MD07G1304900.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0010599
Physical Location & Seq
Forward (+)
36207232 .. 36210508
3277 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1304900.v1.1.491

Sequence Viewer

Length: 537 bp
ATGATCCAGAAGACTATTGAGAATATGAAGTGCAGAGGGCATATGAAAAGTGGGATAATTTATGCTGTTTCGGTTGATATTTATGGGCAGTATGGAAAATTTCAGGATGCTCTAGAGTGCATATCTGCCCTAAAGGCGGAAGGTCTTATTCCTTCAGCCAGCATGTTTTGTGTCTTAGCAAATGCCTACGCTCAGCAGGGATTATGTCATCAGACAGTTAAGGTGCTTCAGCTCATGGAAGCTGAGGGAATCGAACCAAACGTCATAATGCTGAATGTGCTGATCAATGCATTCAATGTTGCTGGTAGATATTTAGAGGCGTTATCCATTTATCACCATATAAAAGAAAGTGGTTTTAGCCCTGATGTGGTTACTTATACTACCCTTATGAAGGCGTTTATTAGGGCAAGGAAGTTTGATAAGGTCCCGGAGATATACAAGGAAATAGAACGGGCCAGATGCACTCCGGATAGAAGGGCTAGACAGATGTTACAAGTTGCTTTAATGGTCCTTCAACAGAGATATTGCCTTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

179

Amino Acids

20.22

Weight (kDa)

8.88

Isoelectric Point (pI)

