MD07G1162400.v1.1

B3 domain-containing transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0011010
Physical Location & Seq
Forward (+)
23751189 .. 23753308
2120 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1162400.v1.1.491

Sequence Viewer

Length: 1323 bp
ATGGAATTTATGCAAAGAGATCAAAAGGGGTATTCTGACAAAGAAGATCAAGATCAAGAACAAGAAGATCAAGAGGAAGAGGAGGATATAATCACCACCAGTAAGTTTTCTTCATCATCATCTCATACTTCAAATAACAGCAATTATGGGTCCTCACCAAAGTACAAAGCCATCCTTTTTCCTCCTCCTCCTGACCACCACCAGCAGTACGGCCAGCAAAAGCCGCGGCTTAATATTCAGAATCACGGCTCTCCGGAAGCCACAAACTTCATGGACTTATCACTAAACAAAGATATTCAGGCTGCTGAGGGTGGTACTAACGAAAATTACTGGCCTGCGAGCTGCGAAAGGGAGCACATGTTTGAGAAGGTTGTGACGCCGAGCGATGTAGGGAAGCTCAACCGCCTAGTTATTCCGAAGCAGCATGCCGAGCGGTTCTTCCCGCTGGACTCATCATCAAACGACAATGGATTGTTTTTGAATTTTCAAGACCGGACGGGGAAGCCATGGCGATTTAGGTACTCTTATTGGAATAGCAGCCAGAGCTATGTCATAACGAAAGGGTGGAGCCGTTTTGTGAAGGAGAAGAAGCTGGATGCCGGCGACATTGTGTCGTTTGAGCGCGGGGTTGGGGAATCCGGTAAGGACAGATTGTTCATTGACTGGAAGGGGCGCCCTCCCCCTCCTCCTCCACCTCGGCTACATCATCATAACCATTACGCTGGAGGCAGGTTTTGCTCGCTGCTGCAGAGGCCGCCGCCTTCGTCTGTGATATCGTCTATGCCTCTTAGACACCACGAACATTTTCTTCATTCGAACAACAACATTCATCATCCGTACCACCATCACCACCATAACCATCAGATCATGAATCAACAATATTTACCGCATCAGATTCATCAGTTGCAAGGTGGAAGAGGTCATCACGTGATTCATCATCAATATCCAAGGGTTATCGATTCGGTTCCGCTTTATCAGCATGCTAATTATAGGAGTTCTACTACTAGTACTAGTAGTGAAAGTGCTAGTGTTGCTTCAGCTGGTAAAAAGCTTAGGCTTTTTGGTGTGAACATGGAATGCTCAAGTAGTCCAACTGAGAAGGAGGATCATCAAGAATGTCAGATATTGTCTTCAACAGCAATACCTTACCATCATCATCATCATAATCTTCCATCGTCAACGTCTTCGCTGGCTTCTGCTCCGTTTCAATTCATGAGGCTGCCCAATACAAGTAGTACTAGTAGTACTCCACCAGACCATCACTTCCTCTCAAACAAAGGGAAATCTACAACTACTTCGTTGTCGTTTTATTTGAATCCCTAA

Protein Analysis

441

Amino Acids

50.3

Weight (kDa)

7.28

Isoelectric Point (pI)

