Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0010866

Physical Location & Seq

Genomic Coordinates Reverse (-)

43153160 .. 43158286

Gene Structure 5127 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1357900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2712 bp

ATGACGGCCTCCGTTGAGAACCGCCAGTTCCCCCACCTCGACCCGCCGATAAACGGCGTCGTCCGCTCCTTCAACTTCTCCGATCTCACTCCCTCGAAACAAGCGCCCTTCGTCGATAGCAACGACTCATCGTCCGATGACGACGAGAATGAGGACACCATCGTCGAGGAATACACGGATTTAATCCGGAGAGGCAACAACGAATTAGAGCCGTCCATCTTGGACCCCAGAGATGAAGCCACGTCAGACAGCTGGGTGCGTCGTAACTCGTCTATGGTCCGCCTCACTGGAAAGCACCCCTTCAATTGCGAACCGCCACTCACCCGCCTCATGCACCACGGATTCATCACCCCAGTCCCACTCCACTACGTCCGCAATCACGGCATCGTTCCCAAGGCCACGTGGCAGGACTGGACCGTCGAGATAACCGGTCTGGTTCGCCGCCCGACCCGGTTCACCATGGACCAGCTCGTCCGCGAATTCAAGCCACGGGAGTTCCCCGTGACCCTTGTCTGCGCGGGAAACCGGAGGAAAGAACAGAATCTCGTCAAGCAGACAATCGGCTTCAACTGGGGCGCCGCCGGCATCTCCAACTCTGTGTGGCGCGGCATCCCGCTTTGCGAAGTGCTGAAGCGGTGCGGAATCCGCAGCCGCAAGAACGGCGCCCTCAACGTGTGCTTCGAGGGAGCCGAGGACCTGCCGGGTGGCGGTGGGACCAAGTACGGCACGAGCTTGAGAAAGGAGGTGGCGATGGACCCCTCGAGGGATATAATCCTGGCGTACATGCAAAACGGGGAGCCTTTGGCGCCGGATCACGGGTTTCCGGTTCGGGTCATAATACCGGGGTTCATCGGGGGTCGGATGGTGAAGTGGTTGAAACGAATCGTGGTCACAACCTCGGAATCCGACAATTATTACCACTTCAAGGATAATCGAGTGCTCCCGTCGCACGTGGATGCGGAGCTGGCCAACGCTGAAGCGTGGTGGTACAAGCCGGAGTATATCATTAACGAGCTGAATATAAACTCGGTTATAACGACGCCGTGTCACGAGGAGATTTTGCCGATCAACTCGTATACGACTCAACATCCGTACACGATTAAAGGCTATGCATATTCGGGCGGCGGGAAGAAAGTGACACGAGTGGAGATAACAATGGACGGCGGGGACACGTGGCATGTCTGCACGTTAGACCACCAGGAGAGGCCAAACAAATATGGGAAGTACTGGTGCTGGTGTTTCTGGTCACTGGAAGTGGAGGTGCTCGACCTACTTGCTGCCAAAGAGATCGCCGTCCGAGCTTGGGACGAGACTCTCAATACTCAGCCCGAGAAACTCATTTGGAACGTCATGGGAATGATGAACAACTGCTGGTTTCGAGTGAAAACCAATGTGTGCAAACCACACAAGGGAGAGATAGGACTAGCGTTTGAGCATCCAACTCAACCTGGAAACCAATCAGGTGGGTGGATGGCCAAAGAAAAACGCTTGGACTCTGAATCCAACACAACCCTAAAGAAGAGTTTTTCAACACCCTTCATGAACACTAGTGCATCCAAGATGTACTCAATGTCAGATGTCAAGAAGCACAACTCTTCTCAATCTGCTTGGATCGTCGTCCACGGCCACGTCTACGACTGCACTCGCTTCCTCAATGATCACCCTGGTGGCGCTGATAGCATTCTCATCAACGCTGGCACTGATTGCACCGAAGAGTTTGATGCCATCCATTCGGAAAAAGCCAAGAAAATGATTGAGGATTATAGAATCGGAGAGTTGGTGACTACGAATTATGCTTCAGACTCCACATCTAGTTCCCCTAATAACTCTGTACATGGGCCACCAAGAACAACATCTGTACTTGACCTTATTTCTTCTAACATGACGCAGCTAGCCCCAATCAAAGAAGTAATGCATTCAAGAAGCATCGCACTTATTCCGCGTGAGAAAATCCCATGTAAGCTCATAGCCAAGATATCGATCTCTCACGACGTCAGGCTCTTTAGATTCGGATTGCCTTCGGAGGACCAAGTTTTGGGATTGCCGGTGGGAAAGCACATTTTCGTGTGTGCTACAATTGAGGATAAGCTTTGCATGAGGGCTTACACTCCAAGCAGCTCAATTGATGAAGTTGGGTACTTTGATTTGGTGGTGAAAGTTTACTTCAAGAATGTGCATCCTAAGTTCCCCAACGGGGGTGTCATGTCACAGTATTTGGACTCACTGCCAATAGGTGCATCCCTAGACATCAAGGGTCCCTTAGGGCACATAGAGTATACTGGTCGCGGTAATTTTATGGTCAACGGCAAGCCAAAGTTTGCAAAGAAGCTTGCCATGCTGGCTGGTGGGACCGGGATCACCCCAATTTACCAAGTGGTTCAAGCCATATTGAAGGACCCAGAGGATAAGACCGAGATGTATGTGGTGTATGCAAACCGTACAGAAGATGACATTTTGCTGCGGGAAGAACTTGATGGTTGGGCCAAACAACATGAGAGGTTCAAGGTTTGGTATGTGGTGGAGAATGGCAGGGAAGGGTGGGAATACAGTGTGGGATTCGTCACGGAGAGTGTGCTCAGGGAGCACATTCCCGATGGATCGGACGGCGCTCTTGCGCTGGCATGCGGTCCCCCGCCGATGATCAAATTTGCCGTGCAGCCGAATTTGGAGAAGATGAATTATGATACCACAAATTCATTGCTGTTGTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001505 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0005886 GO:0006082 GO:0006807 GO:0006809 GO:0008150 GO:0008152 GO:0008940 GO:0009058 GO:0009314 GO:0009416 GO:0009605 GO:0009607 GO:0009608 GO:0009610 GO:0009620 GO:0009628 GO:0009703 GO:0009987 GO:0016020 GO:0016491 GO:0016661 GO:0017144 GO:0034641 GO:0042126 GO:0042128 GO:0042133 GO:0042136 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046209 GO:0046857 GO:0050896 GO:0051704 GO:0051707 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:0071941 GO:0071944 GO:0072593 GO:1903409 GO:2001057

