Basic Information

Type: gene

Biological Identity

Species

prunus_persica

Chromosome

Pp01

Orthogroup ID

OG0004946

Physical Location & Seq

Genomic Coordinates Reverse (-)

38073410 .. 38076454

Gene Structure 3045 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Prupe.1G452700.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1911 bp

ATGGGAGAATCAGGCAAAGAGAAGGCTTTTTTAAGAACAACCAACAAAAACGAAGAAGCAACTGCCACTACTGCAAATGGACGGGTTCAAGCATTACCCATCAAGGGAAATCATCCAGATGATAAGAACCATTTGCGTCTAATTTCAAAACCTAATGGCTCAATGAGGCAATCCCATTGTTCTATCTGCATGGCCAAAGAGAATTGTCGAATTGTGCGATCGAGAACTAGGCTAATGAACATTTTGATAGAGAGAGGGAGACCTCAGGAAGCCCAGTCCCTTTTTAATGGTTTAATTGAAGGAGGACACAGGCCTTCACTGGTATCGTACACAACCCTCCTAGCTGCCTTGACCATCCAGAAGCGCTTTGAGTCCATTCCTTATGTTATATCACAGGTGGAAGAGAATGGAATGAATCCTGATTTAATATTTTTCAATGCTGTTATAAATGCTTTTTCGGAATCTGGGAATGTAGAGAAAGCCATGGAAACCTTAAGGAAGATGAAGGAGAGTGGATTAAAACCCACTACCAGCACTTATAACACATTGATCAAAGGGTATGGGATTTCTGGTAAGCCTGAAGATTCTTTGCAACTGCTCGAGATGATGTCCCAAGAGGAAAATGTGAAACCAAATATAAGGACTTTTAATGTTCTAGTCAGAGCATGGTGTAAGAAGAACATTACAGAGGCATGGAATGTGGTGCATAAGATGGTAACTTCTGATATGCGACCTGATGCCGTTACCTACAACACATTAGCAACAGCATATGCTCAAAATGGAGAGACAAGTAAGGCAGAAGGAATGATTCTAGAGATGCAGAACAACAAAGTGCAACCCAATGAACGAACTTGTGGCATTATCATAAGTGGGTATTGTAAGGAAGGTAAACTGAAGGAGGCCTTAAGGTTTATGAAAAGGATAAAAGATCTTGGGCTGCATCCAAACATCGTTGTCTTTAACTCACTGGTTAAAGGTTTTGTTGATACCATGGATAGAGATGGGGTCGATCAGGTGCTGAAGTTGATGGAAGAATTTGGGGTAAAACCAGATGTGATAACATTTAGCACCATCATGAATGCATGGAGCACAGCCGGATTCATGGAGAAGTGTAGGCAAATCTTTGATGATATGGTGAAGGCTGGCATAAAACCTGACACGCATGCATACAGCATTCTTGCCAAAGGTTATGTGCGTGCTCAAGAAGTGGAGAAAGCTGAAGAACTGTTAGCAGCCATGATGAAATCGGGAGTTCGTCCAAATGTTGTGATTTTCACAACCATCATGAGTGGATGGTGCAGTTCTGGCAGAATGGAGCATGCAATTAGAGTTTTTGATAAGATGTGTGAACATGGAATTTCTCCCAATTTGAAGACATTTGAGACCCTAATATGGGGATTTGGAGAAACAAAGCTGCCATGGAAAGCTGAAGAAATACTCCAGATTATGAAAGAGTTTGGTGTTCAGCCAGAAAAATCTACTATCTCGCTTGTTGCTGATGCTTGGCGTGCGATTGGATTAACAAAGGAGGCAAATAGGATGCTGGGTGCTATGAGAAGTAAAGAGAAGACCAATCAGGTTGAAACCGAAGAGGTACCATTTGAGAGCTTAGAGAAAATTTATCATAAGAAATCTGCAAGTGCCTTTCATCCGAATCTGTTACAGATACCATCAGTAGTTACAAGTGATCAAAAAGGATCAGCAGTTTCAGCAACTAGAAAAGGAAGACTGGTGCTAAGAGACGGTGATTTTTCATTAGACATCTCATCGCTTGCTGCAAAATCTATGAATCTGTCTCAGACTTGTAAATTTGGGGAAAGATTGCCAATTATATGCCGGAAGCAATCTCAGGGGCAGCTTGGTGTGTATGGTCAGTTTGCACAGCAATGTACAGTTGTGTTCTTGAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

