco32I GATATC 1 cut(s) 20
Eco88I CYCGRG 1 cut(s) 425
EcoRV GATATC 1 cut(s) 20
FaeI CATG 1 cut(s) 314
FaiI YATR 5 cut(s) 46, 48, 98, 312, 362
FatI CATG 1 cut(s) 310
FauI CCCGC 1 cut(s) 384
Fnu4HI GCNGC 1 cut(s) 115
FokI GGATG 1 cut(s) 350
Fsp4HI GCNGC 1 cut(s) 115
FspBI CTAG 1 cut(s) 350
GluI GCNGC 1 cut(s) 115
HaeIII GGCC 4 cut(s) 10, 117, 480, 500
HapII CCGG 4 cut(s) 11, 269, 335, 497
Hin1II CATG 1 cut(s) 314
HinfI GANTC 5 cut(s) 162, 274, 370, 410, 432
HpaII CCGG 4 cut(s) 11, 269, 335, 497
HphI GGTGA 2 cut(s) 378, 451
Hpy166II GTNNAC 2 cut(s) 386, 494
Hpy188I TCNGA 1 cut(s) 375
Hpy188III TCNNGA 3 cut(s) 256, 278, 290
Hpy8I GTNNAC 2 cut(s) 386, 494
HpyAV CCTTC 2 cut(s) 8, 234
HpyCH4III ACNGT 2 cut(s) 152, 252
HpyCH4V TGCA 3 cut(s) 310, 364, 484
HpyF10VI GCNNNNNNNGC 1 cut(s) 144
HpyF3I CTNAG 3 cut(s) 142, 374, 419
Hsp92II CATG 1 cut(s) 314
KspI CCGCGG 1 cut(s) 392
Kzo9I GATC 1 cut(s) 190
LpnPI CCDG 8 cut(s) 24, 208, 230, 282, 348, 441, 470, 494
MaeI CTAG 1 cut(s) 350
MaeIII GTNAC 2 cut(s) 99, 152
MalI GATC 1 cut(s) 192
MboI GATC 1 cut(s) 190
MboII GAAGA 1 cut(s) 138
MhlI GDGCHC 3 cut(s) 65, 71, 86
MluCI AATT 1 cut(s) 305
MlyI GAGTC 1 cut(s) 379
MmeI TCCRAC 1 cut(s) 157
MnlI CCTC 3 cut(s) 286, 398, 435
MspA1I CMGCKG 1 cut(s) 391
MspI CCGG 4 cut(s) 11, 269, 335, 497
MspR9I CCNGG 1 cut(s) 270
MvnI CGCG 1 cut(s) 391
MwoI GCNNNNNNNGC 1 cut(s) 144
NciI CCSGG 1 cut(s) 270
NdeII GATC 1 cut(s) 190
NlaIII CATG 1 cut(s) 314
NlaIV GGNNCC 2 cut(s) 85, 297
NmuCI GTSAC 1 cut(s) 99
NspI RCATGY 1 cut(s) 314
NspV TTCGAA 2 cut(s) 124, 238
PaeR7I CTCGAG 1 cut(s) 425
PfeI GAWTC 4 cut(s) 162, 274, 410, 432
PkrI GCNGC 1 cut(s) 116
PleI GAGTC 1 cut(s) 378
PpsI GAGTC 1 cut(s) 378
PspN4I GGNNCC 2 cut(s) 85, 297
PspXI VCTCGAGB 1 cut(s) 425
PstI CTGCAG 1 cut(s) 486
SacII CCGCGG 1 cut(s) 392
SatI GCNGC 1 cut(s) 115
Sau3AI GATC 1 cut(s) 190
SbfI CCTGCAGG 1 cut(s) 486
SchI GAGTC 1 cut(s) 379
ScrFI CCNGG 1 cut(s) 270
SdaI CCTGCAGG 1 cut(s) 486
SduI GDGCHC 3 cut(s) 65, 71, 86
SetI ASST 6 cut(s) 108, 297, 325, 355, 441, 478
SfcI CTRYAG 2 cut(s) 157, 482
Sfr274I CTCGAG 1 cut(s) 425
Sfr303I CCGCGG 1 cut(s) 392
SfuI TTCGAA 2 cut(s) 124, 238
SgrBI CCGCGG 1 cut(s) 392
SlaI CTCGAG 1 cut(s) 425
SmlI CTYRAG 3 cut(s) 290, 324, 425
SmoI CTYRAG 3 cut(s) 290, 324, 425
Sse8387I CCTGCAGG 1 cut(s) 486
Sse9I AATT 1 cut(s) 305
SsiI CCGC 3 cut(s) 114, 389, 391
SspI AATATT 1 cut(s) 264
SspMI CTAG 1 cut(s) 350
StyD4I CCNGG 1 cut(s) 268
TaaI ACNGT 2 cut(s) 152, 252
TaqI TCGA 7 cut(s) 59, 108, 124, 238, 355, 413, 426
TasI AATT 1 cut(s) 305
TauI GCSGC 1 cut(s) 117
TfiI GAWTC 4 cut(s) 162, 274, 410, 432
TseFI GTSAC 1 cut(s) 99
Tsp45I GTSAC 1 cut(s) 99
TspDTI ATGAA 2 cut(s) 63, 354
TspGWI ACGGA 1 cut(s) 382
XceI RCATGY 1 cut(s) 314
XcmI CCANNNNNNNNNTGG 1 cut(s) 173
XhoI CTCGAG 1 cut(s) 425
XspI CTAG 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.