MD15G1113700.v1.1

zinc-finger of the FCS-type, C2-C2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006492
Physical Location & Seq
Forward (+)
8025164 .. 8027840
2677 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1113700.v1.1.491

Sequence Viewer

Length: 1161 bp
ATGCTGAGGAAGAGAAGCAGGTCAGTTCAGAAGGATCAGCACCAAATGGGTCATCTGCCAATTTCTGATGCTGCCGCTGGGTCTGATGTTTTGGGGCACAACCCTAAAAGCAGCTCTTTTTTCAGTGTTCCTGGTCTGTTTGTAGGGTTGAGTCCTAAGGGTTTATCAGATTGTGATGCAGTTAGAAGCCCAACTTCTCCTCTAGATTTTAGGGTTTTTTCCAATCTGGGTAACCCCTATAGGTCACAAAGGTCCAACCATGATGGGGGACAGCAGAGGAGCTGGGGTAGCAGTAAAGTAGGATTAAGCATCATAGATTCTCTTGATGATGTGAAATCTTCCGGGAGAGTTCCTCGTTCGTCGGAGAGTAAGAACATTCTTTTTGGACCTGGAATGAGGATTAATAAAACCCCAGAGCCTCAGTCCAATGTCAAGCCTTTCGATTCTTCACCAAAATCTCTGCCCAAGAACTATGCAATTTTCCCTCATTCCAAAACCAAATCTCCTTTACAGAAGGGTAGTTCTGATGTTCTTTTTGAAATCGGAGAACCCCCATCAGAACCTGGGTCATTCGGGAAAATCCGGTCCTCTTCATTAGATTCCGGTAGAGCATTTTCGACTCTATTGGGATTGAGCAACCTTAACCCCAGCTCTACTTCAGGAAATTTTTGCCCGGAAAATTTAACCACCCAAATGAAGACTGGATCCATCGGGTCTAGCAATGGGTTTGTTGGGTCTCTCCCAGCAAGTGAGATTGAGCTTTCTGAGGATTATACTTGTGTAATCTCTCATGGTGCCAAACCCAAAAAAACTCACATCTTTGGTGACTGCATTTTGGAGTGTCATTCTGAGGATGTGAACAATTTTGGTAAGAATGAGGAAAAGGAAATTGGATTCACCCGCCCTGGTTCGAGCTTGGACAATTTTGGTCACTACCCTTTAGACAACTTCCTTAGCTTCTGCTACTATTGTAACAAGAAATTGGAAGAGGGCAAAGATATTTACATATACAGGGGTGAGCAAGCATTTTGCAGTTTGAGTTGCCGTTCGGAGGAGATTTTGATTGATGAGGCGTTGGAAAAATGCAATGACCAGTCTTCTGAGAAGCCTCTCGAGTCGTATGAGGAACTTTTCGAGACTGGCATGCTTGCAGATAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-FLZ

Protein Analysis

387

Amino Acids

42.16

Weight (kDa)

6.03

Isoelectric Point (pI)