KEGG Pathways

Metabolic & Signaling

ko00910 ko01120 map00910 map01120

Pfam Domains

Protein Families

Cyt-b5 FAD_binding_6 Mo-co_dimer NAD_binding_1 Oxidored_molyb

Protein Analysis

904

Amino Acids

101.63

Weight (kDa)

6.3

Isoelectric Point (pI)

41.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Oxidored_molyb	PF00174	127 - 306	1.9e-55	Oxidoreductase molybdopterin binding domain
Mo-co_dimer	PF03404	334 - 465	2.3e-56	Mo-co oxidoreductase dimerisation domain
Cyt-b5	PF00173	524 - 593	4.5e-27	Cytochrome b5-like Heme/Steroid binding domain
FAD_binding_6	PF00970	653 - 759	1.3e-36	Oxidoreductase FAD-binding domain
NAD_binding_1	PF00175	779 - 885	1.3e-35	Oxidoreductase NAD-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0010866)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G37130 AT1G77760
fragaria_vesca	FvH4_5g39010
malus_domestica	MD08G1172400.v1.1 MD15G1357900.v1.1
prunus_persica	Prupe.1G505400_v2.0.a1
pyrus_communis	pycom08g14700 pycom15g32000
rosa_chinensis	RchiOBHm_Chr7g0240971
rosa_laevigata	RLG00000000608
rosa_multiflora	Rmu_co8418311.1_g000001 Rmu_sc0041887.1_g000001 Rmu_ssc0000392.1_g000001
rosa_roxburghii	Rroxscaffold_3G00219350
rosa_rugosa	Rorug07G0341100
rosa_samantha	Rh7AG495900 Rh7BG467400 Rh7CG513400 Rh7DG480400
rosa_wichuraiana	Rw7G041900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1034
AasI	GACNNNNNNGTC	3 cut(s)	161, 416, 470
AatII	GACGTC	1 cut(s)	1995
AbsI	CCTCGAGG	1 cut(s)	760
Acc36I	ACCTGC	1 cut(s)	705
AccB1I	GGYRCC	3 cut(s)	575, 662, 805
AccB7I	CCANNNNNTGG	1 cut(s)	2035
AccBSI	CCGCTC	1 cut(s)	66
AccI	GTMKAC	3 cut(s)	1076, 1632, 2276
AccII	CGCG	5 cut(s)	477, 518, 606, 1942, 2286
AccIII	TCCGGA	1 cut(s)	186
AclWI	GGATC	4 cut(s)	819, 1619, 2363, 2605
AcoI	YGGCCR	3 cut(s)	966, 1473, 1624
AcsI	RAATTY	4 cut(s)	479, 2645, 2662, 2692
AcuI	CTGAAG	3 cut(s)	650, 996, 1782
AcvI	CACGTG	3 cut(s)	402, 952, 1173
AcyI	GRCGYC	6 cut(s)	57, 576, 663, 806, 1040, 1992
AflIII	ACRYGT	2 cut(s)	672, 1170
AgeI	ACCGGT	1 cut(s)	428
AhdI	GACNNNNNGTC	2 cut(s)	130, 1044
AhlI	ACTAGT	1 cut(s)	1547
AjiI	CACGTC	2 cut(s)	243, 1630
AjnI	CCWGG	4 cut(s)	774, 1197, 1447, 1663
AleI	CACNNNNGTG	1 cut(s)	1665
Alw21I	GWGCWC	4 cut(s)	942, 1266, 2577, 2586
Alw26I	GTCTC	1 cut(s)	1304
AlwI	GGATC	4 cut(s)	819, 1619, 2363, 2605
Ama87I	CYCGRG	2 cut(s)	760, 1328
Aor13HI	TCCGGA	1 cut(s)	186
AoxI	GGCC	8 cut(s)	6, 396, 966, 1205, 1473, 1624, 1838, 2481
ApeKI	GCWGC	6 cut(s)	648, 1277, 1888, 2115, 2458, 2656
ApoI	RAATTY	4 cut(s)	479, 2645, 2662, 2692
