Rmu_sc0001214.1_g000027

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001214.1
OG0008763
Physical Location & Seq
Forward (+)
81352 .. 81852
501 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001214.1_g000027.1.cds

Sequence Viewer

Length: 501 bp
atggaaaggccagaagggtatccagtagtagatgctacagaaatcgatcagccatcaaactccggcgcatcatttctcagcaccaagaatgatatgtcacaactagaaagcggtctacttcgaagaaactacagcgacgtgggtgaaaaaaagtactacagattccaatcaaaagttggtaagttacttgatccgaggttgctagcgttggtagagttcttcagggagctctacttccgaagactcgagctgttccagaaaatatttccggggcgtcccgataagtttctggaggagataccaaagaaactgaatttggtttctcagacgaccaagagtcggaccatgcaaaggagtctgagcgttggctcgccgcgtgaattcaaaccagacgagtcggaaatgaggctggagcgattcaaggtcagaactattgatgtggatgatgatgttgaacaaggtgtgcaggacgataaaaccaagtccgggggctcaaagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

166

Amino Acids

19.24

Weight (kDa)

8.73

Isoelectric Point (pI)

39.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 115
AccII CGCG 1 cut(s) 376
AciI CCGC 2 cut(s) 111, 374
AclWI GGATC 1 cut(s) 185
AcsI RAATTY 2 cut(s) 313, 380
AcuI CTGAAG 1 cut(s) 205
AcyI GRCGYC 1 cut(s) 274
AfaI GTAC 1 cut(s) 155
AfiI CCNNNNNNNGG 3 cut(s) 339, 351, 486
AgsI TTSAA 3 cut(s) 385, 421, 455
AhdI GACNNNNNGTC 1 cut(s) 336
AjiI CACGTC 1 cut(s) 139
AjuI GAANNNNNNNTTGG 2 cut(s) 299, 331
AluBI AGCT 2 cut(s) 229, 250
AluI AGCT 2 cut(s) 229, 250
Alw21I GWGCWC 1 cut(s) 231
AlwI GGATC 1 cut(s) 185
Ama87I CYCGRG 1 cut(s) 245
AoxI GGCC 1 cut(s) 8
ApoI RAATTY 2 cut(s) 313, 380
AspLEI GCGC 1 cut(s) 68
AspS9I GGNCC 1 cut(s) 342
AsuC2I CCSGG 2 cut(s) 270, 487
AsuHPI GGTGA 1 cut(s) 155
AsuII TTCGAA 1 cut(s) 121
AsuNHI GCTAGC 1 cut(s) 202
AvaI CYCGRG 1 cut(s) 245
AvaII GGWCC 1 cut(s) 342
BanII GRGCYC 2 cut(s) 231, 494
BbsI GAAGAC 1 cut(s) 247
Bbv12I GWGCWC 1 cut(s) 231
BccI CCATC 1 cut(s) 61
BciVI GTATCC 1 cut(s) 30
BcnI CCSGG 2 cut(s) 270, 487
BfaI CTAG 2 cut(s) 104, 203
BfmI CTRYAG 3 cut(s) 36, 130, 157
BfuI GTATCC 1 cut(s) 30
BisI GCNGC 1 cut(s) 374
BlsI GCNGC 1 cut(s) 375
BmcAI AGTACT 1 cut(s) 155
Bme1390I CCNGG 2 cut(s) 270, 487
Bme18I GGWCC 1 cut(s) 342
BmeRI GACNNNNNGTC 1 cut(s) 336
BmeT110I CYCGRG 1 cut(s) 245
BmgBI CACGTC 1 cut(s) 139
BmgT120I GGNCC 1 cut(s) 342
BmrFI CCNGG 2 cut(s) 270, 487
BmsI GCATC 2 cut(s) 22, 77
BmtI GCTAGC 1 cut(s) 206
BpiI GAAGAC 1 cut(s) 247
BpmI CTGGAG 2 cut(s) 311, 431
Bpu14I TTCGAA 1 cut(s) 121
BpuMI CCSGG 2 cut(s) 270, 487
Bsa29I ATCGAT 1 cut(s) 45
BsaBI GATNNNNATC 1 cut(s) 166
BsaHI GRCGYC 1 cut(s) 274
BsaJI CCNNGG 3 cut(s) 194, 269, 486
Bsc4I CCNNNNNNNGG 3 cut(s) 339, 351, 486
Bse1I ACTGG 1 cut(s) 23
Bse8I GATNNNNATC 1 cut(s) 166
BseCI ATCGAT 1 cut(s) 45
BseDI CCNNGG 3 cut(s) 194, 269, 486