52.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 121 - 209 1.6e-24 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 720
AccB1I GGYRCC 1 cut(s) 672
AccBSI CCGCTC 1 cut(s) 433
AccII CGCG 2 cut(s) 226, 624
AccIII TCCGGA 1 cut(s) 253
AclWI GGATC 1 cut(s) 1113
AcoI YGGCCR 1 cut(s) 211
AcsI RAATTY 2 cut(s) 5, 481
AcuI CTGAAG 1 cut(s) 1020
AcvI CACGTG 1 cut(s) 928
AcyI GRCGYC 2 cut(s) 377, 673
AfaI GTAC 8 cut(s) 164, 209, 316, 521, 839, 1009, 1237, 1246
AflIII ACRYGT 1 cut(s) 357
AgsI TTSAA 6 cut(s) 132, 481, 488, 1134, 1208, 1315
AhdI GACNNNNNGTC 1 cut(s) 610
AhlI ACTAGT 3 cut(s) 1004, 1010, 1238
AloI GAACNNNNNNTCC 2 cut(s) 638, 670
AluBI AGCT 6 cut(s) 342, 397, 546, 592, 1040, 1051
AluI AGCT 6 cut(s) 342, 397, 546, 592, 1040, 1051
Alw21I GWGCWC 1 cut(s) 357
AlwI GGATC 1 cut(s) 1113
Aor13HI TCCGGA 1 cut(s) 253
AoxI GGCC 3 cut(s) 211, 332, 752
ApeKI GCWGC 7 cut(s) 302, 342, 421, 537, 742, 745, 1219
ApoI RAATTY 2 cut(s) 5, 481
Asp700I GAANNNNTTC 1 cut(s) 804
AspLEI GCGC 2 cut(s) 624, 675
AspS9I GGNCC 1 cut(s) 150
AsuHPI GGTGA 3 cut(s) 85, 147, 839
AsuII TTCGAA 1 cut(s) 815
AvaII GGWCC 1 cut(s) 150
BanI GGYRCC 1 cut(s) 672
BarI GAAGNNNNNNTAC 2 cut(s) 94, 126
BbrPI CACGTG 1 cut(s) 928
BbsI GAAGAC 2 cut(s) 1122, 1176
Bbv12I GWGCWC 1 cut(s) 357
BbvCI CCTCAGC 1 cut(s) 306
BbvI GCAGC 7 cut(s) 289, 329, 433, 549, 729, 732, 1206
BccI CCATC 6 cut(s) 179, 852, 867, 1158, 1180, 1266
BceAI ACGGC 3 cut(s) 226, 262, 555
BcuI ACTAGT 3 cut(s) 1004, 1010, 1238
BfaI CTAG 5 cut(s) 407, 1005, 1011, 1026, 1239
BfmI CTRYAG 1 cut(s) 746
BfoI RGCGCY 1 cut(s) 676
BfuAI ACCTGC 1 cut(s) 720
BmcAI AGTACT 3 cut(s) 1009, 1237, 1246
Bme18I GGWCC 1 cut(s) 150
BmeRI GACNNNNNGTC 1 cut(s) 610
BmgT120I GGNCC 1 cut(s) 150
BmiI GGNNCC 4 cut(s) 151, 569, 674, 966
BmsI GCATC 2 cut(s) 586, 898
BpiI GAAGAC 2 cut(s) 1122, 1176
BpmI CTGGAG 1 cut(s) 744
Bpu10I CCTNAGC 2 cut(s) 306, 1052
Bpu14I TTCGAA 1 cut(s) 815
BpuEI CTTGAG 1 cut(s) 1066
Bsa29I ATCGAT 1 cut(s) 957
BsaAI YACGTR 1 cut(s) 928
BsaBI GATNNNNATC 1 cut(s) 51
BsaHI GRCGYC 2 cut(s) 377, 673
BsaJI CCNNGG 4 cut(s) 224, 506, 695, 947
BsaWI WCCGGW 3 cut(s) 253, 492, 638
