Rmu_sc0010675.1_g000007

Protein of unknown function, DUF547

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0010675.1
OG0010599
Physical Location & Seq
Forward (+)
35471 .. 41325
5855 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0010675.1_g000007.1.cds

Sequence Viewer

Length: 762 bp
atggtgggttttatctgtaacacagttggagagaaagatgggtgtcaagaacaagcaggagggaaaagagagaatggagattctgggccacgctcagatgttcatacccaacacaggcgctccaaaagtgcttctgacaagaattttggtattcaaagaggtcgagttttaagttccacaaagaaggacaagaatgaaacacaggtttcatcaccttcaacaagtgcgtctacgggacaaagtccttggcatgaaaatcctatttattttaacaagaatatgtcattgaacaacagagcatccttggaaaaagatatcgaacagctgcagttgcgtttgcaggaagagaaatctatgcgtaacatgcttgagagggcaatgggccgagcttcaagtactctatcacctggacatagacatttctctgtccagttgaaagtctacacagcctcaaatgttagagatgagcttgaagaagcaaagaaagatttcatcaaagcaaatgtagtggtaaagaagccgaggaaagtttttctaccaaagatgcttgaaagattttcgagggaagcgtcttttggctctgatgatcttctcaaatgggtcactgacaatgttgatgagaagcttcaggattcaattagaaaatgcattgatcataaatccagcaagaaggcatctcaaatcatagagtggttgccttacaattcaaggttccgatacgtgttcccaaaggatctggcagagaagccatggtggttgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF547 Lzipper-MIP1

Protein Analysis

253

Amino Acids

29.02

Weight (kDa)

9.6

Isoelectric Point (pI)

