pycom02g21230

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0008763
Physical Location & Seq
Reverse (-)
19398741 .. 19399263
523 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g21230.2

Sequence Viewer

Length: 477 bp
ATGGAGAAGCCAGAAGGATATCCACAAGTAGACGATATTGCAACAAGTACCGAGCCATCAAGCTCAGGAACTTCATTCTTCGGCGCCAAGAACGATATGTCACTGCTCGAGAGCGGCCTACTCCGGAGAAGTGTTAGTGATATAGGTGAAACTTACTACAGATTCCAGTCAAAAATTGGCAACTCAGTTGATCCAAGGTTGCTAGCGCTGGTGGAATTCTTTAGAGAACTTTATTTTCGAAGATTTGAACTGTTCAAGAAAATATTTCCCGGGGTTCAGGATAAGTTAATTATTGACGTGCACCCACTTCCTGATCAAACCAAAGCAGTTCGGACAATGCAACGGAGTCTAAGTATTGGTTCTCCGCGTGATTTCAAAGGAAGAGAATCCGAAATGAGGCTTGAGAGATTCAAGGTAAGGACCATCGATGTAGATGATGGCGTTGATCAAACTGCTAAACCCAGTGGCTCGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

159

Amino Acids

17.98

Weight (kDa)

7.92

Isoelectric Point (pI)