BsaXI ACNNNNNCTCC 2 cut(s) 344, 374
Bse118I RCCGGY 1 cut(s) 599
Bse1I ACTGG 3 cut(s) 99, 335, 668
Bse8I GATNNNNATC 1 cut(s) 51
BseAI TCCGGA 1 cut(s) 253
BseCI ATCGAT 1 cut(s) 957
BseDI CCNNGG 4 cut(s) 224, 506, 695, 947
BseGI GGATG 3 cut(s) 171, 601, 832
BseJI GATNNNNATC 1 cut(s) 51
BseMII CTCAG 2 cut(s) 297, 1086
BseNI ACTGG 3 cut(s) 99, 335, 668
BseRI GAGGAG 5 cut(s) 95, 174, 177, 675, 678
BseXI GCAGC 7 cut(s) 289, 329, 433, 549, 729, 732, 1206
Bsh1236I CGCG 2 cut(s) 226, 624
BshFI GGCC 3 cut(s) 213, 334, 754
BshNI GGYRCC 1 cut(s) 672
BshVI ATCGAT 1 cut(s) 957
BsiHKAI GWGCWC 1 cut(s) 357
BsiSI CCGG 4 cut(s) 254, 493, 600, 639
BsmI GAATGC 1 cut(s) 1082
BsnI GGCC 3 cut(s) 213, 334, 754
Bsp119I TTCGAA 1 cut(s) 815
Bsp1286I GDGCHC 1 cut(s) 357
Bsp13I TCCGGA 1 cut(s) 253
Bsp143I GATC 6 cut(s) 19, 46, 52, 67, 864, 1105
Bsp19I CCATGG 1 cut(s) 506
BspANI GGCC 3 cut(s) 213, 334, 754
BspCNI CTCAG 2 cut(s) 298, 1087
BspDI ATCGAT 1 cut(s) 957
BspEI TCCGGA 1 cut(s) 253
BspFNI CGCG 2 cut(s) 226, 624
BspHI TCATGA 2 cut(s) 867, 1212
BspLI GGNNCC 4 cut(s) 151, 569, 674, 966
BspMAI CTGCAG 1 cut(s) 750
BspMI ACCTGC 1 cut(s) 720
BspPI GGATC 1 cut(s) 1113
BspT104I TTCGAA 1 cut(s) 815
BspT107I GGYRCC 1 cut(s) 672
BsrBI CCGCTC 1 cut(s) 433
BsrFI RCCGGY 1 cut(s) 599
BsrI ACTGG 3 cut(s) 99, 335, 668
BssAI RCCGGY 1 cut(s) 599
BssECI CCNNGG 4 cut(s) 224, 506, 695, 947
BssMI GATC 6 cut(s) 19, 46, 52, 67, 864, 1105
BssNI GRCGYC 2 cut(s) 377, 673
BssT1I CCWWGG 2 cut(s) 506, 947
Bst6I CTCTTC 2 cut(s) 72, 910
BstACI GRCGYC 2 cut(s) 377, 673
BstAPI GCANNNNNTGC 1 cut(s) 735
BstBAI YACGTR 1 cut(s) 928
BstBI TTCGAA 1 cut(s) 815
BstC8I GCNNGC 8 cut(s) 215, 336, 340, 426, 601, 740, 981, 1191
BstDEI CTNAG 4 cut(s) 306, 788, 1052, 1095
BstDSI CCRYGG 2 cut(s) 224, 506
BstF5I GGATG 3 cut(s) 171, 601, 832
BstFNI CGCG 2 cut(s) 226, 624
BstH2I RGCGCY 1 cut(s) 676
BstHHI GCGC 2 cut(s) 624, 675
BstKTI GATC 6 cut(s) 22, 49, 55, 70, 867, 1108
BstMBI GATC 6 cut(s) 19, 46, 52, 67, 864, 1105
BstMWI GCNNNNNNNGC 8 cut(s) 223, 430, 543, 735, 751, 754, 976, 1031
BstNSI RCATGY 3 cut(s) 361, 428, 983
BstSFI CTRYAG 1 cut(s) 746
BstUI CGCG 2 cut(s) 226, 624
