DEAD-box ATP-dependent RNA helicase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 257 |
| Acc65I | GGTACC | 1 cut(s) | 603 |
| AccB1I | GGYRCC | 2 cut(s) | 103, 603 |
| AciI | CCGC | 2 cut(s) | 264, 422 |
| AclWI | GGATC | 2 cut(s) | 255, 338 |
| AcoI | YGGCCR | 1 cut(s) | 489 |
| AcyI | GRCGYC | 1 cut(s) | 104 |
| AdeI | CACNNNGTG | 1 cut(s) | 342 |
| AfaI | GTAC | 2 cut(s) | 224, 605 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 326, 327 |
| AgsI | TTSAA | 3 cut(s) | 16, 376, 620 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 529, 561 |
| AleI | CACNNNNGTG | 1 cut(s) | 338 |
| AluBI | AGCT | 6 cut(s) | 288, 380, 464, 544, 572, 581 |
| AluI | AGCT | 6 cut(s) | 288, 380, 464, 544, 572, 581 |
| Alw21I | GWGCWC | 2 cut(s) | 347, 400 |
| Alw26I | GTCTC | 1 cut(s) | 298 |
| AlwI | GGATC | 2 cut(s) | 255, 338 |
| AoxI | GGCC | 4 cut(s) | 168, 299, 317, 489 |
| ApaI | GGGCCC | 1 cut(s) | 321 |
| Asp718I | GGTACC | 1 cut(s) | 603 |
| AspLEI | GCGC | 2 cut(s) | 106, 148 |
| AspS9I | GGNCC | 4 cut(s) | 37, 299, 317, 318 |
| AsuHPI | GGTGA | 3 cut(s) | 64, 325, 646 |
| AvaII | GGWCC | 1 cut(s) | 37 |
| AxyI | CCTNAGG | 1 cut(s) | 41 |
| BaeGI | GKGCMC | 1 cut(s) | 321 |
| BanI | GGYRCC | 2 cut(s) | 103, 603 |
| BanII | GRGCYC | 1 cut(s) | 321 |
| BauI | CACGAG | 1 cut(s) | 359 |
| BbsI | GAAGAC | 1 cut(s) | 59 |
| Bbv12I | GWGCWC | 2 cut(s) | 347, 400 |
| BceAI | ACGGC | 2 cut(s) | 251, 476 |
| BclI | TGATCA | 1 cut(s) | 69 |
| BcoDI | GTCTC | 1 cut(s) | 298 |
| BfoI | RGCGCY | 2 cut(s) | 107, 149 |
| Bme18I | GGWCC | 1 cut(s) | 37 |
| BmgT120I | GGNCC | 4 cut(s) | 37, 299, 317, 318 |
| BmiI | GGNNCC | 3 cut(s) | 105, 319, 605 |
| BpiI | GAAGAC | 1 cut(s) | 59 |
| BsaHI | GRCGYC | 1 cut(s) | 104 |
| BsaJI | CCNNGG | 3 cut(s) | 141, 165, 599 |
| BsaWI | WCCGGW | 1 cut(s) | 400 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 11, 41 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 326, 327 |
| Bse21I | CCTNAGG | 1 cut(s) | 41 |
| BseDI | CCNNGG | 3 cut(s) | 141, 165, 599 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 326, 327 |
| BseMII | CTCAG | 1 cut(s) | 32 |
| BseRI | GAGGAG | 3 cut(s) | 35, 440, 450 |
| BseSI | GKGCMC | 1 cut(s) | 321 |
| BshFI | GGCC | 4 cut(s) | 170, 301, 319, 491 |
| BshNI | GGYRCC | 2 cut(s) | 103, 603 |
| BsiHKAI | GWGCWC | 2 cut(s) | 347, 400 |
| BsiSI | CCGG | 1 cut(s) | 401 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 326, 327 |
| BsmAI | GTCTC | 1 cut(s) | 298 |
| BsnI | GGCC | 4 cut(s) | 170, 301, 319, 491 |
| Bsp120I | GGGCCC | 1 cut(s) | 317 |
| Bsp1286I | GDGCHC | 3 cut(s) | 321, 347, 400 |
| Bsp143I | GATC | 3 cut(s) | 69, 260, 330 |
