Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0006157

Physical Location & Seq

Genomic Coordinates Forward (+)

5427884 .. 5432806

Gene Structure 4923 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1079700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2160 bp

ATGCAAGGGCAGAGGGGTACTATCGGTTCCTTGCCTGAAATGCTAGATTTTGACCATGGAAGTGCATCAAACGATGCTGCTCTAGATCAGCAAATTTGCTGGAATAGCACACGGAATCCTGCTGAAAGGATTCCGGACTATGTACAATCACCTGGTGATGTGAATGTTGGGTTTGTGAATTCTATGGGACAGGAGCGACAACACTTGAGTAGATGGTGCCTGGGTGAACCTAGTTCTAGCAACTCACAGAGTGAGGTTAGTCGTGATGAGCGGAAACCAGAACTTGGATGGTCTTCTTCAGTGGATCCTTGTGTAGAAGCTGCTTCAAGGTTAGAGGAGCGACGCTATGAACCAACTAATAACCTTTCACTGGGCAACAATGTGAATACTATTTTTACACAAAGTTCCAGTTCCAATGCAATTCCACAGAATCTGAACTTGAATGCAGCTTTTGCGGGTCATGGTGGTGATAATAGTCAAGTAATGGAATGTCCTAACACATTCAAGTATAATGTTTCTGAAAATGAGCAAATTCGACCTCCTAGTAGTTCTAATCCCTTTATACTACCTTCTGGAACTGCTGGATTTTTAATGGGAGAGAATGATGGCAGATCGGGCAGTTCATTGGAGGGTCGTCGTGTATCTTGTAAAAGAAAGGCTATGGAGGGAAATATAGGACAGTCTTCTCTAAGTGGAAGTTGCAGTTACTTTCAGCATACAGACAGTGGTGGAAGGACTTCTGTTCCCGCTCACTATAATGCAGGTGGCAGCCTAAGCATATCTACACCATCAGAACAGGGGAATTCAAGACTTGGAGTAGTTGGGAGAGCACTTGCTTCTGACAGCCTTCCTGATTCAAATGCTGGAAGAAGCTCAGAAAGCACTCATAGGAACTTCCGTGTGAGAATAAATCCTTCAAATCAGCAAAATTCTATACCCTCTAATAGGTTCTCAACGGGGAGGGCTGCTAGGCATTCTAGTATTTCCTCTTCGCATCATTCACCAAGTCTTCTTCCAGTTGATCATAATATGGACTTGAGACCAGCACCAGCGCTAGATAATACGAGTTCACAGAACCCGCCAGTTGTCATCCATGTTCCTGCTTTGCCGCAAAATGTGCAATCATTGAGATGGAGTGGAGGGTCTAGCTCAAGAACCAGCAGCTTATCAAATTCTGTGGTTTTTGGAGACAGAGATGCTCCACAGCCTCCACCACTTGAGGAAGGGAGCTCAAGAAGCATGGCGAGACACATTTTGGATCATCCTATTTTTGTACCAGCAACTGAATTGAGAAATTCGGCTCGGCATCCTGCAGGTGCATCTACTAGAAATTTACCTGGTGGAAATGCAAGTATTCCTGGAAATGTTGCTTCAGCATCCCGGACTGGCACAAGTTCAGGTGTTAATCCAGCAGCTGCACCTACGTGGGTTCCTCCTCACAATTCTCATCCGCAATTTCCTCGAAGATTATCTGAATATGTTCGTCGATCATTATTTTCTGCTACTGGTAGTGAGCCTGGGAGCCAGGGCACTAATTATTTGCCTATGCGTTCGGGTCCTGCGTCATCACCAGAGGTTGGGAGTTCATCTGGTACAGGTAACCAGGGTCATCATCAGTCTCACCCAAGGTCAGCATCTTGGATGGAGAGACATGGTGATGGTGGACTTGGGCTTCCGTATTCATTACGGACTTTGGCTGCTGCAGGTGGTGAAGGAAGTAGCAGGCTCGTATCTGAGCAGATTTGCAATGTACTGGGTCTCATGCGTAGGGGCGAGAGCTTGCGATTCGAGGATGTTATGATTCTTGATCAGTCGGTCCTTTTTGGGGTGGCTGATATTCACGACAGACATAGAGACATGCGACTTGATGTTGATAACATGTCTTATGAGGAATTATTGGCTCTGGAAGAGCGTATTGGAAACGTGAACACTGGATTAAGTGAAGAAACCATATCAAAGCGCTTGAAACATAAGAAGTATGTTGCAGTAAGATCTCCGGCAGATACAGAGCCGTGCTGCGTATGCCAGGAGGAATACAATGGTGGAGAAGATCTTGGAACGCTCGAATGCGGACATGATTTCCACAGCGAGTGCATAAAACAATGGCTGATGCACAAGAATTTGTGCCCCATCTGTAAAACTACAGCCTTGGCAGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008285 GO:0009056 GO:0009057 GO:0009791 GO:0009908 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0019941 GO:0022414 GO:0030163 GO:0031624 GO:0032446 GO:0032501 GO:0032502 GO:0036211 GO:0040008 GO:0042127 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044390 GO:0045926 GO:0046620 GO:0046621 GO:0048367 GO:0048437 GO:0048518 GO:0048519 GO:0048523 GO:0048608 GO:0048638 GO:0048640 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051094 GO:0051239 GO:0051240 GO:0051241 GO:0051603 GO:0051865 GO:0061458 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0090567 GO:0099402 GO:0140096 GO:1900055 GO:1900057 GO:1901564 GO:1901565 GO:1901575 GO:1905623 GO:2000024 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

720

Amino Acids

77.28

Weight (kDa)

6.37

Isoelectric Point (pI)

67.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-rbx1	PF12678	670 - 713	2.2e-08	RING-H2 zinc finger domain
zf-RING_2	PF13639	672 - 713	7.3e-12	Ring finger domain
zf-C3HC4_2	PF13923	672 - 712	7.2e-08	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	672 - 712	5.1e-06	Zinc finger, C3HC4 type (RING finger)
zf-RING_11	PF17123	672 - 699	9.1e-06	RING-like zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006157)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G45180 AT1G45180 AT1G45180 AT1G45180 AT5G42940 AT5G42940
fragaria_vesca	FvH4_2g34090 FvH4_2g34090
malus_domestica	MD08G1097400.v1.1 MD15G1079700.v1.1
prunus_persica	Prupe.1G432700_v2.0.a1
pyrus_communis	pycom08g07950 pycom15g07410 pycom15g07420
rosa_chinensis	RchiOBHm_Chr6g0308261
rosa_laevigata	RLG00000010653
rosa_multiflora	Rmu_ssc0000355.1_g000008
rosa_roxburghii	Rroxscaffold_7G00160310
