Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0011483

Physical Location & Seq

Genomic Coordinates Reverse (-)

4935394 .. 4944413

Gene Structure 9020 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00282810.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2466 bp

ATGATCAAAGCGCTCGCTTATCTTCTGCTCCTAGTCGTCTTCGTCTCCACGCCTTCACAAGCCAAATTAAGCATCCTGCAACCCCAACCTGCCGCAGGCTTCAACGTCAGCCATGTACAGATTTATGCACCGCGACTAGATGATCCATCATCAAGAACGTTTGAGAGTTGTTCTACGGACACATTTGAGATATATGGACCCTGTGCATACCAAATCTGCTATGTCTATCTTTACAGAACAGGACCAGATGGTTGGAAACCGGAGAGTGTGAAAATCTATAGTCATAATTCCAGGGCTGTTTCGTTCTACTACAATACCTTCATTCCCAGTGACATTTGGAGAAACCCCTCTCTCTCGCGCCTTCCCTCCCGCACAGATTTAACCAAACCAAGTGCTTATAGCTATTATGCCATCTATAGACGTTTCCGATTATGTGCTGGAGGCTCCGAAGATGGAGAAGAAGATGAAAAAGTCGAAAAAGAGTCCATAACCGACTCTCCGATTAGTGAGAAGAAGAGCAGTAGCAAGAAAGAGTCAAAGCTGAAGAAGCGGAAAGCGCTGGATATGGAGGCCGACGACGAAGACAGGAGCGAGACCAGCTCCGAGCTCGGCGAGCCTGTCAATTTGAAGCCGAACGGCGATTCTTCGGTGGAGAAGAGCAAGAAGAAATCGAAGAAGGCCAAGGTGGAGGAGGAGGAGGAGAAGGTTGAGGTGAAGAAGGATGATCCTAATGCCGTAACCAAGTTTCGGATTTCGGCGCCGTTGAGAGCTAAGCTGAAGGAGAAGGGGATCGAGGCCTTGTTTACGATTCAGGCCATGACGTTCGACACCATTTTGGACGGGGCGGACTTGGTGGGCCGGGCTCGCACCGGTCAGGGTAAAACTCTGGCTTTTGTGTTGCCTATATTGGAGTCGTTAACAAATGGTTCTGCTAAAGAATTTCGAAAGACTGGATACGGCAGAGCACCGACTGTTATTGTGTTTGCGGACTTTGAATTTTATGGTGGTGCTGTGGGGTTAGCGTCGTGTTGTGTATATGGTGGATCTCCTTACGCAGCTCAAGAATATAAGTTGAAGAGAGGGGTTGATGTCATTGTGGGAACACCTGGTCGTATCAAGGATCATATTGAAAGGGGGAATATAGACTTGAGCACTTTGAAGTTTCGGGTCCTTGATGAAGCTGATGAAATGCTGAGGATGGGTTTTGTTGATGACGTTGAACAAATTCTAGGCAAGGTCAAAGATGTAACTAAAGTTCAAACGCTTCTCTTCAGTGCAACCTTACCCTCTTGGGTGCAAAATATTTCCAAAAGGTTTCTTAAATCAGATAAGAAAACTGCAGATCTGGTTGGTAATGAAAAGATGAAGGCCAGCGTCAATGTTAGGCATATTGTTCTTCCTTGCTCTAGTTCAGCTAGAGTAGATGTTATTCCTGATATCATTCGTTGTTATAGCAGTGGAGGTCGCACTATTATTTTCACTGAGACAAAGGATTGTGCTTCTGAGCTTTCTGAGAGGTTGCATGGAGCAAGACCTTTGCACGGGGACATACAGCAAGGTCAACGGGAGGTCACACTTGCAGGTTTCAGGTCTGGCAAATTCTCGACATTAGTAGCCACAAATGTGGCAGCTCGTGGACTGGATATCAATGATGTTCAATTAATTATTCAGTGTGAACCTCCACGTGATGTAGAAGACTATATCCATCGATCCGGACGCACAGGGAGAGCAGGCAATTCTGGAGTTGCTGTAATGCTTTATGACCCTAGAAAGTCAAACATATCAAAGATCGAAAGAGAATCTGGGGTGAAATTTGAGCATGTTTCTGCTCCTCAGCCAATTGACGTTGCCAAAGCTGCAGGTCTTAGTGCAGCAGAAATGATTACTAATGTCTCTGATAGCGTAATTCCTGCATTCAAGAGTGTTGCTGAGGAGCTTCTAAACACCTCAGGCCTATCTGCAGTAGATCTACTGTCAAAGGCTCTTGCTAAGGCTGCTGGCTACACCGATGTAAAGAAGAGGTCACTTCTGAGTTCTATGGAGAACCATGTCACCGTACTGCTTGAAGCTGGAAAGCCCATTTACACAGCATCTTTTGCTTACTCGGCCTTGAGGAGGTTCTTGCCGGAGGAGAAGGTTGAGTCAGTGAAGGGTATGGCTCTAACAACTGATGGAAAGGGTGCAGTGTTTGATGTAGCTGCTGAAGATTTGGATATGTTTCTTACTGGTACTAAACACTTGGTTCACAAAATGCAGCTGGTATCAGCATACAGGTCTTGGACTCTTTGCCGAGTTTACAAGAAAAAGAAGCAAGGGGAGGAAGATTTGGCGGTGGTGGCCGTGGTGGTAGTAGGTTTGGTGGAAGAGGAGGTGGCAGGGGTTTCTCTAATGGAAGAAATGATAGGTTTGGTGGCGGCGGTGGCAGAGGCCGCGGCAACTACAACAGATGGTGAAACCCAGTGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017151 GO:0019899 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko01110 map01110