BstV1I GCAGC 7 cut(s) 289, 329, 433, 549, 729, 732, 1206
BstV2I GAAGAC 2 cut(s) 1122, 1176
BstXI CCANNNNNNTGG 1 cut(s) 722
Bsu15I ATCGAT 1 cut(s) 957
BsuRI GGCC 3 cut(s) 213, 334, 754
BsuTUI ATCGAT 1 cut(s) 957
BtgI CCRYGG 2 cut(s) 224, 506
BtgZI GCGATG 1 cut(s) 399
BtsCI GGATG 3 cut(s) 171, 601, 832
BveI ACCTGC 1 cut(s) 720
Cac8I GCNNGC 8 cut(s) 215, 336, 340, 426, 601, 740, 981, 1191
CciI TCATGA 2 cut(s) 867, 1212
CfoI GCGC 2 cut(s) 624, 675
Cfr10I RCCGGY 1 cut(s) 599
Cfr13I GGNCC 1 cut(s) 150
Cfr42I CCGCGG 1 cut(s) 227
ClaI ATCGAT 1 cut(s) 957
CseI GACGC 1 cut(s) 385
Csp6I GTAC 8 cut(s) 163, 208, 315, 520, 838, 1008, 1236, 1245
CviAII CATG 8 cut(s) 271, 358, 425, 507, 868, 980, 1072, 1213
CviQI GTAC 8 cut(s) 163, 208, 315, 520, 838, 1008, 1236, 1245
DdeI CTNAG 4 cut(s) 306, 788, 1052, 1095
DinI GGCGCC 1 cut(s) 674
DpnI GATC 6 cut(s) 21, 48, 54, 69, 866, 1107
DpnII GATC 6 cut(s) 19, 46, 52, 67, 864, 1105
DriI GACNNNNNGTC 1 cut(s) 610
EaeI YGGCCR 1 cut(s) 211
Eam1104I CTCTTC 2 cut(s) 72, 910
Eam1105I GACNNNNNGTC 1 cut(s) 610
EarI CTCTTC 2 cut(s) 72, 910
Eco130I CCWWGG 2 cut(s) 506, 947
Eco32I GATATC 1 cut(s) 774
Eco47I GGWCC 1 cut(s) 150
Eco57I CTGAAG 1 cut(s) 1020
Eco72I CACGTG 1 cut(s) 928
EcoO109I RGGNCCY 1 cut(s) 150
EcoRV GATATC 1 cut(s) 774
EcoT14I CCWWGG 2 cut(s) 506, 947
EgeI GGCGCC 1 cut(s) 674
EheI GGCGCC 1 cut(s) 674
ErhI CCWWGG 2 cut(s) 506, 947
FaeI CATG 8 cut(s) 274, 361, 428, 510, 871, 983, 1075, 1216
FalI AAGNNNNNCTT 2 cut(s) 159, 191
FatI CATG 8 cut(s) 270, 357, 424, 506, 867, 979, 1071, 1212
FauI CCCGC 2 cut(s) 450, 617
FokI GGATG 3 cut(s) 158, 608, 819
FspBI CTAG 5 cut(s) 407, 1005, 1011, 1026, 1239
GlaI GCGC 2 cut(s) 623, 674
GsuI CTGGAG 1 cut(s) 744
HaeII RGCGCY 1 cut(s) 676
HaeIII GGCC 3 cut(s) 213, 334, 754
HapII CCGG 4 cut(s) 254, 493, 600, 639
HgaI GACGC 1 cut(s) 385
HhaI GCGC 2 cut(s) 624, 675
Hin1I GRCGYC 2 cut(s) 377, 673
Hin1II CATG 8 cut(s) 274, 361, 428, 510, 871, 983, 1075, 1216
Hin6I GCGC 2 cut(s) 622, 673
HinP1I GCGC 2 cut(s) 622, 673
HincII GTYRAC 1 cut(s) 1179
HindII GTYRAC 1 cut(s) 1179
HindIII AAGCTT 1 cut(s) 1049
HinfI GANTC 8 cut(s) 241, 449, 635, 871, 895, 931, 959, 1315
