MD10G1058500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006160
Physical Location & Seq
Forward (+)
7909545 .. 7912802
3258 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1058500.v1.1.491

Sequence Viewer

Length: 486 bp
ATGAAGGTCCGGGTGGTGTGTCGGAAAGTGTATGATTACATTCGTTATGATCTGAAAGAGATTGCATTTCCATCTTCGCTGCCGGACCCTCCTCATATCAAGAAGCGCCGTAAGCTCACCATGCGTGAGTGGTTCTTGGTGTTGAAGGAGGCTTCTAGGCTTTATGCCGCCAGCTGGGTGCGGGATGTTGGTCCTGAACTGCGACCTAATGATTATAAGAATAAAGAGAGTGAAGATGGACCTGGTGGAGCACAGAGAACGGCTAAAGAGAAAGAACCCTCAACTTTAGAGGATCTTGCTGTGGCTGCAAGAGGAGGAATGGAGACATTAAAGCCTGCTCTGCAGCGGGTGTACATGACAAGAGCGTCTGCATATAGAGATGCTCTTCAAAGTTTTATACATGGGTACCAAGAAGGCATCCAGCAGGTCATGGAGAAAAAGGCAAAAGATTCTAAATCTGAACAAGAAAGTGATAAATCTGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

162

Amino Acids

18.48

Weight (kDa)

9.42

Isoelectric Point (pI)

