MD07G1186600.v1.1

membrane-associated kinase regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007268
Physical Location & Seq
Forward (+)
26769497 .. 26770585
1089 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1186600.v1.1.491

Sequence Viewer

Length: 1089 bp
ATGGCTGTAGACCACCTATCCTGTGACCATGCAGACGACGACTACATCGACATGGATGTCGGTTCGTACTCTGCCTTCCTCAACCATTCCATGAGCTCTCCTCCACACCCCAGAGAGTTTGAGTTTCAAATGTCATCCACTTCTTTGGAGAGAGATCCCCCCACAACTTCTCCAGCTGATGAGCTCTTTTACAAAGGGAAGCTCCTTCCCCTCCACCTCCCCCCGCGCTTACAAATGGTCGAGAAACTACTGCAGAACTCCTATTCTAGTTTTGATCACCAAAAAGATATGTTTGAAGAATTCTATAGCACTCCATTGGCCACCACTGCCCCAACTCCAACCACAACCAGCACCCCATTTGAGTCCTGCAATATATCGCCTTCGGAGTCTTGCCAGGTTAGTAGGGAGCTGAACCCCGAAGAGTATATTTTGGAGTACTCAACTGAAGTGAGCGGCTTCATAAATGAAAACCCGAAAAAGTCTTGGACCAAAAAGTTCAGGCAGTCATTGCTTAGTTCAAAGCTGAAGTCTTCCCGGGCTTACCTCAAGTCTCTGTTTGGCAAATACAGTTGCTCCAATGAGTCCAGTGCAGCTGCTGCCAGGCATGCACATGGAGGAATGGTTTTCAAATCTGAGGATTTAAGTAAATATATGAAGACATCCAAGAACCCATTTGGACACACTCAGAGGGAGAAATACCGAATGTCTGCCTCTGGCGCGAGGAGCTTCAGCAAAGACAAGATCATTGAGGATGGTGCTGCTCTCCACAGGAGATCATTCTCACTGGCCATCAAACGGAATTCAACGAAGATCTCGACATCCTCTTCATCATCTTCGGGCTCTTCATCTTCTTCTTCATCATCTTCAAATCATTCAAGTGGGACTCAGGAGCTGCAGTTTCTGAAGAGATGCAACAGTGCAAGTTCCGAAATTGAGAGTTCAATTCAGGGGGCAATTGCACATTGCAAGCAGTCGCAGCAACCTTTCCGTTCAAGAAAGACAGTAAGTGAAGTTGGGTTCTACTCGTTGTCAGCTTCCAGGATAGCAGTTTGCGAGGATCAAGAAAGACCGGAGCTTTGCAGGGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0009506 GO:0030054 GO:0055044
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

363

Amino Acids

40.23

Weight (kDa)

8.04

Isoelectric Point (pI)