43.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_24 PF23276 29 - 148 6.9e-08 Fungal tetratrico peptide repeats
PPR_3 PF13812 44 - 98 3.3e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 86 - 134 8.5e-12 PPR repeat family
PPR_3 PF13812 109 - 157 4.3e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 121 - 158 1.1e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 466
AciI CCGC 1 cut(s) 137
AcsI RAATTY 1 cut(s) 98
AcuI CTGAAG 2 cut(s) 138, 212
AfiI CCNNNNNNNGG 3 cut(s) 136, 367, 391
AgsI TTSAA 2 cut(s) 295, 515
AluBI AGCT 2 cut(s) 232, 242
AluI AGCT 2 cut(s) 232, 242
Aor13HI TCCGGA 1 cut(s) 466
AoxI GGCC 1 cut(s) 453
ApoI RAATTY 1 cut(s) 98
ArsI GACNNNNNNTTYG 2 cut(s) 246, 278
AspS9I GGNCC 3 cut(s) 424, 453, 508
AsuC2I CCSGG 1 cut(s) 428
AsuHPI GGTGA 1 cut(s) 326
AvaII GGWCC 2 cut(s) 424, 508
BbsI GAAGAC 1 cut(s) 17
BbvCI CCTCAGC 1 cut(s) 243
BclI TGATCA 1 cut(s) 282
BcnI CCSGG 1 cut(s) 428
BfaI CTAG 2 cut(s) 113, 480
BglI GCCNNNNNGGC 1 cut(s) 134
BlpI GCTNAGC 1 cut(s) 192
Bme1390I CCNGG 1 cut(s) 428
Bme18I GGWCC 2 cut(s) 424, 508
BmgT120I GGNCC 3 cut(s) 424, 453, 508
BmiI GGNNCC 1 cut(s) 426
BmrFI CCNGG 1 cut(s) 428
BmsI GCATC 2 cut(s) 97, 449
BpiI GAAGAC 1 cut(s) 17
Bpu10I CCTNAGC 1 cut(s) 243
Bpu1102I GCTNAGC 1 cut(s) 192
BpuMI CCSGG 1 cut(s) 428
BsaWI WCCGGW 1 cut(s) 466
Bsc4I CCNNNNNNNGG 3 cut(s) 136, 367, 391
BseAI TCCGGA 1 cut(s) 466
BseGI GGATG 1 cut(s) 112
BseLI CCNNNNNNNGG 3 cut(s) 136, 367, 391
BseMII CTCAG 2 cut(s) 206, 234
BsgI GTGCAG 1 cut(s) 52
BshFI GGCC 1 cut(s) 455
BsiSI CCGG 2 cut(s) 428, 467
BslFI GGGAC 1 cut(s) 410
BslI CCNNNNNNNGG 3 cut(s) 136, 367, 391
BsmFI GGGAC 1 cut(s) 410
BsmI GAATGC 1 cut(s) 290
BsnI GGCC 1 cut(s) 455
Bsp13I TCCGGA 1 cut(s) 466
Bsp143I GATC 2 cut(s) 3, 282
Bsp1720I GCTNAGC 1 cut(s) 192
BspACI CCGC 1 cut(s) 137
BspANI GGCC 1 cut(s) 455
BspCNI CTCAG 2 cut(s) 205, 235
BspEI TCCGGA 1 cut(s) 466
BspLI GGNNCC 1 cut(s) 426
BssMI GATC 2 cut(s) 3, 282
Bst4CI ACNGT 1 cut(s) 217
BstC8I GCNNGC 1 cut(s) 160
BstDEI CTNAG 3 cut(s) 175, 192, 243
BstENI CCTNNNNNAGG 1 cut(s) 389
BstF5I GGATG 1 cut(s) 112
BstKTI GATC 2 cut(s) 6, 285
BstMBI GATC 2 cut(s) 3, 282
BstMWI GCNNNNNNNGC 2 cut(s) 134, 277
BstNSI RCATGY 1 cut(s) 166
BstSCI CCNGG 1 cut(s) 426
BstV2I GAAGAC 1 cut(s) 17
BsuRI GGCC 1 cut(s) 455
BtsCI GGATG 1 cut(s) 112
Cac8I GCNNGC 1 cut(s) 160
Cfr13I GGNCC 3 cut(s) 424, 453, 508
CviAII CATG 2 cut(s) 163, 235
CviJI RGCY 6 cut(s) 158, 232, 242, 360, 455, 479
CviKI_1 RGCY 6 cut(s) 158, 232, 242, 360, 455, 479
DdeI CTNAG 3 cut(s) 175, 192, 243
DpnI GATC 2 cut(s) 5, 284
DpnII GATC 2 cut(s) 3, 282
EciI GGCGGA 1 cut(s) 152
Eco47I GGWCC 2 cut(s) 424, 508
Eco57I CTGAAG 2 cut(s) 138, 212
EcoNI CCTNNNNNAGG 1 cut(s) 389
EcoO109I RGGNCCY 1 cut(s) 424
EcoT22I ATGCAT 1 cut(s) 292
FaeI CATG 2 cut(s) 166, 238
FaqI GGGAC 1 cut(s) 410
FatI CATG 2 cut(s) 162, 234
FauNDI CATATG 1 cut(s) 42
FbaI TGATCA 1 cut(s) 282
FokI GGATG 1 cut(s) 119
FspBI CTAG 2 cut(s) 113, 480
HaeIII GGCC 1 cut(s) 455
HapII CCGG 2 cut(s) 428, 467
Hin1II CATG 2 cut(s) 166, 238
HinfI GANTC 1 cut(s) 249
HpaII CCGG 2 cut(s) 428, 467
HphI GGTGA 1 cut(s) 326
Hpy188I TCNGA 1 cut(s) 213
Hpy188III TCNNGA 4 cut(s) 7, 104, 113, 467
HpyAV CCTTC 5 cut(s) 134, 162, 385, 468, 521
HpyCH4III ACNGT 1 cut(s) 217
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 4 cut(s) 33, 120, 290, 462
HpyF10VI GCNNNNNNNGC 2 cut(s) 134, 277
HpyF3I CTNAG 3 cut(s) 175, 192, 243
HpySE526I ACGT 1 cut(s) 261
Hsp92II CATG 2 cut(s) 166, 238
Kpn2I TCCGGA 1 cut(s) 466
Ksp22I TGATCA 1 cut(s) 282
Kzo9I GATC 2 cut(s) 3, 282
LpnPI CCDG 9 cut(s) 20, 89, 172, 182, 288, 375, 441, 469, 480
LweI GCATC 2 cut(s) 97, 449
MaeI CTAG 2 cut(s) 113, 480
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 2 cut(s) 370, 489
MalI GATC 2 cut(s) 5, 284
MboI GATC 2 cut(s) 3, 282
MboII GAAGA 1 cut(s) 22
MluCI AATT 2 cut(s) 57, 98
MnlI CCTC 3 cut(s) 29, 238, 310
Mph1103I ATGCAT 1 cut(s) 292
MroI TCCGGA 1 cut(s) 466
MseI TTAA 2 cut(s) 219, 503
MspI CCGG 2 cut(s) 428, 467
MspR9I CCNGG 1 cut(s) 428
Mva1269I GAATGC 1 cut(s) 290
MwoI GCNNNNNNNGC 2 cut(s) 134, 277
NciI CCSGG 1 cut(s) 428
NdeI CATATG 1 cut(s) 42
NdeII GATC 2 cut(s) 3, 282
NlaIII CATG 2 cut(s) 166, 238
NlaIV GGNNCC 1 cut(s) 426
NsiI ATGCAT 1 cut(s) 292
NspI RCATGY 1 cut(s) 166
PcsI WCGNNNNNNNCGW 1 cut(s) 258
PctI GAATGC 1 cut(s) 290
PfeI GAWTC 1 cut(s) 249
PfoI TCCNGGA 1 cut(s) 426
PpuMI RGGWCCY 1 cut(s) 424
Psp5II RGGWCCY 1 cut(s) 424
PspN4I GGNNCC 1 cut(s) 426
PspPI GGNCC 3 cut(s) 424, 453, 508
PspPPI RGGWCCY 1 cut(s) 424
SaqAI TTAA 2 cut(s) 219, 503
Sau3AI GATC 2 cut(s) 3, 282
Sau96I GGNCC 3 cut(s) 424, 453, 508
ScrFI CCNGG 1 cut(s) 428
SetI ASST 6 cut(s) 145, 225, 234, 244, 264, 426
SfaNI GCATC 2 cut(s) 97, 449
SinI GGWCC 2 cut(s) 424, 508
Sse9I AATT 2 cut(s) 57, 98
SsiI CCGC 1 cut(s) 137
SspMI CTAG 2 cut(s) 113, 480
StyD4I CCNGG 1 cut(s) 426
TaaI ACNGT 1 cut(s) 217
TaiI ACGT 1 cut(s) 264
TaqI TCGA 1 cut(s) 252
TasI AATT 2 cut(s) 57, 98
TfiI GAWTC 1 cut(s) 249
Tru1I TTAA 2 cut(s) 219, 503
Tru9I TTAA 2 cut(s) 219, 503
TspDTI ATGAA 3 cut(s) 41, 59, 404
VpaK11BI GGWCC 2 cut(s) 424, 508
XagI CCTNNNNNAGG 1 cut(s) 389
XapI RAATTY 1 cut(s) 98
XbaI TCTAGA 1 cut(s) 112
XceI RCATGY 1 cut(s) 166
XspI CTAG 2 cut(s) 113, 480
Zsp2I ATGCAT 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.