Rw0G010650

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00456
OG0006671
Physical Location & Seq
Reverse (-)
8492 .. 10291
1800 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G010650.1

Sequence Viewer

Length: 1794 bp
ATGCAATATTTCCAAACCCTGAAAAGAAGCAAGCTCATCATTAACACCAGAGAAGCAGAGCAGAGCTGCTTGGCTGTACTCCAACTGTGCGACGTCGTTCCAAAGCTGACGCAAATCCAAGCCCACATTGTCAAATTGGGCCTCCGAAACAACCCTTTAGTGCTGACCAAGTTGGCCTCCACCGCCTCCGACCTCAAAGCCATTGACCGCGCCTCTTCCTTCCTGTTCTCGCCGGATTCCGATACCCATTTATACGATGCGTTTCTCTTCAACACCATCATCAGGGCTTACGCTCAGTCCAGTCATGCAAAGCATAAAGCTTTGTGCTTTTACAGAGTTATGGTTAAGGGTGAAGTCTTGCCTAATAAATTCACCTACCCTTTTGTTCTCAAGTCCTGTGCCGACGTCGGGGAATTGAATTTGGGGAAGTCGGTTCACGGGTCGGTGGTGAAATTCGGGTTTGGTGATGATGTCCATGTTCGGAACACCATGGTTCATATGTATTGTTGCTGCGGTGGAGGGATTGAAAGTGCACGAAAGGTGTTTGATGAAATGCCTAACTTGGACTCTGTTTCGTGGAGCGCGATGATTGGAGGGTATGTGAGGGTGGGGTGGTCTAGTGATGCGGTGGAGTTGTTTAGGGAGATGCAGATGGCGGGAGTGAGGCCGGACGAGATTATTATGGTTTCGGTTTTGTCTGCGTGTACCGATTTGGGTGCGCTTGAGCTTGGCAAGTGGGTGGAGTCCTATATTGAGAAAGAGGGGATTCAGAAAAGTGTGGAGCTTTGTAATGCGCTCATTGACATGTTTGCGAAATGTGGCAATGTCGATAAGGCTTTGAAATTGTTTAGGAACATGAGTGGGAGAACAATTGTTTCTTGGACTTCTGTGATTGATGGTTTGGCAATGCATGGCCGCGGAGAAGAGGCTGTTTCTTTGTTTGAGGAAATGATTGGAGTCGGGGTGGAACCGGATGATGTTGCATTTATAGGACTGCTTTCGGCTTGCAGTCATTCCGGGTTGGTTGATAAGGGGAAGAGGTACTTCAGTATTATGGTGGAAAAATTTGAACGTGTACCTAAGATAGAACACTATGGATGCATGGTGGATACGTTATGCCGAGCTGGACATGTCAAGGAGGCATTGGAGTTTATTCAGAGAATGCCTATTGAGCCAAATCCAATAATTTTGCGAACCCTAATCAGTGCTTGTCGTGCACATGGTGAGCTCAAGCTTGGTGAGAGCATCACCAAGGAGTTAATCAGGGCTGAACCTATGCATGAGTCGAACTATGTCCTGCTTTCCAACATCTATGCAAAAATGAACCATTGGGAGAAGAAAACCAAGACTAGAGAGGCGATGGACAAGAAAGGGATGAAAAAGATCCCTGGGAGCACTATGATTGAGCTAGATAATGAAATTTACGAATTTGTGGCAGGGGATAAATCGCATAAACAGTCCAAAGAGATTTATGAAATGGTTGATGAAATGGGGAGGAAGATGAAGAGGGCTGGATATGTTCCCACTACATCAGAGGTTTTGCTAGACATTGATGAAGAAGATAAAGATAATGCTTTAAATAGGCATAGTGAAAAGTTGGCCATTGCTTTCGCCCTCCTGAACACTCCACCGGGAACTCCTATTCGCATAGTAAAGAATCTGCGGATTTGTGATGATTGCCACTCTGCTACTAAGTTCATCTCTAAGATTTATAATCGAGAAATAGTGGTGAGGGACCGGAACAGGTTTCACCATTTCAAGGGTGGTCTGTGTTCTTGTAGAGATTTCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2 PPR_3

