Rh2DG256000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0007268
Physical Location & Seq
Forward (+)
27182441 .. 27193897
11457 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG256000.1

Sequence Viewer

Length: 282 bp
ATGGGTGATGGTGTTCTAACTCTTAAGTCCAGAACTCCAGATAATTTCTCCATGATGAGGCCTTCCTGGCCTGGGTCCGGCCCAACGAACAGGAGACCTCCGCCTGAGCCAATGCGAAGGTCTATTCGCGCTTCAGCCCCGATCGTTCACCGGCGGGATGCTCTACATGCAGAACAACCTCGACATGGCCGCCGCGTCCCTCACCAACAGCTCCGCGACCTCGATCTGCTGCTCAGATCTGGCGTGCTCCTTCGTCTCACCCCTCGCCGCTCCAAACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

93

Amino Acids

10.68

Weight (kDa)

12.0

Isoelectric Point (pI)

78.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 270
AccII CGCG 3 cut(s) 129, 195, 216
AciI CCGC 6 cut(s) 101, 154, 190, 193, 214, 268
AcoI YGGCCR 1 cut(s) 187
AcuI CTGAAG 1 cut(s) 117
AfiI CCNNNNNNNGG 4 cut(s) 57, 72, 77, 185
AflII CTTAAG 1 cut(s) 23
AjnI CCWGG 2 cut(s) 65, 70
AluBI AGCT 1 cut(s) 211
AluI AGCT 1 cut(s) 211
Alw21I GWGCWC 1 cut(s) 249
Alw26I GTCTC 2 cut(s) 88, 260
AoxI GGCC 4 cut(s) 59, 68, 79, 187
ApeKI GCWGC 1 cut(s) 229
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 2 cut(s) 75, 80
AsuHPI GGTGA 4 cut(s) 17, 140, 194, 250
AvaII GGWCC 1 cut(s) 75
Bbv12I GWGCWC 1 cut(s) 249
BbvI GCAGC 1 cut(s) 216
BccI CCATC 1 cut(s) 2
BciT130I CCWGG 2 cut(s) 67, 72
BcoDI GTCTC 2 cut(s) 88, 260
BfrI CTTAAG 1 cut(s) 23
BglI GCCNNNNNGGC 1 cut(s) 67
BglII AGATCT 1 cut(s) 236
BisI GCNGC 4 cut(s) 190, 193, 230, 268
BlsI GCNGC 4 cut(s) 191, 194, 231, 269
Bme1390I CCNGG 2 cut(s) 67, 72
Bme18I GGWCC 1 cut(s) 75
BmgT120I GGNCC 2 cut(s) 75, 80
BmiI GGNNCC 1 cut(s) 76
BmrFI CCNGG 2 cut(s) 67, 72
BmsI GCATC 1 cut(s) 148
BpmI CTGGAG 1 cut(s) 21
Bpu10I CCTNAGC 1 cut(s) 105
BsaI GGTCTC 1 cut(s) 88
BsaJI CCNNGG 1 cut(s) 71
Bsc4I CCNNNNNNNGG 4 cut(s) 57, 72, 77, 185
Bse118I RCCGGY 1 cut(s) 150
BseBI CCWGG 2 cut(s) 67, 72
BseDI CCNNGG 1 cut(s) 71
BseGI GGATG 1 cut(s) 163
BseLI CCNNNNNNNGG 4 cut(s) 57, 72, 77, 185
BseMII CTCAG 2 cut(s) 96, 247
BseXI GCAGC 1 cut(s) 216
Bsh1236I CGCG 3 cut(s) 129, 195, 216
Bsh1285I CGRYCG 1 cut(s) 144
BshFI GGCC 4 cut(s) 61, 70, 81, 189
BsiEI CGRYCG 1 cut(s) 144
BsiHKAI GWGCWC 1 cut(s) 249
BsiSI CCGG 2 cut(s) 78, 151
BslFI GGGAC 1 cut(s) 182
