Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0011483

Physical Location & Seq

Genomic Coordinates Forward (+)

32604735 .. 32609399

Gene Structure 4665 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1259900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2052 bp

ATGCCTTCGATGGACGTGTCCGATTATCTACTCGCGGGAACCACTCCGAAGGACAAGAAGGAGAAGAAGAAGAAATTGAAAAGCGAAACCCTAGACGACGCCGCCGACTCGCCCACTACTGAGAAGAAGTCGAAGAAGGAGTCTAAGCACAAAAAGCGGAAGGCTTCGGATGACGAGGACAGGAGCGAGACGAGCTCCGAGATGGGCGAGCCCGTGAATGTGAAGCCGCCGAAGAGCGAAGAGAAGAGCAAGAAGAAGTCGAAGAAGGCGAAGGTGGAGCCGGAGGAGGAGGAGGAGAAGCCCAAAGAGGATCCTAATGCGGTGTCAAAGTTTCGGATTTCGGCGCCGTTGAGGGCCAAATTGAAGGAGAATGGGATCGAATCCTTGTTCCCTATTCAGGCCATGACATTCGATACCATTTTGGATGGATTGGATTTGGTCGGTCGGGCTCGAACTGGACAGGGTAAAACTCTAGCTTTTGTGTTACCCGTTTTGGAGTCCTTAACAAATGGGCCTGCAAAAGCATTTAGAAAGACTGGTTATGGTAGGCCTCCAACTGTTATCGTGCTTTTACCCACTCGGGAACTTGCCAAACAGGTTTTCGCCGATTTTGAAGCTTATGGTGGTGCTGTGGGGCTAGCAGCATGTTGTGTATATGGTGGATCTCCTTACGGGGCTCAAGAAAGTAAGTTAAGGAGAGGGGTGGATATTGTTATTGGAACGCCTGGCCGTATAAAGGATCATATAGAGAGGGGCAATATCGACTTGAGTACCTTGAAAGTTAGGATCCTTGACGAAGCTGATGAAATGCTGAGGATGGGCTTTGTTGACGATGTTGAACTCATTCTAGGCAAGGTGAAGGATGTAAGTAAAGTTCAAACGCTACTTTTTAGTGCAACCTTGCCAAGTTGGGTGAAAGGCATTTCTTCTAGGTTTCTCAAACCTGATAAGAAAACTGTAGATCTTGTTGGTAATGAAAAAATGAAGGCCAGCACCAATGTTAGGCATATTGTTCTTCCTTGCTCCAGTTCAGCAAGATCACAACTTATTCCTGATATCATACGTTGTTATAGTAGTGGAGGACGCACAATCATTTTTACCGAGACAAAGGAAGCTGCTTCTGGACTTGCTGAGGCATTGCCTGGAGCCCGAGCTTTGCACGGGGACATACAGCAGGGCCAACGTGAGGTCACACTTGCTGGTTTCAGATCTGGCAAGTTTTTGACATTAGTTGCCACAAATGTGGCAGCGCGTGGACTGGATATCAATGATGTTCAATTAATTATCCAGTGTGAACCCCCACGTGATGTAGAAGACTATATCCATCGATCTGGACGCACAGGGAGAGCAGGCAATTCCGGAGTTGCTGTCATGCTTTATGATCCAAGAAGATCAAATTTTTCAAAAATCGAGAGGGAGTCCGGGGTGAAATTTGAACACATTTCTGCTCCTCAGCCACTTGATATAGCTAAAGCAGTAGGTCAAGATGCAGCAGAAATGATAACCAAAATTTCTGATAGTGTAATTCCTGCTTTCAAGTCTGTTGCCGAGGAGCTACTTAACACCTCTGGCCTATCTGCAGTAGATCTACTGGCAAAAGCACTTGCTAAAGCTGCTGGCTACACCGAGATAAAGAAGAGATCACTTCTTAGCTCTATGGAGAACCATGTGACAGTACTTCTTGAGGCTGGAAAGCCCGTTTATTCACCGTCTTTTGCTTTTGGGGTCTTGAGGAGATTCTTGCCCGAGGAGAAGGTTGAGTCAGTGAAGGGTATGGCACTAACTGCCGATGGAAATGGTGCAGTTTTTGATGTGGCAGCTGAAGATTTGGATTTGTTTCTTGCTGGTCAGGAAAATGCAGCTAATGTGAGCATAACGGTGTTGAAATCACTGCCTGATTTACAAGAAAAAGAATCAAGGAATACAAGATTTGGCGGTGGTGGAAGGTTTGGCCGTGGTGGTGGTGGAGGTTTTCGAAATGATAGGTATTCCAACAACCGATCCGGTGGTGGTGGTGGTGGTGGTGGGAGAGGTCGCGGCAACAGATGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017151 GO:0019899 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko01110 map01110

