Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0000604

Physical Location & Seq

Genomic Coordinates Forward (+)

28623282 .. 28626410

Gene Structure 3129 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1187900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1344 bp

ATGGGATCGTACAGGAGAGCAAAGCTCGCACTCGACGCCTTCCGCGGCTTCACGGCGAGGATTGCCCCCAAAGCCGCCGCCAGAGAGCCCATCTCTCGCGTTTTCTCAACTGGGTCTTCCGCTTCCGTCTCCAACTCAGCTAAGTTTTCTGGGTTTTCTTCCAATTCTTCAATTTTACACAAATCAATTCCGCAGCTTGGTAGAATCACAAGAACAACACAGTACAGAAGCCCCTTTCTTGATTGCACTAGGAGATATTACTATGTCGACCGGAAGCGGGTTCACCACTTCAAGCCGAGAGGCCCGCGGAGGTGGTTTGAGAGTCCCAGGAACGTTTTGATTGTGGGTCTGGTGGGTTCTGGGGTTTTTATCACCGTGTATTTTGGGAATTTGGAGACGATTCCGTACACGAAGCGAACCCATTTTATAATTTTGTCGAAAGCCTTGGAGAAGAGGTTGGGGGAGAGCCAATTCCAGCAAATGAAAGAGAGTTTCAAGGGGGACATTTTGCCCGCCATACATCCGGATAGCGTGCGGGTTCGGTTGATTGCCAAGGATATAATAGAGGCTTTGCAGAGAGGGTTGAGCCATGACGTGACCTGGACTGACGTGGACTATGCGTCCGGTAAAGTGGAGCTGGCACATGAGAGTGGTGGCAAGGACACTTTGATGGCACTGCAGGATCCTCCGGAGGAGGGTAAGTGGTCCCGTGAGGATGAGATTCTTGATGATCGATGGATTGAGAAGAGTAGGAAGACAAGTAGGGAGAGAGGTTTGAAAGCTGCCACTTCGCATTTGGATCATTTGAATTGGGAGGTTTTGGTGGTGGATAAGCCCATTGTCAATGCATTTTGCTTGCCGGGTGGGAAGATTGTCGTCTTCACGGGGTTGCTCAAGCATTTTACAAGTGATGCCGAGATAGCGACGATCATTGGTCATGAGGTTGGCCATGCTGTGGCTCGACATTCTGCAGAGACTATTACAAAGAACCTGTGGTTTGCAATTCTGCAACTGGTGCTTTATCAATTTGTTACGCCTGATGTTGTCAACACAATGTCCAATCTTTTCTTGAGGCTTCCTTTCTCCCGAAGGATGGAAATTGAAGCGGATCACATTGGGCTTCTGTTGGTTGCTTCTGCTGGATATGATCCCAGAGTGGCTCCAACTGTGTACGAGAAGTTGAGCAAGGTTACTGGTGGTGAATCTGCACTCAGAGATTATCTTTCAACCCATCCATCCGGGAAAAAGAGAGCTGAATTGCTGGCTCAAGCTAAGATCATGGAAGAAGCACTCGCTATATACAGGGATGCAGGAGCAGGACGTGGGGTTGAAGGCTTTCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0006508 GO:0006515 GO:0006807 GO:0006996 GO:0007005 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009056 GO:0009057 GO:0009987 GO:0016020 GO:0016043 GO:0016787 GO:0019538 GO:0019866 GO:0023052 GO:0030163 GO:0031090 GO:0031929 GO:0031966 GO:0031967 GO:0031975 GO:0035556 GO:0035694 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051603 GO:0051716 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M48

Protein Analysis

448

Amino Acids

50.04

Weight (kDa)

9.59

Isoelectric Point (pI)

39.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M48	PF01435	261 - 422	1.7e-31	Peptidase family M48

