Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0011483

Physical Location & Seq

Genomic Coordinates Forward (+)

63322838 .. 63327783

Gene Structure 4946 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G035610.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2067 bp

ATGCCTTCTATAGACGTTTCCGATTATGTGCTGGAGGCTCCGAAGAAGGAGAAGAAGATGAAAAAGTCGAAAAAGGAGTCCATAACCGACTCTCCGATTAGTGAGAAGAAGAGCAGTAGCAAGAAGGAGTCAAAGCTGAAGAAGCGGAAAGCGCTGGATATGGAGGCCGACGACGAAGACAGGAGCGAGACCAGCTCCGAGCTCGGCGAGCCTGTCAATTTGAAGCCGGACGGCGATTCTTCGGTGGAGAAGAGCAAGAGGAAATCGAAGAAGGCCAAGGTGGAGGAGGAGGAGGAGAAGGTTGAGGTGAAGAAGGATGATCCTAATGCCGTAACCAAGTTTCGGATTTCGGCGCCGTTGAGGGCTAAGCTGAAGGAGAAGGGGATCGAGGCCTTGTTTACGATTCAGGCCATGACGTTCGACACCATTTTGGACGGGGCGGACTTGGTGGGCCGGGCTCGCACCGGTCAGGGTAAAACTCTGGCTTTTGTGTTGCCTATATTGGAGTCGTTAACAAATGGTTCTGCTAAAGAATTTCGAAAGACTGGATACGGCAGAGCACCGACTGTTATTGTTCTTTTACCCACCAGGGAGTTGGCCAAGCAGGTGTTTGCGGACTTTGAATTTTATGGTGGTGCTGTGGGGTTAGCATCGTGTTGTGTATATGGTGGATCTCCTTACGCAGCTCAAGAATATAAGTTGAAGAGAGGGGTTGATGTCATTGTGGGAACACCTGGTCGTATCAAGGATCATATTGAGAGGGGGAATATAGACTTGAGCACTTTGAAGTTTCGGGTCCTTGATGAAGCTGATGAAATGCTGAGGATGGGTTTCGTTGATGACGTTGAACAAATTCTAGGCAAGGTCAAAGATGTAACTAAAGTTCAAACGCTTCTCTTCAGTGCAACTTTACCGTCTTGGGTGCAAAATATTTCCAAAAGGTTTCTTAAATCAGATAAGAAAACTGCAGATCTGGTTGGTAATGAAAAGATGAAGGCCAGCGTCAATGTTAGGCATATTGTTCTTCCTTGCTCTAGTTCAGCTAGAGTAGACGTTATTCCTGATATCATTCGTTGTTATAGCAGTGGAGGTCGCACTATTATTTTCACTGAGACAAAGGATTGTGCTTCTGAGCTTTCTGAGAGGTTGCATGGAGCAAGACCTTTGCACGGGGACATACAGCAAGGTCAGCGGGAGGTCACACTTGCAGGTTTCAGGTCTGGCAAATTCTCGACATTAGTAGCCACAAATGTGGCAGCTCGTGGACTGGATATCAATGATGTTCAATTAATTATTCAGTGTGAACCTCCACGTGATGTAGAAGACTATATCCATCGATCCGGACGCACAGGGAGAGCCGGCAATTCTGGAGTTGCTGTAATGCTTTATGACCCTAGAAAGTCAAACATATCAAAGATCGAAAGAGAATCTGGGGTGAAATTTGAGCATGTTTCTGCTCCTCAGCCAATTGACGTTGCCAAAGCTGCAGGTCTTAGTGCAGCAGAAATGATTACTAATGTCTCTGATAGCGTAATTCCTGCATTCAAGAGTGTTGCTGAGGAGCTTCTAAACACCTCAGGCCTATCTGCAGTAGATCTACTGTCAAAGGCTCTTGCTAAGGCTGCTGGCTACACCGATGTAAAGAAGAGGTCACTTCTGAGTTCTATGGAGAATCATGTCACCATACTGCTTGAAGCTGGAAAGCCCATTTACACAGCATCTTTTGCTTATTCAGCCTTGAGGAGGTTCTTGCCGGAGGAGAAGGTCGAGTCAGTGAAGGGTATGGCGCTAACAACTGATGGAAAGGGTGCAGTGTTTGATGTAGCTGCTGAAGATTTGGATATGTTTCTTACTGGTTCGCAAAATGCAGCTGGTATCAGCATACAGGTCTTGGACTCTTTGCCGAGTTTACAAGAAAAAGAAGCAAGGGGAGGAAGATTTGGCGGTGGTGGCCGTGGTGGTAGTAGGTTTGGTGGAAGAGGAGGTGGCAGGGGTTTCTCTAATGGAAGAAATGATAGGTTTGGTGGCGGCGGTGGCAGAGGCCGCGGCAACTACAACAGATGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017151 GO:0019899 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko01110 map01110