Pfam Domains

Protein Families

DEAD GUCT Helicase_C

Protein Analysis

821

Amino Acids

89.99

Weight (kDa)

7.49

Isoelectric Point (pI)

37.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ATS3	PF06232	38 - 114	1.6e-29	Embryo-specific protein 3, (ATS3)
DEAD	PF00270	269 - 434	1.8e-33	DEAD/DEAH box helicase
Helicase_C	PF00271	474 - 578	2.5e-27	Helicase conserved C-terminal domain
DD_DDX21-DDX50	PF26142	612 - 664	1.7e-18	DDX21/DDX50 dimerisation domain
GUCT	PF08152	667 - 745	3.4e-23	GUCT (NUC152) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0011483)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G62190
fragaria_vesca	FvH4_7g28410
malus_domestica	MD01G1187800.v1.1 MD07G1259900.v1.1 MD07G1260300.v1.1
prunus_persica	Prupe.2G284200_v2.0.a1 Prupe.2G284200_v2.0.a1 Prupe.2G284200_v2.0.a1
pyrus_communis	pycom07g23330
rosa_chinensis	RchiOBHm_Chr1g0375371
rosa_laevigata	RLG00000026667
rosa_multiflora	Rmu_sc0000554.1_g000041 Rmu_ssc0000106.1_g000003
rosa_roxburghii	Rroxscaffold_4G00282810
rosa_rugosa	Rorug01G0390800
rosa_samantha	Rh1AG401100 Rh1BG363200 Rh1CG376500 Rh1DG394000
rosa_wichuraiana	Rw1G035610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	3 cut(s)	97, 1571, 1850
AccB1I	GGYRCC	1 cut(s)	757
AccII	CGCG	3 cut(s)	133, 358, 2432
AccIII	TCCGGA	1 cut(s)	1712
AclI	AACGTT	1 cut(s)	158
AclWI	GGATC	6 cut(s)	137, 719, 797, 1051, 1128, 1704
AcoI	YGGCCR	1 cut(s)	2337
AcsI	RAATTY	5 cut(s)	938, 995, 1224, 1598, 1811
AcuI	CTGAAG	4 cut(s)	563, 797, 1255, 2223
AcvI	CACGTG	1 cut(s)	1685
AcyI	GRCGYC	1 cut(s)	758
AfaI	GTAC	3 cut(s)	117, 2058, 2230
AfeI	AGCGCT	2 cut(s)	12, 558
AfiI	CCNNNNNNNGG	4 cut(s)	95, 747, 1541, 2115
AgeI	ACCGGT	1 cut(s)	869
AjnI	CCWGG	2 cut(s)	290, 1105
AleI	CACNNNNGTG	1 cut(s)	1622
Alw21I	GWGCWC	3 cut(s)	609, 967, 1154
Alw26I	GTCTC	4 cut(s)	49, 587, 1478, 1897
AlwI	GGATC	6 cut(s)	137, 719, 797, 1051, 1128, 1704
Aor13HI	TCCGGA	1 cut(s)	1712
Aor51HI	AGCGCT	2 cut(s)	12, 558
ApeKI	GCWGC	7 cut(s)	1055, 1628, 1856, 1871, 1994, 2198, 2254
ApoI	RAATTY	5 cut(s)	938, 995, 1224, 1598, 1811
