Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0010820

Physical Location & Seq

Genomic Coordinates Forward (+)

3414619 .. 3416170

Gene Structure 1552 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1045000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 894 bp

ATGGAACTCCACAAGGACGTTTCCAAGAAGCAGAAGTTCGAGGTCGCCGTCACCGCTCTTAATTCGCTGCTCCGGGACCACTACTCCTCCGCTTCTCCGTCGCTGCGGAAACTGTACTACGGCGTCGTTTGCCGGGTTGCCACCGTGTTGAAGACCCGGTACACCTCCCCTGGTTTCTGGGCCGCCGGACTCGCTCTCTTTCAGCTCGCCCATTCCCTCGTTTCAGACCCTTCCGAGAAGGCCCACTTGCTCGCTTGCATCGATGAGGCACGACAGGTCGTTCATCAGGAAAACGACCCCGCGCAGCCGTCGTCTTCTCCGAATCAAGGATATTTGTTTGAGGGGCATTTGACGGTGGATCGGGAGCCGCCGCAGCCGCAGTGGCTGTTGCAGTCGAATCTATTGACGGCGGCCATGGCGGTGGGGTCGTCTTCTGAGTCAGGTCCCGGAATCGAAGAGAACTCGGAAGGCGCAGTGAATCTGCTTCAGTCGCTCATTGACAACCTCGACAGCGTTTTGCCGCCGGGGATTTTGGATGACGTGAGGGCGGCTCCGAGGGGCCCGCCTGCGAGCAAACAGGTGGTGGCCAATCTTCCGGTGATCATGATCACGGAGGAAGTGTTGAAGGAGCTCGGAGAGGACGCAGATTGCTGCATTTGTAAGGAAAACTTGGTTGTCAATGACAAAATGCAGGAGTTGCCCTGCAAGCACACTTTTCATCCTCCTTGCCTAAAGCCATGGCTGGATGAGCACAATTCTTGTCCGATATGCCGGCATGAGCTGCAGACGGATGATCATGTGTACGAGAACTGGAAGGAGAGGGAGAAGGAGGCCGAAGAAGACAGAAAAGGAGCTGCAAATGCGATTCGAGGTGGTGAATACATGTATGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009737 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010498 GO:0010646 GO:0010648 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0023051 GO:0023057 GO:0030163 GO:0032446 GO:0033993 GO:0036211 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0051603 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0097305 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1905957 GO:1905958

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

298

Amino Acids

32.9

Weight (kDa)

5.16

Isoelectric Point (pI)

