Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1A

Orthogroup ID

OG0011483

Physical Location & Seq

Genomic Coordinates Forward (+)

62771074 .. 62775418

Gene Structure 4345 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1AG401100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2076 bp

ATGCCTTCTATAGACGTTTCCGATTATGTGCTGGAGGCTCCGAAGAAGGAGAAGAAGATGAAAAAGTCGAAAAAGGAGTCCATAACCGACTCTCCGATTAGTGAGAAGAAGAGCAGTAGCAAGAAGGAGTCAAAGCTGAAGAAGCGGAAAGCGCTGGATATGGAGGCCGACGACGAAGACAGGAGCGAGACCAGCTCCGAGCTCGGCGAGCCTGTCAATTTGAAGCCCAACGGCGATTCTTCGGTGGAGAAGAGCAAGAGGAAATCGAAGAAGGCCAAGGTGGAGGAGGAGGAGGAGGAAGAGGAGAAGGTTGAGGTGAAGAAGGATGATCCTAATGCCGTAACCAAGTTTCGGATTTCGGCGCCGTTGAGGGCTAAGCTGAAGGAGAAGGGGATCGAGGCCTTGTTTACGATTCAGGCCATGACGTTCGACACCATTTTGGACGGGGCGGACTTGGTGGGCCGGGCTCGCACCGGTCAGGGTAAAACTCTGGCTTTTGTGTTGCCTATCTTGGAGTCGTTAACAAATGGTTCTGCTAAAGAATTTCGAAAGACTGGATACGGCAGGGCACCGACTGTTATTGTTCTTTTACCCACCAGGGAGTTGGCCAAGCAGGTGTTTGCGGACTTTGAATTTTATGGTGGTGCTGTGGGGTTAGCATCGTGTTGTGTATATGGTGGATCTCCTTACGCAGCTCAAGAATATAAGTTGAAGAGAGGGGTTGATGTCATTGTGGGAACGCCTGGTCGTATCAAGGATCATATTGAGAGGGGGAATATAGACTTGAGCACTTTGAAGTTTCGGGTCCTTGATGAAGCTGATGAAATGCTGAGGATGGGTTTTGTTGATGACGTTGAACAAATTCTAGGCAAGGTCAAAGATGTAACTAAAGTTCAAACGCTTCTCTTCAGTGCAACTTTACCCTCTTGGGTGCAAAATATTTCCAAAAGGTTTCTTAAATCAGATAAGAAAACTGCAGATCTGGTTGGTAATGAAAAGATGAAGGCCAGCGTCAATGTTAGGCATATTGTTCTTCCTTGCTCTAGTTCAGCTAGAGTAGACGTTATTCCTGATATCATTCGTTGTTATAGCAGTGGAGGTCGCACTATTATTTTCACTGAGACAAAGGATTGTGCTTCTGAGCTTTCTGAGAGGTTGCATGGAGCAAGACCTTTGCACGGGGACATACAGCAAGGTCAGCGGGAGGTCACACTTGCAGGTTTCAGGTCTGGCAAATTCTCGACATTAGTAGCCACAAATGTGGCAGCTCGTGGACTGGATATCAATGATGTTCAATTAATTATTCAGTGTGAACCTCCACGTGATGTAGAAGACTATATCCATCGATCCGGACGCACAGGGAGAGCCGGCAATTCTGGAGTTGCTGTAATGCTTTATGACCCTAGAAAGTCAAACATATCAAAGATCGAAAGAGAATCTGGGGTGAAATTTGAGCATGTTTCTGCTCCTCAGCCAATTGACGTTGCCAAAGCTGCAGGTCTTAGTGCAGCAGAAATGATTACTAATGTCTCTGATAGCGTAATTCCTGCATTCAAGAGTGTTGCTGAGGAGCTTCTAAACACCTCAGGCCTATCTGCAGTAGATCTACTGTCAAAGGCTCTTGCTAAGGCTGCTGGCTACACCGATGTAAAGAAGAGGTCACTTCTGAGTTCTATGGAGAATCATGTCACCATACTGCTTGAAGCTGGAAAGCCCATTTACACAGCATCTTTTGCTTATTCAGCCTTGAGGAGGTTCTTGCCGGAGGAGAAGGTCGAGTCAGTGAAGGGTATGGCGCTAACAACTGATGGAAAGGGTGCAGTGTTTGATGTAGCTGCTGAAGATTTGGATATGTTTCTTACTGGTTCGCAAAATGCAGCTGGTATCAGCATACAGGTCTTGGACTCTTTGCCGAGTTTACAAGAAAAAGAAGCAAGGGGAGGAAGATTTGGCGGTGGTGGCCGTGGTGGTAGTAGGTTTGGTGGAAGAGGAGGTGGCAGGGGTTTCTCTAATGGAAGAAATGATAGGTTTGGTGCCGGCGGTGGCAGAGGCCGCGGCAACTACAACAGATGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017151 GO:0019899 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko01110 map01110