AseI	ATTAAT	1 cut(s)	1661
AsiGI	ACCGGT	1 cut(s)	869
Asp700I	GAANNNNTTC	1 cut(s)	1224
AspLEI	GCGC	4 cut(s)	13, 360, 559, 760
AspS9I	GGNCC	4 cut(s)	197, 242, 856, 1168
AsuC2I	CCSGG	1 cut(s)	860
AsuHPI	GGTGA	4 cut(s)	724, 1819, 2044, 2462
AsuII	TTCGAA	1 cut(s)	943
AvaII	GGWCC	3 cut(s)	197, 242, 1168
AxyI	CCTNAGG	1 cut(s)	1948
BanI	GGYRCC	1 cut(s)	757
BanII	GRGCYC	2 cut(s)	609, 865
BauI	CACGAG	1 cut(s)	1632
BbrPI	CACGTG	1 cut(s)	1685
BbsI	GAAGAC	3 cut(s)	31, 588, 1701
Bbv12I	GWGCWC	3 cut(s)	609, 967, 1154
BbvCI	CCTCAGC	3 cut(s)	1193, 1833, 1929
BbvI	GCAGC	7 cut(s)	1067, 1640, 1843, 1883, 1981, 2185, 2266
BccI	CCATC	8 cut(s)	154, 242, 419, 446, 1192, 1713, 2165, 2441
BceAI	ACGGC	5 cut(s)	652, 719, 745, 973, 2324
BciT130I	CCWGG	2 cut(s)	292, 1107
BciVI	GTATCC	1 cut(s)	947
BclI	TGATCA	1 cut(s)	3
BcnI	CCSGG	1 cut(s)	860
BcoDI	GTCTC	4 cut(s)	49, 587, 1478, 1897
BfaI	CTAG	6 cut(s)	32, 137, 1229, 1407, 1416, 1767
BfmI	CTRYAG	5 cut(s)	277, 415, 1338, 1857, 1959
BfoI	RGCGCY	3 cut(s)	14, 560, 761
BfuAI	ACCTGC	3 cut(s)	97, 1571, 1850
BfuI	GTATCC	1 cut(s)	947
BglII	AGATCT	2 cut(s)	1342, 1966
BlpI	GCTNAGC	1 cut(s)	771
Bme1390I	CCNGG	3 cut(s)	292, 860, 1107
Bme18I	GGWCC	3 cut(s)	197, 242, 1168
BmgT120I	GGNCC	4 cut(s)	197, 242, 856, 1168
BmiI	GGNNCC	4 cut(s)	199, 445, 759, 1169
BmrFI	CCNGG	3 cut(s)	292, 860, 1107
BmrI	ACTGGG	2 cut(s)	321, 2452
BmsI	GCATC	2 cut(s)	81, 2099
BmuI	ACTGGG	2 cut(s)	321, 2452
BpiI	GAAGAC	3 cut(s)	31, 588, 1701
BplI	GAGNNNNNCTC	2 cut(s)	584, 616
BpmI	CTGGAG	2 cut(s)	459, 1761
Bpu10I	CCTNAGC	4 cut(s)	1193, 1833, 1929, 1989
Bpu1102I	GCTNAGC	1 cut(s)	771
Bpu14I	TTCGAA	1 cut(s)	943
BpuEI	CTTGAG	3 cut(s)	1044, 1168, 2131
BpuMI	CCSGG	1 cut(s)	860
Bsa29I	ATCGAT	1 cut(s)	1708
BsaAI	YACGTR	1 cut(s)	1685
BsaHI	GRCGYC	1 cut(s)	758
BsaI	GGTCTC	1 cut(s)	587
BsaJI	CCNNGG	4 cut(s)	291, 681, 2340, 2430
BsaWI	WCCGGW	3 cut(s)	259, 869, 1712
BsaXI	ACNNNNNCTCC	4 cut(s)	481, 511, 1734, 1764
Bsc4I	CCNNNNNNNGG	4 cut(s)	95, 747, 1541, 2115
Bse118I	RCCGGY	1 cut(s)	869
Bse1I	ACTGG	5 cut(s)	327, 955, 1644, 2230, 2458