32.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 83
AccBSI CCGCTC 1 cut(s) 114
AccI GTMKAC 1 cut(s) 30
AccII CGCG 1 cut(s) 367
AccIII TCCGGA 1 cut(s) 123
AciI CCGC 2 cut(s) 114, 365
AclWI GGATC 1 cut(s) 185
AcsI RAATTY 1 cut(s) 215
AcyI GRCGYC 1 cut(s) 84
AfaI GTAC 1 cut(s) 49
AfeI AGCGCT 1 cut(s) 207
AfiI CCNNNNNNNGG 1 cut(s) 396
AgsI TTSAA 4 cut(s) 248, 256, 376, 412
AjiI CACGTC 1 cut(s) 298
AluBI AGCT 1 cut(s) 63
AluI AGCT 1 cut(s) 63
Alw21I GWGCWC 1 cut(s) 303
Alw44I GTGCAC 1 cut(s) 299
AlwI GGATC 1 cut(s) 185
Ama87I CYCGRG 2 cut(s) 107, 269
Aor13HI TCCGGA 1 cut(s) 123
Aor51HI AGCGCT 1 cut(s) 207
AoxI GGCC 1 cut(s) 115
ApaLI GTGCAC 1 cut(s) 299
ApoI RAATTY 1 cut(s) 215
AspLEI GCGC 2 cut(s) 86, 208
AspS9I GGNCC 1 cut(s) 420
AsuC2I CCSGG 2 cut(s) 270, 271
AsuHPI GGTGA 1 cut(s) 158
AsuII TTCGAA 1 cut(s) 238
AsuNHI GCTAGC 1 cut(s) 202
AvaI CYCGRG 2 cut(s) 107, 269
AvaII GGWCC 1 cut(s) 420
BaeGI GKGCMC 1 cut(s) 303
BanI GGYRCC 1 cut(s) 83
Bbv12I GWGCWC 1 cut(s) 303
BccI CCATC 3 cut(s) 64, 431, 431
BclI TGATCA 2 cut(s) 313, 445
BcnI CCSGG 2 cut(s) 270, 271
BfaI CTAG 1 cut(s) 203
BfmI CTRYAG 1 cut(s) 157
BfoI RGCGCY 2 cut(s) 87, 209
BisI GCNGC 1 cut(s) 115
BlsI GCNGC 1 cut(s) 116
Bme1390I CCNGG 2 cut(s) 270, 271
Bme18I GGWCC 1 cut(s) 420
BmeT110I CYCGRG 2 cut(s) 107, 269
BmgBI CACGTC 1 cut(s) 298
BmgT120I GGNCC 1 cut(s) 420
BmiI GGNNCC 1 cut(s) 85
BmrFI CCNGG 2 cut(s) 270, 271
BmrI ACTGGG 1 cut(s) 456
BmtI GCTAGC 1 cut(s) 206
BmuI ACTGGG 1 cut(s) 456
Bpu10I CCTNAGC 1 cut(s) 64
Bpu14I TTCGAA 1 cut(s) 238
BpuEI CTTGAG 1 cut(s) 422
BpuMI CCSGG 2 cut(s) 270, 271
Bsa29I ATCGAT 1 cut(s) 426
BsaHI GRCGYC 1 cut(s) 84
BsaJI CCNNGG 3 cut(s) 194, 269, 270
BsaWI WCCGGW 1 cut(s) 123
BsaXI ACNNNNNCTCC 2 cut(s) 337, 367
Bsc4I CCNNNNNNNGG 1 cut(s) 396
Bse1I ACTGG 2 cut(s) 166, 462
BseAI TCCGGA 1 cut(s) 123
BseCI ATCGAT 1 cut(s) 426
BseDI CCNNGG 3 cut(s) 194, 269, 270
BseLI CCNNNNNNNGG 1 cut(s) 396
BseMII CTCAG 2 cut(s) 78, 198
BseNI ACTGG 2 cut(s) 166, 462
BseSI GKGCMC 1 cut(s) 303
Bsh1236I CGCG 1 cut(s) 367
BshFI GGCC 1 cut(s) 117
BshNI GGYRCC 1 cut(s) 83
BshVI ATCGAT 1 cut(s) 426
BsiHKAI GWGCWC 1 cut(s) 303
BsiHKCI CYCGRG 2 cut(s) 107, 269
BsiSI CCGG 2 cut(s) 124, 270
BslI CCNNNNNNNGG 1 cut(s) 396
BsnI GGCC 1 cut(s) 117
BsoBI CYCGRG 2 cut(s) 107, 269
Bsp119I TTCGAA 1 cut(s) 238
Bsp1286I GDGCHC 1 cut(s) 303
Bsp13I TCCGGA 1 cut(s) 123
Bsp143I GATC 3 cut(s) 190, 313, 445
BspACI CCGC 2 cut(s) 114, 365
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 2 cut(s) 77, 197