Protein Analysis

597

Amino Acids

67.17

Weight (kDa)

7.8

Isoelectric Point (pI)

38.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 86 - 134 1.8e-06 PPR repeat family
PPR_2 PF13041 189 - 226 6.2e-07 PPR repeat family
PPR PF01535 191 - 221 1.5e-07 PPR repeat
PPR_2 PF13041 290 - 337 5.3e-10 PPR repeat family
PPR PF01535 292 - 322 3.7e-08 PPR repeat
E_motif PF20431 407 - 469 9e-14 E motif
Eplus_motif PF20430 474 - 501 1.1e-07 E+ motif
DYW_deaminase PF14432 505 - 597 1.7e-42 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1713
AatII GACGTC 2 cut(s) 96, 408
AccII CGCG 3 cut(s) 210, 584, 918
AciI CCGC 8 cut(s) 183, 208, 513, 626, 656, 916, 918, 1663
AclWI GGATC 1 cut(s) 1378
AcoI YGGCCR 2 cut(s) 913, 1599
AcsI RAATTY 6 cut(s) 368, 418, 452, 1064, 1419, 1427
AcuI CTGAAG 1 cut(s) 1030
AcyI GRCGYC 2 cut(s) 93, 405
AdeI CACNNNGTG 1 cut(s) 1223
AfaI GTAC 4 cut(s) 78, 706, 1043, 1077
AfiI CCNNNNNNNGG 3 cut(s) 282, 408, 1759
AflIII ACRYGT 3 cut(s) 804, 1072, 1129
AgsI TTSAA 6 cut(s) 271, 418, 527, 841, 1070, 1759
AjnI CCWGG 1 cut(s) 1387
Alw21I GWGCWC 4 cut(s) 535, 1219, 1230, 1397
Alw44I GTGCAC 2 cut(s) 531, 1215
AlwI GGATC 1 cut(s) 1378
AoxI GGCC 5 cut(s) 139, 174, 665, 913, 1599
ApaLI GTGCAC 2 cut(s) 531, 1215
ApeKI GCWGC 2 cut(s) 66, 510
ApoI RAATTY 6 cut(s) 368, 418, 452, 1064, 1419, 1427
AspLEI GCGC 4 cut(s) 212, 584, 721, 796
AspS9I GGNCC 2 cut(s) 139, 1735
AsuC2I CCSGG 2 cut(s) 1018, 1632
AsuHPI GGTGA 9 cut(s) 362, 364, 460, 476, 1235, 1240, 1250, 1741, 1742
AvaII GGWCC 1 cut(s) 1735
BaeGI GKGCMC 2 cut(s) 535, 1219
BaeI ACNNNNGTAYC 2 cut(s) 1033, 1066
BalI TGGCCA 1 cut(s) 1601
BanII GRGCYC 1 cut(s) 1230
Bbv12I GWGCWC 4 cut(s) 535, 1219, 1230, 1397
BbvI GCAGC 2 cut(s) 53, 497
BccI CCATC 4 cut(s) 284, 646, 890, 1354
BcgI CGANNNNNNTGC 2 cut(s) 698, 732
BciT130I CCWGG 1 cut(s) 1389
BciVI GTATCC 1 cut(s) 1102
BcnI CCSGG 2 cut(s) 1018, 1632
BfaI CTAG 4 cut(s) 618, 1350, 1409, 1544
BfuI GTATCC 1 cut(s) 1102
BisI GCNGC 3 cut(s) 67, 511, 916
BlsI GCNGC 3 cut(s) 68, 512, 917
Bme1390I CCNGG 3 cut(s) 1018, 1389, 1632
