MD07G1182300.v1.1

Two-component response regulator-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0008778
Physical Location & Seq
Forward (+)
26269233 .. 26271837
2605 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1182300.v1.1.491

Sequence Viewer

Length: 780 bp
ATGGGGGAAGTTGGGATGAGTAGCAATAGTCCTCTTACCAAAGAGCGTGCGGAAGTAAATTGCCACATTCAAGATGAGAACAAGGAAACTAAGGATGGGGTTGTGGGAGAGGGGCGAGGGCTCCCAAAAAAAGATGATTTTCGGGTTAATGAGGACTTGCAAGGCGCACATGATGGGCGGGCAGAACAACAGCTCCAAGGGTGGGAGGAGTTCTTATCTGTCAGGTCTCTGAAGGTTCTTCTAGTGGAAAATGATGATGGAACTCGCAATATTCTTCGTGCTTTACTGCAAAATTGTGGCTACAAAGTAATAGAGGCAGATAATGGTATACGAGCTTGGGAAATCTTAGAAGATCTTACGACTCATGTTGATCTTATTTTGAGTGAGGTTGTCATGCCCAGTTTGTCAGGCATGGATCTCCTGACCAAGATAATGGACCACAAAACTCGCAAGAATATTCCTGTAATTATGATGTCGAGTCAAGATTCTATGAGTACAGTTATCAGGTGTTTATCGATGGGCGCAGTTGACTTTCTATTGAAGCCTATTCGAAAGAATGAACTTAAAAACCTCTGGCGGCATATTTGGAGGAAATGCCACAGGCAATCTAGTGGTAGTGGGAGTGCAAGTGATATCCGAACTCAAAAATCTACAGAGTCGTCAAGTTTTGAGGAGTCAGACAAGAACACTGGCAGTAATTGTGATGATAACATCGGCCTTGGAAACATTGGTCTGACTGATAGGGATGAAGGTGACAATGGAAGTGGCACTGAGGTATGA

Protein Analysis

260

Amino Acids

28.78

Weight (kDa)

5.1

Isoelectric Point (pI)

