MD08G1148200.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006671
Physical Location & Seq
Forward (+)
14905167 .. 14908456
3290 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1148200.v1.1.491

Sequence Viewer

Length: 1788 bp
ATGCAAAGGCTCGTCAAGACCGCCATTTTTACCGCCAACAGAAGAGAAGCAGAGCAGAGCTGCTTGGCGCTCCTCCAAGGGTGCGACGCCGTTTCAAAGCTCTCCCAGATCCACGCCCACGTTTTGAAATCGGGGCTCCAAAACAATCCGCTTGTACTCACCAAGTTCGCCGCCACGGCCTCCGACCTTAAAGCCATCGACTACGCCTCCTCCTTCCTCTTCTCCGGCGACGCCGATACGCATCTTTACGATTCGTTCCTCTTCAATACTGTAATTAGAGCTTACGCGCAGACCAGCCATGAAAAGCATAAAGCTTTGTGCTTTTACAATGTTATGGTTCAAGGTTCTGTTTTGCCTAATAAATTCACCTACCCTTTTGTCCTCAAGGCCTGTGCTGGAATTGGGGACTTGAATTTGGGGAAGTCGGTTCATGGGTCGGTGGTGAAATTCGGGTTCGATGCCGATGTTCATGTTTGTAACACTATGGTTCATATGTATAGTTGTTGTGGAGGAGGGATTGAGTTTGCCCGGAAGGTGTTTGATGAAATGCCCAAGTTGGACTCTGTTTCATGGAGTGCGATGATTGGCGGGTATGTGCGTGTGGGGTGGTCGACGGATTCAGTTGAGTTGTTTAGGGAAATGCAGATGGCGGGAGTTAAGCCGGATGAGATAACTATGGTCTCCGTTCTGTCTGCGTGTACGGATTTAGGTGCACTTGAGCTTGGGAAGTGGGTTGAGTCTTACATTGAGAAAGAAGGGATTCATAAATCTGTGGAGCTTTGTAATGCGCTCATCGACATGTTTGCAAAGTGCGGGAATGTGGATAAAGCTTTGAAATTGTTTAGAAACATGAGTGGGAGAACAATTGTTTCTTGGACTTCTGTGATTGATGGTCTGGCAATGCACGGCCGCGGAATGGAAGCCATTGCTTTGTTCGAGGAAATGATTGGGACTGGGGTAGCACCTGATGATGTTGCTTTCTTAGGGTTGCTTTCTGCGTGTAGTCATTCTGGGCTAGTTGAAAAGGGTAAGAGGTACTTCAGTTCAATGGTAGAAAAGTTTCAAATTGTACCTAAGATAGAACACTATGGATGCATGGTGGATATGTTTTGCAGGGCCGGACATGTCAAAGAAGCATCGGAGTTTATCCAAAAGATGCCGATTGAGCCAAACCCTATAGTCATGCGAACCCTGATCAGTGCTTGTCATGCACACGGTGAACTCAAGCTTGGAGAGAGTATCACCAAGCAGTTAATCAGGAGTGAACCTATGCAGGAGTCAAACTATGTCCTGCTTTCCAACATTTATGCAAAAATGAACCATTGGGACAAGAAAACCAAGATTAGAGAGGCGATGGACAAGAAGGGGATGAAAAAGATCCCTGGGAGCACTATGATCGAGCTAGACCACGAAATTTACGAATTTGTGGCTGGAGATAAATCGCATAGACAGTGCAAAGAGATTTACGAGATGGTAGAGGAGATGGGGAGGAAGATGAAGAAAGCTGGATATGTTCCCACTACATCAGAGGTCTTGCTAGATATCAATGAAGAAGATAAAGAGGATGCTTTGAATAGGCATAGTGAAAAGTTAGCCATTGCTTTTGCCCTCCTGAATACGCCTCCAGGAACTCCTATTCGAATAGTGAAGAACTTACGAGTTTGCGATGATTGCCACTCTGCTACTAAGTTCATCTCTAAAGTTTATAATCGAGAAATAGTGGTGAGGGATAGGAACAGGTTTCACCATTTCAAGGATGGTATGTGTTCTTGTAGAGATTTCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2 PPR_3

Protein Analysis

596

Amino Acids

66.6

Weight (kDa)

7.28