67.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 56
AccBSI CCGCTC 1 cut(s) 453
AccI GTMKAC 1 cut(s) 9
AccII CGCG 2 cut(s) 226, 719
AciI CCGC 2 cut(s) 224, 453
AclWI GGATC 2 cut(s) 149, 1065
AcoI YGGCCR 2 cut(s) 318, 786
AcsI RAATTY 2 cut(s) 299, 799
AcuI CTGAAG 4 cut(s) 465, 545, 712, 923
AfaI GTAC 2 cut(s) 68, 437
AfiI CCNNNNNNNGG 1 cut(s) 795
AgsI TTSAA 9 cut(s) 128, 296, 519, 628, 804, 867, 876, 942, 993
AjnI CCWGG 3 cut(s) 393, 599, 1037
Alw21I GWGCWC 2 cut(s) 98, 186
Alw26I GTCTC 1 cut(s) 555
AlwI GGATC 2 cut(s) 149, 1065
AlwNI CAGNNNCTG 4 cut(s) 596, 892, 901, 1086
Ama87I CYCGRG 1 cut(s) 534
AoxI GGCC 2 cut(s) 318, 786
ApeKI GCWGC 6 cut(s) 590, 593, 596, 758, 892, 976
ApoI RAATTY 2 cut(s) 299, 799
ArsI GACNNNNNNTTYG 3 cut(s) 512, 544, 1059
AspLEI GCGC 2 cut(s) 228, 719
AspS9I GGNCC 1 cut(s) 486
AsuC2I CCSGG 2 cut(s) 535, 536
AsuHPI GGTGA 1 cut(s) 269
AvaI CYCGRG 1 cut(s) 534
AvaII GGWCC 1 cut(s) 486
BalI TGGCCA 2 cut(s) 320, 788
BanII GRGCYC 3 cut(s) 98, 186, 842
BarI GAAGNNNNNNTAC 2 cut(s) 59, 91
BbsI GAAGAC 2 cut(s) 522, 662
Bbv12I GWGCWC 2 cut(s) 98, 186
BbvI GCAGC 6 cut(s) 580, 583, 602, 745, 879, 988
BccI CCATC 2 cut(s) 746, 797
BciT130I CCWGG 3 cut(s) 395, 601, 1039
BclI TGATCA 1 cut(s) 274
BcnI CCSGG 2 cut(s) 535, 536
BcoDI GTCTC 1 cut(s) 555
BfaI CTAG 1 cut(s) 267
BfmI CTRYAG 4 cut(s) 6, 251, 304, 893
BglII AGATCT 1 cut(s) 810
BisI GCNGC 7 cut(s) 454, 591, 594, 597, 759, 893, 977
BlsI GCNGC 7 cut(s) 455, 592, 595, 598, 760, 894, 978
BmcAI AGTACT 1 cut(s) 437
Bme1390I CCNGG 5 cut(s) 395, 535, 536, 601, 1039
Bme18I GGWCC 1 cut(s) 486
BmeT110I CYCGRG 1 cut(s) 534
BmgT120I GGNCC 1 cut(s) 486
BmrFI CCNGG 5 cut(s) 395, 535, 536, 601, 1039
BmsI GCATC 1 cut(s) 899
BpiI GAAGAC 2 cut(s) 522, 662
BplI GAGNNNNNCTC 2 cut(s) 85, 117
BpmI CTGGAG 1 cut(s) 156
BpuEI CTTGAG 1 cut(s) 530
BpuMI CCSGG 2 cut(s) 535, 536
BsaJI CCNNGG 1 cut(s) 534
BsaWI WCCGGW 1 cut(s) 1069
BsaXI ACNNNNNCTCC 8 cut(s) 154, 184, 557, 587, 606, 636, 881, 911
Bsc4I CCNNNNNNNGG 1 cut(s) 795
Bse1I ACTGG 2 cut(s) 585, 789
Bse3DI GCAATG 2 cut(s) 506, 961
BseBI CCWGG 3 cut(s) 395, 601, 1039
BseDI CCNNGG 1 cut(s) 534
BseGI GGATG 5 cut(s) 61, 134, 659, 757, 818
BseLI CCNNNNNNNGG 1 cut(s) 795
BseMI GCAATG 2 cut(s) 506, 961
BseMII CTCAG 3 cut(s) 624, 698, 899
BseNI ACTGG 2 cut(s) 585, 789
BseRI GAGGAG 2 cut(s) 90, 736
BseXI GCAGC 6 cut(s) 580, 583, 602, 745, 879, 988
BsgI GTGCAG 1 cut(s) 609
