Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0010866

Physical Location & Seq

Genomic Coordinates Reverse (-)

20750350 .. 20755752

Gene Structure 5403 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1172400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2709 bp

ATGACGGCCTCCGTTGAGAACCGCCAGTTCCCCCAGCTCAACCCGCCGATAAACAGAGTCGTCCGCTCCTTCAACTTCTCTGATCCCACACCCCCGAAAAACACTCCCTTCGTCGACAGCAACGACTCGTCGTCCGACGACGAAAATGACGATAGCGTTGTCCAGGAATACACGGATTTAATCCGGAGAGGCAACAACGAATTAGAGCCGTCGATCTTGGACCCCAGAGATGAAGCCACGTCAGACAGCTGGGTGCGTCGTAACTCTTCAATGGTCCGCCTCACTGGAAAACATCCCTTCAACTGCGAACCGCCACTCACCCGCCTCATGCACCACGGATTCATCACCCCGGTCCCACTCCACTACGTCCGCAACCACGGCGTCGTCCCCAAGGCTACTTGGCAGGACTGGACGGTGGAGATCACCGGCCTTGTCCGCCGACCGACCCGGTTCACCATGGACCAGCTCGTACGTGAATTCAAGCCACGAGAGCTCCCAGTTACCCTTGTCTGCGCGGGAAACCGGAGGAAGGAACAGAATCTCGTCAAGCAGACAATCGGCTTCAACTGGGGTGCCGCTGGCATCTCCAACTCCGTGTGGCGCGGCGTCCCACTCTGCGACGTGCTCAAGCGGTGCGGGATCCGCAGCCGCAAGAACGAAGCCCTCAACGTGTGCTTTGAGGGCGCCGAGGACCTGCCCGGTGGCGGTGGAACCAAGTACGGCACGAGCTTGAGAAAGGAGGTGGTGATGGACCCCTCGAGGGATATTATCCTAGCATACATGCAAAACGGGGAGCTTTTGGAGCCTGATCACGGGTTTCCGGTACGGATCATAATACCCGGGTTTATCGGGGGTCGGATGGTGAAGTGGTTGAAGCGCATTGTCGTAACGACGTCGGAATCCGACAACTACTACCACTTCAAGGATAATCGAGTGCTCCCGTCGCACGTCGATGCGGAGCTAGCTAACGCTGAAGCGTGGTGGTACAAGCCGGAGTATATCATTAACGAGCTGAATATAAACTCCGTTATAACGACGCCGTGTCACGAGGAGATATTGCCAATCAACTCGTATACAACTCAGAGGCCGTATATGATGAAAGGCTACGCATACTCAGGCGGCGGGAAGAAAGTCACACGAGTGGAGATAACAATGGATGGCGGTGAAACGTGGCATGTGTGCACGTTAGACCACCAGGAGAAGCCAAACAAGTACGGGAAATACTGGTGCTGGTGTTTCTGGTCACTAGAAGTGGAGGTGCTCGACCTACTCGCTGCCAAAGAGATTGCAGTGCGAGCTTGGGACGAGACACTCAATACTCAACCCGAGAAACTCATTTGGAACGTCATGGGAATGATGAACAACTGCTGGTTTCGGGTGAAAACCAATGTGTGCAAACCACAAAGGGGAGAGATAGGACTAGTGTTTGAGCATCCAACTCAACCTGGAAACCAATCAGGCGGATGGATGGCCAAAGAAAAACGCTTGGACTCTGAATCCAACACAACCTTAAAGAAGAGTGTTTCAACACCCTTCATGAACACTAGTGCATCCAAGATGTACTCAATGTCAGATGTTAAGAAGCACAACTCTTCTCAATCTCCCTGGATCGTTGTCCATGGCCATGTCTACGACTGCACTAGCTTCCTCAACGACCACCCTGGTGGCGTTGATAGCATTCTCATCAACGCTGGCACTGACTGCACTGAAGAGTTTGACGCCATCCACTCGGAAAAGGCCAAGAAAATGATTGAGGATTATAGAATTGGAGAGTTGGTGTCCACGAATTATGCTTCGGATTCCTCATCTAGTTCTCCTAATAACTCCGTGCACGGACCACCAAGAACAACATCAGTGTTGGACCTTATTTCCTCTAACATGGCACAGTTAGCCCCAATCAAAGAAGTAACACATTCATTAAGTGTCGCGCTTATTCCGCGTGAGAAAATCTCATGTAAGCTCATAGCCAAGATCTCGATCTCTCACGACGTGAGGCTCTTCAGGTTCAGATTGCCTTCAGAAGACCAAATTTTGGGATTGCCAGTGGGAAAGCACATTTTCGTGTGTGCTACAATTGAGGACAAGCTTTGCATGAGGGCTTACACTCCAAGCAGCTCAGTTGATGAAGTTGGGTACTTTGATTTGGTGGTCAAGGTTTACTTCAAAAATGTGCACCCTAAGTTCCCAAATGGGGGAATCATGTCACAGTATTTGGATTCACTGCCAATAGGTGCCGCACTAGACATAAAGGGTCCCTTAGGGCACATAGAGTACACTGGTCGTGGTAATTTTTTGGTCAATGGCAAGCCAAAGTTTGCAAAGAAGCTTGCCATGCTGGCTGGTGGGACCGGGATCACCCCAATTTACCAAGTGGCTCAGGCCATTTTGAAGGACCCAGAAGATGAGACTGAAATGTATGTGGTGTATGCAAATCGGACGGAAGATGATATTTTGCTGAGGGAAGAGCTTGATGGTTGGGCTAAACAACATGAGAGGTTCAAGGTTTGGTATGTGGTGGAGAATGGCAAGGAAGGGTGGGAATACAGTGTGGGATTCATCACGGAGAGTGTGCTGAGAGAGCACATTCCCGATGGATCGGACGGTGCTCTTGCACTAGCATGCGGGCCCCCTCCGATGATTAAGTTTGCCGTGCAACCGAATTTGGAGAAGATGAACTATGATACCACAAATTCATTGCTGTTGTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001505 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0005886 GO:0006082 GO:0006807 GO:0006809 GO:0008150 GO:0008152 GO:0008940 GO:0009058 GO:0009314 GO:0009416 GO:0009605 GO:0009607 GO:0009608 GO:0009610 GO:0009620 GO:0009628 GO:0009703 GO:0009987 GO:0016020 GO:0016491 GO:0016661 GO:0017144 GO:0034641 GO:0042126 GO:0042128 GO:0042133 GO:0042136 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046209 GO:0046857 GO:0050896 GO:0051704 GO:0051707 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:0071941 GO:0071944 GO:0072593 GO:1903409 GO:2001057