637

Amino Acids

71.13

Weight (kDa)

9.07

Isoelectric Point (pI)

34.07

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004946)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G25630 AT5G25630 AT5G25630
fragaria_vesca	FvH4_2g36130 FvH4_2g36130 FvH4_2g36130 FvH4_2g36130 FvH4_2g36130 FvH4_2g36130 FvH4_2g36130
malus_domestica	MD02G1045100.v1.1 MD08G1118600.v1.1 MD15G1097800.v1.1 MD15G1097900.v1.1
prunus_persica	Prupe.1G452700_v2.0.a1 Prupe.1G452700_v2.0.a1 Prupe.1G452700_v2.0.a1
pyrus_communis	pycom08g09890
rosa_chinensis	RchiOBHm_Chr6g0305741 RchiOBHm_Chr6g0306351
rosa_laevigata	RLG00000010863 RLG00000021840
rosa_roxburghii	Rroxscaffold_7G00162390
rosa_rugosa	Rorug06G0343700 Rorug06G0343800
rosa_samantha	Rh6AG455200 Rh6BG446100 Rh6CG469100 Rh6DG455700
rosa_wichuraiana	Rw6G039710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	446, 540
Acc65I	GGTACC	1 cut(s)	1594
AccB1I	GGYRCC	1 cut(s)	1594
AclWI	GGATC	1 cut(s)	1705
AcoI	YGGCCR	1 cut(s)	192
AcsI	RAATTY	4 cut(s)	1034, 1356, 1617, 1808
AcuI	CTGAAG	5 cut(s)	600, 914, 1040, 1239, 1449
AfaI	GTAC	3 cut(s)	329, 1596, 1891
AfeI	AGCGCT	1 cut(s)	365
AfiI	CCNNNNNNNGG	3 cut(s)	104, 1392, 1393
AflII	CTTAAG	2 cut(s)	493, 904
AgsI	TTSAA	7 cut(s)	89, 147, 299, 436, 1372, 1583, 1906
AluBI	AGCT	6 cut(s)	344, 1217, 1414, 1427, 1608, 1858
AluI	AGCT	6 cut(s)	344, 1217, 1414, 1427, 1608, 1858
Alw21I	GWGCWC	2 cut(s)	1091, 1201
Alw26I	GTCTC	5 cut(s)	253, 779, 1376, 1734, 1800
AlwI	GGATC	1 cut(s)	1705
AlwNI	CAGNNNCTG	1 cut(s)	1018
Ama87I	CYCGRG	1 cut(s)	599
Aor51HI	AGCGCT	1 cut(s)	365
AoxI	GGCC	3 cut(s)	192, 311, 900
ApeKI	GCWGC	6 cut(s)	344, 937, 1232, 1414, 1775, 1855
ApoI	RAATTY	4 cut(s)	1034, 1356, 1617, 1808
Asp718I	GGTACC	1 cut(s)	1594
AspLEI	GCGC	1 cut(s)	366
AsuHPI	GGTGA	2 cut(s)	1147, 1757
AvaI	CYCGRG	1 cut(s)	599
AxyI	CCTNAGG	1 cut(s)	264
BalI	TGGCCA	1 cut(s)	194
BanI	GGYRCC	1 cut(s)	1594
BbsI	GAAGAC	3 cut(s)	1379, 1574, 1732
Bbv12I	GWGCWC	2 cut(s)	1091, 1201
BbvI	GCAGC	6 cut(s)	331, 924, 1244, 1401, 1762, 1867
BccI	CCATC	9 cut(s)	107, 362, 706, 995, 1021, 1079, 1287, 1289, 1678