| BspACI | CCGC | 2 cut(s) | 264, 422 |
| BspANI | GGCC | 4 cut(s) | 170, 301, 319, 491 |
| BspCNI | CTCAG | 1 cut(s) | 33 |
| BspLI | GGNNCC | 3 cut(s) | 105, 319, 605 |
| BspPI | GGATC | 2 cut(s) | 255, 338 |
| BspT107I | GGYRCC | 2 cut(s) | 103, 603 |
| BssECI | CCNNGG | 3 cut(s) | 141, 165, 599 |
| BssMI | GATC | 3 cut(s) | 69, 260, 330 |
| BssNI | GRCGYC | 1 cut(s) | 104 |
| BssSI | CACGAG | 1 cut(s) | 359 |
| Bst2BI | CACGAG | 1 cut(s) | 359 |
| Bst4CI | ACNGT | 2 cut(s) | 58, 340 |
| Bst6I | CTCTTC | 1 cut(s) | 199 |
| BstACI | GRCGYC | 1 cut(s) | 104 |
| BstDEI | CTNAG | 1 cut(s) | 41 |
| BstH2I | RGCGCY | 2 cut(s) | 107, 149 |
| BstHHI | GCGC | 2 cut(s) | 106, 148 |
| BstKTI | GATC | 3 cut(s) | 72, 263, 333 |
| BstMAI | GTCTC | 1 cut(s) | 298 |
| BstMBI | GATC | 3 cut(s) | 69, 260, 330 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 578 |
| BstSLI | GKGCMC | 1 cut(s) | 321 |
| BstV2I | GAAGAC | 1 cut(s) | 59 |
| BstX2I | RGATCY | 1 cut(s) | 260 |
| BstYI | RGATCY | 1 cut(s) | 260 |
| Bsu36I | CCTNAGG | 1 cut(s) | 41 |
| BsuRI | GGCC | 4 cut(s) | 170, 301, 319, 491 |
| BtgZI | GCGATG | 1 cut(s) | 106 |
| CfoI | GCGC | 2 cut(s) | 106, 148 |
| Cfr13I | GGNCC | 4 cut(s) | 37, 299, 317, 318 |
| Csp6I | GTAC | 2 cut(s) | 223, 604 |
| CviAII | CATG | 1 cut(s) | 76 |
| CviQI | GTAC | 2 cut(s) | 223, 604 |
| DdeI | CTNAG | 1 cut(s) | 41 |
| DinI | GGCGCC | 1 cut(s) | 105 |
| DpnI | GATC | 3 cut(s) | 71, 262, 332 |
| DpnII | GATC | 3 cut(s) | 69, 260, 330 |
| DraIII | CACNNNGTG | 1 cut(s) | 342 |
| EaeI | YGGCCR | 1 cut(s) | 489 |
| Eam1104I | CTCTTC | 1 cut(s) | 199 |
| EarI | CTCTTC | 1 cut(s) | 199 |
| EciI | GGCGGA | 1 cut(s) | 253 |
| Eco24I | GRGCYC | 1 cut(s) | 321 |
| Eco47I | GGWCC | 1 cut(s) | 37 |
| Eco81I | CCTNAGG | 1 cut(s) | 41 |
| EcoO109I | RGGNCCY | 2 cut(s) | 37, 318 |
| EcoT38I | GRGCYC | 1 cut(s) | 321 |
| EgeI | GGCGCC | 1 cut(s) | 105 |
| EheI | GGCGCC | 1 cut(s) | 105 |
| FaeI | CATG | 1 cut(s) | 79 |
| FaiI | YATR | 7 cut(s) | 77, 257, 272, 285, 501, 516, 649 |
| FatI | CATG | 1 cut(s) | 75 |
| FbaI | TGATCA | 1 cut(s) | 69 |
| FriOI | GRGCYC | 1 cut(s) | 321 |
| GlaI | GCGC | 2 cut(s) | 105, 147 |
| HaeII | RGCGCY | 2 cut(s) | 107, 149 |
| HaeIII | GGCC | 4 cut(s) | 170, 301, 319, 491 |
| HapII | CCGG | 1 cut(s) | 401 |
| HhaI | GCGC | 2 cut(s) | 106, 148 |
| Hin1I | GRCGYC | 1 cut(s) | 104 |
| Hin1II | CATG | 1 cut(s) | 79 |
| Hin6I | GCGC | 2 cut(s) | 104, 146 |
| HinP1I | GCGC | 2 cut(s) | 104, 146 |
| HindIII | AAGCTT | 2 cut(s) | 378, 542 |
| HinfI | GANTC | 1 cut(s) | 109 |
| HpaII | CCGG | 1 cut(s) | 401 |
| HphI | GGTGA | 3 cut(s) | 64, 325, 646 |