KEGG Pathways

Metabolic & Signaling

ko00910 ko01120 map00910 map01120

Pfam Domains

Protein Families

Cyt-b5 FAD_binding_6 Mo-co_dimer NAD_binding_1 Oxidored_molyb

Protein Analysis

903

Amino Acids

101.61

Weight (kDa)

6.24

Isoelectric Point (pI)

43.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Oxidored_molyb	PF00174	126 - 305	3.5e-55	Oxidoreductase molybdopterin binding domain
Mo-co_dimer	PF03404	333 - 464	5.6e-56	Mo-co oxidoreductase dimerisation domain
Cyt-b5	PF00173	523 - 592	1.5e-26	Cytochrome b5-like Heme/Steroid binding domain
FAD_binding_6	PF00970	652 - 758	2.1e-36	Oxidoreductase FAD-binding domain
NAD_binding_1	PF00175	778 - 884	3e-35	Oxidoreductase NAD-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0010866)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G37130 AT1G77760
fragaria_vesca	FvH4_5g39010
malus_domestica	MD08G1172400.v1.1 MD15G1357900.v1.1
prunus_persica	Prupe.1G505400_v2.0.a1
pyrus_communis	pycom08g14700 pycom15g32000
rosa_chinensis	RchiOBHm_Chr7g0240971
rosa_laevigata	RLG00000000608
rosa_multiflora	Rmu_co8418311.1_g000001 Rmu_sc0041887.1_g000001 Rmu_ssc0000392.1_g000001
rosa_roxburghii	Rroxscaffold_3G00219350
rosa_rugosa	Rorug07G0341100
rosa_samantha	Rh7AG495900 Rh7BG467400 Rh7CG513400 Rh7DG480400
rosa_wichuraiana	Rw7G041900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1031
AatII	GACGTC	1 cut(s)	896
AbsI	CCTCGAGG	1 cut(s)	757
Acc36I	ACCTGC	1 cut(s)	702
AccB1I	GGYRCC	3 cut(s)	572, 683, 2231
AccB7I	CCANNNNNTGG	1 cut(s)	2032
AccBSI	CCGCTC	1 cut(s)	66
AccI	GTMKAC	3 cut(s)	114, 1073, 1629
AccII	CGCG	4 cut(s)	515, 603, 1928, 1939
AccIII	TCCGGA	1 cut(s)	183
AclWI	GGATC	7 cut(s)	77, 634, 647, 836, 1616, 2360, 2602
AcoI	YGGCCR	2 cut(s)	1470, 1621
AcsI	RAATTY	4 cut(s)	476, 2028, 2659, 2689
AcuI	CTGAAG	4 cut(s)	993, 1728, 1984, 2001
AcyI	GRCGYC	6 cut(s)	381, 606, 684, 893, 1037, 1719
AdeI	CACNNNGTG	1 cut(s)	1990
AfaI	GTAC	8 cut(s)	471, 719, 825, 986, 1214, 1562, 2135, 2273
AfiI	CCNNNNNNNGG	9 cut(s)	529, 704, 760, 812, 922, 1406, 2032, 2191, 2192
AflIII	ACRYGT	1 cut(s)	669
AhdI	GACNNNNNGTC	2 cut(s)	130, 1041
AhlI	ACTAGT	2 cut(s)	1420, 1544
AjiI	CACGTC	4 cut(s)	240, 622, 949, 1990
AjnI	CCWGG	5 cut(s)	162, 1194, 1444, 1604, 1660
