Rw1G028750

Two-component response regulator-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0008778
Physical Location & Seq
Forward (+)
56439250 .. 56444313
5064 bp
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UTR
Exon/CDS
Intron
Rw1G028750.1

Sequence Viewer

Length: 990 bp
ATGGGGAAAGTTCGGGTGAATATCAATGGTCCAGTAACCAATAAAGAGAGTGTGGATTTAAATTGCCATACTGAAGATGAGAACAAGGAGATTAGCGGTGCTGTTACAGGAGTCTTGGGAGAGGGCCAAGGGATCTTAAAGGAAGATGAAGTGCAGGTTACTGACAATCTGCAAGTCATAAATGATGCACAAACAGAAGCAGTCCAGGTTCAGGTCCTCAGTCAGTCTGGCCAGCAAAGTTCGGAACAACAGCTTCAAGACCCTTCTCTTTTCTGGGAAGGGTTCCTGCCTACCAAGTCTCTCAAGGTTTTGGTAGTGGAAGATGATGATTCAACTCGGCGTGTCATTTGTGCATTACTGCAGAAGTGTGGCTATGAAGTAACAGCTGTAGCTAGTGGATTGCAAGCTTGGAATATTTTAAAAGATCATAGTATTCATGTCGATCTTGTTTTAACTGAGGTTGTCATGCCTGGTCCATCAGGCATTGACCTCCTAAGCAAGATGATGAGCCACGAAACTTGCAAGGATATTCCTGTGATTATGATGTCATCTAATGATTCTTTGAGTATGGTGTTTAAGTGTCTATCAAAGGGTGCGGTTGACTTTTTAGTGAAGCCTATTCGAAAGAACGAGCTTAAAAATCTTTGGCAGCATGCTTGGAGGAAATGCCGGAGCTCCAGTGGTAGTGCAAGTGAAAATGGTGTCCAGACTCAAAAAACTACGTCGACAAGTGATGAAGAGTCAGACAAAAACACTGGCAGTAATCATGAGGATGACAATGAAATCATTGGGGTTAATGCTAGGGATGAAAGTGACAAAGGAAGTGGCACTGAGATTACTCAGGCTATTATTCGTTCAAGTCCTGAAGCATTTGGAAATGACTTGGTTCCTGCAGTTACAACAAGGGAGTGTGAAGGGCAGAATGAAGAGCTTGGTATGCATCTAAGTCTAATTATCTATAATTTTTTCCCTCTTTTTTGTTTGTGGTAA

Protein Analysis

329

Amino Acids

36.11

Weight (kDa)

4.68

Isoelectric Point (pI)

