MD08G1087300.v1.1

Ribosomal protein S14p/S29e

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011986
Physical Location & Seq
Reverse (-)
7244094 .. 7244899
806 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1087300.v1.1.491

Sequence Viewer

Length: 219 bp
ATGGGACACTCGAACGTCTGGAACTCTCACCCCAAGAACTACGGCCCGGGTTCCCGCACTTGCCGAGTGTGTGGAAACCCTCATGGGTTGATCCGGAAGTACGCGCTGATGTGCTGCAGGCAGTGCTTCCGCAGCAACGCCAAGGAGATTGGCTTCATCAAGGTTTCGATACTTCTGTATCTTCATTTTATTAGCGGACTGAGAAAATATACCATATAG

Protein Analysis

73

Amino Acids

8.24

Weight (kDa)

9.98

Isoelectric Point (pI)

23.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S14 PF00253 7 - 56 7.8e-15 Ribosomal protein S14p/S29e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 104
AccIII TCCGGA 1 cut(s) 93
AciI CCGC 3 cut(s) 55, 130, 195
AclWI GGATC 1 cut(s) 85
AfaI GTAC 1 cut(s) 101
AlwI GGATC 1 cut(s) 85
Ama87I CYCGRG 1 cut(s) 46
Aor13HI TCCGGA 1 cut(s) 93
AoxI GGCC 1 cut(s) 43
ApeKI GCWGC 2 cut(s) 114, 132
AspLEI GCGC 1 cut(s) 106
AspS9I GGNCC 1 cut(s) 44
AsuC2I CCSGG 2 cut(s) 47, 48
AsuHPI GGTGA 1 cut(s) 20
AvaI CYCGRG 1 cut(s) 46
BaeI ACNNNNGTAYC 4 cut(s) 161, 161, 194, 194
BbvI GCAGC 2 cut(s) 101, 144
BceAI ACGGC 1 cut(s) 58
BcnI CCSGG 2 cut(s) 47, 48
BfmI CTRYAG 1 cut(s) 115
BisI GCNGC 2 cut(s) 115, 133
BlsI GCNGC 2 cut(s) 116, 134
Bme1390I CCNGG 2 cut(s) 47, 48
BmeT110I CYCGRG 1 cut(s) 46
BmgT120I GGNCC 1 cut(s) 44
BmiI GGNNCC 1 cut(s) 52
BmrFI CCNGG 2 cut(s) 47, 48
BpuMI CCSGG 2 cut(s) 47, 48
BsaJI CCNNGG 2 cut(s) 46, 141
BsaWI WCCGGW 1 cut(s) 93
BseAI TCCGGA 1 cut(s) 93
BseDI CCNNGG 2 cut(s) 46, 141
BseMII CTCAG 1 cut(s) 191
BseXI GCAGC 2 cut(s) 101, 144
Bsh1236I CGCG 1 cut(s) 104
BshFI GGCC 1 cut(s) 45
BsiHKCI CYCGRG 1 cut(s) 46
BsiSI CCGG 2 cut(s) 47, 94
BslFI GGGAC 1 cut(s) 18
BsmFI GGGAC 1 cut(s) 18
BsnI GGCC 1 cut(s) 45
BsoBI CYCGRG 1 cut(s) 46
Bsp13I TCCGGA 1 cut(s) 93
Bsp143I GATC 1 cut(s) 90
BspACI CCGC 3 cut(s) 55, 130, 195
BspANI GGCC 1 cut(s) 45
BspCNI CTCAG 1 cut(s) 192
BspEI TCCGGA 1 cut(s) 93
BspFNI CGCG 1 cut(s) 104
BspLI GGNNCC 1 cut(s) 52
BspMAI CTGCAG 1 cut(s) 119
BspPI GGATC 1 cut(s) 85
BssECI CCNNGG 2 cut(s) 46, 141
BssMI GATC 1 cut(s) 90
BssT1I CCWWGG 1 cut(s) 141
BstAPI GCANNNNNTGC 1 cut(s) 123
BstC8I GCNNGC 1 cut(s) 119
BstDEI CTNAG 1 cut(s) 200
BstFNI CGCG 1 cut(s) 104
BstHHI GCGC 1 cut(s) 106
BstKTI GATC 1 cut(s) 93
BstMBI GATC 1 cut(s) 90
BstMWI GCNNNNNNNGC 2 cut(s) 123, 132
BstSCI CCNGG 2 cut(s) 45, 46
