Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0004946

Physical Location & Seq

Genomic Coordinates Reverse (-)

10826105 .. 10828985

Gene Structure 2881 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1118600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2082 bp

ATGGAAGAGAGTTTAGAGCCTCACTTTGTCCCCCTTATCCCATCGCTTTGTCTCTCACCCGCTCCGAGATTAGATCATATCTTCTCCTTGTGTTTTCATTCCTTACAACTCTGCAACTCCAACAACACACTCTCTCTCTCTCCCGCTGCCTGCAAATTTCTGCAATCTCAAGCTAAAAGTGCAAAGATAAACATCCATATGGGAGAATCAGGCAACAGAAATGAAGAAAAGACTGCCACTTTTGTAAATGGAACGGTCCAAGAGTTACCCATCAAGGGAAATTACCCAGATGATAAGGATCAATTGCATCTGATTTCAAAGTCTAATGATTCATTGAGACAATCCCATTGTGCTATCTGCATTGCCAAAGAGAGTTGTCGAATCGTGCGTTCGAGAACTAGGCTAATGAACATTTTGGTGGAAAGAGGGAAGCCACAGGAGGCTCAGTCCCTTTTCAATGGTTTGATTGAAGGAGGCCACATGCCATCACTGATATCATACACTACACTGTTGGCTGCCTTGACTGCCCAGAAGCGTTTCGAGTCCATTCCTTTTGTTATTTCACAGGTAGAAGAGAATGGATTGAGTCCTGATTTGATATTCTTCAATGCTGTTATAAATGCATTTTCTGAATCCGGGAACGTAGAGGAAGCAATGAAAATGTTTAGGAAGATGAAGGAGAGTGGATTGAAACCCACTACCAGTACTTATAACACATTGATCAAAGGGTACGGGATTTCGGGTAAGCCTGAGGATTCTTTGAAAGTGCTAGAGATGATGTCCCGAGAGGAAAATGTGAAGCCGAATATCAGGACTTATAATGTACTAGTCAGAGCATGGTGTAAGAAGAAGAACATCACGGAGGCATGGAGCGTGGTGCATAAGATGGTTGCTTCTGGAATGCGGCCTGATGCTGTTACTTACAACACATTAGCAACAGCTTATGCACAAAATGGAGAGACGAGTCGGGCAGAAGGACTGATTTTAGAGATGCAGAACAACAAGTTGTATCCGAACGAGCGAACTTGTGGCATTATCATAAGCGGGTATTGCAAGGAAGGTAAGTTGAAGGAGGCCTTAAGGTTTATGCGCAGGATAAAAGATCTTGGTCTGCGCCCGAACATCGTTGTCTTTAACTCGCTGGTTAAAGGTTTCGTAGACACCATGGATAGAGATGGGGTTGATCAGGTGCTGAAGTTGATGGAAGAATTTGGGGTCAAACCAGATGTGATAACATTCAGCACCATCATGAATGCATGGAGCACGGCAGGGTTTATGGAGAAGTGTAGGCAAATATTTGACGACATGGTGAAGGCCGGCATAAAACCTGATGGGCATGCATACAGCATTCTTGCCAAAGGTTACGTGCGTGCTCAAGAAGTCGAGAAAGCTGAAGAACTATTGGCAACCATGATCAAATCAGGAGTTCGTCCCAATGTTGTCATATTCACAACCATCATAAGTGGATGGTGCAGTTCTGGCAGAATGGAGAACGCGATTAAAGTTTTCGATAAGATGTACGAGTCCGGTATTTCTCCCAATTTGAAGACGTTTGAGACCCTAATATGGGGATTTGGAGAAGCAAAGCTGCCATGGAAAGCTGAAGAAATACTCCAGATTATGAAAGAGTTTGGTGTTCAGCCAGAGAAAACTACTGTCTCGCTTGTTGCAGATGCTTGGTGTTCAATTGGATTAACAAAAGAGGCAAACAGGATGCTCGGTTCTGCGAAAAGTATGGAAAAGACCCCTCAGGTTAAAAAAGGAGAGGAGCCATTTGAGAGCTTAGAGAAAATATATCAAAAGAGATCTGCAAGTGCATTTCATCCCAATGTTTTACAGATACCTTCAGCCGTAGCAAGTGATCAAAAGGGGTCAGCTTCAGCAACTAGAAAAGGAAGAATGGTTTTAAGAGATGGTGATTTCTCGTTGGACGTGTCATCGCTTGCTGCAAAGTCTATGTATCTGTCGCATAATTGTAAATTCGGGGAGCGATCGCCAATTATATGCAGGAAGCAATCTCAGGGCCAGCTTGGTGTGTATGGTCAGTTTGCACAGCAATGTACAGTTGTGTTCTTGAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

694

Amino Acids

77.15

Weight (kDa)

8.89

Isoelectric Point (pI)

43.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	161 - 209	2.1e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	193 - 226	5.4e-08	PPR repeat
PPR_2	PF13041	197 - 244	3.7e-15	PPR repeat family
PPR	PF01535	202 - 230	6.6e-08	PPR repeat
PPR_3	PF13812	206 - 245	2.8e-07	Pentatricopeptide repeat domain
PPR_long	PF17177	217 - 322	2.9e-10	Pentacotripeptide-repeat region of PRORP
TPR_24	PF23276	223 - 326	2.6e-07	Fungal tetratrico peptide repeats
PPR_1	PF12854	229 - 260	1.6e-06	PPR repeat
PPR_2	PF13041	232 - 282	1.2e-11	PPR repeat family
PPR	PF01535	235 - 263	6.4e-06	PPR repeat
PPR_1	PF12854	265 - 297	1e-06	PPR repeat
PPR_2	PF13041	268 - 316	3.8e-13	PPR repeat family
PPR_3	PF13812	291 - 350	2.9e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	303 - 352	6e-11	PPR repeat family
PPR_3	PF13812	330 - 387	7e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	336 - 365	9.8e-07	PPR repeat
PPR_2	PF13041	342 - 386	3.1e-09	PPR repeat family
PPR_long	PF17177	356 - 453	2.7e-07	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	363 - 420	1.3e-10	Pentatricopeptide repeat domain
PPR_2	PF13041	373 - 419	3e-07	PPR repeat family
PPR_2	PF13041	408 - 456	2.5e-11	PPR repeat family
PPR_long	PF17177	428 - 510	8.9e-08	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	432 - 488	2.6e-09	Pentatricopeptide repeat domain
PPR_long	PF17177	461 - 564	3.4e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	468 - 526	1.7e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	475 - 506	1.4e-11	PPR repeat
PPR_2	PF13041	478 - 525	3.3e-14	PPR repeat family
PPR	PF01535	481 - 511	2.1e-06	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0004946)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G25630 AT5G25630 AT5G25630