Pfam Domains

Protein Families

DEAD GUCT Helicase_C

Protein Analysis

688

Amino Acids

74.6

Weight (kDa)

9.19

Isoelectric Point (pI)

36.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	134 - 310	2e-44	DEAD/DEAH box helicase
Helicase_C	PF00271	350 - 454	1.9e-27	Helicase conserved C-terminal domain
DD_DDX21-DDX50	PF26142	488 - 540	1.4e-18	DDX21/DDX50 dimerisation domain
GUCT	PF08152	543 - 638	2e-28	GUCT (NUC152) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011483)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G62190
fragaria_vesca	FvH4_7g28410
malus_domestica	MD01G1187800.v1.1 MD07G1259900.v1.1 MD07G1260300.v1.1
prunus_persica	Prupe.2G284200_v2.0.a1 Prupe.2G284200_v2.0.a1 Prupe.2G284200_v2.0.a1
pyrus_communis	pycom07g23330
rosa_chinensis	RchiOBHm_Chr1g0375371
rosa_laevigata	RLG00000026667
rosa_multiflora	Rmu_sc0000554.1_g000041 Rmu_ssc0000106.1_g000003
rosa_roxburghii	Rroxscaffold_4G00282810
rosa_rugosa	Rorug01G0390800
rosa_samantha	Rh1AG401100 Rh1BG363200 Rh1CG376500 Rh1DG394000
rosa_wichuraiana	Rw1G035610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	595
Acc36I	ACCTGC	3 cut(s)	595, 1199, 1478
AccB1I	GGYRCC	1 cut(s)	352
AccI	GTMKAC	1 cut(s)	1050
AccII	CGCG	1 cut(s)	2046
AccIII	TCCGGA	1 cut(s)	1340
AciI	CCGC	9 cut(s)	145, 440, 614, 1192, 1944, 2028, 2031, 2044, 2046
AclWI	GGATC	5 cut(s)	314, 392, 679, 756, 1332
AcoI	YGGCCR	2 cut(s)	597, 1951
AcsI	RAATTY	5 cut(s)	533, 623, 852, 1226, 1439
AcuI	CTGAAG	4 cut(s)	158, 392, 883, 1851
AcvI	CACGTG	1 cut(s)	1313
AcyI	GRCGYC	1 cut(s)	353
AfeI	AGCGCT	1 cut(s)	153
AfiI	CCNNNNNNNGG	3 cut(s)	342, 1169, 1743
AgeI	ACCGGT	1 cut(s)	464
AgsI	TTSAA	9 cut(s)	223, 623, 703, 787, 848, 887, 1286, 1546, 1694
AjnI	CCWGG	2 cut(s)	587, 733
AleI	CACNNNNGTG	1 cut(s)	1250
Alw21I	GWGCWC	3 cut(s)	204, 562, 782
Alw26I	GTCTC	3 cut(s)	182, 1106, 1525
AlwI	GGATC	5 cut(s)	314, 392, 679, 756, 1332
Aor13HI	TCCGGA	1 cut(s)	1340
Aor51HI	AGCGCT	1 cut(s)	153
ApeKI	GCWGC	7 cut(s)	683, 1256, 1484, 1499, 1622, 1826, 1868
ApoI	RAATTY	5 cut(s)	533, 623, 852, 1226, 1439
