pycom05g04320

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0006160
Physical Location & Seq
Forward (+)
5055480 .. 5057489
2010 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g04320.2

Sequence Viewer

Length: 486 bp
ATGAAGGTCAGGGTAGTGTGTCGGAAAGTGTGTGATTACATTCGTTATGATCTGAAAGAGATTGCATTCCCATCTTCGCTGCCGGACCCTCCTCATATAAAGAAGCGTCGTAAGCTCACCTGGCATGAGCGGTTCTTGGTGTTGAAGGAGGCTTCTAGGCTTTATGCCGCCAGCTGGGTGCGGGATGTTGGTCCTGAACTGCGACCTAATGATTATAAGAATGAAGAGAGTGAAGATAGACCTGGTGGAGCACAAAGAACGGCTAAGGGGAAAGAACCCTCAACATTAGAGGATCTTGCTGTGGCCGCAAGAGGAGGAATGGAGATGCTAAAGCCTGCTCTACAGCGCGTGTACATGACAAGAGCTTCTGCATATCGGGATGCTCTTCAAAGTTTTATACATGGGTACCAAGAAGGCATCCAGCAGGTCATGGAGAAAAAGGCAAAAGATTCTAAATCTCAACAAGAAAGTGATAAATCTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

162

Amino Acids

18.51

Weight (kDa)

9.49

Isoelectric Point (pI)