Bse21I	CCTNAGG	1 cut(s)	1948
BseAI	TCCGGA	1 cut(s)	1712
BseBI	CCWGG	2 cut(s)	292, 1107
BseCI	ATCGAT	1 cut(s)	1708
BseDI	CCNNGG	4 cut(s)	291, 681, 2340, 2430
BseGI	GGATG	3 cut(s)	72, 727, 1203
BseLI	CCNNNNNNNGG	4 cut(s)	95, 747, 1541, 2115
BseMII	CTCAG	8 cut(s)	1184, 1473, 1494, 1503, 1847, 1920, 1962, 2021
BseNI	ACTGG	5 cut(s)	327, 955, 1644, 2230, 2458
BseRI	GAGGAG	9 cut(s)	704, 707, 710, 713, 1821, 1946, 2128, 2144, 2381
BseXI	GCAGC	7 cut(s)	1067, 1640, 1843, 1883, 1981, 2185, 2266
BsgI	GTGCAG	2 cut(s)	1890, 2202
Bsh1236I	CGCG	3 cut(s)	133, 358, 2432
BshNI	GGYRCC	1 cut(s)	757
BshTI	ACCGGT	1 cut(s)	869
BshVI	ATCGAT	1 cut(s)	1708
BsiHKAI	GWGCWC	3 cut(s)	609, 967, 1154
BsiSI	CCGG	5 cut(s)	260, 859, 870, 1713, 2126
BslFI	GGGAC	1 cut(s)	1559
BslI	CCNNNNNNNGG	4 cut(s)	95, 747, 1541, 2115
BsmAI	GTCTC	4 cut(s)	49, 587, 1478, 1897
BsmBI	CGTCTC	1 cut(s)	49
BsmFI	GGGAC	1 cut(s)	1559
BsmI	GAATGC	1 cut(s)	1913
Bso31I	GGTCTC	1 cut(s)	587
Bsp119I	TTCGAA	1 cut(s)	943
Bsp1286I	GDGCHC	4 cut(s)	609, 865, 967, 1154
Bsp13I	TCCGGA	1 cut(s)	1712
Bsp1407I	TGTACA	1 cut(s)	115
Bsp1720I	GCTNAGC	1 cut(s)	771
BspCNI	CTCAG	8 cut(s)	1185, 1474, 1495, 1504, 1846, 1921, 1961, 2022
BspDI	ATCGAT	1 cut(s)	1708
BspEI	TCCGGA	1 cut(s)	1712
BspFNI	CGCG	3 cut(s)	133, 358, 2432
BspLI	GGNNCC	4 cut(s)	199, 445, 759, 1169
BspMAI	CTGCAG	3 cut(s)	1342, 1861, 1963
BspMI	ACCTGC	3 cut(s)	97, 1571, 1850
BspPI	GGATC	6 cut(s)	137, 719, 797, 1051, 1128, 1704
BspQI	GCTCTTC	2 cut(s)	509, 650
BspT104I	TTCGAA	1 cut(s)	943
BspT107I	GGYRCC	1 cut(s)	757
BspTNI	GGTCTC	1 cut(s)	587
BsrFI	RCCGGY	1 cut(s)	869
BsrGI	TGTACA	1 cut(s)	115
BsrI	ACTGG	5 cut(s)	327, 955, 1644, 2230, 2458
BssAI	RCCGGY	1 cut(s)	869
BssECI	CCNNGG	4 cut(s)	291, 681, 2340, 2430
BssNI	GRCGYC	1 cut(s)	758
BssSI	CACGAG	1 cut(s)	1632
BssT1I	CCWWGG	1 cut(s)	681
Bst2BI	CACGAG	1 cut(s)	1632
Bst2UI	CCWGG	2 cut(s)	292, 1107
Bst4CI	ACNGT	3 cut(s)	973, 1974, 2056
Bst6I	CTCTTC	6 cut(s)	509, 650, 1070, 1274, 2012, 2358
BstACI	GRCGYC	1 cut(s)	758
BstAPI	GCANNNNNTGC	1 cut(s)	2096