51.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 216
AasI GACNNNNNNGTC 1 cut(s) 364
Acc36I ACCTGC 1 cut(s) 415
Acc65I GGTACC 1 cut(s) 405
AccB1I GGYRCC 1 cut(s) 405
AciI CCGC 3 cut(s) 168, 181, 346
AclWI GGATC 1 cut(s) 300
AfaI GTAC 2 cut(s) 353, 407
AfiI CCNNNNNNNGG 1 cut(s) 174
AgsI TTSAA 2 cut(s) 145, 389
AjnI CCWGG 1 cut(s) 241
AluBI AGCT 2 cut(s) 115, 174
AluI AGCT 2 cut(s) 115, 174
Alw21I GWGCWC 1 cut(s) 253
Alw26I GTCTC 1 cut(s) 317
AlwI GGATC 1 cut(s) 300
ApeKI GCWGC 3 cut(s) 79, 305, 343
Asp718I GGTACC 1 cut(s) 405
AspLEI GCGC 1 cut(s) 108
AspS9I GGNCC 4 cut(s) 7, 85, 191, 239
AsuC2I CCSGG 1 cut(s) 11
AsuHPI GGTGA 1 cut(s) 109
AvaII GGWCC 4 cut(s) 7, 85, 191, 239
BaeI ACNNNNGTAYC 2 cut(s) 389, 422
BanI GGYRCC 1 cut(s) 405
Bbv12I GWGCWC 1 cut(s) 253
BbvI GCAGC 3 cut(s) 66, 292, 355
BccI CCATC 2 cut(s) 79, 230
BceAI ACGGC 2 cut(s) 93, 276
BciT130I CCWGG 1 cut(s) 243
BcnI CCSGG 1 cut(s) 11
BcoDI GTCTC 1 cut(s) 317
BfaI CTAG 1 cut(s) 156
BfmI CTRYAG 1 cut(s) 341
BfoI RGCGCY 1 cut(s) 109
BfuAI ACCTGC 1 cut(s) 415
BisI GCNGC 4 cut(s) 80, 168, 306, 344
BlsI GCNGC 4 cut(s) 81, 169, 307, 345
Bme1390I CCNGG 2 cut(s) 11, 243
Bme18I GGWCC 4 cut(s) 7, 85, 191, 239
BmgT120I GGNCC 4 cut(s) 7, 85, 191, 239
BmiI GGNNCC 2 cut(s) 87, 407
BmrFI CCNGG 2 cut(s) 11, 243
BmsI GCATC 2 cut(s) 370, 426
BpuMI CCSGG 1 cut(s) 11
Bsc4I CCNNNNNNNGG 1 cut(s) 174
BseBI CCWGG 1 cut(s) 243
BseGI GGATG 2 cut(s) 190, 417
BseLI CCNNNNNNNGG 1 cut(s) 174
BseRI GAGGAG 2 cut(s) 81, 327
BseXI GCAGC 3 cut(s) 66, 292, 355
BseYI CCCAGC 1 cut(s) 174
BshNI GGYRCC 1 cut(s) 405
BsiHKAI GWGCWC 1 cut(s) 253
BsiSI CCGG 2 cut(s) 10, 83
BslI CCNNNNNNNGG 1 cut(s) 174
BsmAI GTCTC 1 cut(s) 317
Bsp1286I GDGCHC 1 cut(s) 253
Bsp1407I TGTACA 1 cut(s) 351
Bsp143I GATC 2 cut(s) 49, 292
BspACI CCGC 3 cut(s) 168, 181, 346
BspLI GGNNCC 2 cut(s) 87, 407
BspMAI CTGCAG 1 cut(s) 345
BspMI ACCTGC 1 cut(s) 415
BspPI GGATC 1 cut(s) 300
BspQI GCTCTTC 1 cut(s) 390
BspT107I GGYRCC 1 cut(s) 405
BsrGI TGTACA 1 cut(s) 351
BssMI GATC 2 cut(s) 49, 292
Bst2UI CCWGG 1 cut(s) 243
Bst6I CTCTTC 1 cut(s) 390
BstAUI TGTACA 1 cut(s) 351
BstC8I GCNNGC 2 cut(s) 172, 336
BstF5I GGATG 2 cut(s) 190, 417
BstH2I RGCGCY 1 cut(s) 109
BstHHI GCGC 1 cut(s) 108
BstKTI GATC 2 cut(s) 52, 295
BstMAI GTCTC 1 cut(s) 317
BstMBI GATC 2 cut(s) 49, 292
BstMWI GCNNNNNNNGC 4 cut(s) 112, 121, 305, 340
BstNI CCWGG 1 cut(s) 243
BstSCI CCNGG 2 cut(s) 9, 241
BstSFI CTRYAG 1 cut(s) 341
BstV1I GCAGC 3 cut(s) 66, 292, 355
BstX2I RGATCY 1 cut(s) 292
BstYI RGATCY 1 cut(s) 292
BtsCI GGATG 2 cut(s) 190, 417
BveI ACCTGC 1 cut(s) 415
Cac8I GCNNGC 2 cut(s) 172, 336
CfoI GCGC 1 cut(s) 108
Cfr13I GGNCC 4 cut(s) 7, 85, 191, 239
CseI GACGC 1 cut(s) 354
CsiI ACCWGGT 1 cut(s) 241
Csp6I GTAC 2 cut(s) 352, 406
CviAII CATG 4 cut(s) 121, 355, 401, 430
CviJI RGCY 7 cut(s) 115, 152, 160, 174, 263, 305, 334
CviKI_1 RGCY 7 cut(s) 115, 152, 160, 174, 263, 305, 334
CviQI GTAC 2 cut(s) 352, 406
DpnI GATC 2 cut(s) 51, 294