rosa_rugosa	Rorug06G0365500 Rorug06G0365600 Rorug06G0365700 Rorug06G0365800
rosa_samantha	Rh6AG474500 Rh6BG485200 Rh6CG490200 Rh6DG476600
rosa_wichuraiana	Rw6G041390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	3 cut(s)	752, 1304, 1694
Acc36I	ACCTGC	3 cut(s)	752, 1304, 1694
AccB1I	GGYRCC	1 cut(s)	216
AccB7I	CCANNNNNTGG	2 cut(s)	284, 1577
AccBSI	CCGCTC	2 cut(s)	271, 749
AccIII	TCCGGA	1 cut(s)	133
AciI	CCGC	7 cut(s)	271, 455, 747, 1079, 1109, 1451, 2070
AclWI	GGATC	3 cut(s)	299, 312, 1266
AcsI	RAATTY	9 cut(s)	93, 178, 531, 802, 928, 1171, 1294, 1330, 2119
AcuI	CTGAAG	2 cut(s)	282, 1356
AdeI	CACNNNGTG	2 cut(s)	155, 251
AfaI	GTAC	5 cut(s)	19, 144, 1275, 1594, 1752
AfeI	AGCGCT	2 cut(s)	1053, 1961
AfiI	CCNNNNNNNGG	5 cut(s)	284, 370, 945, 1577, 1825
AflIII	ACRYGT	1 cut(s)	1878
AgsI	TTSAA	7 cut(s)	327, 442, 505, 807, 858, 918, 1966
AjnI	CCWGG	8 cut(s)	151, 219, 1336, 1357, 1516, 1524, 1602, 2025
AjuI	GAANNNNNNNTTGG	2 cut(s)	1899, 1931
AleI	CACNNNNGTG	1 cut(s)	1423
AluBI	AGCT	8 cut(s)	320, 449, 873, 1149, 1164, 1230, 1415, 1779
AluI	AGCT	8 cut(s)	320, 449, 873, 1149, 1164, 1230, 1415, 1779
Alw21I	GWGCWC	2 cut(s)	832, 1232
Alw26I	GTCTC	7 cut(s)	1033, 1182, 1240, 1623, 1642, 1763, 1848
AlwI	GGATC	3 cut(s)	299, 312, 1266
AlwNI	CAGNNNCTG	3 cut(s)	433, 1283, 1415
Aor13HI	TCCGGA	1 cut(s)	133
Aor51HI	AGCGCT	2 cut(s)	1053, 1961
ApoI	RAATTY	9 cut(s)	93, 178, 531, 802, 928, 1171, 1294, 1330, 2119
Asp700I	GAANNNNTTC	3 cut(s)	129, 736, 1479
AspLEI	GCGC	2 cut(s)	1054, 1962
AspS9I	GGNCC	2 cut(s)	1556, 1816
AsuC2I	CCSGG	1 cut(s)	1381
AsuHPI	GGTGA	9 cut(s)	141, 167, 236, 479, 993, 1560, 1613, 1667, 1721
AvaII	GGWCC	2 cut(s)	1556, 1816
BaeGI	GKGCMC	2 cut(s)	1532, 2129
BamHI	GGATCC	1 cut(s)	304
BanI	GGYRCC	1 cut(s)	216
BanII	GRGCYC	1 cut(s)	1232
BarI	GAAGNNNNNNTAC	2 cut(s)	973, 1005
BbsI	GAAGAC	3 cut(s)	285, 675, 1001
Bbv12I	GWGCWC	2 cut(s)	832, 1232
BccI	CCATC	8 cut(s)	207, 282, 599, 796, 1125, 1636, 1652, 2138
BceAI	ACGGC	1 cut(s)	1996
BcgI	CGANNNNNNTGC	2 cut(s)	1442, 1476
BciT130I	CCWGG	8 cut(s)	153, 221, 1338, 1359, 1518, 1526, 1604, 2027