Isoelectric Point (pI)

38.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 84 - 132 3.8e-07 PPR repeat family
PPR_2 PF13041 187 - 233 3.7e-08 PPR repeat family
PPR PF01535 189 - 219 4.1e-06 PPR repeat
PPR_2 PF13041 288 - 335 8.9e-09 PPR repeat family
PPR PF01535 290 - 319 1.8e-06 PPR repeat
E_motif PF20431 405 - 467 5.1e-15 E motif
Eplus_motif PF20430 472 - 499 1.4e-06 E+ motif
DYW_deaminase PF14432 503 - 595 8e-43 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1707
AccI GTMKAC 1 cut(s) 611
AccII CGCG 2 cut(s) 287, 912
AciI CCGC 9 cut(s) 21, 33, 149, 171, 588, 650, 813, 910, 912
AclWI GGATC 2 cut(s) 103, 1372
AcoI YGGCCR 1 cut(s) 907
AcsI RAATTY 5 cut(s) 362, 412, 446, 1413, 1421
AcuI CTGAAG 1 cut(s) 1024
AcyI GRCGYC 2 cut(s) 87, 231
AdeI CACNNNGTG 1 cut(s) 1217
AfaI GTAC 4 cut(s) 156, 700, 1037, 1071
AfiI CCNNNNNNNGG 2 cut(s) 916, 1753
AflIII ACRYGT 2 cut(s) 798, 1123
AjnI CCWGG 2 cut(s) 1381, 1624
AjuI GAANNNNNNNTTGG 2 cut(s) 1212, 1244
Alw21I GWGCWC 2 cut(s) 715, 1391
Alw26I GTCTC 1 cut(s) 685
Alw44I GTGCAC 1 cut(s) 711
AlwI GGATC 2 cut(s) 103, 1372
AoxI GGCC 4 cut(s) 177, 387, 907, 1116
ApaLI GTGCAC 1 cut(s) 711
ApeKI GCWGC 1 cut(s) 60
ApoI RAATTY 5 cut(s) 362, 412, 446, 1413, 1421
Asp700I GAANNNNTTC 2 cut(s) 759, 1059
AspLEI GCGC 3 cut(s) 70, 289, 790
AspS9I GGNCC 1 cut(s) 1116
AsuC2I CCSGG 1 cut(s) 529
AsuHPI GGTGA 7 cut(s) 151, 358, 454, 1229, 1234, 1735, 1736
AsuII TTCGAA 1 cut(s) 1639
BaeGI GKGCMC 1 cut(s) 715
BaeI ACNNNNGTAYC 2 cut(s) 1027, 1060
BanII GRGCYC 1 cut(s) 138
Bbv12I GWGCWC 2 cut(s) 715, 1391
BbvI GCAGC 1 cut(s) 47
BccI CCATC 7 cut(s) 203, 640, 884, 1348, 1465, 1477, 1751
BceAI ACGGC 3 cut(s) 74, 192, 922
BcgI CGANNNNNNTGC 4 cut(s) 785, 819, 1378, 1412
BciT130I CCWGG 2 cut(s) 1383, 1626
BclI TGATCA 1 cut(s) 1194
BcnI CCSGG 1 cut(s) 529
BcoDI GTCTC 1 cut(s) 685
BfaI CTAG 3 cut(s) 1016, 1403, 1538
BfmI CTRYAG 1 cut(s) 1176
BfoI RGCGCY 1 cut(s) 71
BglI GCCNNNNNGGC 1 cut(s) 176
BisI GCNGC 3 cut(s) 61, 171, 910
BlsI GCNGC 3 cut(s) 62, 172, 911
Bme1390I CCNGG 3 cut(s) 529, 1383, 1626
BmgT120I GGNCC 1 cut(s) 1116
BmiI GGNNCC 1 cut(s) 137
BmrFI CCNGG 3 cut(s) 529, 1383, 1626