ArsI	GACNNNNNNTTYG	2 cut(s)	62, 94
AseI	ATTAAT	1 cut(s)	1289
AsiGI	ACCGGT	1 cut(s)	464
Asp700I	GAANNNNTTC	1 cut(s)	852
AspLEI	GCGC	3 cut(s)	154, 355, 1789
AspS9I	GGNCC	2 cut(s)	451, 796
AsuC2I	CCSGG	1 cut(s)	455
AsuHPI	GGTGA	3 cut(s)	319, 1447, 1672
AsuII	TTCGAA	1 cut(s)	538
AvaII	GGWCC	1 cut(s)	796
AxyI	CCTNAGG	1 cut(s)	1576
BalI	TGGCCA	1 cut(s)	599
BanI	GGYRCC	1 cut(s)	352
BanII	GRGCYC	2 cut(s)	204, 460
BauI	CACGAG	1 cut(s)	1260
BbrPI	CACGTG	1 cut(s)	1313
BbsI	GAAGAC	2 cut(s)	183, 1329
Bbv12I	GWGCWC	3 cut(s)	204, 562, 782
BbvCI	CCTCAGC	3 cut(s)	821, 1461, 1557
BbvI	GCAGC	7 cut(s)	695, 1268, 1471, 1511, 1609, 1813, 1880
BccI	CCATC	4 cut(s)	820, 1341, 1793, 2055
BceAI	ACGGC	5 cut(s)	247, 314, 340, 568, 1938
BciT130I	CCWGG	2 cut(s)	589, 735
BciVI	GTATCC	1 cut(s)	542
BcnI	CCSGG	1 cut(s)	455
BcoDI	GTCTC	3 cut(s)	182, 1106, 1525
BfaI	CTAG	4 cut(s)	857, 1035, 1044, 1395
BfmI	CTRYAG	4 cut(s)	9, 966, 1485, 1587
BfoI	RGCGCY	3 cut(s)	155, 356, 1790
BfuAI	ACCTGC	3 cut(s)	595, 1199, 1478
BfuI	GTATCC	1 cut(s)	542
BglII	AGATCT	2 cut(s)	970, 1594
BlpI	GCTNAGC	1 cut(s)	366
Bme1390I	CCNGG	3 cut(s)	455, 589, 735
Bme18I	GGWCC	1 cut(s)	796
BmgT120I	GGNCC	2 cut(s)	451, 796
BmiI	GGNNCC	3 cut(s)	39, 354, 797
BmrFI	CCNGG	3 cut(s)	455, 589, 735
BmsI	GCATC	2 cut(s)	659, 1727
BpiI	GAAGAC	2 cut(s)	183, 1329
BplI	GAGNNNNNCTC	2 cut(s)	179, 211
BpmI	CTGGAG	2 cut(s)	53, 1389
Bpu10I	CCTNAGC	4 cut(s)	821, 1461, 1557, 1617
Bpu1102I	GCTNAGC	1 cut(s)	366
Bpu14I	TTCGAA	1 cut(s)	538
BpuEI	CTTGAG	3 cut(s)	672, 796, 1759
BpuMI	CCSGG	1 cut(s)	455
Bsa29I	ATCGAT	1 cut(s)	1336
BsaAI	YACGTR	1 cut(s)	1313
BsaHI	GRCGYC	1 cut(s)	353
BsaI	GGTCTC	1 cut(s)	182
BsaJI	CCNNGG	4 cut(s)	276, 588, 1954, 2044
BsaWI	WCCGGW	2 cut(s)	464, 1340
BsaXI	ACNNNNNCTCC	4 cut(s)	76, 106, 1362, 1392
Bsc4I	CCNNNNNNNGG	3 cut(s)	342, 1169, 1743
Bse118I	RCCGGY	2 cut(s)	464, 1358
Bse1I	ACTGG	3 cut(s)	550, 1272, 1858
Bse21I	CCTNAGG	1 cut(s)	1576
BseAI	TCCGGA	1 cut(s)	1340
BseBI	CCWGG	2 cut(s)	589, 735
BseCI	ATCGAT	1 cut(s)	1336
BseDI	CCNNGG	4 cut(s)	276, 588, 1954, 2044
