MD08G1134900.v1.1

zinc-finger of the FCS-type, C2-C2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006492
Physical Location & Seq
Forward (+)
12828703 .. 12831419
2717 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1134900.v1.1.491

Sequence Viewer

Length: 1197 bp
ATGCTGAGGAAGAGAAGCAGGTCAGTTCAGAAGGATCAGCACCAAATGGGTCGTCTGCCAATTTCTGATGCTGCTGCTGGGTCTGATGTTTTAGGGCACAACCCTAAAAGCAACTCTTTTTTCAGTGTTCCTGGTCTGTTTGTGGGGTTGAGTACTAAGGGTTTATCAGATTATGATGCAGTTAGGAGCCCAACTTCTCCTCTAGATTTTAGGGTCTTTTCCAATCTAGGTTACCCCTTTAGGTCCCCAAGGTCCAACCAGGATGGGGGGCAGCAGAGGAGCTGGGGTAGCAGTAAAGTAGGATTAAGCATCATAGATTCTATTGATGATGTGAAATTTTTCGGGAAAGTTCCTCAGTCATCTGAGAGTAAGAACATTCTTTTTGGACCCGGAATGAGGATTAATAAAATCCCAGAGCCTCAGTCCAATGCCAAGTGTTTTGATTATTCACCGAAATCTCTGCCCAAGAACTATGCAATCTCCCCTCATTCCAAAATCAAATCTCCTTCACAGAAGGGTAGTTCTGATGTTCTTTTTGAAATCGGAGAAGCCCCATCAGAACCTGAGTCATTCGGGAAAATCCGGTCCTGTTCATTAGATTCTGGTAGAGCATTTTCGACCCTATCAGGATTGAGCAACCTTAACCCCAACTCCACTTCTGGAGGTTTTTGCCCGGAAAATTTAACCACCCATCAATTTATTGGAGGAAGCCCCAATTTAGCCACCCAAATGAAAACTGGATCCATCGAGTCTAGCAATGGGTTTGTCGGGTCTCTTTCAGCAAGTGAGATTGAGCTTTCTGAGGATTATACTTGTGTAATCTCTCATGGTGCCAATCCCAAAAAAACTCACATCTTTGGTGACTGCATTTTGGAATGTCACTCTGAGGATGTGAACAATTTTGGTAAGAATGTGGAAAAGGAAATTGGATTTGCCCACCCGGGTTCGAGCTTGGAGAATTTTGGTCACTACCCTTCAGACAATTTCCTGAGCTTCTGTTACTATTGTAACAAGAAATTGGAAGAGGGCAAAGATATATACATATACAGGGGTGAGAAAGCATTTTGCAGTTTGAGTTGCCGGTCGGAGGAAATTTTGATTGATGAGGAGTTGGAAAAATGCAATGACCAGTCTTCTGGGAAACCTCTCGAGTCGGATGAGGAACTTTTTGAAACTGGCTTGCTTGCCGATAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-FLZ

Protein Analysis

399

Amino Acids

43.42

Weight (kDa)

5.68

Isoelectric Point (pI)