BmrI ACTGGG 1 cut(s) 963
BmsI GCATC 6 cut(s) 250, 448, 1082, 1145, 1146, 1555
BmuI ACTGGG 1 cut(s) 963
BpmI CTGGAG 2 cut(s) 1452, 1608
Bpu14I TTCGAA 1 cut(s) 1639
BpuEI CTTGAG 3 cut(s) 368, 737, 1208
BpuMI CCSGG 1 cut(s) 529
BsaBI GATNNNNATC 1 cut(s) 240
BsaHI GRCGYC 2 cut(s) 87, 231
BsaI GGTCTC 1 cut(s) 685
BsaJI CCNNGG 5 cut(s) 76, 174, 910, 1381, 1382
BsaXI ACNNNNNCTCC 4 cut(s) 191, 194, 221, 224
Bsc4I CCNNNNNNNGG 2 cut(s) 916, 1753
Bse1I ACTGG 1 cut(s) 958
Bse3DI GCAATG 3 cut(s) 906, 924, 1596
Bse8I GATNNNNATC 1 cut(s) 240
BseBI CCWGG 2 cut(s) 1383, 1626
BseDI CCNNGG 5 cut(s) 76, 174, 910, 1381, 1382
BseGI GGATG 5 cut(s) 670, 1097, 1374, 1570, 1762
BseJI GATNNNNATC 1 cut(s) 240
BseLI CCNNNNNNNGG 2 cut(s) 916, 1753
BseMI GCAATG 3 cut(s) 906, 924, 1596
BseNI ACTGG 1 cut(s) 958
BseRI GAGGAG 4 cut(s) 62, 199, 525, 1493
BseSI GKGCMC 1 cut(s) 715
BseX3I CGGCCG 1 cut(s) 907
BseXI GCAGC 1 cut(s) 47
Bsh1236I CGCG 2 cut(s) 287, 912
Bsh1285I CGRYCG 1 cut(s) 910
BshFI GGCC 4 cut(s) 179, 389, 909, 1118
BsiEI CGRYCG 1 cut(s) 910
BsiHKAI GWGCWC 2 cut(s) 715, 1391
BsiSI CCGG 4 cut(s) 225, 529, 662, 1119
BslFI GGGAC 3 cut(s) 419, 964, 1340
BslI CCNNNNNNNGG 2 cut(s) 916, 1753
BsmAI GTCTC 1 cut(s) 685
BsmFI GGGAC 3 cut(s) 419, 964, 1340
BsnI GGCC 4 cut(s) 179, 389, 909, 1118
Bso31I GGTCTC 1 cut(s) 685
Bsp119I TTCGAA 1 cut(s) 1639
Bsp1286I GDGCHC 3 cut(s) 138, 715, 1391
Bsp143I GATC 4 cut(s) 108, 1194, 1377, 1395
BspACI CCGC 9 cut(s) 21, 33, 149, 171, 588, 650, 813, 910, 912
BspANI GGCC 4 cut(s) 179, 389, 909, 1118
BspFNI CGCG 2 cut(s) 287, 912
BspLI GGNNCC 1 cut(s) 137
BspPI GGATC 2 cut(s) 103, 1372
BspT104I TTCGAA 1 cut(s) 1639
BspTNI GGTCTC 1 cut(s) 685
BsrDI GCAATG 3 cut(s) 906, 924, 1596
BsrI ACTGG 1 cut(s) 958
BssECI CCNNGG 5 cut(s) 76, 174, 910, 1381, 1382
BssMI GATC 4 cut(s) 108, 1194, 1377, 1395
BssNI GRCGYC 2 cut(s) 87, 231
BssT1I CCWWGG 1 cut(s) 76
Bst2UI CCWGG 2 cut(s) 1383, 1626
Bst4CI ACNGT 3 cut(s) 271, 1217, 1452
Bst6I CTCTTC 3 cut(s) 37, 224, 266
BstACI GRCGYC 2 cut(s) 87, 231
BstBI TTCGAA 1 cut(s) 1639
BstDEI CTNAG 3 cut(s) 982, 1074, 1686
BstDSI CCRYGG 2 cut(s) 174, 910
BstF5I GGATG 5 cut(s) 670, 1097, 1374, 1570, 1762