BstAUI	TGTACA	1 cut(s)	115
BstBAI	YACGTR	1 cut(s)	1685
BstBI	TTCGAA	1 cut(s)	943
BstC8I	GCNNGC	7 cut(s)	15, 97, 614, 865, 1372, 1732, 1999
BstDSI	CCRYGG	2 cut(s)	2340, 2430
BstENI	CCTNNNNNAGG	2 cut(s)	93, 2113
BstF5I	GGATG	3 cut(s)	72, 727, 1203
BstFNI	CGCG	3 cut(s)	133, 358, 2432
BstH2I	RGCGCY	3 cut(s)	14, 560, 761
BstHHI	GCGC	4 cut(s)	13, 360, 559, 760
BstMAI	GTCTC	4 cut(s)	49, 587, 1478, 1897
BstNI	CCWGG	2 cut(s)	292, 1107
BstNSI	RCATGY	1 cut(s)	1823
BstSCI	CCNGG	3 cut(s)	290, 858, 1105
BstSFI	CTRYAG	5 cut(s)	277, 415, 1338, 1857, 1959
BstUI	CGCG	3 cut(s)	133, 358, 2432
BstV1I	GCAGC	7 cut(s)	1067, 1640, 1843, 1883, 1981, 2185, 2266
BstV2I	GAAGAC	3 cut(s)	31, 588, 1701
BstX2I	RGATCY	3 cut(s)	1043, 1342, 1966
BstXI	CCANNNNNNTGG	2 cut(s)	252, 1624
BstYI	RGATCY	3 cut(s)	1043, 1342, 1966
Bsu15I	ATCGAT	1 cut(s)	1708
Bsu36I	CCTNAGG	1 cut(s)	1948
BsuI	GTATCC	1 cut(s)	947
BsuTUI	ATCGAT	1 cut(s)	1708
BtgI	CCRYGG	2 cut(s)	2340, 2430
BtsCI	GGATG	3 cut(s)	72, 727, 1203
BtsI	GCAGTG	2 cut(s)	1462, 2190
BtsIMutI	CAGTG	8 cut(s)	334, 1279, 1462, 1479, 1676, 2151, 2190, 2465
BveI	ACCTGC	3 cut(s)	97, 1571, 1850
Cac8I	GCNNGC	7 cut(s)	15, 97, 614, 865, 1372, 1732, 1999
CfoI	GCGC	4 cut(s)	13, 360, 559, 760
Cfr10I	RCCGGY	1 cut(s)	869
Cfr13I	GGNCC	4 cut(s)	197, 242, 856, 1168
Cfr42I	CCGCGG	1 cut(s)	2433
ClaI	ATCGAT	1 cut(s)	1708
CseI	GACGC	3 cut(s)	1011, 1363, 1725
CsiI	ACCWGGT	1 cut(s)	1105
Csp6I	GTAC	3 cut(s)	116, 2057, 2229
CspAI	ACCGGT	1 cut(s)	869
CviAII	CATG	5 cut(s)	113, 817, 1523, 1820, 2048
CviQI	GTAC	3 cut(s)	116, 2057, 2229
DinI	GGCGCC	1 cut(s)	759
EaeI	YGGCCR	1 cut(s)	2337
Eam1104I	CTCTTC	6 cut(s)	509, 650, 1070, 1274, 2012, 2358
EarI	CTCTTC	6 cut(s)	509, 650, 1070, 1274, 2012, 2358
EciI	GGCGGA	1 cut(s)	860
Ecl136II	GAGCTC	1 cut(s)	607
Eco130I	CCWWGG	1 cut(s)	681
Eco147I	AGGCCT	2 cut(s)	797, 1953
Eco24I	GRGCYC	2 cut(s)	609, 865
Eco31I	GGTCTC	1 cut(s)	587
Eco32I	GATATC	2 cut(s)	1438, 1645
Eco47I	GGWCC	3 cut(s)	197, 242, 1168
Eco47III	AGCGCT	2 cut(s)	12, 558
Eco53kI	GAGCTC	1 cut(s)	607
Eco57I	CTGAAG	4 cut(s)	563, 797, 1255, 2223