53.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 216
Acc36I ACCTGC 1 cut(s) 415
Acc65I GGTACC 1 cut(s) 405
AccB1I GGYRCC 1 cut(s) 405
AccBSI CCGCTC 1 cut(s) 130
AccII CGCG 1 cut(s) 348
AciI CCGC 4 cut(s) 130, 168, 181, 306
AclWI GGATC 1 cut(s) 300
AcoI YGGCCR 1 cut(s) 303
AfaI GTAC 2 cut(s) 353, 407
AfiI CCNNNNNNNGG 1 cut(s) 174
AgsI TTSAA 2 cut(s) 145, 389
AjnI CCWGG 2 cut(s) 119, 241
AluBI AGCT 3 cut(s) 115, 174, 365
AluI AGCT 3 cut(s) 115, 174, 365
Alw21I GWGCWC 1 cut(s) 253
AlwI GGATC 1 cut(s) 300
AoxI GGCC 1 cut(s) 303
ApeKI GCWGC 1 cut(s) 79
Asp718I GGTACC 1 cut(s) 405
AspLEI GCGC 1 cut(s) 348
AspS9I GGNCC 2 cut(s) 85, 191
AsuHPI GGTGA 1 cut(s) 109
AvaII GGWCC 2 cut(s) 85, 191
BaeI ACNNNNGTAYC 2 cut(s) 389, 422
BanI GGYRCC 1 cut(s) 405
Bbv12I GWGCWC 1 cut(s) 253
BbvI GCAGC 1 cut(s) 66
BccI CCATC 1 cut(s) 79
BceAI ACGGC 1 cut(s) 276
BciT130I CCWGG 2 cut(s) 121, 243
BfaI CTAG 1 cut(s) 156
BfmI CTRYAG 1 cut(s) 341
BfuAI ACCTGC 1 cut(s) 415
BisI GCNGC 3 cut(s) 80, 168, 306
BlsI GCNGC 3 cut(s) 81, 169, 307
Bme1390I CCNGG 2 cut(s) 121, 243
Bme18I GGWCC 2 cut(s) 85, 191
BmgT120I GGNCC 2 cut(s) 85, 191
BmiI GGNNCC 2 cut(s) 87, 407
BmrFI CCNGG 2 cut(s) 121, 243
BmsI GCATC 3 cut(s) 315, 370, 426
Bpu10I CCTNAGC 1 cut(s) 264
Bsc4I CCNNNNNNNGG 1 cut(s) 174
BseBI CCWGG 2 cut(s) 121, 243
BseGI GGATG 3 cut(s) 190, 385, 417
BseLI CCNNNNNNNGG 1 cut(s) 174
BseRI GAGGAG 2 cut(s) 81, 327
BseXI GCAGC 1 cut(s) 66
BseYI CCCAGC 1 cut(s) 174
Bsh1236I CGCG 1 cut(s) 348
BshFI GGCC 1 cut(s) 305
BshNI GGYRCC 1 cut(s) 405
BsiHKAI GWGCWC 1 cut(s) 253
BsiSI CCGG 1 cut(s) 83
BslI CCNNNNNNNGG 1 cut(s) 174
BsmI GAATGC 1 cut(s) 65
BsnI GGCC 1 cut(s) 305
Bsp1286I GDGCHC 1 cut(s) 253
Bsp1407I TGTACA 1 cut(s) 351
Bsp143I GATC 2 cut(s) 49, 292
BspACI CCGC 4 cut(s) 130, 168, 181, 306
BspANI GGCC 1 cut(s) 305
BspFNI CGCG 1 cut(s) 348
BspLI GGNNCC 2 cut(s) 87, 407
BspMI ACCTGC 1 cut(s) 415
BspPI GGATC 1 cut(s) 300
BspQI GCTCTTC 1 cut(s) 390
BspT107I GGYRCC 1 cut(s) 405
BsrBI CCGCTC 1 cut(s) 130
BsrGI TGTACA 1 cut(s) 351
BssMI GATC 2 cut(s) 49, 292
Bst2UI CCWGG 2 cut(s) 121, 243
Bst6I CTCTTC 2 cut(s) 219, 390
BstAUI TGTACA 1 cut(s) 351
BstC8I GCNNGC 2 cut(s) 172, 336
BstDEI CTNAG 1 cut(s) 264
BstF5I GGATG 3 cut(s) 190, 385, 417
BstFNI CGCG 1 cut(s) 348
BstHHI GCGC 1 cut(s) 348
BstKTI GATC 2 cut(s) 52, 295
BstMBI GATC 2 cut(s) 49, 292
BstMWI GCNNNNNNNGC 3 cut(s) 112, 121, 305
BstNI CCWGG 2 cut(s) 121, 243
BstSCI CCNGG 2 cut(s) 119, 241
BstSFI CTRYAG 1 cut(s) 341
BstUI CGCG 1 cut(s) 348
BstV1I GCAGC 1 cut(s) 66
BstX2I RGATCY 1 cut(s) 292
BstYI RGATCY 1 cut(s) 292
BsuRI GGCC 1 cut(s) 305
BtsCI GGATG 3 cut(s) 190, 385, 417
BveI ACCTGC 1 cut(s) 415
Cac8I GCNNGC 2 cut(s) 172, 336
CfoI GCGC 1 cut(s) 348
Cfr13I GGNCC 2 cut(s) 85, 191
CseI GACGC 1 cut(s) 95
CsiI ACCWGGT 1 cut(s) 241
Csp6I GTAC 2 cut(s) 352, 406
CviAII CATG 4 cut(s) 125, 355, 401, 430
CviJI RGCY 8 cut(s) 115, 152, 160, 174, 263, 305, 334, 365
CviKI_1 RGCY 8 cut(s) 115, 152, 160, 174, 263, 305, 334, 365
CviQI GTAC 2 cut(s) 352, 406