AsiGI	ACCGGT	1 cut(s)	428
AspLEI	GCGC	9 cut(s)	106, 518, 578, 606, 665, 808, 1673, 2609, 2617
AsuC2I	CCSGG	4 cut(s)	451, 702, 843, 2353
AsuHPI	GGTGA	8 cut(s)	313, 340, 448, 877, 1652, 1792, 2164, 2350
AsuNHI	GCTAGC	1 cut(s)	1891
AvaI	CYCGRG	2 cut(s)	760, 1328
AxyI	CCTNAGG	1 cut(s)	2260
BaeGI	GKGCMC	1 cut(s)	2268
BalI	TGGCCA	2 cut(s)	968, 1475
BanI	GGYRCC	3 cut(s)	575, 662, 805
BauI	CACGAG	3 cut(s)	727, 1049, 1140
BbrPI	CACGTG	3 cut(s)	402, 952, 1173
Bbv12I	GWGCWC	4 cut(s)	942, 1266, 2577, 2586
BbvI	GCAGC	6 cut(s)	660, 1264, 1900, 2127, 2445, 2668
BccI	CCATC	8 cut(s)	167, 224, 745, 856, 1465, 1733, 2468, 2588
BcgI	CGANNNNNNTGC	2 cut(s)	1256, 1290
BciT130I	CCWGG	4 cut(s)	776, 1199, 1449, 1665
BclI	TGATCA	2 cut(s)	1657, 2640
BcnI	CCSGG	4 cut(s)	451, 702, 843, 2353
BcoDI	GTCTC	1 cut(s)	1304
BcuI	ACTAGT	1 cut(s)	1547
BfaI	CTAG	5 cut(s)	1424, 1548, 1812, 1892, 2243
BfoI	RGCGCY	6 cut(s)	107, 579, 666, 809, 1674, 2610
BfuAI	ACCTGC	1 cut(s)	705
BglI	GCCNNNNNGGC	1 cut(s)	2339
BmcAI	AGTACT	1 cut(s)	1226
Bme1390I	CCNGG	8 cut(s)	451, 702, 776, 843, 1199, 1449, 1665, 2353
BmeRI	GACNNNNNGTC	2 cut(s)	130, 1044
BmeT110I	CYCGRG	2 cut(s)	760, 1328
BmgBI	CACGTC	2 cut(s)	243, 1630
BmrFI	CCNGG	8 cut(s)	451, 702, 776, 843, 1199, 1449, 1665, 2353
BmrI	ACTGGG	2 cut(s)	347, 580
BmtI	GCTAGC	1 cut(s)	1895
BmuI	ACTGGG	2 cut(s)	347, 580
BoxI	GACNNNNGTC	1 cut(s)	509
Bpu10I	CCTNAGC	1 cut(s)	2576
BpuEI	CTTGAG	1 cut(s)	754
BpuMI	CCSGG	4 cut(s)	451, 702, 843, 2353
Bsa29I	ATCGAT	1 cut(s)	1979
BsaAI	YACGTR	3 cut(s)	402, 952, 1173
BsaBI	GATNNNNATC	1 cut(s)	1979
BsaHI	GRCGYC	6 cut(s)	57, 576, 663, 806, 1040, 1992
BsaJI	CCNNGG	9 cut(s)	337, 393, 459, 488, 690, 842, 897, 1621, 1663
BsaWI	WCCGGW	4 cut(s)	186, 428, 525, 823
BsaXI	ACNNNNNCTCC	2 cut(s)	1764, 1794
Bse118I	RCCGGY	3 cut(s)	428, 581, 2044
Bse1I	ACTGG	8 cut(s)	25, 292, 353, 416, 575, 1232, 1254, 2284
Bse21I	CCTNAGG	1 cut(s)	2260
Bse3DI	GCAATG	1 cut(s)	2696
Bse8I	GATNNNNATC	1 cut(s)	1979
BseAI	TCCGGA	1 cut(s)	186
BseBI	CCWGG	4 cut(s)	776, 1199, 1449, 1665
BseCI	ATCGAT	1 cut(s)	1979
BseDI	CCNNGG	9 cut(s)	337, 393, 459, 488, 690, 842, 897, 1621, 1663
BseJI	GATNNNNATC	1 cut(s)	1979
BseMI	GCAATG	1 cut(s)	2696
BseMII	CTCAG	2 cut(s)	1337, 2590
BseNI	ACTGG	8 cut(s)	25, 292, 353, 416, 575, 1232, 1254, 2284
BseRI	GAGGAG	1 cut(s)	1067
BseSI	GKGCMC	1 cut(s)	2268
BseXI	GCAGC	6 cut(s)	660, 1264, 1900, 2127, 2445, 2668
BseYI	CCCAGC	1 cut(s)	252
BsgI	GTGCAG	3 cut(s)	1168, 1624, 2675
Bsh1236I	CGCG	5 cut(s)	477, 518, 606, 1942, 2286