AleI	CACNNNNGTG	2 cut(s)	1139, 1662
Alw21I	GWGCWC	9 cut(s)	495, 627, 939, 1184, 1263, 1833, 2175, 2583, 2608
Alw26I	GTCTC	2 cut(s)	1301, 2399
Alw44I	GTGCAC	3 cut(s)	1180, 1829, 2171
AlwI	GGATC	7 cut(s)	77, 634, 647, 836, 1616, 2360, 2602
Ama87I	CYCGRG	3 cut(s)	757, 839, 1325
Aor13HI	TCCGGA	1 cut(s)	183
AoxI	GGCC	8 cut(s)	6, 427, 1085, 1470, 1621, 1737, 2379, 2624
ApaI	GGGCCC	1 cut(s)	2628
ApaLI	GTGCAC	3 cut(s)	1180, 1829, 2171
ApeKI	GCWGC	3 cut(s)	645, 1274, 2112
ApoI	RAATTY	4 cut(s)	476, 2028, 2659, 2689
AspLEI	GCGC	5 cut(s)	515, 603, 686, 879, 1930
AsuC2I	CCSGG	6 cut(s)	350, 448, 699, 840, 841, 2350
AsuHPI	GGTGA	9 cut(s)	310, 337, 415, 445, 757, 874, 1175, 1390, 2347
AsuNHI	GCTAGC	1 cut(s)	961
AvaI	CYCGRG	3 cut(s)	757, 839, 1325
AxyI	CCTNAGG	1 cut(s)	2257
BaeGI	GKGCMC	5 cut(s)	1184, 1833, 2175, 2265, 2628
BalI	TGGCCA	2 cut(s)	1472, 1623
BamHI	GGATCC	1 cut(s)	639
BanI	GGYRCC	3 cut(s)	572, 683, 2231
BanII	GRGCYC	2 cut(s)	495, 2628
BauI	CACGAG	4 cut(s)	486, 724, 1046, 1137
BbsI	GAAGAC	1 cut(s)	2028
Bbv12I	GWGCWC	9 cut(s)	495, 627, 939, 1184, 1263, 1833, 2175, 2583, 2608
BbvCI	CCTCAGC	1 cut(s)	2456
BbvI	GCAGC	3 cut(s)	657, 1261, 2124
BccI	CCATC	8 cut(s)	742, 853, 1151, 1458, 1462, 1730, 2465, 2585
BceAI	ACGGC	7 cut(s)	21, 193, 394, 736, 1024, 1072, 2633
BciT130I	CCWGG	5 cut(s)	164, 1196, 1446, 1606, 1662
BclI	TGATCA	1 cut(s)	808
BcnI	CCSGG	6 cut(s)	350, 448, 699, 840, 841, 2350
BcoDI	GTCTC	2 cut(s)	1301, 2399
BcuI	ACTAGT	2 cut(s)	1420, 1544
BfaI	CTAG	9 cut(s)	773, 962, 1247, 1421, 1545, 1641, 1809, 2240, 2615
BfoI	RGCGCY	1 cut(s)	687
BfuAI	ACCTGC	1 cut(s)	702
BglI	GCCNNNNNGGC	1 cut(s)	2336
BglII	AGATCT	1 cut(s)	1971
BisI	GCNGC	8 cut(s)	576, 604, 646, 649, 1120, 1275, 2113, 2235
BlsI	GCNGC	8 cut(s)	577, 605, 647, 650, 1121, 1276, 2114, 2236
BmeRI	GACNNNNNGTC	2 cut(s)	130, 1041
BmeT110I	CYCGRG	3 cut(s)	757, 839, 1325
BmgBI	CACGTC	4 cut(s)	240, 622, 949, 1990
BmrI	ACTGGG	2 cut(s)	491, 577
BmsI	GCATC	4 cut(s)	591, 943, 1441, 1559
BmtI	GCTAGC	1 cut(s)	965
BmuI	ACTGGG	2 cut(s)	491, 577
BpiI	GAAGAC	1 cut(s)	2028
BplI	GAGNNNNNCTC	2 cut(s)	1934, 1966
Bpu10I	CCTNAGC	2 cut(s)	2376, 2456
BpuEI	CTTGAG	2 cut(s)	611, 751
BpuMI	CCSGG	6 cut(s)	350, 448, 699, 840, 841, 2350
BsaAI	YACGTR	1 cut(s)	473
BsaBI	GATNNNNATC	1 cut(s)	1976