50.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Response_reg PF00072 103 - 214 3.2e-25 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 145
AccI GTMKAC 1 cut(s) 725
AciI CCGC 2 cut(s) 96, 596
AclWI GGATC 1 cut(s) 140
AcoI YGGCCR 1 cut(s) 229
AcuI CTGAAG 2 cut(s) 93, 885
AfiI CCNNNNNNNGG 1 cut(s) 211
AgsI TTSAA 3 cut(s) 257, 333, 858
AjnI CCWGG 2 cut(s) 204, 469
AluBI AGCT 7 cut(s) 253, 386, 392, 407, 634, 675, 931
AluI AGCT 7 cut(s) 253, 386, 392, 407, 634, 675, 931
Alw21I GWGCWC 1 cut(s) 677
Alw26I GTCTC 1 cut(s) 303
AlwI GGATC 1 cut(s) 140
AoxI GGCC 2 cut(s) 124, 229
ApeKI GCWGC 1 cut(s) 649
AspS9I GGNCC 4 cut(s) 29, 124, 214, 473
AsuHPI GGTGA 1 cut(s) 28
AsuII TTCGAA 1 cut(s) 622
AvaII GGWCC 3 cut(s) 29, 214, 473
BalI TGGCCA 1 cut(s) 231
BanII GRGCYC 1 cut(s) 677
Bbv12I GWGCWC 1 cut(s) 677
BbvI GCAGC 1 cut(s) 661
BccI CCATC 1 cut(s) 484
BciT130I CCWGG 2 cut(s) 206, 471
BcoDI GTCTC 1 cut(s) 303
BfaI CTAG 2 cut(s) 393, 801
BfmI CTRYAG 3 cut(s) 359, 387, 891
BfuAI ACCTGC 1 cut(s) 145
BisI GCNGC 1 cut(s) 650
BlsI GCNGC 1 cut(s) 651
Bme1390I CCNGG 2 cut(s) 206, 471
Bme18I GGWCC 3 cut(s) 29, 214, 473
BmgT120I GGNCC 4 cut(s) 29, 124, 214, 473
BmiI GGNNCC 2 cut(s) 284, 888
BmrFI CCNGG 2 cut(s) 206, 471
BmsI GCATC 2 cut(s) 175, 949
BpmI CTGGAG 1 cut(s) 661
Bpu10I CCTNAGC 1 cut(s) 494
Bpu14I TTCGAA 1 cut(s) 622
BpuEI CTTGAG 1 cut(s) 287
BsaJI CCNNGG 1 cut(s) 127
BsaXI ACNNNNNCTCC 2 cut(s) 664, 694
Bsc4I CCNNNNNNNGG 1 cut(s) 211
Bse1I ACTGG 3 cut(s) 32, 678, 760
BseBI CCWGG 2 cut(s) 206, 471
BseDI CCNNGG 1 cut(s) 127
BseGI GGATG 2 cut(s) 778, 811
BseLI CCNNNNNNNGG 1 cut(s) 211
BseMII CTCAG 4 cut(s) 232, 447, 822, 854
BseNI ACTGG 3 cut(s) 32, 678, 760
BseXI GCAGC 1 cut(s) 661
BsgI GTGCAG 1 cut(s) 173
BshFI GGCC 2 cut(s) 126, 231
BsiHKAI GWGCWC 1 cut(s) 677
BsiSI CCGG 1 cut(s) 670
BslI CCNNNNNNNGG 1 cut(s) 211
BsmAI GTCTC 1 cut(s) 303
BsnI GGCC 2 cut(s) 126, 231
Bsp119I TTCGAA 1 cut(s) 622
Bsp1286I GDGCHC 1 cut(s) 677
Bsp143I GATC 3 cut(s) 132, 424, 442
BspACI CCGC 2 cut(s) 96, 596
BspANI GGCC 2 cut(s) 126, 231
BspCNI CTCAG 4 cut(s) 231, 448, 823, 853
BspHI TCATGA 1 cut(s) 766
BspLI GGNNCC 2 cut(s) 284, 888
BspMAI CTGCAG 2 cut(s) 363, 895
BspMI ACCTGC 1 cut(s) 145
BspPI GGATC 1 cut(s) 140
BspQI GCTCTTC 1 cut(s) 921
BspT104I TTCGAA 1 cut(s) 622
BsrI ACTGG 3 cut(s) 32, 678, 760
BssECI CCNNGG 1 cut(s) 127
BssMI GATC 3 cut(s) 132, 424, 442
BssT1I CCWWGG 1 cut(s) 127
Bst2UI CCWGG 2 cut(s) 206, 471
Bst6I CTCTTC 2 cut(s) 732, 921
BstBI TTCGAA 1 cut(s) 622
BstC8I GCNNGC 3 cut(s) 233, 405, 654
BstDEI CTNAG 6 cut(s) 218, 456, 494, 831, 840, 944
BstF5I GGATG 2 cut(s) 778, 811
BstKTI GATC 3 cut(s) 135, 427, 445
