Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0010866

Physical Location & Seq

Genomic Coordinates Forward (+)

65539281 .. 65544166

Gene Structure 4886 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G041900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2721 bp

ATGGCGGCCTCAGTCCAGCAGAACCGCCAGTTCCCTCACATCGACCCCGCCCTAAACGGCGTCGTCCGCTCCTCTTTCAAATCAAGTACTCCCAACCGCCACTTCCAAGACATCACTACATCCCCGAAAAGCTCGGCCCCGATCGATCACTACGACGAATCGTCCTCCAGCGACGACGAAAACGACGAGAGCATCGTAGAGTACATGGATTTGGTCCGGAGAGGCAACAGAGAATTAGAGCCGTCGATCTTGGACCCCAGAGATGAGGCCACGTCGGACAGCTGGGTGGAGCGCAACGCCAGCATGGTCCGCCTCACAGGGAAACACCCCTTCAACTCGGAGCCGCCGCTCAGCCGGCTCATGCACCACGGCTTCATCACTCCGGTCCCACTCCACTACGTCCGCAACCACGGCGTCGTCCCCAAGGCCACGTGGCACGACTGGACCGTCGAGGTCACCGGCCTCGTCAAGCGGCCCGCCCGGTTCACCATGGACCAGCTCACCACCGAGTTTCGCGCCCGCGAGCTCCCCGTCACCCTCGTTTGCGCGGGAAACCGCCGCAAGGAGCAGAACATGGTGAAGCAGACGATCGGATTCAACTGGGGCGCCGCCGGCGTCTCCACCTCCGTCTGGCGCGGCGTGCGCCTCCGCGACGTCCTCAAGCGGTGCGGGATCTACAGCCGCAAAGGCGGCGCCCTCAACGTGTGCTTCGAAGGCGCCGAGGACCTGCCAGGTGGCGGTGGGTCCAAGTACGCCACCAGTCTGAGAAAAGAGGTGGCCATGGACGAGAGCAGGGATATCATACTTGCTTACATGCAAAACGGCGAGCGTTTGGCCCCCGATCACGGGTTTCCGGTTCGGGTGATCATACCTGGATTTATAGGGGGGCGGATGGTGAAATGGCTCAAACGCATCGTCGTGACGACGAAGGAGTCGGACTGTTATTATCATTACAAGGACAACAGGGTCCTTCCCTCGCATGTGGATGCCGAGCTGGCCAACGCTGAAGCGTGGTGGTACAAGCCGGAGTACATAATCAACGAGCTGAACATAAACTCGGTCATTACGACGCCGTCTCACGAGGAGATATTGTCGATAAATTCGTGGACGAGGCCGTACACCCTCAAAGGCTACGCATATTCCGGAGGGGGTAAGAAAGTGACACGAGTGGAGGTGACTCTAGACGGTGGGGAGACCTGGCACGTCTGCGATCTGGACCACCAAGAGAAGCCGAACAAATATGGGAAATACTGGTGCTGGTGCTTCTGGTCACTGGACGTGGAAGTGCTCAACTTACTGGTAGCCAAGGAGATTGCGGTGAGGGCTTGGGACGAAACCCACAACACTCAGCCCGAAAAGCTCATCTGGAATGTCATGGGAATGATGAACAACTGTTGGTTCCGAGTGAAAATCAATGTGTTGCCACACAGAGGAGAGATTGGAATAGTGTTTGAGCATCCAACCAAACCCGGAAACCAATCCGGCGGGTGGATGGCAAAAGACAGACGCTTGGAGACCGAATCCAACGCAACCCTCAAGAAGAGTGTCTCTTCACCCTTCATGAACACTTTATCCAAGGTCTACTCAATGTCCGAAGTCAAGAGGCATAACACCCCGCAGTCTACATGGATTGTTGTCCACGGTCATGTCTACGACTGCACTAGATTCCTCAATGACCACCCGGGCGGTGCTGATAGCATTTTAATCAATGCTGGAACAGATTGCACCGAAGAGTTTGAAGCAATCCATTCCGACAAGGCCAAGAAAATGCTTGAGGAATATAGAATAGGCGAGGTGGTCGGTACCACAGGTTACGCCTCGGACTCTAGCTCCCCTAATAATACCGTCCATGGGCCACCAAGAATATCTACTGTGGACTCTATGTCTTCTCTTACGAATTTTTTGGCTCCTATCAAAGAAATCACACTAAAAAGCGTCGCGCTCAATCCGCGCGAGAAAATTCCATGCAAGCTTGTGACCAAGACCTCGATTTCACATGACGTGAGGATATTCAAATTTGCATTGCCTTCAGAAGACCAAGGTCTAGGGTTGCCAGTGGGCAAGCACATATTCTTGTGTGCTACAATTGAGGGCAAGCTTTGCATGAGGGCTTACACACCAACCAGCTCGATTGATGAAGTTGGGTATTTTGATCTGGTGGTCAAGGTGTACTTTAAGAATGTGCACCCTCGGTTCCCTAATGGTGGGCTCATGTCACAGTACTTGGACTCTCTCCCAATTGGGTCGTCTCTAGACGTGAAGGGTCCATTGGGTCACATAGAGTACAACGGTAAAGGTAACTTTTTGGTCCACGGTAAACCCAAGTTTGCAAAGAAACTCGCCATGATTGCCGGAGGGACGGGGATCACACCCATTTACCAAGTAGCACAAGCCATCCTGAAAGACCCGGAGGACCACACCGAAATGCATGTCGTATATGCAAACCGAACCGAAGAGGACATTTTGTTGAGAGAAGAGCTTGACGGTTGGGCTAAAGAGCATGAAAGGTTCAATGTGTGGTATGTGGTGGAGAATGGTAAGGAAGGCTGGGAATACAGTGTGGGATTCATCACGGAGAGCATACTGAGGGAGCATGTTCCCGATGGATCAGACGGTTCTCTTGCGTTGGCGTGCGGGCCCCCACCAATGATCCAGTTTGCCGTGCAGCCAAATCTGGAGAAGATGAACTATGATGTCAAGAATTCGTTGCTGATATTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001505 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0005886 GO:0006082 GO:0006807 GO:0006809 GO:0008150 GO:0008152 GO:0008940 GO:0009058 GO:0009314 GO:0009416 GO:0009605 GO:0009607 GO:0009608 GO:0009610 GO:0009620 GO:0009628 GO:0009703 GO:0009987 GO:0016020 GO:0016491 GO:0016661 GO:0017144 GO:0034641 GO:0042126 GO:0042128 GO:0042133 GO:0042136 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046209 GO:0046857 GO:0050896 GO:0051704 GO:0051707 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:0071941 GO:0071944 GO:0072593 GO:1903409 GO:2001057