HpaII CCGG 4 cut(s) 254, 493, 600, 639
HphI GGTGA 3 cut(s) 85, 147, 839
Hpy166II GTNNAC 2 cut(s) 1069, 1179
Hpy188I TCNGA 6 cut(s) 37, 240, 417, 864, 894, 1122
Hpy188III TCNNGA 9 cut(s) 50, 56, 71, 191, 254, 488, 868, 1112, 1213
Hpy8I GTNNAC 2 cut(s) 1069, 1179
HpyAV CCTTC 5 cut(s) 361, 574, 661, 771, 1093
HpyCH4IV ACGT 2 cut(s) 927, 1181
HpyCH4V TGCA 3 cut(s) 13, 748, 907
HpyF10VI GCNNNNNNNGC 8 cut(s) 223, 430, 543, 735, 751, 754, 976, 1031
HpyF3I CTNAG 4 cut(s) 306, 788, 1052, 1095
HpySE526I ACGT 2 cut(s) 927, 1181
Hsp92I GRCGYC 2 cut(s) 377, 673
Hsp92II CATG 8 cut(s) 274, 361, 428, 510, 871, 983, 1075, 1216
HspAI GCGC 2 cut(s) 622, 673
KasI GGCGCC 1 cut(s) 672
Kpn2I TCCGGA 1 cut(s) 253
KroI GCCGGC 1 cut(s) 599
KroNI GCCGGC 1 cut(s) 601
KspI CCGCGG 1 cut(s) 227
Kzo9I GATC 6 cut(s) 19, 46, 52, 67, 864, 1105
LmnI GCTCC 3 cut(s) 352, 567, 1204
Lsp1109I GCAGC 7 cut(s) 289, 329, 433, 549, 729, 732, 1206
LweI GCATC 2 cut(s) 586, 898
MaeI CTAG 5 cut(s) 407, 1005, 1011, 1026, 1239
MaeII ACGT 2 cut(s) 927, 1181
MaeIII GTNAC 1 cut(s) 373
MalI GATC 6 cut(s) 21, 48, 54, 69, 866, 1107
MbiI CCGCTC 1 cut(s) 433
MboI GATC 6 cut(s) 19, 46, 52, 67, 864, 1105
MhlI GDGCHC 1 cut(s) 357
MluCI AATT 6 cut(s) 5, 142, 325, 481, 985, 1208
Mly113I GGCGCC 1 cut(s) 673
MlyI GAGTC 1 cut(s) 443
MmeI TCCRAC 1 cut(s) 1115
MroI TCCGGA 1 cut(s) 253
MroNI GCCGGC 1 cut(s) 599
MroXI GAANNNNTTC 1 cut(s) 804
MseI TTAA 1 cut(s) 231
MspA1I CMGCKG 3 cut(s) 226, 445, 1040
MspI CCGG 4 cut(s) 254, 493, 600, 639
Mva1269I GAATGC 1 cut(s) 1082
MvnI CGCG 2 cut(s) 226, 624
MwoI GCNNNNNNNGC 8 cut(s) 223, 430, 543, 735, 751, 754, 976, 1031
NaeI GCCGGC 1 cut(s) 601
NarI GGCGCC 1 cut(s) 673
NcoI CCATGG 1 cut(s) 506
NdeII GATC 6 cut(s) 19, 46, 52, 67, 864, 1105
NgoMIV GCCGGC 1 cut(s) 599
NlaIII CATG 8 cut(s) 274, 361, 428, 510, 871, 983, 1075, 1216
NlaIV GGNNCC 4 cut(s) 151, 569, 674, 966
NmeAIII GCCGAG 3 cut(s) 405, 454, 676
NmuCI GTSAC 1 cut(s) 373
NspI RCATGY 3 cut(s) 361, 428, 983
NspV TTCGAA 1 cut(s) 815
PaeI GCATGC 2 cut(s) 428, 983
PagI TCATGA 2 cut(s) 867, 1212
PciI ACATGT 1 cut(s) 357
PctI GAATGC 1 cut(s) 1082