BstMAI GTCTC 1 cut(s) 303
BstMBI GATC 3 cut(s) 132, 424, 442
BstMWI GCNNNNNNNGC 1 cut(s) 937
BstNI CCWGG 2 cut(s) 206, 471
BstNSI RCATGY 1 cut(s) 656
BstSCI CCNGG 2 cut(s) 204, 469
BstSFI CTRYAG 3 cut(s) 359, 387, 891
BstV1I GCAGC 1 cut(s) 661
BstX2I RGATCY 1 cut(s) 132
BstYI RGATCY 1 cut(s) 132
BsuRI GGCC 2 cut(s) 126, 231
BtsCI GGATG 2 cut(s) 778, 811
BtsIMutI CAGTG 3 cut(s) 685, 753, 828
BveI ACCTGC 1 cut(s) 145
Cac8I GCNNGC 3 cut(s) 233, 405, 654
CciI TCATGA 1 cut(s) 766
Cfr13I GGNCC 4 cut(s) 29, 124, 214, 473
CspCI CAANNNNNGTGG 4 cut(s) 500, 535, 805, 840
CviAII CATG 4 cut(s) 437, 466, 653, 767
DdeI CTNAG 6 cut(s) 218, 456, 494, 831, 840, 944
DpnI GATC 3 cut(s) 134, 426, 444
DpnII GATC 3 cut(s) 132, 424, 442
DraI TTTAAA 2 cut(s) 60, 420
EaeI YGGCCR 1 cut(s) 229
Eam1104I CTCTTC 2 cut(s) 732, 921
EarI CTCTTC 2 cut(s) 732, 921
Ecl136II GAGCTC 1 cut(s) 675
Eco130I CCWWGG 1 cut(s) 127
Eco24I GRGCYC 1 cut(s) 677
Eco47I GGWCC 3 cut(s) 29, 214, 473
Eco53kI GAGCTC 1 cut(s) 675
Eco57I CTGAAG 2 cut(s) 93, 885
EcoICRI GAGCTC 1 cut(s) 675
EcoO109I RGGNCCY 1 cut(s) 214
EcoRII CCWGG 2 cut(s) 204, 469
EcoT14I CCWWGG 1 cut(s) 127
EcoT22I ATGCAT 1 cut(s) 942
EcoT38I GRGCYC 1 cut(s) 677
ErhI CCWWGG 1 cut(s) 127
FaeI CATG 4 cut(s) 440, 469, 656, 770
FatI CATG 4 cut(s) 436, 465, 652, 766
FblI GTMKAC 1 cut(s) 725
Fnu4HI GCNGC 1 cut(s) 650
FokI GGATG 2 cut(s) 785, 818
FriOI GRGCYC 1 cut(s) 677
Fsp4HI GCNGC 1 cut(s) 650
FspBI CTAG 2 cut(s) 393, 801
GluI GCNGC 1 cut(s) 650
GsuI CTGGAG 1 cut(s) 661
HaeIII GGCC 2 cut(s) 126, 231
HapII CCGG 1 cut(s) 670
Hin1II CATG 4 cut(s) 440, 469, 656, 770
HincII GTYRAC 2 cut(s) 601, 726
HindII GTYRAC 2 cut(s) 601, 726
HindIII AAGCTT 1 cut(s) 405
HinfI GANTC 5 cut(s) 111, 329, 557, 709, 740
HpaII CCGG 1 cut(s) 670
HphI GGTGA 1 cut(s) 28
Hpy166II GTNNAC 2 cut(s) 601, 726
Hpy188I TCNGA 2 cut(s) 244, 745
Hpy188III TCNNGA 4 cut(s) 257, 706, 767, 863
Hpy8I GTNNAC 2 cut(s) 601, 726
Hpy99I CGWCG 1 cut(s) 727
HpyAV CCTTC 3 cut(s) 272, 273, 908
HpyCH4IV ACGT 1 cut(s) 722
HpyF10VI GCNNNNNNNGC 1 cut(s) 937
HpyF3I CTNAG 6 cut(s) 218, 456, 494, 831, 840, 944
HpySE526I ACGT 1 cut(s) 722
Hsp92II CATG 4 cut(s) 440, 469, 656, 770
Kzo9I GATC 3 cut(s) 132, 424, 442
LguI GCTCTTC 1 cut(s) 921
LmnI GCTCC 2 cut(s) 672, 680
Lsp1109I GCAGC 1 cut(s) 661
LweI GCATC 2 cut(s) 175, 949
MaeI CTAG 2 cut(s) 393, 801
MaeII ACGT 1 cut(s) 722
MaeIII GTNAC 6 cut(s) 34, 103, 157, 379, 812, 895
MalI GATC 3 cut(s) 134, 426, 444
MboI GATC 3 cut(s) 132, 424, 442
MboII GAAGA 5 cut(s) 86, 155, 332, 749, 938
MflI RGATCY 1 cut(s) 132
MhlI GDGCHC 1 cut(s) 677
MlsI TGGCCA 1 cut(s) 231
MluCI AATT 3 cut(s) 61, 951, 961