BseGI	GGATG	2 cut(s)	322, 831
BseLI	CCNNNNNNNGG	3 cut(s)	342, 1169, 1743
BseMII	CTCAG	8 cut(s)	812, 1101, 1122, 1131, 1475, 1548, 1590, 1649
BseNI	ACTGG	3 cut(s)	550, 1272, 1858
BseRI	GAGGAG	9 cut(s)	299, 302, 305, 308, 1449, 1574, 1756, 1772, 1995
BseXI	GCAGC	7 cut(s)	695, 1268, 1471, 1511, 1609, 1813, 1880
BsgI	GTGCAG	2 cut(s)	1518, 1830
Bsh1236I	CGCG	1 cut(s)	2046
BshNI	GGYRCC	1 cut(s)	352
BshTI	ACCGGT	1 cut(s)	464
BshVI	ATCGAT	1 cut(s)	1336
BsiHKAI	GWGCWC	3 cut(s)	204, 562, 782
BsiSI	CCGG	6 cut(s)	227, 454, 465, 1341, 1359, 1754
BslFI	GGGAC	1 cut(s)	1187
BslI	CCNNNNNNNGG	3 cut(s)	342, 1169, 1743
BsmAI	GTCTC	3 cut(s)	182, 1106, 1525
BsmFI	GGGAC	1 cut(s)	1187
BsmI	GAATGC	1 cut(s)	1541
Bso31I	GGTCTC	1 cut(s)	182
Bsp119I	TTCGAA	1 cut(s)	538
Bsp1286I	GDGCHC	4 cut(s)	204, 460, 562, 782
Bsp13I	TCCGGA	1 cut(s)	1340
Bsp143I	GATC	8 cut(s)	319, 384, 671, 748, 970, 1337, 1416, 1594
Bsp1720I	GCTNAGC	1 cut(s)	366
BspACI	CCGC	9 cut(s)	145, 440, 614, 1192, 1944, 2028, 2031, 2044, 2046
BspCNI	CTCAG	8 cut(s)	813, 1102, 1123, 1132, 1474, 1549, 1589, 1650
BspDI	ATCGAT	1 cut(s)	1336
BspEI	TCCGGA	1 cut(s)	1340
BspFNI	CGCG	1 cut(s)	2046
BspLI	GGNNCC	3 cut(s)	39, 354, 797
BspMAI	CTGCAG	3 cut(s)	970, 1489, 1591
BspMI	ACCTGC	3 cut(s)	595, 1199, 1478
BspPI	GGATC	5 cut(s)	314, 392, 679, 756, 1332
BspQI	GCTCTTC	2 cut(s)	104, 245
BspT104I	TTCGAA	1 cut(s)	538
BspT107I	GGYRCC	1 cut(s)	352
BspTNI	GGTCTC	1 cut(s)	182
BsrFI	RCCGGY	2 cut(s)	464, 1358
BsrI	ACTGG	3 cut(s)	550, 1272, 1858
BssAI	RCCGGY	2 cut(s)	464, 1358
BssECI	CCNNGG	4 cut(s)	276, 588, 1954, 2044
BssMI	GATC	8 cut(s)	319, 384, 671, 748, 970, 1337, 1416, 1594
BssNI	GRCGYC	1 cut(s)	353
BssSI	CACGAG	1 cut(s)	1260
BssT1I	CCWWGG	1 cut(s)	276
Bst2BI	CACGAG	1 cut(s)	1260
Bst2UI	CCWGG	2 cut(s)	589, 735
Bst4CI	ACNGT	3 cut(s)	568, 915, 1602
Bst6I	CTCTTC	6 cut(s)	104, 245, 698, 902, 1640, 1972
BstACI	GRCGYC	1 cut(s)	353
BstAPI	GCANNNNNTGC	1 cut(s)	1724
BstBAI	YACGTR	1 cut(s)	1313
BstBI	TTCGAA	1 cut(s)	538
BstC8I	GCNNGC	5 cut(s)	209, 460, 1000, 1360, 1627
BstDSI	CCRYGG	2 cut(s)	1954, 2044
