Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0006157

Physical Location & Seq

Genomic Coordinates Forward (+)

8201992 .. 8207084

Gene Structure 5093 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1097400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2163 bp

ATGCAAGGGCAGAGGGGTACTATTGGTTCCTTGCCTGAATTCCTAGATTTTGACCATGGAAGTGCATCAAACGATGCTGCTCTAGATCAGCAAATTTGCTGGAATAGCACACGGAATCCACGGAATCCTGCAGAAAGGATTCCGGACTATGTACAATCACCCGGTGATATGAATGTTGGGTTTGTGAATTCTATGGGACAGGAGCGACAACACTTGAGTAGATGGTGCCTAGGTGAACCTAGTTCTAGCAACTCACAGAGTGAGGCAAGTACTGATGAGCGGAAACCAGATCTTGGATGGTCATCTTCAGTGGATCCTTGTGTAGAAGCTGCTTCAAGAATAGAGGAGCGACACTATGAATCAACTAATAACCTTTCACTGGACAACAATGTGAATACTTTTTTTACACAAAGTTCCAGTTCTGATGCAATCCCACAGAATCTGAACTTGAATGCAGGTTTTGCGGGCCATGGTGGTGATAATAGTCAAGTAATGGAATGTCCTAACACATTCAAGTATAATGTTTCTGAAAATGAGCAAATTCGCCCTCCTAGCAGTTCTAATTCCTTTATGCTTCCTTCTGGAAGTGCTGGATTTTTAATGGGAGAGAATGATGGCAGATCGGGTAGTTCATTGGAGGGGCGTCGTGTATCTTGTAAAAGAAAGGCCATGGAGGGAAATATAGGGCAGTCTTCTCTAAGTGGAAGTTGCAGTTACTTTCAGCATACAGACAGTGGTGGAAGGACTGTTCCTGCTCACTATAATGCAGTCAGCAGCCTTAGCATATCTACTCCATCAGAACCGGGGAATTCAAGACTTGGAGTAGTTGGGGGAGCACTTACTTCCGACAGCCTTCCTGATTCAAATGTTGGAAGAAGCTCAGAAAGCACTCATAGGAACTTCCGTGTGAGGATAAACCCTTCAAATCAGCAAAATTCTATACACTCTAATAGGTTCTTGACAGGGAGGGCTGCTAGGCATTCTAGTATTTCCTCTTCGAATCATTCACCAAGTCTTCTTCCAGTTGATCATAATATGGACCTGAGACCAGCACCAGCTCTAGATAATATGAGTTCACAGAACCAGCCAGTTGTCATCCATGTTCCTGCTTTGCCGCAAAATGTGCAATCGTTGAGATGGAGTGGAGGGTCTAGCTCAAGAACTGGCAGCTTATCAAATTCTGTGGTTTATGGAGACAGAGATGCTCCACAGCCTCCACCACGTGAGGAAGGGAGCTCAAGAAGCATGGCCAGACACATTTTGGATCATCCTATTTTCGTACCAGCAACTGAATTGAGAAATTCAGCTCGGCATCCTGCAGGTGCATCTAACAGAAATTTGCCTGGTGGAAATGCAAGTATTCCTGGTAATGTTGCTTCAGCATCCCGGACTGGCACAAGTTCAGGTGTTAATCCAGCAGCTGCACCTACGTGGGTTCCTCCTCACAATTCTCATCCGCAATTTCCTCGAAGATTATCTGAATATGTTCGCCGATCGTTATTTTCTGCTTCTGGTAGTGAGCCTGGGAGTCAGGGCACTAATTATTTGCCTCTGCATTCGGGTCCTGCATCATCACCAGAGGTTGAGAGTTCATCTGGTACTGGTAACCAAAGTCATCATCGGTCTCACCCAAGGTCAGCATCTTGGATGGAGAGACATGGCGATGGTGGACTTGGACTTCCATATTCATTACGGACTTTGGCTGCTGCAGGCGGTGAAGGAAGTAGCAGGCTGGTATCTGAGATTTGCAATGTCCTGGGTCTCATGCGAAGGGGCGAGAGCTTGCGATTTGAGGATGTTATGATTCTTGATCAGTCAGTCCTTTTTGGGGTGGCAGATATTCACGACCGACATAGAGACATGCGACTTGATGTTGATAACATGTCTTATGAGGAATTATTGGCTCTGGAAGAGCGTATCGGAAACGTGAACACTGGATTAAGTGAAGAAACCATATCAAAGCGCTTGAAACATAAGAAGTATATTGCAGTAGGATCTTCGGCAGATACAGAGCCATGCTGTGTATGCCAGGAGGAATACAATGGTGGAGAAGATCTTGGAACGCTCGAATGCGGACATGATTTCCACAGCGAGTGCATAAAACAATGGCTGATGCACAAGAATTTGTGCCCCATCTGTAAAACTACAGCCCTGGCAACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008285 GO:0009056 GO:0009057 GO:0009791 GO:0009908 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0019941 GO:0022414 GO:0030163 GO:0031624 GO:0032446 GO:0032501 GO:0032502 GO:0036211 GO:0040008 GO:0042127 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044390 GO:0045926 GO:0046620 GO:0046621 GO:0048367 GO:0048437 GO:0048518 GO:0048519 GO:0048523 GO:0048608 GO:0048638 GO:0048640 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051094 GO:0051239 GO:0051240 GO:0051241 GO:0051603 GO:0051865 GO:0061458 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0090567 GO:0099402 GO:0140096 GO:1900055 GO:1900057 GO:1901564 GO:1901565 GO:1901575 GO:1905623 GO:2000024 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

721

Amino Acids

77.59

Weight (kDa)

6.11

Isoelectric Point (pI)

66.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-rbx1	PF12678	670 - 714	1e-08	RING-H2 zinc finger domain
zf-RING_2	PF13639	673 - 714	7.3e-12	Ring finger domain
zf-C3HC4_2	PF13923	673 - 713	7.2e-08	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	673 - 713	5.1e-06	Zinc finger, C3HC4 type (RING finger)
zf-RING_11	PF17123	673 - 700	9.1e-06	RING-like zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0006157)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G45180 AT1G45180 AT1G45180 AT1G45180 AT5G42940 AT5G42940
fragaria_vesca	FvH4_2g34090 FvH4_2g34090
malus_domestica	MD08G1097400.v1.1 MD15G1079700.v1.1
prunus_persica	Prupe.1G432700_v2.0.a1
pyrus_communis	pycom08g07950 pycom15g07410 pycom15g07420
rosa_chinensis	RchiOBHm_Chr6g0308261
rosa_laevigata	RLG00000010653
rosa_multiflora	Rmu_ssc0000355.1_g000008
rosa_roxburghii	Rroxscaffold_7G00160310
rosa_rugosa	Rorug06G0365500 Rorug06G0365600 Rorug06G0365700 Rorug06G0365800