Bsh1236I CGCG 2 cut(s) 226, 719
BshFI GGCC 2 cut(s) 320, 788
BsiHKAI GWGCWC 2 cut(s) 98, 186
BsiHKCI CYCGRG 1 cut(s) 534
BsiSI CCGG 2 cut(s) 535, 1070
BslFI GGGAC 1 cut(s) 895
BslI CCNNNNNNNGG 1 cut(s) 795
BsmAI GTCTC 1 cut(s) 555
BsmFI GGGAC 1 cut(s) 895
BsnI GGCC 2 cut(s) 320, 788
BsoBI CYCGRG 1 cut(s) 534
Bsp1286I GDGCHC 3 cut(s) 98, 186, 842
Bsp143I GATC 6 cut(s) 154, 274, 741, 773, 810, 1057
BspACI CCGC 2 cut(s) 224, 453
BspANI GGCC 2 cut(s) 320, 788
BspCNI CTCAG 3 cut(s) 625, 697, 898
BspFNI CGCG 2 cut(s) 226, 719
BspMAI CTGCAG 2 cut(s) 255, 897
BspPI GGATC 2 cut(s) 149, 1065
BspQI GCTCTTC 1 cut(s) 847
BsrBI CCGCTC 1 cut(s) 453
BsrDI GCAATG 2 cut(s) 506, 961
BsrI ACTGG 2 cut(s) 585, 789
BssECI CCNNGG 1 cut(s) 534
BssMI GATC 6 cut(s) 154, 274, 741, 773, 810, 1057
Bst2UI CCWGG 3 cut(s) 395, 601, 1039
Bst4CI ACNGT 3 cut(s) 569, 917, 1003
Bst6I CTCTTC 4 cut(s) 414, 829, 847, 899
BstAPI GCANNNNNTGC 2 cut(s) 508, 596
BstC8I GCNNGC 2 cut(s) 606, 968
BstDEI CTNAG 4 cut(s) 512, 633, 684, 885
BstF5I GGATG 5 cut(s) 61, 134, 659, 757, 818
BstFNI CGCG 2 cut(s) 226, 719
BstHHI GCGC 2 cut(s) 228, 719
BstKTI GATC 6 cut(s) 157, 277, 744, 776, 813, 1060
BstMAI GTCTC 1 cut(s) 555
BstMBI GATC 6 cut(s) 154, 274, 741, 773, 810, 1057
BstMWI GCNNNNNNNGC 7 cut(s) 326, 508, 596, 605, 716, 723, 976
BstNI CCWGG 3 cut(s) 395, 601, 1039
BstNSI RCATGY 1 cut(s) 608
BstSCI CCNGG 5 cut(s) 393, 533, 534, 599, 1037
BstSFI CTRYAG 4 cut(s) 6, 251, 304, 893
BstUI CGCG 2 cut(s) 226, 719
BstV1I GCAGC 6 cut(s) 580, 583, 602, 745, 879, 988
BstV2I GAAGAC 2 cut(s) 522, 662
BstX2I RGATCY 2 cut(s) 154, 810
BstXI CCANNNNNNTGG 1 cut(s) 145
BstYI RGATCY 2 cut(s) 154, 810
BsuRI GGCC 2 cut(s) 320, 788
BtsCI GGATG 5 cut(s) 61, 134, 659, 757, 818
BtsI GCAGTG 1 cut(s) 324
BtsIMutI CAGTG 4 cut(s) 324, 592, 782, 922
Cac8I GCNNGC 2 cut(s) 606, 968
CaiI CAGNNNCTG 4 cut(s) 596, 892, 901, 1086
CfoI GCGC 2 cut(s) 228, 719
Cfr13I GGNCC 1 cut(s) 486
Cfr9I CCCGGG 1 cut(s) 534
Csp6I GTAC 2 cut(s) 67, 436
CviAII CATG 5 cut(s) 29, 52, 91, 605, 611
CviQI GTAC 2 cut(s) 67, 436
DdeI CTNAG 4 cut(s) 512, 633, 684, 885
DpnI GATC 6 cut(s) 156, 276, 743, 775, 812, 1059
DpnII GATC 6 cut(s) 154, 274, 741, 773, 810, 1057
DrdI GACNNNNNNGTC 1 cut(s) 56
DseDI GACNNNNNNGTC 1 cut(s) 56
EaeI YGGCCR 2 cut(s) 318, 786
Eam1104I CTCTTC 4 cut(s) 414, 829, 847, 899
EarI CTCTTC 4 cut(s) 414, 829, 847, 899
Ecl136II GAGCTC 2 cut(s) 96, 184