Pfam Domains

Protein Families

DEAD GUCT Helicase_C

Protein Analysis

691

Amino Acids

75.0

Weight (kDa)

9.11

Isoelectric Point (pI)

37.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	137 - 313	2e-44	DEAD/DEAH box helicase
Helicase_C	PF00271	353 - 457	1.9e-27	Helicase conserved C-terminal domain
DD_DDX21-DDX50	PF26142	491 - 543	1.4e-18	DDX21/DDX50 dimerisation domain
GUCT	PF08152	546 - 641	2e-28	GUCT (NUC152) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0011483)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G62190
fragaria_vesca	FvH4_7g28410
malus_domestica	MD01G1187800.v1.1 MD07G1259900.v1.1 MD07G1260300.v1.1
prunus_persica	Prupe.2G284200_v2.0.a1 Prupe.2G284200_v2.0.a1 Prupe.2G284200_v2.0.a1
pyrus_communis	pycom07g23330
rosa_chinensis	RchiOBHm_Chr1g0375371
rosa_laevigata	RLG00000026667
rosa_multiflora	Rmu_sc0000554.1_g000041 Rmu_ssc0000106.1_g000003
rosa_roxburghii	Rroxscaffold_4G00282810
rosa_rugosa	Rorug01G0390800
rosa_samantha	Rh1AG401100 Rh1BG363200 Rh1CG376500 Rh1DG394000
rosa_wichuraiana	Rw1G035610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	604
Acc36I	ACCTGC	3 cut(s)	604, 1208, 1487
AccB1I	GGYRCC	3 cut(s)	361, 568, 2033
AccI	GTMKAC	1 cut(s)	1059
AccII	CGCG	1 cut(s)	2055
AccIII	TCCGGA	1 cut(s)	1349
AciI	CCGC	8 cut(s)	145, 449, 623, 1201, 1953, 2040, 2053, 2055
AclWI	GGATC	5 cut(s)	323, 401, 688, 765, 1341
AcoI	YGGCCR	2 cut(s)	606, 1960
AcsI	RAATTY	5 cut(s)	542, 632, 861, 1235, 1448
AcuI	CTGAAG	4 cut(s)	158, 401, 892, 1860
AcvI	CACGTG	1 cut(s)	1322
AcyI	GRCGYC	1 cut(s)	362
AfeI	AGCGCT	1 cut(s)	153
AfiI	CCNNNNNNNGG	3 cut(s)	351, 1178, 1752
AgeI	ACCGGT	1 cut(s)	473
AgsI	TTSAA	9 cut(s)	223, 632, 712, 796, 857, 896, 1295, 1555, 1703
AjnI	CCWGG	2 cut(s)	596, 742
AleI	CACNNNNGTG	1 cut(s)	1259
Alw21I	GWGCWC	2 cut(s)	204, 791
Alw26I	GTCTC	3 cut(s)	182, 1115, 1534
AlwI	GGATC	5 cut(s)	323, 401, 688, 765, 1341
Aor13HI	TCCGGA	1 cut(s)	1349
Aor51HI	AGCGCT	1 cut(s)	153
ApeKI	GCWGC	7 cut(s)	692, 1265, 1493, 1508, 1631, 1835, 1877
ApoI	RAATTY	5 cut(s)	542, 632, 861, 1235, 1448
ArsI	GACNNNNNNTTYG	2 cut(s)	62, 94
AseI	ATTAAT	1 cut(s)	1298
AsiGI	ACCGGT	1 cut(s)	473