fragaria_vesca	FvH4_2g36130 FvH4_2g36130 FvH4_2g36130 FvH4_2g36130 FvH4_2g36130 FvH4_2g36130 FvH4_2g36130
malus_domestica	MD02G1045100.v1.1 MD08G1118600.v1.1 MD15G1097800.v1.1 MD15G1097900.v1.1
prunus_persica	Prupe.1G452700_v2.0.a1 Prupe.1G452700_v2.0.a1 Prupe.1G452700_v2.0.a1
pyrus_communis	pycom08g09890
rosa_chinensis	RchiOBHm_Chr6g0305741 RchiOBHm_Chr6g0306351
rosa_laevigata	RLG00000010863 RLG00000021840
rosa_roxburghii	Rroxscaffold_7G00162390
rosa_rugosa	Rorug06G0343700 Rorug06G0343800
rosa_samantha	Rh6AG455200 Rh6BG446100 Rh6CG469100 Rh6DG455700
rosa_wichuraiana	Rw6G039710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	617, 711, 819
Acc16I	TGCGCA	1 cut(s)	1091
AccBSI	CCGCTC	1 cut(s)	62
AccI	GTMKAC	1 cut(s)	1158
AccII	CGCG	1 cut(s)	1496
AciI	CCGC	4 cut(s)	60, 144, 904, 1044
AclWI	GGATC	1 cut(s)	306
AcsI	RAATTY	3 cut(s)	155, 1208, 1979
AcuI	CTGAAG	5 cut(s)	1214, 1413, 1623, 1830, 1863
AfaI	GTAC	5 cut(s)	706, 731, 825, 1520, 2062
AfiI	CCNNNNNNNGG	3 cut(s)	275, 1566, 1567
AflII	CTTAAG	1 cut(s)	1078
AflIII	ACRYGT	1 cut(s)	1932
AhlI	ACTAGT	1 cut(s)	826
AjiI	CACGTC	1 cut(s)	1933
AloI	GAACNNNNNNTCC	2 cut(s)	1705, 1737
AluBI	AGCT	8 cut(s)	173, 941, 1391, 1588, 1601, 1782, 1877, 2029
AluI	AGCT	8 cut(s)	173, 941, 1391, 1588, 1601, 1782, 1877, 2029
Alw21I	GWGCWC	2 cut(s)	1265, 1375
Alw26I	GTCTC	5 cut(s)	56, 331, 953, 1550, 1663
AlwI	GGATC	1 cut(s)	306
AlwNI	CAGNNNCTG	1 cut(s)	1192
Ama87I	CYCGRG	1 cut(s)	783
AoxI	GGCC	5 cut(s)	475, 905, 1074, 1314, 2023
ApeKI	GCWGC	4 cut(s)	146, 515, 1588, 1946
ApoI	RAATTY	3 cut(s)	155, 1208, 1979
AsiSI	GCGATCGC	1 cut(s)	1994
Asp700I	GAANNNNTTC	1 cut(s)	536
AspLEI	GCGC	2 cut(s)	1092, 1116
AspS9I	GGNCC	2 cut(s)	256, 2023
AsuC2I	CCSGG	1 cut(s)	637
AsuHPI	GGTGA	3 cut(s)	48, 1321, 1928
AvaI	CYCGRG	1 cut(s)	783
AvaII	GGWCC	1 cut(s)	256
AxyI	CCTNAGG	2 cut(s)	750, 1749
BbsI	GAAGAC	1 cut(s)	1553
Bbv12I	GWGCWC	2 cut(s)	1265, 1375
BbvI	GCAGC	4 cut(s)	133, 502, 1575, 1933
BceAI	ACGGC	2 cut(s)	1281, 1835
BciVI	GTATCC	1 cut(s)	1020
BclI	TGATCA	4 cut(s)	720, 1183, 1413, 1861