BstFNI CGCG 2 cut(s) 287, 912
BstH2I RGCGCY 1 cut(s) 71
BstHHI GCGC 3 cut(s) 70, 289, 790
BstKTI GATC 4 cut(s) 111, 1197, 1380, 1398
BstMAI GTCTC 1 cut(s) 685
BstMBI GATC 4 cut(s) 108, 1194, 1377, 1395
BstMCI CGRYCG 1 cut(s) 910
BstMWI GCNNNNNNNGC 4 cut(s) 176, 1165, 1208, 1671
BstNI CCWGG 2 cut(s) 1383, 1626
BstNSI RCATGY 2 cut(s) 802, 1127
BstSCI CCNGG 3 cut(s) 527, 1381, 1624
BstSFI CTRYAG 1 cut(s) 1176
BstSLI GKGCMC 1 cut(s) 715
BstUI CGCG 2 cut(s) 287, 912
BstV1I GCAGC 1 cut(s) 47
BstX2I RGATCY 2 cut(s) 108, 1377
BstYI RGATCY 2 cut(s) 108, 1377
BstZI CGGCCG 1 cut(s) 907
BsuRI GGCC 4 cut(s) 179, 389, 909, 1118
BtgI CCRYGG 2 cut(s) 174, 910
BtgZI GCGATG 3 cut(s) 593, 1367, 1680
BtsCI GGATG 5 cut(s) 670, 1097, 1374, 1570, 1762
BtsIMutI CAGTG 2 cut(s) 1204, 1457
CfoI GCGC 3 cut(s) 70, 289, 790
Cfr13I GGNCC 1 cut(s) 1116
Cfr42I CCGCGG 1 cut(s) 913
CseI GACGC 2 cut(s) 95, 239
Csp6I GTAC 4 cut(s) 155, 699, 1036, 1070
CviQI GTAC 4 cut(s) 155, 699, 1036, 1070
DdeI CTNAG 3 cut(s) 982, 1074, 1686
DpnI GATC 4 cut(s) 110, 1196, 1379, 1397
DpnII GATC 4 cut(s) 108, 1194, 1377, 1395
DraIII CACNNNGTG 1 cut(s) 1217
EaeI YGGCCR 1 cut(s) 907
EagI CGGCCG 1 cut(s) 907
Eam1104I CTCTTC 3 cut(s) 37, 224, 266
EarI CTCTTC 3 cut(s) 37, 224, 266
EclXI CGGCCG 1 cut(s) 907
Eco130I CCWWGG 1 cut(s) 76
Eco147I AGGCCT 1 cut(s) 389
Eco24I GRGCYC 1 cut(s) 138
Eco31I GGTCTC 1 cut(s) 685
Eco32I GATATC 1 cut(s) 1543
Eco52I CGGCCG 1 cut(s) 907
Eco57I CTGAAG 1 cut(s) 1024
EcoRII CCWGG 2 cut(s) 1381, 1624
EcoRV GATATC 1 cut(s) 1543
EcoT14I CCWWGG 1 cut(s) 76
EcoT22I ATGCAT 1 cut(s) 1097
EcoT38I GRGCYC 1 cut(s) 138
ErhI CCWWGG 1 cut(s) 76
FalI AAGNNNNNCTT 2 cut(s) 1022, 1054
FaqI GGGAC 3 cut(s) 419, 964, 1340
FauI CCCGC 3 cut(s) 581, 643, 806
FauNDI CATATG 1 cut(s) 492
FbaI TGATCA 1 cut(s) 1194
FblI GTMKAC 1 cut(s) 611
Fnu4HI GCNGC 3 cut(s) 61, 171, 910
FokI GGATG 5 cut(s) 677, 1104, 1381, 1577, 1769
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 3 cut(s) 61, 171, 910
FspBI CTAG 3 cut(s) 1016, 1403, 1538
GlaI GCGC 3 cut(s) 69, 288, 789
GluI GCNGC 3 cut(s) 61, 171, 910
GsuI CTGGAG 2 cut(s) 1452, 1608
HaeII RGCGCY 1 cut(s) 71