PdiI GCCGGC 1 cut(s) 601
PdmI GAANNNNTTC 1 cut(s) 804
PfeI GAWTC 7 cut(s) 241, 635, 871, 895, 931, 959, 1315
PleI GAGTC 1 cut(s) 443
PluTI GGCGCC 1 cut(s) 676
PmaCI CACGTG 1 cut(s) 928
PmlI CACGTG 1 cut(s) 928
PpsI GAGTC 1 cut(s) 443
Ppu21I YACGTR 1 cut(s) 928
PpuMI RGGWCCY 1 cut(s) 150
PscI ACATGT 1 cut(s) 357
Psp5II RGGWCCY 1 cut(s) 150
PspCI CACGTG 1 cut(s) 928
PspN4I GGNNCC 4 cut(s) 151, 569, 674, 966
PspPI GGNCC 1 cut(s) 150
PspPPI RGGWCCY 1 cut(s) 150
PstI CTGCAG 1 cut(s) 750
PvuII CAGCTG 1 cut(s) 1040
RsaI GTAC 8 cut(s) 164, 209, 316, 521, 839, 1009, 1237, 1246
RsaNI GTAC 8 cut(s) 163, 208, 315, 520, 838, 1008, 1236, 1245
SacII CCGCGG 1 cut(s) 227
SaqAI TTAA 1 cut(s) 231
Sau3AI GATC 6 cut(s) 19, 46, 52, 67, 864, 1105
Sau96I GGNCC 1 cut(s) 150
ScaI AGTACT 3 cut(s) 1009, 1237, 1246
SchI GAGTC 1 cut(s) 443
SduI GDGCHC 1 cut(s) 357
SfaNI GCATC 2 cut(s) 586, 898
SfcI CTRYAG 1 cut(s) 746
SfoI GGCGCC 1 cut(s) 674
Sfr303I CCGCGG 1 cut(s) 227
SfuI TTCGAA 1 cut(s) 815
SgrBI CCGCGG 1 cut(s) 227
SinI GGWCC 1 cut(s) 150
SmlI CTYRAG 1 cut(s) 1081
SmoI CTYRAG 1 cut(s) 1081
SpeI ACTAGT 3 cut(s) 1004, 1010, 1238
SphI GCATGC 2 cut(s) 428, 983
Sse9I AATT 6 cut(s) 5, 142, 325, 481, 985, 1208
SspDI GGCGCC 1 cut(s) 672
SspI AATATT 2 cut(s) 235, 881
SspMI CTAG 5 cut(s) 407, 1005, 1011, 1026, 1239
StyI CCWWGG 2 cut(s) 506, 947
TaiI ACGT 2 cut(s) 930, 1184
TaqI TCGA 2 cut(s) 815, 957
TasI AATT 6 cut(s) 5, 142, 325, 481, 985, 1208
TatI WGTACW 4 cut(s) 162, 1007, 1235, 1244
TauI GCSGC 4 cut(s) 226, 229, 757, 760
TfiI GAWTC 7 cut(s) 241, 635, 871, 895, 931, 959, 1315
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TseFI GTSAC 1 cut(s) 373
TseI GCWGC 7 cut(s) 302, 342, 421, 537, 742, 745, 1219
Tsp45I GTSAC 1 cut(s) 373
TspDTI ATGAA 9 cut(s) 102, 259, 646, 800, 818, 884, 887, 923, 1201
TspGWI ACGGA 2 cut(s) 825, 1191
VpaK11BI GGWCC 1 cut(s) 150
XapI RAATTY 2 cut(s) 5, 481
XceI RCATGY 3 cut(s) 361, 428, 983
XcmI CCANNNNNNNNNTGG 1 cut(s) 268
XmnI GAANNNNTTC 1 cut(s) 804
XspI CTAG 5 cut(s) 407, 1005, 1011, 1026, 1239
ZrmI AGTACT 3 cut(s) 1009, 1237, 1246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.