BsaHI	GRCGYC	6 cut(s)	381, 606, 684, 893, 1037, 1719
BsaWI	WCCGGW	3 cut(s)	183, 522, 820
BsaXI	ACNNNNNCTCC	4 cut(s)	477, 507, 1761, 1791
Bsc4I	CCNNNNNNNGG	9 cut(s)	529, 704, 760, 812, 922, 1406, 2032, 2191, 2192
Bse118I	RCCGGY	1 cut(s)	425
Bse1I	ACTGG	8 cut(s)	25, 289, 413, 497, 572, 1229, 2042, 2281
Bse21I	CCTNAGG	1 cut(s)	2257
Bse3DI	GCAATG	1 cut(s)	2693
Bse8I	GATNNNNATC	1 cut(s)	1976
BseAI	TCCGGA	1 cut(s)	183
BseBI	CCWGG	5 cut(s)	164, 1196, 1446, 1606, 1662
BseGI	GGATG	8 cut(s)	292, 864, 1162, 1432, 1469, 1473, 1550, 1722
BseJI	GATNNNNATC	1 cut(s)	1976
BseLI	CCNNNNNNNGG	9 cut(s)	529, 704, 760, 812, 922, 1406, 2032, 2191, 2192
BseMI	GCAATG	1 cut(s)	2693
BseMII	CTCAG	6 cut(s)	1094, 1128, 2130, 2390, 2447, 2564
BseNI	ACTGG	8 cut(s)	25, 289, 413, 497, 572, 1229, 2042, 2281
BseRI	GAGGAG	1 cut(s)	1064
BseSI	GKGCMC	5 cut(s)	1184, 1833, 2175, 2265, 2628
BseXI	GCAGC	3 cut(s)	657, 1261, 2124
BseYI	CCCAGC	2 cut(s)	33, 249
BsgI	GTGCAG	2 cut(s)	1621, 1687
Bsh1236I	CGCG	4 cut(s)	515, 603, 1928, 1939
Bsh1285I	CGRYCG	1 cut(s)	443
BshFI	GGCC	8 cut(s)	8, 429, 1087, 1472, 1623, 1739, 2381, 2626
BshNI	GGYRCC	3 cut(s)	572, 683, 2231
BsiEI	CGRYCG	1 cut(s)	443
BsiHKAI	GWGCWC	9 cut(s)	495, 627, 939, 1184, 1263, 1833, 2175, 2583, 2608
BsiHKCI	CYCGRG	3 cut(s)	757, 839, 1325
BsiWI	CGTACG	1 cut(s)	469
BslFI	GGGAC	6 cut(s)	338, 371, 593, 1316, 2238, 2359
BslI	CCNNNNNNNGG	9 cut(s)	529, 704, 760, 812, 922, 1406, 2032, 2191, 2192
BsmAI	GTCTC	2 cut(s)	1301, 2399
BsmFI	GGGAC	6 cut(s)	338, 371, 593, 1316, 2238, 2359
BsmI	GAATGC	1 cut(s)	1677
BsnI	GGCC	8 cut(s)	8, 429, 1087, 1472, 1623, 1739, 2381, 2626
BsoBI	CYCGRG	3 cut(s)	757, 839, 1325
Bsp120I	GGGCCC	1 cut(s)	2624
Bsp13I	TCCGGA	1 cut(s)	183
Bsp19I	CCATGG	2 cut(s)	456, 1618
BspANI	GGCC	8 cut(s)	8, 429, 1087, 1472, 1623, 1739, 2381, 2626
BspCNI	CTCAG	6 cut(s)	1093, 1127, 2129, 2389, 2448, 2565
BspEI	TCCGGA	1 cut(s)	183
BspFNI	CGCG	4 cut(s)	515, 603, 1928, 1939
BspHI	TCATGA	1 cut(s)	1536
BspMI	ACCTGC	1 cut(s)	702
BspOI	GCTAGC	1 cut(s)	965
BspPI	GGATC	7 cut(s)	77, 634, 647, 836, 1616, 2360, 2602
BspQI	GCTCTTC	2 cut(s)	2003, 2457
BspT107I	GGYRCC	3 cut(s)	572, 683, 2231
BsrBI	CCGCTC	1 cut(s)	66
BsrDI	GCAATG	1 cut(s)	2693
BsrFI	RCCGGY	1 cut(s)	425