BstENI	CCTNNNNNAGG	1 cut(s)	1741
BstF5I	GGATG	2 cut(s)	322, 831
BstFNI	CGCG	1 cut(s)	2046
BstH2I	RGCGCY	3 cut(s)	155, 356, 1790
BstHHI	GCGC	3 cut(s)	154, 355, 1789
BstKTI	GATC	8 cut(s)	322, 387, 674, 751, 973, 1340, 1419, 1597
BstMAI	GTCTC	3 cut(s)	182, 1106, 1525
BstMBI	GATC	8 cut(s)	319, 384, 671, 748, 970, 1337, 1416, 1594
BstNI	CCWGG	2 cut(s)	589, 735
BstNSI	RCATGY	1 cut(s)	1451
BstSCI	CCNGG	3 cut(s)	453, 587, 733
BstSFI	CTRYAG	4 cut(s)	9, 966, 1485, 1587
BstUI	CGCG	1 cut(s)	2046
BstV1I	GCAGC	7 cut(s)	695, 1268, 1471, 1511, 1609, 1813, 1880
BstV2I	GAAGAC	2 cut(s)	183, 1329
BstX2I	RGATCY	3 cut(s)	671, 970, 1594
BstXI	CCANNNNNNTGG	2 cut(s)	595, 1252
BstYI	RGATCY	3 cut(s)	671, 970, 1594
Bsu15I	ATCGAT	1 cut(s)	1336
Bsu36I	CCTNAGG	1 cut(s)	1576
BsuI	GTATCC	1 cut(s)	542
BsuTUI	ATCGAT	1 cut(s)	1336
BtgI	CCRYGG	2 cut(s)	1954, 2044
BtsCI	GGATG	2 cut(s)	322, 831
BtsI	GCAGTG	2 cut(s)	1090, 1818
BtsIMutI	CAGTG	6 cut(s)	907, 1090, 1107, 1304, 1779, 1818
BveI	ACCTGC	3 cut(s)	595, 1199, 1478
Cac8I	GCNNGC	5 cut(s)	209, 460, 1000, 1360, 1627
CfoI	GCGC	3 cut(s)	154, 355, 1789
Cfr10I	RCCGGY	2 cut(s)	464, 1358
Cfr13I	GGNCC	2 cut(s)	451, 796
Cfr42I	CCGCGG	1 cut(s)	2047
ClaI	ATCGAT	1 cut(s)	1336
CseI	GACGC	2 cut(s)	991, 1353
CsiI	ACCWGGT	1 cut(s)	733
CspAI	ACCGGT	1 cut(s)	464
CviAII	CATG	4 cut(s)	412, 1151, 1448, 1676
DinI	GGCGCC	1 cut(s)	354
DpnI	GATC	8 cut(s)	321, 386, 673, 750, 972, 1339, 1418, 1596
DpnII	GATC	8 cut(s)	319, 384, 671, 748, 970, 1337, 1416, 1594
EaeI	YGGCCR	2 cut(s)	597, 1951
Eam1104I	CTCTTC	6 cut(s)	104, 245, 698, 902, 1640, 1972
EarI	CTCTTC	6 cut(s)	104, 245, 698, 902, 1640, 1972
EciI	GGCGGA	1 cut(s)	455
Ecl136II	GAGCTC	1 cut(s)	202
Eco130I	CCWWGG	1 cut(s)	276
Eco147I	AGGCCT	2 cut(s)	392, 1581
Eco24I	GRGCYC	2 cut(s)	204, 460
Eco31I	GGTCTC	1 cut(s)	182
Eco32I	GATATC	2 cut(s)	1066, 1273
Eco47I	GGWCC	1 cut(s)	796
Eco47III	AGCGCT	1 cut(s)	153
Eco53kI	GAGCTC	1 cut(s)	202
Eco57I	CTGAAG	4 cut(s)	158, 392, 883, 1851
Eco72I	CACGTG	1 cut(s)	1313
Eco81I	CCTNAGG	1 cut(s)	1576
EcoICRI	GAGCTC	1 cut(s)	202