53.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	216 - 258	1.1e-12	Ring finger domain
zf-RING_11	PF17123	216 - 244	4.4e-07	RING-like zinc finger
zf-C3HC4_2	PF13923	217 - 257	1.1e-06	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010820)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20910
fragaria_vesca	FvH4_2g37060
malus_domestica	MD08G1042800.v1.1 MD08G1045000.v1.1 MD08G1045700.v1.1 MD15G1046800.v1.1
prunus_persica	Prupe.1G401200_v2.0.a1 Prupe.1G401200_v2.0.a1
pyrus_communis	pycom15g04480
rosa_chinensis	RchiOBHm_Chr6g0304721
rosa_laevigata	RLG00000010956
rosa_multiflora	Rmu_co8160404.1_g000001 Rmu_sc0027256.1_g000001
rosa_roxburghii	Rroxscaffold_7G00163560
rosa_rugosa	Rorug06G0333800
rosa_samantha	Rh6AG445800 Rh6BG455900 Rh6CG458900 Rh6DG445900
rosa_wichuraiana	Rw6G038780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	56
AccII	CGCG	1 cut(s)	302
AclWI	GGATC	1 cut(s)	366
AcoI	YGGCCR	2 cut(s)	411, 585
AcuI	CTGAAG	1 cut(s)	470
AcyI	GRCGYC	1 cut(s)	123
AfaI	GTAC	3 cut(s)	116, 161, 803
AfiI	CCNNNNNNNGG	1 cut(s)	326
AflIII	ACRYGT	1 cut(s)	882
AgsI	TTSAA	2 cut(s)	151, 625
AjiI	CACGTC	1 cut(s)	541
AjnI	CCWGG	1 cut(s)	169
AluBI	AGCT	4 cut(s)	205, 631, 781, 854
AluI	AGCT	4 cut(s)	205, 631, 781, 854
Alw21I	GWGCWC	2 cut(s)	633, 753
AlwI	GGATC	1 cut(s)	366
AlwNI	CAGNNNCTG	1 cut(s)	385
AoxI	GGCC	6 cut(s)	180, 240, 411, 559, 585, 831
ApaI	GGGCCC	1 cut(s)	563
ApeKI	GCWGC	7 cut(s)	67, 103, 304, 373, 651, 781, 854
AspLEI	GCGC	2 cut(s)	304, 473
AspS9I	GGNCC	6 cut(s)	76, 180, 241, 443, 559, 560
AsuC2I	CCSGG	5 cut(s)	74, 134, 157, 447, 525
AsuHPI	GGTGA	3 cut(s)	43, 610, 887
AvaII	GGWCC	2 cut(s)	76, 443
BaeGI	GKGCMC	1 cut(s)	563
BalI	TGGCCA	1 cut(s)	587
BanII	GRGCYC	2 cut(s)	563, 633
BarI	GAAGNNNNNNTAC	2 cut(s)	143, 175
BbsI	GAAGAC	4 cut(s)	158, 306, 423, 846
Bbv12I	GWGCWC	2 cut(s)	633, 753
BbvI	GCAGC	7 cut(s)	54, 90, 316, 385, 638, 768, 841
BceAI	ACGGC	4 cut(s)	32, 136, 292, 423
BciT130I	CCWGG	1 cut(s)	171
BclI	TGATCA	3 cut(s)	600, 606, 793
BcnI	CCSGG	5 cut(s)	74, 134, 157, 447, 525
BfmI	CTRYAG	1 cut(s)	782
BglI	GCCNNNNNGGC	1 cut(s)	382
Bme1390I	CCNGG	6 cut(s)	74, 134, 157, 171, 447, 525
Bme18I	GGWCC	2 cut(s)	76, 443
BmgBI	CACGTC	1 cut(s)	541
BmgT120I	GGNCC	6 cut(s)	76, 180, 241, 443, 559, 560
BmiI	GGNNCC	6 cut(s)	77, 366, 445, 552, 560, 561
BmrFI	CCNGG	6 cut(s)	74, 134, 157, 171, 447, 525
BmsI	GCATC	1 cut(s)	267
BpiI	GAAGAC	4 cut(s)	158, 306, 423, 846
BplI	GAGNNNNNCTC	2 cut(s)	535, 567
BpuMI	CCSGG	5 cut(s)	74, 134, 157, 447, 525
Bsa29I	ATCGAT	1 cut(s)	261
BsaBI	GATNNNNATC	1 cut(s)	605
BsaHI	GRCGYC	1 cut(s)	123
BsaJI	CCNNGG	5 cut(s)	169, 414, 524, 554, 737
BsaWI	WCCGGW	1 cut(s)	595
