MD07G1260300.v1.1

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0011483
Physical Location & Seq
Forward (+)
32630446 .. 32632189
1744 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1260300.v1.1.491

Sequence Viewer

Length: 714 bp
ATGGAGTCGTTCGAAAAGAAGTTTCTATGGCCCCCTTGCTCATTTAACACTGCATCCGGGTGCCGATGGAGATACAGCGACCCTTGCTCACTTATCACTGTGCTCGGGCCACACAACGTGCCACTTACGGATGACGCCTTAGCTTTAGCGCGATTTCGTTCTGTATTCATTTTCATAAGGATTCGACGTAATCATGGAGTGCCGGCTGTCGACTACCTGACGCCCTCCCCCTCCTCCTTTATCCGGGCTTGGGACCGGCAATGTAAGATAAACTTACACAGGCAGAGTTTGGCTTTTAGACTAAGGAGAGGGGTGGATATTGTTATTGGAACGCCTAGCCGTATACAGGATCATATAGAGAGGAGCAATATTGACTCGAGTACCGTGAAAGTTAGGATCCTTGACGAAGCTGATGAAATGCTGTGGATGGGCTTTGTTGATGATATTGAACTCATTCTAGTTCAAACGCTTCTCTTCAGTGCAACCTTGCCAAGTTGGCTGAAAGGTATTTCTTTTAGGTTTCTCAAACCAGATAAGAAAACTGTAGATCTTGTTGGTAATGAAAAAATGAAGGCCAGCACCAATGTTGGGCATATTGTTCTTTCTTGCTCCAGTTCAGCAAGATTGCAACTTATTCCTGATATCATAGATTGTTATAGCAGGCCGCACAATCATTTTCACCGAGACAAAGGAAGCTGCTTCTGGACTTGTTGA

Protein Analysis

238

Amino Acids

27.14

Weight (kDa)

9.1

Isoelectric Point (pI)