HaeIII GGCC 4 cut(s) 179, 389, 909, 1118
HapII CCGG 4 cut(s) 225, 529, 662, 1119
HgaI GACGC 2 cut(s) 95, 239
HhaI GCGC 3 cut(s) 70, 289, 790
Hin1I GRCGYC 2 cut(s) 87, 231
Hin6I GCGC 3 cut(s) 68, 287, 788
HinP1I GCGC 3 cut(s) 68, 287, 788
HincII GTYRAC 1 cut(s) 612
HindII GTYRAC 1 cut(s) 612
HindIII AAGCTT 3 cut(s) 312, 828, 1226
HinfI GANTC 6 cut(s) 251, 560, 617, 737, 760, 1277
HpaII CCGG 4 cut(s) 225, 529, 662, 1119
HphI GGTGA 7 cut(s) 151, 358, 454, 1229, 1234, 1735, 1736
Hpy166II GTNNAC 5 cut(s) 612, 699, 713, 1220, 1265
Hpy188I TCNGA 3 cut(s) 184, 1141, 1528
Hpy188III TCNNGA 4 cut(s) 16, 1258, 1612, 1712
Hpy8I GTNNAC 5 cut(s) 612, 699, 713, 1220, 1265
Hpy99I CGWCG 3 cut(s) 89, 233, 616
HpyAV CCTTC 4 cut(s) 223, 526, 749, 1357
HpyCH4III ACNGT 3 cut(s) 271, 1217, 1452
HpyCH4IV ACGT 1 cut(s) 120
HpyF10VI GCNNNNNNNGC 4 cut(s) 176, 1165, 1208, 1671
HpyF3I CTNAG 3 cut(s) 982, 1074, 1686
HpySE526I ACGT 1 cut(s) 120
Hsp92I GRCGYC 2 cut(s) 87, 231
HspAI GCGC 3 cut(s) 68, 287, 788
Ksp22I TGATCA 1 cut(s) 1194
KspI CCGCGG 1 cut(s) 913
Kzo9I GATC 4 cut(s) 108, 1194, 1377, 1395
LmnI GCTCC 4 cut(s) 75, 141, 775, 1386
Lsp1109I GCAGC 1 cut(s) 47
LweI GCATC 6 cut(s) 250, 448, 1082, 1145, 1146, 1555
MaeI CTAG 3 cut(s) 1016, 1403, 1538
MaeII ACGT 1 cut(s) 120
MaeIII GTNAC 1 cut(s) 476
MalI GATC 4 cut(s) 110, 1196, 1379, 1397
MboI GATC 4 cut(s) 108, 1194, 1377, 1395
MboII GAAGA 8 cut(s) 54, 211, 253, 1504, 1510, 1562, 1565, 1660
MfeI CAATTG 1 cut(s) 864
MflI RGATCY 2 cut(s) 108, 1377
MhlI GDGCHC 3 cut(s) 138, 715, 1391
MlyI GAGTC 3 cut(s) 554, 746, 1286
MmeI TCCRAC 3 cut(s) 207, 537, 1323
Mph1103I ATGCAT 1 cut(s) 1097
MroXI GAANNNNTTC 2 cut(s) 759, 1059
MseI TTAA 3 cut(s) 189, 657, 1253
MslI CAYNNNNRTG 1 cut(s) 797
MspA1I CMGCKG 1 cut(s) 912
MspI CCGG 4 cut(s) 225, 529, 662, 1119
MspR9I CCNGG 3 cut(s) 529, 1383, 1626
MunI CAATTG 1 cut(s) 864
MvaI CCWGG 2 cut(s) 1383, 1626
MvnI CGCG 2 cut(s) 287, 912
MwoI GCNNNNNNNGC 4 cut(s) 176, 1165, 1208, 1671
NciI CCSGG 1 cut(s) 529
NdeI CATATG 1 cut(s) 492
NdeII GATC 4 cut(s) 108, 1194, 1377, 1395
NlaIV GGNNCC 1 cut(s) 137
NsiI ATGCAT 1 cut(s) 1097
NspI RCATGY 2 cut(s) 802, 1127