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000604)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51740 AT5G51740 AT5G51740
fragaria_vesca	FvH4_7g28431 FvH4_7g28432
malus_domestica	MD01G1187900.v1.1 MD07G1015000.v1.1 MD07G1260500.v1.1
prunus_persica	Prupe.2G284300_v2.0.a1 Prupe.2G284400_v2.0.a1
pyrus_communis	pycom01g19990 pycom07g23380 pycom07g23540 pycom07g23740
rosa_chinensis	RchiOBHm_Chr1g0342951 RchiOBHm_Chr1g0375391 RchiOBHm_Chr1g0375401 RchiOBHm_Chr1g0375411 RchiOBHm_Chr2g0123811 RchiOBHm_Chr4g0391111 RchiOBHm_Chr4g0406741 RchiOBHm_Chr5g0031101 RchiOBHm_Chr7g0214411
rosa_laevigata	RLG00000004191 RLG00000019338 RLG00000026663 RLG00000026664
rosa_multiflora	Rmu_sc0000554.1_g000043 Rmu_sc0000554.1_g000047 Rmu_sc0001966.1_g000052 Rmu_sc0002169.1_g000012 Rmu_sc0006244.1_g000048 Rmu_sc0007176.1_g000002 Rmu_sc0014983.1_g000004 Rmu_sc0020175.1_g000003 Rmu_sc0021990.1_g000003 Rmu_ssc0000106.1_g000001
rosa_roxburghii	Rroxscaffold_1G00029630 Rroxscaffold_1G00049160 Rroxscaffold_3G00235470 Rroxscaffold_3G00235480 Rroxscaffold_4G00282790 Rroxscaffold_4G00282800 Rroxscaffold_5G00357450 Rroxscaffold_5G00360550
rosa_rugosa	Rorug01G0390800 Rorug01G0390800 Rorug06G0413000
rosa_samantha	Rh1AG177000 Rh1AG401300 Rh1AG401400 Rh1BG362700 Rh1BG363000 Rh1BG363100 Rh1BG366200 Rh1CG376300 Rh1CG378900 Rh1DG069900 Rh1DG393500 Rh1DG393800 Rh1DG393900 Rh1DG396800 Rh2AG166900 Rh2DG172200 Rh2DG463600 Rh3CG274900 Rh4BG226700 Rh5AG215800 Rh5AG215900 Rh5BG215300 Rh5CG238800 Rh5DG219700 Rh5DG219800 Rh6BG136300 Rh6CG134700 Rh6DG121000 Rh7CG183600 Rh7DG190700
rosa_wichuraiana	Rw1G035620 Rw1G035630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	428
AccB7I	CCANNNNNTGG	1 cut(s)	955
AccI	GTMKAC	1 cut(s)	267
AccII	CGCG	3 cut(s)	45, 99, 307
AccIII	TCCGGA	2 cut(s)	523, 688
AclI	AACGTT	1 cut(s)	333
AclWI	GGATC	6 cut(s)	13, 677, 690, 807, 1116, 1142
AcoI	YGGCCR	1 cut(s)	946
AcsI	RAATTY	1 cut(s)	388
AcyI	GRCGYC	1 cut(s)	36
AfaI	GTAC	4 cut(s)	11, 224, 407, 1172
AfiI	CCNNNNNNNGG	5 cut(s)	197, 277, 695, 955, 1093
AgsI	TTSAA	8 cut(s)	171, 292, 496, 778, 808, 1103, 1227, 1331
AhdI	GACNNNNNGTC	1 cut(s)	619
AjiI	CACGTC	3 cut(s)	595, 610, 1322
AjnI	CCWGG	2 cut(s)	326, 599
AluBI	AGCT	7 cut(s)	25, 140, 196, 637, 782, 1253, 1271
AluI	AGCT	7 cut(s)	25, 140, 196, 637, 782, 1253, 1271
Alw26I	GTCTC	3 cut(s)	133, 389, 968
AlwI	GGATC	6 cut(s)	13, 677, 690, 807, 1116, 1142
Aor13HI	TCCGGA	2 cut(s)	523, 688