DpnII GATC 2 cut(s) 49, 292
DrdI GACNNNNNNGTC 1 cut(s) 364
DseDI GACNNNNNNGTC 1 cut(s) 364
Eam1104I CTCTTC 1 cut(s) 390
EarI CTCTTC 1 cut(s) 390
Eco47I GGWCC 4 cut(s) 7, 85, 191, 239
EcoRII CCWGG 1 cut(s) 241
FaeI CATG 4 cut(s) 124, 358, 404, 433
FatI CATG 4 cut(s) 120, 354, 400, 429
FauI CCCGC 2 cut(s) 174, 339
Fnu4HI GCNGC 4 cut(s) 80, 168, 306, 344
FokI GGATG 2 cut(s) 197, 404
Fsp4HI GCNGC 4 cut(s) 80, 168, 306, 344
FspBI CTAG 1 cut(s) 156
GlaI GCGC 1 cut(s) 107
GluI GCNGC 4 cut(s) 80, 168, 306, 344
GsaI CCCAGC 1 cut(s) 178
HaeII RGCGCY 1 cut(s) 109
HapII CCGG 2 cut(s) 10, 83
HgaI GACGC 1 cut(s) 354
HhaI GCGC 1 cut(s) 108
Hin1II CATG 4 cut(s) 124, 358, 404, 433
Hin6I GCGC 1 cut(s) 106
HinP1I GCGC 1 cut(s) 106
HinfI GANTC 1 cut(s) 449
HpaII CCGG 2 cut(s) 10, 83
HphI GGTGA 1 cut(s) 109
Hpy166II GTNNAC 1 cut(s) 352
Hpy188I TCNGA 3 cut(s) 24, 54, 460
Hpy188III TCNNGA 2 cut(s) 100, 194
Hpy8I GTNNAC 1 cut(s) 352
HpyAV CCTTC 2 cut(s) 139, 407
HpyCH4V TGCA 4 cut(s) 65, 308, 343, 371
HpyF10VI GCNNNNNNNGC 4 cut(s) 112, 121, 305, 340
Hsp92II CATG 4 cut(s) 124, 358, 404, 433
HspAI GCGC 1 cut(s) 106
KpnI GGTACC 1 cut(s) 409
Kzo9I GATC 2 cut(s) 49, 292
LguI GCTCTTC 1 cut(s) 390
LmnI GCTCC 1 cut(s) 248
Lsp1109I GCAGC 3 cut(s) 66, 292, 355
LweI GCATC 2 cut(s) 370, 426
MabI ACCWGGT 1 cut(s) 241
MaeI CTAG 1 cut(s) 156
MalI GATC 2 cut(s) 51, 294
MboI GATC 2 cut(s) 49, 292
MboII GAAGA 3 cut(s) 66, 245, 377
MflI RGATCY 1 cut(s) 292
MhlI GDGCHC 1 cut(s) 253
MnlI CCTC 7 cut(s) 99, 102, 142, 283, 289, 305, 308
MseI TTAA 1 cut(s) 329
MspA1I CMGCKG 2 cut(s) 174, 346
MspI CCGG 2 cut(s) 10, 83
MspR9I CCNGG 2 cut(s) 11, 243
MvaI CCWGG 1 cut(s) 243
MwoI GCNNNNNNNGC 4 cut(s) 112, 121, 305, 340
NciI CCSGG 1 cut(s) 11
NdeII GATC 2 cut(s) 49, 292
NlaIII CATG 4 cut(s) 124, 358, 404, 433
NlaIV GGNNCC 2 cut(s) 87, 407
PciSI GCTCTTC 1 cut(s) 390
PfeI GAWTC 1 cut(s) 449
PkrI GCNGC 4 cut(s) 81, 169, 307, 345
PsiI TTATAA 1 cut(s) 216
Psp6I CCWGG 1 cut(s) 241
PspFI CCCAGC 1 cut(s) 174
PspGI CCWGG 1 cut(s) 241
PspN4I GGNNCC 2 cut(s) 87, 407
PspPI GGNCC 4 cut(s) 7, 85, 191, 239
PstI CTGCAG 1 cut(s) 345
PsuI RGATCY 1 cut(s) 292
PvuII CAGCTG 1 cut(s) 174
RsaI GTAC 2 cut(s) 353, 407
RsaNI GTAC 2 cut(s) 352, 406
SapI GCTCTTC 1 cut(s) 390
SaqAI TTAA 1 cut(s) 329
SatI GCNGC 4 cut(s) 80, 168, 306, 344
Sau3AI GATC 2 cut(s) 49, 292
Sau96I GGNCC 4 cut(s) 7, 85, 191, 239
ScrFI CCNGG 2 cut(s) 11, 243
SduI GDGCHC 1 cut(s) 253
SetI ASST 6 cut(s) 9, 117, 176, 208, 244, 429
SexAI ACCWGGT 1 cut(s) 241
SfaNI GCATC 2 cut(s) 370, 426
SfcI CTRYAG 1 cut(s) 341
SinI GGWCC 4 cut(s) 7, 85, 191, 239
SsiI CCGC 3 cut(s) 168, 181, 346
SspMI CTAG 1 cut(s) 156
StyD4I CCNGG 2 cut(s) 9, 241
TatI WGTACW 1 cut(s) 351
TauI GCSGC 1 cut(s) 170
TfiI GAWTC 1 cut(s) 449
Tru1I TTAA 1 cut(s) 329
Tru9I TTAA 1 cut(s) 329
TseI GCWGC 3 cut(s) 79, 305, 343
TspDTI ATGAA 1 cut(s) 17
VpaK11BI GGWCC 4 cut(s) 7, 85, 191, 239
XspI CTAG 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.