DdeI CTNAG 1 cut(s) 264
DpnI GATC 2 cut(s) 51, 294
DpnII GATC 2 cut(s) 49, 292
EaeI YGGCCR 1 cut(s) 303
Eam1104I CTCTTC 2 cut(s) 219, 390
EarI CTCTTC 2 cut(s) 219, 390
Eco47I GGWCC 2 cut(s) 85, 191
EcoRII CCWGG 2 cut(s) 119, 241
FaeI CATG 4 cut(s) 128, 358, 404, 433
FatI CATG 4 cut(s) 124, 354, 400, 429
FauI CCCGC 1 cut(s) 174
Fnu4HI GCNGC 3 cut(s) 80, 168, 306
FokI GGATG 3 cut(s) 197, 392, 404
Fsp4HI GCNGC 3 cut(s) 80, 168, 306
FspBI CTAG 1 cut(s) 156
GlaI GCGC 1 cut(s) 347
GluI GCNGC 3 cut(s) 80, 168, 306
GsaI CCCAGC 1 cut(s) 178
HaeIII GGCC 1 cut(s) 305
HapII CCGG 1 cut(s) 83
HgaI GACGC 1 cut(s) 95
HhaI GCGC 1 cut(s) 348
Hin1II CATG 4 cut(s) 128, 358, 404, 433
Hin6I GCGC 1 cut(s) 346
HinP1I GCGC 1 cut(s) 346
HinfI GANTC 1 cut(s) 449
HpaII CCGG 1 cut(s) 83
HphI GGTGA 1 cut(s) 109
Hpy166II GTNNAC 1 cut(s) 352
Hpy188I TCNGA 2 cut(s) 24, 54
Hpy188III TCNNGA 2 cut(s) 194, 377
Hpy8I GTNNAC 1 cut(s) 352
Hpy99I CGWCG 1 cut(s) 111
HpyAV CCTTC 2 cut(s) 139, 407
HpyCH4V TGCA 2 cut(s) 65, 371
HpyF10VI GCNNNNNNNGC 3 cut(s) 112, 121, 305
HpyF3I CTNAG 1 cut(s) 264
Hsp92II CATG 4 cut(s) 128, 358, 404, 433
HspAI GCGC 1 cut(s) 346
KpnI GGTACC 1 cut(s) 409
Kzo9I GATC 2 cut(s) 49, 292
LguI GCTCTTC 1 cut(s) 390
LmnI GCTCC 1 cut(s) 248
Lsp1109I GCAGC 1 cut(s) 66
LweI GCATC 3 cut(s) 315, 370, 426
MabI ACCWGGT 1 cut(s) 241
MaeI CTAG 1 cut(s) 156
MalI GATC 2 cut(s) 51, 294
MbiI CCGCTC 1 cut(s) 130
MboI GATC 2 cut(s) 49, 292
MboII GAAGA 4 cut(s) 66, 236, 245, 377
MflI RGATCY 1 cut(s) 292
MhlI GDGCHC 1 cut(s) 253
MnlI CCTC 7 cut(s) 99, 102, 142, 283, 289, 305, 308
MseI TTAA 1 cut(s) 484
MspA1I CMGCKG 1 cut(s) 174
MspI CCGG 1 cut(s) 83
MspR9I CCNGG 2 cut(s) 121, 243
Mva1269I GAATGC 1 cut(s) 65
MvaI CCWGG 2 cut(s) 121, 243
MvnI CGCG 1 cut(s) 348
MwoI GCNNNNNNNGC 3 cut(s) 112, 121, 305
NdeII GATC 2 cut(s) 49, 292
NlaIII CATG 4 cut(s) 128, 358, 404, 433
NlaIV GGNNCC 2 cut(s) 87, 407
PciSI GCTCTTC 1 cut(s) 390
PctI GAATGC 1 cut(s) 65
PfeI GAWTC 1 cut(s) 449
PkrI GCNGC 3 cut(s) 81, 169, 307
PsiI TTATAA 1 cut(s) 216
Psp6I CCWGG 2 cut(s) 119, 241
PspFI CCCAGC 1 cut(s) 174
PspGI CCWGG 2 cut(s) 119, 241
PspN4I GGNNCC 2 cut(s) 87, 407
PspPI GGNCC 2 cut(s) 85, 191
PsuI RGATCY 1 cut(s) 292
PvuII CAGCTG 1 cut(s) 174
RsaI GTAC 2 cut(s) 353, 407
RsaNI GTAC 2 cut(s) 352, 406
SapI GCTCTTC 1 cut(s) 390
SaqAI TTAA 1 cut(s) 484
SatI GCNGC 3 cut(s) 80, 168, 306
Sau3AI GATC 2 cut(s) 49, 292
Sau96I GGNCC 2 cut(s) 85, 191
ScrFI CCNGG 2 cut(s) 121, 243
SduI GDGCHC 1 cut(s) 253
SetI ASST 8 cut(s) 9, 117, 122, 176, 208, 244, 367, 429
SexAI ACCWGGT 1 cut(s) 241
SfaNI GCATC 3 cut(s) 315, 370, 426
SfcI CTRYAG 1 cut(s) 341
SinI GGWCC 2 cut(s) 85, 191
SsiI CCGC 4 cut(s) 130, 168, 181, 306
SspMI CTAG 1 cut(s) 156
StyD4I CCNGG 2 cut(s) 119, 241
TatI WGTACW 1 cut(s) 351
TauI GCSGC 2 cut(s) 170, 308
TfiI GAWTC 1 cut(s) 449
Tru1I TTAA 1 cut(s) 484
Tru9I TTAA 1 cut(s) 484
TseI GCWGC 1 cut(s) 79
TspDTI ATGAA 2 cut(s) 17, 237
VpaK11BI GGWCC 2 cut(s) 85, 191
XspI CTAG 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.