BcnI	CCSGG	1 cut(s)	637
BcoDI	GTCTC	5 cut(s)	56, 331, 953, 1550, 1663
BcuI	ACTAGT	1 cut(s)	826
BfaI	CTAG	4 cut(s)	399, 770, 827, 1887
BfrI	CTTAAG	1 cut(s)	1078
BfuI	GTATCC	1 cut(s)	1020
BglII	AGATCT	2 cut(s)	1102, 1805
BisI	GCNGC	5 cut(s)	147, 516, 905, 1589, 1947
BlsI	GCNGC	5 cut(s)	148, 517, 906, 1590, 1948
BmcAI	AGTACT	1 cut(s)	706
Bme1390I	CCNGG	1 cut(s)	637
Bme18I	GGWCC	1 cut(s)	256
BmeT110I	CYCGRG	1 cut(s)	783
BmgBI	CACGTC	1 cut(s)	1933
BmgT120I	GGNCC	2 cut(s)	256, 2023
BmiI	GGNNCC	1 cut(s)	1770
BmrFI	CCNGG	1 cut(s)	637
BmsI	GCATC	5 cut(s)	316, 901, 981, 1663, 1704
BpiI	GAAGAC	1 cut(s)	1553
BpmI	CTGGAG	1 cut(s)	1598
BpuEI	CTTGAG	2 cut(s)	153, 1359
BpuMI	CCSGG	1 cut(s)	637
BsaAI	YACGTR	1 cut(s)	1366
BsaBI	GATNNNNATC	2 cut(s)	191, 297
BsaI	GGTCTC	1 cut(s)	1550
BsaJI	CCNNGG	2 cut(s)	1164, 1592
BsaWI	WCCGGW	1 cut(s)	1526
BsaXI	ACNNNNNCTCC	2 cut(s)	431, 461
Bsc4I	CCNNNNNNNGG	3 cut(s)	275, 1566, 1567
Bse118I	RCCGGY	1 cut(s)	1316
Bse1I	ACTGG	1 cut(s)	702
Bse21I	CCTNAGG	2 cut(s)	750, 1749
Bse3DI	GCAATG	3 cut(s)	360, 660, 2063
Bse8I	GATNNNNATC	2 cut(s)	191, 297
BseDI	CCNNGG	2 cut(s)	1164, 1592
BseGI	GGATG	4 cut(s)	192, 1472, 1719, 1822
BseJI	GATNNNNATC	2 cut(s)	191, 297
BseLI	CCNNNNNNNGG	3 cut(s)	275, 1566, 1567
BseMI	GCAATG	3 cut(s)	360, 660, 2063
BseMII	CTCAG	4 cut(s)	458, 741, 1763, 2033
BseNI	ACTGG	1 cut(s)	702
BseRI	GAGGAG	1 cut(s)	1781
BseXI	GCAGC	4 cut(s)	133, 502, 1575, 1933
BsgI	GTGCAG	1 cut(s)	1492
Bsh1236I	CGCG	1 cut(s)	1496
Bsh1285I	CGRYCG	1 cut(s)	1994
BshFI	GGCC	5 cut(s)	477, 907, 1076, 1316, 2025
BsiEI	CGRYCG	1 cut(s)	1994
BsiHKAI	GWGCWC	2 cut(s)	1265, 1375
BsiHKCI	CYCGRG	1 cut(s)	783
BsiSI	CCGG	3 cut(s)	636, 1317, 1527
BslFI	GGGAC	4 cut(s)	14, 433, 766, 1416
BslI	CCNNNNNNNGG	3 cut(s)	275, 1566, 1567
BsmAI	GTCTC	5 cut(s)	56, 331, 953, 1550, 1663
BsmBI	CGTCTC	1 cut(s)	953
BsmFI	GGGAC	4 cut(s)	14, 433, 766, 1416
BsmI	GAATGC	3 cut(s)	906, 1258, 1347
BsnI	GGCC	5 cut(s)	477, 907, 1076, 1316, 2025
Bso31I	GGTCTC	1 cut(s)	1550
BsoBI	CYCGRG	1 cut(s)	783
Bsp1286I	GDGCHC	2 cut(s)	1265, 1375
Bsp1407I	TGTACA	1 cut(s)	2060