Asp700I	GAANNNNTTC	1 cut(s)	861
AspLEI	GCGC	3 cut(s)	154, 364, 1798
AspS9I	GGNCC	2 cut(s)	460, 805
AsuC2I	CCSGG	1 cut(s)	464
AsuHPI	GGTGA	3 cut(s)	328, 1456, 1681
AsuII	TTCGAA	1 cut(s)	547
AvaII	GGWCC	1 cut(s)	805
AxyI	CCTNAGG	1 cut(s)	1585
BaeGI	GKGCMC	1 cut(s)	571
BalI	TGGCCA	1 cut(s)	608
BanI	GGYRCC	3 cut(s)	361, 568, 2033
BanII	GRGCYC	2 cut(s)	204, 469
BauI	CACGAG	1 cut(s)	1269
BbrPI	CACGTG	1 cut(s)	1322
BbsI	GAAGAC	2 cut(s)	183, 1338
Bbv12I	GWGCWC	2 cut(s)	204, 791
BbvCI	CCTCAGC	3 cut(s)	830, 1470, 1566
BbvI	GCAGC	7 cut(s)	704, 1277, 1480, 1520, 1618, 1822, 1889
BccI	CCATC	4 cut(s)	829, 1350, 1802, 2064
BceAI	ACGGC	5 cut(s)	247, 323, 349, 577, 1947
BciT130I	CCWGG	2 cut(s)	598, 744
BciVI	GTATCC	1 cut(s)	551
BcnI	CCSGG	1 cut(s)	464
BcoDI	GTCTC	3 cut(s)	182, 1115, 1534
BfaI	CTAG	4 cut(s)	866, 1044, 1053, 1404
BfmI	CTRYAG	4 cut(s)	9, 975, 1494, 1596
BfoI	RGCGCY	3 cut(s)	155, 365, 1799
BfuAI	ACCTGC	3 cut(s)	604, 1208, 1487
BfuI	GTATCC	1 cut(s)	551
BglII	AGATCT	2 cut(s)	979, 1603
BisI	GCNGC	9 cut(s)	693, 1266, 1494, 1509, 1632, 1836, 1878, 2053, 2056
BlpI	GCTNAGC	1 cut(s)	375
BlsI	GCNGC	9 cut(s)	694, 1267, 1495, 1510, 1633, 1837, 1879, 2054, 2057
Bme1390I	CCNGG	3 cut(s)	464, 598, 744
Bme18I	GGWCC	1 cut(s)	805
BmgT120I	GGNCC	2 cut(s)	460, 805
BmiI	GGNNCC	5 cut(s)	39, 363, 570, 806, 2035
BmrFI	CCNGG	3 cut(s)	464, 598, 744
BmsI	GCATC	2 cut(s)	668, 1736
BpiI	GAAGAC	2 cut(s)	183, 1338
BplI	GAGNNNNNCTC	2 cut(s)	179, 211
BpmI	CTGGAG	2 cut(s)	53, 1398
Bpu10I	CCTNAGC	4 cut(s)	830, 1470, 1566, 1626
Bpu1102I	GCTNAGC	1 cut(s)	375
Bpu14I	TTCGAA	1 cut(s)	547
BpuEI	CTTGAG	3 cut(s)	681, 805, 1768
BpuMI	CCSGG	1 cut(s)	464
Bsa29I	ATCGAT	1 cut(s)	1345
BsaAI	YACGTR	1 cut(s)	1322
BsaHI	GRCGYC	1 cut(s)	362
BsaI	GGTCTC	1 cut(s)	182
BsaJI	CCNNGG	4 cut(s)	276, 597, 1963, 2053
BsaWI	WCCGGW	2 cut(s)	473, 1349
BsaXI	ACNNNNNCTCC	4 cut(s)	76, 106, 1371, 1401
Bsc4I	CCNNNNNNNGG	3 cut(s)	351, 1178, 1752
Bse118I	RCCGGY	3 cut(s)	473, 1367, 2036
Bse1I	ACTGG	3 cut(s)	559, 1281, 1867
Bse21I	CCTNAGG	1 cut(s)	1585
BseAI	TCCGGA	1 cut(s)	1349
BseBI	CCWGG	2 cut(s)	598, 744
BseCI	ATCGAT	1 cut(s)	1345