rosa_samantha	Rh6AG474500 Rh6BG485200 Rh6CG490200 Rh6DG476600
rosa_wichuraiana	Rw6G041390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1310
Acc36I	ACCTGC	2 cut(s)	446, 1310
AccB1I	GGYRCC	1 cut(s)	225
AccB7I	CCANNNNNTGG	1 cut(s)	293
AccBSI	CCGCTC	1 cut(s)	280
AccIII	TCCGGA	1 cut(s)	142
AciI	CCGC	6 cut(s)	280, 464, 1115, 1457, 1713, 2073
AclWI	GGATC	4 cut(s)	308, 321, 1272, 2002
AcoI	YGGCCR	1 cut(s)	1248
AcuI	CTGAAG	2 cut(s)	291, 1362
AcvI	CACGTG	1 cut(s)	1223
AcyI	GRCGYC	1 cut(s)	643
AdeI	CACNNNGTG	3 cut(s)	164, 260, 1223
AfaI	GTAC	5 cut(s)	19, 153, 271, 1281, 1600
AfeI	AGCGCT	1 cut(s)	1964
AfiI	CCNNNNNNNGG	3 cut(s)	293, 379, 1828
AflIII	ACRYGT	1 cut(s)	1881
AgsI	TTSAA	7 cut(s)	336, 451, 514, 813, 864, 924, 1969
AjnI	CCWGG	6 cut(s)	1342, 1363, 1522, 1755, 2028, 2151
AleI	CACNNNNGTG	1 cut(s)	1429
AloI	GAACNNNNNNTCC	2 cut(s)	732, 764
AluBI	AGCT	9 cut(s)	329, 879, 1058, 1155, 1170, 1236, 1307, 1421, 1782
AluI	AGCT	9 cut(s)	329, 879, 1058, 1155, 1170, 1236, 1307, 1421, 1782
Alw21I	GWGCWC	2 cut(s)	838, 1238
Alw26I	GTCTC	6 cut(s)	1039, 1188, 1629, 1648, 1766, 1851
AlwI	GGATC	4 cut(s)	308, 321, 1272, 2002
AlwNI	CAGNNNCTG	3 cut(s)	442, 1289, 1421
Aor13HI	TCCGGA	1 cut(s)	142
Aor51HI	AGCGCT	1 cut(s)	1964
AoxI	GGCC	3 cut(s)	466, 666, 1248
ApeKI	GCWGC	9 cut(s)	77, 329, 774, 971, 1167, 1418, 1421, 1703, 1706
Asp700I	GAANNNNTTC	2 cut(s)	138, 1485
AspA2I	CCTAGG	1 cut(s)	229
AspLEI	GCGC	1 cut(s)	1965
AspS9I	GGNCC	3 cut(s)	466, 1039, 1562
AsuC2I	CCSGG	3 cut(s)	162, 804, 1387
AsuHPI	GGTGA	8 cut(s)	150, 176, 245, 488, 999, 1566, 1619, 1727
AsuII	TTCGAA	1 cut(s)	998
AvaII	GGWCC	2 cut(s)	1039, 1562
AvrII	CCTAGG	1 cut(s)	229
BaeGI	GKGCMC	2 cut(s)	1538, 2132
BalI	TGGCCA	1 cut(s)	1250
BamHI	GGATCC	1 cut(s)	313
BanI	GGYRCC	1 cut(s)	225
BanII	GRGCYC	1 cut(s)	1238
BarI	GAAGNNNNNNTAC	4 cut(s)	979, 1011, 1360, 1392
BbrPI	CACGTG	1 cut(s)	1223
BbsI	GAAGAC	2 cut(s)	684, 1007
Bbv12I	GWGCWC	2 cut(s)	838, 1238
BbvI	GCAGC	9 cut(s)	64, 316, 786, 958, 1179, 1408, 1430, 1690, 1693
BccI	CCATC	8 cut(s)	216, 291, 608, 802, 1131, 1642, 1658, 2141
BcgI	CGANNNNNNTGC	2 cut(s)	1448, 1482
BciT130I	CCWGG	6 cut(s)	1344, 1365, 1524, 1757, 2030, 2153