AoxI	GGCC	2 cut(s)	301, 946
ApeKI	GCWGC	2 cut(s)	193, 782
ApoI	RAATTY	1 cut(s)	388
Asp700I	GAANNNNTTC	1 cut(s)	1335
AspS9I	GGNCC	2 cut(s)	302, 705
AsuC2I	CCSGG	2 cut(s)	861, 1240
AsuHPI	GGTGA	3 cut(s)	275, 364, 1211
AvaII	GGWCC	1 cut(s)	705
BalI	TGGCCA	1 cut(s)	948
BamHI	GGATCC	1 cut(s)	682
BanII	GRGCYC	1 cut(s)	90
BbsI	GAAGAC	3 cut(s)	108, 761, 871
BbvI	GCAGC	2 cut(s)	205, 769
BccI	CCATC	6 cut(s)	98, 664, 729, 1087, 1239, 1243
BceAI	ACGGC	1 cut(s)	69
BciT130I	CCWGG	2 cut(s)	328, 601
BcnI	CCSGG	2 cut(s)	861, 1240
BcoDI	GTCTC	3 cut(s)	133, 389, 968
BfaI	CTAG	1 cut(s)	249
BfmI	CTRYAG	2 cut(s)	677, 969
BisI	GCNGC	5 cut(s)	46, 75, 78, 194, 783
BlsI	GCNGC	5 cut(s)	47, 76, 79, 195, 784
Bme1390I	CCNGG	4 cut(s)	328, 601, 861, 1240
Bme18I	GGWCC	1 cut(s)	705
BmeRI	GACNNNNNGTC	1 cut(s)	619
BmgBI	CACGTC	3 cut(s)	595, 610, 1322
BmgT120I	GGNCC	2 cut(s)	302, 705
BmiI	GGNNCC	3 cut(s)	684, 707, 1161
BmrFI	CCNGG	4 cut(s)	328, 601, 861, 1240
BmrI	ACTGGG	1 cut(s)	120
BmsI	GCATC	2 cut(s)	901, 1297
BmuI	ACTGGG	1 cut(s)	120
BpiI	GAAGAC	3 cut(s)	108, 761, 871
BplI	GAGNNNNNCTC	4 cut(s)	9, 41, 77, 109
BpuEI	CTTGAG	3 cut(s)	878, 1090, 1251
BpuMI	CCSGG	2 cut(s)	861, 1240
Bsa29I	ATCGAT	1 cut(s)	733
BsaHI	GRCGYC	1 cut(s)	36
BsaJI	CCNNGG	5 cut(s)	43, 305, 326, 444, 552
BsaWI	WCCGGW	4 cut(s)	270, 523, 623, 688
BsaXI	ACNNNNNCTCC	4 cut(s)	440, 470, 686, 716
Bsc4I	CCNNNNNNNGG	5 cut(s)	197, 277, 695, 955, 1093
Bse1I	ACTGG	3 cut(s)	115, 1017, 1198
BseAI	TCCGGA	2 cut(s)	523, 688
BseBI	CCWGG	2 cut(s)	328, 601
BseCI	ATCGAT	1 cut(s)	733
BseDI	CCNNGG	5 cut(s)	43, 305, 326, 444, 552
BseGI	GGATG	6 cut(s)	520, 721, 1098, 1231, 1235, 1312
BseLI	CCNNNNNNNGG	5 cut(s)	197, 277, 695, 955, 1093
BseMII	CTCAG	2 cut(s)	150, 1225
BseNI	ACTGG	3 cut(s)	115, 1017, 1198
BseRI	GAGGAG	1 cut(s)	707
BseXI	GCAGC	2 cut(s)	205, 769
BsgI	GTGCAG	1 cut(s)	1191
Bsh1236I	CGCG	3 cut(s)	45, 99, 307
Bsh1285I	CGRYCG	1 cut(s)	271
BshFI	GGCC	2 cut(s)	303, 948
BshVI	ATCGAT	1 cut(s)	733
BsiEI	CGRYCG	1 cut(s)	271
BsiSI	CCGG	6 cut(s)	271, 524, 624, 689, 860, 1239
BslFI	GGGAC	3 cut(s)	309, 515, 691
BslI	CCNNNNNNNGG	5 cut(s)	197, 277, 695, 955, 1093
BsmAI	GTCTC	3 cut(s)	133, 389, 968
BsmBI	CGTCTC	2 cut(s)	133, 389
BsmFI	GGGAC	3 cut(s)	309, 515, 691
BsnI	GGCC	2 cut(s)	303, 948
Bsp1286I	GDGCHC	1 cut(s)	90
Bsp13I	TCCGGA	2 cut(s)	523, 688
Bsp143I	GATC	8 cut(s)	5, 682, 730, 799, 927, 1108, 1147, 1275
BspANI	GGCC	2 cut(s)	303, 948