MluNI TGGCCA 1 cut(s) 231
MlyI GAGTC 3 cut(s) 120, 703, 749
MnlI CCTC 7 cut(s) 115, 227, 451, 500, 654, 763, 981
Mox20I TGGCCA 1 cut(s) 231
Mph1103I ATGCAT 1 cut(s) 942
MscI TGGCCA 1 cut(s) 231
MseI TTAA 7 cut(s) 59, 137, 419, 452, 576, 636, 795
MslI CAYNNNNRTG 1 cut(s) 771
Msp20I TGGCCA 1 cut(s) 231
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 1 cut(s) 670
MspR9I CCNGG 2 cut(s) 206, 471
MvaI CCWGG 2 cut(s) 206, 471
MwoI GCNNNNNNNGC 1 cut(s) 937
NdeII GATC 3 cut(s) 132, 424, 442
NlaIII CATG 4 cut(s) 440, 469, 656, 770
NlaIV GGNNCC 2 cut(s) 284, 888
NmeAIII GCCGAG 1 cut(s) 316
NmuCI GTSAC 1 cut(s) 812
NsiI ATGCAT 1 cut(s) 942
NspI RCATGY 1 cut(s) 656
NspV TTCGAA 1 cut(s) 622
PaeI GCATGC 1 cut(s) 656
PagI TCATGA 1 cut(s) 766
PciSI GCTCTTC 1 cut(s) 921
PfeI GAWTC 2 cut(s) 329, 557
PkrI GCNGC 1 cut(s) 651
PleI GAGTC 3 cut(s) 119, 703, 748
PpsI GAGTC 3 cut(s) 119, 703, 748
PpuMI RGGWCCY 1 cut(s) 214
Psp124BI GAGCTC 1 cut(s) 677
Psp5II RGGWCCY 1 cut(s) 214
Psp6I CCWGG 2 cut(s) 204, 469
PspGI CCWGG 2 cut(s) 204, 469
PspN4I GGNNCC 2 cut(s) 284, 888
PspPI GGNCC 4 cut(s) 29, 124, 214, 473
PspPPI RGGWCCY 1 cut(s) 214
PstI CTGCAG 2 cut(s) 363, 895
PsuI RGATCY 1 cut(s) 132
PvuII CAGCTG 1 cut(s) 386
RseI CAYNNNNRTG 1 cut(s) 771
SacI GAGCTC 1 cut(s) 677
SalI GTCGAC 1 cut(s) 724
SapI GCTCTTC 1 cut(s) 921
SaqAI TTAA 7 cut(s) 59, 137, 419, 452, 576, 636, 795
SatI GCNGC 1 cut(s) 650
Sau3AI GATC 3 cut(s) 132, 424, 442
Sau96I GGNCC 4 cut(s) 29, 124, 214, 473
SchI GAGTC 3 cut(s) 120, 703, 749
ScrFI CCNGG 2 cut(s) 206, 471
SduI GDGCHC 1 cut(s) 677
SfaNI GCATC 2 cut(s) 175, 949
SfcI CTRYAG 3 cut(s) 359, 387, 891
SfuI TTCGAA 1 cut(s) 622
SinI GGWCC 3 cut(s) 29, 214, 473
SmiI ATTTAAAT 1 cut(s) 60
SmiMI CAYNNNNRTG 1 cut(s) 771
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
SphI GCATGC 1 cut(s) 656
Sse9I AATT 3 cut(s) 61, 951, 961
SsiI CCGC 2 cut(s) 96, 596
SspI AATATT 1 cut(s) 415
SspMI CTAG 2 cut(s) 393, 801
SstI GAGCTC 1 cut(s) 677
StyD4I CCNGG 2 cut(s) 204, 469
StyI CCWWGG 1 cut(s) 127
SwaI ATTTAAAT 1 cut(s) 60
TaiI ACGT 1 cut(s) 725
TaqI TCGA 3 cut(s) 441, 622, 725
TasI AATT 3 cut(s) 61, 951, 961
TfiI GAWTC 2 cut(s) 329, 557
Tru1I TTAA 7 cut(s) 59, 137, 419, 452, 576, 636, 795
Tru9I TTAA 7 cut(s) 59, 137, 419, 452, 576, 636, 795
TscAI CASTG 3 cut(s) 685, 760, 835
TseFI GTSAC 1 cut(s) 812
TseI GCWGC 1 cut(s) 649
Tsp45I GTSAC 1 cut(s) 812
TspDTI ATGAA 7 cut(s) 162, 390, 425, 750, 795, 822, 939
TspRI CASTG 3 cut(s) 685, 760, 835
VpaK11BI GGWCC 3 cut(s) 29, 214, 473
XceI RCATGY 1 cut(s) 656
XmiI GTMKAC 1 cut(s) 725
XspI CTAG 2 cut(s) 393, 801
Zsp2I ATGCAT 1 cut(s) 942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.