KEGG Pathways

Metabolic & Signaling

ko00910 ko01120 map00910 map01120

Pfam Domains

Protein Families

Cyt-b5 FAD_binding_6 Mo-co_dimer NAD_binding_1 Oxidored_molyb

Protein Analysis

906

Amino Acids

101.88

Weight (kDa)

6.57

Isoelectric Point (pI)

43.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Oxidored_molyb	PF00174	137 - 316	1.2e-55	Oxidoreductase molybdopterin binding domain
Mo-co_dimer	PF03404	344 - 473	4.3e-58	Mo-co oxidoreductase dimerisation domain
Cyt-b5	PF00173	530 - 599	1.8e-27	Cytochrome b5-like Heme/Steroid binding domain
FAD_binding_6	PF00970	656 - 762	2.1e-37	Oxidoreductase FAD-binding domain
NAD_binding_1	PF00175	782 - 888	1.1e-34	Oxidoreductase NAD-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0010866)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G37130 AT1G77760
fragaria_vesca	FvH4_5g39010
malus_domestica	MD08G1172400.v1.1 MD15G1357900.v1.1
prunus_persica	Prupe.1G505400_v2.0.a1
pyrus_communis	pycom08g14700 pycom15g32000
rosa_chinensis	RchiOBHm_Chr7g0240971
rosa_laevigata	RLG00000000608
rosa_multiflora	Rmu_co8418311.1_g000001 Rmu_sc0041887.1_g000001 Rmu_ssc0000392.1_g000001
rosa_roxburghii	Rroxscaffold_3G00219350
rosa_rugosa	Rorug07G0341100
rosa_samantha	Rh7AG495900 Rh7BG467400 Rh7CG513400 Rh7DG480400
rosa_wichuraiana	Rw7G041900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	446, 931, 965
AatII	GACGTC	1 cut(s)	657
Acc36I	ACCTGC	1 cut(s)	735
Acc65I	GGTACC	1 cut(s)	1802
AccB1I	GGYRCC	4 cut(s)	605, 692, 716, 1802
AccBSI	CCGCTC	2 cut(s)	69, 349
AccI	GTMKAC	3 cut(s)	1581, 1622, 1650
AccII	CGCG	8 cut(s)	516, 522, 548, 636, 651, 1940, 1951, 1953
AccIII	TCCGGA	2 cut(s)	216, 1142
AclWI	GGATC	4 cut(s)	680, 2372, 2614, 2644
AcoI	YGGCCR	2 cut(s)	777, 996
AcsI	RAATTY	5 cut(s)	1099, 1897, 1959, 2015, 2701
AcuI	CTGAAG	2 cut(s)	1026, 2013
AcvI	CACGTG	1 cut(s)	432
AcyI	GRCGYC	8 cut(s)	60, 414, 606, 615, 654, 693, 717, 1070
AfiI	CCNNNNNNNGG	9 cut(s)	562, 630, 737, 845, 846, 1430, 1488, 1851, 2204
AflIII	ACRYGT	1 cut(s)	702
AgsI	TTSAA	6 cut(s)	79, 334, 598, 1739, 2014, 2512
AhdI	GACNNNNNGTC	2 cut(s)	160, 1882
AjiI	CACGTC	5 cut(s)	273, 1204, 1279, 2002, 2257
AjnI	CCWGG	3 cut(s)	730, 871, 1196
AjuI	GAANNNNNNNTTGG	4 cut(s)	86, 118, 2252, 2284
AloI	GAACNNNNNNTCC	2 cut(s)	1556, 1588
Alw21I	GWGCWC	3 cut(s)	528, 1290, 2187
Alw26I	GTCTC	6 cut(s)	622, 1080, 1187, 1508, 1552, 2253
Alw44I	GTGCAC	1 cut(s)	2183
AlwI	GGATC	4 cut(s)	680, 2372, 2614, 2644
Ama87I	CYCGRG	1 cut(s)	1681
Aor13HI	TCCGGA	2 cut(s)	216, 1142
ApaI	GGGCCC	1 cut(s)	2640
ApaLI	GTGCAC	1 cut(s)	2183
ApeKI	GCWGC	1 cut(s)	2665