BsaXI	ACNNNNNCTCC	6 cut(s)	68, 71, 98, 101, 605, 635
Bsc4I	CCNNNNNNNGG	1 cut(s)	326
Bse118I	RCCGGY	1 cut(s)	771
Bse1I	ACTGG	1 cut(s)	815
Bse8I	GATNNNNATC	1 cut(s)	605
BseBI	CCWGG	1 cut(s)	171
BseCI	ATCGAT	1 cut(s)	261
BseDI	CCNNGG	5 cut(s)	169, 414, 524, 554, 737
BseGI	GGATG	4 cut(s)	541, 718, 751, 796
BseJI	GATNNNNATC	1 cut(s)	605
BseLI	CCNNNNNNNGG	1 cut(s)	326
BseMII	CTCAG	1 cut(s)	426
BseNI	ACTGG	1 cut(s)	815
BseRI	GAGGAG	1 cut(s)	76
BseSI	GKGCMC	1 cut(s)	563
BseXI	GCAGC	7 cut(s)	54, 90, 316, 385, 638, 768, 841
Bsh1236I	CGCG	1 cut(s)	302
BshFI	GGCC	6 cut(s)	182, 242, 413, 561, 587, 833
BshVI	ATCGAT	1 cut(s)	261
BsiHKAI	GWGCWC	2 cut(s)	633, 753
BsiSI	CCGG	8 cut(s)	73, 133, 157, 186, 447, 524, 596, 772
BslFI	GGGAC	2 cut(s)	89, 429
BslI	CCNNNNNNNGG	1 cut(s)	326
BsmFI	GGGAC	2 cut(s)	89, 429
BsnI	GGCC	6 cut(s)	182, 242, 413, 561, 587, 833
Bsp120I	GGGCCC	1 cut(s)	559
Bsp1286I	GDGCHC	3 cut(s)	563, 633, 753
Bsp143I	GATC	4 cut(s)	358, 600, 606, 793
Bsp19I	CCATGG	2 cut(s)	414, 737
BspANI	GGCC	6 cut(s)	182, 242, 413, 561, 587, 833
BspCNI	CTCAG	1 cut(s)	427
BspDI	ATCGAT	1 cut(s)	261
BspFNI	CGCG	1 cut(s)	302
BspHI	TCATGA	1 cut(s)	603
BspLI	GGNNCC	6 cut(s)	77, 366, 445, 552, 560, 561
BspMAI	CTGCAG	1 cut(s)	786
BspPI	GGATC	1 cut(s)	366
BsrBI	CCGCTC	1 cut(s)	56
BsrFI	RCCGGY	1 cut(s)	771
BsrI	ACTGG	1 cut(s)	815
BssAI	RCCGGY	1 cut(s)	771
BssECI	CCNNGG	5 cut(s)	169, 414, 524, 554, 737
BssMI	GATC	4 cut(s)	358, 600, 606, 793
BssNI	GRCGYC	1 cut(s)	123
BssT1I	CCWWGG	2 cut(s)	414, 737
Bst2UI	CCWGG	1 cut(s)	171
Bst4CI	ACNGT	3 cut(s)	114, 145, 355
Bst6I	CTCTTC	1 cut(s)	450
BstACI	GRCGYC	1 cut(s)	123
BstAPI	GCANNNNNTGC	2 cut(s)	697, 781
BstC8I	GCNNGC	8 cut(s)	207, 252, 256, 563, 567, 571, 707, 773
BstDEI	CTNAG	1 cut(s)	435
BstDSI	CCRYGG	2 cut(s)	414, 737
BstF5I	GGATG	4 cut(s)	541, 718, 751, 796
BstFNI	CGCG	1 cut(s)	302
BstHHI	GCGC	2 cut(s)	304, 473
BstKTI	GATC	4 cut(s)	361, 603, 609, 796
BstMBI	GATC	4 cut(s)	358, 600, 606, 793
BstNI	CCWGG	1 cut(s)	171
BstNSI	RCATGY	1 cut(s)	886
BstSCI	CCNGG	6 cut(s)	72, 132, 155, 169, 445, 523
BstSFI	CTRYAG	1 cut(s)	782
BstSLI	GKGCMC	1 cut(s)	563
BstUI	CGCG	1 cut(s)	302
BstV1I	GCAGC	7 cut(s)	54, 90, 316, 385, 638, 768, 841
BstV2I	GAAGAC	4 cut(s)	158, 306, 423, 846
BstXI	CCANNNNNNTGG	1 cut(s)	421
Bsu15I	ATCGAT	1 cut(s)	261
BsuRI	GGCC	6 cut(s)	182, 242, 413, 561, 587, 833
BsuTUI	ATCGAT	1 cut(s)	261
BtgI	CCRYGG	2 cut(s)	414, 737
BtrI	CACGTC	1 cut(s)	541
BtsCI	GGATG	4 cut(s)	541, 718, 751, 796
BtsI	GCAGTG	2 cut(s)	386, 480
BtsIMutI	CAGTG	2 cut(s)	386, 480
Cac8I	GCNNGC	8 cut(s)	207, 252, 256, 563, 567, 571, 707, 773
CaiI	CAGNNNCTG	1 cut(s)	385
CciI	TCATGA	1 cut(s)	603