BspCNI	CTCAG	2 cut(s)	149, 1224
BspDI	ATCGAT	1 cut(s)	733
BspEI	TCCGGA	2 cut(s)	523, 688
BspFNI	CGCG	3 cut(s)	45, 99, 307
BspHI	TCATGA	1 cut(s)	937
BspLI	GGNNCC	3 cut(s)	684, 707, 1161
BspMAI	CTGCAG	2 cut(s)	681, 973
BspPI	GGATC	6 cut(s)	13, 677, 690, 807, 1116, 1142
BsrI	ACTGG	3 cut(s)	115, 1017, 1198
BssECI	CCNNGG	5 cut(s)	43, 305, 326, 444, 552
BssMI	GATC	8 cut(s)	5, 682, 730, 799, 927, 1108, 1147, 1275
BssNI	GRCGYC	1 cut(s)	36
BssT1I	CCWWGG	2 cut(s)	444, 552
Bst2UI	CCWGG	2 cut(s)	328, 601
Bst4CI	ACNGT	3 cut(s)	222, 376, 1168
Bst6I	CTCTTC	2 cut(s)	446, 740
BstACI	GRCGYC	1 cut(s)	36
BstAPI	GCANNNNNTGC	1 cut(s)	1015
BstC8I	GCNNGC	7 cut(s)	27, 305, 513, 533, 639, 857, 1263
BstDEI	CTNAG	4 cut(s)	136, 141, 1211, 1272
BstDSI	CCRYGG	2 cut(s)	43, 305
BstF5I	GGATG	6 cut(s)	520, 721, 1098, 1231, 1235, 1312
BstFNI	CGCG	3 cut(s)	45, 99, 307
BstKTI	GATC	8 cut(s)	8, 685, 733, 802, 930, 1111, 1150, 1278
BstMAI	GTCTC	3 cut(s)	133, 389, 968
BstMBI	GATC	8 cut(s)	5, 682, 730, 799, 927, 1108, 1147, 1275
BstMCI	CGRYCG	1 cut(s)	271
BstMWI	GCNNNNNNNGC	6 cut(s)	26, 35, 62, 71, 920, 1015
BstNI	CCWGG	2 cut(s)	328, 601
BstSCI	CCNGG	4 cut(s)	326, 599, 859, 1238
BstSFI	CTRYAG	2 cut(s)	677, 969
BstUI	CGCG	3 cut(s)	45, 99, 307
BstV1I	GCAGC	2 cut(s)	205, 769
BstV2I	GAAGAC	3 cut(s)	108, 761, 871
BstX2I	RGATCY	1 cut(s)	682
BstYI	RGATCY	1 cut(s)	682
Bsu15I	ATCGAT	1 cut(s)	733
BsuRI	GGCC	2 cut(s)	303, 948
BsuTUI	ATCGAT	1 cut(s)	733
BtgI	CCRYGG	2 cut(s)	43, 305
BtrI	CACGTC	3 cut(s)	595, 610, 1322
BtsCI	GGATG	6 cut(s)	520, 721, 1098, 1231, 1235, 1312
BtsI	GCAGTG	1 cut(s)	674
BtsIMutI	CAGTG	1 cut(s)	674
Cac8I	GCNNGC	7 cut(s)	27, 305, 513, 533, 639, 857, 1263
CciI	TCATGA	1 cut(s)	937
Cfr13I	GGNCC	2 cut(s)	302, 705
Cfr42I	CCGCGG	2 cut(s)	46, 308
ClaI	ATCGAT	1 cut(s)	733
CseI	GACGC	2 cut(s)	44, 609
Csp6I	GTAC	4 cut(s)	10, 223, 406, 1171
CviAII	CATG	5 cut(s)	590, 644, 938, 950, 1279
CviQI	GTAC	4 cut(s)	10, 223, 406, 1171
DdeI	CTNAG	4 cut(s)	136, 141, 1211, 1272
DpnI	GATC	8 cut(s)	7, 684, 732, 801, 929, 1110, 1149, 1277
DpnII	GATC	8 cut(s)	5, 682, 730, 799, 927, 1108, 1147, 1275
DriI	GACNNNNNGTC	1 cut(s)	619
EaeI	YGGCCR	1 cut(s)	946
Eam1104I	CTCTTC	2 cut(s)	446, 740
Eam1105I	GACNNNNNGTC	1 cut(s)	619
EarI	CTCTTC	2 cut(s)	446, 740
Eco130I	CCWWGG	2 cut(s)	444, 552
Eco24I	GRGCYC	1 cut(s)	90
Eco47I	GGWCC	1 cut(s)	705
EcoRII	CCWGG	2 cut(s)	326, 599
EcoT14I	CCWWGG	2 cut(s)	444, 552
EcoT22I	ATGCAT	1 cut(s)	850