51.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 99 - 167 5.5e-13 DEAD/DEAH box helicase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 60
AccI GTMKAC 2 cut(s) 210, 343
AccII CGCG 1 cut(s) 151
AciI CCGC 1 cut(s) 665
AclWI GGATC 3 cut(s) 357, 391, 404
AcuI CTGAAG 1 cut(s) 460
AcyI GRCGYC 2 cut(s) 135, 221
AdeI CACNNNGTG 1 cut(s) 118
AfaI GTAC 1 cut(s) 382
AfiI CCNNNNNNNGG 4 cut(s) 243, 250, 346, 588
AgsI TTSAA 2 cut(s) 449, 464
AluBI AGCT 3 cut(s) 143, 410, 696
AluI AGCT 3 cut(s) 143, 410, 696
Alw21I GWGCWC 1 cut(s) 105
Alw26I GTCTC 1 cut(s) 678
AlwI GGATC 3 cut(s) 357, 391, 404
Ama87I CYCGRG 2 cut(s) 104, 376
AoxI GGCC 4 cut(s) 29, 107, 573, 662
ApeKI GCWGC 1 cut(s) 696
Asp700I GAANNNNTTC 1 cut(s) 453
AspLEI GCGC 1 cut(s) 151
AspS9I GGNCC 3 cut(s) 30, 107, 253
AsuC2I CCSGG 2 cut(s) 58, 245
AsuHPI GGTGA 1 cut(s) 671
AsuII TTCGAA 1 cut(s) 12
AvaI CYCGRG 2 cut(s) 104, 376
AvaII GGWCC 1 cut(s) 253
BaeI ACNNNNGTAYC 2 cut(s) 364, 397
BamHI GGATCC 1 cut(s) 396
BanI GGYRCC 1 cut(s) 60
Bbv12I GWGCWC 1 cut(s) 105
BbvI GCAGC 1 cut(s) 683
BccI CCATC 2 cut(s) 60, 421
BceAI ACGGC 1 cut(s) 324
BcnI CCSGG 2 cut(s) 58, 245
BcoDI GTCTC 1 cut(s) 678
BfaI CTAG 2 cut(s) 336, 458
BfmI CTRYAG 1 cut(s) 543
BglI GCCNNNNNGGC 1 cut(s) 496
BglII AGATCT 1 cut(s) 547
BisI GCNGC 2 cut(s) 665, 697
BlsI GCNGC 2 cut(s) 666, 698
Bme1390I CCNGG 2 cut(s) 58, 245
Bme18I GGWCC 1 cut(s) 253
BmeT110I CYCGRG 2 cut(s) 104, 376
BmgT120I GGNCC 3 cut(s) 30, 107, 253
BmiI GGNNCC 4 cut(s) 32, 62, 254, 398
BmrFI CCNGG 2 cut(s) 58, 245
BmsI GCATC 1 cut(s) 62
BpmI CTGGAG 1 cut(s) 595
Bpu10I CCTNAGC 1 cut(s) 139
Bpu14I TTCGAA 1 cut(s) 12
BpuMI CCSGG 2 cut(s) 58, 245
BsaHI GRCGYC 2 cut(s) 135, 221
Bsc4I CCNNNNNNNGG 4 cut(s) 243, 250, 346, 588
Bse118I RCCGGY 2 cut(s) 202, 255
Bse1I ACTGG 1 cut(s) 612
Bse3DI GCAATG 1 cut(s) 266
BseGI GGATG 3 cut(s) 53, 136, 432
BseLI CCNNNNNNNGG 4 cut(s) 243, 250, 346, 588
BseMI GCAATG 1 cut(s) 266
BseNI ACTGG 1 cut(s) 612
BseRI GAGGAG 2 cut(s) 223, 376
BseXI GCAGC 1 cut(s) 683
Bsh1236I CGCG 1 cut(s) 151
BshFI GGCC 4 cut(s) 31, 109, 575, 664
BshNI GGYRCC 1 cut(s) 60
BsiHKAI GWGCWC 1 cut(s) 105
BsiHKCI CYCGRG 2 cut(s) 104, 376
BsiSI CCGG 4 cut(s) 57, 203, 244, 256
BslFI GGGAC 1 cut(s) 266
BslI CCNNNNNNNGG 4 cut(s) 243, 250, 346, 588
BsmAI GTCTC 1 cut(s) 678
BsmFI GGGAC 1 cut(s) 266
BsnI GGCC 4 cut(s) 31, 109, 575, 664
BsoBI CYCGRG 2 cut(s) 104, 376
Bsp119I TTCGAA 1 cut(s) 12
Bsp1286I GDGCHC 1 cut(s) 105
Bsp143I GATC 3 cut(s) 349, 396, 547
BspACI CCGC 1 cut(s) 665
BspANI GGCC 4 cut(s) 31, 109, 575, 664
BspFNI CGCG 1 cut(s) 151
BspLI GGNNCC 4 cut(s) 32, 62, 254, 398
BspPI GGATC 3 cut(s) 357, 391, 404