CfoI	GCGC	2 cut(s)	304, 473
Cfr10I	RCCGGY	1 cut(s)	771
Cfr13I	GGNCC	6 cut(s)	76, 180, 241, 443, 559, 560
ClaI	ATCGAT	1 cut(s)	261
CseI	GACGC	2 cut(s)	112, 650
Csp6I	GTAC	3 cut(s)	115, 160, 802
CviAII	CATG	6 cut(s)	415, 604, 738, 776, 797, 883
CviQI	GTAC	3 cut(s)	115, 160, 802
DdeI	CTNAG	1 cut(s)	435
DpnI	GATC	4 cut(s)	360, 602, 608, 795
DpnII	GATC	4 cut(s)	358, 600, 606, 793
EaeI	YGGCCR	2 cut(s)	411, 585
Eam1104I	CTCTTC	1 cut(s)	450
EarI	CTCTTC	1 cut(s)	450
Ecl136II	GAGCTC	1 cut(s)	631
Eco130I	CCWWGG	2 cut(s)	414, 737
Eco24I	GRGCYC	2 cut(s)	563, 633
Eco47I	GGWCC	2 cut(s)	76, 443
Eco53kI	GAGCTC	1 cut(s)	631
Eco57I	CTGAAG	1 cut(s)	470
EcoICRI	GAGCTC	1 cut(s)	631
EcoO109I	RGGNCCY	2 cut(s)	443, 559
EcoRII	CCWGG	1 cut(s)	169
EcoT14I	CCWWGG	2 cut(s)	414, 737
EcoT38I	GRGCYC	2 cut(s)	563, 633
ErhI	CCWWGG	2 cut(s)	414, 737
FaeI	CATG	6 cut(s)	418, 607, 741, 779, 800, 886
FaiI	YATR	8 cut(s)	416, 605, 739, 769, 777, 798, 884, 888
FalI	AAGNNNNNCTT	4 cut(s)	230, 262, 653, 685
FaqI	GGGAC	2 cut(s)	89, 429
FatI	CATG	6 cut(s)	414, 603, 737, 775, 796, 882
FauI	CCCGC	2 cut(s)	307, 570
FbaI	TGATCA	3 cut(s)	600, 606, 793
FokI	GGATG	4 cut(s)	548, 705, 758, 803
FriOI	GRGCYC	2 cut(s)	563, 633
GlaI	GCGC	2 cut(s)	303, 472
HaeIII	GGCC	6 cut(s)	182, 242, 413, 561, 587, 833
HapII	CCGG	8 cut(s)	73, 133, 157, 186, 447, 524, 596, 772
HgaI	GACGC	2 cut(s)	112, 650
HhaI	GCGC	2 cut(s)	304, 473
Hin1I	GRCGYC	1 cut(s)	123
Hin1II	CATG	6 cut(s)	418, 607, 741, 779, 800, 886
Hin6I	GCGC	2 cut(s)	302, 471
HinP1I	GCGC	2 cut(s)	302, 471
HinfI	GANTC	7 cut(s)	189, 322, 397, 437, 450, 478, 865
HpaII	CCGG	8 cut(s)	73, 133, 157, 186, 447, 524, 596, 772
HphI	GGTGA	3 cut(s)	43, 610, 887
Hpy166II	GTNNAC	2 cut(s)	162, 802
Hpy188I	TCNGA	8 cut(s)	226, 235, 321, 436, 466, 555, 635, 765
Hpy188III	TCNNGA	3 cut(s)	287, 362, 604
Hpy8I	GTNNAC	2 cut(s)	162, 802
Hpy99I	CGWCG	3 cut(s)	103, 128, 313
HpyAV	CCTTC	6 cut(s)	232, 240, 461, 619, 808, 820
HpyCH4III	ACNGT	3 cut(s)	114, 145, 355
HpyCH4IV	ACGT	2 cut(s)	18, 540
HpyCH4V	TGCA	7 cut(s)	258, 391, 654, 691, 705, 784, 857
HpyF3I	CTNAG	1 cut(s)	435
HpySE526I	ACGT	2 cut(s)	18, 540
Hsp92I	GRCGYC	1 cut(s)	123
Hsp92II	CATG	6 cut(s)	418, 607, 741, 779, 800, 886
HspAI	GCGC	2 cut(s)	302, 471
KroI	GCCGGC	1 cut(s)	771
KroNI	GCCGGC	1 cut(s)	773
Ksp22I	TGATCA	3 cut(s)	600, 606, 793
Kzo9I	GATC	4 cut(s)	358, 600, 606, 793
LmnI	GCTCC	5 cut(s)	75, 364, 556, 628, 851
Lsp1109I	GCAGC	7 cut(s)	54, 90, 316, 385, 638, 768, 841
LweI	GCATC	1 cut(s)	267
MaeII	ACGT	2 cut(s)	18, 540
MaeIII	GTNAC	1 cut(s)	49
MalI	GATC	4 cut(s)	360, 602, 608, 795
MbiI	CCGCTC	1 cut(s)	56
MboI	GATC	4 cut(s)	358, 600, 606, 793
MboII	GAAGA	7 cut(s)	163, 306, 423, 467, 584, 848, 851