Bsp143I	GATC	9 cut(s)	73, 298, 720, 1102, 1183, 1413, 1805, 1861, 1991
Bsp19I	CCATGG	2 cut(s)	1164, 1592
BspACI	CCGC	4 cut(s)	60, 144, 904, 1044
BspANI	GGCC	5 cut(s)	477, 907, 1076, 1316, 2025
BspCNI	CTCAG	4 cut(s)	457, 742, 1762, 2032
BspFNI	CGCG	1 cut(s)	1496
BspHI	TCATGA	1 cut(s)	1248
BspLI	GGNNCC	1 cut(s)	1770
BspPI	GGATC	1 cut(s)	306
BspTI	CTTAAG	1 cut(s)	1078
BspTNI	GGTCTC	1 cut(s)	1550
BsrBI	CCGCTC	1 cut(s)	62
BsrDI	GCAATG	3 cut(s)	360, 660, 2063
BsrFI	RCCGGY	1 cut(s)	1316
BsrGI	TGTACA	1 cut(s)	2060
BsrI	ACTGG	1 cut(s)	702
BssAI	RCCGGY	1 cut(s)	1316
BssECI	CCNNGG	2 cut(s)	1164, 1592
BssMI	GATC	9 cut(s)	73, 298, 720, 1102, 1183, 1413, 1805, 1861, 1991
BssT1I	CCWWGG	2 cut(s)	1164, 1592
Bst4CI	ACNGT	4 cut(s)	256, 510, 1657, 2065
Bst6I	CTCTTC	1 cut(s)	567
BstAFI	CTTAAG	1 cut(s)	1078
BstAUI	TGTACA	1 cut(s)	2060
BstBAI	YACGTR	1 cut(s)	1366
BstC8I	GCNNGC	6 cut(s)	151, 1318, 1338, 1371, 1944, 2027
BstDEI	CTNAG	5 cut(s)	444, 750, 1749, 1783, 2019
BstDSI	CCRYGG	2 cut(s)	1164, 1592
BstF5I	GGATG	4 cut(s)	192, 1472, 1719, 1822
BstFNI	CGCG	1 cut(s)	1496
BstHHI	GCGC	2 cut(s)	1092, 1116
BstKTI	GATC	9 cut(s)	76, 301, 723, 1105, 1186, 1416, 1808, 1864, 1994
BstMAI	GTCTC	5 cut(s)	56, 331, 953, 1550, 1663
BstMBI	GATC	9 cut(s)	73, 298, 720, 1102, 1183, 1413, 1805, 1861, 1991
BstMCI	CGRYCG	1 cut(s)	1994
BstMWI	GCNNNNNNNGC	4 cut(s)	179, 524, 1050, 1479
BstNSI	RCATGY	2 cut(s)	484, 1340
BstSCI	CCNGG	1 cut(s)	635
BstUI	CGCG	1 cut(s)	1496
BstV1I	GCAGC	4 cut(s)	133, 502, 1575, 1933
BstV2I	GAAGAC	1 cut(s)	1553
BstX2I	RGATCY	2 cut(s)	1102, 1805
BstYI	RGATCY	2 cut(s)	1102, 1805
Bsu36I	CCTNAGG	2 cut(s)	750, 1749
BsuI	GTATCC	1 cut(s)	1020
BsuRI	GGCC	5 cut(s)	477, 907, 1076, 1316, 2025
BtgI	CCRYGG	2 cut(s)	1164, 1592
BtgZI	GCGATG	2 cut(s)	27, 1923
BtrI	CACGTC	1 cut(s)	1933
BtsCI	GGATG	4 cut(s)	192, 1472, 1719, 1822
BtsIMutI	CAGTG	2 cut(s)	488, 506
Cac8I	GCNNGC	6 cut(s)	151, 1318, 1338, 1371, 1944, 2027
CaiI	CAGNNNCTG	1 cut(s)	1192
CciI	TCATGA	1 cut(s)	1248
CfoI	GCGC	2 cut(s)	1092, 1116
Cfr10I	RCCGGY	1 cut(s)	1316
Cfr13I	GGNCC	2 cut(s)	256, 2023
Csp6I	GTAC	5 cut(s)	705, 730, 824, 1519, 2061