61.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-FLZ PF04570 323 - 370 7.1e-22 zinc-finger of the FCS-type, C2-C2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 9
AccB1I GGYRCC 1 cut(s) 830
AclWI GGATC 3 cut(s) 42, 735, 748
AcsI RAATTY 4 cut(s) 335, 679, 958, 1092
AcuI CTGAAG 1 cut(s) 960
AfaI GTAC 1 cut(s) 154
AfiI CCNNNNNNNGG 3 cut(s) 265, 396, 1087
AgsI TTSAA 2 cut(s) 539, 1172
AjnI CCWGG 2 cut(s) 130, 258
AjuI GAANNNNNNNTTGG 2 cut(s) 909, 941
AluBI AGCT 4 cut(s) 282, 796, 951, 993
AluI AGCT 4 cut(s) 282, 796, 951, 993
Alw26I GTCTC 1 cut(s) 777
AlwI GGATC 3 cut(s) 42, 735, 748
AlwNI CAGNNNCTG 1 cut(s) 563
Ama87I CYCGRG 2 cut(s) 940, 1148
ApeKI GCWGC 3 cut(s) 71, 74, 271
ApoI RAATTY 4 cut(s) 335, 679, 958, 1092
ArsI GACNNNNNNTTYG 2 cut(s) 37, 69
AseI ATTAAT 1 cut(s) 402
Asp700I GAANNNNTTC 1 cut(s) 338
AspS9I GGNCC 4 cut(s) 243, 252, 386, 585
AsuC2I CCSGG 4 cut(s) 390, 674, 941, 942
AsuHPI GGTGA 3 cut(s) 441, 872, 1064
AvaI CYCGRG 2 cut(s) 940, 1148
AvaII GGWCC 4 cut(s) 243, 252, 386, 585
BaeGI GKGCMC 1 cut(s) 99
BamHI GGATCC 1 cut(s) 740
BanI GGYRCC 1 cut(s) 830
BanII GRGCYC 1 cut(s) 191
BbsI GAAGAC 1 cut(s) 1125
BbvCI CCTCAGC 1 cut(s) 5
BbvI GCAGC 3 cut(s) 58, 61, 283
BccI CCATC 4 cut(s) 257, 562, 699, 752
BcgI CGANNNNNNTGC 2 cut(s) 442, 476
BciT130I CCWGG 2 cut(s) 132, 260
BcnI CCSGG 4 cut(s) 390, 674, 941, 942
BcoDI GTCTC 1 cut(s) 777
BfaI CTAG 3 cut(s) 203, 227, 753
BfuAI ACCTGC 1 cut(s) 9
BisI GCNGC 3 cut(s) 72, 75, 272
BlsI GCNGC 3 cut(s) 73, 76, 273
BmcAI AGTACT 1 cut(s) 154
Bme1390I CCNGG 6 cut(s) 132, 260, 390, 674, 941, 942
Bme18I GGWCC 4 cut(s) 243, 252, 386, 585
BmeT110I CYCGRG 2 cut(s) 940, 1148
BmgT120I GGNCC 4 cut(s) 243, 252, 386, 585
BmiI GGNNCC 5 cut(s) 188, 245, 388, 742, 832
BmrFI CCNGG 6 cut(s) 132, 260, 390, 674, 941, 942
BmsI GCATC 3 cut(s) 58, 166, 318
BpiI GAAGAC 1 cut(s) 1125
BpmI CTGGAG 1 cut(s) 681
Bpu10I CCTNAGC 2 cut(s) 5, 989
BpuMI CCSGG 4 cut(s) 390, 674, 941, 942
BsaI GGTCTC 1 cut(s) 777
BsaJI CCNNGG 2 cut(s) 248, 940
BsaWI WCCGGW 1 cut(s) 582
BsaXI ACNNNNNCTCC 4 cut(s) 271, 301, 635, 665
Bsc4I CCNNNNNNNGG 3 cut(s) 265, 396, 1087
Bse118I RCCGGY 1 cut(s) 1080
Bse1I ACTGG 3 cut(s) 742, 1129, 1180