BclI	TGATCA	2 cut(s)	1021, 1807
BcnI	CCSGG	1 cut(s)	1381
BcoDI	GTCTC	7 cut(s)	1033, 1182, 1240, 1623, 1642, 1763, 1848
BfmI	CTRYAG	3 cut(s)	1311, 1701, 2142
BfoI	RGCGCY	2 cut(s)	1055, 1963
BfuAI	ACCTGC	3 cut(s)	752, 1304, 1694
BglII	AGATCT	2 cut(s)	1991, 2050
Bme1390I	CCNGG	9 cut(s)	153, 221, 1338, 1359, 1381, 1518, 1526, 1604, 2027
Bme18I	GGWCC	2 cut(s)	1556, 1816
BmgT120I	GGNCC	2 cut(s)	1556, 1816
BmiI	GGNNCC	6 cut(s)	28, 218, 306, 1431, 1523, 1557
BmrFI	CCNGG	9 cut(s)	153, 221, 1338, 1359, 1381, 1518, 1526, 1604, 2027
BmrI	ACTGGG	2 cut(s)	380, 1763
BmsI	GCATC	9 cut(s)	64, 74, 1003, 1186, 1315, 1328, 1385, 1643, 2100
BmuI	ACTGGG	2 cut(s)	380, 1763
BpiI	GAAGAC	3 cut(s)	285, 675, 1001
Bpu10I	CCTNAGC	1 cut(s)	773
BpuEI	CTTGAG	5 cut(s)	226, 1057, 1135, 1216, 1238
BpuMI	CCSGG	1 cut(s)	1381
BsaAI	YACGTR	1 cut(s)	1425
BsaI	GGTCTC	2 cut(s)	1033, 1763
BsaJI	CCNNGG	7 cut(s)	55, 220, 1517, 1525, 1603, 1625, 2148
BsaWI	WCCGGW	1 cut(s)	133
Bsc4I	CCNNNNNNNGG	5 cut(s)	284, 370, 945, 1577, 1825
Bse1I	ACTGG	8 cut(s)	375, 408, 1016, 1082, 1390, 1510, 1758, 1936
Bse3DI	GCAATG	1 cut(s)	1753
BseAI	TCCGGA	1 cut(s)	133
BseBI	CCWGG	8 cut(s)	153, 221, 1338, 1359, 1518, 1526, 1604, 2027
BseDI	CCNNGG	7 cut(s)	55, 220, 1517, 1525, 1603, 1625, 2148
BseGI	GGATG	8 cut(s)	293, 1089, 1261, 1306, 1376, 1447, 1647, 1798
BseLI	CCNNNNNNNGG	5 cut(s)	284, 370, 945, 1577, 1825
BseMI	GCAATG	1 cut(s)	1753
BseMII	CTCAG	2 cut(s)	888, 1725
BseNI	ACTGG	8 cut(s)	375, 408, 1016, 1082, 1390, 1510, 1758, 1936
BseRI	GAGGAG	2 cut(s)	350, 1425
BseSI	GKGCMC	2 cut(s)	1532, 2129
BsgI	GTGCAG	1 cut(s)	1401
BshNI	GGYRCC	1 cut(s)	216
BsiHKAI	GWGCWC	2 cut(s)	832, 1232
BsiSI	CCGG	3 cut(s)	134, 1381, 1997
BslFI	GGGAC	1 cut(s)	201
BslI	CCNNNNNNNGG	5 cut(s)	284, 370, 945, 1577, 1825
BsmAI	GTCTC	7 cut(s)	1033, 1182, 1240, 1623, 1642, 1763, 1848
BsmFI	GGGAC	1 cut(s)	201
BsmI	GAATGC	3 cut(s)	448, 973, 2072
Bso31I	GGTCTC	2 cut(s)	1033, 1763
Bsp1286I	GDGCHC	4 cut(s)	832, 1232, 1532, 2129
Bsp13I	TCCGGA	1 cut(s)	133
Bsp1407I	TGTACA	1 cut(s)	142
Bsp143I	GATC	9 cut(s)	85, 304, 611, 1021, 1258, 1487, 1807, 1991, 2050