Bme18I GGWCC 1 cut(s) 1735
BmgT120I GGNCC 2 cut(s) 139, 1735
BmiI GGNNCC 2 cut(s) 969, 1736
BmrFI CCNGG 3 cut(s) 1018, 1389, 1632
BmsI GCATC 5 cut(s) 247, 613, 636, 1088, 1254
BpuEI CTTGAG 3 cut(s) 374, 743, 1214
BpuMI CCSGG 2 cut(s) 1018, 1632
BsaHI GRCGYC 2 cut(s) 93, 405
BsaJI CCNNGG 5 cut(s) 489, 916, 1251, 1387, 1388
BsaWI WCCGGW 2 cut(s) 970, 1737
BsaXI ACNNNNNCTCC 4 cut(s) 585, 615, 948, 978
Bsc4I CCNNNNNNNGG 3 cut(s) 282, 408, 1759
Bse1I ACTGG 1 cut(s) 300
Bse3DI GCAATG 3 cut(s) 829, 912, 1602
BseBI CCWGG 1 cut(s) 1389
BseDI CCNNGG 5 cut(s) 489, 916, 1251, 1387, 1388
BseGI GGATG 3 cut(s) 979, 1103, 1380
BseLI CCNNNNNNNGG 3 cut(s) 282, 408, 1759
BseMI GCAATG 3 cut(s) 829, 912, 1602
BseMII CTCAG 1 cut(s) 308
BseNI ACTGG 1 cut(s) 300
BseSI GKGCMC 2 cut(s) 535, 1219
BseXI GCAGC 2 cut(s) 53, 497
Bsh1236I CGCG 3 cut(s) 210, 584, 918
BshFI GGCC 5 cut(s) 141, 176, 667, 915, 1601
BsiHKAI GWGCWC 4 cut(s) 535, 1219, 1230, 1397
BsiSI CCGG 6 cut(s) 233, 668, 971, 1017, 1631, 1738
BslFI GGGAC 1 cut(s) 1748
BslI CCNNNNNNNGG 3 cut(s) 282, 408, 1759
BsmFI GGGAC 1 cut(s) 1748
BsmI GAATGC 1 cut(s) 1167
BsnI GGCC 5 cut(s) 141, 176, 667, 915, 1601
Bsp1286I GDGCHC 4 cut(s) 535, 1219, 1230, 1397
Bsp143I GATC 1 cut(s) 1383
Bsp19I CCATGG 1 cut(s) 489
BspACI CCGC 8 cut(s) 183, 208, 513, 626, 656, 916, 918, 1663
BspANI GGCC 5 cut(s) 141, 176, 667, 915, 1601
BspCNI CTCAG 1 cut(s) 307
BspFNI CGCG 3 cut(s) 210, 584, 918
BspLI GGNNCC 2 cut(s) 969, 1736
BspPI GGATC 1 cut(s) 1378
BsrDI GCAATG 3 cut(s) 829, 912, 1602
BsrI ACTGG 1 cut(s) 300
BssECI CCNNGG 5 cut(s) 489, 916, 1251, 1387, 1388
BssMI GATC 1 cut(s) 1383
BssNI GRCGYC 2 cut(s) 93, 405
BssT1I CCWWGG 2 cut(s) 489, 1251
Bst2UI CCWGG 1 cut(s) 1389
Bst4CI ACNGT 2 cut(s) 87, 1458
Bst6I CTCTTC 5 cut(s) 220, 272, 918, 1031, 1499
BstACI GRCGYC 2 cut(s) 93, 405
BstC8I GCNNGC 2 cut(s) 32, 1006
BstDEI CTNAG 4 cut(s) 294, 1080, 1692, 1704
BstDSI CCRYGG 2 cut(s) 489, 916
BstF5I GGATG 3 cut(s) 979, 1103, 1380
BstFNI CGCG 3 cut(s) 210, 584, 918
BstHHI GCGC 4 cut(s) 212, 584, 721, 796