BseGI GGATG 1 cut(s) 448
BseJI GATNNNNATC 1 cut(s) 166
BseLI CCNNNNNNNGG 3 cut(s) 339, 351, 486
BseMII CTCAG 3 cut(s) 91, 338, 350
BseNI ACTGG 1 cut(s) 23
BseRI GAGGAG 1 cut(s) 308
BsgI GTGCAG 1 cut(s) 485
Bsh1236I CGCG 1 cut(s) 376
BshFI GGCC 1 cut(s) 10
BshVI ATCGAT 1 cut(s) 45
BsiHKAI GWGCWC 1 cut(s) 231
BsiHKCI CYCGRG 1 cut(s) 245
BsiSI CCGG 3 cut(s) 63, 269, 486
BslFI GGGAC 1 cut(s) 261
BslI CCNNNNNNNGG 3 cut(s) 339, 351, 486
BsmFI GGGAC 1 cut(s) 261
BsnI GGCC 1 cut(s) 10
BsoBI CYCGRG 1 cut(s) 245
Bsp119I TTCGAA 1 cut(s) 121
Bsp1286I GDGCHC 2 cut(s) 231, 494
Bsp143I GATC 2 cut(s) 46, 190
BspACI CCGC 2 cut(s) 111, 374
BspANI GGCC 1 cut(s) 10
BspCNI CTCAG 3 cut(s) 90, 337, 351
BspDI ATCGAT 1 cut(s) 45
BspFNI CGCG 1 cut(s) 376
BspOI GCTAGC 1 cut(s) 206
BspPI GGATC 1 cut(s) 185
BspT104I TTCGAA 1 cut(s) 121
BsrI ACTGG 1 cut(s) 23
BssECI CCNNGG 3 cut(s) 194, 269, 486
BssMI GATC 2 cut(s) 46, 190
BssNI GRCGYC 1 cut(s) 274
BstACI GRCGYC 1 cut(s) 274
BstBI TTCGAA 1 cut(s) 121
BstC8I GCNNGC 2 cut(s) 204, 371
BstDEI CTNAG 3 cut(s) 77, 324, 359
BstF5I GGATG 1 cut(s) 448
BstFNI CGCG 1 cut(s) 376
BstHHI GCGC 1 cut(s) 68
BstKTI GATC 2 cut(s) 49, 193
BstMBI GATC 2 cut(s) 46, 190
BstSCI CCNGG 2 cut(s) 268, 485
BstSFI CTRYAG 3 cut(s) 36, 130, 157
BstUI CGCG 1 cut(s) 376
BstV2I GAAGAC 1 cut(s) 247
Bsu15I ATCGAT 1 cut(s) 45
BsuI GTATCC 1 cut(s) 30
BsuRI GGCC 1 cut(s) 10
BsuTUI ATCGAT 1 cut(s) 45
BtrI CACGTC 1 cut(s) 139
BtsCI GGATG 1 cut(s) 448
Cac8I GCNNGC 2 cut(s) 204, 371
CfoI GCGC 1 cut(s) 68
Cfr13I GGNCC 1 cut(s) 342
ClaI ATCGAT 1 cut(s) 45
CseI GACGC 1 cut(s) 263
Csp6I GTAC 1 cut(s) 154
CviAII CATG 1 cut(s) 346
CviJI RGCY 7 cut(s) 10, 52, 229, 250, 369, 409, 492
CviKI_1 RGCY 7 cut(s) 10, 52, 229, 250, 369, 409, 492
CviQI GTAC 1 cut(s) 154
DdeI CTNAG 3 cut(s) 77, 324, 359
DpnI GATC 2 cut(s) 48, 192
DpnII GATC 2 cut(s) 46, 190
DriI GACNNNNNGTC 1 cut(s) 336
Eam1105I GACNNNNNGTC 1 cut(s) 336
Ecl136II GAGCTC 1 cut(s) 229
Eco24I GRGCYC 2 cut(s) 231, 494
Eco47I GGWCC 1 cut(s) 342
Eco53kI GAGCTC 1 cut(s) 229
Eco57I CTGAAG 1 cut(s) 205
Eco88I CYCGRG 1 cut(s) 245
EcoICRI GAGCTC 1 cut(s) 229
EcoRI GAATTC 1 cut(s) 380
EcoT38I GRGCYC 2 cut(s) 231, 494
FaeI CATG 1 cut(s) 349
FaiI YATR 2 cut(s) 95, 347
FaqI GGGAC 1 cut(s) 261
FatI CATG 1 cut(s) 345
FblI GTMKAC 1 cut(s) 115
Fnu4HI GCNGC 1 cut(s) 374
FokI GGATG 1 cut(s) 455
FriOI GRGCYC 2 cut(s) 231, 494
Fsp4HI GCNGC 1 cut(s) 374
FspBI CTAG 2 cut(s) 104, 203
GlaI GCGC 1 cut(s) 67
GluI GCNGC 1 cut(s) 374
GsuI CTGGAG 2 cut(s) 311, 431
HaeIII GGCC 1 cut(s) 10
HapII CCGG 3 cut(s) 63, 269, 486
HgaI GACGC 1 cut(s) 263
HhaI GCGC 1 cut(s) 68
Hin1I GRCGYC 1 cut(s) 274