Pfam Domains

Protein Families

DEAD GUCT Helicase_C

Protein Analysis

684

Amino Acids

73.76

Weight (kDa)

9.24

Isoelectric Point (pI)

42.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	131 - 306	3.8e-44	DEAD/DEAH box helicase
Helicase_C	PF00271	349 - 451	9.2e-26	Helicase conserved C-terminal domain
DD_DDX21-DDX50	PF26142	485 - 537	9.6e-20	DDX21/DDX50 dimerisation domain
GUCT	PF08152	540 - 635	4.2e-30	GUCT (NUC152) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011483)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G62190
fragaria_vesca	FvH4_7g28410
malus_domestica	MD01G1187800.v1.1 MD07G1259900.v1.1 MD07G1260300.v1.1
prunus_persica	Prupe.2G284200_v2.0.a1 Prupe.2G284200_v2.0.a1 Prupe.2G284200_v2.0.a1
pyrus_communis	pycom07g23330
rosa_chinensis	RchiOBHm_Chr1g0375371
rosa_laevigata	RLG00000026667
rosa_multiflora	Rmu_sc0000554.1_g000041 Rmu_ssc0000106.1_g000003
rosa_roxburghii	Rroxscaffold_4G00282810
rosa_rugosa	Rorug01G0390800
rosa_samantha	Rh1AG401100 Rh1BG363200 Rh1CG376500 Rh1DG394000
rosa_wichuraiana	Rw1G035610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	343
AccII	CGCG	3 cut(s)	35, 1252, 2037
AccIII	TCCGGA	1 cut(s)	1358
AciI	CCGC	7 cut(s)	35, 102, 157, 227, 320, 1935, 2037
AclWI	GGATC	9 cut(s)	305, 318, 383, 670, 747, 781, 794, 1376, 1995
AcoI	YGGCCR	2 cut(s)	727, 1951
AcsI	RAATTY	3 cut(s)	1396, 1430, 1509
AcuI	CTGAAG	1 cut(s)	1842
AcvI	CACGTG	1 cut(s)	1304
AcyI	GRCGYC	2 cut(s)	99, 344
AfaI	GTAC	2 cut(s)	772, 1677
AfiI	CCNNNNNNNGG	4 cut(s)	397, 736, 1002, 1186
AflIII	ACRYGT	1 cut(s)	15
AjiI	CACGTC	1 cut(s)	16
AjnI	CCWGG	2 cut(s)	724, 1141
AjuI	GAANNNNNNNTTGG	2 cut(s)	584, 616
AleI	CACNNNNGTG	1 cut(s)	1241
Alw21I	GWGCWC	1 cut(s)	197
Alw26I	GTCTC	2 cut(s)	182, 1097
AlwI	GGATC	9 cut(s)	305, 318, 383, 670, 747, 781, 794, 1376, 1995
Ama87I	CYCGRG	3 cut(s)	579, 1149, 1745
Aor13HI	TCCGGA	1 cut(s)	1358
AoxI	GGCC	9 cut(s)	354, 399, 512, 548, 727, 987, 1177, 1570, 1951
ApeKI	GCWGC	7 cut(s)	641, 1115, 1247, 1490, 1613, 1817, 1859
ApoI	RAATTY	3 cut(s)	1396, 1430, 1509
ArsI	GACNNNNNNTTYG	2 cut(s)	125, 157
AseI	ATTAAT	1 cut(s)	1280
Asp700I	GAANNNNTTC	1 cut(s)	843
AspLEI	GCGC	2 cut(s)	346, 1252
AspS9I	GGNCC	3 cut(s)	354, 512, 1177