EcoNI	CCTNNNNNAGG	1 cut(s)	1741
EcoO109I	RGGNCCY	1 cut(s)	796
EcoRII	CCWGG	2 cut(s)	587, 733
EcoRV	GATATC	2 cut(s)	1066, 1273
EcoT14I	CCWWGG	1 cut(s)	276
EcoT38I	GRGCYC	2 cut(s)	204, 460
EgeI	GGCGCC	1 cut(s)	354
EheI	GGCGCC	1 cut(s)	354
ErhI	CCWWGG	1 cut(s)	276
FaeI	CATG	4 cut(s)	415, 1154, 1451, 1679
FaqI	GGGAC	1 cut(s)	1187
FatI	CATG	4 cut(s)	411, 1150, 1447, 1675
FauI	CCCGC	1 cut(s)	1185
FblI	GTMKAC	1 cut(s)	1050
FokI	GGATG	2 cut(s)	329, 838
FriOI	GRGCYC	2 cut(s)	204, 460
FspBI	CTAG	4 cut(s)	857, 1035, 1044, 1395
GlaI	GCGC	3 cut(s)	153, 354, 1788
GsuI	CTGGAG	2 cut(s)	53, 1389
HaeII	RGCGCY	3 cut(s)	155, 356, 1790
HapII	CCGG	6 cut(s)	227, 454, 465, 1341, 1359, 1754
HgaI	GACGC	2 cut(s)	991, 1353
HhaI	GCGC	3 cut(s)	154, 355, 1789
Hin1I	GRCGYC	1 cut(s)	353
Hin1II	CATG	4 cut(s)	415, 1154, 1451, 1679
Hin6I	GCGC	3 cut(s)	152, 353, 1787
HinP1I	GCGC	3 cut(s)	152, 353, 1787
HincII	GTYRAC	1 cut(s)	513
HindII	GTYRAC	1 cut(s)	513
HpaI	GTTAAC	1 cut(s)	513
HpaII	CCGG	6 cut(s)	227, 454, 465, 1341, 1359, 1754
HphI	GGTGA	3 cut(s)	319, 1447, 1672
Hpy166II	GTNNAC	6 cut(s)	399, 513, 1051, 1265, 1304, 1910
Hpy188III	TCNNGA	6 cut(s)	689, 1061, 1231, 1341, 1368, 1546
Hpy8I	GTNNAC	6 cut(s)	399, 513, 1051, 1265, 1304, 1910
Hpy99I	CGWCG	2 cut(s)	173, 176
HpyCH4III	ACNGT	3 cut(s)	568, 915, 1602
HpyCH4IV	ACGT	6 cut(s)	15, 416, 843, 1053, 1312, 1473
HpySE526I	ACGT	6 cut(s)	15, 416, 843, 1053, 1312, 1473
Hsp92I	GRCGYC	1 cut(s)	353
Hsp92II	CATG	4 cut(s)	415, 1154, 1451, 1679
HspAI	GCGC	3 cut(s)	152, 353, 1787
KasI	GGCGCC	1 cut(s)	352
Kpn2I	TCCGGA	1 cut(s)	1340
KroI	GCCGGC	1 cut(s)	1358
KroNI	GCCGGC	1 cut(s)	1360
KspAI	GTTAAC	1 cut(s)	513
KspI	CCGCGG	1 cut(s)	2047
Kzo9I	GATC	8 cut(s)	319, 384, 671, 748, 970, 1337, 1416, 1594
LguI	GCTCTTC	2 cut(s)	104, 245
LmnI	GCTCC	6 cut(s)	43, 183, 200, 1154, 1462, 1561
Lsp1109I	GCAGC	7 cut(s)	695, 1268, 1471, 1511, 1609, 1813, 1880
LweI	GCATC	2 cut(s)	659, 1727
MabI	ACCWGGT	1 cut(s)	733
MaeI	CTAG	4 cut(s)	857, 1035, 1044, 1395
MaeII	ACGT	6 cut(s)	15, 416, 843, 1053, 1312, 1473
MaeIII	GTNAC	5 cut(s)	331, 874, 1198, 1650, 1678