BstKTI GATC 1 cut(s) 1386
BstMBI GATC 1 cut(s) 1383
BstMWI GCNNNNNNNGC 3 cut(s) 182, 1171, 1214
BstNI CCWGG 1 cut(s) 1389
BstNSI RCATGY 2 cut(s) 808, 1133
BstSCI CCNGG 3 cut(s) 1016, 1387, 1630
BstSLI GKGCMC 2 cut(s) 535, 1219
BstUI CGCG 3 cut(s) 210, 584, 918
BstV1I GCAGC 2 cut(s) 53, 497
BstX2I RGATCY 1 cut(s) 1383
BstYI RGATCY 1 cut(s) 1383
BsuI GTATCC 1 cut(s) 1102
BsuRI GGCC 5 cut(s) 141, 176, 667, 915, 1601
BtgI CCRYGG 2 cut(s) 489, 916
BtgZI GCGATG 2 cut(s) 599, 1373
BtsCI GGATG 3 cut(s) 979, 1103, 1380
BtsIMutI CAGTG 1 cut(s) 1210
Cac8I GCNNGC 2 cut(s) 32, 1006
CfoI GCGC 4 cut(s) 212, 584, 721, 796
Cfr13I GGNCC 2 cut(s) 139, 1735
Cfr42I CCGCGG 1 cut(s) 919
CseI GACGC 1 cut(s) 118
Csp6I GTAC 4 cut(s) 77, 705, 1042, 1076
CviQI GTAC 4 cut(s) 77, 705, 1042, 1076
DdeI CTNAG 4 cut(s) 294, 1080, 1692, 1704
DpnI GATC 1 cut(s) 1385
DpnII GATC 1 cut(s) 1383
DraI TTTAAA 1 cut(s) 1578
DraIII CACNNNGTG 1 cut(s) 1223
EaeI YGGCCR 2 cut(s) 913, 1599
Eam1104I CTCTTC 5 cut(s) 220, 272, 918, 1031, 1499
EarI CTCTTC 5 cut(s) 220, 272, 918, 1031, 1499
Ecl136II GAGCTC 1 cut(s) 1228
Eco130I CCWWGG 2 cut(s) 489, 1251
Eco24I GRGCYC 1 cut(s) 1230
Eco47I GGWCC 1 cut(s) 1735
Eco53kI GAGCTC 1 cut(s) 1228
Eco57I CTGAAG 1 cut(s) 1030
EcoICRI GAGCTC 1 cut(s) 1228
EcoRII CCWGG 1 cut(s) 1387
EcoT14I CCWWGG 2 cut(s) 489, 1251
EcoT22I ATGCAT 3 cut(s) 912, 1103, 1281
EcoT38I GRGCYC 1 cut(s) 1230
ErhI CCWWGG 2 cut(s) 489, 1251
FalI AAGNNNNNCTT 2 cut(s) 1028, 1060
FaqI GGGAC 1 cut(s) 1748
FauI CCCGC 1 cut(s) 649
FauNDI CATATG 1 cut(s) 498
Fnu4HI GCNGC 3 cut(s) 67, 511, 916
FokI GGATG 3 cut(s) 986, 1110, 1387
FriOI GRGCYC 1 cut(s) 1230
Fsp4HI GCNGC 3 cut(s) 67, 511, 916
FspBI CTAG 4 cut(s) 618, 1350, 1409, 1544
GlaI GCGC 4 cut(s) 211, 583, 720, 795
GluI GCNGC 3 cut(s) 67, 511, 916
HaeIII GGCC 5 cut(s) 141, 176, 667, 915, 1601
HapII CCGG 6 cut(s) 233, 668, 971, 1017, 1631, 1738
HgaI GACGC 1 cut(s) 118
HhaI GCGC 4 cut(s) 212, 584, 721, 796
Hin1I GRCGYC 2 cut(s) 93, 405
Hin6I GCGC 4 cut(s) 210, 582, 719, 794
HinP1I GCGC 4 cut(s) 210, 582, 719, 794