BseDI	CCNNGG	4 cut(s)	276, 597, 1963, 2053
BseGI	GGATG	2 cut(s)	331, 840
BseLI	CCNNNNNNNGG	3 cut(s)	351, 1178, 1752
BseMII	CTCAG	8 cut(s)	821, 1110, 1131, 1140, 1484, 1557, 1599, 1658
BseNI	ACTGG	3 cut(s)	559, 1281, 1867
BseSI	GKGCMC	1 cut(s)	571
BseXI	GCAGC	7 cut(s)	704, 1277, 1480, 1520, 1618, 1822, 1889
BsgI	GTGCAG	2 cut(s)	1527, 1839
Bsh1236I	CGCG	1 cut(s)	2055
BshNI	GGYRCC	3 cut(s)	361, 568, 2033
BshTI	ACCGGT	1 cut(s)	473
BshVI	ATCGAT	1 cut(s)	1345
BsiHKAI	GWGCWC	2 cut(s)	204, 791
BsiSI	CCGG	6 cut(s)	463, 474, 1350, 1368, 1763, 2037
BslFI	GGGAC	1 cut(s)	1196
BslI	CCNNNNNNNGG	3 cut(s)	351, 1178, 1752
BsmAI	GTCTC	3 cut(s)	182, 1115, 1534
BsmFI	GGGAC	1 cut(s)	1196
BsmI	GAATGC	1 cut(s)	1550
Bso31I	GGTCTC	1 cut(s)	182
Bsp119I	TTCGAA	1 cut(s)	547
Bsp1286I	GDGCHC	4 cut(s)	204, 469, 571, 791
Bsp13I	TCCGGA	1 cut(s)	1349
Bsp143I	GATC	8 cut(s)	328, 393, 680, 757, 979, 1346, 1425, 1603
Bsp1720I	GCTNAGC	1 cut(s)	375
BspACI	CCGC	8 cut(s)	145, 449, 623, 1201, 1953, 2040, 2053, 2055
BspCNI	CTCAG	8 cut(s)	822, 1111, 1132, 1141, 1483, 1558, 1598, 1659
BspDI	ATCGAT	1 cut(s)	1345
BspEI	TCCGGA	1 cut(s)	1349
BspFNI	CGCG	1 cut(s)	2055
BspLI	GGNNCC	5 cut(s)	39, 363, 570, 806, 2035
BspMAI	CTGCAG	3 cut(s)	979, 1498, 1600
BspMI	ACCTGC	3 cut(s)	604, 1208, 1487
BspPI	GGATC	5 cut(s)	323, 401, 688, 765, 1341
BspQI	GCTCTTC	2 cut(s)	104, 245
BspT104I	TTCGAA	1 cut(s)	547
BspT107I	GGYRCC	3 cut(s)	361, 568, 2033
BspTNI	GGTCTC	1 cut(s)	182
BsrFI	RCCGGY	3 cut(s)	473, 1367, 2036
BsrI	ACTGG	3 cut(s)	559, 1281, 1867
BssAI	RCCGGY	3 cut(s)	473, 1367, 2036
BssECI	CCNNGG	4 cut(s)	276, 597, 1963, 2053
BssMI	GATC	8 cut(s)	328, 393, 680, 757, 979, 1346, 1425, 1603
BssNI	GRCGYC	1 cut(s)	362
BssSI	CACGAG	1 cut(s)	1269
BssT1I	CCWWGG	1 cut(s)	276
Bst2BI	CACGAG	1 cut(s)	1269
Bst2UI	CCWGG	2 cut(s)	598, 744
Bst4CI	ACNGT	2 cut(s)	577, 1611
Bst6I	CTCTTC	7 cut(s)	104, 245, 294, 707, 911, 1649, 1981
BstACI	GRCGYC	1 cut(s)	362
BstAPI	GCANNNNNTGC	1 cut(s)	1733
BstBAI	YACGTR	1 cut(s)	1322
BstBI	TTCGAA	1 cut(s)	547
BstC8I	GCNNGC	6 cut(s)	209, 469, 1009, 1369, 1636, 2038
BstDSI	CCRYGG	2 cut(s)	1963, 2053
BstENI	CCTNNNNNAGG	1 cut(s)	1750