MalI	GATC	8 cut(s)	321, 386, 673, 750, 972, 1339, 1418, 1596
MboI	GATC	8 cut(s)	319, 384, 671, 748, 970, 1337, 1416, 1594
MfeI	CAATTG	1 cut(s)	1467
MflI	RGATCY	3 cut(s)	671, 970, 1594
MhlI	GDGCHC	4 cut(s)	204, 460, 562, 782
MlsI	TGGCCA	1 cut(s)	599
MluNI	TGGCCA	1 cut(s)	599
Mly113I	GGCGCC	1 cut(s)	353
MlyI	GAGTC	6 cut(s)	83, 86, 137, 515, 1778, 1889
Mox20I	TGGCCA	1 cut(s)	599
MroI	TCCGGA	1 cut(s)	1340
MroNI	GCCGGC	1 cut(s)	1358
MroXI	GAANNNNTTC	1 cut(s)	852
MscI	TGGCCA	1 cut(s)	599
MseI	TTAA	3 cut(s)	512, 948, 1289
MslI	CAYNNNNRTG	1 cut(s)	1250
Msp20I	TGGCCA	1 cut(s)	599
MspA1I	CMGCKG	3 cut(s)	1192, 1871, 2046
MspI	CCGG	6 cut(s)	227, 454, 465, 1341, 1359, 1754
MspR9I	CCNGG	3 cut(s)	455, 589, 735
MunI	CAATTG	1 cut(s)	1467
Mva1269I	GAATGC	1 cut(s)	1541
MvaI	CCWGG	2 cut(s)	589, 735
MvnI	CGCG	1 cut(s)	2046
NaeI	GCCGGC	1 cut(s)	1360
NarI	GGCGCC	1 cut(s)	353
NciI	CCSGG	1 cut(s)	455
NdeII	GATC	8 cut(s)	319, 384, 671, 748, 970, 1337, 1416, 1594
NgoMIV	GCCGGC	1 cut(s)	1358
NlaIII	CATG	4 cut(s)	415, 1154, 1451, 1679
NlaIV	GGNNCC	3 cut(s)	39, 354, 797
NmeAIII	GCCGAG	2 cut(s)	183, 1929
NmuCI	GTSAC	3 cut(s)	1198, 1650, 1678
NspI	RCATGY	1 cut(s)	1451
NspV	TTCGAA	1 cut(s)	538
OliI	CACNNNNGTG	1 cut(s)	1250
PaqCI	CACCTGC	1 cut(s)	595
PceI	AGGCCT	2 cut(s)	392, 1581
PciSI	GCTCTTC	2 cut(s)	104, 245
PcsI	WCGNNNNNNNCGW	1 cut(s)	840
PctI	GAATGC	1 cut(s)	1541
PdiI	GCCGGC	1 cut(s)	1360
PdmI	GAANNNNTTC	1 cut(s)	852
PfeI	GAWTC	4 cut(s)	236, 403, 1427, 1672
PinAI	ACCGGT	1 cut(s)	464
PleI	GAGTC	6 cut(s)	83, 85, 136, 514, 1777, 1889
PluTI	GGCGCC	1 cut(s)	356
PmaCI	CACGTG	1 cut(s)	1313
PmlI	CACGTG	1 cut(s)	1313
PpsI	GAGTC	6 cut(s)	83, 85, 136, 514, 1777, 1889
Ppu21I	YACGTR	1 cut(s)	1313
PpuMI	RGGWCCY	1 cut(s)	796
PshBI	ATTAAT	1 cut(s)	1289
Psp124BI	GAGCTC	1 cut(s)	204
Psp5II	RGGWCCY	1 cut(s)	796
Psp6I	CCWGG	2 cut(s)	587, 733
PspCI	CACGTG	1 cut(s)	1313
PspGI	CCWGG	2 cut(s)	587, 733
PspN4I	GGNNCC	3 cut(s)	39, 354, 797
PspPI	GGNCC	2 cut(s)	451, 796
PspPPI	RGGWCCY	1 cut(s)	796
PsrI	GAACNNNNNNTAC	2 cut(s)	867, 899