CviQI	GTAC	5 cut(s)	705, 730, 824, 1519, 2061
DdeI	CTNAG	5 cut(s)	444, 750, 1749, 1783, 2019
DpnI	GATC	9 cut(s)	75, 300, 722, 1104, 1185, 1415, 1807, 1863, 1993
DpnII	GATC	9 cut(s)	73, 298, 720, 1102, 1183, 1413, 1805, 1861, 1991
Eam1104I	CTCTTC	1 cut(s)	567
EarI	CTCTTC	1 cut(s)	567
Eco130I	CCWWGG	2 cut(s)	1164, 1592
Eco147I	AGGCCT	1 cut(s)	1076
Eco31I	GGTCTC	1 cut(s)	1550
Eco32I	GATATC	1 cut(s)	495
Eco47I	GGWCC	1 cut(s)	256
Eco57I	CTGAAG	5 cut(s)	1214, 1413, 1623, 1830, 1863
Eco81I	CCTNAGG	2 cut(s)	750, 1749
Eco88I	CYCGRG	1 cut(s)	783
EcoRV	GATATC	1 cut(s)	495
EcoT14I	CCWWGG	2 cut(s)	1164, 1592
EcoT22I	ATGCAT	3 cut(s)	625, 1258, 1342
ErhI	CCWWGG	2 cut(s)	1164, 1592
Esp3I	CGTCTC	1 cut(s)	953
FalI	AAGNNNNNCTT	2 cut(s)	1061, 1093
FaqI	GGGAC	4 cut(s)	14, 433, 766, 1416
FauI	CCCGC	3 cut(s)	67, 151, 1037
FauNDI	CATATG	1 cut(s)	198
FbaI	TGATCA	4 cut(s)	720, 1183, 1413, 1861
FblI	GTMKAC	1 cut(s)	1158
Fnu4HI	GCNGC	5 cut(s)	147, 516, 905, 1589, 1947
FokI	GGATG	4 cut(s)	179, 1479, 1726, 1809
Fsp4HI	GCNGC	5 cut(s)	147, 516, 905, 1589, 1947
FspBI	CTAG	4 cut(s)	399, 770, 827, 1887
FspI	TGCGCA	1 cut(s)	1091
GlaI	GCGC	2 cut(s)	1091, 1115
GluI	GCNGC	5 cut(s)	147, 516, 905, 1589, 1947
GsuI	CTGGAG	1 cut(s)	1598
HaeIII	GGCC	5 cut(s)	477, 907, 1076, 1316, 2025
HapII	CCGG	3 cut(s)	636, 1317, 1527
HhaI	GCGC	2 cut(s)	1092, 1116
Hin6I	GCGC	2 cut(s)	1090, 1114
HinP1I	GCGC	2 cut(s)	1090, 1114
HinfI	GANTC	9 cut(s)	206, 329, 381, 542, 586, 632, 755, 964, 1523
HpaII	CCGG	3 cut(s)	636, 1317, 1527
HphI	GGTGA	3 cut(s)	48, 1321, 1928
Hpy166II	GTNNAC	1 cut(s)	1159
Hpy188I	TCNGA	5 cut(s)	66, 312, 631, 833, 1014
Hpy8I	GTNNAC	1 cut(s)	1159
HpyAV	CCTTC	7 cut(s)	464, 670, 968, 1052, 1063, 1306, 1854
HpyCH4III	ACNGT	4 cut(s)	256, 510, 1657, 2065
HpyCH4IV	ACGT	4 cut(s)	642, 1365, 1550, 1932
HpyF10VI	GCNNNNNNNGC	4 cut(s)	179, 524, 1050, 1479
HpyF3I	CTNAG	5 cut(s)	444, 750, 1749, 1783, 2019
HpySE526I	ACGT	4 cut(s)	642, 1365, 1550, 1932
HspAI	GCGC	2 cut(s)	1090, 1114
KroI	GCCGGC	1 cut(s)	1316
KroNI	GCCGGC	1 cut(s)	1318
Ksp22I	TGATCA	4 cut(s)	720, 1183, 1413, 1861