63.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-FLZ PF04570 311 - 358 2.1e-21 zinc-finger of the FCS-type, C2-C2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 9
AccB1I GGYRCC 1 cut(s) 794
AciI CCGC 2 cut(s) 75, 901
AclWI GGATC 3 cut(s) 42, 699, 712
AcsI RAATTY 2 cut(s) 664, 679
AcuI CTGAAG 1 cut(s) 642
AfiI CCNNNNNNNGG 2 cut(s) 265, 1051
AgsI TTSAA 1 cut(s) 539
AjnI CCWGG 4 cut(s) 130, 388, 562, 904
AjuI GAANNNNNNNTTGG 2 cut(s) 873, 905
AluBI AGCT 6 cut(s) 114, 282, 651, 760, 915, 957
AluI AGCT 6 cut(s) 114, 282, 651, 760, 915, 957
Alw26I GTCTC 2 cut(s) 741, 1130
AlwI GGATC 3 cut(s) 42, 699, 712
AlwNI CAGNNNCTG 1 cut(s) 563
Ama87I CYCGRG 1 cut(s) 1112
ApeKI GCWGC 2 cut(s) 71, 111
ApoI RAATTY 2 cut(s) 664, 679
AseI ATTAAT 1 cut(s) 402
AspS9I GGNCC 3 cut(s) 252, 386, 585
AsuC2I CCSGG 2 cut(s) 343, 674
AsuHPI GGTGA 4 cut(s) 441, 836, 889, 1028
AvaI CYCGRG 1 cut(s) 1112
AvaII GGWCC 3 cut(s) 252, 386, 585
AxyI CCTNAGG 1 cut(s) 156
BaeGI GKGCMC 1 cut(s) 99
BamHI GGATCC 1 cut(s) 704
BanI GGYRCC 1 cut(s) 794
BbsI GAAGAC 2 cut(s) 704, 1089
BbvCI CCTCAGC 1 cut(s) 5
BbvI GCAGC 2 cut(s) 58, 123
BccI CCATC 3 cut(s) 257, 562, 716
BceAI ACGGC 1 cut(s) 1029
BciT130I CCWGG 4 cut(s) 132, 390, 564, 906
BcnI CCSGG 2 cut(s) 343, 674
BcoDI GTCTC 2 cut(s) 741, 1130
BfaI CTAG 2 cut(s) 203, 717
BfmI CTRYAG 1 cut(s) 238
BfuAI ACCTGC 1 cut(s) 9
BisI GCNGC 3 cut(s) 72, 75, 112
BlsI GCNGC 3 cut(s) 73, 76, 113
Bme1390I CCNGG 6 cut(s) 132, 343, 390, 564, 674, 906
Bme18I GGWCC 3 cut(s) 252, 386, 585
BmeT110I CYCGRG 1 cut(s) 1112
BmgT120I GGNCC 3 cut(s) 252, 386, 585
BmiI GGNNCC 2 cut(s) 706, 796
BmrFI CCNGG 6 cut(s) 132, 343, 390, 564, 674, 906
BmsI GCATC 3 cut(s) 58, 166, 318
BpiI GAAGAC 2 cut(s) 704, 1089
BplI GAGNNNNNCTC 2 cut(s) 337, 369
Bpu10I CCTNAGC 2 cut(s) 5, 953
BpuMI CCSGG 2 cut(s) 343, 674
BsaI GGTCTC 1 cut(s) 741
BsaJI CCNNGG 2 cut(s) 563, 904
BsaWI WCCGGW 2 cut(s) 582, 602
BsaXI ACNNNNNCTCC 4 cut(s) 271, 301, 487, 517
Bsc4I CCNNNNNNNGG 2 cut(s) 265, 1051
Bse1I ACTGG 3 cut(s) 706, 1093, 1144
Bse21I CCTNAGG 1 cut(s) 156
Bse3DI GCAATG 2 cut(s) 727, 1093
BseBI CCWGG 4 cut(s) 132, 390, 564, 906
BseDI CCNNGG 2 cut(s) 563, 904
BseGI GGATG 1 cut(s) 859
BseLI CCNNNNNNNGG 2 cut(s) 265, 1051
BseMI GCAATG 2 cut(s) 727, 1093
BseMII CTCAG 4 cut(s) 434, 756, 840, 1092
BseNI ACTGG 3 cut(s) 706, 1093, 1144
BseRI GAGGAG 3 cut(s) 189, 292, 1067
BseSI GKGCMC 1 cut(s) 99
BseXI GCAGC 2 cut(s) 58, 123
BseYI CCCAGC 4 cut(s) 77, 282, 647, 742
BshNI GGYRCC 1 cut(s) 794
BsiHKCI CYCGRG 1 cut(s) 1112
BsiSI CCGG 4 cut(s) 342, 583, 603, 674
BslFI GGGAC 1 cut(s) 282
BslI CCNNNNNNNGG 2 cut(s) 265, 1051
BsmAI GTCTC 2 cut(s) 741, 1130
BsmFI GGGAC 1 cut(s) 282
Bso31I GGTCTC 1 cut(s) 741
BsoBI CYCGRG 1 cut(s) 1112