BclI	TGATCA	2 cut(s)	1027, 1810
BcnI	CCSGG	3 cut(s)	162, 804, 1387
BcoDI	GTCTC	6 cut(s)	1039, 1188, 1629, 1648, 1766, 1851
BfmI	CTRYAG	4 cut(s)	129, 1317, 1707, 2145
BfoI	RGCGCY	1 cut(s)	1966
BfuAI	ACCTGC	2 cut(s)	446, 1310
BglII	AGATCT	2 cut(s)	289, 2053
BlnI	CCTAGG	1 cut(s)	229
BmcAI	AGTACT	1 cut(s)	271
Bme1390I	CCNGG	9 cut(s)	162, 804, 1344, 1365, 1387, 1524, 1757, 2030, 2153
Bme18I	GGWCC	2 cut(s)	1039, 1562
BmgT120I	GGNCC	3 cut(s)	466, 1039, 1562
BmiI	GGNNCC	5 cut(s)	28, 227, 315, 1437, 1563
BmrFI	CCNGG	9 cut(s)	162, 804, 1344, 1365, 1387, 1524, 1757, 2030, 2153
BpiI	GAAGAC	2 cut(s)	684, 1007
Bpu10I	CCTNAGC	1 cut(s)	779
Bpu14I	TTCGAA	1 cut(s)	998
BpuEI	CTTGAG	3 cut(s)	235, 1141, 1222
BpuMI	CCSGG	3 cut(s)	162, 804, 1387
BsaAI	YACGTR	2 cut(s)	1223, 1431
BsaBI	GATNNNNATC	1 cut(s)	301
BsaHI	GRCGYC	1 cut(s)	643
BsaI	GGTCTC	3 cut(s)	1039, 1629, 1766
BsaWI	WCCGGW	1 cut(s)	142
Bsc4I	CCNNNNNNNGG	3 cut(s)	293, 379, 1828
Bse1I	ACTGG	8 cut(s)	384, 417, 1022, 1088, 1168, 1396, 1606, 1939
Bse3DI	GCAATG	1 cut(s)	1756
Bse8I	GATNNNNATC	1 cut(s)	301
BseAI	TCCGGA	1 cut(s)	142
BseBI	CCWGG	6 cut(s)	1344, 1365, 1524, 1757, 2030, 2153
BseGI	GGATG	8 cut(s)	302, 1095, 1267, 1312, 1382, 1453, 1653, 1801
BseJI	GATNNNNATC	1 cut(s)	301
BseLI	CCNNNNNNNGG	3 cut(s)	293, 379, 1828
BseMI	GCAATG	1 cut(s)	1756
BseMII	CTCAG	3 cut(s)	894, 1034, 1731
BseNI	ACTGG	8 cut(s)	384, 417, 1022, 1088, 1168, 1396, 1606, 1939
BseRI	GAGGAG	2 cut(s)	359, 1431
BseSI	GKGCMC	2 cut(s)	1538, 2132
BseXI	GCAGC	9 cut(s)	64, 316, 786, 958, 1179, 1408, 1430, 1690, 1693
BsgI	GTGCAG	1 cut(s)	1407
Bsh1285I	CGRYCG	2 cut(s)	1496, 1849
BshFI	GGCC	3 cut(s)	468, 668, 1250
BshNI	GGYRCC	1 cut(s)	225
BsiEI	CGRYCG	2 cut(s)	1496, 1849
BsiHKAI	GWGCWC	2 cut(s)	838, 1238
BsiSI	CCGG	4 cut(s)	143, 162, 803, 1387
BslFI	GGGAC	1 cut(s)	210
BslI	CCNNNNNNNGG	3 cut(s)	293, 379, 1828
BsmAI	GTCTC	6 cut(s)	1039, 1188, 1629, 1648, 1766, 1851
BsmFI	GGGAC	1 cut(s)	210
BsmI	GAATGC	4 cut(s)	457, 979, 1555, 2075
BsnI	GGCC	3 cut(s)	468, 668, 1250
Bso31I	GGTCTC	3 cut(s)	1039, 1629, 1766
Bsp119I	TTCGAA	1 cut(s)	998
Bsp1286I	GDGCHC	4 cut(s)	838, 1238, 1538, 2132
Bsp13I	TCCGGA	1 cut(s)	142