AsuC2I	CCSGG	1 cut(s)	1423
AsuHPI	GGTGA	4 cut(s)	868, 925, 1438, 1698
AsuII	TTCGAA	1 cut(s)	1975
AsuNHI	GCTAGC	1 cut(s)	637
AvaI	CYCGRG	3 cut(s)	579, 1149, 1745
BaeI	ACNNNNGTAYC	2 cut(s)	754, 787
BamHI	GGATCC	2 cut(s)	310, 786
BanI	GGYRCC	1 cut(s)	343
BanII	GRGCYC	5 cut(s)	197, 213, 451, 679, 1150
BbrPI	CACGTG	1 cut(s)	1304
BbsI	GAAGAC	1 cut(s)	1320
Bbv12I	GWGCWC	1 cut(s)	197
BbvCI	CCTCAGC	3 cut(s)	812, 1131, 1452
BbvI	GCAGC	7 cut(s)	653, 1102, 1259, 1502, 1600, 1829, 1871
BccI	CCATC	7 cut(s)	4, 196, 419, 811, 1332, 1784, 2040
BceAI	ACGGC	3 cut(s)	331, 714, 1938
BciT130I	CCWGG	2 cut(s)	726, 1143
BcnI	CCSGG	1 cut(s)	1423
BcoDI	GTCTC	2 cut(s)	182, 1097
BfaI	CTAG	5 cut(s)	92, 473, 638, 848, 930
BfmI	CTRYAG	2 cut(s)	957, 1578
BfoI	RGCGCY	1 cut(s)	347
BglII	AGATCT	3 cut(s)	961, 1208, 1585
BmcAI	AGTACT	1 cut(s)	1677
Bme1390I	CCNGG	3 cut(s)	726, 1143, 1423
BmeT110I	CYCGRG	3 cut(s)	579, 1149, 1745
BmgBI	CACGTC	1 cut(s)	16
BmgT120I	GGNCC	3 cut(s)	354, 512, 1177
BmiI	GGNNCC	6 cut(s)	40, 279, 312, 345, 788, 1147
BmrFI	CCNGG	3 cut(s)	726, 1143, 1423
BmsI	GCATC	1 cut(s)	1477
BmtI	GCTAGC	1 cut(s)	641
BpiI	GAAGAC	1 cut(s)	1320
BplI	GAGNNNNNCTC	2 cut(s)	179, 211
BpmI	CTGGAG	2 cut(s)	1009, 1164
Bpu10I	CCTNAGC	3 cut(s)	812, 1131, 1452
Bpu14I	TTCGAA	1 cut(s)	1975
BpuEI	CTTGAG	4 cut(s)	663, 787, 1703, 1750
BpuMI	CCSGG	1 cut(s)	1423
Bsa29I	ATCGAT	1 cut(s)	1327
BsaAI	YACGTR	1 cut(s)	1304
BsaHI	GRCGYC	2 cut(s)	99, 344
BsaJI	CCNNGG	4 cut(s)	1422, 1548, 1746, 1954
BsaWI	WCCGGW	2 cut(s)	1358, 2003
BsaXI	ACNNNNNCTCC	2 cut(s)	1353, 1383
Bsc4I	CCNNNNNNNGG	4 cut(s)	397, 736, 1002, 1186
Bse1I	ACTGG	6 cut(s)	460, 541, 1026, 1263, 1288, 1596
Bse3DI	GCAATG	1 cut(s)	1136
BseAI	TCCGGA	1 cut(s)	1358
BseBI	CCWGG	2 cut(s)	726, 1143
BseCI	ATCGAT	1 cut(s)	1327
BseDI	CCNNGG	4 cut(s)	1422, 1548, 1746, 1954
BseGI	GGATG	4 cut(s)	175, 430, 822, 868
BseLI	CCNNNNNNNGG	4 cut(s)	397, 736, 1002, 1186
BseMI	GCAATG	1 cut(s)	1136
BseMII	CTCAG	4 cut(s)	111, 803, 1122, 1466
BseNI	ACTGG	6 cut(s)	460, 541, 1026, 1263, 1288, 1596
BseRI	GAGGAG	8 cut(s)	299, 302, 305, 308, 1440, 1565, 1747, 1763
BseXI	GCAGC	7 cut(s)	653, 1102, 1259, 1502, 1600, 1829, 1871
BsgI	GTGCAG	1 cut(s)	1821
Bsh1236I	CGCG	3 cut(s)	35, 1252, 2037
Bsh1285I	CGRYCG	1 cut(s)	445