Bse3DI GCAATG 2 cut(s) 763, 1129
BseBI CCWGG 2 cut(s) 132, 260
BseDI CCNNGG 2 cut(s) 248, 940
BseGI GGATG 3 cut(s) 268, 895, 1162
BseLI CCNNNNNNNGG 3 cut(s) 265, 396, 1087
BseMI GCAATG 2 cut(s) 763, 1129
BseMII CTCAG 7 cut(s) 354, 368, 434, 555, 792, 876, 980
BseNI ACTGG 3 cut(s) 742, 1129, 1180
BseRI GAGGAG 3 cut(s) 189, 292, 1121
BseSI GKGCMC 1 cut(s) 99
BseXI GCAGC 3 cut(s) 58, 61, 283
BseYI CCCAGC 2 cut(s) 77, 282
Bsh1285I CGRYCG 1 cut(s) 1085
BshNI GGYRCC 1 cut(s) 830
BsiEI CGRYCG 1 cut(s) 1085
BsiHKCI CYCGRG 2 cut(s) 940, 1148
BsiSI CCGG 5 cut(s) 390, 583, 674, 941, 1081
BslFI GGGAC 1 cut(s) 229
BslI CCNNNNNNNGG 3 cut(s) 265, 396, 1087
BsmAI GTCTC 1 cut(s) 777
BsmFI GGGAC 1 cut(s) 229
Bso31I GGTCTC 1 cut(s) 777
BsoBI CYCGRG 2 cut(s) 940, 1148
Bsp1286I GDGCHC 2 cut(s) 99, 191
Bsp143I GATC 2 cut(s) 34, 740
BspCNI CTCAG 7 cut(s) 355, 367, 433, 556, 793, 877, 981
BspLI GGNNCC 5 cut(s) 188, 245, 388, 742, 832
BspMI ACCTGC 1 cut(s) 9
BspPI GGATC 3 cut(s) 42, 735, 748
BspT107I GGYRCC 1 cut(s) 830
BspTNI GGTCTC 1 cut(s) 777
BsrDI GCAATG 2 cut(s) 763, 1129
BsrFI RCCGGY 1 cut(s) 1080
BsrI ACTGG 3 cut(s) 742, 1129, 1180
BssAI RCCGGY 1 cut(s) 1080
BssECI CCNNGG 2 cut(s) 248, 940
BssMI GATC 2 cut(s) 34, 740
BssT1I CCWWGG 1 cut(s) 248
Bst2UI CCWGG 2 cut(s) 132, 260
Bst6I CTCTTC 2 cut(s) 5, 1017
BstC8I GCNNGC 2 cut(s) 1181, 1185
BstDEI CTNAG 9 cut(s) 5, 156, 354, 363, 420, 564, 801, 885, 989
BstEII GGTNACC 1 cut(s) 230
BstF5I GGATG 3 cut(s) 268, 895, 1162
BstKTI GATC 2 cut(s) 37, 743
BstMAI GTCTC 1 cut(s) 777
BstMBI GATC 2 cut(s) 34, 740
BstMCI CGRYCG 1 cut(s) 1085
BstMWI GCNNNNNNNGC 1 cut(s) 288
BstNI CCWGG 2 cut(s) 132, 260
BstPI GGTNACC 1 cut(s) 230
BstSCI CCNGG 6 cut(s) 130, 258, 388, 672, 939, 940
BstSLI GKGCMC 1 cut(s) 99
BstV1I GCAGC 3 cut(s) 58, 61, 283
BstV2I GAAGAC 1 cut(s) 1125
BstX2I RGATCY 1 cut(s) 740
BstXI CCANNNNNNTGG 1 cut(s) 1136
BstYI RGATCY 1 cut(s) 740
BtsCI GGATG 3 cut(s) 268, 895, 1162
BtsIMutI CAGTG 1 cut(s) 130
BveI ACCTGC 1 cut(s) 9
Cac8I GCNNGC 2 cut(s) 1181, 1185
CaiI CAGNNNCTG 1 cut(s) 563
Cfr10I RCCGGY 1 cut(s) 1080
Cfr13I GGNCC 4 cut(s) 243, 252, 386, 585
Cfr9I CCCGGG 1 cut(s) 940
Csp6I GTAC 1 cut(s) 153
CviAII CATG 1 cut(s) 827