Bsp1407I	TGTACA	1 cut(s)	151
Bsp19I	CCATGG	3 cut(s)	55, 469, 669
BspACI	CCGC	6 cut(s)	280, 464, 1115, 1457, 1713, 2073
BspANI	GGCC	3 cut(s)	468, 668, 1250
BspCNI	CTCAG	3 cut(s)	893, 1035, 1732
BspEI	TCCGGA	1 cut(s)	142
BspLI	GGNNCC	5 cut(s)	28, 227, 315, 1437, 1563
BspMAI	CTGCAG	3 cut(s)	133, 1321, 1711
BspMI	ACCTGC	2 cut(s)	446, 1310
BspPI	GGATC	4 cut(s)	308, 321, 1272, 2002
BspQI	GCTCTTC	1 cut(s)	1905
BspT104I	TTCGAA	1 cut(s)	998
BspT107I	GGYRCC	1 cut(s)	225
BspTNI	GGTCTC	3 cut(s)	1039, 1629, 1766
BsrBI	CCGCTC	1 cut(s)	280
BsrDI	GCAATG	1 cut(s)	1756
BsrGI	TGTACA	1 cut(s)	151
BsrI	ACTGG	8 cut(s)	384, 417, 1022, 1088, 1168, 1396, 1606, 1939
BssNI	GRCGYC	1 cut(s)	643
BssT1I	CCWWGG	5 cut(s)	55, 229, 469, 669, 1631
Bst2UI	CCWGG	6 cut(s)	1344, 1365, 1524, 1757, 2030, 2153
Bst4CI	ACNGT	2 cut(s)	734, 748
Bst6I	CTCTTC	2 cut(s)	1000, 1905
BstACI	GRCGYC	1 cut(s)	643
BstAPI	GCANNNNNTGC	2 cut(s)	461, 1123
BstAUI	TGTACA	1 cut(s)	151
BstBAI	YACGTR	2 cut(s)	1223, 1431
BstBI	TTCGAA	1 cut(s)	998
BstC8I	GCNNGC	4 cut(s)	466, 1711, 1730, 1784
BstDEI	CTNAG	5 cut(s)	698, 779, 880, 1043, 1740
BstDSI	CCRYGG	4 cut(s)	55, 119, 469, 669
BstEII	GGTNACC	1 cut(s)	1604
BstF5I	GGATG	8 cut(s)	302, 1095, 1267, 1312, 1382, 1453, 1653, 1801
BstH2I	RGCGCY	1 cut(s)	1966
BstHHI	GCGC	1 cut(s)	1965
BstMAI	GTCTC	6 cut(s)	1039, 1188, 1629, 1648, 1766, 1851
BstMCI	CGRYCG	2 cut(s)	1496, 1849
BstMWI	GCNNNNNNNGC	8 cut(s)	105, 461, 552, 780, 885, 1123, 1242, 2025
BstNI	CCWGG	6 cut(s)	1344, 1365, 1524, 1757, 2030, 2153
BstNSI	RCATGY	2 cut(s)	1864, 1885
BstPI	GGTNACC	1 cut(s)	1604
BstSCI	CCNGG	9 cut(s)	160, 802, 1342, 1363, 1385, 1522, 1755, 2028, 2151
BstSFI	CTRYAG	4 cut(s)	129, 1317, 1707, 2145
BstSLI	GKGCMC	2 cut(s)	1538, 2132
BstV1I	GCAGC	9 cut(s)	64, 316, 786, 958, 1179, 1408, 1430, 1690, 1693
BstV2I	GAAGAC	2 cut(s)	684, 1007
BstX2I	RGATCY	4 cut(s)	289, 313, 1994, 2053
BstYI	RGATCY	4 cut(s)	289, 313, 1994, 2053
BsuRI	GGCC	3 cut(s)	468, 668, 1250
BtgI	CCRYGG	4 cut(s)	55, 119, 469, 669
BtgZI	GCGATG	1 cut(s)	1677
BtsCI	GGATG	8 cut(s)	302, 1095, 1267, 1312, 1382, 1453, 1653, 1801
BtsIMutI	CAGTG	4 cut(s)	315, 377, 739, 1932