| Hpy166II | GTNNAC | 1 cut(s) | 553 |
| Hpy188I | TCNGA | 2 cut(s) | 457, 600 |
| Hpy188III | TCNNGA | 4 cut(s) | 40, 67, 182, 620 |
| Hpy8I | GTNNAC | 1 cut(s) | 553 |
| Hpy99I | CGWCG | 2 cut(s) | 299, 497 |
| HpyAV | CCTTC | 3 cut(s) | 10, 28, 531 |
| HpyCH4III | ACNGT | 2 cut(s) | 58, 340 |
| HpyCH4V | TGCA | 1 cut(s) | 528 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 578 |
| HpyF3I | CTNAG | 1 cut(s) | 41 |
| Hsp92I | GRCGYC | 1 cut(s) | 104 |
| Hsp92II | CATG | 1 cut(s) | 79 |
| HspAI | GCGC | 2 cut(s) | 104, 146 |
| KasI | GGCGCC | 1 cut(s) | 103 |
| KpnI | GGTACC | 1 cut(s) | 607 |
| Ksp22I | TGATCA | 1 cut(s) | 69 |
| Kzo9I | GATC | 3 cut(s) | 69, 260, 330 |
| LmnI | GCTCC | 2 cut(s) | 403, 427 |
| LpnPI | CCDG | 3 cut(s) | 53, 414, 620 |
| MalI | GATC | 3 cut(s) | 71, 262, 332 |
| MboI | GATC | 3 cut(s) | 69, 260, 330 |
| MboII | GAAGA | 7 cut(s) | 43, 55, 64, 104, 153, 186, 622 |
| MflI | RGATCY | 1 cut(s) | 260 |
| MhlI | GDGCHC | 3 cut(s) | 321, 347, 400 |
| MluCI | AATT | 1 cut(s) | 558 |
| Mly113I | GGCGCC | 1 cut(s) | 104 |
| MslI | CAYNNNNRTG | 1 cut(s) | 338 |
| MspI | CCGG | 1 cut(s) | 401 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 578 |
| NarI | GGCGCC | 1 cut(s) | 104 |
| NdeII | GATC | 3 cut(s) | 69, 260, 330 |
| NlaIII | CATG | 1 cut(s) | 79 |
| NlaIV | GGNNCC | 3 cut(s) | 105, 319, 605 |
| NmeAIII | GCCGAG | 5 cut(s) | 80, 122, 134, 146, 327 |
| OliI | CACNNNNGTG | 1 cut(s) | 338 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 123 |
| PfeI | GAWTC | 1 cut(s) | 109 |
| PluTI | GGCGCC | 1 cut(s) | 107 |
| PpuMI | RGGWCCY | 1 cut(s) | 37 |
| PsiI | TTATAA | 1 cut(s) | 257 |
| Psp5II | RGGWCCY | 1 cut(s) | 37 |
| PspN4I | GGNNCC | 3 cut(s) | 105, 319, 605 |
| PspOMI | GGGCCC | 1 cut(s) | 317 |
| PspPI | GGNCC | 4 cut(s) | 37, 299, 317, 318 |
| PspPPI | RGGWCCY | 1 cut(s) | 37 |
| PsuI | RGATCY | 1 cut(s) | 260 |
| RsaI | GTAC | 2 cut(s) | 224, 605 |
| RsaNI | GTAC | 2 cut(s) | 223, 604 |
| RseI | CAYNNNNRTG | 1 cut(s) | 338 |
| Sau3AI | GATC | 3 cut(s) | 69, 260, 330 |
| Sau96I | GGNCC | 4 cut(s) | 37, 299, 317, 318 |
| SduI | GDGCHC | 3 cut(s) | 321, 347, 400 |
| SetI | ASST | 9 cut(s) | 39, 290, 382, 466, 546, 574, 583, 605, 636 |
| SfoI | GGCGCC | 1 cut(s) | 105 |
| SinI | GGWCC | 1 cut(s) | 37 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 338 |
| Sse9I | AATT | 1 cut(s) | 558 |
| SsiI | CCGC | 2 cut(s) | 264, 422 |
| SspDI | GGCGCC | 1 cut(s) | 103 |
| TaaI | ACNGT | 2 cut(s) | 58, 340 |
| TaqI | TCGA | 3 cut(s) | 29, 183, 567 |
| TasI | AATT | 1 cut(s) | 558 |
| TfiI | GAWTC | 1 cut(s) | 109 |
| TspDTI | ATGAA | 1 cut(s) | 460 |
| VpaK11BI | GGWCC | 1 cut(s) | 37 |