Eco24I GRGCYC 3 cut(s) 98, 186, 842
Eco47I GGWCC 1 cut(s) 486
Eco53kI GAGCTC 2 cut(s) 96, 184
Eco57I CTGAAG 4 cut(s) 465, 545, 712, 923
Eco88I CYCGRG 1 cut(s) 534
EcoICRI GAGCTC 2 cut(s) 96, 184
EcoRI GAATTC 2 cut(s) 299, 799
EcoRII CCWGG 3 cut(s) 393, 599, 1037
EcoT38I GRGCYC 3 cut(s) 98, 186, 842
FaeI CATG 5 cut(s) 32, 55, 94, 608, 614
FaqI GGGAC 1 cut(s) 895
FatI CATG 5 cut(s) 28, 51, 90, 604, 610
FauI CCCGC 1 cut(s) 231
FbaI TGATCA 1 cut(s) 274
FblI GTMKAC 1 cut(s) 9
Fnu4HI GCNGC 7 cut(s) 454, 591, 594, 597, 759, 893, 977
FokI GGATG 5 cut(s) 68, 121, 646, 764, 805
FriOI GRGCYC 3 cut(s) 98, 186, 842
Fsp4HI GCNGC 7 cut(s) 454, 591, 594, 597, 759, 893, 977
FspBI CTAG 1 cut(s) 267
GlaI GCGC 2 cut(s) 227, 718
GluI GCNGC 7 cut(s) 454, 591, 594, 597, 759, 893, 977
GsuI CTGGAG 1 cut(s) 156
HaeIII GGCC 2 cut(s) 320, 788
HapII CCGG 2 cut(s) 535, 1070
HhaI GCGC 2 cut(s) 228, 719
Hin1II CATG 5 cut(s) 32, 55, 94, 608, 614
Hin6I GCGC 2 cut(s) 226, 717
HinP1I GCGC 2 cut(s) 226, 717
HinfI GANTC 4 cut(s) 362, 386, 581, 883
HpaII CCGG 2 cut(s) 535, 1070
HphI GGTGA 1 cut(s) 269
Hpy166II GTNNAC 1 cut(s) 10
Hpy188I TCNGA 5 cut(s) 385, 634, 687, 903, 928
Hpy188III TCNNGA 5 cut(s) 241, 814, 887, 993, 1061
Hpy8I GTNNAC 1 cut(s) 10
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 3 cut(s) 85, 215, 390
HpyCH4III ACNGT 3 cut(s) 569, 917, 1003
HpyF10VI GCNNNNNNNGC 7 cut(s) 326, 508, 596, 605, 716, 723, 976
HpyF3I CTNAG 4 cut(s) 512, 633, 684, 885
Hsp92II CATG 5 cut(s) 32, 55, 94, 608, 614
HspAI GCGC 2 cut(s) 226, 717
Ksp22I TGATCA 1 cut(s) 274
Kzo9I GATC 6 cut(s) 154, 274, 741, 773, 810, 1057
LguI GCTCTTC 1 cut(s) 847
LmnI GCTCC 6 cut(s) 207, 406, 578, 723, 889, 1072
Lsp1109I GCAGC 6 cut(s) 580, 583, 602, 745, 879, 988
LweI GCATC 1 cut(s) 899
MaeI CTAG 1 cut(s) 267
MaeIII GTNAC 1 cut(s) 23
MalI GATC 6 cut(s) 156, 276, 743, 775, 812, 1059
MbiI CCGCTC 1 cut(s) 453
MboI GATC 6 cut(s) 154, 274, 741, 773, 810, 1057
MfeI CAATTG 1 cut(s) 954
MflI RGATCY 2 cut(s) 154, 810
MhlI GDGCHC 3 cut(s) 98, 186, 842
MlsI TGGCCA 2 cut(s) 320, 788
MluCI AATT 5 cut(s) 299, 799, 930, 942, 954
MluNI TGGCCA 2 cut(s) 320, 788
MlyI GAGTC 4 cut(s) 371, 395, 590, 877
MmeI TCCRAC 1 cut(s) 362
Mox20I TGGCCA 2 cut(s) 320, 788
MscI TGGCCA 2 cut(s) 320, 788
MseI TTAA 1 cut(s) 641
MslI CAYNNNNRTG 3 cut(s) 50, 609, 876
Msp20I TGGCCA 2 cut(s) 320, 788
MspA1I CMGCKG 2 cut(s) 176, 593
MspI CCGG 2 cut(s) 535, 1070
MspR9I CCNGG 5 cut(s) 395, 535, 536, 601, 1039