BspDI ATCGAT 1 cut(s) 426
BspEI TCCGGA 1 cut(s) 123
BspFNI CGCG 1 cut(s) 367
BspLI GGNNCC 1 cut(s) 85
BspOI GCTAGC 1 cut(s) 206
BspPI GGATC 1 cut(s) 185
BspT104I TTCGAA 1 cut(s) 238
BspT107I GGYRCC 1 cut(s) 83
BsrBI CCGCTC 1 cut(s) 114
BsrI ACTGG 2 cut(s) 166, 462
BssECI CCNNGG 3 cut(s) 194, 269, 270
BssMI GATC 3 cut(s) 190, 313, 445
BssNI GRCGYC 1 cut(s) 84
BssT1I CCWWGG 1 cut(s) 194
Bst4CI ACNGT 1 cut(s) 252
Bst6I CTCTTC 1 cut(s) 376
BstACI GRCGYC 1 cut(s) 84
BstBI TTCGAA 1 cut(s) 238
BstC8I GCNNGC 1 cut(s) 204
BstDEI CTNAG 3 cut(s) 64, 184, 350
BstFNI CGCG 1 cut(s) 367
BstH2I RGCGCY 2 cut(s) 87, 209
BstHHI GCGC 2 cut(s) 86, 208
BstKTI GATC 3 cut(s) 193, 316, 448
BstMBI GATC 3 cut(s) 190, 313, 445
BstSCI CCNGG 2 cut(s) 268, 269
BstSFI CTRYAG 1 cut(s) 157
BstSLI GKGCMC 1 cut(s) 303
BstUI CGCG 1 cut(s) 367
Bsu15I ATCGAT 1 cut(s) 426
BsuRI GGCC 1 cut(s) 117
BsuTUI ATCGAT 1 cut(s) 426
BtrI CACGTC 1 cut(s) 298
BtsI GCAGTG 1 cut(s) 101
BtsIMutI CAGTG 2 cut(s) 101, 469
Cac8I GCNNGC 1 cut(s) 204
CfoI GCGC 2 cut(s) 86, 208
Cfr13I GGNCC 1 cut(s) 420
Cfr9I CCCGGG 1 cut(s) 269
ClaI ATCGAT 1 cut(s) 426
Csp6I GTAC 1 cut(s) 48
CviJI RGCY 6 cut(s) 10, 55, 63, 117, 400, 468
CviKI_1 RGCY 6 cut(s) 10, 55, 63, 117, 400, 468
CviQI GTAC 1 cut(s) 48
DdeI CTNAG 3 cut(s) 64, 184, 350
DinI GGCGCC 1 cut(s) 85
DpnI GATC 3 cut(s) 192, 315, 447
DpnII GATC 3 cut(s) 190, 313, 445
Eam1104I CTCTTC 1 cut(s) 376
EarI CTCTTC 1 cut(s) 376
Eco130I CCWWGG 1 cut(s) 194
Eco32I GATATC 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 420
Eco47III AGCGCT 1 cut(s) 207
Eco88I CYCGRG 2 cut(s) 107, 269
EcoRI GAATTC 1 cut(s) 215
EcoRV GATATC 1 cut(s) 20
EcoT14I CCWWGG 1 cut(s) 194
EgeI GGCGCC 1 cut(s) 85
EheI GGCGCC 1 cut(s) 85
ErhI CCWWGG 1 cut(s) 194
FaiI YATR 2 cut(s) 98, 143
FbaI TGATCA 2 cut(s) 313, 445
FblI GTMKAC 1 cut(s) 30
Fnu4HI GCNGC 1 cut(s) 115
Fsp4HI GCNGC 1 cut(s) 115
FspBI CTAG 1 cut(s) 203
GlaI GCGC 2 cut(s) 85, 207
GluI GCNGC 1 cut(s) 115
HaeII RGCGCY 2 cut(s) 87, 209
HaeIII GGCC 1 cut(s) 117
HapII CCGG 2 cut(s) 124, 270
HhaI GCGC 2 cut(s) 86, 208
Hin1I GRCGYC 1 cut(s) 84
Hin6I GCGC 2 cut(s) 84, 206
HinP1I GCGC 2 cut(s) 84, 206
HinfI GANTC 4 cut(s) 162, 346, 386, 408
HpaII CCGG 2 cut(s) 124, 270
HphI GGTGA 1 cut(s) 158
Hpy166II GTNNAC 2 cut(s) 31, 301
Hpy188I TCNGA 2 cut(s) 333, 391
Hpy188III TCNNGA 6 cut(s) 66, 109, 124, 256, 278, 311
Hpy8I GTNNAC 2 cut(s) 31, 301
HpyAV CCTTC 1 cut(s) 8
HpyCH4III ACNGT 1 cut(s) 252
HpyCH4IV ACGT 1 cut(s) 297
HpyCH4V TGCA 3 cut(s) 41, 301, 340