Kzo9I	GATC	9 cut(s)	73, 298, 720, 1102, 1183, 1413, 1805, 1861, 1991
LmnI	GCTCC	5 cut(s)	67, 870, 1260, 1768, 1987
Lsp1109I	GCAGC	4 cut(s)	133, 502, 1575, 1933
LweI	GCATC	5 cut(s)	316, 901, 981, 1663, 1704
MaeI	CTAG	4 cut(s)	399, 770, 827, 1887
MaeII	ACGT	4 cut(s)	642, 1365, 1550, 1932
MaeIII	GTNAC	3 cut(s)	264, 916, 1361
MalI	GATC	9 cut(s)	75, 300, 722, 1104, 1185, 1415, 1807, 1863, 1993
MbiI	CCGCTC	1 cut(s)	62
MboI	GATC	9 cut(s)	73, 298, 720, 1102, 1183, 1413, 1805, 1861, 1991
MfeI	CAATTG	2 cut(s)	302, 1686
MflI	RGATCY	2 cut(s)	1102, 1805
MhlI	GDGCHC	2 cut(s)	1265, 1375
MluCI	AATT	9 cut(s)	155, 280, 302, 1208, 1540, 1686, 1972, 1979, 1998
MlyI	GAGTC	4 cut(s)	551, 595, 973, 1532
MmeI	TCCRAC	2 cut(s)	144, 1908
Mph1103I	ATGCAT	3 cut(s)	625, 1258, 1342
MroNI	GCCGGC	1 cut(s)	1316
MroXI	GAANNNNTTC	1 cut(s)	536
MseI	TTAA	7 cut(s)	1079, 1134, 1146, 1500, 1694, 1755, 1907
MslI	CAYNNNNRTG	5 cut(s)	197, 416, 1247, 1827, 2056
MspA1I	CMGCKG	1 cut(s)	146
MspCI	CTTAAG	1 cut(s)	1078
MspI	CCGG	3 cut(s)	636, 1317, 1527
MspR9I	CCNGG	1 cut(s)	637
MunI	CAATTG	2 cut(s)	302, 1686
Mva1269I	GAATGC	3 cut(s)	906, 1258, 1347
MvnI	CGCG	1 cut(s)	1496
MwoI	GCNNNNNNNGC	4 cut(s)	179, 524, 1050, 1479
NaeI	GCCGGC	1 cut(s)	1318
NciI	CCSGG	1 cut(s)	637
NcoI	CCATGG	2 cut(s)	1164, 1592
NdeI	CATATG	1 cut(s)	198
NdeII	GATC	9 cut(s)	73, 298, 720, 1102, 1183, 1413, 1805, 1861, 1991
NgoMIV	GCCGGC	1 cut(s)	1316
NlaIV	GGNNCC	1 cut(s)	1770
NsbI	TGCGCA	1 cut(s)	1091
NsiI	ATGCAT	3 cut(s)	625, 1258, 1342
NspI	RCATGY	2 cut(s)	484, 1340
PaeI	GCATGC	1 cut(s)	1340
PagI	TCATGA	1 cut(s)	1248
PceI	AGGCCT	1 cut(s)	1076
PcsI	WCGNNNNNNNCGW	1 cut(s)	385
PctI	GAATGC	3 cut(s)	906, 1258, 1347
PdiI	GCCGGC	1 cut(s)	1318
PdmI	GAANNNNTTC	1 cut(s)	536
PfeI	GAWTC	5 cut(s)	206, 329, 381, 632, 755
PfoI	TCCNGGA	1 cut(s)	635
PkrI	GCNGC	5 cut(s)	148, 517, 906, 1590, 1948
Ple19I	CGATCG	1 cut(s)	1994
PleI	GAGTC	4 cut(s)	550, 594, 972, 1531
PpsI	GAGTC	4 cut(s)	550, 594, 972, 1531
Ppu21I	YACGTR	1 cut(s)	1366
PsiI	TTATAA	3 cut(s)	617, 711, 819
PspN4I	GGNNCC	1 cut(s)	1770
PspPI	GGNCC	2 cut(s)	256, 2023
PstNI	CAGNNNCTG	1 cut(s)	1192
PsuI	RGATCY	2 cut(s)	1102, 1805