EcoT38I	GRGCYC	1 cut(s)	90
ErhI	CCWWGG	2 cut(s)	444, 552
Esp3I	CGTCTC	2 cut(s)	133, 389
FaeI	CATG	5 cut(s)	593, 647, 941, 953, 1282
FalI	AAGNNNNNCTT	1 cut(s)	1323
FaqI	GGGAC	3 cut(s)	309, 515, 691
FatI	CATG	5 cut(s)	589, 643, 937, 949, 1278
FauI	CCCGC	4 cut(s)	270, 312, 520, 528
FblI	GTMKAC	1 cut(s)	267
Fnu4HI	GCNGC	5 cut(s)	46, 75, 78, 194, 783
FokI	GGATG	6 cut(s)	507, 728, 1105, 1218, 1222, 1319
FriOI	GRGCYC	1 cut(s)	90
Fsp4HI	GCNGC	5 cut(s)	46, 75, 78, 194, 783
FspBI	CTAG	1 cut(s)	249
GluI	GCNGC	5 cut(s)	46, 75, 78, 194, 783
HaeIII	GGCC	2 cut(s)	303, 948
HapII	CCGG	6 cut(s)	271, 524, 624, 689, 860, 1239
HgaI	GACGC	2 cut(s)	44, 609
Hin1I	GRCGYC	1 cut(s)	36
Hin1II	CATG	5 cut(s)	593, 647, 941, 953, 1282
HincII	GTYRAC	2 cut(s)	268, 1048
HindII	GTYRAC	2 cut(s)	268, 1048
HinfI	GANTC	5 cut(s)	204, 322, 400, 721, 1202
HpaII	CCGG	6 cut(s)	271, 524, 624, 689, 860, 1239
HphI	GGTGA	3 cut(s)	275, 364, 1211
Hpy166II	GTNNAC	6 cut(s)	268, 283, 408, 613, 1048, 1171
Hpy188I	TCNGA	1 cut(s)	1214
Hpy188III	TCNNGA	7 cut(s)	239, 524, 689, 725, 938, 1069, 1086
Hpy8I	GTNNAC	6 cut(s)	268, 283, 408, 613, 1048, 1171
Hpy99I	CGWCG	2 cut(s)	38, 928
HpyAV	CCTTC	3 cut(s)	49, 1083, 1325
HpyCH4III	ACNGT	3 cut(s)	222, 376, 1168
HpyCH4IV	ACGT	4 cut(s)	333, 594, 609, 1321
HpyCH4V	TGCA	9 cut(s)	246, 574, 679, 848, 971, 1001, 1009, 1208, 1310
HpyF10VI	GCNNNNNNNGC	6 cut(s)	26, 35, 62, 71, 920, 1015
HpyF3I	CTNAG	4 cut(s)	136, 141, 1211, 1272
HpySE526I	ACGT	4 cut(s)	333, 594, 609, 1321
Hsp92I	GRCGYC	1 cut(s)	36
Hsp92II	CATG	5 cut(s)	593, 647, 941, 953, 1282
Kpn2I	TCCGGA	2 cut(s)	523, 688
KspI	CCGCGG	2 cut(s)	46, 308
Kzo9I	GATC	8 cut(s)	5, 682, 730, 799, 927, 1108, 1147, 1275
LmnI	GCTCC	3 cut(s)	634, 1165, 1313
Lsp1109I	GCAGC	2 cut(s)	205, 769
LweI	GCATC	2 cut(s)	901, 1297
MaeI	CTAG	1 cut(s)	249
MaeII	ACGT	4 cut(s)	333, 594, 609, 1321
MaeIII	GTNAC	3 cut(s)	595, 1030, 1189
MalI	GATC	8 cut(s)	7, 684, 732, 801, 929, 1110, 1149, 1277
MboI	GATC	8 cut(s)	5, 682, 730, 799, 927, 1108, 1147, 1275
MboII	GAAGA	9 cut(s)	108, 150, 159, 463, 757, 766, 871, 880, 1295
MflI	RGATCY	1 cut(s)	682
MhlI	GDGCHC	1 cut(s)	90
MlsI	TGGCCA	1 cut(s)	948
MluNI	TGGCCA	1 cut(s)	948
MlyI	GAGTC	1 cut(s)	331
MmeI	TCCRAC	2 cut(s)	156, 1187
Mox20I	TGGCCA	1 cut(s)	948
Mph1103I	ATGCAT	1 cut(s)	850
MroI	TCCGGA	2 cut(s)	523, 688
MroXI	GAANNNNTTC	1 cut(s)	1335
MscI	TGGCCA	1 cut(s)	948
MslI	CAYNNNNRTG	2 cut(s)	648, 668
Msp20I	TGGCCA	1 cut(s)	948