Bsp19I	CCATGG	1 cut(s)	55
BspACI	CCGC	7 cut(s)	271, 455, 747, 1079, 1109, 1451, 2070
BspCNI	CTCAG	2 cut(s)	887, 1726
BspEI	TCCGGA	1 cut(s)	133
BspLI	GGNNCC	6 cut(s)	28, 218, 306, 1431, 1523, 1557
BspMAI	CTGCAG	2 cut(s)	1315, 1705
BspMI	ACCTGC	3 cut(s)	752, 1304, 1694
BspPI	GGATC	3 cut(s)	299, 312, 1266
BspQI	GCTCTTC	1 cut(s)	1902
BspT107I	GGYRCC	1 cut(s)	216
BspTNI	GGTCTC	2 cut(s)	1033, 1763
BsrBI	CCGCTC	2 cut(s)	271, 749
BsrDI	GCAATG	1 cut(s)	1753
BsrGI	TGTACA	1 cut(s)	142
BsrI	ACTGG	8 cut(s)	375, 408, 1016, 1082, 1390, 1510, 1758, 1936
BssECI	CCNNGG	7 cut(s)	55, 220, 1517, 1525, 1603, 1625, 2148
BssMI	GATC	9 cut(s)	85, 304, 611, 1021, 1258, 1487, 1807, 1991, 2050
BssT1I	CCWWGG	3 cut(s)	55, 1625, 2148
Bst2UI	CCWGG	8 cut(s)	153, 221, 1338, 1359, 1518, 1526, 1604, 2027
Bst4CI	ACNGT	2 cut(s)	681, 725
Bst6I	CTCTTC	2 cut(s)	994, 1902
BstAPI	GCANNNNNTGC	2 cut(s)	452, 1117
BstAUI	TGTACA	1 cut(s)	142
BstBAI	YACGTR	1 cut(s)	1425
BstC8I	GCNNGC	2 cut(s)	1724, 1781
BstDEI	CTNAG	4 cut(s)	689, 773, 874, 1734
BstDSI	CCRYGG	1 cut(s)	55
BstEII	GGTNACC	1 cut(s)	1598
BstENI	CCTNNNNNAGG	1 cut(s)	943
BstF5I	GGATG	8 cut(s)	293, 1089, 1261, 1306, 1376, 1447, 1647, 1798
BstH2I	RGCGCY	2 cut(s)	1055, 1963
BstHHI	GCGC	2 cut(s)	1054, 1962
BstKTI	GATC	9 cut(s)	88, 307, 614, 1024, 1261, 1490, 1810, 1994, 2053
BstMAI	GTCTC	7 cut(s)	1033, 1182, 1240, 1623, 1642, 1763, 1848
BstMBI	GATC	9 cut(s)	85, 304, 611, 1021, 1258, 1487, 1807, 1991, 2050
BstNI	CCWGG	8 cut(s)	153, 221, 1338, 1359, 1518, 1526, 1604, 2027
BstNSI	RCATGY	2 cut(s)	1861, 1882
BstPI	GGTNACC	1 cut(s)	1598
BstSCI	CCNGG	9 cut(s)	151, 219, 1336, 1357, 1379, 1516, 1524, 1602, 2025
BstSFI	CTRYAG	3 cut(s)	1311, 1701, 2142
BstSLI	GKGCMC	2 cut(s)	1532, 2129
BstV2I	GAAGAC	3 cut(s)	285, 675, 1001
BstX2I	RGATCY	3 cut(s)	304, 1991, 2050
BstYI	RGATCY	3 cut(s)	304, 1991, 2050
BtgI	CCRYGG	1 cut(s)	55
BtsCI	GGATG	8 cut(s)	293, 1089, 1261, 1306, 1376, 1447, 1647, 1798
BtsIMutI	CAGTG	4 cut(s)	306, 368, 730, 1929
BveI	ACCTGC	3 cut(s)	752, 1304, 1694
Cac8I	GCNNGC	2 cut(s)	1724, 1781
CaiI	CAGNNNCTG	3 cut(s)	433, 1283, 1415