HpyF3I CTNAG 3 cut(s) 64, 184, 350
HpySE526I ACGT 1 cut(s) 297
Hsp92I GRCGYC 1 cut(s) 84
HspAI GCGC 2 cut(s) 84, 206
KasI GGCGCC 1 cut(s) 83
Kpn2I TCCGGA 1 cut(s) 123
Ksp22I TGATCA 2 cut(s) 313, 445
Kzo9I GATC 3 cut(s) 190, 313, 445
LpnPI CCDG 8 cut(s) 24, 51, 137, 179, 194, 263, 283, 324
MaeI CTAG 1 cut(s) 203
MaeII ACGT 1 cut(s) 297
MaeIII GTNAC 1 cut(s) 99
MalI GATC 3 cut(s) 192, 315, 447
MbiI CCGCTC 1 cut(s) 114
MboI GATC 3 cut(s) 190, 313, 445
MboII GAAGA 3 cut(s) 70, 252, 393
MhlI GDGCHC 1 cut(s) 303
MluCI AATT 3 cut(s) 174, 215, 288
Mly113I GGCGCC 1 cut(s) 84
MlyI GAGTC 1 cut(s) 355
MnlI CCTC 1 cut(s) 390
MroI TCCGGA 1 cut(s) 123
MseI TTAA 1 cut(s) 287
MspI CCGG 2 cut(s) 124, 270
MspR9I CCNGG 2 cut(s) 270, 271
MvnI CGCG 1 cut(s) 367
NarI GGCGCC 1 cut(s) 84
NciI CCSGG 2 cut(s) 270, 271
NdeII GATC 3 cut(s) 190, 313, 445
NheI GCTAGC 1 cut(s) 202
NlaIV GGNNCC 1 cut(s) 85
NmuCI GTSAC 1 cut(s) 99
NspV TTCGAA 1 cut(s) 238
PaeR7I CTCGAG 1 cut(s) 107
PfeI GAWTC 3 cut(s) 162, 386, 408
PkrI GCNGC 1 cut(s) 116
PleI GAGTC 1 cut(s) 354
PluTI GGCGCC 1 cut(s) 87
PpsI GAGTC 1 cut(s) 354
PspN4I GGNNCC 1 cut(s) 85
PspPI GGNCC 1 cut(s) 420
RsaI GTAC 1 cut(s) 49
RsaNI GTAC 1 cut(s) 48
SaqAI TTAA 1 cut(s) 287
SatI GCNGC 1 cut(s) 115
Sau3AI GATC 3 cut(s) 190, 313, 445
Sau96I GGNCC 1 cut(s) 420
SchI GAGTC 1 cut(s) 355
ScrFI CCNGG 2 cut(s) 270, 271
SduI GDGCHC 1 cut(s) 303
SetI ASST 5 cut(s) 65, 148, 200, 300, 417
SfcI CTRYAG 1 cut(s) 157
SfoI GGCGCC 1 cut(s) 85
Sfr274I CTCGAG 1 cut(s) 107
SfuI TTCGAA 1 cut(s) 238
SinI GGWCC 1 cut(s) 420
SlaI CTCGAG 1 cut(s) 107
SmaI CCCGGG 1 cut(s) 271
SmlI CTYRAG 2 cut(s) 107, 401
SmoI CTYRAG 2 cut(s) 107, 401
Sse9I AATT 3 cut(s) 174, 215, 288
SsiI CCGC 2 cut(s) 114, 365
SspDI GGCGCC 1 cut(s) 83
SspI AATATT 1 cut(s) 264
SspMI CTAG 1 cut(s) 203
StyD4I CCNGG 2 cut(s) 268, 269
StyI CCWWGG 1 cut(s) 194
TaaI ACNGT 1 cut(s) 252
TaiI ACGT 1 cut(s) 300
TaqI TCGA 4 cut(s) 108, 238, 426, 470
TasI AATT 3 cut(s) 174, 215, 288
TauI GCSGC 1 cut(s) 117
TfiI GAWTC 3 cut(s) 162, 386, 408
Tru1I TTAA 1 cut(s) 287
Tru9I TTAA 1 cut(s) 287
TscAI CASTG 2 cut(s) 108, 469
TseFI GTSAC 1 cut(s) 99
Tsp45I GTSAC 1 cut(s) 99
TspDTI ATGAA 1 cut(s) 63
TspGWI ACGGA 1 cut(s) 358
TspMI CCCGGG 1 cut(s) 269
TspRI CASTG 2 cut(s) 108, 469
VneI GTGCAC 1 cut(s) 299
VpaK11BI GGWCC 1 cut(s) 420
XapI RAATTY 1 cut(s) 215
XcmI CCANNNNNNNNNTGG 1 cut(s) 173
XhoI CTCGAG 1 cut(s) 107
XmaI CCCGGG 1 cut(s) 269
XmiI GTMKAC 1 cut(s) 30
XspI CTAG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.