Eco72I	CACGTG	1 cut(s)	1685
Eco81I	CCTNAGG	1 cut(s)	1948
EcoICRI	GAGCTC	1 cut(s)	607
EcoNI	CCTNNNNNAGG	2 cut(s)	93, 2113
EcoO109I	RGGNCCY	1 cut(s)	1168
EcoRII	CCWGG	2 cut(s)	290, 1105
EcoRV	GATATC	2 cut(s)	1438, 1645
EcoT14I	CCWWGG	1 cut(s)	681
EcoT38I	GRGCYC	2 cut(s)	609, 865
EgeI	GGCGCC	1 cut(s)	759
EheI	GGCGCC	1 cut(s)	759
ErhI	CCWWGG	1 cut(s)	681
Esp3I	CGTCTC	1 cut(s)	49
FaeI	CATG	5 cut(s)	116, 820, 1526, 1823, 2051
FaqI	GGGAC	1 cut(s)	1559
FatI	CATG	5 cut(s)	112, 816, 1522, 1819, 2047
FauI	CCCGC	1 cut(s)	377
FbaI	TGATCA	1 cut(s)	3
FokI	GGATG	3 cut(s)	59, 734, 1210
FriOI	GRGCYC	2 cut(s)	609, 865
FspBI	CTAG	6 cut(s)	32, 137, 1229, 1407, 1416, 1767
GlaI	GCGC	4 cut(s)	12, 359, 558, 759
GsuI	CTGGAG	2 cut(s)	459, 1761
HaeII	RGCGCY	3 cut(s)	14, 560, 761
HapII	CCGG	5 cut(s)	260, 859, 870, 1713, 2126
HgaI	GACGC	3 cut(s)	1011, 1363, 1725
HhaI	GCGC	4 cut(s)	13, 360, 559, 760
Hin1I	GRCGYC	1 cut(s)	758
Hin1II	CATG	5 cut(s)	116, 820, 1526, 1823, 2051
Hin6I	GCGC	4 cut(s)	11, 358, 557, 758
HinP1I	GCGC	4 cut(s)	11, 358, 557, 758
HincII	GTYRAC	2 cut(s)	918, 1562
HindII	GTYRAC	2 cut(s)	918, 1562
HinfI	GANTC	9 cut(s)	482, 494, 533, 641, 808, 911, 1799, 2141, 2281
HpaI	GTTAAC	1 cut(s)	918
HpaII	CCGG	5 cut(s)	260, 859, 870, 1713, 2126
HphI	GGTGA	4 cut(s)	724, 1819, 2044, 2462
Hpy166II	GTNNAC	7 cut(s)	804, 918, 1562, 1637, 1676, 2245, 2296
Hpy188III	TCNNGA	7 cut(s)	153, 1061, 1433, 1603, 1713, 1740, 1918
Hpy8I	GTNNAC	7 cut(s)	804, 918, 1562, 1637, 1676, 2245, 2296
Hpy99I	CGWCG	3 cut(s)	578, 581, 1027
HpyCH4III	ACNGT	3 cut(s)	973, 1974, 2056
HpyCH4IV	ACGT	7 cut(s)	105, 158, 421, 821, 1215, 1684, 1845
HpySE526I	ACGT	7 cut(s)	105, 158, 421, 821, 1215, 1684, 1845
Hsp92I	GRCGYC	1 cut(s)	758
Hsp92II	CATG	5 cut(s)	116, 820, 1526, 1823, 2051
HspAI	GCGC	4 cut(s)	11, 358, 557, 758
KasI	GGCGCC	1 cut(s)	757
Kpn2I	TCCGGA	1 cut(s)	1712
Ksp22I	TGATCA	1 cut(s)	3
KspAI	GTTAAC	1 cut(s)	918
KspI	CCGCGG	1 cut(s)	2433
LguI	GCTCTTC	2 cut(s)	509, 650
LmnI	GCTCC	7 cut(s)	33, 449, 588, 605, 1526, 1834, 1933