PvuI	CGATCG	1 cut(s)	1994
RgaI	GCGATCGC	1 cut(s)	1994
RsaI	GTAC	5 cut(s)	706, 731, 825, 1520, 2062
RsaNI	GTAC	5 cut(s)	705, 730, 824, 1519, 2061
RseI	CAYNNNNRTG	5 cut(s)	197, 416, 1247, 1827, 2056
SaqAI	TTAA	7 cut(s)	1079, 1134, 1146, 1500, 1694, 1755, 1907
SatI	GCNGC	5 cut(s)	147, 516, 905, 1589, 1947
Sau3AI	GATC	9 cut(s)	73, 298, 720, 1102, 1183, 1413, 1805, 1861, 1991
Sau96I	GGNCC	2 cut(s)	256, 2023
ScaI	AGTACT	1 cut(s)	706
SchI	GAGTC	4 cut(s)	551, 595, 973, 1532
ScrFI	CCNGG	1 cut(s)	637
SduI	GDGCHC	2 cut(s)	1265, 1375
SfaAI	GCGATCGC	1 cut(s)	1994
SfaNI	GCATC	5 cut(s)	316, 901, 981, 1663, 1704
SgfI	GCGATCGC	1 cut(s)	1994
SinI	GGWCC	1 cut(s)	256
SmiMI	CAYNNNNRTG	5 cut(s)	197, 416, 1247, 1827, 2056
SmlI	CTYRAG	3 cut(s)	168, 1078, 1374
SmoI	CTYRAG	3 cut(s)	168, 1078, 1374
SpeI	ACTAGT	1 cut(s)	826
SphI	GCATGC	1 cut(s)	1340
Sse9I	AATT	9 cut(s)	155, 280, 302, 1208, 1540, 1686, 1972, 1979, 1998
SseBI	AGGCCT	1 cut(s)	1076
SsiI	CCGC	4 cut(s)	60, 144, 904, 1044
SspI	AATATT	1 cut(s)	1296
SspMI	CTAG	4 cut(s)	399, 770, 827, 1887
StuI	AGGCCT	1 cut(s)	1076
StyD4I	CCNGG	1 cut(s)	635
StyI	CCWWGG	2 cut(s)	1164, 1592
TaaI	ACNGT	4 cut(s)	256, 510, 1657, 2065
TaiI	ACGT	4 cut(s)	645, 1368, 1553, 1935
TaqI	TCGA	5 cut(s)	379, 392, 540, 1383, 1509
TasI	AATT	9 cut(s)	155, 280, 302, 1208, 1540, 1686, 1972, 1979, 1998
TatI	WGTACW	3 cut(s)	704, 823, 2060
TauI	GCSGC	1 cut(s)	907
TfiI	GAWTC	5 cut(s)	206, 329, 381, 632, 755
Tru1I	TTAA	7 cut(s)	1079, 1134, 1146, 1500, 1694, 1755, 1907
Tru9I	TTAA	7 cut(s)	1079, 1134, 1146, 1500, 1694, 1755, 1907
TscAI	CASTG	2 cut(s)	495, 513
TseI	GCWGC	4 cut(s)	146, 515, 1588, 1946
TspDTI	ATGAA	9 cut(s)	86, 237, 321, 422, 671, 689, 1265, 1637, 1811
TspGWI	ACGGA	1 cut(s)	875
TspRI	CASTG	2 cut(s)	495, 513
Vha464I	CTTAAG	1 cut(s)	1078
VpaK11BI	GGWCC	1 cut(s)	256
XapI	RAATTY	3 cut(s)	155, 1208, 1979
XceI	RCATGY	2 cut(s)	484, 1340
XmiI	GTMKAC	1 cut(s)	1158
XmnI	GAANNNNTTC	1 cut(s)	536
XspI	CTAG	4 cut(s)	399, 770, 827, 1887
ZrmI	AGTACT	1 cut(s)	706
Zsp2I	ATGCAT	3 cut(s)	625, 1258, 1342

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.