BslI CCNNNNNNNGG 4 cut(s) 57, 72, 77, 185
BsmAI GTCTC 2 cut(s) 88, 260
BsmBI CGTCTC 1 cut(s) 260
BsmFI GGGAC 1 cut(s) 182
BsnI GGCC 4 cut(s) 61, 70, 81, 189
Bso31I GGTCTC 1 cut(s) 88
Bsp1286I GDGCHC 1 cut(s) 249
Bsp143I GATC 3 cut(s) 141, 223, 236
BspACI CCGC 6 cut(s) 101, 154, 190, 193, 214, 268
BspANI GGCC 4 cut(s) 61, 70, 81, 189
BspCNI CTCAG 2 cut(s) 97, 246
BspFNI CGCG 3 cut(s) 129, 195, 216
BspLI GGNNCC 1 cut(s) 76
BspTI CTTAAG 1 cut(s) 23
BspTNI GGTCTC 1 cut(s) 88
BsrBI CCGCTC 1 cut(s) 270
BsrFI RCCGGY 1 cut(s) 150
BssAI RCCGGY 1 cut(s) 150
BssECI CCNNGG 1 cut(s) 71
BssMI GATC 3 cut(s) 141, 223, 236
Bst2UI CCWGG 2 cut(s) 67, 72
BstAFI CTTAAG 1 cut(s) 23
BstC8I GCNNGC 1 cut(s) 245
BstDEI CTNAG 2 cut(s) 105, 233
BstF5I GGATG 1 cut(s) 163
BstFNI CGCG 3 cut(s) 129, 195, 216
BstHHI GCGC 1 cut(s) 131
BstKTI GATC 3 cut(s) 144, 226, 239
BstMAI GTCTC 2 cut(s) 88, 260
BstMBI GATC 3 cut(s) 141, 223, 236
BstMCI CGRYCG 1 cut(s) 144
BstMWI GCNNNNNNNGC 2 cut(s) 67, 167
BstNI CCWGG 2 cut(s) 67, 72
BstNSI RCATGY 1 cut(s) 170
BstSCI CCNGG 2 cut(s) 65, 70
BstUI CGCG 3 cut(s) 129, 195, 216
BstV1I GCAGC 1 cut(s) 216
BstX2I RGATCY 1 cut(s) 236
BstYI RGATCY 1 cut(s) 236
BsuRI GGCC 4 cut(s) 61, 70, 81, 189
BtsCI GGATG 1 cut(s) 163
Cac8I GCNNGC 1 cut(s) 245
CfoI GCGC 1 cut(s) 131
Cfr10I RCCGGY 1 cut(s) 150
Cfr13I GGNCC 2 cut(s) 75, 80
CseI GACGC 1 cut(s) 184
CviAII CATG 3 cut(s) 52, 167, 185
CviJI RGCY 7 cut(s) 61, 70, 81, 109, 137, 189, 211
CviKI_1 RGCY 7 cut(s) 61, 70, 81, 109, 137, 189, 211
DdeI CTNAG 2 cut(s) 105, 233
DpnI GATC 3 cut(s) 143, 225, 238
DpnII GATC 3 cut(s) 141, 223, 236
EaeI YGGCCR 1 cut(s) 187
EciI GGCGGA 1 cut(s) 90
Eco147I AGGCCT 1 cut(s) 61
Eco31I GGTCTC 1 cut(s) 88
Eco47I GGWCC 1 cut(s) 75
Eco57I CTGAAG 1 cut(s) 117
EcoRII CCWGG 2 cut(s) 65, 70
Esp3I CGTCTC 1 cut(s) 260
FaeI CATG 3 cut(s) 55, 170, 188
FaiI YATR 3 cut(s) 53, 168, 186
FaqI GGGAC 1 cut(s) 182
FatI CATG 3 cut(s) 51, 166, 184
FauI CCCGC 1 cut(s) 147
Fnu4HI GCNGC 4 cut(s) 190, 193, 230, 268
FokI GGATG 1 cut(s) 170
Fsp4HI GCNGC 4 cut(s) 190, 193, 230, 268
GlaI GCGC 1 cut(s) 130
GluI GCNGC 4 cut(s) 190, 193, 230, 268
GsuI CTGGAG 1 cut(s) 21
HaeIII GGCC 4 cut(s) 61, 70, 81, 189
HapII CCGG 2 cut(s) 78, 151