BveI	ACCTGC	2 cut(s)	446, 1310
Cac8I	GCNNGC	4 cut(s)	466, 1711, 1730, 1784
CaiI	CAGNNNCTG	3 cut(s)	442, 1289, 1421
CfoI	GCGC	1 cut(s)	1965
Cfr13I	GGNCC	3 cut(s)	466, 1039, 1562
CseI	GACGC	1 cut(s)	632
Csp6I	GTAC	5 cut(s)	18, 152, 270, 1280, 1599
CspCI	CAANNNNNGTGG	2 cut(s)	1164, 1199
CviQI	GTAC	5 cut(s)	18, 152, 270, 1280, 1599
DdeI	CTNAG	5 cut(s)	698, 779, 880, 1043, 1740
DraIII	CACNNNGTG	3 cut(s)	164, 260, 1223
EaeI	YGGCCR	1 cut(s)	1248
Eam1104I	CTCTTC	2 cut(s)	1000, 1905
EarI	CTCTTC	2 cut(s)	1000, 1905
Ecl136II	GAGCTC	1 cut(s)	1236
Eco130I	CCWWGG	5 cut(s)	55, 229, 469, 669, 1631
Eco24I	GRGCYC	1 cut(s)	1238
Eco31I	GGTCTC	3 cut(s)	1039, 1629, 1766
Eco47I	GGWCC	2 cut(s)	1039, 1562
Eco47III	AGCGCT	1 cut(s)	1964
Eco53kI	GAGCTC	1 cut(s)	1236
Eco57I	CTGAAG	2 cut(s)	291, 1362
Eco72I	CACGTG	1 cut(s)	1223
Eco91I	GGTNACC	1 cut(s)	1604
EcoICRI	GAGCTC	1 cut(s)	1236
EcoO109I	RGGNCCY	1 cut(s)	1562
EcoO65I	GGTNACC	1 cut(s)	1604
EcoRI	GAATTC	3 cut(s)	38, 187, 808
EcoRII	CCWGG	6 cut(s)	1342, 1363, 1522, 1755, 2028, 2151
EcoT14I	CCWWGG	5 cut(s)	55, 229, 469, 669, 1631
EcoT38I	GRGCYC	1 cut(s)	1238
ErhI	CCWWGG	5 cut(s)	55, 229, 469, 669, 1631
FaqI	GGGAC	1 cut(s)	210
FauI	CCCGC	1 cut(s)	457
FbaI	TGATCA	2 cut(s)	1027, 1810
FokI	GGATG	8 cut(s)	309, 1082, 1254, 1299, 1369, 1440, 1660, 1808
FriOI	GRGCYC	1 cut(s)	1238
GlaI	GCGC	1 cut(s)	1964
HaeII	RGCGCY	1 cut(s)	1966
HaeIII	GGCC	3 cut(s)	468, 668, 1250
HapII	CCGG	4 cut(s)	143, 162, 803, 1387
HgaI	GACGC	1 cut(s)	632
HhaI	GCGC	1 cut(s)	1965
Hin1I	GRCGYC	1 cut(s)	643
Hin6I	GCGC	1 cut(s)	1963
HinP1I	GCGC	1 cut(s)	1963
HinfI	GANTC	9 cut(s)	115, 124, 139, 359, 439, 860, 1000, 1528, 1804
HpaII	CCGG	4 cut(s)	143, 162, 803, 1387
HphI	GGTGA	8 cut(s)	150, 176, 245, 488, 999, 1566, 1619, 1727
Hpy166II	GTNNAC	4 cut(s)	236, 1076, 1670, 1930
Hpy188I	TCNGA	9 cut(s)	424, 444, 529, 799, 847, 883, 1480, 1741, 1922
Hpy8I	GTNNAC	4 cut(s)	236, 1076, 1670, 1930
Hpy99I	CGWCG	1 cut(s)	648
HpyAV	CCTTC	7 cut(s)	588, 735, 863, 930, 1223, 1712, 1764
HpyCH4III	ACNGT	2 cut(s)	734, 748
HpyCH4IV	ACGT	3 cut(s)	1222, 1430, 1926
HpyF10VI	GCNNNNNNNGC	8 cut(s)	105, 461, 552, 780, 885, 1123, 1242, 2025
