MD07G1268900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0006160
Physical Location & Seq
Forward (+)
33650424 .. 33650726
303 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1268900.v1.1.491

Sequence Viewer

Length: 303 bp
ATGAAGGTCCGGGTGGTGTGTTGGAAAGTGTATGATTACATTCGTTATGATCTGAAAGAGATTGCATTTCCATCTTCGCTGCCGGACCCTTCTCATATCAAGAAGCGGCGTCAGCTCACCATGCGGGAATGGTTTTTGGTGTTAAAGAAGGCTTCTAGGCTTTATGCCGCCAGCTGGGTGCGGGATGTTGGTCATGAACTGCGACCTAATGATTATAAGAATAAAAAGAGTGAAGATGGACCTGGTGGAGCACAGAGAACGGCTAAAGAGAAAGAACCCTCAACCTTAGAGGATCTTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

101

Amino Acids

11.74

Weight (kDa)

9.64

Isoelectric Point (pI)

53.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 216
AciI CCGC 4 cut(s) 106, 124, 168, 181
AclWI GGATC 1 cut(s) 300
AcyI GRCGYC 1 cut(s) 109
AfiI CCNNNNNNNGG 1 cut(s) 174
AjnI CCWGG 1 cut(s) 241
AluBI AGCT 2 cut(s) 115, 174
AluI AGCT 2 cut(s) 115, 174
Alw21I GWGCWC 1 cut(s) 253
AlwI GGATC 1 cut(s) 300
ApeKI GCWGC 1 cut(s) 79
AspS9I GGNCC 3 cut(s) 7, 85, 239
AsuC2I CCSGG 1 cut(s) 11
AsuHPI GGTGA 1 cut(s) 109
AvaII GGWCC 3 cut(s) 7, 85, 239
Bbv12I GWGCWC 1 cut(s) 253
BbvI GCAGC 1 cut(s) 66
BccI CCATC 2 cut(s) 79, 230
BceAI ACGGC 1 cut(s) 276
BciT130I CCWGG 1 cut(s) 243
BcnI CCSGG 1 cut(s) 11
BfaI CTAG 1 cut(s) 156
BisI GCNGC 3 cut(s) 80, 107, 168
BlsI GCNGC 3 cut(s) 81, 108, 169
Bme1390I CCNGG 2 cut(s) 11, 243
Bme18I GGWCC 3 cut(s) 7, 85, 239
BmgT120I GGNCC 3 cut(s) 7, 85, 239
BmiI GGNNCC 1 cut(s) 87
BmrFI CCNGG 2 cut(s) 11, 243
BpuMI CCSGG 1 cut(s) 11
BsaHI GRCGYC 1 cut(s) 109
Bsc4I CCNNNNNNNGG 1 cut(s) 174
BseBI CCWGG 1 cut(s) 243
BseGI GGATG 1 cut(s) 190
BseLI CCNNNNNNNGG 1 cut(s) 174
BseXI GCAGC 1 cut(s) 66
BseYI CCCAGC 1 cut(s) 174
BsiHKAI GWGCWC 1 cut(s) 253
BsiSI CCGG 2 cut(s) 10, 83
BslI CCNNNNNNNGG 1 cut(s) 174
Bsp1286I GDGCHC 1 cut(s) 253
Bsp143I GATC 2 cut(s) 49, 292
BspACI CCGC 4 cut(s) 106, 124, 168, 181
BspHI TCATGA 1 cut(s) 193
BspLI GGNNCC 1 cut(s) 87
BspPI GGATC 1 cut(s) 300
BssMI GATC 2 cut(s) 49, 292
BssNI GRCGYC 1 cut(s) 109
Bst2UI CCWGG 1 cut(s) 243
BstACI GRCGYC 1 cut(s) 109
BstC8I GCNNGC 1 cut(s) 172
BstDEI CTNAG 1 cut(s) 286
BstF5I GGATG 1 cut(s) 190
BstKTI GATC 2 cut(s) 52, 295
BstMBI GATC 2 cut(s) 49, 292
BstMWI GCNNNNNNNGC 2 cut(s) 112, 121
BstNI CCWGG 1 cut(s) 243
BstSCI CCNGG 2 cut(s) 9, 241
BstV1I GCAGC 1 cut(s) 66
BstX2I RGATCY 1 cut(s) 292
BstYI RGATCY 1 cut(s) 292
BtsCI GGATG 1 cut(s) 190
Cac8I GCNNGC 1 cut(s) 172
CciI TCATGA 1 cut(s) 193
Cfr13I GGNCC 3 cut(s) 7, 85, 239