Bsp1286I GDGCHC 1 cut(s) 99
Bsp143I GATC 2 cut(s) 34, 704
BspACI CCGC 2 cut(s) 75, 901
BspCNI CTCAG 4 cut(s) 433, 757, 841, 1093
BspLI GGNNCC 2 cut(s) 706, 796
BspMI ACCTGC 1 cut(s) 9
BspPI GGATC 3 cut(s) 42, 699, 712
BspT107I GGYRCC 1 cut(s) 794
BspTNI GGTCTC 1 cut(s) 741
BsrDI GCAATG 2 cut(s) 727, 1093
BsrI ACTGG 3 cut(s) 706, 1093, 1144
BssECI CCNNGG 2 cut(s) 563, 904
BssMI GATC 2 cut(s) 34, 704
Bst2UI CCWGG 4 cut(s) 132, 390, 564, 906
Bst6I CTCTTC 3 cut(s) 5, 595, 981
BstC8I GCNNGC 3 cut(s) 1023, 1145, 1149
BstDEI CTNAG 7 cut(s) 5, 156, 420, 765, 849, 953, 1101
BstEII GGTNACC 1 cut(s) 230
BstF5I GGATG 1 cut(s) 859
BstKTI GATC 2 cut(s) 37, 707
BstMAI GTCTC 2 cut(s) 741, 1130
BstMBI GATC 2 cut(s) 34, 704
BstMWI GCNNNNNNNGC 1 cut(s) 288
BstNI CCWGG 4 cut(s) 132, 390, 564, 906
BstNSI RCATGY 1 cut(s) 1147
BstPI GGTNACC 1 cut(s) 230
BstSCI CCNGG 6 cut(s) 130, 341, 388, 562, 672, 904
BstSFI CTRYAG 1 cut(s) 238
BstSLI GKGCMC 1 cut(s) 99
BstV1I GCAGC 2 cut(s) 58, 123
BstV2I GAAGAC 2 cut(s) 704, 1089
BstX2I RGATCY 1 cut(s) 704
BstYI RGATCY 1 cut(s) 704
Bsu36I CCTNAGG 1 cut(s) 156
BtsCI GGATG 1 cut(s) 859
BtsIMutI CAGTG 1 cut(s) 130
BveI ACCTGC 1 cut(s) 9
Cac8I GCNNGC 3 cut(s) 1023, 1145, 1149
CaiI CAGNNNCTG 1 cut(s) 563
Cfr13I GGNCC 3 cut(s) 252, 386, 585
CviAII CATG 3 cut(s) 260, 791, 1144
DdeI CTNAG 7 cut(s) 5, 156, 420, 765, 849, 953, 1101
DpnI GATC 2 cut(s) 36, 706
DpnII GATC 2 cut(s) 34, 704
Eam1104I CTCTTC 3 cut(s) 5, 595, 981
EarI CTCTTC 3 cut(s) 5, 595, 981
Eco31I GGTCTC 1 cut(s) 741
Eco47I GGWCC 3 cut(s) 252, 386, 585
Eco57I CTGAAG 1 cut(s) 642
Eco81I CCTNAGG 1 cut(s) 156
Eco88I CYCGRG 1 cut(s) 1112
Eco91I GGTNACC 1 cut(s) 230
EcoO65I GGTNACC 1 cut(s) 230
EcoRII CCWGG 4 cut(s) 130, 388, 562, 904
FaeI CATG 3 cut(s) 263, 794, 1147
FalI AAGNNNNNCTT 4 cut(s) 100, 132, 178, 210
FaqI GGGAC 1 cut(s) 282
FatI CATG 3 cut(s) 259, 790, 1143
FauI CCCGC 1 cut(s) 908
Fnu4HI GCNGC 3 cut(s) 72, 75, 112
FokI GGATG 1 cut(s) 866
Fsp4HI GCNGC 3 cut(s) 72, 75, 112
FspBI CTAG 2 cut(s) 203, 717
GluI GCNGC 3 cut(s) 72, 75, 112
GsaI CCCAGC 4 cut(s) 81, 286, 651, 746
HapII CCGG 4 cut(s) 342, 583, 603, 674
Hin1II CATG 3 cut(s) 263, 794, 1147
HinfI GANTC 7 cut(s) 151, 317, 443, 599, 619, 894, 1115
HpaII CCGG 4 cut(s) 342, 583, 603, 674
HphI GGTGA 4 cut(s) 441, 836, 889, 1028
Hpy166II GTNNAC 1 cut(s) 859
Hpy188III TCNNGA 6 cut(s) 203, 323, 574, 660, 1112, 1135
Hpy8I GTNNAC 1 cut(s) 859
Hpy99I CGWCG 1 cut(s) 364
HpyAV CCTTC 2 cut(s) 25, 508
HpyCH4V TGCA 6 cut(s) 179, 476, 831, 1032, 1086, 1151
HpyF10VI GCNNNNNNNGC 1 cut(s) 288
HpyF3I CTNAG 7 cut(s) 5, 156, 420, 765, 849, 953, 1101
Hsp92II CATG 3 cut(s) 263, 794, 1147
Kzo9I GATC 2 cut(s) 34, 704
LmnI GCTCC 1 cut(s) 279
Lsp1109I GCAGC 2 cut(s) 58, 123