CfoI	GCGC	2 cut(s)	1054, 1962
Cfr13I	GGNCC	2 cut(s)	1556, 1816
CseI	GACGC	2 cut(s)	351, 1551
CsiI	ACCWGGT	2 cut(s)	151, 1336
Csp6I	GTAC	5 cut(s)	18, 143, 1274, 1593, 1751
CspCI	CAANNNNNGTGG	2 cut(s)	1158, 1193
CviQI	GTAC	5 cut(s)	18, 143, 1274, 1593, 1751
DdeI	CTNAG	4 cut(s)	689, 773, 874, 1734
DpnI	GATC	9 cut(s)	87, 306, 613, 1023, 1260, 1489, 1809, 1993, 2052
DpnII	GATC	9 cut(s)	85, 304, 611, 1021, 1258, 1487, 1807, 1991, 2050
DraIII	CACNNNGTG	2 cut(s)	155, 251
Eam1104I	CTCTTC	2 cut(s)	994, 1902
EarI	CTCTTC	2 cut(s)	994, 1902
Ecl136II	GAGCTC	1 cut(s)	1230
Eco130I	CCWWGG	3 cut(s)	55, 1625, 2148
Eco24I	GRGCYC	1 cut(s)	1232
Eco31I	GGTCTC	2 cut(s)	1033, 1763
Eco47I	GGWCC	2 cut(s)	1556, 1816
Eco47III	AGCGCT	2 cut(s)	1053, 1961
Eco53kI	GAGCTC	1 cut(s)	1230
Eco57I	CTGAAG	2 cut(s)	282, 1356
Eco91I	GGTNACC	1 cut(s)	1598
EcoICRI	GAGCTC	1 cut(s)	1230
EcoNI	CCTNNNNNAGG	1 cut(s)	943
EcoO109I	RGGNCCY	1 cut(s)	1556
EcoO65I	GGTNACC	1 cut(s)	1598
EcoRI	GAATTC	2 cut(s)	178, 802
EcoRII	CCWGG	8 cut(s)	151, 219, 1336, 1357, 1516, 1524, 1602, 2025
EcoT14I	CCWWGG	3 cut(s)	55, 1625, 2148
EcoT38I	GRGCYC	1 cut(s)	1232
ErhI	CCWWGG	3 cut(s)	55, 1625, 2148
FaqI	GGGAC	1 cut(s)	201
FauI	CCCGC	3 cut(s)	448, 754, 1086
FbaI	TGATCA	2 cut(s)	1021, 1807
FokI	GGATG	8 cut(s)	300, 1076, 1248, 1293, 1363, 1434, 1654, 1805
FriOI	GRGCYC	1 cut(s)	1232
GlaI	GCGC	2 cut(s)	1053, 1961
HaeII	RGCGCY	2 cut(s)	1055, 1963
HapII	CCGG	3 cut(s)	134, 1381, 1997
HgaI	GACGC	2 cut(s)	351, 1551
HhaI	GCGC	2 cut(s)	1054, 1962
Hin6I	GCGC	2 cut(s)	1052, 1960
HinP1I	GCGC	2 cut(s)	1052, 1960
HinfI	GANTC	6 cut(s)	115, 130, 430, 854, 1785, 1801
HpaII	CCGG	3 cut(s)	134, 1381, 1997
HphI	GGTGA	9 cut(s)	141, 167, 236, 479, 993, 1560, 1613, 1667, 1721
Hpy166II	GTNNAC	4 cut(s)	227, 1070, 1664, 1927
Hpy188I	TCNGA	7 cut(s)	435, 520, 793, 841, 877, 1474, 1735
Hpy8I	GTNNAC	4 cut(s)	227, 1070, 1664, 1927
Hpy99I	CGWCG	3 cut(s)	345, 639, 1488
HpyAV	CCTTC	6 cut(s)	579, 726, 857, 924, 1217, 1706
HpyCH4III	ACNGT	2 cut(s)	681, 725
HpyCH4IV	ACGT	2 cut(s)	1424, 1923
HpyF3I	CTNAG	4 cut(s)	689, 773, 874, 1734