56.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Response_reg PF00072 79 - 190 8.8e-25 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 328
AciI CCGC 3 cut(s) 50, 178, 577
AclWI GGATC 1 cut(s) 423
AcuI CTGAAG 1 cut(s) 251
AfaI GTAC 1 cut(s) 496
AfiI CCNNNNNNNGG 1 cut(s) 202
AgsI TTSAA 2 cut(s) 71, 541
AloI GAACNNNNNNTCC 2 cut(s) 177, 209
AluBI AGCT 2 cut(s) 193, 335
AluI AGCT 2 cut(s) 193, 335
Alw26I GTCTC 1 cut(s) 231
AlwI GGATC 1 cut(s) 423
AoxI GGCC 1 cut(s) 715
AspLEI GCGC 2 cut(s) 167, 524
AspS9I GGNCC 1 cut(s) 436
AsuHPI GGTGA 1 cut(s) 764
AsuII TTCGAA 1 cut(s) 550
AvaII GGWCC 1 cut(s) 436
BanII GRGCYC 1 cut(s) 123
BccI CCATC 4 cut(s) 89, 167, 251, 511
BcoDI GTCTC 1 cut(s) 231
BfaI CTAG 2 cut(s) 242, 609
BfmI CTRYAG 1 cut(s) 651
BglII AGATCT 1 cut(s) 352
BisI GCNGC 1 cut(s) 578
BlsI GCNGC 1 cut(s) 579
Bme18I GGWCC 1 cut(s) 436
BmgT120I GGNCC 1 cut(s) 436
BmiI GGNNCC 1 cut(s) 122
BmrI ACTGGG 1 cut(s) 393
BmuI ACTGGG 1 cut(s) 393
Bpu14I TTCGAA 1 cut(s) 550
Bsa29I ATCGAT 1 cut(s) 515
BsaI GGTCTC 1 cut(s) 231
BsaJI CCNNGG 2 cut(s) 196, 718
BsaXI ACNNNNNCTCC 4 cut(s) 177, 207, 613, 643
Bsc4I CCNNNNNNNGG 1 cut(s) 202
Bse1I ACTGG 2 cut(s) 399, 694
BseCI ATCGAT 1 cut(s) 515
BseDI CCNNGG 2 cut(s) 196, 718
BseGI GGATG 3 cut(s) 21, 100, 751
BseLI CCNNNNNNNGG 1 cut(s) 202
BseMII CTCAG 1 cut(s) 762
BseNI ACTGG 2 cut(s) 399, 694
BseRI GAGGAG 2 cut(s) 221, 686
BshFI GGCC 1 cut(s) 717
BshVI ATCGAT 1 cut(s) 515
BslI CCNNNNNNNGG 1 cut(s) 202
BsmAI GTCTC 1 cut(s) 231
BsnI GGCC 1 cut(s) 717
Bso31I GGTCTC 1 cut(s) 231
Bsp119I TTCGAA 1 cut(s) 550
Bsp1286I GDGCHC 1 cut(s) 123
Bsp143I GATC 3 cut(s) 352, 370, 415
BspACI CCGC 3 cut(s) 50, 178, 577
BspANI GGCC 1 cut(s) 717
BspCNI CTCAG 1 cut(s) 763
BspDI ATCGAT 1 cut(s) 515
BspLI GGNNCC 1 cut(s) 122
BspPI GGATC 1 cut(s) 423
BspT104I TTCGAA 1 cut(s) 550
BspTNI GGTCTC 1 cut(s) 231
BsrI ACTGG 2 cut(s) 399, 694
BssECI CCNNGG 2 cut(s) 196, 718
BssMI GATC 3 cut(s) 352, 370, 415
BssNAI GTATAC 1 cut(s) 329
BssT1I CCWWGG 2 cut(s) 196, 718
Bst1107I GTATAC 1 cut(s) 329
Bst4CI ACNGT 1 cut(s) 499
BstBI TTCGAA 1 cut(s) 550
BstC8I GCNNGC 2 cut(s) 48, 180
BstDEI CTNAG 3 cut(s) 90, 346, 771
BstF5I GGATG 3 cut(s) 21, 100, 751
BstHHI GCGC 2 cut(s) 167, 524
BstKTI GATC 3 cut(s) 355, 373, 418
BstMAI GTCTC 1 cut(s) 231
BstMBI GATC 3 cut(s) 352, 370, 415
BstSFI CTRYAG 1 cut(s) 651
BstX2I RGATCY 2 cut(s) 352, 415
BstXI CCANNNNNNTGG 1 cut(s) 433
BstYI RGATCY 2 cut(s) 352, 415
BstZ17I GTATAC 1 cut(s) 329
Bsu15I ATCGAT 1 cut(s) 515
BsuRI GGCC 1 cut(s) 717
BsuTUI ATCGAT 1 cut(s) 515
BtsCI GGATG 3 cut(s) 21, 100, 751
BtsIMutI CAGTG 2 cut(s) 687, 768
Cac8I GCNNGC 2 cut(s) 48, 180
CfoI GCGC 2 cut(s) 167, 524
Cfr13I GGNCC 1 cut(s) 436
ClaI ATCGAT 1 cut(s) 515
Csp6I GTAC 1 cut(s) 495
CspCI CAANNNNNGTGG 2 cut(s) 745, 780
CviAII CATG 4 cut(s) 170, 365, 394, 412
CviJI RGCY 6 cut(s) 121, 193, 300, 335, 544, 717
CviKI_1 RGCY 6 cut(s) 121, 193, 300, 335, 544, 717
CviQI GTAC 1 cut(s) 495
DdeI CTNAG 3 cut(s) 90, 346, 771
DpnI GATC 3 cut(s) 354, 372, 417
DpnII GATC 3 cut(s) 352, 370, 415
Eco130I CCWWGG 2 cut(s) 196, 718