BshFI	GGCC	8 cut(s)	8, 398, 968, 1207, 1475, 1626, 1840, 2483
BshNI	GGYRCC	3 cut(s)	575, 662, 805
BshTI	ACCGGT	1 cut(s)	428
BshVI	ATCGAT	1 cut(s)	1979
BsiHKAI	GWGCWC	4 cut(s)	942, 1266, 2577, 2586
BsiHKCI	CYCGRG	2 cut(s)	760, 1328
BslFI	GGGAC	7 cut(s)	341, 727, 1181, 1319, 2241, 2362, 2613
BsmAI	GTCTC	1 cut(s)	1304
BsmFI	GGGAC	7 cut(s)	341, 727, 1181, 1319, 2241, 2362, 2613
BsmI	GAATGC	2 cut(s)	1680, 1915
BsnI	GGCC	8 cut(s)	8, 398, 968, 1207, 1475, 1626, 1840, 2483
BsoBI	CYCGRG	2 cut(s)	760, 1328
Bsp1286I	GDGCHC	5 cut(s)	942, 1266, 2268, 2577, 2586
Bsp13I	TCCGGA	1 cut(s)	186
Bsp1407I	TGTACA	1 cut(s)	1831
Bsp19I	CCATGG	1 cut(s)	459
BspANI	GGCC	8 cut(s)	8, 398, 968, 1207, 1475, 1626, 1840, 2483
BspCNI	CTCAG	2 cut(s)	1336, 2589
BspDI	ATCGAT	1 cut(s)	1979
BspEI	TCCGGA	1 cut(s)	186
BspFNI	CGCG	5 cut(s)	477, 518, 606, 1942, 2286
BspHI	TCATGA	1 cut(s)	1539
BspMI	ACCTGC	1 cut(s)	705
BspOI	GCTAGC	1 cut(s)	1895
BspPI	GGATC	4 cut(s)	819, 1619, 2363, 2605
BspT107I	GGYRCC	3 cut(s)	575, 662, 805
BsrBI	CCGCTC	1 cut(s)	66
BsrDI	GCAATG	1 cut(s)	2696
BsrFI	RCCGGY	3 cut(s)	428, 581, 2044
BsrGI	TGTACA	1 cut(s)	1831
BsrI	ACTGG	8 cut(s)	25, 292, 353, 416, 575, 1232, 1254, 2284
BssAI	RCCGGY	3 cut(s)	428, 581, 2044
BssECI	CCNNGG	9 cut(s)	337, 393, 459, 488, 690, 842, 897, 1621, 1663
BssNAI	GTATAC	2 cut(s)	1077, 2277
BssNI	GRCGYC	6 cut(s)	57, 576, 663, 806, 1040, 1992
BssSI	CACGAG	3 cut(s)	727, 1049, 1140
BssT1I	CCWWGG	2 cut(s)	393, 459
Bst1107I	GTATAC	2 cut(s)	1077, 2277
Bst2BI	CACGAG	3 cut(s)	727, 1049, 1140
Bst2UI	CCWGG	4 cut(s)	776, 1199, 1449, 1665
Bst4CI	ACNGT	4 cut(s)	418, 2211, 2438, 2549
Bst6I	CTCTTC	3 cut(s)	1514, 1600, 1707
BstACI	GRCGYC	6 cut(s)	57, 576, 663, 806, 1040, 1992
BstAPI	GCANNNNNTGC	1 cut(s)	1704
BstAUI	TGTACA	1 cut(s)	1831
BstBAI	YACGTR	3 cut(s)	402, 952, 1173
BstC8I	GCNNGC	9 cut(s)	583, 966, 1696, 1893, 2309, 2331, 2340, 2619, 2623
BstDEI	CTNAG	4 cut(s)	1323, 2181, 2260, 2576
BstDSI	CCRYGG	4 cut(s)	337, 459, 488, 1621
BstFNI	CGCG	5 cut(s)	477, 518, 606, 1942, 2286
BstH2I	RGCGCY	6 cut(s)	107, 579, 666, 809, 1674, 2610
BstHHI	GCGC	9 cut(s)	106, 518, 578, 606, 665, 808, 1673, 2609, 2617
BstMAI	GTCTC	1 cut(s)	1304
BstNI	CCWGG	4 cut(s)	776, 1199, 1449, 1665
BstNSI	RCATGY	3 cut(s)	787, 1181, 2625
BstPAI	GACNNNNGTC	1 cut(s)	509
BstSCI	CCNGG	8 cut(s)	449, 700, 774, 841, 1197, 1447, 1663, 2351
BstSLI	GKGCMC	1 cut(s)	2268
BstUI	CGCG	5 cut(s)	477, 518, 606, 1942, 2286
BstV1I	GCAGC	6 cut(s)	660, 1264, 1900, 2127, 2445, 2668