BstF5I	GGATG	2 cut(s)	331, 840
BstFNI	CGCG	1 cut(s)	2055
BstH2I	RGCGCY	3 cut(s)	155, 365, 1799
BstHHI	GCGC	3 cut(s)	154, 364, 1798
BstKTI	GATC	8 cut(s)	331, 396, 683, 760, 982, 1349, 1428, 1606
BstMAI	GTCTC	3 cut(s)	182, 1115, 1534
BstMBI	GATC	8 cut(s)	328, 393, 680, 757, 979, 1346, 1425, 1603
BstNI	CCWGG	2 cut(s)	598, 744
BstNSI	RCATGY	1 cut(s)	1460
BstSCI	CCNGG	3 cut(s)	462, 596, 742
BstSFI	CTRYAG	4 cut(s)	9, 975, 1494, 1596
BstSLI	GKGCMC	1 cut(s)	571
BstUI	CGCG	1 cut(s)	2055
BstV1I	GCAGC	7 cut(s)	704, 1277, 1480, 1520, 1618, 1822, 1889
BstV2I	GAAGAC	2 cut(s)	183, 1338
BstX2I	RGATCY	3 cut(s)	680, 979, 1603
BstXI	CCANNNNNNTGG	2 cut(s)	604, 1261
BstYI	RGATCY	3 cut(s)	680, 979, 1603
Bsu15I	ATCGAT	1 cut(s)	1345
Bsu36I	CCTNAGG	1 cut(s)	1585
BsuI	GTATCC	1 cut(s)	551
BsuTUI	ATCGAT	1 cut(s)	1345
BtgI	CCRYGG	2 cut(s)	1963, 2053
BtsCI	GGATG	2 cut(s)	331, 840
BtsI	GCAGTG	2 cut(s)	1099, 1827
BtsIMutI	CAGTG	6 cut(s)	916, 1099, 1116, 1313, 1788, 1827
BveI	ACCTGC	3 cut(s)	604, 1208, 1487
Cac8I	GCNNGC	6 cut(s)	209, 469, 1009, 1369, 1636, 2038
CfoI	GCGC	3 cut(s)	154, 364, 1798
Cfr10I	RCCGGY	3 cut(s)	473, 1367, 2036
Cfr13I	GGNCC	2 cut(s)	460, 805
Cfr42I	CCGCGG	1 cut(s)	2056
ClaI	ATCGAT	1 cut(s)	1345
CseI	GACGC	2 cut(s)	1000, 1362
CspAI	ACCGGT	1 cut(s)	473
CviAII	CATG	4 cut(s)	421, 1160, 1457, 1685
DinI	GGCGCC	1 cut(s)	363
DpnI	GATC	8 cut(s)	330, 395, 682, 759, 981, 1348, 1427, 1605
DpnII	GATC	8 cut(s)	328, 393, 680, 757, 979, 1346, 1425, 1603
EaeI	YGGCCR	2 cut(s)	606, 1960
Eam1104I	CTCTTC	7 cut(s)	104, 245, 294, 707, 911, 1649, 1981
EarI	CTCTTC	7 cut(s)	104, 245, 294, 707, 911, 1649, 1981
EciI	GGCGGA	1 cut(s)	464
Ecl136II	GAGCTC	1 cut(s)	202
Eco130I	CCWWGG	1 cut(s)	276
Eco147I	AGGCCT	2 cut(s)	401, 1590
Eco24I	GRGCYC	2 cut(s)	204, 469
Eco31I	GGTCTC	1 cut(s)	182
Eco32I	GATATC	2 cut(s)	1075, 1282
Eco47I	GGWCC	1 cut(s)	805
Eco47III	AGCGCT	1 cut(s)	153
Eco53kI	GAGCTC	1 cut(s)	202
Eco57I	CTGAAG	4 cut(s)	158, 401, 892, 1860
Eco72I	CACGTG	1 cut(s)	1322
Eco81I	CCTNAGG	1 cut(s)	1585
EcoICRI	GAGCTC	1 cut(s)	202
EcoNI	CCTNNNNNAGG	1 cut(s)	1750
EcoO109I	RGGNCCY	1 cut(s)	805
EcoRII	CCWGG	2 cut(s)	596, 742