ApoI	RAATTY	5 cut(s)	1099, 1897, 1959, 2015, 2701
ArsI	GACNNNNNNTTYG	4 cut(s)	900, 932, 2415, 2447
Asp718I	GGTACC	1 cut(s)	1802
AsuC2I	CCSGG	5 cut(s)	481, 1470, 1682, 1683, 2408
AsuII	TTCGAA	1 cut(s)	711
AvaI	CYCGRG	1 cut(s)	1681
BaeGI	GKGCMC	2 cut(s)	2187, 2640
BalI	TGGCCA	2 cut(s)	779, 998
BanI	GGYRCC	4 cut(s)	605, 692, 716, 1802
BanII	GRGCYC	3 cut(s)	528, 2211, 2640
BauI	CACGAG	2 cut(s)	1079, 1164
BbrPI	CACGTG	1 cut(s)	432
BbsI	GAAGAC	2 cut(s)	1878, 2040
Bbv12I	GWGCWC	3 cut(s)	528, 1290, 2187
BbvI	GCAGC	1 cut(s)	2677
BccI	CCATC	4 cut(s)	886, 1486, 2402, 2597
BceAI	ACGGC	8 cut(s)	73, 226, 385, 427, 838, 1057, 1099, 2645
BciT130I	CCWGG	3 cut(s)	732, 873, 1198
BclI	TGATCA	1 cut(s)	864
BcnI	CCSGG	5 cut(s)	481, 1470, 1682, 1683, 2408
BcoDI	GTCTC	6 cut(s)	622, 1080, 1187, 1508, 1552, 2253
BfaI	CTAG	5 cut(s)	1181, 1662, 1827, 2045, 2252
BfmI	CTRYAG	1 cut(s)	676
BfoI	RGCGCY	3 cut(s)	609, 696, 720
BfuAI	ACCTGC	1 cut(s)	735
BglI	GCCNNNNNGGC	2 cut(s)	687, 995
BlpI	GCTNAGC	1 cut(s)	350
BmcAI	AGTACT	2 cut(s)	88, 2222
Bme1390I	CCNGG	8 cut(s)	481, 732, 873, 1198, 1470, 1682, 1683, 2408
BmeRI	GACNNNNNGTC	2 cut(s)	160, 1882
BmeT110I	CYCGRG	1 cut(s)	1681
BmgBI	CACGTC	5 cut(s)	273, 1204, 1279, 2002, 2257
BmrFI	CCNGG	8 cut(s)	481, 732, 873, 1198, 1470, 1682, 1683, 2408
BmrI	ACTGGG	1 cut(s)	610
BmsI	GCATC	4 cut(s)	201, 921, 976, 1465
BmuI	ACTGGG	1 cut(s)	610
BoxI	GACNNNNGTC	1 cut(s)	2040
BpiI	GAAGAC	2 cut(s)	1878, 2040
BpmI	CTGGAG	2 cut(s)	151, 2696
Bpu1102I	GCTNAGC	1 cut(s)	350
Bpu14I	TTCGAA	1 cut(s)	711
BpuEI	CTTGAG	3 cut(s)	644, 1520, 1793
BpuMI	CCSGG	5 cut(s)	481, 1470, 1682, 1683, 2408
Bsa29I	ATCGAT	1 cut(s)	144
BsaAI	YACGTR	1 cut(s)	432
BsaHI	GRCGYC	8 cut(s)	60, 414, 606, 615, 654, 693, 717, 1070
BsaI	GGTCTC	2 cut(s)	1187, 1508
BsaWI	WCCGGW	4 cut(s)	216, 382, 853, 1142
BsaXI	ACNNNNNCTCC	4 cut(s)	1076, 1106, 1814, 1844
Bsc4I	CCNNNNNNNGG	9 cut(s)	562, 630, 737, 845, 846, 1430, 1488, 1851, 2204
Bse118I	RCCGGY	3 cut(s)	354, 458, 611
Bse1I	ACTGG	9 cut(s)	28, 446, 605, 759, 1256, 1278, 1302, 2054, 2653
Bse3DI	GCAATG	1 cut(s)	2021
BseAI	TCCGGA	2 cut(s)	216, 1142
BseBI	CCWGG	3 cut(s)	732, 873, 1198
BseCI	ATCGAT	1 cut(s)	144
BseGI	GGATG	6 cut(s)	119, 897, 991, 1456, 1497, 2394
BseLI	CCNNNNNNNGG	9 cut(s)	562, 630, 737, 845, 846, 1430, 1488, 1851, 2204
BseMI	GCAATG	1 cut(s)	2021
BseMII	CTCAG	5 cut(s)	24, 364, 755, 1361, 2576
BseNI	ACTGG	9 cut(s)	28, 446, 605, 759, 1256, 1278, 1302, 2054, 2653
BseRI	GAGGAG	3 cut(s)	61, 1097, 1446
BseSI	GKGCMC	2 cut(s)	2187, 2640
BseXI	GCAGC	1 cut(s)	2677
BseYI	CCCAGC	2 cut(s)	282, 2547