BstXI	CCANNNNNNTGG	1 cut(s)	1463
BstZ17I	GTATAC	2 cut(s)	1077, 2277
Bsu15I	ATCGAT	1 cut(s)	1979
Bsu36I	CCTNAGG	1 cut(s)	2260
BsuRI	GGCC	8 cut(s)	8, 398, 968, 1207, 1475, 1626, 1840, 2483
BsuTUI	ATCGAT	1 cut(s)	1979
BtgI	CCRYGG	4 cut(s)	337, 459, 488, 1621
BtgZI	GCGATG	2 cut(s)	764, 1912
BtrI	CACGTC	2 cut(s)	243, 1630
BtsI	GCAGTG	1 cut(s)	2222
BtsIMutI	CAGTG	5 cut(s)	285, 1247, 1698, 2222, 2554
BveI	ACCTGC	1 cut(s)	705
Cac8I	GCNNGC	9 cut(s)	583, 966, 1696, 1893, 2309, 2331, 2340, 2619, 2623
CciI	TCATGA	1 cut(s)	1539
CfoI	GCGC	9 cut(s)	106, 518, 578, 606, 665, 808, 1673, 2609, 2617
Cfr10I	RCCGGY	3 cut(s)	428, 581, 2044
ClaI	ATCGAT	1 cut(s)	1979
CseI	GACGC	4 cut(s)	46, 248, 1048, 1894
CspAI	ACCGGT	1 cut(s)	428
DdeI	CTNAG	4 cut(s)	1323, 2181, 2260, 2576
DinI	GGCGCC	3 cut(s)	577, 664, 807
DrdI	GACNNNNNNGTC	3 cut(s)	161, 416, 470
DriI	GACNNNNNGTC	2 cut(s)	130, 1044
DseDI	GACNNNNNNGTC	3 cut(s)	161, 416, 470
EaeI	YGGCCR	3 cut(s)	966, 1473, 1624
Eam1104I	CTCTTC	3 cut(s)	1514, 1600, 1707
Eam1105I	GACNNNNNGTC	2 cut(s)	130, 1044
EarI	CTCTTC	3 cut(s)	1514, 1600, 1707
EciI	GGCGGA	1 cut(s)	269
Eco130I	CCWWGG	2 cut(s)	393, 459
Eco32I	GATATC	1 cut(s)	1977
Eco57I	CTGAAG	3 cut(s)	650, 996, 1782
Eco72I	CACGTG	3 cut(s)	402, 952, 1173
Eco81I	CCTNAGG	1 cut(s)	2260
Eco88I	CYCGRG	2 cut(s)	760, 1328
EcoO109I	RGGNCCY	3 cut(s)	694, 2255, 2395
EcoRI	GAATTC	1 cut(s)	479
EcoRII	CCWGG	4 cut(s)	774, 1197, 1447, 1663
EcoRV	GATATC	1 cut(s)	1977
EcoT14I	CCWWGG	2 cut(s)	393, 459
EcoT22I	ATGCAT	2 cut(s)	1114, 1917
EgeI	GGCGCC	3 cut(s)	577, 664, 807
EheI	GGCGCC	3 cut(s)	577, 664, 807
ErhI	CCWWGG	2 cut(s)	393, 459
FalI	AAGNNNNNCTT	4 cut(s)	284, 316, 2243, 2275
FaqI	GGGAC	7 cut(s)	341, 727, 1181, 1319, 2241, 2362, 2613
FauI	CCCGC	8 cut(s)	51, 332, 511, 621, 1118, 1157, 2454, 2640
FbaI	TGATCA	2 cut(s)	1657, 2640
FblI	GTMKAC	3 cut(s)	1076, 1632, 2276
FspBI	CTAG	5 cut(s)	1424, 1548, 1812, 1892, 2243
GlaI	GCGC	9 cut(s)	105, 517, 577, 605, 664, 807, 1672, 2608, 2616
GsaI	CCCAGC	1 cut(s)	256
HaeII	RGCGCY	6 cut(s)	107, 579, 666, 809, 1674, 2610
HaeIII	GGCC	8 cut(s)	8, 398, 968, 1207, 1475, 1626, 1840, 2483
HgaI	GACGC	4 cut(s)	46, 248, 1048, 1894
HhaI	GCGC	9 cut(s)	106, 518, 578, 606, 665, 808, 1673, 2609, 2617
Hin1I	GRCGYC	6 cut(s)	57, 576, 663, 806, 1040, 1992
Hin6I	GCGC	9 cut(s)	104, 516, 576, 604, 663, 806, 1671, 2607, 2615
HinP1I	GCGC	9 cut(s)	104, 516, 576, 604, 663, 806, 1671, 2607, 2615
HincII	GTYRAC	1 cut(s)	2302
HindII	GTYRAC	1 cut(s)	2302
HindIII	AAGCTT	2 cut(s)	2087, 2327