BceAI	ACGGC	1 cut(s)	725
BclI	TGATCA	2 cut(s)	549, 1687
BcoDI	GTCTC	5 cut(s)	253, 779, 1376, 1734, 1800
BfaI	CTAG	5 cut(s)	228, 341, 656, 812, 1716
BfoI	RGCGCY	1 cut(s)	367
BfrI	CTTAAG	2 cut(s)	493, 904
BglII	AGATCT	1 cut(s)	928
BisI	GCNGC	6 cut(s)	345, 938, 1233, 1415, 1776, 1856
BlsI	GCNGC	6 cut(s)	346, 939, 1234, 1416, 1777, 1857
BmeT110I	CYCGRG	1 cut(s)	599
BmiI	GGNNCC	1 cut(s)	1596
BmrI	ACTGGG	1 cut(s)	268
BmsI	GCATC	5 cut(s)	727, 807, 949, 1489, 1530
BmuI	ACTGGG	1 cut(s)	268
BpiI	GAAGAC	3 cut(s)	1379, 1574, 1732
BpmI	CTGGAG	1 cut(s)	1424
BpuEI	CTTGAG	1 cut(s)	1185
BsaI	GGTCTC	2 cut(s)	253, 1376
BsaJI	CCNNGG	3 cut(s)	483, 990, 1418
Bsc4I	CCNNNNNNNGG	3 cut(s)	104, 1392, 1393
Bse1I	ACTGG	4 cut(s)	274, 324, 972, 1734
Bse21I	CCTNAGG	1 cut(s)	264
Bse3DI	GCAATG	1 cut(s)	1892
BseDI	CCNNGG	3 cut(s)	483, 990, 1418
BseGI	GGATG	6 cut(s)	112, 354, 940, 1298, 1545, 1648
BseLI	CCNNNNNNNGG	3 cut(s)	104, 1392, 1393
BseMI	GCAATG	1 cut(s)	1892
BseMII	CTCAG	3 cut(s)	278, 1811, 1862
BseNI	ACTGG	4 cut(s)	274, 324, 972, 1734
BseXI	GCAGC	6 cut(s)	331, 924, 1244, 1401, 1762, 1867
BseYI	CCCAGC	1 cut(s)	1543
BsgI	GTGCAG	1 cut(s)	1318
Bsh1285I	CGRYCG	1 cut(s)	221
BshFI	GGCC	3 cut(s)	194, 313, 902
BshNI	GGYRCC	1 cut(s)	1594
BsiEI	CGRYCG	1 cut(s)	221
BsiHKAI	GWGCWC	2 cut(s)	1091, 1201
BsiHKCI	CYCGRG	1 cut(s)	599
BsiSI	CCGG	2 cut(s)	1095, 1837
BslFI	GGGAC	2 cut(s)	262, 595
BslI	CCNNNNNNNGG	3 cut(s)	104, 1392, 1393
BsmAI	GTCTC	5 cut(s)	253, 779, 1376, 1734, 1800
BsmBI	CGTCTC	1 cut(s)	1734
BsmFI	GGGAC	2 cut(s)	262, 595
BsmI	GAATGC	2 cut(s)	1084, 1173
BsnI	GGCC	3 cut(s)	194, 313, 902
Bso31I	GGTCTC	2 cut(s)	253, 1376
BsoBI	CYCGRG	1 cut(s)	599
Bsp1286I	GDGCHC	2 cut(s)	1091, 1201
Bsp1407I	TGTACA	1 cut(s)	1889
Bsp143I	GATC	6 cut(s)	218, 549, 928, 1009, 1687, 1697
Bsp19I	CCATGG	3 cut(s)	483, 990, 1418
BspANI	GGCC	3 cut(s)	194, 313, 902
BspCNI	CTCAG	3 cut(s)	277, 1810, 1861
BspHI	TCATGA	2 cut(s)	1074, 1284
BspLI	GGNNCC	1 cut(s)	1596
BspPI	GGATC	1 cut(s)	1705
BspT107I	GGYRCC	1 cut(s)	1594
BspTI	CTTAAG	2 cut(s)	493, 904