NspV TTCGAA 1 cut(s) 1639
PasI CCCWGGG 1 cut(s) 1382
PceI AGGCCT 1 cut(s) 389
PciI ACATGT 2 cut(s) 798, 1123
PcsI WCGNNNNNNNCGW 1 cut(s) 1416
PdmI GAANNNNTTC 2 cut(s) 759, 1059
PfeI GAWTC 3 cut(s) 251, 617, 760
PfoI TCCNGGA 1 cut(s) 1624
PkrI GCNGC 3 cut(s) 62, 172, 911
PleI GAGTC 3 cut(s) 554, 745, 1285
PpsI GAGTC 3 cut(s) 554, 745, 1285
PscI ACATGT 2 cut(s) 798, 1123
PsiI TTATAA 1 cut(s) 1707
Psp6I CCWGG 2 cut(s) 1381, 1624
PspGI CCWGG 2 cut(s) 1381, 1624
PspN4I GGNNCC 1 cut(s) 137
PspPI GGNCC 1 cut(s) 1116
PsuI RGATCY 2 cut(s) 108, 1377
RsaI GTAC 4 cut(s) 156, 700, 1037, 1071
RsaNI GTAC 4 cut(s) 155, 699, 1036, 1070
RseI CAYNNNNRTG 1 cut(s) 797
SacII CCGCGG 1 cut(s) 913
SalI GTCGAC 1 cut(s) 610
SaqAI TTAA 3 cut(s) 189, 657, 1253
SatI GCNGC 3 cut(s) 61, 171, 910
Sau3AI GATC 4 cut(s) 108, 1194, 1377, 1395
Sau96I GGNCC 1 cut(s) 1116
SchI GAGTC 3 cut(s) 554, 746, 1286
ScrFI CCNGG 3 cut(s) 529, 1383, 1626
SduI GDGCHC 3 cut(s) 138, 715, 1391
SfaNI GCATC 6 cut(s) 250, 448, 1082, 1145, 1146, 1555
SfcI CTRYAG 1 cut(s) 1176
Sfr303I CCGCGG 1 cut(s) 913
SfuI TTCGAA 1 cut(s) 1639
SgrBI CCGCGG 1 cut(s) 913
SmiMI CAYNNNNRTG 1 cut(s) 797
SmlI CTYRAG 3 cut(s) 383, 716, 1223
SmoI CTYRAG 3 cut(s) 383, 716, 1223
SseBI AGGCCT 1 cut(s) 389
SsiI CCGC 9 cut(s) 21, 33, 149, 171, 588, 650, 813, 910, 912
SspMI CTAG 3 cut(s) 1016, 1403, 1538
StuI AGGCCT 1 cut(s) 389
StyD4I CCNGG 3 cut(s) 527, 1381, 1624
StyI CCWWGG 1 cut(s) 76
TaaI ACNGT 3 cut(s) 271, 1217, 1452
TaiI ACGT 1 cut(s) 123
TaqI TCGA 8 cut(s) 198, 456, 611, 795, 936, 1398, 1639, 1711
TatI WGTACW 1 cut(s) 154
TauI GCSGC 2 cut(s) 173, 912
TfiI GAWTC 3 cut(s) 251, 617, 760
Tru1I TTAA 3 cut(s) 189, 657, 1253
Tru9I TTAA 3 cut(s) 189, 657, 1253
TscAI CASTG 2 cut(s) 1204, 1457
TseI GCWGC 1 cut(s) 60
TspGWI ACGGA 3 cut(s) 629, 673, 716
TspRI CASTG 2 cut(s) 1204, 1457
VneI GTGCAC 1 cut(s) 711
XapI RAATTY 5 cut(s) 362, 412, 446, 1413, 1421
XceI RCATGY 2 cut(s) 802, 1127
XcmI CCANNNNNNNNNTGG 1 cut(s) 1754
XmiI GTMKAC 1 cut(s) 611
XmnI GAANNNNTTC 2 cut(s) 759, 1059
XspI CTAG 3 cut(s) 1016, 1403, 1538
Zsp2I ATGCAT 1 cut(s) 1097
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.