HphI	GGTGA	8 cut(s)	313, 340, 448, 877, 1652, 1792, 2164, 2350
Hpy166II	GTNNAC	8 cut(s)	456, 1077, 1095, 1621, 1633, 2161, 2277, 2302
Hpy188III	TCNNGA	9 cut(s)	187, 421, 1049, 1540, 1582, 1920, 1988, 2167, 2591
Hpy8I	GTNNAC	8 cut(s)	456, 1077, 1095, 1621, 1633, 2161, 2277, 2302
HpyAV	CCTTC	7 cut(s)	79, 118, 310, 1546, 2028, 2386, 2528
HpyCH4III	ACNGT	4 cut(s)	418, 2211, 2438, 2549
HpyF3I	CTNAG	4 cut(s)	1323, 2181, 2260, 2576
Hsp92I	GRCGYC	6 cut(s)	57, 576, 663, 806, 1040, 1992
HspAI	GCGC	9 cut(s)	104, 516, 576, 604, 663, 806, 1671, 2607, 2615
KasI	GGCGCC	3 cut(s)	575, 662, 805
KflI	GGGWCCC	1 cut(s)	2255
Kpn2I	TCCGGA	1 cut(s)	186
KroI	GCCGGC	1 cut(s)	581
KroNI	GCCGGC	1 cut(s)	583
Ksp22I	TGATCA	2 cut(s)	1657, 2640
LmnI	GCTCC	6 cut(s)	71, 686, 796, 945, 961, 2581
Lsp1109I	GCAGC	6 cut(s)	660, 1264, 1900, 2127, 2445, 2668
MaeI	CTAG	5 cut(s)	1424, 1548, 1812, 1892, 2243
MaeIII	GTNAC	9 cut(s)	263, 502, 889, 1046, 1135, 1245, 1780, 2205, 2560
MbiI	CCGCTC	1 cut(s)	66
MboII	GAAGA	8 cut(s)	1141, 1531, 1587, 1724, 1866, 2456, 2477, 2683
MfeI	CAATTG	3 cut(s)	304, 2076, 2121
MhlI	GDGCHC	5 cut(s)	942, 1266, 2268, 2577, 2586
MlsI	TGGCCA	2 cut(s)	968, 1475
MluNI	TGGCCA	2 cut(s)	968, 1475
Mly113I	GGCGCC	3 cut(s)	576, 663, 806
MlyI	GAGTC	6 cut(s)	119, 1075, 1306, 1487, 1796, 2213
MmeI	TCCRAC	5 cut(s)	615, 839, 930, 1463, 1527
Mox20I	TGGCCA	2 cut(s)	968, 1475
Mph1103I	ATGCAT	2 cut(s)	1114, 1917
MroI	TCCGGA	1 cut(s)	186
MroNI	GCCGGC	1 cut(s)	581
MscI	TGGCCA	2 cut(s)	968, 1475
MseI	TTAA	3 cut(s)	182, 1008, 1101
MslI	CAYNNNNRTG	4 cut(s)	954, 1355, 1665, 2063
Msp20I	TGGCCA	2 cut(s)	968, 1475
MspA1I	CMGCKG	1 cut(s)	252
MspR9I	CCNGG	8 cut(s)	451, 702, 776, 843, 1199, 1449, 1665, 2353
MunI	CAATTG	3 cut(s)	304, 2076, 2121
Mva1269I	GAATGC	2 cut(s)	1680, 1915
MvaI	CCWGG	4 cut(s)	776, 1199, 1449, 1665
MvnI	CGCG	5 cut(s)	477, 518, 606, 1942, 2286
NaeI	GCCGGC	1 cut(s)	583
NarI	GGCGCC	3 cut(s)	576, 663, 806
NciI	CCSGG	4 cut(s)	451, 702, 843, 2353
NcoI	CCATGG	1 cut(s)	459
NgoMIV	GCCGGC	1 cut(s)	581
NheI	GCTAGC	1 cut(s)	1891
NmeAIII	GCCGAG	1 cut(s)	715
NmuCI	GTSAC	8 cut(s)	502, 889, 1046, 1135, 1245, 1780, 2205, 2560
NsiI	ATGCAT	2 cut(s)	1114, 1917
NspI	RCATGY	3 cut(s)	787, 1181, 2625
OliI	CACNNNNGTG	1 cut(s)	1665
PaeI	GCATGC	1 cut(s)	2625
PaeR7I	CTCGAG	1 cut(s)	760
PagI	TCATGA	1 cut(s)	1539
PcsI	WCGNNNNNNNCGW	4 cut(s)	120, 678, 687, 1034
PctI	GAATGC	2 cut(s)	1680, 1915
PdiI	GCCGGC	1 cut(s)	583
PfeI	GAWTC	9 cut(s)	342, 541, 642, 882, 902, 1499, 1767, 2007, 2556