BsgI	GTGCAG	2 cut(s)	1642, 2684
Bsh1236I	CGCG	8 cut(s)	516, 522, 548, 636, 651, 1940, 1951, 1953
Bsh1285I	CGRYCG	2 cut(s)	144, 591
BshNI	GGYRCC	4 cut(s)	605, 692, 716, 1802
BshVI	ATCGAT	1 cut(s)	144
BsiEI	CGRYCG	2 cut(s)	144, 591
BsiHKAI	GWGCWC	3 cut(s)	528, 1290, 2187
BsiHKCI	CYCGRG	1 cut(s)	1681
BslFI	GGGAC	4 cut(s)	371, 404, 1343, 2371
BslI	CCNNNNNNNGG	9 cut(s)	562, 630, 737, 845, 846, 1430, 1488, 1851, 2204
BsmAI	GTCTC	6 cut(s)	622, 1080, 1187, 1508, 1552, 2253
BsmBI	CGTCTC	3 cut(s)	622, 1080, 2253
BsmFI	GGGAC	4 cut(s)	371, 404, 1343, 2371
Bso31I	GGTCTC	2 cut(s)	1187, 1508
BsoBI	CYCGRG	1 cut(s)	1681
Bsp119I	TTCGAA	1 cut(s)	711
Bsp120I	GGGCCC	1 cut(s)	2636
Bsp1286I	GDGCHC	5 cut(s)	528, 1290, 2187, 2211, 2640
Bsp13I	TCCGGA	2 cut(s)	216, 1142
Bsp1720I	GCTNAGC	1 cut(s)	350
Bsp19I	CCATGG	3 cut(s)	489, 780, 1849
BspCNI	CTCAG	5 cut(s)	23, 363, 756, 1360, 2577
BspDI	ATCGAT	1 cut(s)	144
BspEI	TCCGGA	2 cut(s)	216, 1142
BspFNI	CGCG	8 cut(s)	516, 522, 548, 636, 651, 1940, 1951, 1953
BspHI	TCATGA	1 cut(s)	1560
BspMI	ACCTGC	1 cut(s)	735
BspPI	GGATC	4 cut(s)	680, 2372, 2614, 2644
BspQI	GCTCTTC	1 cut(s)	2469
BspT104I	TTCGAA	1 cut(s)	711
BspT107I	GGYRCC	4 cut(s)	605, 692, 716, 1802
BspTNI	GGTCTC	2 cut(s)	1187, 1508
BsrBI	CCGCTC	2 cut(s)	69, 349
BsrDI	GCAATG	1 cut(s)	2021
BsrFI	RCCGGY	3 cut(s)	354, 458, 611
BsrI	ACTGG	9 cut(s)	28, 446, 605, 759, 1256, 1278, 1302, 2054, 2653
BssAI	RCCGGY	3 cut(s)	354, 458, 611
BssNI	GRCGYC	8 cut(s)	60, 414, 606, 615, 654, 693, 717, 1070
BssSI	CACGAG	2 cut(s)	1079, 1164
BssT1I	CCWWGG	7 cut(s)	423, 489, 780, 1305, 1575, 1849, 2038
Bst2BI	CACGAG	2 cut(s)	1079, 1164
Bst2UI	CCWGG	3 cut(s)	732, 873, 1198
Bst6I	CTCTTC	5 cut(s)	1535, 1555, 1725, 2448, 2469
BstACI	GRCGYC	8 cut(s)	60, 414, 606, 615, 654, 693, 717, 1070
BstAPI	GCANNNNNTGC	1 cut(s)	2100
BstBAI	YACGTR	1 cut(s)	432
BstBI	TTCGAA	1 cut(s)	711
BstDEI	CTNAG	5 cut(s)	10, 350, 764, 1347, 2585
BstDSI	CCRYGG	7 cut(s)	367, 409, 489, 780, 1639, 1849, 2311
BstEII	GGTNACC	1 cut(s)	454
BstF5I	GGATG	6 cut(s)	119, 897, 991, 1456, 1497, 2394
BstFNI	CGCG	8 cut(s)	516, 522, 548, 636, 651, 1940, 1951, 1953
BstH2I	RGCGCY	3 cut(s)	609, 696, 720
BstMAI	GTCTC	6 cut(s)	622, 1080, 1187, 1508, 1552, 2253
BstMCI	CGRYCG	2 cut(s)	144, 591
BstNI	CCWGG	3 cut(s)	732, 873, 1198
BstNSI	RCATGY	4 cut(s)	817, 983, 2432, 2597
BstPAI	GACNNNNGTC	1 cut(s)	2040
BstPI	GGTNACC	1 cut(s)	454
BstSCI	CCNGG	8 cut(s)	479, 730, 871, 1196, 1468, 1680, 1681, 2406
BstSFI	CTRYAG	1 cut(s)	676
BstSLI	GKGCMC	2 cut(s)	2187, 2640
BstUI	CGCG	8 cut(s)	516, 522, 548, 636, 651, 1940, 1951, 1953