HindIII AAGCTT 2 cut(s) 318, 1232
HinfI GANTC 7 cut(s) 236, 566, 743, 766, 957, 1283, 1657
HpaII CCGG 6 cut(s) 233, 668, 971, 1017, 1631, 1738
HphI GGTGA 9 cut(s) 362, 364, 460, 476, 1235, 1240, 1250, 1741, 1742
Hpy166II GTNNAC 5 cut(s) 436, 533, 705, 1076, 1217
Hpy188I TCNGA 7 cut(s) 146, 190, 241, 483, 771, 1158, 1534
Hpy188III TCNNGA 2 cut(s) 1618, 1718
Hpy8I GTNNAC 5 cut(s) 436, 533, 705, 1076, 1217
Hpy99I CGWCG 4 cut(s) 95, 98, 407, 410
HpyAV CCTTC 1 cut(s) 229
HpyCH4III ACNGT 2 cut(s) 87, 1458
HpyCH4IV ACGT 4 cut(s) 93, 405, 1072, 1112
HpyF10VI GCNNNNNNNGC 3 cut(s) 182, 1171, 1214
HpyF3I CTNAG 4 cut(s) 294, 1080, 1692, 1704
HpySE526I ACGT 4 cut(s) 93, 405, 1072, 1112
Hsp92I GRCGYC 2 cut(s) 93, 405
HspAI GCGC 4 cut(s) 210, 582, 719, 794
KspI CCGCGG 1 cut(s) 919
Kzo9I GATC 1 cut(s) 1383
LmnI GCTCC 3 cut(s) 579, 781, 1392
Lsp1109I GCAGC 2 cut(s) 53, 497
LweI GCATC 5 cut(s) 247, 613, 636, 1088, 1254
MaeI CTAG 4 cut(s) 618, 1350, 1409, 1544
MaeII ACGT 4 cut(s) 93, 405, 1072, 1112
MalI GATC 1 cut(s) 1385
MboI GATC 1 cut(s) 1383
MboII GAAGA 9 cut(s) 207, 259, 935, 1048, 1348, 1510, 1516, 1568, 1571
MfeI CAATTG 1 cut(s) 870
MflI RGATCY 1 cut(s) 1383
MhlI GDGCHC 4 cut(s) 535, 1219, 1230, 1397
MlsI TGGCCA 1 cut(s) 1601
MluNI TGGCCA 1 cut(s) 1601
MlyI GAGTC 4 cut(s) 560, 752, 966, 1292
MmeI TCCRAC 3 cut(s) 106, 213, 1329
Mox20I TGGCCA 1 cut(s) 1601
Mph1103I ATGCAT 3 cut(s) 912, 1103, 1281
MscI TGGCCA 1 cut(s) 1601
MseI TTAA 4 cut(s) 42, 345, 1259, 1577
MslI CAYNNNNRTG 1 cut(s) 803
Msp20I TGGCCA 1 cut(s) 1601
MspA1I CMGCKG 1 cut(s) 918
MspI CCGG 6 cut(s) 233, 668, 971, 1017, 1631, 1738
MspR9I CCNGG 3 cut(s) 1018, 1389, 1632
MunI CAATTG 1 cut(s) 870
Mva1269I GAATGC 1 cut(s) 1167
MvaI CCWGG 1 cut(s) 1389
MvnI CGCG 3 cut(s) 210, 584, 918
MwoI GCNNNNNNNGC 3 cut(s) 182, 1171, 1214
NciI CCSGG 2 cut(s) 1018, 1632
NcoI CCATGG 1 cut(s) 489
NdeI CATATG 1 cut(s) 498
NdeII GATC 1 cut(s) 1383
NlaIV GGNNCC 2 cut(s) 969, 1736
NmeAIII GCCGAG 1 cut(s) 1145
NsiI ATGCAT 3 cut(s) 912, 1103, 1281
NspI RCATGY 2 cut(s) 808, 1133