Hin1II CATG 1 cut(s) 349
Hin6I GCGC 1 cut(s) 66
HinP1I GCGC 1 cut(s) 66
HinfI GANTC 6 cut(s) 162, 243, 337, 355, 395, 417
HpaII CCGG 3 cut(s) 63, 269, 486
HphI GGTGA 1 cut(s) 155
Hpy166II GTNNAC 1 cut(s) 116
Hpy188I TCNGA 7 cut(s) 195, 239, 327, 342, 360, 400, 428
Hpy188III TCNNGA 3 cut(s) 256, 278, 290
Hpy8I GTNNAC 1 cut(s) 116
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 1 cut(s) 8
HpyCH4IV ACGT 1 cut(s) 138
HpyCH4V TGCA 2 cut(s) 349, 466
HpyF3I CTNAG 3 cut(s) 77, 324, 359
HpySE526I ACGT 1 cut(s) 138
Hsp92I GRCGYC 1 cut(s) 274
Hsp92II CATG 1 cut(s) 349
HspAI GCGC 1 cut(s) 66
Kzo9I GATC 2 cut(s) 46, 190
LmnI GCTCC 2 cut(s) 226, 412
LweI GCATC 2 cut(s) 22, 77
MaeI CTAG 2 cut(s) 104, 203
MaeII ACGT 1 cut(s) 138
MaeIII GTNAC 2 cut(s) 96, 183
MalI GATC 2 cut(s) 48, 192
MboI GATC 2 cut(s) 46, 190
MboII GAAGA 3 cut(s) 135, 211, 252
MhlI GDGCHC 2 cut(s) 231, 494
MluCI AATT 2 cut(s) 313, 380
MlyI GAGTC 4 cut(s) 237, 346, 364, 404
MmeI TCCRAC 2 cut(s) 320, 378
MnlI CCTC 3 cut(s) 189, 286, 399
MspI CCGG 3 cut(s) 63, 269, 486
MspR9I CCNGG 2 cut(s) 270, 487
MvnI CGCG 1 cut(s) 376
NciI CCSGG 2 cut(s) 270, 487
NdeII GATC 2 cut(s) 46, 190
NheI GCTAGC 1 cut(s) 202
NlaIII CATG 1 cut(s) 349
NmuCI GTSAC 1 cut(s) 96
NspV TTCGAA 1 cut(s) 121
PaeR7I CTCGAG 1 cut(s) 245
PfeI GAWTC 2 cut(s) 162, 417
PkrI GCNGC 1 cut(s) 375
PleI GAGTC 4 cut(s) 237, 345, 363, 403
PpsI GAGTC 4 cut(s) 237, 345, 363, 403
Psp124BI GAGCTC 1 cut(s) 231
PspPI GGNCC 1 cut(s) 342
PspXI VCTCGAGB 1 cut(s) 245
RsaI GTAC 1 cut(s) 155
RsaNI GTAC 1 cut(s) 154
SacI GAGCTC 1 cut(s) 231
SatI GCNGC 1 cut(s) 374
Sau3AI GATC 2 cut(s) 46, 190
Sau96I GGNCC 1 cut(s) 342
ScaI AGTACT 1 cut(s) 155
SchI GAGTC 4 cut(s) 237, 346, 364, 404
ScrFI CCNGG 2 cut(s) 270, 487
SduI GDGCHC 2 cut(s) 231, 494
SetI ASST 6 cut(s) 141, 200, 231, 252, 426, 463
SfaNI GCATC 2 cut(s) 22, 77
SfcI CTRYAG 3 cut(s) 36, 130, 157
Sfr274I CTCGAG 1 cut(s) 245
SfuI TTCGAA 1 cut(s) 121
SinI GGWCC 1 cut(s) 342
SlaI CTCGAG 1 cut(s) 245
SmlI CTYRAG 1 cut(s) 245
SmoI CTYRAG 1 cut(s) 245
Sse9I AATT 2 cut(s) 313, 380
SsiI CCGC 2 cut(s) 111, 374
SspI AATATT 1 cut(s) 264
SspMI CTAG 2 cut(s) 104, 203
SstI GAGCTC 1 cut(s) 231
StyD4I CCNGG 2 cut(s) 268, 485
TaiI ACGT 1 cut(s) 141
TaqI TCGA 3 cut(s) 45, 121, 246
TasI AATT 2 cut(s) 313, 380
TatI WGTACW 1 cut(s) 153
TauI GCSGC 1 cut(s) 376
TfiI GAWTC 2 cut(s) 162, 417
TseFI GTSAC 1 cut(s) 96
Tsp45I GTSAC 1 cut(s) 96
VpaK11BI GGWCC 1 cut(s) 342
XapI RAATTY 2 cut(s) 313, 380
XcmI CCANNNNNNNNNTGG 1 cut(s) 173
XhoI CTCGAG 1 cut(s) 245
XmiI GTMKAC 1 cut(s) 115
XspI CTAG 2 cut(s) 104, 203
ZrmI AGTACT 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.