CseI GACGC 1 cut(s) 98
CsiI ACCWGGT 1 cut(s) 241
CviAII CATG 2 cut(s) 121, 194
CviJI RGCY 5 cut(s) 115, 152, 160, 174, 263
CviKI_1 RGCY 5 cut(s) 115, 152, 160, 174, 263
DdeI CTNAG 1 cut(s) 286
DpnI GATC 2 cut(s) 51, 294
DpnII GATC 2 cut(s) 49, 292
Eco47I GGWCC 3 cut(s) 7, 85, 239
EcoRII CCWGG 1 cut(s) 241
FaeI CATG 2 cut(s) 124, 197
FaiI YATR 7 cut(s) 33, 48, 96, 122, 165, 195, 216
FatI CATG 2 cut(s) 120, 193
FauI CCCGC 2 cut(s) 117, 174
Fnu4HI GCNGC 3 cut(s) 80, 107, 168
FokI GGATG 1 cut(s) 197
Fsp4HI GCNGC 3 cut(s) 80, 107, 168
FspBI CTAG 1 cut(s) 156
GluI GCNGC 3 cut(s) 80, 107, 168
GsaI CCCAGC 1 cut(s) 178
HapII CCGG 2 cut(s) 10, 83
HgaI GACGC 1 cut(s) 98
Hin1I GRCGYC 1 cut(s) 109
Hin1II CATG 2 cut(s) 124, 197
HpaII CCGG 2 cut(s) 10, 83
HphI GGTGA 1 cut(s) 109
Hpy188I TCNGA 1 cut(s) 54
Hpy188III TCNNGA 2 cut(s) 100, 194
HpyAV CCTTC 2 cut(s) 99, 142
HpyCH4V TGCA 1 cut(s) 65
HpyF10VI GCNNNNNNNGC 2 cut(s) 112, 121
HpyF3I CTNAG 1 cut(s) 286
Hsp92I GRCGYC 1 cut(s) 109
Hsp92II CATG 2 cut(s) 124, 197
Kzo9I GATC 2 cut(s) 49, 292
LmnI GCTCC 1 cut(s) 248
LpnPI CCDG 6 cut(s) 23, 96, 160, 184, 228, 255
Lsp1109I GCAGC 1 cut(s) 66
MabI ACCWGGT 1 cut(s) 241
MaeI CTAG 1 cut(s) 156
MalI GATC 2 cut(s) 51, 294
MboI GATC 2 cut(s) 49, 292
MboII GAAGA 2 cut(s) 66, 245
MflI RGATCY 1 cut(s) 292
MhlI GDGCHC 1 cut(s) 253
MnlI CCTC 2 cut(s) 283, 289
MseI TTAA 1 cut(s) 143
MspA1I CMGCKG 1 cut(s) 174
MspI CCGG 2 cut(s) 10, 83
MspR9I CCNGG 2 cut(s) 11, 243
MvaI CCWGG 1 cut(s) 243
MwoI GCNNNNNNNGC 2 cut(s) 112, 121
NciI CCSGG 1 cut(s) 11
NdeII GATC 2 cut(s) 49, 292
NlaIII CATG 2 cut(s) 124, 197
NlaIV GGNNCC 1 cut(s) 87
PagI TCATGA 1 cut(s) 193
PkrI GCNGC 3 cut(s) 81, 108, 169
PsiI TTATAA 1 cut(s) 216
Psp6I CCWGG 1 cut(s) 241
PspFI CCCAGC 1 cut(s) 174
PspGI CCWGG 1 cut(s) 241
PspN4I GGNNCC 1 cut(s) 87
PspPI GGNCC 3 cut(s) 7, 85, 239
PsuI RGATCY 1 cut(s) 292
PvuII CAGCTG 1 cut(s) 174
SaqAI TTAA 1 cut(s) 143
SatI GCNGC 3 cut(s) 80, 107, 168
Sau3AI GATC 2 cut(s) 49, 292
Sau96I GGNCC 3 cut(s) 7, 85, 239
ScrFI CCNGG 2 cut(s) 11, 243
SduI GDGCHC 1 cut(s) 253
SetI ASST 6 cut(s) 9, 117, 176, 208, 244, 287
SexAI ACCWGGT 1 cut(s) 241
SinI GGWCC 3 cut(s) 7, 85, 239
SsiI CCGC 4 cut(s) 106, 124, 168, 181
SspMI CTAG 1 cut(s) 156
StyD4I CCNGG 2 cut(s) 9, 241
TauI GCSGC 2 cut(s) 109, 170
Tru1I TTAA 1 cut(s) 143
Tru9I TTAA 1 cut(s) 143
TseI GCWGC 1 cut(s) 79
TspDTI ATGAA 2 cut(s) 17, 210
VpaK11BI GGWCC 3 cut(s) 7, 85, 239
XspI CTAG 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.