HpySE526I	ACGT	2 cut(s)	1424, 1923
HspAI	GCGC	2 cut(s)	1052, 1960
Kpn2I	TCCGGA	1 cut(s)	133
Ksp22I	TGATCA	2 cut(s)	1021, 1807
Kzo9I	GATC	9 cut(s)	85, 304, 611, 1021, 1258, 1487, 1807, 1991, 2050
LguI	GCTCTTC	1 cut(s)	1902
LmnI	GCTCC	5 cut(s)	193, 337, 1204, 1227, 1521
LweI	GCATC	9 cut(s)	64, 74, 1003, 1186, 1315, 1328, 1385, 1643, 2100
MabI	ACCWGGT	2 cut(s)	151, 1336
MaeII	ACGT	2 cut(s)	1424, 1923
MaeIII	GTNAC	2 cut(s)	704, 1598
MalI	GATC	9 cut(s)	87, 306, 613, 1023, 1260, 1489, 1809, 1993, 2052
MbiI	CCGCTC	2 cut(s)	271, 749
MboI	GATC	9 cut(s)	85, 304, 611, 1021, 1258, 1487, 1807, 1991, 2050
MflI	RGATCY	3 cut(s)	304, 1991, 2050
MhlI	GDGCHC	4 cut(s)	832, 1232, 1532, 2129
MroI	TCCGGA	1 cut(s)	133
MroXI	GAANNNNTTC	3 cut(s)	129, 736, 1479
MseI	TTAA	3 cut(s)	590, 1404, 1937
MslI	CAYNNNNRTG	6 cut(s)	60, 465, 756, 1129, 1423, 1656
MspA1I	CMGCKG	1 cut(s)	1415
MspI	CCGG	3 cut(s)	134, 1381, 1997
MspR9I	CCNGG	9 cut(s)	153, 221, 1338, 1359, 1381, 1518, 1526, 1604, 2027
Mva1269I	GAATGC	3 cut(s)	448, 973, 2072
MvaI	CCWGG	8 cut(s)	153, 221, 1338, 1359, 1518, 1526, 1604, 2027
NciI	CCSGG	1 cut(s)	1381
NcoI	CCATGG	1 cut(s)	55
NdeII	GATC	9 cut(s)	85, 304, 611, 1021, 1258, 1487, 1807, 1991, 2050
NlaIV	GGNNCC	6 cut(s)	28, 218, 306, 1431, 1523, 1557
NmeAIII	GCCGAG	1 cut(s)	1282
NspI	RCATGY	2 cut(s)	1861, 1882
OliI	CACNNNNGTG	1 cut(s)	1423
PaqCI	CACCTGC	3 cut(s)	752, 1304, 1694
PciI	ACATGT	1 cut(s)	1878
PciSI	GCTCTTC	1 cut(s)	1902
PcsI	WCGNNNNNNNCGW	1 cut(s)	1559
PctI	GAATGC	3 cut(s)	448, 973, 2072
PdmI	GAANNNNTTC	3 cut(s)	129, 736, 1479
PfeI	GAWTC	6 cut(s)	115, 130, 430, 854, 1785, 1801
PflMI	CCANNNNNTGG	2 cut(s)	284, 1577
PfoI	TCCNGGA	2 cut(s)	1357, 1379
Ppu21I	YACGTR	1 cut(s)	1425
PpuMI	RGGWCCY	1 cut(s)	1556
PscI	ACATGT	1 cut(s)	1878
Psp124BI	GAGCTC	1 cut(s)	1232
Psp5II	RGGWCCY	1 cut(s)	1556
Psp6I	CCWGG	8 cut(s)	151, 219, 1336, 1357, 1516, 1524, 1602, 2025
PspEI	GGTNACC	1 cut(s)	1598
PspGI	CCWGG	8 cut(s)	151, 219, 1336, 1357, 1516, 1524, 1602, 2025
PspN4I	GGNNCC	6 cut(s)	28, 218, 306, 1431, 1523, 1557
PspPI	GGNCC	2 cut(s)	1556, 1816
PspPPI	RGGWCCY	1 cut(s)	1556