MunI CAATTG 1 cut(s) 954
MvaI CCWGG 3 cut(s) 395, 601, 1039
MvnI CGCG 2 cut(s) 226, 719
MwoI GCNNNNNNNGC 7 cut(s) 326, 508, 596, 605, 716, 723, 976
NciI CCSGG 2 cut(s) 535, 536
NdeII GATC 6 cut(s) 154, 274, 741, 773, 810, 1057
NlaIII CATG 5 cut(s) 32, 55, 94, 608, 614
NmuCI GTSAC 1 cut(s) 23
NspI RCATGY 1 cut(s) 608
PaeI GCATGC 1 cut(s) 608
PciSI GCTCTTC 1 cut(s) 847
PcsI WCGNNNNNNNCGW 2 cut(s) 45, 812
PfoI TCCNGGA 1 cut(s) 1037
PkrI GCNGC 7 cut(s) 455, 592, 595, 598, 760, 894, 978
PleI GAGTC 4 cut(s) 370, 394, 589, 877
PpsI GAGTC 4 cut(s) 370, 394, 589, 877
Psp124BI GAGCTC 2 cut(s) 98, 186
Psp6I CCWGG 3 cut(s) 393, 599, 1037
PspGI CCWGG 3 cut(s) 393, 599, 1037
PspPI GGNCC 1 cut(s) 486
PstI CTGCAG 2 cut(s) 255, 897
PstNI CAGNNNCTG 4 cut(s) 596, 892, 901, 1086
PsuI RGATCY 2 cut(s) 154, 810
PvuII CAGCTG 2 cut(s) 176, 593
RsaI GTAC 2 cut(s) 68, 437
RsaNI GTAC 2 cut(s) 67, 436
RseI CAYNNNNRTG 3 cut(s) 50, 609, 876
SacI GAGCTC 2 cut(s) 98, 186
SapI GCTCTTC 1 cut(s) 847
SaqAI TTAA 1 cut(s) 641
SatI GCNGC 7 cut(s) 454, 591, 594, 597, 759, 893, 977
Sau3AI GATC 6 cut(s) 154, 274, 741, 773, 810, 1057
Sau96I GGNCC 1 cut(s) 486
ScaI AGTACT 1 cut(s) 437
SchI GAGTC 4 cut(s) 371, 395, 590, 877
ScrFI CCNGG 5 cut(s) 395, 535, 536, 601, 1039
SduI GDGCHC 3 cut(s) 98, 186, 842
SfaNI GCATC 1 cut(s) 899
SfcI CTRYAG 4 cut(s) 6, 251, 304, 893
SinI GGWCC 1 cut(s) 486
SmaI CCCGGG 1 cut(s) 536
SmiMI CAYNNNNRTG 3 cut(s) 50, 609, 876
SmlI CTYRAG 1 cut(s) 545
SmoI CTYRAG 1 cut(s) 545
SphI GCATGC 1 cut(s) 608
Sse9I AATT 5 cut(s) 299, 799, 930, 942, 954
SsiI CCGC 2 cut(s) 224, 453
SspMI CTAG 1 cut(s) 267
SstI GAGCTC 2 cut(s) 98, 186
StyD4I CCNGG 5 cut(s) 393, 533, 534, 599, 1037
TaaI ACNGT 3 cut(s) 569, 917, 1003
TaqI TCGA 3 cut(s) 48, 240, 815
TasI AATT 5 cut(s) 299, 799, 930, 942, 954
TatI WGTACW 1 cut(s) 435
TauI GCSGC 1 cut(s) 456
Tru1I TTAA 1 cut(s) 641
Tru9I TTAA 1 cut(s) 641
TscAI CASTG 4 cut(s) 331, 592, 789, 922
TseFI GTSAC 1 cut(s) 23
TseI GCWGC 6 cut(s) 590, 593, 596, 758, 892, 976
Tsp45I GTSAC 1 cut(s) 23
TspDTI ATGAA 6 cut(s) 448, 480, 668, 816, 834, 846
TspGWI ACGGA 2 cut(s) 811, 977
TspMI CCCGGG 1 cut(s) 534
TspRI CASTG 4 cut(s) 331, 592, 789, 922
VpaK11BI GGWCC 1 cut(s) 486
XapI RAATTY 2 cut(s) 299, 799
XceI RCATGY 1 cut(s) 608
XmaI CCCGGG 1 cut(s) 534
XmiI GTMKAC 1 cut(s) 9
XspI CTAG 1 cut(s) 267
ZrmI AGTACT 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.