HpyF3I	CTNAG	5 cut(s)	698, 779, 880, 1043, 1740
HpySE526I	ACGT	3 cut(s)	1222, 1430, 1926
Hsp92I	GRCGYC	1 cut(s)	643
HspAI	GCGC	1 cut(s)	1963
Kpn2I	TCCGGA	1 cut(s)	142
Ksp22I	TGATCA	2 cut(s)	1027, 1810
LguI	GCTCTTC	1 cut(s)	1905
LmnI	GCTCC	5 cut(s)	202, 346, 833, 1210, 1233
Lsp1109I	GCAGC	9 cut(s)	64, 316, 786, 958, 1179, 1408, 1430, 1690, 1693
MaeII	ACGT	3 cut(s)	1222, 1430, 1926
MaeIII	GTNAC	2 cut(s)	713, 1604
MbiI	CCGCTC	1 cut(s)	280
MflI	RGATCY	4 cut(s)	289, 313, 1994, 2053
MhlI	GDGCHC	4 cut(s)	838, 1238, 1538, 2132
MlsI	TGGCCA	1 cut(s)	1250
MluNI	TGGCCA	1 cut(s)	1250
MlyI	GAGTC	1 cut(s)	1537
MmeI	TCCRAC	2 cut(s)	850, 870
Mox20I	TGGCCA	1 cut(s)	1250
MroI	TCCGGA	1 cut(s)	142
MroXI	GAANNNNTTC	2 cut(s)	138, 1485
MscI	TGGCCA	1 cut(s)	1250
MseI	TTAA	3 cut(s)	599, 1410, 1940
MslI	CAYNNNNRTG	5 cut(s)	60, 474, 762, 1429, 1662
Msp20I	TGGCCA	1 cut(s)	1250
MspA1I	CMGCKG	1 cut(s)	1421
MspI	CCGG	4 cut(s)	143, 162, 803, 1387
MspR9I	CCNGG	9 cut(s)	162, 804, 1344, 1365, 1387, 1524, 1757, 2030, 2153
Mva1269I	GAATGC	4 cut(s)	457, 979, 1555, 2075
MvaI	CCWGG	6 cut(s)	1344, 1365, 1524, 1757, 2030, 2153
MwoI	GCNNNNNNNGC	8 cut(s)	105, 461, 552, 780, 885, 1123, 1242, 2025
NciI	CCSGG	3 cut(s)	162, 804, 1387
NcoI	CCATGG	3 cut(s)	55, 469, 669
NlaIV	GGNNCC	5 cut(s)	28, 227, 315, 1437, 1563
NmeAIII	GCCGAG	1 cut(s)	1288
NspI	RCATGY	2 cut(s)	1864, 1885
NspV	TTCGAA	1 cut(s)	998
OliI	CACNNNNGTG	1 cut(s)	1429
PaqCI	CACCTGC	1 cut(s)	1310
PciI	ACATGT	1 cut(s)	1881
PciSI	GCTCTTC	1 cut(s)	1905
PctI	GAATGC	4 cut(s)	457, 979, 1555, 2075
PdmI	GAANNNNTTC	2 cut(s)	138, 1485
PfeI	GAWTC	8 cut(s)	115, 124, 139, 359, 439, 860, 1000, 1804
PflMI	CCANNNNNTGG	1 cut(s)	293
PfoI	TCCNGGA	1 cut(s)	1385
Ple19I	CGATCG	1 cut(s)	1496
PleI	GAGTC	1 cut(s)	1536
PmaCI	CACGTG	1 cut(s)	1223
PmlI	CACGTG	1 cut(s)	1223
PpsI	GAGTC	1 cut(s)	1536
Ppu21I	YACGTR	2 cut(s)	1223, 1431
PpuMI	RGGWCCY	1 cut(s)	1562
PscI	ACATGT	1 cut(s)	1881
Psp124BI	GAGCTC	1 cut(s)	1238
Psp5II	RGGWCCY	1 cut(s)	1562
Psp6I	CCWGG	6 cut(s)	1342, 1363, 1522, 1755, 2028, 2151
PspCI	CACGTG	1 cut(s)	1223
PspEI	GGTNACC	1 cut(s)	1604
PspGI	CCWGG	6 cut(s)	1342, 1363, 1522, 1755, 2028, 2151