BstSFI CTRYAG 1 cut(s) 115
BstUI CGCG 1 cut(s) 104
BstV1I GCAGC 2 cut(s) 101, 144
BsuRI GGCC 1 cut(s) 45
BtsI GCAGTG 1 cut(s) 128
BtsIMutI CAGTG 1 cut(s) 128
Cac8I GCNNGC 1 cut(s) 119
CfoI GCGC 1 cut(s) 106
Cfr13I GGNCC 1 cut(s) 44
Cfr9I CCCGGG 1 cut(s) 46
Csp6I GTAC 1 cut(s) 100
CviAII CATG 1 cut(s) 83
CviJI RGCY 2 cut(s) 45, 153
CviKI_1 RGCY 2 cut(s) 45, 153
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 1 cut(s) 200
DpnI GATC 1 cut(s) 92
DpnII GATC 1 cut(s) 90
Eco130I CCWWGG 1 cut(s) 141
Eco88I CYCGRG 1 cut(s) 46
EcoT14I CCWWGG 1 cut(s) 141
ErhI CCWWGG 1 cut(s) 141
FaeI CATG 1 cut(s) 86
FaiI YATR 4 cut(s) 84, 210, 215, 217
FaqI GGGAC 1 cut(s) 18
FatI CATG 1 cut(s) 82
FauI CCCGC 1 cut(s) 62
Fnu4HI GCNGC 2 cut(s) 115, 133
Fsp4HI GCNGC 2 cut(s) 115, 133
GlaI GCGC 1 cut(s) 105
GluI GCNGC 2 cut(s) 115, 133
HaeIII GGCC 1 cut(s) 45
HapII CCGG 2 cut(s) 47, 94
HhaI GCGC 1 cut(s) 106
Hin1II CATG 1 cut(s) 86
Hin6I GCGC 1 cut(s) 104
HinP1I GCGC 1 cut(s) 104
HpaII CCGG 2 cut(s) 47, 94
HphI GGTGA 1 cut(s) 20
Hpy188III TCNNGA 2 cut(s) 19, 94
HpyCH4IV ACGT 1 cut(s) 15
HpyCH4V TGCA 1 cut(s) 117
HpyF10VI GCNNNNNNNGC 2 cut(s) 123, 132
HpyF3I CTNAG 1 cut(s) 200
HpySE526I ACGT 1 cut(s) 15
Hsp92II CATG 1 cut(s) 86
HspAI GCGC 1 cut(s) 104
Kpn2I TCCGGA 1 cut(s) 93
Kzo9I GATC 1 cut(s) 90
LpnPI CCDG 4 cut(s) 4, 60, 103, 107
Lsp1109I GCAGC 2 cut(s) 101, 144
MaeII ACGT 1 cut(s) 15
MalI GATC 1 cut(s) 92
MboI GATC 1 cut(s) 90
MboII GAAGA 1 cut(s) 173
MnlI CCTC 1 cut(s) 90
MroI TCCGGA 1 cut(s) 93
MspI CCGG 2 cut(s) 47, 94
MspR9I CCNGG 2 cut(s) 47, 48
MvnI CGCG 1 cut(s) 104
MwoI GCNNNNNNNGC 2 cut(s) 123, 132
NciI CCSGG 2 cut(s) 47, 48
NdeII GATC 1 cut(s) 90
NlaIII CATG 1 cut(s) 86
NlaIV GGNNCC 1 cut(s) 52
NmeAIII GCCGAG 1 cut(s) 89
PkrI GCNGC 2 cut(s) 116, 134
PspN4I GGNNCC 1 cut(s) 52
PspPI GGNCC 1 cut(s) 44
PstI CTGCAG 1 cut(s) 119
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
SatI GCNGC 2 cut(s) 115, 133
Sau3AI GATC 1 cut(s) 90
Sau96I GGNCC 1 cut(s) 44
ScrFI CCNGG 2 cut(s) 47, 48
SetI ASST 2 cut(s) 18, 165
SfcI CTRYAG 1 cut(s) 115
SmaI CCCGGG 1 cut(s) 48
SsiI CCGC 3 cut(s) 55, 130, 195
StyD4I CCNGG 2 cut(s) 45, 46
StyI CCWWGG 1 cut(s) 141
TaiI ACGT 1 cut(s) 18
TaqI TCGA 2 cut(s) 11, 167
TscAI CASTG 1 cut(s) 128
TseI GCWGC 2 cut(s) 114, 132
TspDTI ATGAA 2 cut(s) 145, 173
TspMI CCCGGG 1 cut(s) 46
TspRI CASTG 1 cut(s) 128
XmaI CCCGGG 1 cut(s) 46
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.