BstV1I	GCAGC	1 cut(s)	2677
BstV2I	GAAGAC	2 cut(s)	1878, 2040
BstX2I	RGATCY	1 cut(s)	672
BstYI	RGATCY	1 cut(s)	672
Bsu15I	ATCGAT	1 cut(s)	144
BsuTUI	ATCGAT	1 cut(s)	144
BtgI	CCRYGG	7 cut(s)	367, 409, 489, 780, 1639, 1849, 2311
BtrI	CACGTC	5 cut(s)	273, 1204, 1279, 2002, 2257
BtsCI	GGATG	6 cut(s)	119, 897, 991, 1456, 1497, 2394
BtsIMutI	CAGTG	3 cut(s)	1271, 2061, 2563
BveI	ACCTGC	1 cut(s)	735
CciI	TCATGA	1 cut(s)	1560
Cfr10I	RCCGGY	3 cut(s)	354, 458, 611
Cfr9I	CCCGGG	1 cut(s)	1681
ClaI	ATCGAT	1 cut(s)	144
CseI	GACGC	6 cut(s)	49, 403, 604, 1078, 1515, 1924
DdeI	CTNAG	5 cut(s)	10, 350, 764, 1347, 2585
DinI	GGCGCC	3 cut(s)	607, 694, 718
DrdI	GACNNNNNNGTC	3 cut(s)	446, 931, 965
DriI	GACNNNNNGTC	2 cut(s)	160, 1882
DseDI	GACNNNNNNGTC	3 cut(s)	446, 931, 965
EaeI	YGGCCR	2 cut(s)	777, 996
Eam1104I	CTCTTC	5 cut(s)	1535, 1555, 1725, 2448, 2469
Eam1105I	GACNNNNNGTC	2 cut(s)	160, 1882
EarI	CTCTTC	5 cut(s)	1535, 1555, 1725, 2448, 2469
EciI	GGCGGA	2 cut(s)	299, 904
Ecl136II	GAGCTC	1 cut(s)	526
Eco130I	CCWWGG	7 cut(s)	423, 489, 780, 1305, 1575, 1849, 2038
Eco24I	GRGCYC	3 cut(s)	528, 2211, 2640
Eco31I	GGTCTC	2 cut(s)	1187, 1508
Eco32I	GATATC	1 cut(s)	799
Eco53kI	GAGCTC	1 cut(s)	526
Eco57I	CTGAAG	2 cut(s)	1026, 2013
Eco72I	CACGTG	1 cut(s)	432
Eco88I	CYCGRG	1 cut(s)	1681
Eco91I	GGTNACC	1 cut(s)	454
EcoICRI	GAGCTC	1 cut(s)	526
EcoO109I	RGGNCCY	3 cut(s)	724, 967, 2637
EcoO65I	GGTNACC	1 cut(s)	454
EcoRI	GAATTC	1 cut(s)	2701
EcoRII	CCWGG	3 cut(s)	730, 871, 1196
EcoRV	GATATC	1 cut(s)	799
EcoT14I	CCWWGG	7 cut(s)	423, 489, 780, 1305, 1575, 1849, 2038
EcoT22I	ATGCAT	1 cut(s)	2430
EcoT38I	GRGCYC	3 cut(s)	528, 2211, 2640
EgeI	GGCGCC	3 cut(s)	607, 694, 718
EheI	GGCGCC	3 cut(s)	607, 694, 718
ErhI	CCWWGG	7 cut(s)	423, 489, 780, 1305, 1575, 1849, 2038
Esp3I	CGTCTC	3 cut(s)	622, 1080, 2253
FalI	AAGNNNNNCTT	2 cut(s)	2156, 2188
FaqI	GGGAC	4 cut(s)	371, 404, 1343, 2371
FauI	CCCGC	8 cut(s)	55, 484, 527, 541, 662, 1478, 1623, 2627
FbaI	TGATCA	1 cut(s)	864
FblI	GTMKAC	3 cut(s)	1581, 1622, 1650
FokI	GGATG	6 cut(s)	106, 904, 998, 1443, 1504, 2381
FriOI	GRGCYC	3 cut(s)	528, 2211, 2640
FspBI	CTAG	5 cut(s)	1181, 1662, 1827, 2045, 2252
GsaI	CCCAGC	2 cut(s)	286, 2551
GsuI	CTGGAG	2 cut(s)	151, 2696
HaeII	RGCGCY	3 cut(s)	609, 696, 720
HgaI	GACGC	6 cut(s)	49, 403, 604, 1078, 1515, 1924
Hin1I	GRCGYC	8 cut(s)	60, 414, 606, 615, 654, 693, 717, 1070
HindIII	AAGCTT	2 cut(s)	1970, 2096
HpyAV	CCTTC	8 cut(s)	340, 707, 922, 980, 1567, 2037, 2254, 2537
HpyCH4IV	ACGT	9 cut(s)	272, 399, 431, 654, 702, 1203, 1278, 2001, 2256
HpyF3I	CTNAG	5 cut(s)	10, 350, 764, 1347, 2585
