Rw6G005310

40S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0011986
Physical Location & Seq
Forward (+)
8534699 .. 8535182
484 bp
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UTR
Exon/CDS
Intron
Rw6G005310.1

Sequence Viewer

Length: 183 bp
ATGGGGCACTCCAACGTCTGGAACTCTCACCCTAAGAGCTACGGCCCTGGATCCCGCACCTGCCGTGTGTGTGGAAACCCTCACGGGTTGATCCGCAAGTACGGGATCATGTGCTGCAGGCAGTGCTTCCGCAGCAACGCCAAGGAGATTGGCTTCATTAAGGTGAGGCTTTGTCAACCTTGA

Protein Analysis

60

Amino Acids

6.78

Weight (kDa)

9.89

Isoelectric Point (pI)

18.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S14 PF00253 7 - 54 2.8e-14 Ribosomal protein S14p/S29e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 68
Acc36I ACCTGC 1 cut(s) 68
AccB7I CCANNNNNTGG 1 cut(s) 18
AciI CCGC 3 cut(s) 55, 94, 130
AclWI GGATC 4 cut(s) 45, 58, 85, 113
AfaI GTAC 1 cut(s) 101
AfiI CCNNNNNNNGG 1 cut(s) 18
AjnI CCWGG 1 cut(s) 46
AluBI AGCT 1 cut(s) 39
AluI AGCT 1 cut(s) 39
AlwI GGATC 4 cut(s) 45, 58, 85, 113
AoxI GGCC 1 cut(s) 43
ApeKI GCWGC 2 cut(s) 114, 132
AspS9I GGNCC 1 cut(s) 44
AsuHPI GGTGA 2 cut(s) 20, 175
BaeGI GKGCMC 1 cut(s) 9
BamHI GGATCC 1 cut(s) 50
BbvI GCAGC 2 cut(s) 101, 144
BceAI ACGGC 2 cut(s) 48, 58
BciT130I CCWGG 1 cut(s) 48
BfmI CTRYAG 1 cut(s) 115
BfuAI ACCTGC 1 cut(s) 68
BisI GCNGC 2 cut(s) 115, 133
BlsI GCNGC 2 cut(s) 116, 134
Bme1390I CCNGG 1 cut(s) 48
BmgT120I GGNCC 1 cut(s) 44
BmiI GGNNCC 1 cut(s) 52
BmrFI CCNGG 1 cut(s) 48
BsaJI CCNNGG 2 cut(s) 46, 141
Bsc4I CCNNNNNNNGG 1 cut(s) 18
BseBI CCWGG 1 cut(s) 48
BseDI CCNNGG 2 cut(s) 46, 141
BseLI CCNNNNNNNGG 1 cut(s) 18
BseSI GKGCMC 1 cut(s) 9
BseXI GCAGC 2 cut(s) 101, 144
BshFI GGCC 1 cut(s) 45
BslI CCNNNNNNNGG 1 cut(s) 18
BsnI GGCC 1 cut(s) 45
Bsp1286I GDGCHC 1 cut(s) 9
Bsp143I GATC 3 cut(s) 50, 90, 105
BspACI CCGC 3 cut(s) 55, 94, 130
BspANI GGCC 1 cut(s) 45
BspLI GGNNCC 1 cut(s) 52
BspMAI CTGCAG 1 cut(s) 119
BspMI ACCTGC 1 cut(s) 68
BspPI GGATC 4 cut(s) 45, 58, 85, 113
BssECI CCNNGG 2 cut(s) 46, 141
BssMI GATC 3 cut(s) 50, 90, 105
BssT1I CCWWGG 1 cut(s) 141
Bst2UI CCWGG 1 cut(s) 48
BstAPI GCANNNNNTGC 1 cut(s) 123
BstC8I GCNNGC 1 cut(s) 119
BstDEI CTNAG 1 cut(s) 33
BstKTI GATC 3 cut(s) 53, 93, 108
BstMBI GATC 3 cut(s) 50, 90, 105
BstMWI GCNNNNNNNGC 2 cut(s) 123, 132
BstNI CCWGG 1 cut(s) 48
BstSCI CCNGG 1 cut(s) 46
BstSFI CTRYAG 1 cut(s) 115
BstSLI GKGCMC 1 cut(s) 9
BstV1I GCAGC 2 cut(s) 101, 144
BstX2I RGATCY 1 cut(s) 50
BstYI RGATCY 1 cut(s) 50
BsuRI GGCC 1 cut(s) 45