CviQI GTAC 1 cut(s) 153
DdeI CTNAG 9 cut(s) 5, 156, 354, 363, 420, 564, 801, 885, 989
DpnI GATC 2 cut(s) 36, 742
DpnII GATC 2 cut(s) 34, 740
Eam1104I CTCTTC 2 cut(s) 5, 1017
EarI CTCTTC 2 cut(s) 5, 1017
Eco130I CCWWGG 1 cut(s) 248
Eco24I GRGCYC 1 cut(s) 191
Eco31I GGTCTC 1 cut(s) 777
Eco47I GGWCC 4 cut(s) 243, 252, 386, 585
Eco57I CTGAAG 1 cut(s) 960
Eco88I CYCGRG 2 cut(s) 940, 1148
Eco91I GGTNACC 1 cut(s) 230
EcoO109I RGGNCCY 1 cut(s) 243
EcoO65I GGTNACC 1 cut(s) 230
EcoRII CCWGG 2 cut(s) 130, 258
EcoT14I CCWWGG 1 cut(s) 248
EcoT38I GRGCYC 1 cut(s) 191
ErhI CCWWGG 1 cut(s) 248
FaeI CATG 1 cut(s) 830
FaiI YATR 9 cut(s) 174, 314, 474, 810, 828, 1037, 1039, 1043, 1045
FalI AAGNNNNNCTT 2 cut(s) 100, 132
FaqI GGGAC 1 cut(s) 229
FatI CATG 1 cut(s) 826
Fnu4HI GCNGC 3 cut(s) 72, 75, 272
FokI GGATG 3 cut(s) 275, 902, 1169
FriOI GRGCYC 1 cut(s) 191
Fsp4HI GCNGC 3 cut(s) 72, 75, 272
FspBI CTAG 3 cut(s) 203, 227, 753
GluI GCNGC 3 cut(s) 72, 75, 272
GsaI CCCAGC 2 cut(s) 81, 286
GsuI CTGGAG 1 cut(s) 681
HapII CCGG 5 cut(s) 390, 583, 674, 941, 1081
Hin1II CATG 1 cut(s) 830
HinfI GANTC 5 cut(s) 317, 566, 599, 749, 1151
HpaII CCGG 5 cut(s) 390, 583, 674, 941, 1081
HphI GGTGA 3 cut(s) 441, 872, 1064
Hpy166II GTNNAC 1 cut(s) 895
Hpy188III TCNNGA 7 cut(s) 203, 343, 574, 627, 660, 988, 1148
Hpy8I GTNNAC 1 cut(s) 895
HpyAV CCTTC 4 cut(s) 25, 508, 516, 984
HpyCH4V TGCA 5 cut(s) 179, 476, 867, 1068, 1122
HpyF10VI GCNNNNNNNGC 1 cut(s) 288
HpyF3I CTNAG 9 cut(s) 5, 156, 354, 363, 420, 564, 801, 885, 989
Hsp92II CATG 1 cut(s) 830
Kzo9I GATC 2 cut(s) 34, 740
LmnI GCTCC 2 cut(s) 186, 279
Lsp1109I GCAGC 3 cut(s) 58, 61, 283
LweI GCATC 3 cut(s) 58, 166, 318
MaeI CTAG 3 cut(s) 203, 227, 753
MaeIII GTNAC 6 cut(s) 230, 860, 878, 965, 998, 1007
MalI GATC 2 cut(s) 36, 742
MboI GATC 2 cut(s) 34, 740
MboII GAAGA 3 cut(s) 22, 1034, 1125
MflI RGATCY 1 cut(s) 740
MhlI GDGCHC 2 cut(s) 99, 191
MlyI GAGTC 3 cut(s) 575, 758, 1160
MmeI TCCRAC 4 cut(s) 279, 1065, 1092, 1134
MroXI GAANNNNTTC 1 cut(s) 338
MseI TTAA 4 cut(s) 305, 402, 642, 683
MslI CAYNNNNRTG 1 cut(s) 728
MspI CCGG 5 cut(s) 390, 583, 674, 941, 1081
MspR9I CCNGG 6 cut(s) 132, 260, 390, 674, 941, 942
MvaI CCWGG 2 cut(s) 132, 260