BspT104I TTCGAA 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 60
BsrDI GCAATG 1 cut(s) 266
BsrFI RCCGGY 2 cut(s) 202, 255
BsrI ACTGG 1 cut(s) 612
BssAI RCCGGY 2 cut(s) 202, 255
BssMI GATC 3 cut(s) 349, 396, 547
BssNAI GTATAC 1 cut(s) 344
BssNI GRCGYC 2 cut(s) 135, 221
Bst1107I GTATAC 1 cut(s) 344
Bst4CI ACNGT 3 cut(s) 100, 385, 544
Bst6I CTCTTC 1 cut(s) 479
BstACI GRCGYC 2 cut(s) 135, 221
BstBI TTCGAA 1 cut(s) 12
BstC8I GCNNGC 3 cut(s) 204, 577, 662
BstDEI CTNAG 2 cut(s) 139, 302
BstF5I GGATG 3 cut(s) 53, 136, 432
BstFNI CGCG 1 cut(s) 151
BstHHI GCGC 1 cut(s) 151
BstKTI GATC 3 cut(s) 352, 399, 550
BstMAI GTCTC 1 cut(s) 678
BstMBI GATC 3 cut(s) 349, 396, 547
BstMWI GCNNNNNNNGC 2 cut(s) 84, 496
BstSCI CCNGG 2 cut(s) 56, 243
BstSFI CTRYAG 1 cut(s) 543
BstUI CGCG 1 cut(s) 151
BstV1I GCAGC 1 cut(s) 683
BstX2I RGATCY 2 cut(s) 396, 547
BstYI RGATCY 2 cut(s) 396, 547
BstZ17I GTATAC 1 cut(s) 344
BsuRI GGCC 4 cut(s) 31, 109, 575, 664
BtsCI GGATG 3 cut(s) 53, 136, 432
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 3 cut(s) 48, 96, 484
Cac8I GCNNGC 3 cut(s) 204, 577, 662
CfoI GCGC 1 cut(s) 151
Cfr10I RCCGGY 2 cut(s) 202, 255
Cfr13I GGNCC 3 cut(s) 30, 107, 253
CseI GACGC 2 cut(s) 143, 229
Csp6I GTAC 1 cut(s) 381
CviAII CATG 1 cut(s) 194
CviQI GTAC 1 cut(s) 381
DdeI CTNAG 2 cut(s) 139, 302
DpnI GATC 3 cut(s) 351, 398, 549
DpnII GATC 3 cut(s) 349, 396, 547
DraIII CACNNNGTG 1 cut(s) 118
Eam1104I CTCTTC 1 cut(s) 479
EarI CTCTTC 1 cut(s) 479
Eco32I GATATC 1 cut(s) 643
Eco47I GGWCC 1 cut(s) 253
Eco57I CTGAAG 1 cut(s) 460
Eco88I CYCGRG 2 cut(s) 104, 376
EcoRV GATATC 1 cut(s) 643
FaeI CATG 1 cut(s) 197
FaiI YATR 9 cut(s) 28, 176, 195, 344, 354, 356, 594, 647, 657
FalI AAGNNNNNCTT 2 cut(s) 257, 289
FaqI GGGAC 1 cut(s) 266
FatI CATG 1 cut(s) 193
FblI GTMKAC 2 cut(s) 210, 343
Fnu4HI GCNGC 2 cut(s) 665, 697
FokI GGATG 3 cut(s) 40, 143, 439
Fsp4HI GCNGC 2 cut(s) 665, 697
FspBI CTAG 2 cut(s) 336, 458
GlaI GCGC 1 cut(s) 150
GluI GCNGC 2 cut(s) 665, 697
GsuI CTGGAG 1 cut(s) 595
HaeIII GGCC 4 cut(s) 31, 109, 575, 664
HapII CCGG 4 cut(s) 57, 203, 244, 256
HgaI GACGC 2 cut(s) 143, 229
HhaI GCGC 1 cut(s) 151
Hin1I GRCGYC 2 cut(s) 135, 221
Hin1II CATG 1 cut(s) 197
Hin6I GCGC 1 cut(s) 149
HinP1I GCGC 1 cut(s) 149
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HinfI GANTC 3 cut(s) 5, 181, 374
HpaII CCGG 4 cut(s) 57, 203, 244, 256
HphI GGTGA 1 cut(s) 671
Hpy166II GTNNAC 2 cut(s) 211, 344
Hpy188III TCNNGA 2 cut(s) 638, 703
Hpy8I GTNNAC 2 cut(s) 211, 344
Hpy99I CGWCG 1 cut(s) 189
HpyAV CCTTC 1 cut(s) 565
HpyCH4III ACNGT 3 cut(s) 100, 385, 544
HpyCH4IV ACGT 2 cut(s) 117, 187
HpyCH4V TGCA 3 cut(s) 53, 482, 628