PspN4I	GGNNCC	5 cut(s)	28, 227, 315, 1437, 1563
PspPI	GGNCC	3 cut(s)	466, 1039, 1562
PspPPI	RGGWCCY	1 cut(s)	1562
PsrI	GAACNNNNNNTAC	2 cut(s)	10, 42
PstI	CTGCAG	3 cut(s)	133, 1321, 1711
PstNI	CAGNNNCTG	3 cut(s)	442, 1289, 1421
PsuI	RGATCY	4 cut(s)	289, 313, 1994, 2053
PvuI	CGATCG	1 cut(s)	1496
PvuII	CAGCTG	1 cut(s)	1421
RsaI	GTAC	5 cut(s)	19, 153, 271, 1281, 1600
RsaNI	GTAC	5 cut(s)	18, 152, 270, 1280, 1599
RseI	CAYNNNNRTG	5 cut(s)	60, 474, 762, 1429, 1662
SacI	GAGCTC	1 cut(s)	1238
SapI	GCTCTTC	1 cut(s)	1905
SaqAI	TTAA	3 cut(s)	599, 1410, 1940
Sau96I	GGNCC	3 cut(s)	466, 1039, 1562
SbfI	CCTGCAGG	1 cut(s)	1321
ScaI	AGTACT	1 cut(s)	271
SchI	GAGTC	1 cut(s)	1537
ScrFI	CCNGG	9 cut(s)	162, 804, 1344, 1365, 1387, 1524, 1757, 2030, 2153
SdaI	CCTGCAGG	1 cut(s)	1321
SduI	GDGCHC	4 cut(s)	838, 1238, 1538, 2132
SfcI	CTRYAG	4 cut(s)	129, 1317, 1707, 2145
SfuI	TTCGAA	1 cut(s)	998
SinI	GGWCC	2 cut(s)	1039, 1562
SmiMI	CAYNNNNRTG	5 cut(s)	60, 474, 762, 1429, 1662
SmlI	CTYRAG	3 cut(s)	214, 1156, 1237
SmoI	CTYRAG	3 cut(s)	214, 1156, 1237
Sse8387I	CCTGCAGG	1 cut(s)	1321
SsiI	CCGC	6 cut(s)	280, 464, 1115, 1457, 1713, 2073
SstI	GAGCTC	1 cut(s)	1238
StyD4I	CCNGG	9 cut(s)	160, 802, 1342, 1363, 1385, 1522, 1755, 2028, 2151
StyI	CCWWGG	5 cut(s)	55, 229, 469, 669, 1631
TaaI	ACNGT	2 cut(s)	734, 748
TaiI	ACGT	3 cut(s)	1225, 1433, 1929
TaqI	TCGA	3 cut(s)	998, 1468, 2067
TaqII	GACCGA	2 cut(s)	1611, 1863
TatI	WGTACW	2 cut(s)	151, 269
TauI	GCSGC	1 cut(s)	1117
TfiI	GAWTC	8 cut(s)	115, 124, 139, 359, 439, 860, 1000, 1804
Tru1I	TTAA	3 cut(s)	599, 1410, 1940
Tru9I	TTAA	3 cut(s)	599, 1410, 1940
TscAI	CASTG	4 cut(s)	315, 384, 739, 1939
TseI	GCWGC	9 cut(s)	77, 329, 774, 971, 1167, 1418, 1421, 1703, 1706
TspDTI	ATGAA	5 cut(s)	185, 372, 621, 1581, 1677
TspGWI	ACGGA	4 cut(s)	127, 136, 893, 1708
TspRI	CASTG	4 cut(s)	315, 384, 739, 1939
Van91I	CCANNNNNTGG	1 cut(s)	293
VpaK11BI	GGWCC	2 cut(s)	1039, 1562
XbaI	TCTAGA	2 cut(s)	82, 1060
XceI	RCATGY	2 cut(s)	1864, 1885
XcmI	CCANNNNNNNNNTGG	2 cut(s)	294, 1258
XmaJI	CCTAGG	1 cut(s)	229
XmnI	GAANNNNTTC	2 cut(s)	138, 1485
ZrmI	AGTACT	1 cut(s)	271

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.