BshFI	GGCC	9 cut(s)	356, 401, 514, 550, 729, 989, 1179, 1572, 1953
BshNI	GGYRCC	1 cut(s)	343
BshVI	ATCGAT	1 cut(s)	1327
BsiEI	CGRYCG	1 cut(s)	445
BsiHKAI	GWGCWC	1 cut(s)	197
BsiHKCI	CYCGRG	3 cut(s)	579, 1149, 1745
BsiSI	CCGG	4 cut(s)	281, 1359, 1422, 2004
BslFI	GGGAC	1 cut(s)	1178
BslI	CCNNNNNNNGG	4 cut(s)	397, 736, 1002, 1186
BsmAI	GTCTC	2 cut(s)	182, 1097
BsmBI	CGTCTC	1 cut(s)	182
BsmFI	GGGAC	1 cut(s)	1178
BsnI	GGCC	9 cut(s)	356, 401, 514, 550, 729, 989, 1179, 1572, 1953
BsoBI	CYCGRG	3 cut(s)	579, 1149, 1745
Bsp119I	TTCGAA	1 cut(s)	1975
Bsp1286I	GDGCHC	5 cut(s)	197, 213, 451, 679, 1150
Bsp13I	TCCGGA	1 cut(s)	1358
BspACI	CCGC	7 cut(s)	35, 102, 157, 227, 320, 1935, 2037
BspANI	GGCC	9 cut(s)	356, 401, 514, 550, 729, 989, 1179, 1572, 1953
BspCNI	CTCAG	4 cut(s)	112, 804, 1123, 1465
BspDI	ATCGAT	1 cut(s)	1327
BspEI	TCCGGA	1 cut(s)	1358
BspFNI	CGCG	3 cut(s)	35, 1252, 2037
BspLI	GGNNCC	6 cut(s)	40, 279, 312, 345, 788, 1147
BspMAI	CTGCAG	1 cut(s)	1582
BspOI	GCTAGC	1 cut(s)	641
BspPI	GGATC	9 cut(s)	305, 318, 383, 670, 747, 781, 794, 1376, 1995
BspQI	GCTCTTC	2 cut(s)	227, 239
BspT104I	TTCGAA	1 cut(s)	1975
BspT107I	GGYRCC	1 cut(s)	343
BsrDI	GCAATG	1 cut(s)	1136
BsrI	ACTGG	6 cut(s)	460, 541, 1026, 1263, 1288, 1596
BssECI	CCNNGG	4 cut(s)	1422, 1548, 1746, 1954
BssNI	GRCGYC	2 cut(s)	99, 344
Bst2UI	CCWGG	2 cut(s)	726, 1143
Bst4CI	ACNGT	5 cut(s)	559, 958, 1675, 1710, 1879
Bst6I	CTCTTC	4 cut(s)	227, 234, 239, 1631
BstACI	GRCGYC	2 cut(s)	99, 344
BstAPI	GCANNNNNTGC	1 cut(s)	1784
BstBAI	YACGTR	1 cut(s)	1304
BstBI	TTCGAA	1 cut(s)	1975
BstC8I	GCNNGC	6 cut(s)	209, 516, 639, 991, 1351, 1618
BstDEI	CTNAG	6 cut(s)	120, 144, 812, 1131, 1452, 1649
BstDSI	CCRYGG	1 cut(s)	1954
BstF5I	GGATG	4 cut(s)	175, 430, 822, 868
BstFNI	CGCG	3 cut(s)	35, 1252, 2037
BstH2I	RGCGCY	1 cut(s)	347
BstHHI	GCGC	2 cut(s)	346, 1252
BstMAI	GTCTC	2 cut(s)	182, 1097
BstMCI	CGRYCG	1 cut(s)	445
BstMWI	GCNNNNNNNGC	4 cut(s)	154, 192, 1613, 1784
BstNI	CCWGG	2 cut(s)	726, 1143
BstNSI	RCATGY	1 cut(s)	648
BstSCI	CCNGG	3 cut(s)	724, 1141, 1421
BstSFI	CTRYAG	2 cut(s)	957, 1578
BstUI	CGCG	3 cut(s)	35, 1252, 2037
BstV1I	GCAGC	7 cut(s)	653, 1102, 1259, 1502, 1600, 1829, 1871
BstV2I	GAAGAC	1 cut(s)	1320