PsrI	GAACNNNNNNTAC	2 cut(s)	10, 42
PstI	CTGCAG	2 cut(s)	1315, 1705
PstNI	CAGNNNCTG	3 cut(s)	433, 1283, 1415
PsuI	RGATCY	3 cut(s)	304, 1991, 2050
PvuII	CAGCTG	1 cut(s)	1415
RsaI	GTAC	5 cut(s)	19, 144, 1275, 1594, 1752
RsaNI	GTAC	5 cut(s)	18, 143, 1274, 1593, 1751
RseI	CAYNNNNRTG	6 cut(s)	60, 465, 756, 1129, 1423, 1656
SacI	GAGCTC	1 cut(s)	1232
SapI	GCTCTTC	1 cut(s)	1902
SaqAI	TTAA	3 cut(s)	590, 1404, 1937
Sau3AI	GATC	9 cut(s)	85, 304, 611, 1021, 1258, 1487, 1807, 1991, 2050
Sau96I	GGNCC	2 cut(s)	1556, 1816
SbfI	CCTGCAGG	1 cut(s)	1315
ScrFI	CCNGG	9 cut(s)	153, 221, 1338, 1359, 1381, 1518, 1526, 1604, 2027
SdaI	CCTGCAGG	1 cut(s)	1315
SduI	GDGCHC	4 cut(s)	832, 1232, 1532, 2129
SexAI	ACCWGGT	2 cut(s)	151, 1336
SfaNI	GCATC	9 cut(s)	64, 74, 1003, 1186, 1315, 1328, 1385, 1643, 2100
SfcI	CTRYAG	3 cut(s)	1311, 1701, 2142
SinI	GGWCC	2 cut(s)	1556, 1816
SmiMI	CAYNNNNRTG	6 cut(s)	60, 465, 756, 1129, 1423, 1656
SmlI	CTYRAG	5 cut(s)	205, 1036, 1150, 1217, 1231
SmoI	CTYRAG	5 cut(s)	205, 1036, 1150, 1217, 1231
Sse8387I	CCTGCAGG	1 cut(s)	1315
SsiI	CCGC	7 cut(s)	271, 455, 747, 1079, 1109, 1451, 2070
SstI	GAGCTC	1 cut(s)	1232
StyD4I	CCNGG	9 cut(s)	151, 219, 1336, 1357, 1379, 1516, 1524, 1602, 2025
StyI	CCWWGG	3 cut(s)	55, 1625, 2148
TaaI	ACNGT	2 cut(s)	681, 725
TaiI	ACGT	2 cut(s)	1427, 1926
TaqI	TCGA	5 cut(s)	535, 1462, 1486, 1788, 2064
TaqII	GACCGA	1 cut(s)	1804
TatI	WGTACW	2 cut(s)	142, 1750
TauI	GCSGC	1 cut(s)	1111
TfiI	GAWTC	6 cut(s)	115, 130, 430, 854, 1785, 1801
Tru1I	TTAA	3 cut(s)	590, 1404, 1937
Tru9I	TTAA	3 cut(s)	590, 1404, 1937
TscAI	CASTG	4 cut(s)	306, 375, 730, 1936
TspDTI	ATGAA	4 cut(s)	363, 612, 1575, 1671
TspGWI	ACGGA	4 cut(s)	127, 887, 1665, 1702
TspRI	CASTG	4 cut(s)	306, 375, 730, 1936
Van91I	CCANNNNNTGG	2 cut(s)	284, 1577
VpaK11BI	GGWCC	2 cut(s)	1556, 1816
XagI	CCTNNNNNAGG	1 cut(s)	943
XapI	RAATTY	9 cut(s)	93, 178, 531, 802, 928, 1171, 1294, 1330, 2119
XbaI	TCTAGA	1 cut(s)	82
XceI	RCATGY	2 cut(s)	1861, 1882
XcmI	CCANNNNNNNNNTGG	1 cut(s)	285
XmnI	GAANNNNTTC	3 cut(s)	129, 736, 1479

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.