MspA1I	CMGCKG	2 cut(s)	45, 307
MspI	CCGG	6 cut(s)	271, 524, 624, 689, 860, 1239
MspR9I	CCNGG	4 cut(s)	328, 601, 861, 1240
MvaI	CCWGG	2 cut(s)	328, 601
MvnI	CGCG	3 cut(s)	45, 99, 307
MwoI	GCNNNNNNNGC	6 cut(s)	26, 35, 62, 71, 920, 1015
NciI	CCSGG	2 cut(s)	861, 1240
NdeII	GATC	8 cut(s)	5, 682, 730, 799, 927, 1108, 1147, 1275
NlaIII	CATG	5 cut(s)	593, 647, 941, 953, 1282
NlaIV	GGNNCC	3 cut(s)	684, 707, 1161
NmeAIII	GCCGAG	2 cut(s)	321, 940
NmuCI	GTSAC	1 cut(s)	595
NsiI	ATGCAT	1 cut(s)	850
PagI	TCATGA	1 cut(s)	937
PdmI	GAANNNNTTC	1 cut(s)	1335
PfeI	GAWTC	4 cut(s)	204, 400, 721, 1202
PflMI	CCANNNNNTGG	1 cut(s)	955
PfoI	TCCNGGA	1 cut(s)	1238
PkrI	GCNGC	5 cut(s)	47, 76, 79, 195, 784
PleI	GAGTC	1 cut(s)	330
PpsI	GAGTC	1 cut(s)	330
PsiI	TTATAA	1 cut(s)	428
Psp1406I	AACGTT	1 cut(s)	333
Psp6I	CCWGG	2 cut(s)	326, 599
PspGI	CCWGG	2 cut(s)	326, 599
PspN4I	GGNNCC	3 cut(s)	684, 707, 1161
PspPI	GGNCC	2 cut(s)	302, 705
PstI	CTGCAG	2 cut(s)	681, 973
PsuI	RGATCY	1 cut(s)	682
RsaI	GTAC	4 cut(s)	11, 224, 407, 1172
RsaNI	GTAC	4 cut(s)	10, 223, 406, 1171
RseI	CAYNNNNRTG	2 cut(s)	648, 668
SacII	CCGCGG	2 cut(s)	46, 308
SalI	GTCGAC	1 cut(s)	266
SatI	GCNGC	5 cut(s)	46, 75, 78, 194, 783
Sau3AI	GATC	8 cut(s)	5, 682, 730, 799, 927, 1108, 1147, 1275
Sau96I	GGNCC	2 cut(s)	302, 705
SchI	GAGTC	1 cut(s)	331
ScrFI	CCNGG	4 cut(s)	328, 601, 861, 1240
SduI	GDGCHC	1 cut(s)	90
SfaNI	GCATC	2 cut(s)	901, 1297
SfcI	CTRYAG	2 cut(s)	677, 969
Sfr303I	CCGCGG	2 cut(s)	46, 308
SgrBI	CCGCGG	2 cut(s)	46, 308
SinI	GGWCC	1 cut(s)	705
SmiMI	CAYNNNNRTG	2 cut(s)	648, 668
SmlI	CTYRAG	3 cut(s)	893, 1069, 1266
SmoI	CTYRAG	3 cut(s)	893, 1069, 1266
SspMI	CTAG	1 cut(s)	249
StyD4I	CCNGG	4 cut(s)	326, 599, 859, 1238
StyI	CCWWGG	2 cut(s)	444, 552
TaaI	ACNGT	3 cut(s)	222, 376, 1168
TaiI	ACGT	4 cut(s)	336, 597, 612, 1324
TaqI	TCGA	5 cut(s)	33, 267, 437, 733, 961
TatI	WGTACW	1 cut(s)	222
TauI	GCSGC	3 cut(s)	48, 77, 80
TfiI	GAWTC	4 cut(s)	204, 400, 721, 1202
TscAI	CASTG	1 cut(s)	681
TseFI	GTSAC	1 cut(s)	595
TseI	GCWGC	2 cut(s)	193, 782
Tsp45I	GTSAC	1 cut(s)	595
TspDTI	ATGAA	1 cut(s)	497
TspGWI	ACGGA	2 cut(s)	115, 393
TspRI	CASTG	1 cut(s)	681
Van91I	CCANNNNNTGG	1 cut(s)	955
VpaK11BI	GGWCC	1 cut(s)	705
XapI	RAATTY	1 cut(s)	388
XcmI	CCANNNNNNNNNTGG	1 cut(s)	793
XmiI	GTMKAC	1 cut(s)	267
XmnI	GAANNNNTTC	1 cut(s)	1335
XspI	CTAG	1 cut(s)	249
Zsp2I	ATGCAT	1 cut(s)	850

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.