BtsI GCAGTG 1 cut(s) 128
BtsIMutI CAGTG 1 cut(s) 128
BveI ACCTGC 1 cut(s) 68
Cac8I GCNNGC 1 cut(s) 119
Cfr13I GGNCC 1 cut(s) 44
Csp6I GTAC 1 cut(s) 100
CviAII CATG 1 cut(s) 109
CviJI RGCY 4 cut(s) 39, 45, 153, 169
CviKI_1 RGCY 4 cut(s) 39, 45, 153, 169
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 1 cut(s) 33
DpnI GATC 3 cut(s) 52, 92, 107
DpnII GATC 3 cut(s) 50, 90, 105
Eco130I CCWWGG 1 cut(s) 141
EcoRII CCWGG 1 cut(s) 46
EcoT14I CCWWGG 1 cut(s) 141
ErhI CCWWGG 1 cut(s) 141
FaeI CATG 1 cut(s) 112
FaiI YATR 1 cut(s) 110
FatI CATG 1 cut(s) 108
FauI CCCGC 1 cut(s) 62
Fnu4HI GCNGC 2 cut(s) 115, 133
Fsp4HI GCNGC 2 cut(s) 115, 133
GluI GCNGC 2 cut(s) 115, 133
HaeIII GGCC 1 cut(s) 45
Hin1II CATG 1 cut(s) 112
HincII GTYRAC 1 cut(s) 176
HindII GTYRAC 1 cut(s) 176
HphI GGTGA 2 cut(s) 20, 175
Hpy166II GTNNAC 1 cut(s) 176
Hpy188III TCNNGA 1 cut(s) 19
Hpy8I GTNNAC 1 cut(s) 176
HpyCH4IV ACGT 1 cut(s) 15
HpyCH4V TGCA 1 cut(s) 117
HpyF10VI GCNNNNNNNGC 2 cut(s) 123, 132
HpyF3I CTNAG 1 cut(s) 33
HpySE526I ACGT 1 cut(s) 15
Hsp92II CATG 1 cut(s) 112
Kzo9I GATC 3 cut(s) 50, 90, 105
LpnPI CCDG 5 cut(s) 4, 33, 60, 73, 103
Lsp1109I GCAGC 2 cut(s) 101, 144
MaeII ACGT 1 cut(s) 15
MalI GATC 3 cut(s) 52, 92, 107
MboI GATC 3 cut(s) 50, 90, 105
MflI RGATCY 1 cut(s) 50
MhlI GDGCHC 1 cut(s) 9
MmeI TCCRAC 1 cut(s) 36
MnlI CCTC 2 cut(s) 90, 159
MseI TTAA 1 cut(s) 159
MslI CAYNNNNRTG 1 cut(s) 161
MspR9I CCNGG 1 cut(s) 48
MvaI CCWGG 1 cut(s) 48
MwoI GCNNNNNNNGC 2 cut(s) 123, 132
NdeII GATC 3 cut(s) 50, 90, 105
NlaIII CATG 1 cut(s) 112
NlaIV GGNNCC 1 cut(s) 52
PaqCI CACCTGC 1 cut(s) 68
PflMI CCANNNNNTGG 1 cut(s) 18
PkrI GCNGC 2 cut(s) 116, 134
Psp6I CCWGG 1 cut(s) 46
PspGI CCWGG 1 cut(s) 46
PspN4I GGNNCC 1 cut(s) 52
PspPI GGNCC 1 cut(s) 44
PstI CTGCAG 1 cut(s) 119
PsuI RGATCY 1 cut(s) 50
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
RseI CAYNNNNRTG 1 cut(s) 161
SaqAI TTAA 1 cut(s) 159
SatI GCNGC 2 cut(s) 115, 133
Sau3AI GATC 3 cut(s) 50, 90, 105
Sau96I GGNCC 1 cut(s) 44
ScrFI CCNGG 1 cut(s) 48
SduI GDGCHC 1 cut(s) 9
SetI ASST 5 cut(s) 18, 41, 62, 165, 181
SfcI CTRYAG 1 cut(s) 115
SmiMI CAYNNNNRTG 1 cut(s) 161
SsiI CCGC 3 cut(s) 55, 94, 130
StyD4I CCNGG 1 cut(s) 46
StyI CCWWGG 1 cut(s) 141
TaiI ACGT 1 cut(s) 18
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TscAI CASTG 1 cut(s) 128
TseI GCWGC 2 cut(s) 114, 132
TspDTI ATGAA 1 cut(s) 145
TspRI CASTG 1 cut(s) 128
Van91I CCANNNNNTGG 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.