PstI	CTGCAG	3 cut(s)	970, 1489, 1591
PsuI	RGATCY	3 cut(s)	671, 970, 1594
PvuII	CAGCTG	1 cut(s)	1871
RseI	CAYNNNNRTG	1 cut(s)	1250
SacI	GAGCTC	1 cut(s)	204
SacII	CCGCGG	1 cut(s)	2047
SapI	GCTCTTC	2 cut(s)	104, 245
SaqAI	TTAA	3 cut(s)	512, 948, 1289
Sau3AI	GATC	8 cut(s)	319, 384, 671, 748, 970, 1337, 1416, 1594
Sau96I	GGNCC	2 cut(s)	451, 796
SchI	GAGTC	6 cut(s)	83, 86, 137, 515, 1778, 1889
ScrFI	CCNGG	3 cut(s)	455, 589, 735
SduI	GDGCHC	4 cut(s)	204, 460, 562, 782
SexAI	ACCWGGT	1 cut(s)	733
SfaNI	GCATC	2 cut(s)	659, 1727
SfcI	CTRYAG	4 cut(s)	9, 966, 1485, 1587
SfoI	GGCGCC	1 cut(s)	354
Sfr303I	CCGCGG	1 cut(s)	2047
SfuI	TTCGAA	1 cut(s)	538
SgrBI	CCGCGG	1 cut(s)	2047
SinI	GGWCC	1 cut(s)	796
SmiMI	CAYNNNNRTG	1 cut(s)	1250
SmlI	CTYRAG	3 cut(s)	687, 775, 1738
SmoI	CTYRAG	3 cut(s)	687, 775, 1738
SseBI	AGGCCT	2 cut(s)	392, 1581
SsiI	CCGC	9 cut(s)	145, 440, 614, 1192, 1944, 2028, 2031, 2044, 2046
SspDI	GGCGCC	1 cut(s)	352
SspI	AATATT	1 cut(s)	931
SspMI	CTAG	4 cut(s)	857, 1035, 1044, 1395
SstI	GAGCTC	1 cut(s)	204
StuI	AGGCCT	2 cut(s)	392, 1581
StyD4I	CCNGG	3 cut(s)	453, 587, 733
StyI	CCWWGG	1 cut(s)	276
TaaI	ACNGT	3 cut(s)	568, 915, 1602
TaiI	ACGT	6 cut(s)	18, 419, 846, 1056, 1315, 1476
TaqI	TCGA	9 cut(s)	68, 266, 387, 420, 538, 1232, 1336, 1419, 1767
TauI	GCSGC	3 cut(s)	2031, 2046, 2049
TfiI	GAWTC	4 cut(s)	236, 403, 1427, 1672
Tru1I	TTAA	3 cut(s)	512, 948, 1289
Tru9I	TTAA	3 cut(s)	512, 948, 1289
TscAI	CASTG	6 cut(s)	907, 1090, 1114, 1304, 1779, 1818
TseFI	GTSAC	3 cut(s)	1198, 1650, 1678
TseI	GCWGC	7 cut(s)	683, 1256, 1484, 1499, 1622, 1826, 1868
Tsp45I	GTSAC	3 cut(s)	1198, 1650, 1678
TspDTI	ATGAA	5 cut(s)	74, 819, 828, 999, 1007
TspRI	CASTG	6 cut(s)	907, 1090, 1114, 1304, 1779, 1818
VpaK11BI	GGWCC	1 cut(s)	796
VspI	ATTAAT	1 cut(s)	1289
XagI	CCTNNNNNAGG	1 cut(s)	1741
XapI	RAATTY	5 cut(s)	533, 623, 852, 1226, 1439
XceI	RCATGY	1 cut(s)	1451
XcmI	CCANNNNNNNNNTGG	1 cut(s)	592
XmiI	GTMKAC	1 cut(s)	1050
XmnI	GAANNNNTTC	1 cut(s)	852
XspI	CTAG	4 cut(s)	857, 1035, 1044, 1395

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.