BstX2I	RGATCY	6 cut(s)	310, 662, 786, 961, 1208, 1585
BstXI	CCANNNNNNTGG	2 cut(s)	1243, 1331
BstYI	RGATCY	6 cut(s)	310, 662, 786, 961, 1208, 1585
Bsu15I	ATCGAT	1 cut(s)	1327
BsuRI	GGCC	9 cut(s)	356, 401, 514, 550, 729, 989, 1179, 1572, 1953
BsuTUI	ATCGAT	1 cut(s)	1327
BtgI	CCRYGG	1 cut(s)	1954
BtrI	CACGTC	1 cut(s)	16
BtsCI	GGATG	4 cut(s)	175, 430, 822, 868
BtsI	GCAGTG	1 cut(s)	1889
BtsIMutI	CAGTG	3 cut(s)	1295, 1770, 1889
Cac8I	GCNNGC	6 cut(s)	209, 516, 639, 991, 1351, 1618
CfoI	GCGC	2 cut(s)	346, 1252
Cfr13I	GGNCC	3 cut(s)	354, 512, 1177
ClaI	ATCGAT	1 cut(s)	1327
CseI	GACGC	3 cut(s)	107, 1092, 1344
Csp6I	GTAC	2 cut(s)	771, 1676
CviAII	CATG	4 cut(s)	403, 645, 1372, 1667
CviQI	GTAC	2 cut(s)	771, 1676
DdeI	CTNAG	6 cut(s)	120, 144, 812, 1131, 1452, 1649
DinI	GGCGCC	1 cut(s)	345
EaeI	YGGCCR	2 cut(s)	727, 1951
Eam1104I	CTCTTC	4 cut(s)	227, 234, 239, 1631
EarI	CTCTTC	4 cut(s)	227, 234, 239, 1631
Ecl136II	GAGCTC	1 cut(s)	195
Eco147I	AGGCCT	1 cut(s)	550
Eco24I	GRGCYC	5 cut(s)	197, 213, 451, 679, 1150
Eco32I	GATATC	2 cut(s)	1057, 1264
Eco53kI	GAGCTC	1 cut(s)	195
Eco57I	CTGAAG	1 cut(s)	1842
Eco72I	CACGTG	1 cut(s)	1304
Eco88I	CYCGRG	3 cut(s)	579, 1149, 1745
EcoICRI	GAGCTC	1 cut(s)	195
EcoRII	CCWGG	2 cut(s)	724, 1141
EcoRV	GATATC	2 cut(s)	1057, 1264
EcoT38I	GRGCYC	5 cut(s)	197, 213, 451, 679, 1150
EgeI	GGCGCC	1 cut(s)	345
EheI	GGCGCC	1 cut(s)	345
Esp3I	CGTCTC	1 cut(s)	182
FaeI	CATG	4 cut(s)	406, 648, 1375, 1670
FaqI	GGGAC	1 cut(s)	1178
FatI	CATG	4 cut(s)	402, 644, 1371, 1666
FauI	CCCGC	1 cut(s)	28
FokI	GGATG	4 cut(s)	182, 437, 829, 875
FriOI	GRGCYC	5 cut(s)	197, 213, 451, 679, 1150
FspBI	CTAG	5 cut(s)	92, 473, 638, 848, 930
GlaI	GCGC	2 cut(s)	345, 1251
GsuI	CTGGAG	2 cut(s)	1009, 1164
HaeII	RGCGCY	1 cut(s)	347
HaeIII	GGCC	9 cut(s)	356, 401, 514, 550, 729, 989, 1179, 1572, 1953
HapII	CCGG	4 cut(s)	281, 1359, 1422, 2004
HgaI	GACGC	3 cut(s)	107, 1092, 1344
HhaI	GCGC	2 cut(s)	346, 1252
Hin1I	GRCGYC	2 cut(s)	99, 344
Hin1II	CATG	4 cut(s)	406, 648, 1375, 1670
Hin6I	GCGC	2 cut(s)	344, 1250
HinP1I	GCGC	2 cut(s)	344, 1250
HincII	GTYRAC	1 cut(s)	829
HindII	GTYRAC	1 cut(s)	829
HindIII	AAGCTT	1 cut(s)	615
HinfI	GANTC	8 cut(s)	107, 140, 380, 497, 1418, 1737, 1760, 1913
HpaII	CCGG	4 cut(s)	281, 1359, 1422, 2004