MhlI	GDGCHC	3 cut(s)	563, 633, 753
MlsI	TGGCCA	1 cut(s)	587
MluCI	AATT	2 cut(s)	61, 754
MluNI	TGGCCA	1 cut(s)	587
MlyI	GAGTC	2 cut(s)	183, 446
Mox20I	TGGCCA	1 cut(s)	587
MroNI	GCCGGC	1 cut(s)	771
MscI	TGGCCA	1 cut(s)	587
MseI	TTAA	1 cut(s)	60
MslI	CAYNNNNRTG	1 cut(s)	419
Msp20I	TGGCCA	1 cut(s)	587
MspI	CCGG	8 cut(s)	73, 133, 157, 186, 447, 524, 596, 772
MspR9I	CCNGG	6 cut(s)	74, 134, 157, 171, 447, 525
MvaI	CCWGG	1 cut(s)	171
MvnI	CGCG	1 cut(s)	302
NaeI	GCCGGC	1 cut(s)	773
NciI	CCSGG	5 cut(s)	74, 134, 157, 447, 525
NcoI	CCATGG	2 cut(s)	414, 737
NdeII	GATC	4 cut(s)	358, 600, 606, 793
NgoMIV	GCCGGC	1 cut(s)	771
NlaIII	CATG	6 cut(s)	418, 607, 741, 779, 800, 886
NlaIV	GGNNCC	6 cut(s)	77, 366, 445, 552, 560, 561
NmuCI	GTSAC	1 cut(s)	49
NspI	RCATGY	1 cut(s)	886
PagI	TCATGA	1 cut(s)	603
PciI	ACATGT	1 cut(s)	882
PcsI	WCGNNNNNNNCGW	3 cut(s)	45, 258, 317
PdiI	GCCGGC	1 cut(s)	773
PfeI	GAWTC	5 cut(s)	322, 397, 450, 478, 865
PfoI	TCCNGGA	2 cut(s)	72, 445
PleI	GAGTC	2 cut(s)	183, 445
PpsI	GAGTC	2 cut(s)	183, 445
PpuMI	RGGWCCY	1 cut(s)	443
PscI	ACATGT	1 cut(s)	882
Psp124BI	GAGCTC	1 cut(s)	633
Psp5II	RGGWCCY	1 cut(s)	443
Psp6I	CCWGG	1 cut(s)	169
PspGI	CCWGG	1 cut(s)	169
PspN4I	GGNNCC	6 cut(s)	77, 366, 445, 552, 560, 561
PspOMI	GGGCCC	1 cut(s)	559
PspPI	GGNCC	6 cut(s)	76, 180, 241, 443, 559, 560
PspPPI	RGGWCCY	1 cut(s)	443
PstI	CTGCAG	1 cut(s)	786
PstNI	CAGNNNCTG	1 cut(s)	385
RsaI	GTAC	3 cut(s)	116, 161, 803
RsaNI	GTAC	3 cut(s)	115, 160, 802
RseI	CAYNNNNRTG	1 cut(s)	419
SacI	GAGCTC	1 cut(s)	633
SaqAI	TTAA	1 cut(s)	60
Sau3AI	GATC	4 cut(s)	358, 600, 606, 793
Sau96I	GGNCC	6 cut(s)	76, 180, 241, 443, 559, 560
SchI	GAGTC	2 cut(s)	183, 446
ScrFI	CCNGG	6 cut(s)	74, 134, 157, 171, 447, 525
SduI	GDGCHC	3 cut(s)	563, 633, 753
SfaNI	GCATC	1 cut(s)	267
SfcI	CTRYAG	1 cut(s)	782
SinI	GGWCC	2 cut(s)	76, 443
SmiMI	CAYNNNNRTG	1 cut(s)	419
Sse9I	AATT	2 cut(s)	61, 754
SstI	GAGCTC	1 cut(s)	633
StyD4I	CCNGG	6 cut(s)	72, 132, 155, 169, 445, 523
StyI	CCWWGG	2 cut(s)	414, 737
TaaI	ACNGT	3 cut(s)	114, 145, 355
TaiI	ACGT	2 cut(s)	21, 543
TaqI	TCGA	6 cut(s)	39, 261, 395, 453, 507, 868
TasI	AATT	2 cut(s)	61, 754
TatI	WGTACW	1 cut(s)	114
TauI	GCSGC	7 cut(s)	185, 370, 373, 379, 413, 523, 551
TfiI	GAWTC	5 cut(s)	322, 397, 450, 478, 865
Tru1I	TTAA	1 cut(s)	60
Tru9I	TTAA	1 cut(s)	60
TscAI	CASTG	2 cut(s)	386, 480
TseFI	GTSAC	1 cut(s)	49
TseI	GCWGC	7 cut(s)	67, 103, 304, 373, 651, 781, 854
Tsp45I	GTSAC	1 cut(s)	49
TspDTI	ATGAA	2 cut(s)	272, 707
TspGWI	ACGGA	3 cut(s)	87, 626, 803
TspRI	CASTG	2 cut(s)	386, 480
VpaK11BI	GGWCC	2 cut(s)	76, 443
XceI	RCATGY	1 cut(s)	886

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.