HgaI GACGC 1 cut(s) 184
HhaI GCGC 1 cut(s) 131
Hin1II CATG 3 cut(s) 55, 170, 188
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HpaII CCGG 2 cut(s) 78, 151
HphI GGTGA 4 cut(s) 17, 140, 194, 250
Hpy166II GTNNAC 1 cut(s) 148
Hpy188I TCNGA 1 cut(s) 236
Hpy188III TCNNGA 2 cut(s) 30, 38
Hpy8I GTNNAC 1 cut(s) 148
HpyAV CCTTC 3 cut(s) 72, 111, 260
HpyCH4V TGCA 1 cut(s) 170
HpyF10VI GCNNNNNNNGC 2 cut(s) 67, 167
HpyF3I CTNAG 2 cut(s) 105, 233
Hsp92II CATG 3 cut(s) 55, 170, 188
HspAI GCGC 1 cut(s) 129
Kzo9I GATC 3 cut(s) 141, 223, 236
LmnI GCTCC 3 cut(s) 216, 252, 275
Lsp1109I GCAGC 1 cut(s) 216
LweI GCATC 1 cut(s) 148
MalI GATC 3 cut(s) 143, 225, 238
MbiI CCGCTC 1 cut(s) 270
MboI GATC 3 cut(s) 141, 223, 236
MflI RGATCY 1 cut(s) 236
MhlI GDGCHC 1 cut(s) 249
MluCI AATT 1 cut(s) 43
MnlI CCTC 6 cut(s) 51, 108, 189, 210, 230, 273
MseI TTAA 1 cut(s) 24
MspCI CTTAAG 1 cut(s) 23
MspI CCGG 2 cut(s) 78, 151
MspR9I CCNGG 2 cut(s) 67, 72
MvaI CCWGG 2 cut(s) 67, 72
MvnI CGCG 3 cut(s) 129, 195, 216
MwoI GCNNNNNNNGC 2 cut(s) 67, 167
NdeII GATC 3 cut(s) 141, 223, 236
NlaIII CATG 3 cut(s) 55, 170, 188
NlaIV GGNNCC 1 cut(s) 76
NspI RCATGY 1 cut(s) 170
PceI AGGCCT 1 cut(s) 61
PkrI GCNGC 4 cut(s) 191, 194, 231, 269
Ple19I CGATCG 1 cut(s) 144
Psp6I CCWGG 2 cut(s) 65, 70
PspGI CCWGG 2 cut(s) 65, 70
PspN4I GGNNCC 1 cut(s) 76
PspPI GGNCC 2 cut(s) 75, 80
PsuI RGATCY 1 cut(s) 236
PvuI CGATCG 1 cut(s) 144
SaqAI TTAA 1 cut(s) 24
SatI GCNGC 4 cut(s) 190, 193, 230, 268
Sau3AI GATC 3 cut(s) 141, 223, 236
Sau96I GGNCC 2 cut(s) 75, 80
ScrFI CCNGG 2 cut(s) 67, 72
SduI GDGCHC 1 cut(s) 249
SetI ASST 5 cut(s) 100, 122, 181, 213, 222
SfaNI GCATC 1 cut(s) 148
SfiI GGCCNNNNNGGCC 1 cut(s) 67
SgrAI CRCCGGYG 1 cut(s) 150
SinI GGWCC 1 cut(s) 75
SmlI CTYRAG 1 cut(s) 23
SmoI CTYRAG 1 cut(s) 23
Sse9I AATT 1 cut(s) 43
SseBI AGGCCT 1 cut(s) 61
SsiI CCGC 6 cut(s) 101, 154, 190, 193, 214, 268
StuI AGGCCT 1 cut(s) 61
StyD4I CCNGG 2 cut(s) 65, 70
TaqI TCGA 2 cut(s) 181, 222
TasI AATT 1 cut(s) 43
TauI GCSGC 3 cut(s) 192, 195, 270
Tru1I TTAA 1 cut(s) 24
Tru9I TTAA 1 cut(s) 24
TseI GCWGC 1 cut(s) 229
Vha464I CTTAAG 1 cut(s) 23
VpaK11BI GGWCC 1 cut(s) 75
XceI RCATGY 1 cut(s) 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.