LweI GCATC 3 cut(s) 58, 166, 318
MaeI CTAG 2 cut(s) 203, 717
MaeIII GTNAC 5 cut(s) 230, 243, 824, 929, 971
MalI GATC 2 cut(s) 36, 706
MboI GATC 2 cut(s) 34, 704
MboII GAAGA 7 cut(s) 22, 330, 438, 582, 709, 998, 1089
MflI RGATCY 1 cut(s) 704
MhlI GDGCHC 1 cut(s) 99
MluCI AATT 8 cut(s) 60, 477, 664, 679, 862, 888, 922, 980
MlyI GAGTC 3 cut(s) 160, 613, 1124
MmeI TCCRAC 3 cut(s) 279, 342, 1056
MseI TTAA 4 cut(s) 305, 402, 642, 683
MslI CAYNNNNRTG 1 cut(s) 692
MspA1I CMGCKG 1 cut(s) 77
MspI CCGG 4 cut(s) 342, 583, 603, 674
MspR9I CCNGG 6 cut(s) 132, 343, 390, 564, 674, 906
MvaI CCWGG 4 cut(s) 132, 390, 564, 906
MwoI GCNNNNNNNGC 1 cut(s) 288
NciI CCSGG 2 cut(s) 343, 674
NdeII GATC 2 cut(s) 34, 704
NlaIII CATG 3 cut(s) 263, 794, 1147
NlaIV GGNNCC 2 cut(s) 706, 796
NmuCI GTSAC 3 cut(s) 243, 824, 929
NspI RCATGY 1 cut(s) 1147
PaeI GCATGC 1 cut(s) 1147
PaeR7I CTCGAG 1 cut(s) 1112
PfeI GAWTC 4 cut(s) 317, 443, 599, 894
PfoI TCCNGGA 1 cut(s) 341
PkrI GCNGC 3 cut(s) 73, 76, 113
PleI GAGTC 3 cut(s) 159, 613, 1123
PpsI GAGTC 3 cut(s) 159, 613, 1123
PshBI ATTAAT 1 cut(s) 402
Psp6I CCWGG 4 cut(s) 130, 388, 562, 904
PspEI GGTNACC 1 cut(s) 230
PspFI CCCAGC 4 cut(s) 77, 282, 647, 742
PspGI CCWGG 4 cut(s) 130, 388, 562, 904
PspN4I GGNNCC 2 cut(s) 706, 796
PspPI GGNCC 3 cut(s) 252, 386, 585
PstNI CAGNNNCTG 1 cut(s) 563
PsuI RGATCY 1 cut(s) 704
RseI CAYNNNNRTG 1 cut(s) 692
SaqAI TTAA 4 cut(s) 305, 402, 642, 683
SatI GCNGC 3 cut(s) 72, 75, 112
Sau3AI GATC 2 cut(s) 34, 704
Sau96I GGNCC 3 cut(s) 252, 386, 585
SchI GAGTC 3 cut(s) 160, 613, 1124
ScrFI CCNGG 6 cut(s) 132, 343, 390, 564, 674, 906
SduI GDGCHC 1 cut(s) 99
SfaNI GCATC 3 cut(s) 58, 166, 318
SfcI CTRYAG 1 cut(s) 238
Sfr274I CTCGAG 1 cut(s) 1112
SinI GGWCC 3 cut(s) 252, 386, 585
SlaI CTCGAG 1 cut(s) 1112
SmiMI CAYNNNNRTG 1 cut(s) 692
SmlI CTYRAG 1 cut(s) 1112
SmoI CTYRAG 1 cut(s) 1112
SphI GCATGC 1 cut(s) 1147
Sse9I AATT 8 cut(s) 60, 477, 664, 679, 862, 888, 922, 980
SsiI CCGC 2 cut(s) 75, 901
SspMI CTAG 2 cut(s) 203, 717
StyD4I CCNGG 6 cut(s) 130, 341, 388, 562, 672, 904
TaqI TCGA 5 cut(s) 441, 617, 911, 1113, 1134
TasI AATT 8 cut(s) 60, 477, 664, 679, 862, 888, 922, 980
TauI GCSGC 1 cut(s) 77
TfiI GAWTC 4 cut(s) 317, 443, 599, 894
Tru1I TTAA 4 cut(s) 305, 402, 642, 683
Tru9I TTAA 4 cut(s) 305, 402, 642, 683
TscAI CASTG 1 cut(s) 130
TseFI GTSAC 3 cut(s) 243, 824, 929
TseI GCWGC 2 cut(s) 71, 111
Tsp45I GTSAC 3 cut(s) 243, 824, 929
TspDTI ATGAA 2 cut(s) 582, 710
TspRI CASTG 1 cut(s) 130
VpaK11BI GGWCC 3 cut(s) 252, 386, 585
VspI ATTAAT 1 cut(s) 402
XapI RAATTY 2 cut(s) 664, 679
XbaI TCTAGA 1 cut(s) 202
XceI RCATGY 1 cut(s) 1147
XcmI CCANNNNNNNNNTGG 1 cut(s) 698
XhoI CTCGAG 1 cut(s) 1112
XspI CTAG 2 cut(s) 203, 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.