MwoI GCNNNNNNNGC 1 cut(s) 288
NciI CCSGG 4 cut(s) 390, 674, 941, 942
NdeII GATC 2 cut(s) 34, 740
NlaIII CATG 1 cut(s) 830
NlaIV GGNNCC 5 cut(s) 188, 245, 388, 742, 832
NmuCI GTSAC 3 cut(s) 860, 878, 965
PaeR7I CTCGAG 1 cut(s) 1148
PdmI GAANNNNTTC 1 cut(s) 338
PfeI GAWTC 2 cut(s) 317, 599
PkrI GCNGC 3 cut(s) 73, 76, 273
PleI GAGTC 3 cut(s) 574, 757, 1159
PpsI GAGTC 3 cut(s) 574, 757, 1159
PpuMI RGGWCCY 1 cut(s) 243
PshBI ATTAAT 1 cut(s) 402
Psp5II RGGWCCY 1 cut(s) 243
Psp6I CCWGG 2 cut(s) 130, 258
PspEI GGTNACC 1 cut(s) 230
PspFI CCCAGC 2 cut(s) 77, 282
PspGI CCWGG 2 cut(s) 130, 258
PspN4I GGNNCC 5 cut(s) 188, 245, 388, 742, 832
PspPI GGNCC 4 cut(s) 243, 252, 386, 585
PspPPI RGGWCCY 1 cut(s) 243
PstNI CAGNNNCTG 1 cut(s) 563
PsuI RGATCY 1 cut(s) 740
RsaI GTAC 1 cut(s) 154
RsaNI GTAC 1 cut(s) 153
RseI CAYNNNNRTG 1 cut(s) 728
SaqAI TTAA 4 cut(s) 305, 402, 642, 683
SatI GCNGC 3 cut(s) 72, 75, 272
Sau3AI GATC 2 cut(s) 34, 740
Sau96I GGNCC 4 cut(s) 243, 252, 386, 585
ScaI AGTACT 1 cut(s) 154
SchI GAGTC 3 cut(s) 575, 758, 1160
ScrFI CCNGG 6 cut(s) 132, 260, 390, 674, 941, 942
SduI GDGCHC 2 cut(s) 99, 191
SfaNI GCATC 3 cut(s) 58, 166, 318
Sfr274I CTCGAG 1 cut(s) 1148
SinI GGWCC 4 cut(s) 243, 252, 386, 585
SlaI CTCGAG 1 cut(s) 1148
SmaI CCCGGG 1 cut(s) 942
SmiMI CAYNNNNRTG 1 cut(s) 728
SmlI CTYRAG 1 cut(s) 1148
SmoI CTYRAG 1 cut(s) 1148
SspMI CTAG 3 cut(s) 203, 227, 753
StyD4I CCNGG 6 cut(s) 130, 258, 388, 672, 939, 940
StyI CCWWGG 1 cut(s) 248
TaqI TCGA 4 cut(s) 617, 747, 947, 1149
TatI WGTACW 1 cut(s) 152
TfiI GAWTC 2 cut(s) 317, 599
Tru1I TTAA 4 cut(s) 305, 402, 642, 683
Tru9I TTAA 4 cut(s) 305, 402, 642, 683
TscAI CASTG 1 cut(s) 130
TseFI GTSAC 3 cut(s) 860, 878, 965
TseI GCWGC 3 cut(s) 71, 74, 271
Tsp45I GTSAC 3 cut(s) 860, 878, 965
TspDTI ATGAA 2 cut(s) 582, 746
TspMI CCCGGG 1 cut(s) 940
TspRI CASTG 1 cut(s) 130
VpaK11BI GGWCC 4 cut(s) 243, 252, 386, 585
VspI ATTAAT 1 cut(s) 402
XapI RAATTY 4 cut(s) 335, 679, 958, 1092
XbaI TCTAGA 1 cut(s) 202
XcmI CCANNNNNNNNNTGG 2 cut(s) 698, 734
XhoI CTCGAG 1 cut(s) 1148
XmaI CCCGGG 1 cut(s) 940
XmnI GAANNNNTTC 1 cut(s) 338
XspI CTAG 3 cut(s) 203, 227, 753
ZrmI AGTACT 1 cut(s) 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.