EcoRV	GATATC	2 cut(s)	1075, 1282
EcoT14I	CCWWGG	1 cut(s)	276
EcoT38I	GRGCYC	2 cut(s)	204, 469
EgeI	GGCGCC	1 cut(s)	363
EheI	GGCGCC	1 cut(s)	363
ErhI	CCWWGG	1 cut(s)	276
FaeI	CATG	4 cut(s)	424, 1163, 1460, 1688
FaqI	GGGAC	1 cut(s)	1196
FatI	CATG	4 cut(s)	420, 1159, 1456, 1684
FauI	CCCGC	1 cut(s)	1194
FblI	GTMKAC	1 cut(s)	1059
Fnu4HI	GCNGC	9 cut(s)	693, 1266, 1494, 1509, 1632, 1836, 1878, 2053, 2056
FokI	GGATG	2 cut(s)	338, 847
FriOI	GRGCYC	2 cut(s)	204, 469
Fsp4HI	GCNGC	9 cut(s)	693, 1266, 1494, 1509, 1632, 1836, 1878, 2053, 2056
FspBI	CTAG	4 cut(s)	866, 1044, 1053, 1404
GlaI	GCGC	3 cut(s)	153, 363, 1797
GluI	GCNGC	9 cut(s)	693, 1266, 1494, 1509, 1632, 1836, 1878, 2053, 2056
GsuI	CTGGAG	2 cut(s)	53, 1398
HaeII	RGCGCY	3 cut(s)	155, 365, 1799
HapII	CCGG	6 cut(s)	463, 474, 1350, 1368, 1763, 2037
HgaI	GACGC	2 cut(s)	1000, 1362
HhaI	GCGC	3 cut(s)	154, 364, 1798
Hin1I	GRCGYC	1 cut(s)	362
Hin1II	CATG	4 cut(s)	424, 1163, 1460, 1688
Hin6I	GCGC	3 cut(s)	152, 362, 1796
HinP1I	GCGC	3 cut(s)	152, 362, 1796
HincII	GTYRAC	1 cut(s)	522
HindII	GTYRAC	1 cut(s)	522
HpaI	GTTAAC	1 cut(s)	522
HpaII	CCGG	6 cut(s)	463, 474, 1350, 1368, 1763, 2037
HphI	GGTGA	3 cut(s)	328, 1456, 1681
Hpy166II	GTNNAC	6 cut(s)	408, 522, 1060, 1274, 1313, 1919
Hpy188III	TCNNGA	6 cut(s)	698, 1070, 1240, 1350, 1377, 1555
Hpy8I	GTNNAC	6 cut(s)	408, 522, 1060, 1274, 1313, 1919
Hpy99I	CGWCG	2 cut(s)	173, 176
HpyCH4III	ACNGT	2 cut(s)	577, 1611
HpyCH4IV	ACGT	6 cut(s)	15, 425, 852, 1062, 1321, 1482
HpySE526I	ACGT	6 cut(s)	15, 425, 852, 1062, 1321, 1482
Hsp92I	GRCGYC	1 cut(s)	362
Hsp92II	CATG	4 cut(s)	424, 1163, 1460, 1688
HspAI	GCGC	3 cut(s)	152, 362, 1796
KasI	GGCGCC	1 cut(s)	361
Kpn2I	TCCGGA	1 cut(s)	1349
KroI	GCCGGC	2 cut(s)	1367, 2036
KroNI	GCCGGC	2 cut(s)	1369, 2038
KspAI	GTTAAC	1 cut(s)	522
KspI	CCGCGG	1 cut(s)	2056
Kzo9I	GATC	8 cut(s)	328, 393, 680, 757, 979, 1346, 1425, 1603
LguI	GCTCTTC	2 cut(s)	104, 245
LmnI	GCTCC	6 cut(s)	43, 183, 200, 1163, 1471, 1570
Lsp1109I	GCAGC	7 cut(s)	704, 1277, 1480, 1520, 1618, 1822, 1889
LweI	GCATC	2 cut(s)	668, 1736
MaeI	CTAG	4 cut(s)	866, 1044, 1053, 1404
MaeII	ACGT	6 cut(s)	15, 425, 852, 1062, 1321, 1482