Lsp1109I	GCAGC	7 cut(s)	1067, 1640, 1843, 1883, 1981, 2185, 2266
LweI	GCATC	2 cut(s)	81, 2099
MabI	ACCWGGT	1 cut(s)	1105
MaeI	CTAG	6 cut(s)	32, 137, 1229, 1407, 1416, 1767
MaeII	ACGT	7 cut(s)	105, 158, 421, 821, 1215, 1684, 1845
MaeIII	GTNAC	6 cut(s)	329, 736, 1246, 1570, 2022, 2050
MfeI	CAATTG	1 cut(s)	1839
MflI	RGATCY	3 cut(s)	1043, 1342, 1966
MhlI	GDGCHC	4 cut(s)	609, 865, 967, 1154
Mly113I	GGCGCC	1 cut(s)	758
MlyI	GAGTC	6 cut(s)	488, 491, 542, 920, 2150, 2275
MmeI	TCCRAC	1 cut(s)	233
MroI	TCCGGA	1 cut(s)	1712
MroXI	GAANNNNTTC	1 cut(s)	1224
MseI	TTAA	6 cut(s)	68, 380, 917, 1320, 1661, 2464
MslI	CAYNNNNRTG	1 cut(s)	1622
MspA1I	CMGCKG	2 cut(s)	2257, 2432
MspI	CCGG	5 cut(s)	260, 859, 870, 1713, 2126
MspR9I	CCNGG	3 cut(s)	292, 860, 1107
MunI	CAATTG	1 cut(s)	1839
Mva1269I	GAATGC	1 cut(s)	1913
MvaI	CCWGG	2 cut(s)	292, 1107
MvnI	CGCG	3 cut(s)	133, 358, 2432
NarI	GGCGCC	1 cut(s)	758
NciI	CCSGG	1 cut(s)	860
NlaIII	CATG	5 cut(s)	116, 820, 1526, 1823, 2051
NlaIV	GGNNCC	4 cut(s)	199, 445, 759, 1169
NmeAIII	GCCGAG	3 cut(s)	588, 2084, 2315
NmuCI	GTSAC	4 cut(s)	329, 1570, 2022, 2050
NspI	RCATGY	1 cut(s)	1823
NspV	TTCGAA	1 cut(s)	943
OliI	CACNNNNGTG	1 cut(s)	1622
PceI	AGGCCT	2 cut(s)	797, 1953
PciSI	GCTCTTC	2 cut(s)	509, 650
PctI	GAATGC	1 cut(s)	1913
PdmI	GAANNNNTTC	1 cut(s)	1224
PfeI	GAWTC	3 cut(s)	641, 808, 1799
PinAI	ACCGGT	1 cut(s)	869
PleI	GAGTC	6 cut(s)	488, 490, 541, 919, 2149, 2275
PluTI	GGCGCC	1 cut(s)	761
PmaCI	CACGTG	1 cut(s)	1685
PmlI	CACGTG	1 cut(s)	1685
PpsI	GAGTC	6 cut(s)	488, 490, 541, 919, 2149, 2275
Ppu21I	YACGTR	1 cut(s)	1685
PpuMI	RGGWCCY	1 cut(s)	1168
PshBI	ATTAAT	1 cut(s)	1661
Psp124BI	GAGCTC	1 cut(s)	609
Psp1406I	AACGTT	1 cut(s)	158
Psp5II	RGGWCCY	1 cut(s)	1168
Psp6I	CCWGG	2 cut(s)	290, 1105
PspCI	CACGTG	1 cut(s)	1685
PspGI	CCWGG	2 cut(s)	290, 1105
PspN4I	GGNNCC	4 cut(s)	199, 445, 759, 1169
PspPI	GGNCC	4 cut(s)	197, 242, 856, 1168
PspPPI	RGGWCCY	1 cut(s)	1168
PsrI	GAACNNNNNNTAC	2 cut(s)	1239, 1271
PstI	CTGCAG	3 cut(s)	1342, 1861, 1963
PsuI	RGATCY	3 cut(s)	1043, 1342, 1966
PvuII	CAGCTG	1 cut(s)	2257