BspTNI	GGTCTC	2 cut(s)	253, 1376
BsrDI	GCAATG	1 cut(s)	1892
BsrGI	TGTACA	1 cut(s)	1889
BsrI	ACTGG	4 cut(s)	274, 324, 972, 1734
BssECI	CCNNGG	3 cut(s)	483, 990, 1418
BssMI	GATC	6 cut(s)	218, 549, 928, 1009, 1687, 1697
BssT1I	CCWWGG	3 cut(s)	483, 990, 1418
Bst4CI	ACNGT	3 cut(s)	1227, 1745, 1894
Bst6I	CTCTTC	2 cut(s)	396, 1584
BstAFI	CTTAAG	2 cut(s)	493, 904
BstAUI	TGTACA	1 cut(s)	1889
BstC8I	GCNNGC	6 cut(s)	1144, 1164, 1197, 1320, 1509, 1773
BstDEI	CTNAG	5 cut(s)	264, 1609, 1736, 1797, 1848
BstDSI	CCRYGG	3 cut(s)	483, 990, 1418
BstF5I	GGATG	6 cut(s)	112, 354, 940, 1298, 1545, 1648
BstH2I	RGCGCY	1 cut(s)	367
BstHHI	GCGC	1 cut(s)	366
BstKTI	GATC	6 cut(s)	221, 552, 931, 1012, 1690, 1700
BstMAI	GTCTC	5 cut(s)	253, 779, 1376, 1734, 1800
BstMBI	GATC	6 cut(s)	218, 549, 928, 1009, 1687, 1697
BstMCI	CGRYCG	1 cut(s)	221
BstMWI	GCNNNNNNNGC	4 cut(s)	71, 1305, 1508, 1709
BstNSI	RCATGY	2 cut(s)	1166, 1322
BstV1I	GCAGC	6 cut(s)	331, 924, 1244, 1401, 1762, 1867
BstV2I	GAAGAC	3 cut(s)	1379, 1574, 1732
BstX2I	RGATCY	1 cut(s)	928
BstYI	RGATCY	1 cut(s)	928
Bsu36I	CCTNAGG	1 cut(s)	264
BsuRI	GGCC	3 cut(s)	194, 313, 902
BtgI	CCRYGG	3 cut(s)	483, 990, 1418
BtgZI	GCGATG	1 cut(s)	1752
BtsCI	GGATG	6 cut(s)	112, 354, 940, 1298, 1545, 1648
BtsIMutI	CAGTG	2 cut(s)	317, 965
Cac8I	GCNNGC	6 cut(s)	1144, 1164, 1197, 1320, 1509, 1773
CaiI	CAGNNNCTG	1 cut(s)	1018
CciI	TCATGA	2 cut(s)	1074, 1284
CfoI	GCGC	1 cut(s)	366
CseI	GACGC	1 cut(s)	125
Csp6I	GTAC	3 cut(s)	328, 1595, 1890
CviQI	GTAC	3 cut(s)	328, 1595, 1890
DdeI	CTNAG	5 cut(s)	264, 1609, 1736, 1797, 1848
DpnI	GATC	6 cut(s)	220, 551, 930, 1011, 1689, 1699
DpnII	GATC	6 cut(s)	218, 549, 928, 1009, 1687, 1697
EaeI	YGGCCR	1 cut(s)	192
Eam1104I	CTCTTC	2 cut(s)	396, 1584
EarI	CTCTTC	2 cut(s)	396, 1584
Eco130I	CCWWGG	3 cut(s)	483, 990, 1418
Eco147I	AGGCCT	2 cut(s)	313, 902
Eco31I	GGTCTC	2 cut(s)	253, 1376
Eco47III	AGCGCT	1 cut(s)	365
Eco57I	CTGAAG	5 cut(s)	600, 914, 1040, 1239, 1449
Eco81I	CCTNAGG	1 cut(s)	264
Eco88I	CYCGRG	1 cut(s)	599
EcoT14I	CCWWGG	3 cut(s)	483, 990, 1418