MaeIII	GTNAC	5 cut(s)	340, 883, 1207, 1659, 1687
MalI	GATC	8 cut(s)	330, 395, 682, 759, 981, 1348, 1427, 1605
MboI	GATC	8 cut(s)	328, 393, 680, 757, 979, 1346, 1425, 1603
MfeI	CAATTG	1 cut(s)	1476
MflI	RGATCY	3 cut(s)	680, 979, 1603
MhlI	GDGCHC	4 cut(s)	204, 469, 571, 791
MlsI	TGGCCA	1 cut(s)	608
MluNI	TGGCCA	1 cut(s)	608
Mly113I	GGCGCC	1 cut(s)	362
MlyI	GAGTC	6 cut(s)	83, 86, 137, 524, 1787, 1898
Mox20I	TGGCCA	1 cut(s)	608
MroI	TCCGGA	1 cut(s)	1349
MroNI	GCCGGC	2 cut(s)	1367, 2036
MroXI	GAANNNNTTC	1 cut(s)	861
MscI	TGGCCA	1 cut(s)	608
MseI	TTAA	3 cut(s)	521, 957, 1298
MslI	CAYNNNNRTG	1 cut(s)	1259
Msp20I	TGGCCA	1 cut(s)	608
MspA1I	CMGCKG	3 cut(s)	1201, 1880, 2055
MspI	CCGG	6 cut(s)	463, 474, 1350, 1368, 1763, 2037
MspR9I	CCNGG	3 cut(s)	464, 598, 744
MunI	CAATTG	1 cut(s)	1476
Mva1269I	GAATGC	1 cut(s)	1550
MvaI	CCWGG	2 cut(s)	598, 744
MvnI	CGCG	1 cut(s)	2055
NaeI	GCCGGC	2 cut(s)	1369, 2038
NarI	GGCGCC	1 cut(s)	362
NciI	CCSGG	1 cut(s)	464
NdeII	GATC	8 cut(s)	328, 393, 680, 757, 979, 1346, 1425, 1603
NgoMIV	GCCGGC	2 cut(s)	1367, 2036
NlaIII	CATG	4 cut(s)	424, 1163, 1460, 1688
NlaIV	GGNNCC	5 cut(s)	39, 363, 570, 806, 2035
NmeAIII	GCCGAG	2 cut(s)	183, 1938
NmuCI	GTSAC	3 cut(s)	1207, 1659, 1687
NspI	RCATGY	1 cut(s)	1460
NspV	TTCGAA	1 cut(s)	547
OliI	CACNNNNGTG	1 cut(s)	1259
PaqCI	CACCTGC	1 cut(s)	604
PceI	AGGCCT	2 cut(s)	401, 1590
PciSI	GCTCTTC	2 cut(s)	104, 245
PctI	GAATGC	1 cut(s)	1550
PdiI	GCCGGC	2 cut(s)	1369, 2038
PdmI	GAANNNNTTC	1 cut(s)	861
PfeI	GAWTC	4 cut(s)	236, 412, 1436, 1681
PinAI	ACCGGT	1 cut(s)	473
PkrI	GCNGC	9 cut(s)	694, 1267, 1495, 1510, 1633, 1837, 1879, 2054, 2057
PleI	GAGTC	6 cut(s)	83, 85, 136, 523, 1786, 1898
PluTI	GGCGCC	1 cut(s)	365
PmaCI	CACGTG	1 cut(s)	1322
PmlI	CACGTG	1 cut(s)	1322
PpsI	GAGTC	6 cut(s)	83, 85, 136, 523, 1786, 1898
Ppu21I	YACGTR	1 cut(s)	1322
PpuMI	RGGWCCY	1 cut(s)	805
PshBI	ATTAAT	1 cut(s)	1298
Psp124BI	GAGCTC	1 cut(s)	204
Psp5II	RGGWCCY	1 cut(s)	805
Psp6I	CCWGG	2 cut(s)	596, 742
PspCI	CACGTG	1 cut(s)	1322
PspGI	CCWGG	2 cut(s)	596, 742
PspN4I	GGNNCC	5 cut(s)	39, 363, 570, 806, 2035
PspPI	GGNCC	2 cut(s)	460, 805
PspPPI	RGGWCCY	1 cut(s)	805