HpyF10VI GCNNNNNNNGC 2 cut(s) 84, 496
HpyF3I CTNAG 2 cut(s) 139, 302
HpySE526I ACGT 2 cut(s) 117, 187
Hsp92I GRCGYC 2 cut(s) 135, 221
Hsp92II CATG 1 cut(s) 197
HspAI GCGC 1 cut(s) 149
KroI GCCGGC 1 cut(s) 202
KroNI GCCGGC 1 cut(s) 204
Kzo9I GATC 3 cut(s) 349, 396, 547
LmnI GCTCC 2 cut(s) 363, 614
Lsp1109I GCAGC 1 cut(s) 683
LweI GCATC 1 cut(s) 62
MaeI CTAG 2 cut(s) 336, 458
MaeII ACGT 2 cut(s) 117, 187
MalI GATC 3 cut(s) 351, 398, 549
MboI GATC 3 cut(s) 349, 396, 547
MboII GAAGA 1 cut(s) 466
MflI RGATCY 2 cut(s) 396, 547
MhlI GDGCHC 1 cut(s) 105
MlyI GAGTC 2 cut(s) 14, 368
MnlI CCTC 5 cut(s) 235, 241, 244, 302, 354
MroNI GCCGGC 1 cut(s) 202
MroXI GAANNNNTTC 1 cut(s) 453
MseI TTAA 1 cut(s) 45
MslI CAYNNNNRTG 1 cut(s) 58
MspI CCGG 4 cut(s) 57, 203, 244, 256
MspR9I CCNGG 2 cut(s) 58, 245
MvnI CGCG 1 cut(s) 151
MwoI GCNNNNNNNGC 2 cut(s) 84, 496
NaeI GCCGGC 1 cut(s) 204
NciI CCSGG 2 cut(s) 58, 245
NdeII GATC 3 cut(s) 349, 396, 547
NgoMIV GCCGGC 1 cut(s) 202
NlaIII CATG 1 cut(s) 197
NlaIV GGNNCC 4 cut(s) 32, 62, 254, 398
NspV TTCGAA 1 cut(s) 12
PaeR7I CTCGAG 1 cut(s) 376
PdiI GCCGGC 1 cut(s) 204
PdmI GAANNNNTTC 1 cut(s) 453
PfeI GAWTC 1 cut(s) 181
PkrI GCNGC 2 cut(s) 666, 698
PleI GAGTC 2 cut(s) 13, 368
PpsI GAGTC 2 cut(s) 13, 368
PspN4I GGNNCC 4 cut(s) 32, 62, 254, 398
PspPI GGNCC 3 cut(s) 30, 107, 253
PspXI VCTCGAGB 1 cut(s) 376
PsuI RGATCY 2 cut(s) 396, 547
RsaI GTAC 1 cut(s) 382
RsaNI GTAC 1 cut(s) 381
RseI CAYNNNNRTG 1 cut(s) 58
SalI GTCGAC 1 cut(s) 209
SaqAI TTAA 1 cut(s) 45
SatI GCNGC 2 cut(s) 665, 697
Sau3AI GATC 3 cut(s) 349, 396, 547
Sau96I GGNCC 3 cut(s) 30, 107, 253
SchI GAGTC 2 cut(s) 14, 368
ScrFI CCNGG 2 cut(s) 58, 245
SduI GDGCHC 1 cut(s) 105
SetI ASST 9 cut(s) 120, 145, 190, 219, 412, 488, 508, 521, 698
SfaNI GCATC 1 cut(s) 62
SfcI CTRYAG 1 cut(s) 543
Sfr274I CTCGAG 1 cut(s) 376
SfuI TTCGAA 1 cut(s) 12
SinI GGWCC 1 cut(s) 253
SlaI CTCGAG 1 cut(s) 376
SmiMI CAYNNNNRTG 1 cut(s) 58
SmlI CTYRAG 1 cut(s) 376
SmoI CTYRAG 1 cut(s) 376
SsiI CCGC 1 cut(s) 665
SspI AATATT 1 cut(s) 370
SspMI CTAG 2 cut(s) 336, 458
StyD4I CCNGG 2 cut(s) 56, 243
TaaI ACNGT 3 cut(s) 100, 385, 544
TaiI ACGT 2 cut(s) 120, 190
TaqI TCGA 4 cut(s) 12, 184, 210, 377
TauI GCSGC 1 cut(s) 667
TfiI GAWTC 1 cut(s) 181
Tru1I TTAA 1 cut(s) 45
Tru9I TTAA 1 cut(s) 45
TscAI CASTG 3 cut(s) 55, 103, 484
TseI GCWGC 1 cut(s) 696
TspDTI ATGAA 5 cut(s) 157, 163, 429, 576, 584
TspGWI ACGGA 1 cut(s) 143
TspRI CASTG 3 cut(s) 55, 103, 484
VpaK11BI GGWCC 1 cut(s) 253
XhoI CTCGAG 1 cut(s) 376
XmiI GTMKAC 2 cut(s) 210, 343
XmnI GAANNNNTTC 1 cut(s) 453
XspI CTAG 2 cut(s) 336, 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.