PasI CCCWGGG 1 cut(s) 1388
PciI ACATGT 2 cut(s) 804, 1129
PcsI WCGNNNNNNNCGW 1 cut(s) 581
PctI GAATGC 1 cut(s) 1167
PfeI GAWTC 3 cut(s) 236, 766, 1657
PkrI GCNGC 3 cut(s) 68, 512, 917
PleI GAGTC 4 cut(s) 560, 751, 965, 1291
PpsI GAGTC 4 cut(s) 560, 751, 965, 1291
PscI ACATGT 2 cut(s) 804, 1129
PsiI TTATAA 1 cut(s) 1713
Psp124BI GAGCTC 1 cut(s) 1230
Psp6I CCWGG 1 cut(s) 1387
PspGI CCWGG 1 cut(s) 1387
PspN4I GGNNCC 2 cut(s) 969, 1736
PspPI GGNCC 2 cut(s) 139, 1735
PsuI RGATCY 1 cut(s) 1383
RsaI GTAC 4 cut(s) 78, 706, 1043, 1077
RsaNI GTAC 4 cut(s) 77, 705, 1042, 1076
RseI CAYNNNNRTG 1 cut(s) 803
SacI GAGCTC 1 cut(s) 1230
SacII CCGCGG 1 cut(s) 919
SaqAI TTAA 4 cut(s) 42, 345, 1259, 1577
SatI GCNGC 3 cut(s) 67, 511, 916
Sau3AI GATC 1 cut(s) 1383
Sau96I GGNCC 2 cut(s) 139, 1735
SchI GAGTC 4 cut(s) 560, 752, 966, 1292
ScrFI CCNGG 3 cut(s) 1018, 1389, 1632
SduI GDGCHC 4 cut(s) 535, 1219, 1230, 1397
SfaNI GCATC 5 cut(s) 247, 613, 636, 1088, 1254
Sfr303I CCGCGG 1 cut(s) 919
SgrBI CCGCGG 1 cut(s) 919
SinI GGWCC 1 cut(s) 1735
SmiMI CAYNNNNRTG 1 cut(s) 803
SmlI CTYRAG 3 cut(s) 389, 722, 1229
SmoI CTYRAG 3 cut(s) 389, 722, 1229
SsiI CCGC 8 cut(s) 183, 208, 513, 626, 656, 916, 918, 1663
SspI AATATT 1 cut(s) 8
SspMI CTAG 4 cut(s) 618, 1350, 1409, 1544
SstI GAGCTC 1 cut(s) 1230
StyD4I CCNGG 3 cut(s) 1016, 1387, 1630
StyI CCWWGG 2 cut(s) 489, 1251
TaaI ACNGT 2 cut(s) 87, 1458
TaiI ACGT 4 cut(s) 96, 408, 1075, 1115
TaqI TCGA 3 cut(s) 828, 1286, 1717
TatI WGTACW 1 cut(s) 76
TauI GCSGC 1 cut(s) 918
TfiI GAWTC 3 cut(s) 236, 766, 1657
Tru1I TTAA 4 cut(s) 42, 345, 1259, 1577
Tru9I TTAA 4 cut(s) 42, 345, 1259, 1577
TscAI CASTG 1 cut(s) 1210
TseI GCWGC 2 cut(s) 66, 510
TspRI CASTG 1 cut(s) 1210
VneI GTGCAC 2 cut(s) 531, 1215
VpaK11BI GGWCC 1 cut(s) 1735
XapI RAATTY 6 cut(s) 368, 418, 452, 1064, 1419, 1427
XceI RCATGY 2 cut(s) 808, 1133
XcmI CCANNNNNNNNNTGG 1 cut(s) 1760
XspI CTAG 4 cut(s) 618, 1350, 1409, 1544
ZraI GACGTC 2 cut(s) 94, 406
Zsp2I ATGCAT 3 cut(s) 912, 1103, 1281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.