BsrI	ACTGG	8 cut(s)	25, 289, 413, 497, 572, 1229, 2042, 2281
BssAI	RCCGGY	1 cut(s)	425
BssNAI	GTATAC	1 cut(s)	1074
BssNI	GRCGYC	6 cut(s)	381, 606, 684, 893, 1037, 1719
BssSI	CACGAG	4 cut(s)	486, 724, 1046, 1137
BssT1I	CCWWGG	3 cut(s)	390, 456, 1618
Bst1107I	GTATAC	1 cut(s)	1074
Bst2BI	CACGAG	4 cut(s)	486, 724, 1046, 1137
Bst2UI	CCWGG	5 cut(s)	164, 1196, 1446, 1606, 1662
Bst4CI	ACNGT	5 cut(s)	415, 1887, 2208, 2546, 2603
Bst6I	CTCTTC	6 cut(s)	271, 1511, 1597, 1704, 2003, 2457
BstACI	GRCGYC	6 cut(s)	381, 606, 684, 893, 1037, 1719
BstAPI	GCANNNNNTGC	1 cut(s)	1701
BstBAI	YACGTR	1 cut(s)	473
BstC8I	GCNNGC	9 cut(s)	580, 963, 1296, 1693, 2306, 2328, 2337, 2620, 2624
BstDEI	CTNAG	8 cut(s)	1080, 1114, 2116, 2178, 2257, 2376, 2456, 2573
BstDSI	CCRYGG	4 cut(s)	334, 376, 456, 1618
BstF5I	GGATG	8 cut(s)	292, 864, 1162, 1432, 1469, 1473, 1550, 1722
BstFNI	CGCG	4 cut(s)	515, 603, 1928, 1939
BstH2I	RGCGCY	1 cut(s)	687
BstHHI	GCGC	5 cut(s)	515, 603, 686, 879, 1930
BstMAI	GTCTC	2 cut(s)	1301, 2399
BstMCI	CGRYCG	1 cut(s)	443
BstNI	CCWGG	5 cut(s)	164, 1196, 1446, 1606, 1662
BstNSI	RCATGY	3 cut(s)	784, 1178, 2622
BstSLI	GKGCMC	5 cut(s)	1184, 1833, 2175, 2265, 2628
BstUI	CGCG	4 cut(s)	515, 603, 1928, 1939
BstV1I	GCAGC	3 cut(s)	657, 1261, 2124
BstV2I	GAAGAC	1 cut(s)	2028
BstX2I	RGATCY	2 cut(s)	639, 1971
BstXI	CCANNNNNNTGG	1 cut(s)	1664
BstYI	RGATCY	2 cut(s)	639, 1971
BstZ17I	GTATAC	1 cut(s)	1074
Bsu36I	CCTNAGG	1 cut(s)	2257
BsuRI	GGCC	8 cut(s)	8, 429, 1087, 1472, 1623, 1739, 2381, 2626
BtgI	CCRYGG	4 cut(s)	334, 376, 456, 1618
BtrI	CACGTC	4 cut(s)	240, 622, 949, 1990
BtsCI	GGATG	8 cut(s)	292, 864, 1162, 1432, 1469, 1473, 1550, 1722
BtsI	GCAGTG	2 cut(s)	1296, 2219
BtsIMutI	CAGTG	9 cut(s)	282, 1296, 1695, 1704, 1860, 2049, 2219, 2274, 2551
BveI	ACCTGC	1 cut(s)	702
Cac8I	GCNNGC	9 cut(s)	580, 963, 1296, 1693, 2306, 2328, 2337, 2620, 2624
CciI	TCATGA	1 cut(s)	1536
CfoI	GCGC	5 cut(s)	515, 603, 686, 879, 1930
Cfr10I	RCCGGY	1 cut(s)	425
Cfr9I	CCCGGG	1 cut(s)	839
CseI	GACGC	5 cut(s)	245, 370, 595, 1045, 1727
Csp6I	GTAC	8 cut(s)	470, 718, 824, 985, 1213, 1561, 2134, 2272
CviQI	GTAC	8 cut(s)	470, 718, 824, 985, 1213, 1561, 2134, 2272
DdeI	CTNAG	8 cut(s)	1080, 1114, 2116, 2178, 2257, 2376, 2456, 2573
DinI	GGCGCC	1 cut(s)	685