Eco24I GRGCYC 1 cut(s) 123
Eco31I GGTCTC 1 cut(s) 231
Eco32I GATATC 1 cut(s) 634
Eco47I GGWCC 1 cut(s) 436
Eco57I CTGAAG 1 cut(s) 251
EcoRV GATATC 1 cut(s) 634
EcoT14I CCWWGG 2 cut(s) 196, 718
EcoT38I GRGCYC 1 cut(s) 123
ErhI CCWWGG 2 cut(s) 196, 718
FaeI CATG 4 cut(s) 173, 368, 397, 415
FaiI YATR 9 cut(s) 171, 329, 366, 395, 413, 470, 491, 582, 778
FatI CATG 4 cut(s) 169, 364, 393, 411
FauI CCCGC 1 cut(s) 171
FblI GTMKAC 1 cut(s) 328
Fnu4HI GCNGC 1 cut(s) 578
FokI GGATG 3 cut(s) 28, 107, 758
FriOI GRGCYC 1 cut(s) 123
Fsp4HI GCNGC 1 cut(s) 578
FspBI CTAG 2 cut(s) 242, 609
GlaI GCGC 2 cut(s) 166, 523
GluI GCNGC 1 cut(s) 578
HaeIII GGCC 1 cut(s) 717
HhaI GCGC 2 cut(s) 167, 524
Hin1II CATG 4 cut(s) 173, 368, 397, 415
Hin6I GCGC 2 cut(s) 165, 522
HinP1I GCGC 2 cut(s) 165, 522
HincII GTYRAC 1 cut(s) 529
HindII GTYRAC 1 cut(s) 529
HinfI GANTC 5 cut(s) 361, 478, 485, 656, 674
HphI GGTGA 1 cut(s) 764
Hpy166II GTNNAC 2 cut(s) 329, 529
Hpy188I TCNGA 4 cut(s) 231, 638, 679, 735
Hpy188III TCNNGA 3 cut(s) 71, 421, 482
Hpy8I GTNNAC 2 cut(s) 329, 529
HpyAV CCTTC 2 cut(s) 226, 743
HpyCH4III ACNGT 1 cut(s) 499
HpyCH4V TGCA 3 cut(s) 160, 289, 626
HpyF3I CTNAG 3 cut(s) 90, 346, 771
Hsp92II CATG 4 cut(s) 173, 368, 397, 415
HspAI GCGC 2 cut(s) 165, 522
Kzo9I GATC 3 cut(s) 352, 370, 415
LmnI GCTCC 2 cut(s) 126, 198
LpnPI CCDG 9 cut(s) 208, 393, 412, 434, 474, 490, 559, 586, 675
MaeI CTAG 2 cut(s) 242, 609
MaeIII GTNAC 1 cut(s) 752
MalI GATC 3 cut(s) 354, 372, 417
MboI GATC 3 cut(s) 352, 370, 415
MboII GAAGA 3 cut(s) 230, 266, 362
MflI RGATCY 2 cut(s) 352, 415
MhlI GDGCHC 1 cut(s) 123
MluCI AATT 4 cut(s) 58, 292, 465, 697
MlyI GAGTC 4 cut(s) 355, 487, 665, 683
MseI TTAA 2 cut(s) 147, 564
NdeII GATC 3 cut(s) 352, 370, 415
NlaIII CATG 4 cut(s) 173, 368, 397, 415
NlaIV GGNNCC 1 cut(s) 122
NmuCI GTSAC 1 cut(s) 752
NspV TTCGAA 1 cut(s) 550
PfeI GAWTC 1 cut(s) 485
PkrI GCNGC 1 cut(s) 579
PleI GAGTC 4 cut(s) 355, 486, 664, 682
PpsI GAGTC 4 cut(s) 355, 486, 664, 682
PspN4I GGNNCC 1 cut(s) 122
PspPI GGNCC 1 cut(s) 436
PsuI RGATCY 2 cut(s) 352, 415
RsaI GTAC 1 cut(s) 496
RsaNI GTAC 1 cut(s) 495
SaqAI TTAA 2 cut(s) 147, 564
SatI GCNGC 1 cut(s) 578
Sau3AI GATC 3 cut(s) 352, 370, 415
Sau96I GGNCC 1 cut(s) 436
SchI GAGTC 4 cut(s) 355, 487, 665, 683
SduI GDGCHC 1 cut(s) 123
SetI ASST 9 cut(s) 195, 227, 237, 337, 390, 509, 573, 754, 777
SfcI CTRYAG 1 cut(s) 651
SfuI TTCGAA 1 cut(s) 550
SinI GGWCC 1 cut(s) 436
Sse9I AATT 4 cut(s) 58, 292, 465, 697
SsiI CCGC 3 cut(s) 50, 178, 577
SspI AATATT 2 cut(s) 271, 457
SspMI CTAG 2 cut(s) 242, 609
StyI CCWWGG 2 cut(s) 196, 718
TaaI ACNGT 1 cut(s) 499
TaqI TCGA 3 cut(s) 476, 515, 550
TasI AATT 4 cut(s) 58, 292, 465, 697
TatI WGTACW 1 cut(s) 494
TauI GCSGC 1 cut(s) 580
TfiI GAWTC 1 cut(s) 485
Tru1I TTAA 2 cut(s) 147, 564
Tru9I TTAA 2 cut(s) 147, 564
TscAI CASTG 2 cut(s) 694, 775
TseFI GTSAC 1 cut(s) 752
Tsp45I GTSAC 1 cut(s) 752
TspDTI ATGAA 2 cut(s) 573, 762
TspRI CASTG 2 cut(s) 694, 775
VpaK11BI GGWCC 1 cut(s) 436
XmiI GTMKAC 1 cut(s) 328
XspI CTAG 2 cut(s) 242, 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.