HpySE526I	ACGT	9 cut(s)	272, 399, 431, 654, 702, 1203, 1278, 2001, 2256
Hsp92I	GRCGYC	8 cut(s)	60, 414, 606, 615, 654, 693, 717, 1070
KasI	GGCGCC	3 cut(s)	605, 692, 716
Kpn2I	TCCGGA	2 cut(s)	216, 1142
KpnI	GGTACC	1 cut(s)	1806
KroI	GCCGGC	2 cut(s)	354, 611
KroNI	GCCGGC	2 cut(s)	356, 613
Ksp22I	TGATCA	1 cut(s)	864
LguI	GCTCTTC	1 cut(s)	2469
LmnI	GCTCC	8 cut(s)	74, 289, 340, 531, 565, 1835, 1912, 2590
Lsp1109I	GCAGC	1 cut(s)	2677
LweI	GCATC	4 cut(s)	201, 921, 976, 1465
MaeI	CTAG	5 cut(s)	1181, 1662, 1827, 2045, 2252
MaeII	ACGT	9 cut(s)	272, 399, 431, 654, 702, 1203, 1278, 2001, 2256
MbiI	CCGCTC	2 cut(s)	69, 349
MboII	GAAGA	8 cut(s)	1542, 1552, 1742, 1878, 2045, 2465, 2486, 2692
MfeI	CAATTG	2 cut(s)	2085, 2238
MflI	RGATCY	1 cut(s)	672
MhlI	GDGCHC	5 cut(s)	528, 1290, 2187, 2211, 2640
MlsI	TGGCCA	2 cut(s)	779, 998
MluCI	AATT	8 cut(s)	233, 1099, 1897, 1959, 2015, 2085, 2238, 2701
MluNI	TGGCCA	2 cut(s)	779, 998
Mly113I	GGCGCC	3 cut(s)	606, 693, 717
MlyI	GAGTC	5 cut(s)	941, 1171, 1817, 1871, 2222
MmeI	TCCRAC	5 cut(s)	255, 915, 1484, 1548, 1776
Mox20I	TGGCCA	2 cut(s)	779, 998
Mph1103I	ATGCAT	1 cut(s)	2430
MreI	CGCCGGCG	1 cut(s)	611
MroI	TCCGGA	2 cut(s)	216, 1142
MroNI	GCCGGC	2 cut(s)	354, 611
MscI	TGGCCA	2 cut(s)	779, 998
MseI	TTAA	2 cut(s)	1703, 2175
MslI	CAYNNNNRTG	5 cut(s)	917, 984, 1379, 1644, 2423
Msp20I	TGGCCA	2 cut(s)	779, 998
MspA1I	CMGCKG	1 cut(s)	282
MspR9I	CCNGG	8 cut(s)	481, 732, 873, 1198, 1470, 1682, 1683, 2408
MunI	CAATTG	2 cut(s)	2085, 2238
MvaI	CCWGG	3 cut(s)	732, 873, 1198
MvnI	CGCG	8 cut(s)	516, 522, 548, 636, 651, 1940, 1951, 1953
NaeI	GCCGGC	2 cut(s)	356, 613
NarI	GGCGCC	3 cut(s)	606, 693, 717
NciI	CCSGG	5 cut(s)	481, 1470, 1682, 1683, 2408
NcoI	CCATGG	3 cut(s)	489, 780, 1849
NgoMIV	GCCGGC	2 cut(s)	354, 611
NmeAIII	GCCGAG	3 cut(s)	113, 745, 1015
NmuCI	GTSAC	9 cut(s)	454, 532, 919, 1159, 1174, 1269, 1975, 2214, 2273
NsiI	ATGCAT	1 cut(s)	2430
NspI	RCATGY	4 cut(s)	817, 983, 2432, 2597
NspV	TTCGAA	1 cut(s)	711
PagI	TCATGA	1 cut(s)	1560
PciSI	GCTCTTC	1 cut(s)	2469
PcsI	WCGNNNNNNNCGW	9 cut(s)	150, 180, 183, 192, 444, 708, 717, 1064, 1100
PdiI	GCCGGC	2 cut(s)	356, 613
PfeI	GAWTC	5 cut(s)	158, 594, 1520, 1665, 2565
PflFI	GACNNNGTC	1 cut(s)	1072
Ple19I	CGATCG	2 cut(s)	144, 591
PleI	GAGTC	5 cut(s)	940, 1171, 1817, 1871, 2222
PluTI	GGCGCC	3 cut(s)	609, 696, 720
PmaCI	CACGTG	1 cut(s)	432
PmlI	CACGTG	1 cut(s)	432
PpsI	GAGTC	5 cut(s)	940, 1171, 1817, 1871, 2222
Ppu21I	YACGTR	1 cut(s)	432
PpuMI	RGGWCCY	2 cut(s)	724, 967
PshAI	GACNNNNGTC	1 cut(s)	2040
Psp124BI	GAGCTC	1 cut(s)	528