HphI	GGTGA	4 cut(s)	868, 925, 1438, 1698
Hpy166II	GTNNAC	3 cut(s)	829, 1256, 1295
Hpy188I	TCNGA	7 cut(s)	22, 48, 169, 199, 336, 1208, 1516
Hpy8I	GTNNAC	3 cut(s)	829, 1256, 1295
Hpy99I	CGWCG	1 cut(s)	101
HpyCH4III	ACNGT	5 cut(s)	559, 958, 1675, 1710, 1879
HpyCH4IV	ACGT	4 cut(s)	15, 1063, 1183, 1303
HpyCH4V	TGCA	7 cut(s)	518, 896, 1159, 1490, 1580, 1802, 1859
HpyF10VI	GCNNNNNNNGC	4 cut(s)	154, 192, 1613, 1784
HpyF3I	CTNAG	6 cut(s)	120, 144, 812, 1131, 1452, 1649
HpySE526I	ACGT	4 cut(s)	15, 1063, 1183, 1303
Hsp92I	GRCGYC	2 cut(s)	99, 344
Hsp92II	CATG	4 cut(s)	406, 648, 1375, 1670
HspAI	GCGC	2 cut(s)	344, 1250
KasI	GGCGCC	1 cut(s)	343
Kpn2I	TCCGGA	1 cut(s)	1358
LguI	GCTCTTC	2 cut(s)	227, 239
LmnI	GCTCC	7 cut(s)	183, 200, 277, 1028, 1145, 1453, 1552
Lsp1109I	GCAGC	7 cut(s)	653, 1102, 1259, 1502, 1600, 1829, 1871
LweI	GCATC	1 cut(s)	1477
MaeI	CTAG	5 cut(s)	92, 473, 638, 848, 930
MaeII	ACGT	4 cut(s)	15, 1063, 1183, 1303
MaeIII	GTNAC	3 cut(s)	483, 1189, 1669
MflI	RGATCY	6 cut(s)	310, 662, 786, 961, 1208, 1585
MhlI	GDGCHC	5 cut(s)	197, 213, 451, 679, 1150
MluCI	AATT	9 cut(s)	74, 359, 1277, 1281, 1354, 1396, 1430, 1509, 1524
Mly113I	GGCGCC	1 cut(s)	344
MlyI	GAGTC	5 cut(s)	101, 149, 506, 1427, 1769
MmeI	TCCRAC	2 cut(s)	578, 2016
MroI	TCCGGA	1 cut(s)	1358
MroXI	GAANNNNTTC	1 cut(s)	843
MseI	TTAA	4 cut(s)	503, 692, 1280, 1560
MslI	CAYNNNNRTG	2 cut(s)	1241, 1877
MspA1I	CMGCKG	1 cut(s)	1820
MspI	CCGG	4 cut(s)	281, 1359, 1422, 2004
MspR9I	CCNGG	3 cut(s)	726, 1143, 1423
MvaI	CCWGG	2 cut(s)	726, 1143
MvnI	CGCG	3 cut(s)	35, 1252, 2037
MwoI	GCNNNNNNNGC	4 cut(s)	154, 192, 1613, 1784
NarI	GGCGCC	1 cut(s)	344
NciI	CCSGG	1 cut(s)	1423
NheI	GCTAGC	1 cut(s)	637
NlaIII	CATG	4 cut(s)	406, 648, 1375, 1670
NlaIV	GGNNCC	6 cut(s)	40, 279, 312, 345, 788, 1147
NmeAIII	GCCGAG	1 cut(s)	1573
NmuCI	GTSAC	2 cut(s)	1189, 1669
NspI	RCATGY	1 cut(s)	648
NspV	TTCGAA	1 cut(s)	1975
OliI	CACNNNNGTG	1 cut(s)	1241
PceI	AGGCCT	1 cut(s)	550
PciSI	GCTCTTC	2 cut(s)	227, 239
PcsI	WCGNNNNNNNCGW	2 cut(s)	102, 266
PdmI	GAANNNNTTC	1 cut(s)	843
PfeI	GAWTC	3 cut(s)	380, 1737, 1913
PleI	GAGTC	5 cut(s)	101, 148, 505, 1426, 1768
PluTI	GGCGCC	1 cut(s)	347
PmaCI	CACGTG	1 cut(s)	1304
PmlI	CACGTG	1 cut(s)	1304