EcoT22I	ATGCAT	2 cut(s)	1084, 1168
ErhI	CCWWGG	3 cut(s)	483, 990, 1418
Esp3I	CGTCTC	1 cut(s)	1734
FalI	AAGNNNNNCTT	2 cut(s)	887, 919
FaqI	GGGAC	2 cut(s)	262, 595
FauNDI	CATATG	1 cut(s)	769
FbaI	TGATCA	2 cut(s)	549, 1687
Fnu4HI	GCNGC	6 cut(s)	345, 938, 1233, 1415, 1776, 1856
FokI	GGATG	6 cut(s)	99, 341, 927, 1305, 1552, 1635
Fsp4HI	GCNGC	6 cut(s)	345, 938, 1233, 1415, 1776, 1856
FspBI	CTAG	5 cut(s)	228, 341, 656, 812, 1716
GlaI	GCGC	1 cut(s)	365
GluI	GCNGC	6 cut(s)	345, 938, 1233, 1415, 1776, 1856
GsaI	CCCAGC	1 cut(s)	1547
GsuI	CTGGAG	1 cut(s)	1424
HaeII	RGCGCY	1 cut(s)	367
HaeIII	GGCC	3 cut(s)	194, 313, 902
HapII	CCGG	2 cut(s)	1095, 1837
HgaI	GACGC	1 cut(s)	125
HhaI	GCGC	1 cut(s)	366
Hin6I	GCGC	1 cut(s)	364
HinP1I	GCGC	1 cut(s)	364
HinfI	GANTC	9 cut(s)	8, 371, 415, 461, 584, 808, 1098, 1654, 1789
HpaII	CCGG	2 cut(s)	1095, 1837
HphI	GGTGA	2 cut(s)	1147, 1757
Hpy166II	GTNNAC	3 cut(s)	330, 890, 1349
Hpy188I	TCNGA	5 cut(s)	460, 662, 724, 1653, 1800
Hpy8I	GTNNAC	3 cut(s)	330, 890, 1349
HpyAV	CCTTC	8 cut(s)	16, 293, 324, 499, 794, 878, 889, 1132
HpyCH4III	ACNGT	3 cut(s)	1227, 1745, 1894
HpyF10VI	GCNNNNNNNGC	4 cut(s)	71, 1305, 1508, 1709
HpyF3I	CTNAG	5 cut(s)	264, 1609, 1736, 1797, 1848
HspAI	GCGC	1 cut(s)	364
KpnI	GGTACC	1 cut(s)	1598
Ksp22I	TGATCA	2 cut(s)	549, 1687
Kzo9I	GATC	6 cut(s)	218, 549, 928, 1009, 1687, 1697
LmnI	GCTCC	2 cut(s)	1086, 1315
Lsp1109I	GCAGC	6 cut(s)	331, 924, 1244, 1401, 1762, 1867
LweI	GCATC	5 cut(s)	727, 807, 949, 1489, 1530
MaeI	CTAG	5 cut(s)	228, 341, 656, 812, 1716
MaeIII	GTNAC	4 cut(s)	715, 742, 1659, 1678
MalI	GATC	6 cut(s)	220, 551, 930, 1011, 1689, 1699
MboI	GATC	6 cut(s)	218, 549, 928, 1009, 1687, 1697
MflI	RGATCY	1 cut(s)	928
MhlI	GDGCHC	2 cut(s)	1091, 1201
MlsI	TGGCCA	1 cut(s)	194
MluNI	TGGCCA	1 cut(s)	194
MlyI	GAGTC	1 cut(s)	380
Mox20I	TGGCCA	1 cut(s)	194
Mph1103I	ATGCAT	2 cut(s)	1084, 1168
MscI	TGGCCA	1 cut(s)	194
MslI	CAYNNNNRTG	3 cut(s)	117, 1073, 1885
Msp20I	TGGCCA	1 cut(s)	194
MspCI	CTTAAG	2 cut(s)	493, 904
MspI	CCGG	2 cut(s)	1095, 1837
Mva1269I	GAATGC	2 cut(s)	1084, 1173