43.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 230, 441
AclWI GGATC 1 cut(s) 741
AcsI RAATTY 1 cut(s) 142
AcuI CTGAAG 1 cut(s) 611
AfaI GTAC 1 cut(s) 397
AfiI CCNNNNNNNGG 1 cut(s) 114
AflIII ACRYGT 1 cut(s) 720
AgsI TTSAA 9 cut(s) 155, 219, 289, 393, 436, 473, 551, 636, 708
AjnI CCWGG 1 cut(s) 406
AjuI GAANNNNNNNTTGG 2 cut(s) 558, 590
AluBI AGCT 4 cut(s) 325, 389, 469, 625
AluI AGCT 4 cut(s) 325, 389, 469, 625
AlwI GGATC 1 cut(s) 741
AoxI GGCC 2 cut(s) 86, 382
ApeKI GCWGC 1 cut(s) 325
ApoI RAATTY 1 cut(s) 142
ArsI GACNNNNNNTTYG 2 cut(s) 128, 160
Asp700I GAANNNNTTC 1 cut(s) 488
AspLEI GCGC 1 cut(s) 120
AspS9I GGNCC 2 cut(s) 86, 382
AsuHPI GGTGA 2 cut(s) 204, 396
BbvI GCAGC 1 cut(s) 312
BccI CCATC 1 cut(s) 32
BciT130I CCWGG 1 cut(s) 408
BclI TGATCA 1 cut(s) 652
BfmI CTRYAG 1 cut(s) 326
BfoI RGCGCY 1 cut(s) 121
BisI GCNGC 1 cut(s) 326
BlsI GCNGC 1 cut(s) 327
BmcAI AGTACT 1 cut(s) 397
Bme1390I CCNGG 1 cut(s) 408
BmgT120I GGNCC 2 cut(s) 86, 382
BmiI GGNNCC 1 cut(s) 713
BmrFI CCNGG 1 cut(s) 408
BmsI GCATC 3 cut(s) 308, 534, 683
BpuEI CTTGAG 1 cut(s) 389
BsaAI YACGTR 1 cut(s) 721
BsaJI CCNNGG 4 cut(s) 245, 303, 521, 749
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 1 cut(s) 114
Bse1I ACTGG 1 cut(s) 430
Bse3DI GCAATG 1 cut(s) 384
BseBI CCWGG 1 cut(s) 408
BseDI CCNNGG 4 cut(s) 245, 303, 521, 749
BseGI GGATG 1 cut(s) 299
BseLI CCNNNNNNNGG 1 cut(s) 114
BseMI GCAATG 1 cut(s) 384
BseMII CTCAG 1 cut(s) 108
BseNI ACTGG 1 cut(s) 430
BseXI GCAGC 1 cut(s) 312
BshFI GGCC 2 cut(s) 88, 384
BslFI GGGAC 1 cut(s) 249
BslI CCNNNNNNNGG 1 cut(s) 114
BsmFI GGGAC 1 cut(s) 249
BsnI GGCC 2 cut(s) 88, 384
Bsp143I GATC 3 cut(s) 586, 652, 733
Bsp19I CCATGG 1 cut(s) 749
BspANI GGCC 2 cut(s) 88, 384
BspCNI CTCAG 1 cut(s) 107
BspLI GGNNCC 1 cut(s) 713
BspMAI CTGCAG 1 cut(s) 330
BspPI GGATC 1 cut(s) 741
BsrDI GCAATG 1 cut(s) 384
BsrI ACTGG 1 cut(s) 430
BssECI CCNNGG 4 cut(s) 245, 303, 521, 749
BssMI GATC 3 cut(s) 586, 652, 733
BssT1I CCWWGG 3 cut(s) 245, 303, 749
Bst2UI CCWGG 1 cut(s) 408
Bst4CI ACNGT 1 cut(s) 25
Bst6I CTCTTC 1 cut(s) 339
BstBAI YACGTR 1 cut(s) 721
BstDEI CTNAG 1 cut(s) 94
BstDSI CCRYGG 1 cut(s) 749
BstF5I GGATG 1 cut(s) 299
BstH2I RGCGCY 1 cut(s) 121
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 3 cut(s) 589, 655, 736
BstMBI GATC 3 cut(s) 586, 652, 733
BstMWI GCNNNNNNNGC 2 cut(s) 331, 364
BstNI CCWGG 1 cut(s) 408
BstNSI RCATGY 1 cut(s) 367
BstSCI CCNGG 1 cut(s) 406
BstSFI CTRYAG 1 cut(s) 326
BstV1I GCAGC 1 cut(s) 312
BstX2I RGATCY 1 cut(s) 733
BstYI RGATCY 1 cut(s) 733
BsuRI GGCC 2 cut(s) 88, 384
BtgI CCRYGG 1 cut(s) 749
BtsCI GGATG 1 cut(s) 299
BtsIMutI CAGTG 1 cut(s) 603
CfoI GCGC 1 cut(s) 120
Cfr13I GGNCC 2 cut(s) 86, 382
CseI GACGC 2 cut(s) 216, 558
Csp6I GTAC 1 cut(s) 396
CspCI CAANNNNNGTGG 2 cut(s) 489, 524
CviAII CATG 3 cut(s) 251, 364, 750
CviQI GTAC 1 cut(s) 396
DdeI CTNAG 1 cut(s) 94
DpnI GATC 3 cut(s) 588, 654, 735
DpnII GATC 3 cut(s) 586, 652, 733
Eam1104I CTCTTC 1 cut(s) 339
EarI CTCTTC 1 cut(s) 339
Eco130I CCWWGG 3 cut(s) 245, 303, 749
Eco32I GATATC 1 cut(s) 316
Eco57I CTGAAG 1 cut(s) 611
EcoRII CCWGG 1 cut(s) 406
EcoRV GATATC 1 cut(s) 316
EcoT14I CCWWGG 3 cut(s) 245, 303, 749
EcoT22I ATGCAT 1 cut(s) 650
ErhI CCWWGG 3 cut(s) 245, 303, 749
FaeI CATG 3 cut(s) 254, 367, 753
FaiI YATR 9 cut(s) 105, 252, 281, 356, 365, 414, 657, 686, 751