RsaI	GTAC	3 cut(s)	117, 2058, 2230
RsaNI	GTAC	3 cut(s)	116, 2057, 2229
RseI	CAYNNNNRTG	1 cut(s)	1622
SacI	GAGCTC	1 cut(s)	609
SacII	CCGCGG	1 cut(s)	2433
SapI	GCTCTTC	2 cut(s)	509, 650
SaqAI	TTAA	6 cut(s)	68, 380, 917, 1320, 1661, 2464
Sau96I	GGNCC	4 cut(s)	197, 242, 856, 1168
SchI	GAGTC	6 cut(s)	488, 491, 542, 920, 2150, 2275
ScrFI	CCNGG	3 cut(s)	292, 860, 1107
SduI	GDGCHC	4 cut(s)	609, 865, 967, 1154
SexAI	ACCWGGT	1 cut(s)	1105
SfaNI	GCATC	2 cut(s)	81, 2099
SfcI	CTRYAG	5 cut(s)	277, 415, 1338, 1857, 1959
SfoI	GGCGCC	1 cut(s)	759
Sfr303I	CCGCGG	1 cut(s)	2433
SfuI	TTCGAA	1 cut(s)	943
SgrBI	CCGCGG	1 cut(s)	2433
SinI	GGWCC	3 cut(s)	197, 242, 1168
SmiMI	CAYNNNNRTG	1 cut(s)	1622
SmlI	CTYRAG	3 cut(s)	1059, 1147, 2110
SmoI	CTYRAG	3 cut(s)	1059, 1147, 2110
SseBI	AGGCCT	2 cut(s)	797, 1953
SspDI	GGCGCC	1 cut(s)	757
SspI	AATATT	1 cut(s)	1303
SspMI	CTAG	6 cut(s)	32, 137, 1229, 1407, 1416, 1767
SstI	GAGCTC	1 cut(s)	609
StuI	AGGCCT	2 cut(s)	797, 1953
StyD4I	CCNGG	3 cut(s)	290, 858, 1105
StyI	CCWWGG	1 cut(s)	681
TaaI	ACNGT	3 cut(s)	973, 1974, 2056
TaiI	ACGT	7 cut(s)	108, 161, 424, 824, 1218, 1687, 1848
TaqI	TCGA	8 cut(s)	474, 671, 792, 825, 943, 1604, 1708, 1791
TatI	WGTACW	1 cut(s)	115
TauI	GCSGC	4 cut(s)	95, 2417, 2432, 2435
TfiI	GAWTC	3 cut(s)	641, 808, 1799
Tru1I	TTAA	6 cut(s)	68, 380, 917, 1320, 1661, 2464
Tru9I	TTAA	6 cut(s)	68, 380, 917, 1320, 1661, 2464
TscAI	CASTG	8 cut(s)	334, 1279, 1462, 1486, 1676, 2151, 2190, 2465
TseFI	GTSAC	4 cut(s)	329, 1570, 2022, 2050
TseI	GCWGC	7 cut(s)	1055, 1628, 1856, 1871, 1994, 2198, 2254
Tsp45I	GTSAC	4 cut(s)	329, 1570, 2022, 2050
TspDTI	ATGAA	6 cut(s)	310, 480, 1191, 1200, 1371, 1379
TspGWI	ACGGA	1 cut(s)	191
TspRI	CASTG	8 cut(s)	334, 1279, 1462, 1486, 1676, 2151, 2190, 2465
VpaK11BI	GGWCC	3 cut(s)	197, 242, 1168
VspI	ATTAAT	1 cut(s)	1661
XagI	CCTNNNNNAGG	2 cut(s)	93, 2113
XapI	RAATTY	5 cut(s)	938, 995, 1224, 1598, 1811
XceI	RCATGY	1 cut(s)	1823
XmnI	GAANNNNTTC	1 cut(s)	1224
XspI	CTAG	6 cut(s)	32, 137, 1229, 1407, 1416, 1767

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.