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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 216 |
| AccII | CGCG | 1 cut(s) | 62 |
| AcsI | RAATTY | 1 cut(s) | 181 |
| AfaI | GTAC | 1 cut(s) | 70 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 216 |
| AgsI | TTSAA | 3 cut(s) | 32, 92, 117 |
| AluBI | AGCT | 2 cut(s) | 107, 254 |
| AluI | AGCT | 2 cut(s) | 107, 254 |
| AoxI | GGCC | 1 cut(s) | 128 |
| ApeKI | GCWGC | 2 cut(s) | 4, 251 |
| ApoI | RAATTY | 1 cut(s) | 181 |
| AseI | ATTAAT | 1 cut(s) | 197 |
| BfaI | CTAG | 2 cut(s) | 126, 255 |
| BisI | GCNGC | 2 cut(s) | 5, 252 |
| BlsI | GCNGC | 2 cut(s) | 6, 253 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 216 |
| BseGI | GGATG | 2 cut(s) | 18, 225 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 216 |
| Bsh1236I | CGCG | 1 cut(s) | 62 |
| BshFI | GGCC | 1 cut(s) | 130 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 216 |
| BsmI | GAATGC | 1 cut(s) | 45 |
| BsnI | GGCC | 1 cut(s) | 130 |
| BspANI | GGCC | 1 cut(s) | 130 |
| BspFNI | CGCG | 1 cut(s) | 62 |
| BspHI | TCATGA | 1 cut(s) | 177 |
| BstC8I | GCNNGC | 1 cut(s) | 132 |
| BstF5I | GGATG | 2 cut(s) | 18, 225 |
| BstFNI | CGCG | 1 cut(s) | 62 |
| BstUI | CGCG | 1 cut(s) | 62 |
| BsuRI | GGCC | 1 cut(s) | 130 |
| BtsCI | GGATG | 2 cut(s) | 18, 225 |
| Cac8I | GCNNGC | 1 cut(s) | 132 |
| CciI | TCATGA | 1 cut(s) | 177 |
| CseI | GACGC | 1 cut(s) | 51 |
| Csp6I | GTAC | 1 cut(s) | 69 |
| CviAII | CATG | 2 cut(s) | 8, 178 |
| CviJI | RGCY | 4 cut(s) | 107, 130, 134, 254 |
| CviKI_1 | RGCY | 4 cut(s) | 107, 130, 134, 254 |
| CviQI | GTAC | 1 cut(s) | 69 |
| FaeI | CATG | 2 cut(s) | 11, 181 |
| FaiI | YATR | 7 cut(s) | 9, 22, 51, 56, 169, 179, 235 |
| FatI | CATG | 2 cut(s) | 7, 177 |
| Fnu4HI | GCNGC | 2 cut(s) | 5, 252 |
| FokI | GGATG | 2 cut(s) | 25, 232 |
| Fsp4HI | GCNGC | 2 cut(s) | 5, 252 |
| FspBI | CTAG | 2 cut(s) | 126, 255 |
| GluI | GCNGC | 2 cut(s) | 5, 252 |
| HaeIII | GGCC | 1 cut(s) | 130 |
| HgaI | GACGC | 1 cut(s) | 51 |
| Hin1II | CATG | 2 cut(s) | 11, 181 |
| HinfI | GANTC | 1 cut(s) | 174 |
| Hpy188III | TCNNGA | 1 cut(s) | 178 |
| Hpy99I | CGWCG | 1 cut(s) | 67 |
| HpyCH4V | TGCA | 2 cut(s) | 7, 20 |
| Hsp92II | CATG | 2 cut(s) | 11, 181 |
| LpnPI | CCDG | 2 cut(s) | 144, 148 |
| MaeI | CTAG | 2 cut(s) | 126, 255 |
| MluCI | AATT | 7 cut(s) | 26, 87, 99, 164, 181, 194, 211 |
| MseI | TTAA | 1 cut(s) | 197 |
| Mva1269I | GAATGC | 1 cut(s) | 45 |
| MvnI | CGCG | 1 cut(s) | 62 |
| NlaIII | CATG | 2 cut(s) | 11, 181 |
| PagI | TCATGA | 1 cut(s) | 177 |
| PctI | GAATGC | 1 cut(s) | 45 |
| PfeI | GAWTC | 1 cut(s) | 174 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 216 |
| PkrI | GCNGC | 2 cut(s) | 6, 253 |
| PshBI | ATTAAT | 1 cut(s) | 197 |
| RsaI | GTAC | 1 cut(s) | 70 |
| RsaNI | GTAC | 1 cut(s) | 69 |
| SaqAI | TTAA | 1 cut(s) | 197 |
| SatI | GCNGC | 2 cut(s) | 5, 252 |
| SetI | ASST | 3 cut(s) | 109, 145, 256 |
| Sse9I | AATT | 7 cut(s) | 26, 87, 99, 164, 181, 194, 211 |
| SspMI | CTAG | 2 cut(s) | 126, 255 |
| TasI | AATT | 7 cut(s) | 26, 87, 99, 164, 181, 194, 211 |
| TfiI | GAWTC | 1 cut(s) | 174 |
| Tru1I | TTAA | 1 cut(s) | 197 |
| Tru9I | TTAA | 1 cut(s) | 197 |
| TseI | GCWGC | 2 cut(s) | 4, 251 |
| TspDTI | ATGAA | 1 cut(s) | 194 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 216 |
| VspI | ATTAAT | 1 cut(s) | 197 |
| XapI | RAATTY | 1 cut(s) | 181 |
| XspI | CTAG | 2 cut(s) | 126, 255 |