DraIII	CACNNNGTG	1 cut(s)	1990
DriI	GACNNNNNGTC	2 cut(s)	130, 1041
EaeI	YGGCCR	2 cut(s)	1470, 1621
Eam1104I	CTCTTC	6 cut(s)	271, 1511, 1597, 1704, 2003, 2457
Eam1105I	GACNNNNNGTC	2 cut(s)	130, 1041
EarI	CTCTTC	6 cut(s)	271, 1511, 1597, 1704, 2003, 2457
EciI	GGCGGA	3 cut(s)	266, 425, 1476
Ecl136II	GAGCTC	1 cut(s)	493
Eco130I	CCWWGG	3 cut(s)	390, 456, 1618
Eco24I	GRGCYC	2 cut(s)	495, 2628
Eco53kI	GAGCTC	1 cut(s)	493
Eco57I	CTGAAG	4 cut(s)	993, 1728, 1984, 2001
Eco81I	CCTNAGG	1 cut(s)	2257
Eco88I	CYCGRG	3 cut(s)	757, 839, 1325
EcoICRI	GAGCTC	1 cut(s)	493
EcoO109I	RGGNCCY	4 cut(s)	691, 2252, 2392, 2625
EcoRI	GAATTC	1 cut(s)	476
EcoRII	CCWGG	5 cut(s)	162, 1194, 1444, 1604, 1660
EcoT14I	CCWWGG	3 cut(s)	390, 456, 1618
EcoT38I	GRGCYC	2 cut(s)	495, 2628
EgeI	GGCGCC	1 cut(s)	685
EheI	GGCGCC	1 cut(s)	685
ErhI	CCWWGG	3 cut(s)	390, 456, 1618
FalI	AAGNNNNNCTT	4 cut(s)	2144, 2176, 2240, 2272
FaqI	GGGAC	6 cut(s)	338, 371, 593, 1316, 2238, 2359
FauI	CCCGC	6 cut(s)	51, 329, 508, 629, 1115, 2615
FbaI	TGATCA	1 cut(s)	808
FblI	GTMKAC	3 cut(s)	114, 1073, 1629
Fnu4HI	GCNGC	8 cut(s)	576, 604, 646, 649, 1120, 1275, 2113, 2235
FokI	GGATG	8 cut(s)	279, 871, 1169, 1419, 1476, 1480, 1537, 1709
FriOI	GRGCYC	2 cut(s)	495, 2628
Fsp4HI	GCNGC	8 cut(s)	576, 604, 646, 649, 1120, 1275, 2113, 2235
FspBI	CTAG	9 cut(s)	773, 962, 1247, 1421, 1545, 1641, 1809, 2240, 2615
GlaI	GCGC	5 cut(s)	514, 602, 685, 878, 1929
GluI	GCNGC	8 cut(s)	576, 604, 646, 649, 1120, 1275, 2113, 2235
GsaI	CCCAGC	2 cut(s)	37, 253
HaeII	RGCGCY	1 cut(s)	687
HaeIII	GGCC	8 cut(s)	8, 429, 1087, 1472, 1623, 1739, 2381, 2626
HgaI	GACGC	5 cut(s)	245, 370, 595, 1045, 1727
HhaI	GCGC	5 cut(s)	515, 603, 686, 879, 1930
Hin1I	GRCGYC	6 cut(s)	381, 606, 684, 893, 1037, 1719
Hin6I	GCGC	5 cut(s)	513, 601, 684, 877, 1928
HinP1I	GCGC	5 cut(s)	513, 601, 684, 877, 1928
HincII	GTYRAC	1 cut(s)	115
HindII	GTYRAC	1 cut(s)	115
HindIII	AAGCTT	2 cut(s)	2084, 2324
HphI	GGTGA	9 cut(s)	310, 337, 415, 445, 757, 874, 1175, 1390, 2347
Hpy188III	TCNNGA	6 cut(s)	184, 1046, 1537, 1975, 1985, 2588
HpyAV	CCTTC	8 cut(s)	79, 118, 307, 523, 1543, 2025, 2383, 2525
HpyCH4III	ACNGT	5 cut(s)	415, 1887, 2208, 2546, 2603
HpyF3I	CTNAG	8 cut(s)	1080, 1114, 2116, 2178, 2257, 2376, 2456, 2573