Psp5II	RGGWCCY	2 cut(s)	724, 967
Psp6I	CCWGG	3 cut(s)	730, 871, 1196
PspCI	CACGTG	1 cut(s)	432
PspEI	GGTNACC	1 cut(s)	454
PspFI	CCCAGC	2 cut(s)	282, 2547
PspGI	CCWGG	3 cut(s)	730, 871, 1196
PspOMI	GGGCCC	1 cut(s)	2636
PspPPI	RGGWCCY	2 cut(s)	724, 967
PsuI	RGATCY	1 cut(s)	672
PsyI	GACNNNGTC	1 cut(s)	1072
PvuI	CGATCG	2 cut(s)	144, 591
PvuII	CAGCTG	1 cut(s)	282
RseI	CAYNNNNRTG	5 cut(s)	917, 984, 1379, 1644, 2423
SacI	GAGCTC	1 cut(s)	528
SapI	GCTCTTC	1 cut(s)	2469
SaqAI	TTAA	2 cut(s)	1703, 2175
ScaI	AGTACT	2 cut(s)	88, 2222
SchI	GAGTC	5 cut(s)	941, 1171, 1817, 1871, 2222
ScrFI	CCNGG	8 cut(s)	481, 732, 873, 1198, 1470, 1682, 1683, 2408
SduI	GDGCHC	5 cut(s)	528, 1290, 2187, 2211, 2640
SfaNI	GCATC	4 cut(s)	201, 921, 976, 1465
SfcI	CTRYAG	1 cut(s)	676
SfoI	GGCGCC	3 cut(s)	607, 694, 718
SfuI	TTCGAA	1 cut(s)	711
SgrAI	CRCCGGYG	1 cut(s)	611
SmaI	CCCGGG	1 cut(s)	1683
SmiMI	CAYNNNNRTG	5 cut(s)	917, 984, 1379, 1644, 2423
SmlI	CTYRAG	3 cut(s)	659, 1535, 1772
SmoI	CTYRAG	3 cut(s)	659, 1535, 1772
Sse9I	AATT	8 cut(s)	233, 1099, 1897, 1959, 2015, 2085, 2238, 2701
SspDI	GGCGCC	3 cut(s)	605, 692, 716
SspMI	CTAG	5 cut(s)	1181, 1662, 1827, 2045, 2252
SstI	GAGCTC	1 cut(s)	528
StyD4I	CCNGG	8 cut(s)	479, 730, 871, 1196, 1468, 1680, 1681, 2406
StyI	CCWWGG	7 cut(s)	423, 489, 780, 1305, 1575, 1849, 2038
TaiI	ACGT	9 cut(s)	275, 402, 434, 657, 705, 1206, 1281, 2004, 2259
TaqI	TCGA	8 cut(s)	42, 144, 245, 450, 711, 1094, 1988, 2129
TaqII	GACCGA	2 cut(s)	1048, 1532
TasI	AATT	8 cut(s)	233, 1099, 1897, 1959, 2015, 2085, 2238, 2701
TatI	WGTACW	6 cut(s)	86, 201, 1029, 2169, 2220, 2283
TauI	GCSGC	9 cut(s)	8, 346, 349, 475, 561, 611, 639, 684, 693
TfiI	GAWTC	5 cut(s)	158, 594, 1520, 1665, 2565
Tru1I	TTAA	2 cut(s)	1703, 2175
Tru9I	TTAA	2 cut(s)	1703, 2175
TscAI	CASTG	3 cut(s)	1278, 2061, 2563
TseFI	GTSAC	9 cut(s)	454, 532, 919, 1159, 1174, 1269, 1975, 2214, 2273
TseI	GCWGC	1 cut(s)	2665
Tsp45I	GTSAC	9 cut(s)	454, 532, 919, 1159, 1174, 1269, 1975, 2214, 2273
TspDTI	ATGAA	8 cut(s)	364, 1400, 1549, 1577, 2151, 2517, 2557, 2699
TspGWI	ACGGA	2 cut(s)	616, 2588
TspMI	CCCGGG	1 cut(s)	1681
TspRI	CASTG	3 cut(s)	1278, 2061, 2563
Tth111I	GACNNNGTC	1 cut(s)	1072
VneI	GTGCAC	1 cut(s)	2183
XapI	RAATTY	5 cut(s)	1099, 1897, 1959, 2015, 2701
XbaI	TCTAGA	2 cut(s)	1180, 2251
XceI	RCATGY	4 cut(s)	817, 983, 2432, 2597
XmaI	CCCGGG	1 cut(s)	1681
XmiI	GTMKAC	3 cut(s)	1581, 1622, 1650
XspI	CTAG	5 cut(s)	1181, 1662, 1827, 2045, 2252
ZraI	GACGTC	1 cut(s)	655
ZrmI	AGTACT	2 cut(s)	88, 2222
Zsp2I	ATGCAT	1 cut(s)	2430

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.