PpsI	GAGTC	5 cut(s)	101, 148, 505, 1426, 1768
Ppu21I	YACGTR	1 cut(s)	1304
PshBI	ATTAAT	1 cut(s)	1280
Psp124BI	GAGCTC	1 cut(s)	197
Psp6I	CCWGG	2 cut(s)	724, 1141
PspCI	CACGTG	1 cut(s)	1304
PspGI	CCWGG	2 cut(s)	724, 1141
PspN4I	GGNNCC	6 cut(s)	40, 279, 312, 345, 788, 1147
PspPI	GGNCC	3 cut(s)	354, 512, 1177
PsrI	GAACNNNNNNTAC	2 cut(s)	858, 890
PstI	CTGCAG	1 cut(s)	1582
PsuI	RGATCY	6 cut(s)	310, 662, 786, 961, 1208, 1585
PvuII	CAGCTG	1 cut(s)	1820
RsaI	GTAC	2 cut(s)	772, 1677
RsaNI	GTAC	2 cut(s)	771, 1676
RseI	CAYNNNNRTG	2 cut(s)	1241, 1877
SacI	GAGCTC	1 cut(s)	197
SapI	GCTCTTC	2 cut(s)	227, 239
SaqAI	TTAA	4 cut(s)	503, 692, 1280, 1560
Sau96I	GGNCC	3 cut(s)	354, 512, 1177
ScaI	AGTACT	1 cut(s)	1677
SchI	GAGTC	5 cut(s)	101, 149, 506, 1427, 1769
ScrFI	CCNGG	3 cut(s)	726, 1143, 1423
SduI	GDGCHC	5 cut(s)	197, 213, 451, 679, 1150
SfaNI	GCATC	1 cut(s)	1477
SfcI	CTRYAG	2 cut(s)	957, 1578
SfoI	GGCGCC	1 cut(s)	345
SfuI	TTCGAA	1 cut(s)	1975
SmiMI	CAYNNNNRTG	2 cut(s)	1241, 1877
SmlI	CTYRAG	4 cut(s)	678, 766, 1682, 1729
SmoI	CTYRAG	4 cut(s)	678, 766, 1682, 1729
Sse9I	AATT	9 cut(s)	74, 359, 1277, 1281, 1354, 1396, 1430, 1509, 1524
SseBI	AGGCCT	1 cut(s)	550
SsiI	CCGC	7 cut(s)	35, 102, 157, 227, 320, 1935, 2037
SspDI	GGCGCC	1 cut(s)	343
SspMI	CTAG	5 cut(s)	92, 473, 638, 848, 930
SstI	GAGCTC	1 cut(s)	197
StuI	AGGCCT	1 cut(s)	550
StyD4I	CCNGG	3 cut(s)	724, 1141, 1421
TaaI	ACNGT	5 cut(s)	559, 958, 1675, 1710, 1879
TaiI	ACGT	4 cut(s)	18, 1066, 1186, 1306
TaqII	GACCGA	1 cut(s)	431
TasI	AATT	9 cut(s)	74, 359, 1277, 1281, 1354, 1396, 1430, 1509, 1524
TatI	WGTACW	1 cut(s)	1675
TauI	GCSGC	3 cut(s)	104, 229, 2040
TfiI	GAWTC	3 cut(s)	380, 1737, 1913
Tru1I	TTAA	4 cut(s)	503, 692, 1280, 1560
Tru9I	TTAA	4 cut(s)	503, 692, 1280, 1560
TscAI	CASTG	3 cut(s)	1295, 1770, 1896
TseFI	GTSAC	2 cut(s)	1189, 1669
TseI	GCWGC	7 cut(s)	641, 1115, 1247, 1490, 1613, 1817, 1859
Tsp45I	GTSAC	2 cut(s)	1189, 1669
TspDTI	ATGAA	3 cut(s)	819, 990, 998
TspRI	CASTG	3 cut(s)	1295, 1770, 1896
VspI	ATTAAT	1 cut(s)	1280
XapI	RAATTY	3 cut(s)	1396, 1430, 1509
XceI	RCATGY	1 cut(s)	648
XmnI	GAANNNNTTC	1 cut(s)	843
XspI	CTAG	5 cut(s)	92, 473, 638, 848, 930
ZrmI	AGTACT	1 cut(s)	1677

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.