Hsp92I	GRCGYC	6 cut(s)	381, 606, 684, 893, 1037, 1719
HspAI	GCGC	5 cut(s)	513, 601, 684, 877, 1928
KasI	GGCGCC	1 cut(s)	683
KflI	GGGWCCC	1 cut(s)	2252
Kpn2I	TCCGGA	1 cut(s)	183
Ksp22I	TGATCA	1 cut(s)	808
LguI	GCTCTTC	2 cut(s)	2003, 2457
LmnI	GCTCC	6 cut(s)	71, 498, 793, 802, 942, 958
Lsp1109I	GCAGC	3 cut(s)	657, 1261, 2124
LweI	GCATC	4 cut(s)	591, 943, 1441, 1559
MaeI	CTAG	9 cut(s)	773, 962, 1247, 1421, 1545, 1641, 1809, 2240, 2615
MaeIII	GTNAC	8 cut(s)	260, 499, 886, 1043, 1132, 1242, 1906, 2202
MbiI	CCGCTC	1 cut(s)	66
MfeI	CAATTG	1 cut(s)	2073
MflI	RGATCY	2 cut(s)	639, 1971
MlsI	TGGCCA	2 cut(s)	1472, 1623
MluNI	TGGCCA	2 cut(s)	1472, 1623
Mly113I	GGCGCC	1 cut(s)	684
MlyI	GAGTC	3 cut(s)	66, 119, 1484
MmeI	TCCRAC	8 cut(s)	159, 612, 836, 876, 927, 1460, 1524, 1839
Mox20I	TGGCCA	2 cut(s)	1472, 1623
MroI	TCCGGA	1 cut(s)	183
MscI	TGGCCA	2 cut(s)	1472, 1623
MseI	TTAA	6 cut(s)	179, 1005, 1511, 1578, 1919, 2640
MslI	CAYNNNNRTG	7 cut(s)	951, 1139, 1352, 1623, 1662, 2060, 2617
Msp20I	TGGCCA	2 cut(s)	1472, 1623
MspA1I	CMGCKG	2 cut(s)	249, 578
MunI	CAATTG	1 cut(s)	2073
Mva1269I	GAATGC	1 cut(s)	1677
MvaI	CCWGG	5 cut(s)	164, 1196, 1446, 1606, 1662
MvnI	CGCG	4 cut(s)	515, 603, 1928, 1939
NarI	GGCGCC	1 cut(s)	684
NciI	CCSGG	6 cut(s)	350, 448, 699, 840, 841, 2350
NcoI	CCATGG	2 cut(s)	456, 1618
NheI	GCTAGC	1 cut(s)	961
NmeAIII	GCCGAG	1 cut(s)	712
NmuCI	GTSAC	4 cut(s)	1043, 1132, 1242, 2202
NspI	RCATGY	3 cut(s)	784, 1178, 2622
OliI	CACNNNNGTG	2 cut(s)	1139, 1662
PaeI	GCATGC	1 cut(s)	2622
PaeR7I	CTCGAG	1 cut(s)	757
PagI	TCATGA	1 cut(s)	1536
PciSI	GCTCTTC	2 cut(s)	2003, 2457
PcsI	WCGNNNNNNNCGW	2 cut(s)	120, 147
PctI	GAATGC	1 cut(s)	1677
PfeI	GAWTC	8 cut(s)	339, 538, 899, 1496, 1799, 2196, 2216, 2553
Pfl23II	CGTACG	1 cut(s)	469
PflMI	CCANNNNNTGG	1 cut(s)	2032
PfoI	TCCNGGA	1 cut(s)	162
PkrI	GCNGC	8 cut(s)	577, 605, 647, 650, 1121, 1276, 2114, 2236
PleI	GAGTC	3 cut(s)	65, 119, 1484
PluTI	GGCGCC	1 cut(s)	687
PpsI	GAGTC	3 cut(s)	65, 119, 1484
Ppu21I	YACGTR	1 cut(s)	473
PpuMI	RGGWCCY	3 cut(s)	691, 2252, 2392
PsiI	TTATAA	1 cut(s)	1031
Psp124BI	GAGCTC	1 cut(s)	495
Psp5II	RGGWCCY	3 cut(s)	691, 2252, 2392
Psp6I	CCWGG	5 cut(s)	162, 1194, 1444, 1604, 1660
PspFI	CCCAGC	2 cut(s)	33, 249
PspGI	CCWGG	5 cut(s)	162, 1194, 1444, 1604, 1660