PflMI	CCANNNNNTGG	1 cut(s)	2035
PinAI	ACCGGT	1 cut(s)	428
PleI	GAGTC	6 cut(s)	119, 1075, 1306, 1487, 1796, 2213
PluTI	GGCGCC	3 cut(s)	579, 666, 809
PmaCI	CACGTG	3 cut(s)	402, 952, 1173
PmlI	CACGTG	3 cut(s)	402, 952, 1173
PpsI	GAGTC	6 cut(s)	119, 1075, 1306, 1487, 1796, 2213
Ppu21I	YACGTR	3 cut(s)	402, 952, 1173
PpuMI	RGGWCCY	3 cut(s)	694, 2255, 2395
PshAI	GACNNNNGTC	1 cut(s)	509
PsiI	TTATAA	1 cut(s)	1034
Psp5II	RGGWCCY	3 cut(s)	694, 2255, 2395
Psp6I	CCWGG	4 cut(s)	774, 1197, 1447, 1663
PspCI	CACGTG	3 cut(s)	402, 952, 1173
PspFI	CCCAGC	1 cut(s)	252
PspGI	CCWGG	4 cut(s)	774, 1197, 1447, 1663
PspPPI	RGGWCCY	3 cut(s)	694, 2255, 2395
PspXI	VCTCGAGB	1 cut(s)	760
PvuII	CAGCTG	1 cut(s)	252
RseI	CAYNNNNRTG	4 cut(s)	954, 1355, 1665, 2063
SaqAI	TTAA	3 cut(s)	182, 1008, 1101
ScaI	AGTACT	1 cut(s)	1226
SchI	GAGTC	6 cut(s)	119, 1075, 1306, 1487, 1796, 2213
ScrFI	CCNGG	8 cut(s)	451, 702, 776, 843, 1199, 1449, 1665, 2353
SduI	GDGCHC	5 cut(s)	942, 1266, 2268, 2577, 2586
SfoI	GGCGCC	3 cut(s)	577, 664, 807
Sfr274I	CTCGAG	1 cut(s)	760
SlaI	CTCGAG	1 cut(s)	760
SmiMI	CAYNNNNRTG	4 cut(s)	954, 1355, 1665, 2063
SmlI	CTYRAG	2 cut(s)	733, 760
SmoI	CTYRAG	2 cut(s)	733, 760
SpeI	ACTAGT	1 cut(s)	1547
SphI	GCATGC	1 cut(s)	2625
SspDI	GGCGCC	3 cut(s)	575, 662, 805
SspMI	CTAG	5 cut(s)	1424, 1548, 1812, 1892, 2243
StyD4I	CCNGG	8 cut(s)	449, 700, 774, 841, 1197, 1447, 1663, 2351
StyI	CCWWGG	2 cut(s)	393, 459
TaaI	ACNGT	4 cut(s)	418, 2211, 2438, 2549
TaqII	GACCGA	1 cut(s)	2426
TatI	WGTACW	4 cut(s)	1224, 1563, 1831, 1858
TauI	GCSGC	5 cut(s)	444, 581, 609, 654, 1125
TfiI	GAWTC	9 cut(s)	342, 541, 642, 882, 902, 1499, 1767, 2007, 2556
Tru1I	TTAA	3 cut(s)	182, 1008, 1101
Tru9I	TTAA	3 cut(s)	182, 1008, 1101
TscAI	CASTG	5 cut(s)	292, 1254, 1705, 2229, 2554
TseFI	GTSAC	8 cut(s)	502, 889, 1046, 1135, 1245, 1780, 2205, 2560
TseI	GCWGC	6 cut(s)	648, 1277, 1888, 2115, 2458, 2656
Tsp45I	GTSAC	8 cut(s)	502, 889, 1046, 1135, 1245, 1780, 2205, 2560
TspDTI	ATGAA	9 cut(s)	249, 334, 838, 1376, 1528, 1556, 2142, 2685, 2690
TspGWI	ACGGA	4 cut(s)	191, 354, 1080, 2579
TspRI	CASTG	5 cut(s)	292, 1254, 1705, 2229, 2554
Van91I	CCANNNNNTGG	1 cut(s)	2035
XapI	RAATTY	4 cut(s)	479, 2645, 2662, 2692
XceI	RCATGY	3 cut(s)	787, 1181, 2625
XhoI	CTCGAG	1 cut(s)	760
XmiI	GTMKAC	3 cut(s)	1076, 1632, 2276
XspI	CTAG	5 cut(s)	1424, 1548, 1812, 1892, 2243
ZraI	GACGTC	1 cut(s)	1993
ZrmI	AGTACT	1 cut(s)	1226
Zsp2I	ATGCAT	2 cut(s)	1114, 1917

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.