MwoI	GCNNNNNNNGC	4 cut(s)	71, 1305, 1508, 1709
NcoI	CCATGG	3 cut(s)	483, 990, 1418
NdeI	CATATG	1 cut(s)	769
NdeII	GATC	6 cut(s)	218, 549, 928, 1009, 1687, 1697
NlaIV	GGNNCC	1 cut(s)	1596
NsiI	ATGCAT	2 cut(s)	1084, 1168
NspI	RCATGY	2 cut(s)	1166, 1322
PaeI	GCATGC	2 cut(s)	1166, 1322
PaeR7I	CTCGAG	1 cut(s)	599
PagI	TCATGA	2 cut(s)	1074, 1284
PceI	AGGCCT	2 cut(s)	313, 902
PcsI	WCGNNNNNNNCGW	1 cut(s)	214
PctI	GAATGC	2 cut(s)	1084, 1173
PfeI	GAWTC	8 cut(s)	8, 415, 461, 584, 808, 1098, 1654, 1789
PkrI	GCNGC	6 cut(s)	346, 939, 1234, 1416, 1777, 1857
Ple19I	CGATCG	1 cut(s)	221
PleI	GAGTC	1 cut(s)	379
PpsI	GAGTC	1 cut(s)	379
PsiI	TTATAA	2 cut(s)	446, 540
PspFI	CCCAGC	1 cut(s)	1543
PspN4I	GGNNCC	1 cut(s)	1596
PstNI	CAGNNNCTG	1 cut(s)	1018
PsuI	RGATCY	1 cut(s)	928
PvuI	CGATCG	1 cut(s)	221
RsaI	GTAC	3 cut(s)	329, 1596, 1891
RsaNI	GTAC	3 cut(s)	328, 1595, 1890
RseI	CAYNNNNRTG	3 cut(s)	117, 1073, 1885
SatI	GCNGC	6 cut(s)	345, 938, 1233, 1415, 1776, 1856
Sau3AI	GATC	6 cut(s)	218, 549, 928, 1009, 1687, 1697
SchI	GAGTC	1 cut(s)	380
SduI	GDGCHC	2 cut(s)	1091, 1201
SfaNI	GCATC	5 cut(s)	727, 807, 949, 1489, 1530
Sfr274I	CTCGAG	1 cut(s)	599
SlaI	CTCGAG	1 cut(s)	599
SmiMI	CAYNNNNRTG	3 cut(s)	117, 1073, 1885
SmlI	CTYRAG	4 cut(s)	493, 599, 904, 1200
SmoI	CTYRAG	4 cut(s)	493, 599, 904, 1200
SphI	GCATGC	2 cut(s)	1166, 1322
SseBI	AGGCCT	2 cut(s)	313, 902
SspI	AATATT	1 cut(s)	429
SspMI	CTAG	5 cut(s)	228, 341, 656, 812, 1716
StuI	AGGCCT	2 cut(s)	313, 902
StyI	CCWWGG	3 cut(s)	483, 990, 1418
TaaI	ACNGT	3 cut(s)	1227, 1745, 1894
TaqI	TCGA	4 cut(s)	208, 221, 600, 1008
TatI	WGTACW	1 cut(s)	1889
TfiI	GAWTC	8 cut(s)	8, 415, 461, 584, 808, 1098, 1654, 1789
TscAI	CASTG	2 cut(s)	324, 972
TseI	GCWGC	6 cut(s)	344, 937, 1232, 1414, 1775, 1855
TspRI	CASTG	2 cut(s)	324, 972
Vha464I	CTTAAG	2 cut(s)	493, 904
XapI	RAATTY	4 cut(s)	1034, 1356, 1617, 1808
XbaI	TCTAGA	1 cut(s)	811
XceI	RCATGY	2 cut(s)	1166, 1322
XhoI	CTCGAG	1 cut(s)	599
XspI	CTAG	5 cut(s)	228, 341, 656, 812, 1716
Zsp2I	ATGCAT	2 cut(s)	1084, 1168

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.