PspLI	CGTACG	1 cut(s)	469
PspOMI	GGGCCC	1 cut(s)	2624
PspPPI	RGGWCCY	3 cut(s)	691, 2252, 2392
PspXI	VCTCGAGB	1 cut(s)	757
PsuI	RGATCY	2 cut(s)	639, 1971
PvuII	CAGCTG	1 cut(s)	249
RsaI	GTAC	8 cut(s)	471, 719, 825, 986, 1214, 1562, 2135, 2273
RsaNI	GTAC	8 cut(s)	470, 718, 824, 985, 1213, 1561, 2134, 2272
RseI	CAYNNNNRTG	7 cut(s)	951, 1139, 1352, 1623, 1662, 2060, 2617
SacI	GAGCTC	1 cut(s)	495
SalI	GTCGAC	1 cut(s)	113
SapI	GCTCTTC	2 cut(s)	2003, 2457
SaqAI	TTAA	6 cut(s)	179, 1005, 1511, 1578, 1919, 2640
SatI	GCNGC	8 cut(s)	576, 604, 646, 649, 1120, 1275, 2113, 2235
SchI	GAGTC	3 cut(s)	66, 119, 1484
SfaNI	GCATC	4 cut(s)	591, 943, 1441, 1559
SfoI	GGCGCC	1 cut(s)	685
Sfr274I	CTCGAG	1 cut(s)	757
SlaI	CTCGAG	1 cut(s)	757
SmaI	CCCGGG	1 cut(s)	841
SmiMI	CAYNNNNRTG	7 cut(s)	951, 1139, 1352, 1623, 1662, 2060, 2617
SmlI	CTYRAG	3 cut(s)	626, 730, 757
SmoI	CTYRAG	3 cut(s)	626, 730, 757
SpeI	ACTAGT	2 cut(s)	1420, 1544
SphI	GCATGC	1 cut(s)	2622
SspDI	GGCGCC	1 cut(s)	683
SspMI	CTAG	9 cut(s)	773, 962, 1247, 1421, 1545, 1641, 1809, 2240, 2615
SstI	GAGCTC	1 cut(s)	495
StyI	CCWWGG	3 cut(s)	390, 456, 1618
TaaI	ACNGT	5 cut(s)	415, 1887, 2208, 2546, 2603
TaqI	TCGA	7 cut(s)	114, 212, 758, 931, 951, 1263, 1976
TaqII	GACCGA	1 cut(s)	457
TatI	WGTACW	2 cut(s)	1560, 2271
TauI	GCSGC	5 cut(s)	578, 606, 651, 1122, 2237
TfiI	GAWTC	8 cut(s)	339, 538, 899, 1496, 1799, 2196, 2216, 2553
Tru1I	TTAA	6 cut(s)	179, 1005, 1511, 1578, 1919, 2640
Tru9I	TTAA	6 cut(s)	179, 1005, 1511, 1578, 1919, 2640
TscAI	CASTG	9 cut(s)	289, 1296, 1702, 1711, 1860, 2049, 2226, 2281, 2551
TseFI	GTSAC	4 cut(s)	1043, 1132, 1242, 2202
TseI	GCWGC	3 cut(s)	645, 1274, 2112
Tsp45I	GTSAC	4 cut(s)	1043, 1132, 1242, 2202
TspGWI	ACGGA	9 cut(s)	188, 351, 583, 841, 1015, 1816, 1848, 2453, 2576
TspMI	CCCGGG	1 cut(s)	839
TspRI	CASTG	9 cut(s)	289, 1296, 1702, 1711, 1860, 2049, 2226, 2281, 2551
Van91I	CCANNNNNTGG	1 cut(s)	2032
VneI	GTGCAC	3 cut(s)	1180, 1829, 2171
XapI	RAATTY	4 cut(s)	476, 2028, 2659, 2689
XceI	RCATGY	3 cut(s)	784, 1178, 2622
XhoI	CTCGAG	1 cut(s)	757
XmaI	CCCGGG	1 cut(s)	839
XmiI	GTMKAC	3 cut(s)	114, 1073, 1629
XspI	CTAG	9 cut(s)	773, 962, 1247, 1421, 1545, 1641, 1809, 2240, 2615
ZraI	GACGTC	1 cut(s)	894

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.