FaqI GGGAC 1 cut(s) 249
FatI CATG 3 cut(s) 250, 363, 749
FbaI TGATCA 1 cut(s) 652
FblI GTMKAC 2 cut(s) 230, 441
Fnu4HI GCNGC 1 cut(s) 326
FokI GGATG 1 cut(s) 286
Fsp4HI GCNGC 1 cut(s) 326
GlaI GCGC 1 cut(s) 119
GluI GCNGC 1 cut(s) 326
HaeII RGCGCY 1 cut(s) 121
HaeIII GGCC 2 cut(s) 88, 384
HgaI GACGC 2 cut(s) 216, 558
HhaI GCGC 1 cut(s) 120
Hin1II CATG 3 cut(s) 254, 367, 753
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HindIII AAGCTT 1 cut(s) 623
HinfI GANTC 2 cut(s) 80, 632
HphI GGTGA 2 cut(s) 204, 396
Hpy166II GTNNAC 2 cut(s) 231, 442
Hpy188I TCNGA 4 cut(s) 97, 136, 583, 716
Hpy188III TCNNGA 2 cut(s) 47, 629
Hpy8I GTNNAC 2 cut(s) 231, 442
HpyAV CCTTC 3 cut(s) 178, 225, 664
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4IV ACGT 1 cut(s) 720
HpyCH4V TGCA 3 cut(s) 328, 340, 648
HpyF10VI GCNNNNNNNGC 2 cut(s) 331, 364
HpyF3I CTNAG 1 cut(s) 94
HpySE526I ACGT 1 cut(s) 720
Hsp92II CATG 3 cut(s) 254, 367, 753
HspAI GCGC 1 cut(s) 118
Ksp22I TGATCA 1 cut(s) 652
Kzo9I GATC 3 cut(s) 586, 652, 733
LmnI GCTCC 1 cut(s) 125
Lsp1109I GCAGC 1 cut(s) 312
LweI GCATC 3 cut(s) 308, 534, 683
MaeII ACGT 1 cut(s) 720
MaeIII GTNAC 3 cut(s) 17, 359, 601
MalI GATC 3 cut(s) 588, 654, 735
MboI GATC 3 cut(s) 586, 652, 733
MboII GAAGA 3 cut(s) 356, 485, 581
MflI RGATCY 1 cut(s) 733
MluCI AATT 3 cut(s) 142, 636, 703
MmeI TCCRAC 1 cut(s) 7
MnlI CCTC 6 cut(s) 53, 152, 366, 460, 516, 555
Mph1103I ATGCAT 1 cut(s) 650
MroXI GAANNNNTTC 1 cut(s) 488
MseI TTAA 2 cut(s) 170, 270
MspA1I CMGCKG 1 cut(s) 325
MspR9I CCNGG 1 cut(s) 408
MvaI CCWGG 1 cut(s) 408
MwoI GCNNNNNNNGC 2 cut(s) 331, 364
NcoI CCATGG 1 cut(s) 749
NdeII GATC 3 cut(s) 586, 652, 733
NlaIII CATG 3 cut(s) 254, 367, 753
NlaIV GGNNCC 1 cut(s) 713
NmeAIII GCCGAG 2 cut(s) 410, 546
NmuCI GTSAC 1 cut(s) 601
NsiI ATGCAT 1 cut(s) 650
NspI RCATGY 1 cut(s) 367
PcsI WCGNNNNNNNCGW 1 cut(s) 566
PdmI GAANNNNTTC 1 cut(s) 488
PfeI GAWTC 2 cut(s) 80, 632
PflFI GACNNNGTC 1 cut(s) 240
PkrI GCNGC 1 cut(s) 327
Ppu21I YACGTR 1 cut(s) 721
Psp6I CCWGG 1 cut(s) 406
PspGI CCWGG 1 cut(s) 406
PspN4I GGNNCC 1 cut(s) 713
PspPI GGNCC 2 cut(s) 86, 382
PstI CTGCAG 1 cut(s) 330
PsuI RGATCY 1 cut(s) 733
PsyI GACNNNGTC 1 cut(s) 240
PvuII CAGCTG 1 cut(s) 325
RsaI GTAC 1 cut(s) 397
RsaNI GTAC 1 cut(s) 396
SaqAI TTAA 2 cut(s) 170, 270
SatI GCNGC 1 cut(s) 326
Sau3AI GATC 3 cut(s) 586, 652, 733
Sau96I GGNCC 2 cut(s) 86, 382
ScaI AGTACT 1 cut(s) 397
ScrFI CCNGG 1 cut(s) 408
SfaNI GCATC 3 cut(s) 308, 534, 683
SfcI CTRYAG 1 cut(s) 326
SmlI CTYRAG 1 cut(s) 368
SmoI CTYRAG 1 cut(s) 368
Sse9I AATT 3 cut(s) 142, 636, 703
StyD4I CCNGG 1 cut(s) 406
StyI CCWWGG 3 cut(s) 245, 303, 749
TaaI ACNGT 1 cut(s) 25
TaiI ACGT 1 cut(s) 723
TaqI TCGA 3 cut(s) 163, 318, 560
TasI AATT 3 cut(s) 142, 636, 703
TatI WGTACW 1 cut(s) 395
TfiI GAWTC 2 cut(s) 80, 632
Tru1I TTAA 2 cut(s) 170, 270
Tru9I TTAA 2 cut(s) 170, 270
TscAI CASTG 1 cut(s) 610
TseFI GTSAC 1 cut(s) 601
TseI GCWGC 1 cut(s) 325
Tsp45I GTSAC 1 cut(s) 601
TspDTI ATGAA 5 cut(s) 92, 198, 210, 267, 481
TspRI CASTG 1 cut(s) 610
Tth111I GACNNNGTC 1 cut(s) 240
XapI RAATTY 1 cut(s) 142
XceI RCATGY 1 cut(s) 367
XmiI GTMKAC 2 cut(s) 230, 441
XmnI GAANNNNTTC 1 cut(s) 488
ZrmI AGTACT 1 cut(s) 397
Zsp2I ATGCAT 1 cut(s) 650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.