PsrI	GAACNNNNNNTAC	2 cut(s)	876, 908
PstI	CTGCAG	3 cut(s)	979, 1498, 1600
PsuI	RGATCY	3 cut(s)	680, 979, 1603
PvuII	CAGCTG	1 cut(s)	1880
RseI	CAYNNNNRTG	1 cut(s)	1259
SacI	GAGCTC	1 cut(s)	204
SacII	CCGCGG	1 cut(s)	2056
SapI	GCTCTTC	2 cut(s)	104, 245
SaqAI	TTAA	3 cut(s)	521, 957, 1298
SatI	GCNGC	9 cut(s)	693, 1266, 1494, 1509, 1632, 1836, 1878, 2053, 2056
Sau3AI	GATC	8 cut(s)	328, 393, 680, 757, 979, 1346, 1425, 1603
Sau96I	GGNCC	2 cut(s)	460, 805
SchI	GAGTC	6 cut(s)	83, 86, 137, 524, 1787, 1898
ScrFI	CCNGG	3 cut(s)	464, 598, 744
SduI	GDGCHC	4 cut(s)	204, 469, 571, 791
SfaNI	GCATC	2 cut(s)	668, 1736
SfcI	CTRYAG	4 cut(s)	9, 975, 1494, 1596
SfoI	GGCGCC	1 cut(s)	363
Sfr303I	CCGCGG	1 cut(s)	2056
SfuI	TTCGAA	1 cut(s)	547
SgrBI	CCGCGG	1 cut(s)	2056
SinI	GGWCC	1 cut(s)	805
SmiMI	CAYNNNNRTG	1 cut(s)	1259
SmlI	CTYRAG	3 cut(s)	696, 784, 1747
SmoI	CTYRAG	3 cut(s)	696, 784, 1747
SseBI	AGGCCT	2 cut(s)	401, 1590
SsiI	CCGC	8 cut(s)	145, 449, 623, 1201, 1953, 2040, 2053, 2055
SspDI	GGCGCC	1 cut(s)	361
SspI	AATATT	1 cut(s)	940
SspMI	CTAG	4 cut(s)	866, 1044, 1053, 1404
SstI	GAGCTC	1 cut(s)	204
StuI	AGGCCT	2 cut(s)	401, 1590
StyD4I	CCNGG	3 cut(s)	462, 596, 742
StyI	CCWWGG	1 cut(s)	276
TaaI	ACNGT	2 cut(s)	577, 1611
TaiI	ACGT	6 cut(s)	18, 428, 855, 1065, 1324, 1485
TaqI	TCGA	9 cut(s)	68, 266, 396, 429, 547, 1241, 1345, 1428, 1776
TauI	GCSGC	2 cut(s)	2055, 2058
TfiI	GAWTC	4 cut(s)	236, 412, 1436, 1681
Tru1I	TTAA	3 cut(s)	521, 957, 1298
Tru9I	TTAA	3 cut(s)	521, 957, 1298
TscAI	CASTG	6 cut(s)	916, 1099, 1123, 1313, 1788, 1827
TseFI	GTSAC	3 cut(s)	1207, 1659, 1687
TseI	GCWGC	7 cut(s)	692, 1265, 1493, 1508, 1631, 1835, 1877
Tsp45I	GTSAC	3 cut(s)	1207, 1659, 1687
TspDTI	ATGAA	5 cut(s)	74, 828, 837, 1008, 1016
TspRI	CASTG	6 cut(s)	916, 1099, 1123, 1313, 1788, 1827
VpaK11BI	GGWCC	1 cut(s)	805
VspI	ATTAAT	1 cut(s)	1298
XagI	CCTNNNNNAGG	1 cut(s)	1750
XapI	RAATTY	5 cut(s)	542, 632, 861, 1235, 1448
XceI	RCATGY	1 cut(s)	1460
XcmI	CCANNNNNNNNNTGG	1 cut(s)	601
XmiI	GTMKAC	1 cut(s)	1059
XmnI	GAANNNNTTC	1 cut(s)	861
XspI	CTAG	4 cut(s)	866, 1044, 1053, 1404

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.